Base de dados : MEDLINE
Pesquisa : D02.241.511.459.800 [Categoria DeCS]
Referências encontradas : 269 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 27 ir para página                         

  1 / 269 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28213034
[Au] Autor:Lourenço A; Kamnetz MB; Gadotti C; Diez-Gonzalez F
[Ad] Endereço:Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108, USA.
[Ti] Título:Antimicrobial treatments to control Listeria monocytogenes in queso fresco.
[So] Source:Food Microbiol;64:47-55, 2017 Jun.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Queso fresco, is a Hispanic non-fermented cheese highly susceptible to contamination with L. monocytogenes. This research was aimed to determine the effect of GRAS antimicrobial ingredients to control L. monocytogenes. Antimicrobials included caprylic acid (CA), Nisaplin (N, 2.5% nisin), a mixture of sodium lactate and sodium diacetate (SL/SD), Lactococcus lactis sbp. lactis DPC 3147, monolaurin, and lactic acid (LA). Batches of queso fresco curds were inoculated with 10 CFU/g and stored at 4 °C for three weeks. During storage the count of L. monocytogenes reached 7 to 8 Log CFU/g in control samples. Most individual antimicrobial treatments resulted in less than 1 Log CFU/g reductions in final counts, with the exception of N (0.5 g/kg) and CA (2.9 g/kg) that caused more than 3 and 5 Log CFU/g differences with controls, respectively. Mixtures of ingredients were more effective in inhibiting L. monocytogenes growth, and treatments with N and CA consistently delivered 6 Log CFU/g less counts than controls. Supplementation of 12 g/kg LA to treatments with SL/SD (3%/0.22%) caused differences of more than 4 Log CFU/g in final Listeria populations. Samples treated with the binary mixtures of N and CA (0.5 and 0.7 g/kg, respectively) were evaluated in a consumer panel (n = 67). Panelists slightly preferred control and commercial over treated samples, but all samples were in average rated between "slightly liking" and "moderately liking." These experiments indicated that combined use of antimicrobial ingredients may be an effective way to control the population of Listeria monocytogenes in queso fresco.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Queijo/microbiologia
Microbiologia de Alimentos
Conservantes de Alimentos/farmacologia
Listeria monocytogenes/efeitos dos fármacos
Listeria monocytogenes/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Acetatos/farmacologia
Caprilatos/farmacologia
Contagem de Colônia Microbiana
Comportamento do Consumidor
Segurança de Produtos ao Consumidor
Conservação de Alimentos/métodos
Nisina/farmacologia
Lactato de Sódio/farmacologia
Paladar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Anti-Bacterial Agents); 0 (Caprylates); 0 (Food Preservatives); 1414-45-5 (Nisin); 26WJH3CS0B (sodium diacetate); OBL58JN025 (octanoic acid); TU7HW0W0QT (Sodium Lactate)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170314
[Lr] Data última revisão:
170314
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170219
[St] Status:MEDLINE


  2 / 269 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28040163
[Au] Autor:Liu X; Basu U; Miller P; McMullen LM
[Ad] Endereço:Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
[Ti] Título:Differential gene expression and filamentation of Listeria monocytogenes 08-5923 exposed to sodium lactate and sodium diacetate.
[So] Source:Food Microbiol;63:153-158, 2017 May.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study reports the gene expression and filamentation in Listeria monocytogenes 08-5923 following exposure to food preservatives sodium lactate (NaL) and sodium diacetate (SD). L. monocytogenes 08-5923 was challenged with a mixture of NaL/SD, NaL or sodium acetate at 37 °C in tryptic soy broth. In the initial study, L. monocytogenes 08-5923 was exposed to NaL/SD for 24 h. The transcriptome was investigated by RNA sequencing. A stress response network was discovered in L. monocytogenes 08-5923, which is mediated by genes encoding two-component systems (hisJ, lisK, OmpR family gene, resE) and RNA polymerase factors (sigC, sigH). NaL/SD resulted in the down-regulation of genes in glycolysis (pykA, eno, fbaA, pgm) and up-regulation of genes in DNA repair (radC), cell division (ftsE) and cell structure synthesis (flagella synthesis: flgK, fliF, fliD). Filamentation was monitored by flow cytometry. NaL/SD mixture resulted in filamentation in L. monocytogenes 08-5923. Longer exposure was required to induce filamentation in L. monocytogenes for SD (24 h) than for NaL (8 h) when cells were exposed to individual salt. The quantitative real time PCR analysis revealed the down-regulation of ftsE in filamented cells of Listeria exposed to NaL or sodium acetate.
[Mh] Termos MeSH primário: Acetatos/farmacologia
Expressão Gênica
Redes Reguladoras de Genes
Listeria monocytogenes/efeitos dos fármacos
Listeria monocytogenes/genética
Lactato de Sódio/farmacologia
[Mh] Termos MeSH secundário: Carga Bacteriana
Proteínas de Bactérias/genética
Reparo do DNA/genética
Proteínas de Ligação a DNA/genética
Flagelos/genética
Microbiologia de Alimentos
Perfilação da Expressão Gênica
Glicólise/genética
Listeria monocytogenes/metabolismo
Listeria monocytogenes/fisiologia
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de RNA
Fator sigma/genética
Estresse Fisiológico/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Bacterial Proteins); 0 (DNA-Binding Proteins); 0 (SigH protein, bacteria); 0 (Sigma Factor); 0 (sigC protein, Bacteria); 26WJH3CS0B (sodium diacetate); TU7HW0W0QT (Sodium Lactate)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170307
[Lr] Data última revisão:
170307
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


  3 / 269 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27498351
[Au] Autor:Ben Salem C; Slim R; Fathallah N; Hmouda H
[Ad] Endereço:Department of Pharmacovigilance, Faculty of Medicine of Sousse and.
[Ti] Título:Drug-induced hyperuricaemia and gout.
[So] Source:Rheumatology (Oxford);56(5):679-688, 2017 May 01.
[Is] ISSN:1462-0332
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hyperuricaemia is a common clinical condition that can be defined as a serum uric acid level >6.8 mg/dl (404 µmol/l). Gout, a recognized complication of hyperuricaemia, is the most common inflammatory arthritis in adults. Drug-induced hyperuricaemia and gout present an emergent and increasingly prevalent problem in clinical practice. Diuretics are one of the most important causes of secondary hyperuricaemia. Drugs raise serum uric acid level by an increase of uric acid reabsorption and/or decrease in uric acid secretion. Several drugs may also increase uric acid production. In this review, drugs leading to hyperuricaemia are summarized with regard to their mechanism of action and clinical significance. Increased awareness of drugs that can induce hyperuricaemia and gout, and monitoring and prevention are key elements for reducing the morbidity related to drug-induced hyperuricaemia and gout.
[Mh] Termos MeSH primário: Hiperuricemia/induzido quimicamente
[Mh] Termos MeSH secundário: Androgênios/efeitos adversos
Antituberculosos/efeitos adversos
Aspirina/efeitos adversos
Carboidratos/efeitos adversos
Citotoxinas/efeitos adversos
Diuréticos/efeitos adversos
Gota/induzido quimicamente
Seres Humanos
Hiperuricemia/prevenção & controle
Imunossupressores/efeitos adversos
Niacina/efeitos adversos
Lactato de Sódio/efeitos adversos
Testosterona/efeitos adversos
Ácido Úrico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Androgens); 0 (Antitubercular Agents); 0 (Carbohydrates); 0 (Cytotoxins); 0 (Diuretics); 0 (Immunosuppressive Agents); 2679MF687A (Niacin); 268B43MJ25 (Uric Acid); 3XMK78S47O (Testosterone); R16CO5Y76E (Aspirin); TU7HW0W0QT (Sodium Lactate)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160808
[St] Status:MEDLINE
[do] DOI:10.1093/rheumatology/kew293


  4 / 269 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27999422
[Au] Autor:Yang SX; Chen YX; Xu J; Yang ZH
[Ad] Endereço:Department of Infectious Diseases, Linyi People's Hospital, Linyi, Shandong, China (mainland).
[Ti] Título:Plasma Intermedin Level Indicates Severity and Treatment Efficacy of Septic Shock in Sprague-Dawley (SD) Rats.
[So] Source:Med Sci Monit;22:5028-5034, 2016 Dec 21.
[Is] ISSN:1643-3750
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND The aim of this study was to investigate the value of plasma intermedin (IMD) in assessing severity and treatment efficacy of septic shock. MATERIAL AND METHODS Healthy male Sprague-Dawley (SD) rats were chosen and divided into a normal control group (n=15) and a shock model group (n=27) that received intravenous injection of lipopolysaccharide (LPS). Then, 3 specimens were taken from each group. The shock model group rats were divided into an LPS group and a treatment group with 12 rats each. The treatment group received intravenous injection of compound sodium lactate solution. Plasma IMD and IMD1-47 mRNA expressions were compared and analyzed. RESULTS Mean arterial pressure (MAP) was lower while white blood cell count and TNF-α were higher in the shock model group than in the normal control group (P<0.05). After 10 h and 20 h, the treatment group had lower plasma IMD and IMD1-47 mRNA expressions compared with the LPS group (P<0.05). Plasma IMD and IMD1-47 mRNA expressions in the LPS group after 20 h were significantly higher than after 10 h (P<0.05). IMD was positively correlated with interleukins (IL-3, IL-6, and IL-8), white blood cell count, and body temperature (all P<0.05), but were negatively correlated with systolic pressure (r=-0.8474, P=0.0040). CONCLUSIONS Plasma IMD level can effectively reflect the severity of septic shock and can be used as an important indicator of septic shock treatment effectiveness.
[Mh] Termos MeSH primário: Adrenomedulina/sangue
Neuropeptídeos/sangue
Choque Séptico/sangue
Choque Séptico/tratamento farmacológico
[Mh] Termos MeSH secundário: Adrenomedulina/genética
Animais
Biomarcadores Farmacológicos/sangue
Injeções Intravenosas
Interleucinas/sangue
Lipopolissacarídeos/administração & dosagem
Masculino
Neuropeptídeos/genética
RNA Mensageiro/biossíntese
RNA Mensageiro/genética
Ratos
Ratos Sprague-Dawley
Choque Séptico/induzido quimicamente
Lactato de Sódio/administração & dosagem
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Pharmacological); 0 (Interleukins); 0 (Lipopolysaccharides); 0 (Neuropeptides); 0 (RNA, Messenger); 0 (intermedin protein, rat); 148498-78-6 (Adrenomedullin); TU7HW0W0QT (Sodium Lactate)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE


  5 / 269 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27641098
[Au] Autor:Brand A; Singer K; Koehl GE; Kolitzus M; Schoenhammer G; Thiel A; Matos C; Bruss C; Klobuch S; Peter K; Kastenberger M; Bogdan C; Schleicher U; Mackensen A; Ullrich E; Fichtner-Feigl S; Kesselring R; Mack M; Ritter U; Schmid M; Blank C; Dettmer K; Oefner PJ; Hoffmann P; Walenta S; Geissler EK; Pouyssegur J; Villunger A; Steven A; Seliger B; Schreml S; Haferkamp S; Kohl E; Karrer S; Berneburg M; Herr W; Mueller-Klieser W; Renner K; Kreutz M
[Ad] Endereço:Department of Internal Medicine III, University Hospital Regensburg, 93053 Regensburg, Germany.
[Ti] Título:LDHA-Associated Lactic Acid Production Blunts Tumor Immunosurveillance by T and NK Cells.
[So] Source:Cell Metab;24(5):657-671, 2016 Nov 08.
[Is] ISSN:1932-7420
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Elevated lactate dehydrogenase A (LDHA) expression is associated with poor outcome in tumor patients. Here we show that LDHA-associated lactic acid accumulation in melanomas inhibits tumor surveillance by T and NK cells. In immunocompetent C57BL/6 mice, tumors with reduced lactic acid production (Ldha ) developed significantly slower than control tumors and showed increased infiltration with IFN-γ-producing T and NK cells. However, in Rag2 γc mice, lacking lymphocytes and NK cells, and in Ifng mice, Ldha and control cells formed tumors at similar rates. Pathophysiological concentrations of lactic acid prevented upregulation of nuclear factor of activated T cells (NFAT) in T and NK cells, resulting in diminished IFN-γ production. Database analyses revealed negative correlations between LDHA expression and T cell activation markers in human melanoma patients. Our results demonstrate that lactic acid is a potent inhibitor of function and survival of T and NK cells leading to tumor immune escape.
[Mh] Termos MeSH primário: Vigilância Imunológica
Células Matadoras Naturais/imunologia
L-Lactato Desidrogenase/metabolismo
Ácido Láctico/biossíntese
Melanoma/imunologia
Linfócitos T/imunologia
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Linfócitos T CD8-Positivos/imunologia
Contagem de Células
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Sobrevivência Celular/imunologia
Citocinas/biossíntese
Glicólise/efeitos dos fármacos
Seres Humanos
Vigilância Imunológica/efeitos dos fármacos
Interferon gama/farmacologia
Isoenzimas/metabolismo
Células Matadoras Naturais/efeitos dos fármacos
Ácido Láctico/farmacologia
Masculino
Melanoma/patologia
Camundongos Endogâmicos C57BL
Fatores de Transcrição NFATC/metabolismo
Fenótipo
Lactato de Sódio/farmacologia
Linfócitos T/efeitos dos fármacos
Regulação para Cima/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Isoenzymes); 0 (NFATC Transcription Factors); 33X04XA5AT (Lactic Acid); 82115-62-6 (Interferon-gamma); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 1.1.1.27.- (lactate dehydrogenase 5); TU7HW0W0QT (Sodium Lactate)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160920
[St] Status:MEDLINE


  6 / 269 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Tenuta, Lívia Maria Andaló
Cury, Jaime A
Texto completo
[PMID]:27355353
[Au] Autor:Souza JG; Tenuta LM; Del Bel Cury AA; Nóbrega DF; Budin RR; de Queiroz MX; Vogel GL; Cury JA
[Ad] Endereço:Piracicaba Dental School, UNICAMP, Piracicaba, Brazil.
[Ti] Título:Calcium Prerinse before Fluoride Rinse Reduces Enamel Demineralization: An in situ Caries Study.
[So] Source:Caries Res;50(4):372-7, 2016.
[Is] ISSN:1421-976X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:A calcium (Ca) prerinse before a fluoride (F) rinse has been shown to increase oral F levels. We tested the anticaries effect of this combination in a dose-response in situ caries model. In a double-blind, crossover experiment, 10 volunteers carried enamel slabs in palatal appliances for 14 days, during which they rinsed twice/day with one of four rinse combinations: (1) a placebo prerinse (150 mM sodium lactate) followed by a distilled water rinse (negative control); (2) a placebo prerinse followed by a 250 ppm F rinse; (3) a placebo prerinse followed by a 1,000 ppm F rinse, or (4) a Ca prerinse (150 mM Ca, as calcium lactate) followed by a 250 ppm F rinse. Sucrose solution was dripped onto the slabs 8×/day to simulate a high cariogenic challenge. The percent surface hardness loss (%SHL) was significantly lower in the Ca prerinse used with the 250 ppm F rinse group (%SHL = 38.0 ± 21.0) when compared with the F rinse alone (%SHL = 59.5 ± 24.1) and similar to the 1,000 ppm F rinse group (%SHL = 42.0 ± 18.3). Compared with the 250 ppm F rinse, the Ca prerinse increased biofilm fluid F only twice (nonsignificant). However, it greatly increased F in biofilm solids (∼22×). The Ca prerinse had little effect on loosely or firmly bound enamel F. The results showed an increased level of protection against demineralization by the use of a Ca prerinse, which seems to be caused by the enhancement of F concentration in the biofilm.
[Mh] Termos MeSH primário: Cálcio/farmacologia
Cariostáticos/farmacologia
Esmalte Dentário/efeitos dos fármacos
Fluoretos Tópicos/farmacologia
Antissépticos Bucais/administração & dosagem
Desmineralização do Dente/terapia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Biofilmes/efeitos dos fármacos
Cálcio/administração & dosagem
Cariostáticos/administração & dosagem
Bovinos
Estudos Cross-Over
Esmalte Dentário/patologia
Relação Dose-Resposta a Droga
Método Duplo-Cego
Fluoretos Tópicos/administração & dosagem
Seres Humanos
Antissépticos Bucais/farmacologia
Saliva/efeitos dos fármacos
Lactato de Sódio/administração & dosagem
Lactato de Sódio/farmacologia
Sacarose/efeitos adversos
Fatores de Tempo
Desmineralização do Dente/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cariostatic Agents); 0 (Fluorides, Topical); 0 (Mouthwashes); 57-50-1 (Sucrose); SY7Q814VUP (Calcium); TU7HW0W0QT (Sodium Lactate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:160630
[St] Status:MEDLINE
[do] DOI:10.1159/000446407


  7 / 269 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27258789
[Au] Autor:Broady JW; Han D; Yuan J; Liao C; Bratcher CL; Lilies MR; Schwartz EH; Wang L
[Ad] Endereço:Dept. of Animal Sciences, Auburn Univ, Auburn, AL, 36849.
[Ti] Título:Survival and Metabolic Activity of Listeria monocytogenes on Ready-to-Eat Roast Beef Stored at 4 °C.
[So] Source:J Food Sci;81(7):M1766-72, 2016 Jul.
[Is] ISSN:1750-3841
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Three brands of commercial roast beef were purchased and artificially inoculated with a 5-strain Listeria monocytogenes cocktail at 2 inoculation levels (approximately 3 and 6 Log CFU/g). Although all 3 brands contained sodium diacetate and sodium lactate, inoculated Listeria cocktail survived for 16 d in all 3 brands; significant increases in L. monocytogenes numbers were seen on inoculated Brand B roast beef on days 12 and 16. Numbers of L. monocytogenes increased to 4.14 Log CFU/g for the 3 Log CFU/g inoculation level and increased to 7.99 Log CFU/g for the 6 Log CFU/g inoculation level by day 16, with the pH values being 5.4 and 5.8 respectively. To measure the cell viability in potential biofilms formed, an Alamar blue assay was conducted. Brand B meat homogenate had the highest metabolic activities (P < 0.05). By comparing its metabolic activities to Brands A and C and the inoculated autoclaved meat homogenates, results indicated that the microflora present in Brand B may be the reason for high metabolic activities. Based on the denaturing gradient gel electrophoresis and the Shannon-Wiener diversity index analysis, the "Brand" factor significantly impacted the diversity index (P = 0.012) and Brand B had the highest microflora diversity (Shannon index 1.636 ± 0.011). Based on this study, results showed that antimicrobials cannot completely inhibit the growth of L. monocytogenes in ready-to-eat roast beef. Native microflora (both diversity and abundance), together with product formula, pH, antimicrobial concentrations, and storage conditions may all impact the survival and growth of L. monocytogenes.
[Mh] Termos MeSH primário: Microbiologia de Alimentos
Conservação de Alimentos/métodos
Armazenamento de Alimentos/métodos
Listeria monocytogenes/crescimento & desenvolvimento
Listeria monocytogenes/metabolismo
Carne Vermelha/microbiologia
[Mh] Termos MeSH secundário: Acetatos
Animais
Anti-Infecciosos
Biodiversidade
Biofilmes
Bovinos
Contagem de Colônia Microbiana
Conservantes de Alimentos
Seres Humanos
Concentração de Íons de Hidrogênio
Produtos da Carne/microbiologia
Lactato de Sódio
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Anti-Infective Agents); 0 (Food Preservatives); 26WJH3CS0B (sodium diacetate); TU7HW0W0QT (Sodium Lactate)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160604
[St] Status:MEDLINE
[do] DOI:10.1111/1750-3841.13347


  8 / 269 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27236848
[Au] Autor:Dolinová I; Czinnerová M; Dvorák L; Stejskal V; Sevcu A; Cerník M
[Ad] Endereço:Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Studentská 2, 461 17, Liberec, Czech Republic. Electronic address: iva.dolinova@tul.cz.
[Ti] Título:Dynamics of organohalide-respiring bacteria and their genes following in-situ chemical oxidation of chlorinated ethenes and biostimulation.
[So] Source:Chemosphere;157:276-85, 2016 Aug.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Application of Fenton's reagent and enhanced reductive dechlorination are currently the most common remediation strategies resulting in removal of chlorinated ethenes. In this study, the influence of such techniques on organohalide-respiring bacteria was assessed at a site contaminated by chlorinated ethenes using a wide spectrum of molecular genetic markers, including 16S rRNA gene of the organohalide-respiring bacteria Dehaloccocoides spp., Desulfitobacterium and Dehalobacter; reductive dehalogenase genes (vcrA, bvcA) responsible for dechlorination of vinyl chloride and sulphate-reducing and denitrifying bacteria. In-situ application of hydrogen peroxide to induce a Fenton-like reaction caused an instantaneous decline in all markers below detection limit. Two weeks after application, the bvcA gene and Desulfitobacterium relative abundance increased to levels significantly higher than those prior to application. No significant decrease in the concentration of a range of chlorinated ethenes was observed due to the low hydrogen peroxide dose used. A clear increase in marker levels was also observed following in-situ application of sodium lactate, which resulted in a seven-fold increase in Desulfitobacterium and a three-fold increase in Dehaloccocoides spp. after 70 days. An increase in the vcrA gene corresponded with increase in Dehaloccocoides spp. Analysis of selected markers clearly revealed a positive response of organohalide-respiring bacteria to biostimulation and unexpectedly fast recovery after the Fenton-like reaction.
[Mh] Termos MeSH primário: Bactérias/metabolismo
Catálise
Cloro/metabolismo
Hidrocarbonetos Clorados/metabolismo
Lactato de Sódio/metabolismo
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Marcadores Genéticos/genética
Oxirredução
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Lactato de Sódio/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers); 0 (Hydrocarbons, Chlorinated); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 0 (Water Pollutants, Chemical); 4R7X1O2820 (Chlorine); TU7HW0W0QT (Sodium Lactate)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160530
[St] Status:MEDLINE


  9 / 269 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26841889
[Au] Autor:Fu T; Zhang C; Jing Y; Jiang C; Li Z; Wang S; Ma K; Zhang D; Hou S; Dai J; Kou G; Wang H
[Ad] Endereço:International Joint Cancer Institute, The Second Military Medical University, Shanghai, 200433, China.
[Ti] Título:Regulation of cell growth and apoptosis through lactate dehydrogenase C over-expression in Chinese hamster ovary cells.
[So] Source:Appl Microbiol Biotechnol;100(11):5007-16, 2016 Jun.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Lactate has long been credited as a by-product, which jeopardizes cell growth and productivity when accumulated over a certain concentration during the manufacturing process of therapeutic recombinant proteins by Chinese hamster ovary (CHO) cells. A number of efforts to decrease the lactate concentration have been developed; however, the accumulation of lactate is still a critical issue by the late stage of fed-batch culture. Therefore, a lactate-tolerant cell line was developed through over-expression of lactate dehydrogenase C (LDH-C). In fed-batch culture, sodium lactate or sodium pyruvate was supplemented into the culture medium to simulate the environment of lactate accumulation, and LDH-C over-expression increased the highest viable cell density by over 30 and 50 %, respectively, on day 5, meanwhile the viability was also improved significantly since day 5 compared with that of the control. The percentages of cells suffering early and late apoptosis decreased by 3.2 to 12.5 and 2.0 to 4.3 %, respectively, from day 6 onwards in the fed-batch culture when 40 mM sodium pyruvate was added compared to the control. The results were confirmed by mitochondrial membrane potential assay. In addition, the expression of cleaved caspases 3 and 7 decreased in cells over-expressing LDH-C, suggesting the mitochondrial pathway was involved in the LDH-C regulated anti-apoptosis. In conclusion, a novel cell line with higher lactate tolerance, lowered lactate production, and alleviated apoptosis response was developed by over-expression of LDH-C, which may potentially represent an efficient and labor-saving approach in generating recombinant proteins.
[Mh] Termos MeSH primário: Apoptose
Proliferação Celular
Regulação da Expressão Gênica
L-Lactato Desidrogenase/genética
[Mh] Termos MeSH secundário: Animais
Técnicas de Cultura Celular por Lotes
Células CHO
Caspase 3/genética
Caspase 3/metabolismo
Caspase 7/genética
Caspase 7/metabolismo
Sobrevivência Celular
Clonagem Molecular
Cricetinae
Cricetulus
Meios de Cultura/química
Isoenzimas/genética
Isoenzimas/metabolismo
L-Lactato Desidrogenase/metabolismo
Potencial da Membrana Mitocondrial
Ácido Pirúvico/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Lactato de Sódio/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Isoenzymes); 0 (Recombinant Proteins); 8558G7RUTR (Pyruvic Acid); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 1.1.1.27.- (lactate dehydrogenase C4); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 7); TU7HW0W0QT (Sodium Lactate)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170207
[Lr] Data última revisão:
170207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160205
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-016-7348-4


  10 / 269 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26655973
[Au] Autor:Aramendi I; Manzanares W; Biestro A
[Ad] Endereço:Cátedra de Medicina Intensiva, Centro de Tratamiento Intensivo, Hospital de Clínicas Dr. Manuel Quintela, Facultad de Medicina, Universidad de la República (UdeLaR), Montevideo, Uruguay. Electronic address: nachoaramendi@gmail.com.
[Ti] Título:[Half-molar sodium-lactate: The osmotic agent we are looking for?].
[Ti] Título:Lactato de sodio 0,5 molar: ¿el agente osmótico que buscamos?.
[So] Source:Med Intensiva;40(2):113-7, 2016 Mar.
[Is] ISSN:1578-6749
[Cp] País de publicação:Spain
[La] Idioma:spa
[Ab] Resumo:Intracranial hypertension (ICH) is the most important modifiable factor with predictive negative value in brain injury patients. Osmotherapy is the most important first level specific measure in the treatment of ICH. Mannitol 20%, and 3, 7.5, 10, and 23% hypertonic sodium chloride are the most commonly used osmotic agents in the neurocritical care setting. Currently, controversy about the best osmotic agent remains elusive. Therefore, over the past few years, half-molar sodium lactate has been introduced as a new osmotic agent to be administered in the critically ill. Lactate is able to prevent hyperchloremia, as well as its adverse effects such as hyperchloremic acidosis, systemic inflammation, and acute kidney injury. Furthermore, lactate may also be used by glia as energy substrate in brain injury patients. Half-molar sodium lactate would also have a more potent and long-lasting effect decreasing intracellular osmolarity and by inhibiting neuronal volume control mechanisms. Pioneering researches in patients with traumatic brain injury have shown a more significant effect than mannitol on the control of ICH. In addition, in this group of patients this solution appears to be beneficial in preventing episodes of ICH. However, future research is necessary to corroborate or not these promising results.
[Mh] Termos MeSH primário: Lesões Encefálicas/terapia
Hipertensão Intracraniana/terapia
Lactato de Sódio
[Mh] Termos MeSH secundário: Seres Humanos
Manitol
Sódio
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
3OWL53L36A (Mannitol); 9NEZ333N27 (Sodium); TU7HW0W0QT (Sodium Lactate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151215
[St] Status:MEDLINE



página 1 de 27 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde