Base de dados : MEDLINE
Pesquisa : D02.251.500 [Categoria DeCS]
Referências encontradas : 73 [refinar]
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[PMID]:28541438
[Au] Autor:Kristoffersen EL; Givskov A; Jørgensen LA; Jensen PW; W Byl JA; Osheroff N; Andersen AH; Stougaard M; Ho YP; Knudsen BR
[Ad] Endereço:Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
[Ti] Título:Interlinked DNA nano-circles for measuring topoisomerase II activity at the level of single decatenation events.
[So] Source:Nucleic Acids Res;45(13):7855-7869, 2017 Jul 27.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:DNA nano-structures present appealing new means for monitoring different molecules. Here, we demonstrate the assembly and utilization of a surface-attached double-stranded DNA catenane composed of two intact interlinked DNA nano-circles for specific and sensitive measurements of the life essential topoisomerase II (Topo II) enzyme activity. Topo II activity was detected via the numeric release of DNA nano-circles, which were visualized at the single-molecule level in a fluorescence microscope upon isothermal amplification and fluorescence labeling. The transition of each enzymatic reaction to a micrometer sized labeled product enabled quantitative detection of Topo II activity at the single decatenation event level rendering activity measurements in extracts from as few as five cells possible. Topo II activity is a suggested predictive marker in cancer therapy and, consequently, the described highly sensitive monitoring of Topo II activity may add considerably to the toolbox of individualized medicine where decisions are based on very sparse samples.
[Mh] Termos MeSH primário: DNA Topoisomerases Tipo II/metabolismo
DNA Catenado/química
DNA Catenado/metabolismo
[Mh] Termos MeSH secundário: Antígenos de Neoplasias/análise
Antígenos de Neoplasias/metabolismo
Sequência de Bases
DNA Topoisomerases Tipo II/análise
DNA Catenado/genética
Proteínas de Ligação a DNA/análise
Proteínas de Ligação a DNA/metabolismo
Células HeLa
Seres Humanos
Proteínas Recombinantes/análise
Proteínas Recombinantes/metabolismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Neoplasm); 0 (DNA, Catenated); 0 (DNA-Binding Proteins); 0 (Recombinant Proteins); EC 5.99.1.3 (DNA Topoisomerases, Type II)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx480


  2 / 73 MEDLINE  
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[PMID]:27697840
[Au] Autor:Bahng S; Hayama R; Marians KJ
[Ad] Endereço:From the Molecular Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10065.
[Ti] Título:MukB-mediated Catenation of DNA Is ATP and MukEF Independent.
[So] Source:J Biol Chem;291(46):23999-24008, 2016 Nov 11.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Properly condensed chromosomes are necessary for accurate segregation of the sisters after DNA replication. The Escherichia coli condesin is MukB, a structural maintenance of chromosomes (SMC)-like protein, which forms a complex with MukE and the kleisin MukF. MukB is known to be able to mediate knotting of a DNA ring, an intramolecular reaction. In our investigations of how MukB condenses DNA we discovered that it can also mediate catenation of two DNA rings, an intermolecular reaction. This activity of MukB requires DNA binding by the head domains of the protein but does not require either ATP or its partner proteins MukE or MukF. The ability of MukB to mediate DNA catenation underscores its potential for bringing distal regions of a chromosome together.
[Mh] Termos MeSH primário: Proteínas Cromossômicas não Histona/metabolismo
DNA Bacteriano/metabolismo
DNA Catenado/metabolismo
Proteínas de Escherichia coli/metabolismo
Escherichia coli/metabolismo
Proteínas Repressoras/metabolismo
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/química
Trifosfato de Adenosina/genética
Trifosfato de Adenosina/metabolismo
Proteínas Cromossômicas não Histona/química
Proteínas Cromossômicas não Histona/genética
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Catenado/química
DNA Catenado/genética
Escherichia coli/química
Escherichia coli/genética
Proteínas de Escherichia coli/química
Proteínas de Escherichia coli/genética
Proteínas Repressoras/química
Proteínas Repressoras/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromosomal Proteins, Non-Histone); 0 (DNA, Bacterial); 0 (DNA, Catenated); 0 (Escherichia coli Proteins); 0 (MukB protein, E coli); 0 (Repressor Proteins); 0 (mukE protein, E coli); 0 (mukF protein, E coli); 8L70Q75FXE (Adenosine Triphosphate)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161005
[St] Status:MEDLINE


  3 / 73 MEDLINE  
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[PMID]:27214092
[Au] Autor:Li Q; Wu G; Wu W; Liang X
[Ad] Endereço:College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
[Ti] Título:Efficient Synthesis of Topologically Linked Three-Ring DNA Catenanes.
[So] Source:Chembiochem;17(12):1127-31, 2016 06 16.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Topologically controlled DNA catenanes are promising elements for the construction of molecular machines but present a significant effort in DNA nanotechnology. We report an efficient approach for preparing linear three-ring catenanes (L3C) composed of single-stranded DNA. The linking number was strictly controlled by using short complementary regions (6 nt) between each two DNA rings. High efficiency of forming three-ring catenanes (yield as high as 63 %) was obtained by using an 80 nt oligonucleotide as the scaffold to draw close the three pre-rings for hybridization between short complementary DNA. After assembly, three pre-rings were closed by DNA ligation using three 12 nt oligonucleotides as splints to form interlocked three-ring catenanes. L3C nanostructures were imaged in air by AFM: the catenane exhibited a smooth circular shape and was arranged in a line with well-defined structure, as expected.
[Mh] Termos MeSH primário: DNA Catenado/química
Nanoestruturas/química
[Mh] Termos MeSH secundário: DNA Catenado/síntese química
DNA de Cadeia Simples/química
Microscopia de Força Atômica
Hibridização de Ácido Nucleico
Oligonucleotídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Catenated); 0 (DNA, Single-Stranded); 0 (Oligonucleotides)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160524
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201600071


  4 / 73 MEDLINE  
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[PMID]:26994736
[Au] Autor:Wu ZS; Shen Z; Tram K; Salena BJ; Li Y
[Ad] Endereço:Departments of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.
[Ti] Título:Topological DNA Assemblies Containing Identical or Fraternal Twins.
[So] Source:Chembiochem;17(12):1142-5, 2016 06 16.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:DNA catenanes are assemblies made up of two or more DNA rings linked together through mechanical bonds, and they are desirable for engineering unique nanoscale devices. However, current methods of synthesizing DNA catenanes rely on the formation of strong linking duplexes between component units to enable interlocking and thus do not permit the synthesis of complex single-stranded DNA structures with freely functioning units. We have recently reported DNA sequences that can thread through a DNA circle without the formation of a linking duplex. Here we show that these unique DNA molecules can be further used to make intricate symmetric or asymmetric DNA [3]catenanes, single-stranded DNA assemblies made up of a central mother ring interlocked to two identical or fraternal twin daughter rings, which have never been reported before. These addressable freely functioning interlocked DNA rings should facilitate the design of elaborate nanoscale machines based on DNA.
[Mh] Termos MeSH primário: DNA Catenado/química
[Mh] Termos MeSH secundário: Enzimas de Restrição do DNA
DNA Catenado/síntese química
DNA Catenado/metabolismo
Eletroforese em Gel de Poliacrilamida
Nanoestruturas/química
Técnicas de Amplificação de Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Catenated); EC 3.1.21.- (DNA Restriction Enzymes)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160321
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201600036


  5 / 73 MEDLINE  
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[PMID]:26527691
[Au] Autor:Kawano S; Kato Y; Okada N; Sano K; Tsutsui K; Tsutsui KM; Ikeda S
[Ad] Endereço:Department of Biochemistry, Faculty of Science, Okayama University of Science, 1-1 Ridai-cho, Kita-ku, Okayama 700-0005, Japan and kawanos@dbc.ous.ac.jp.
[Ti] Título:DNA-binding activity of rat DNA topoisomerase II α C-terminal domain contributes to efficient DNA catenation in vitro.
[So] Source:J Biochem;159(3):363-9, 2016 Mar.
[Is] ISSN:1756-2651
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:DNA topoisomerase IIα (topo IIα) is an essential enzyme for resolution of DNA topologies arising in DNA metabolic reactions. In proliferating cells, topo II activities of DNA catenation or decatenation are required for condensation of chromosomes and segregation of chromatids. Recent studies suggest that the C-terminal domain (CTD) of human topo IIα is required for localization to mitotic chromosomes. Here, we show that the CTD of topo IIα is also associated with efficient DNA catenation in vitro, based on comparison of wild-type (WT) rat topo IIα and its deletion mutants. Unlike WT, the CTD truncated mutant (ΔCTD) lacked linear DNA binding activity, but could bind to negatively supercoiled DNA similarly to WT. The CTD alone showed linear DNA-binding activity. ΔCTD mediated formation of a DNA catenane in the presence of polyethylene glycol, which enhances macromolecular association. These results indicate that DNA-binding activity in the CTD of topo IIα concentrates the enzyme in the vicinity of condensed DNA and allows topo IIα to efficiently form a DNA catenane.
[Mh] Termos MeSH primário: Antígenos de Neoplasias/química
DNA Topoisomerases Tipo II/química
DNA Catenado/química
Proteínas de Ligação a DNA/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Antígenos de Neoplasias/genética
DNA Topoisomerases Tipo II/genética
Proteínas de Ligação a DNA/genética
Células HEK293
Seres Humanos
Polietilenoglicóis/metabolismo
Ligação Proteica
Estrutura Terciária de Proteína
Ratos
Deleção de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Neoplasm); 0 (DNA, Catenated); 0 (DNA-Binding Proteins); 30IQX730WE (Polyethylene Glycols); EC 5.99.1.3 (DNA Topoisomerases, Type II)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151104
[St] Status:MEDLINE
[do] DOI:10.1093/jb/mvv110


  6 / 73 MEDLINE  
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[PMID]:26364680
[Au] Autor:Ohayon YP; Sha R; Flint O; Chandrasekaran AR; Abdallah HO; Wang T; Wang X; Zhang X; Seeman NC
[Ad] Endereço:Department of Chemistry, New York University , New York, New York 10003, United States.
[Ti] Título:Topological Linkage of DNA Tiles Bonded by Paranemic Cohesion.
[So] Source:ACS Nano;9(10):10296-303, 2015 Oct 27.
[Is] ISSN:1936-086X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Catenation is the process by which cyclic strands are combined like the links of a chain, whereas knotting changes the linking properties of a single strand. In the cell, topoisomerases catalyzing strand passage operations enable the knotting and catenation of DNA so that single- or double-stranded segments can be passed through each other. Here, we use a system of closed DNA structures involving a paranemic motif, called PX-DNA, to bind double strands of DNA together. These PX-cohesive closed molecules contain complementary loops whose linking by Escherichia coli topoisomerase 1 (Topo 1) leads to various types of catenated and knotted structures. We were able to obtain specific DNA topological constructs by varying the lengths of the complementary tracts between the complementary loops. The formation of the structures was analyzed by denaturing gel electrophoresis, and the various topologies of the constructs were characterized using the program Knotilus.
[Mh] Termos MeSH primário: DNA Catenado/química
DNA/química
[Mh] Termos MeSH secundário: DNA/metabolismo
DNA Topoisomerases Tipo I/metabolismo
DNA Catenado/metabolismo
Escherichia coli/enzimologia
Ligações de Hidrogênio
Modelos Moleculares
Conformação de Ácido Nucleico
Desnaturação de Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Catenated); 9007-49-2 (DNA); EC 5.99.1.2 (DNA Topoisomerases, Type I)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151027
[Lr] Data última revisão:
151027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150915
[St] Status:MEDLINE
[do] DOI:10.1021/acsnano.5b04333


  7 / 73 MEDLINE  
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[PMID]:26343906
[Au] Autor:Ohayon YP; Sha R; Flint O; Liu W; Chakraborty B; Subramanian HK; Zheng J; Chandrasekaran AR; Abdallah HO; Wang X; Zhang X; Seeman NC
[Ad] Endereço:Department of Chemistry, New York University , New York, New York 10003, United States.
[Ti] Título:Covalent Linkage of One-Dimensional DNA Arrays Bonded by Paranemic Cohesion.
[So] Source:ACS Nano;9(10):10304-12, 2015 Oct 27.
[Is] ISSN:1936-086X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The construction of DNA nanostructures from branched DNA motifs, or tiles, typically relies on the use of sticky-ended cohesion, owing to the specificity and programmability of DNA sequences. The stability of such constructs when unligated is restricted to a specific range of temperatures, owing to the disruption of base pairing at elevated temperatures. Paranemic (PX) cohesion was developed as an alternative to sticky ends for the cohesion of large topologically closed species that could be purified reliably on denaturing gels. However, PX cohesion is also of limited stability. In this work, we added sticky-ended interactions to PX-cohesive complexes to create interlocked complexes by functionalizing the sticky ends with psoralen, which can form cross-links between the two strands of a double helix. We were able to reinforce the stability of the constructs by creating covalent linkages between the 3'-ends and 5'-ends of the sticky ends; the sticky ends were added to double crossover domains via 3'-3' and 5'-5' linkages. Catenated arrays were obtained either by enzymatic ligation or by UV cross-linking. We have constructed finite-length one-dimensional arrays linked by interlocking loops and have positioned streptavidin-gold particles on these constructs.
[Mh] Termos MeSH primário: DNA Catenado/química
Nanoestruturas/química
Análise de Sequência com Séries de Oligonucleotídeos
[Mh] Termos MeSH secundário: Pareamento de Bases
DNA Topoisomerases Tipo I/metabolismo
DNA Catenado/metabolismo
Escherichia coli/enzimologia
Ouro/química
Modelos Moleculares
Nanoestruturas/ultraestrutura
Nanotecnologia/métodos
Conformação de Ácido Nucleico
Motivos de Nucleotídeos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Catenated); 7440-57-5 (Gold); EC 5.99.1.2 (DNA Topoisomerases, Type I)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151027
[Lr] Data última revisão:
151027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150908
[St] Status:MEDLINE
[do] DOI:10.1021/acsnano.5b04335


  8 / 73 MEDLINE  
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[PMID]:26299966
[Au] Autor:Kanno T; Berta DG; Sjögren C
[Ad] Endereço:Department of Cell and Molecular Biology, Karolinska Institutet, von Eulers väg 3, 171 77 Stockholm, Sweden.
[Ti] Título:The Smc5/6 Complex Is an ATP-Dependent Intermolecular DNA Linker.
[So] Source:Cell Rep;12(9):1471-82, 2015 Sep 01.
[Is] ISSN:2211-1247
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The structural maintenance of chromosome (SMC) protein complexes cohesin and condensin and the Smc5/6 complex (Smc5/6) are crucial for chromosome dynamics and stability. All contain essential ATPase domains, and cohesin and condensin interact with chromosomes through topological entrapment of DNA. However, how Smc5/6 binds DNA and chromosomes has remained largely unknown. Here, we show that purified Smc5/6 binds DNA through a mechanism that requires ATP hydrolysis by the complex and circular DNA to be established. This also promotes topoisomerase 2-dependent catenation of plasmids, suggesting that Smc5/6 interconnects two DNA molecules using ATP-regulated topological entrapment of DNA, similar to cohesin. We also show that a complex containing an Smc6 mutant that is defective in ATP binding fails to interact with DNA and chromosomes and leads to cell death with concomitant accumulation of DNA damage when overexpressed. Taken together, these results indicate that Smc5/6 executes its cellular functions through ATP-regulated intermolecular DNA linking.
[Mh] Termos MeSH primário: Proteínas de Ciclo Celular/metabolismo
DNA Catenado/metabolismo
DNA Fúngico/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/metabolismo
Antígenos de Neoplasias/metabolismo
Proteínas de Ciclo Celular/genética
DNA Topoisomerases Tipo II/metabolismo
Proteínas de Ligação a DNA/metabolismo
Ligação Proteica
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
Proteínas de Saccharomyces cerevisiae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Neoplasm); 0 (Cell Cycle Proteins); 0 (DNA, Catenated); 0 (DNA, Fungal); 0 (DNA-Binding Proteins); 0 (SMC5 protein, S cerevisiae); 0 (SMC6 protein, S cerevisiae); 0 (Saccharomyces cerevisiae Proteins); 8L70Q75FXE (Adenosine Triphosphate); EC 5.99.1.3 (DNA Topoisomerases, Type II)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150825
[St] Status:MEDLINE


  9 / 73 MEDLINE  
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[PMID]:26150424
[Au] Autor:Racko D; Benedetti F; Dorier J; Burnier Y; Stasiak A
[Ad] Endereço:Center for Integrative Genomics, University of Lausanne, 1015-Lausanne, Switzerland SIB Swiss Institute of Bioinformatics, 1015-Lausanne, Switzerland Polymer Institute of the Slovak Academy of Sciences, 842 36 Bratislava, Slovakia.
[Ti] Título:Generation of supercoils in nicked and gapped DNA drives DNA unknotting and postreplicative decatenation.
[So] Source:Nucleic Acids Res;43(15):7229-36, 2015 Sep 03.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Due to the helical structure of DNA the process of DNA replication is topologically complex. Freshly replicated DNA molecules are catenated with each other and are frequently knotted. For proper functioning of DNA it is necessary to remove all of these entanglements. This is done by DNA topoisomerases that pass DNA segments through each other. However, it has been a riddle how DNA topoisomerases select the sites of their action. In highly crowded DNA in living cells random passages between contacting segments would only increase the extent of entanglement. Using molecular dynamics simulations we observed that in actively supercoiled DNA molecules the entanglements resulting from DNA knotting or catenation spontaneously approach sites of nicks and gaps in the DNA. Type I topoisomerases, that preferentially act at sites of nick and gaps, are thus naturally provided with DNA-DNA juxtapositions where a passage results in an error-free DNA unknotting or DNA decatenation.
[Mh] Termos MeSH primário: DNA Catenado/química
DNA Super-Helicoidal/química
[Mh] Termos MeSH secundário: DNA/química
Replicação do DNA
DNA Topoisomerases Tipo I/metabolismo
DNA Catenado/metabolismo
DNA Circular/química
DNA Super-Helicoidal/metabolismo
Simulação de Dinâmica Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Catenated); 0 (DNA, Circular); 0 (DNA, Superhelical); 9007-49-2 (DNA); EC 5.99.1.2 (DNA Topoisomerases, Type I); EC 5.99.1.2 (DNA topoisomerase III)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150831
[Lr] Data última revisão:
150831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150708
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkv683


  10 / 73 MEDLINE  
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[PMID]:25980669
[Au] Autor:Cassinelli V; Oberleitner B; Sobotta J; Nickels P; Grossi G; Kempter S; Frischmuth T; Liedl T; Manetto A
[Ad] Endereço:baseclick GmbH, Bahnhofstrasse 9-15, 82327 Tutzing (Germany).
[Ti] Título:One-Step Formation of "Chain-Armor"-Stabilized DNA Nanostructures.
[So] Source:Angew Chem Int Ed Engl;54(27):7795-8, 2015 Jun 26.
[Is] ISSN:1521-3773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:DNA-based self-assembled nanostructures are widely used to position organic and inorganic objects with nanoscale precision. A particular promising application of DNA structures is their usage as programmable carrier systems for targeted drug delivery. To provide DNA-based templates that are robust against degradation at elevated temperatures, low ion concentrations, adverse pH conditions, and DNases, we built 6-helix DNA tile tubes consisting of 24 oligonucleotides carrying alkyne groups on their 3'-ends and azides on their 5'-ends. By a mild click reaction, the two ends of selected oligonucleotides were covalently connected to form rings and interlocked DNA single strands, so-called DNA catenanes. Strikingly, the structures stayed topologically intact in pure water and even after precipitation from EtOH. The structures even withstood a temperature of 95 °C when all of the 24 strands were chemically interlocked.
[Mh] Termos MeSH primário: Alquinos/química
Azidas/química
DNA/química
Nanotubos/química
[Mh] Termos MeSH secundário: Química Click
DNA Catenado/química
Temperatura Alta
Nanotecnologia
Nanotubos/ultraestrutura
Oligonucleotídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkynes); 0 (Azides); 0 (DNA, Catenated); 0 (Oligonucleotides); 9007-49-2 (DNA)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150625
[Lr] Data última revisão:
150625
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150519
[St] Status:MEDLINE
[do] DOI:10.1002/anie.201500561



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