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  1 / 8930 MEDLINE  
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[PMID]:29390403
[Au] Autor:Xu Q; Liu Q; Ge H; Ge X; Wu J; Qu J; Xu K
[Ad] Endereço:The First School of Clinical Medicine, Nanjing Medical University.
[Ti] Título:Tumor recurrence versus treatment effects in glioma: A comparative study of three dimensional pseudo-continuous arterial spin labeling and dynamic susceptibility contrast imaging.
[So] Source:Medicine (Baltimore);96(50):e9332, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Gliomas constitute over 90% of primary brain tumors. Accurate identification of glioma recurrence and treatment effects is important, as it can help determine whether to continue with standard adjuvant chemotherapy or to switch to a second-line therapy for recurrence. Our purpose is to compare three dimensional pseudo-continuous arterial spin labeling (3D-pcASL) technique and dynamic susceptibility contrast perfusion magnetic resonance imaging (DSC-MRI) for differentiation tumor recurrence from treatment-related effects in gliomas. METHODS: Twenty-nine patients with gliomas previously who showed enlarged, contrast-enhancing lesions within the radiation field after surgery and concurrent chemoradiotherapy (CCRT) were assessed with 3D-pcASL and DSC-MRI. These patients were classified into 2 groups, tumor recurrence group (n = 17) and treatment effects group (n = 12), based on pathologic analysis or clinical-radiologic follow-up. The perfusion imaging quality was assessed using a 3-point scale (1 = poor imaging, 2 = moderate imaging, and 3 = good imaging). Comparison for perfusion imaging-quality score between the 2 techniques was performed with Wilcoxon one-sample test. Quantitative analyses were performed between the 2 groups with cerebral blood flow values (ASL-CBF), relative cerebral blood flow values (ASL-rCBF, DSC-rCBF), and relative cerebral blood volume values (DSC-rCBV) using Wilcoxon one-sample test. The intra-class correlation coefficient (ICC) statistics were calculated for testing intrareader variability in regions of interest (ROIs) measurement of all perfusion parameters. RESULTS: The imaging-quality score of 3D-pcASL was higher than that of DSC-MRI (P = .01). The perfusion parameters between tumor recurrence group and treatment effects group had statistically significant differences. There was a significant correlation between ASL-rCBF and DSC-rCBF values (r = 0.803), between ASL-rCBF and DSC-rCBV values (r = 0.763), and between DSC-rCBF and DSC-rCBV (r = 0.907). A receiver operating characteristic (ROC) curve analysis was performed for significant results of perfusion parameters between the 2 groups. Using a cutoff value of 1.110, ASL-rCBF showed the maximum area under the ROC curve (AUC). However, there were no significant differences among different AUCs. The ICC demonstrated excellent agreement for ROIs measurements of ASL-CBF (ICC = 0.9636), dynamic susceptibility contrast- cerebral blood flow (DSC-CBF) (ICC = 0.8508), and dynamic susceptibility contrast-cerebral blood volume (DSC-CBV) (ICC = 0.8543). CONCLUSION: 3D-pcASL is an alternative perfusion method to DSC-MRI for the differentiation between tumor recurrence and treatment effects in gliomas. 3D-pcASL is noninvasive and shows fewer susceptibility artifacts than DSC-MRI.
[Mh] Termos MeSH primário: Neoplasias Encefálicas/diagnóstico por imagem
Neoplasias Encefálicas/patologia
Glioma/diagnóstico por imagem
Glioma/patologia
Angiografia por Ressonância Magnética/métodos
Recidiva Local de Neoplasia/diagnóstico por imagem
Recidiva Local de Neoplasia/patologia
[Mh] Termos MeSH secundário: Artefatos
Neoplasias Encefálicas/cirurgia
Circulação Cerebrovascular
Meios de Contraste
Feminino
Glioma/cirurgia
Seres Humanos
Masculino
Meia-Idade
Estudos Prospectivos
Marcadores de Spin
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Contrast Media); 0 (Spin Labels)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009332


  2 / 8930 MEDLINE  
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[PMID]:29340383
[Au] Autor:Syryamina VN; De Zotti M; Toniolo C; Formaggio F; Dzuba SA
[Ad] Endereço:Institute of Chemical Kinetics and Combustion, RAS, Novosibirsk 630090, Russian Federation. dzuba@kinetics.nsc.ru.
[Ti] Título:Alamethicin self-assembling in lipid membranes: concentration dependence from pulsed EPR of spin labels.
[So] Source:Phys Chem Chem Phys;20(5):3592-3601, 2018 Jan 31.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The antimicrobial action of the peptide antibiotic alamethicin (Alm) is commonly related to peptide self-assembling resulting in the formation of voltage-dependent channels in bacterial membranes, which induces ion permeation. To obtain a deeper insight into the mechanism of channel formation, it is useful to know the dependence of self-assembling on peptide concentration. With this aim, we studied Alm F50/5 spin-labeled analogs in a model 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane, for peptide-to-lipid (P/L) ratios varying between 1/1500 and 1/100. Pulsed electron-electron double resonance (PELDOR) spectroscopy reveals that even at the lowest concentration investigated, the Alm molecules assemble into dimers. Moreover, under these conditions, electron spin echo envelope modulation (ESEEM) spectroscopy of D O-hydrated membranes shows an abrupt change from the in-plane to the trans-membrane orientation of the peptide. Therefore, we hypothesize that dimer formation and peptide reorientation are concurrent processes and represent the initial step of peptide self-assembling. By increasing peptide concentration, higher oligomers are formed. A simple kinetic model of equilibrium among monomers, dimers, and pentamers allows for satisfactorily describing the experimental PELDOR data. The inter-label distances in the oligomers obtained from PELDOR experiments become better resolved with increasing P/L ratio, thus suggesting that the supramolecular organization of the higher-order oligomers becomes more defined.
[Mh] Termos MeSH primário: Alameticina/química
Bicamadas Lipídicas/química
[Mh] Termos MeSH secundário: Alameticina/metabolismo
Sequência de Aminoácidos
Dimerização
Espectroscopia de Ressonância de Spin Eletrônica
Cinética
Bicamadas Lipídicas/metabolismo
Fosfatidilcolinas/química
Marcadores de Spin
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipid Bilayers); 0 (Phosphatidylcholines); 0 (Spin Labels); 059QF0KO0R (Water); 27061-78-5 (Alamethicin); TE895536Y5 (1-palmitoyl-2-oleoylphosphatidylcholine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp07298h


  3 / 8930 MEDLINE  
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[PMID]:29351320
[Au] Autor:Kumar P; van Son M; Zheng T; Valdink D; Raap J; Kros A; Huber M
[Ad] Endereço:Department of Physics, Huygens-Kamerlingh Onnes Laboratory, Leiden University, Leiden, The Netherlands.
[Ti] Título:Coiled-coil formation of the membrane-fusion K/E peptides viewed by electron paramagnetic resonance.
[So] Source:PLoS One;13(1):e0191197, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The interaction of the complementary K (Ac-(KIAALKE)3-GW-NH2) and E (Ac-(EIAALEK)3-GY-NH2) peptides, components of the zipper of an artificial membrane fusion system (Robson Marsden H. et al. Angew Chemie Int Ed. 2009) is investigated by electron paramagnetic resonance (EPR). By frozen solution continuous-wave EPR and double electron-electron resonance (DEER), the distance between spin labels attached to the K- and to the E-peptide is measured. Three constructs of spin-labelled K- and E-peptides are used in five combinations for low temperature investigations. The K/E heterodimers are found to be parallel, in agreement with previous studies. Also, K homodimers in parallel orientation were observed, a finding that was not reported before. Comparison to room-temperature, solution EPR shows that the latter method is less specific to detect this peptide-peptide interaction. Combining frozen solution cw-EPR for short distances (1.8 nm to 2.0 nm) and DEER for longer distances thus proves versatile to detect the zipper interaction in membrane fusion. As the methodology can be applied to membrane samples, the approach presented suggests itself for in-situ studies of the complete membrane fusion process, opening up new avenues for the study of membrane fusion.
[Mh] Termos MeSH primário: Proteínas de Fusão de Membrana/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Simulação por Computador
Espectroscopia de Ressonância de Spin Eletrônica
Fusão de Membrana/fisiologia
Proteínas de Fusão de Membrana/fisiologia
Modelos Moleculares
Oligopeptídeos/química
Domínios e Motivos de Interação entre Proteínas
Estrutura Quaternária de Proteína
Estrutura Secundária de Proteína
Marcadores de Spin
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Membrane Fusion Proteins); 0 (Oligopeptides); 0 (Spin Labels)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191197


  4 / 8930 MEDLINE  
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[PMID]:28740983
[Au] Autor:Consentius P; Gohlke U; Loll B; Alings C; Heinemann U; Wahl MC; Risse T
[Ad] Endereço:Freie Universität Berlin, Institute of Chemistry and Biochemistry, Takustr. 3, 14195 Berlin, Germany. risse@chemie.fu-berlin.de.
[Ti] Título:Combining EPR spectroscopy and X-ray crystallography to elucidate the structure and dynamics of conformationally constrained spin labels in T4 lysozyme single crystals.
[So] Source:Phys Chem Chem Phys;19(31):20723-20734, 2017 Aug 09.
[Is] ISSN:1463-9084
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling is used to investigate the structure and dynamics of conformationally constrained spin labels in T4 lysozyme single crystals. Within a single crystal, the oriented ensemble of spin bearing moieties results in a strong angle dependence of the EPR spectra. A quantitative description of the EPR spectra requires the determination of the unit cell orientation with respect to the sample tube and the orientation of the spin bearing moieties within the crystal lattice. Angle dependent EPR spectra were analyzed by line shape simulations using the stochastic Liouville equation approach developed by Freed and co-workers and an effective Hamiltonian approach. The gain in spectral information obtained from the EPR spectra of single crystalline samples taken at different frequencies, namely the X-band and Q-band, allows us to discriminate between motional models describing the spectra of isotropic solutions similarly well. In addition, it is shown that the angle dependent single crystal spectra allow us to identify two spin label rotamers with very similar side chain dynamics. These results demonstrate the utility of single crystal EPR spectroscopy in combination with spectral line shape simulation techniques to extract valuable dynamic information not readily available from the analysis of isotropic systems. In addition, it will be shown that the loss of electron density in high resolution diffraction experiments at room temperature does not allow us to conclude that there is significant structural disorder in the system.
[Mh] Termos MeSH primário: Bacteriófago T4/enzimologia
Muramidase/química
Proteínas Virais/química
[Mh] Termos MeSH secundário: Cristalografia por Raios X
Espectroscopia de Ressonância de Spin Eletrônica
Ligações de Hidrogênio
Estrutura Terciária de Proteína
Marcadores de Spin
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Spin Labels); 0 (Viral Proteins); EC 3.2.1.17 (Muramidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1039/c7cp03144k


  5 / 8930 MEDLINE  
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[PMID]:29179205
[Au] Autor:Liu H; Jing X; Dong A; Bai B; Wang H
[Ad] Endereço:Department of Cardiology, Tangdu Hospital, the Fourth Military University, Xi'an, China.
[Ti] Título:Overexpression of TIMP3 Protects Against Cardiac Ischemia/Reperfusion Injury by Inhibiting Myocardial Apoptosis Through ROS/Mapks Pathway.
[So] Source:Cell Physiol Biochem;44(3):1011-1023, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: Myocardial ischemia/reperfusion (I/R) injury remains a great challenge in clinical therapy. Tissue inhibitor of metalloproteinases 3 (TIMP3) plays a crucial role in heart physiological and pathophysiological processes. However, the effects of TIMP3 on I/R injury remain unknown. METHODS: C57BL/6 mice were infected with TIMP3 adenovirus by local delivery in myocardium followed by I/R operation or doxorubicin treatment. Neonatal rat cardiomyocytes were pretreated with TIMP3 adenovirus prior to anoxia/reoxygenation (A/R) treatment in vitro. Histology, echocardiography, in vivo phenotypical analysis, flow cytometry and western blotting were used to investigate the altered cardiac function and underlying mechanisms. RESULTS: The results showed that upregulation of TIMP3 in myocardium markedly inhibited myocardial infarct areas and the cardiac dysfunction induced by I/R or by doxorubicin treatment. TUNEL staining revealed that TIMP3 overexpression attenuated I/R-induced myocardial apoptosis, accompanied by decreased Bax/Bcl-2 ratio, Cleaved Caspase-3 and Cleaved Caspase-9 expression. In vitro, A/R-induced cardiomyocyte apoptosis was abrogated by pharmacological inhibition of reactive oxygen species (ROS) production or MAPKs signaling. Attenuation of ROS production reversed A/R-induced MAPKs activation, whereas MAPKs inhibitors showed on effect on ROS production. Furthermore, in vivo or in vitro overexpression of TIMP3 significantly inhibited I/R- or A/R-induced ROS production and MAPKs activation. CONCLUSION: Our findings demonstrate that TIMP3 upregulation protects against cardiac I/R injury through inhibiting myocardial apoptosis. The mechanism may be related to inhibition of ROS-initiated MAPKs pathway. This study suggests that TIMP3 may be a potential therapeutic target for the treatment of I/R injury.
[Mh] Termos MeSH primário: Miocárdio/metabolismo
Inibidor Tecidual de Metaloproteinase-3/metabolismo
[Mh] Termos MeSH secundário: Acetilcisteína/farmacologia
Animais
Antracenos/farmacologia
Apoptose/efeitos dos fármacos
Caspase 3/metabolismo
Caspase 9/metabolismo
Células Cultivadas
Óxidos N-Cíclicos/farmacologia
Doxorrubicina/toxicidade
Ecocardiografia
Coração/diagnóstico por imagem
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Proteínas Quinases Ativadas por Mitógeno/antagonistas & inibidores
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Traumatismo por Reperfusão Miocárdica/induzido quimicamente
Traumatismo por Reperfusão Miocárdica/metabolismo
Traumatismo por Reperfusão Miocárdica/patologia
Miócitos Cardíacos/citologia
Miócitos Cardíacos/efeitos dos fármacos
Miócitos Cardíacos/metabolismo
Fosforilação/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Ratos
Ratos Sprague-Dawley
Espécies Reativas de Oxigênio/metabolismo
Transdução de Sinais/efeitos dos fármacos
Marcadores de Spin
Inibidor Tecidual de Metaloproteinase-3/genética
Proteína X Associada a bcl-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthracenes); 0 (Cyclic N-Oxides); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (Reactive Oxygen Species); 0 (Spin Labels); 0 (Tissue Inhibitor of Metalloproteinase-3); 0 (bcl-2-Associated X Protein); 1TW30Y2766 (pyrazolanthrone); 80168379AG (Doxorubicin); EC 2.7.11.24 (Mitogen-Activated Protein Kinases); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 9); U78ZX2F65X (tempol); WYQ7N0BPYC (Acetylcysteine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180118
[Lr] Data última revisão:
180118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1159/000485401


  6 / 8930 MEDLINE  
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[PMID]:29183754
[Au] Autor:Santos GB; Ribeiro ACG; Lima SNP; Trostchansky A; Cerdeira CD; Brigagão MRPL
[Ad] Endereço:Departamento de Bioquímica, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Alfenas, MG, Brazil.
[Ti] Título:Nitroxide Tempol down-regulates kinase activities associated with NADPH oxidase function in phagocytic cells and potentially decreases their fungicidal response.
[So] Source:Chem Biol Interact;279:203-209, 2018 Jan 05.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:AIMS: The identification of novel targets to control inflammation in humans is probably the primary challenge that impairs the development of new anti-inflammatory drugs. Therefore, the modulation of intracellular signaling pathways in phagocytes may be an interesting means of achieving this goal. However, this change to signaling can compromise the host's susceptibility to invading pathogens. We investigated whether the antioxidant nitroxide Tempol regulates the activity of kinases associated with the production of oxidants in neutrophils, which affects the fungicidal capability of these cells. MAIN METHODS: The effects of Tempol on PMA- or fMLP-activated neutrophils were examined by oxygen consumption as an index of the oxidative burst, a release of extracellular and total Reactive Oxygen Species (ROS) by chemiluminescence, kinase activities through analysis of ATP consumption during enzyme activities and the dot blot immunoassay and, finally, by neutrophil capacity of killing Candida albicans. KEY FINDINGS: Tempol significantly inhibited the neutrophil oxidative burst in a concentration-dependent manner and decreased oxygen consumption (IC50 = 45 µM) and extracellular/total ROS formation with an increase on the lag period response. In addition, Tempol inhibited neutrophil kinase activities (i.e., a decrease in protein phosphorylation) elicited through different biochemical pathways and consequently impaired the fungicidal activity of these cells. SIGNIFICANCE: Although Tempol has potential anti-inflammatory activity that acts on different intracellular pathways (such as those involving kinases), researchers should be cautious, since this nitroxide down-regulated oxidants production and the fungicidal response of neutrophils.
[Mh] Termos MeSH primário: Candida albicans/fisiologia
Óxidos N-Cíclicos/farmacologia
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
NADPH Oxidases/metabolismo
Fagócitos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Óxidos N-Cíclicos/química
Regulação para Baixo/efeitos dos fármacos
Inflamação
Masculino
Camundongos
Estrutura Molecular
Neutrófilos/enzimologia
Consumo de Oxigênio
Fosfotransferases/genética
Fosfotransferases/metabolismo
Marcadores de Spin
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclic N-Oxides); 0 (Spin Labels); EC 1.6.3.- (NADPH Oxidases); EC 2.7.- (Phosphotransferases); U78ZX2F65X (tempol)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE


  7 / 8930 MEDLINE  
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[PMID]:29183043
[Au] Autor:Kamiya T; Nagaoka T; Omae T; Ono S; Otani S; Yoshida A
[Ad] Endereço:Department of Ophthalmology, Asahikawa Medical University, Asahikawa, Japan.
[Ti] Título:Benzo(e)pyrene Inhibits Endothelium-Dependent NO-Mediated Dilation of Retinal Arterioles via Superoxide Production and Endoplasmic Reticulum Stress.
[So] Source:Invest Ophthalmol Vis Sci;58(13):5978-5984, 2017 Nov 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: To investigate whether benzo(e)pyrene (B(e)P), a toxicant in cigarette smoke, affects the endothelium-dependent nitric oxide (NO)-induced vasodilation of the retinal arterioles, and whether oxidative stress, distinct protein kinase signaling pathways, and endoplasmic reticulum (ER) stress are associated with the B(e)P-induced effect on the retinal arterioles. Methods: In this in vitro study, porcine retinal arterioles were isolated, cannulated, and pressurized without flow. These vessels were treated with intraluminal administration of B(e)P or B(e)P plus blockers for 180 minutes. Diametric changes to agonists were recorded by videomicroscopy. Results: Intraluminal treatment with 100 µM B(e)P for 180 minutes significantly reduced the arteriolar vasodilation caused by the endothelium-dependent NO-mediated agonists bradykinin and A23187 but not that caused by endothelium-independent NO donor sodium nitroprusside. The adverse effects of B(e)P on the vasodilatory action of bradykinin were prevented by the superoxide scavenger 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL), the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase) inhibitor apocynin, the c-Jun N-terminal kinase (JNK) inhibitor SP600125, the p38 mitogen-activated protein kinase inhibitor SB203580, genistein, resveratrol (RSV), and the ER stress inhibitor 4-phenylbutyrate (4-PBA). The xanthine oxidase inhibitor allopurinol did not alter the effect of B(e)P on the vasodilatory action induced by bradykinin. Conclusions: B(e)P decreases the endothelium-dependent NO-induced vasodilation in the retinal arterioles through the production of superoxide from NADPH oxidase, which is linked to JNK and p38 kinase. The results suggested that ER stress is instrumental in B(e)P-induced endothelial dysfunction and that genistein and RSV might preserve endothelial function.
[Mh] Termos MeSH primário: Arteríolas/efeitos dos fármacos
Benzopirenos/farmacologia
Estresse do Retículo Endoplasmático/fisiologia
Vasos Retinianos/efeitos dos fármacos
Superóxidos/metabolismo
Vasodilatação/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antioxidantes/farmacologia
Bradicinina/farmacologia
Óxidos N-Cíclicos/farmacologia
Modelos Animais de Doenças
Marcadores de Spin
Suínos
Vasodilatadores/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Benzopyrenes); 0 (Cyclic N-Oxides); 0 (Spin Labels); 0 (Vasodilator Agents); 11062-77-4 (Superoxides); 63APT6398R (benzo(e)pyrene); S8TIM42R2W (Bradykinin); U78ZX2F65X (tempol)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-21925


  8 / 8930 MEDLINE  
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[PMID]:29095282
[Au] Autor:Ryu KH; Baek HJ; Cho SB; Moon JI; Choi BH; Park SE; An HJ
[Ad] Endereço:aDepartment of Radiology bDepartment of Pathology, Gyeongsang National University School of Medicine and Gyeongsang National University Changwon Hospital, Changwon, Republic of Korea.
[Ti] Título:Skull metastases detecting on arterial spin labeling perfusion: Three case reports and review of literature.
[So] Source:Medicine (Baltimore);96(44):e8432, 2017 Nov.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Detection of skull metastases is as important as detection of brain metastases because early diagnosis of skull metastases is a crucial determinant of treatment. However, the skull can be a blind spot for assessing metastases on routine brain magnetic resonance imaging (MRI). To the best of our knowledge, the finding of skull metastases on arterial spin labeling (ASL) has not been reported. ASL is a specific MRI sequence for evaluating cerebral blood flow using magnetized endogenous inflow blood. This study uses ASL as a routine sequence of brain MRI protocol and describes 3 clinical cases of skull metastases identified by ASL. The study also highlights the clinical usefulness of ASL in detecting skull metastases. PATIENT CONCERNS: Three patients with known malignancy underwent brain MRI to evaluate for brain metastases. DIAGNOSES: All of the skull metastases were conspicuously depicted on routine ASL images, and the lesions correlated well with other MRI sequences. INTERVENTIONS: Three patients received palliative chemotherapy. OUTCOMES: Three patients are being followed up regularly at the outpatient department. LESSONS: The routine use of ASL may help to detect lesions in blind spots, such as skull metastases, and to facilitate the evaluation of intracranial pathologies without the use of contrast materials in exceptional situations.
[Mh] Termos MeSH primário: Meios de Contraste
Imagem por Ressonância Magnética/métodos
Metástase Neoplásica/diagnóstico por imagem
Imagem de Perfusão/métodos
Neoplasias da Base do Crânio/diagnóstico por imagem
Marcadores de Spin
[Mh] Termos MeSH secundário: Idoso
Artérias Cerebrais/diagnóstico por imagem
Circulação Cerebrovascular
Feminino
Seres Humanos
Masculino
Meia-Idade
Crânio/diagnóstico por imagem
Neoplasias da Base do Crânio/secundário
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Contrast Media); 0 (Spin Labels)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171103
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008432


  9 / 8930 MEDLINE  
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[PMID]:29019892
[Au] Autor:Kang JH; Yun TJ; Yoo RE; Yoon BW; Lee AL; Kang KM; Choi SH; Kim JH; Sohn CH; Han MH
[Ad] Endereço:aInstitute of Radiation Medicine, Seoul National University Medical Research Center bDepartment of Radiology cClinical Research Center for Stroke, Clinical Research Institute dDepartment of Neurology, Seoul National University Hospital, Seoul eDepartment of Radiology, Soonchunhyang University Bucheon Hospital, Gyunggi-do, Republic of Korea.
[Ti] Título:Bright sinus appearance on arterial spin labeling MR imaging aids to identify cerebral venous thrombosis.
[So] Source:Medicine (Baltimore);96(41):e8244, 2017 Oct.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cerebral venous thrombosis is a potentially lethal disease. Early diagnosis is essential to improve its prognosis. However, its early diagnosis based on conventional imaging modalities remains a challenge in clinical settings. The purpose of this study was to evaluate whether bright sinus appearance on arterial spin-labeling perfusion-weighted image (ASL-PWI) could help identify cerebral venous thrombosis.ASL-PWI of 13 patients who were confirmed as cerebral venous thrombosis based on neurologic symptoms and computed tomography (CT) or magnetic resonance (MR) venography (with/without cerebral angiography) were retrospectively analyzed for the presence or absence of the following: bright signal in dural sinus termed "bright sinus appearance"; and hypoperfusion in brain parenchyma drained by thrombosed sinus. In addition, conventional MR findings, including susceptibility vessel sign, empty delta sign, and atypical distribution against arterial territory, were also analyzed.Bright sinus appearance on ASL-PWI was found in all (100%) 13 patients. In addition, 10 (77%) patients showed hypoperfusion in the brain parenchyma drained by thrombosed sinus on ASL-PWI. Susceptibility vessel sign and empty delta sign were revealed in 11 (85%) and 7 (54%) patients, respectively. Atypical distribution against arterial territory was seen in 5 (50%) of the 10 patients with parenchymal abnormality on conventional MR sequences. Therefore, the bright sinus appearance had higher sensitivities for identifying cerebral venous thrombosis than the susceptibility vessel sign, empty delta sign, and atypical distribution against arterial territory (with differences of 15%; P = .500, 46%; P = .031, and 50%; P = .031, respectively).Bright sinus appearance on ASL-PWI can provide important diagnostic clue for identifying cerebral venous thrombosis. Therefore, this technique may have the potential to be used as a noninvasive diagnostic tool to identify the cerebral venous thrombosis.
[Mh] Termos MeSH primário: Arteriopatias Oclusivas/diagnóstico
Artérias Cerebrais/diagnóstico por imagem
Veias Cerebrais
Trombose Intracraniana
Imagem por Ressonância Magnética/métodos
Trombose Venosa/diagnóstico
[Mh] Termos MeSH secundário: Angiografia Cerebral/métodos
Veias Cerebrais/diagnóstico por imagem
Veias Cerebrais/patologia
Diagnóstico Diferencial
Feminino
Seres Humanos
Trombose Intracraniana/diagnóstico por imagem
Trombose Intracraniana/patologia
Masculino
Meia-Idade
Reprodutibilidade dos Testes
República da Coreia
Estudos Retrospectivos
Marcadores de Spin
[Pt] Tipo de publicação:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Nm] Nome de substância:
0 (Spin Labels)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171122
[Lr] Data última revisão:
171122
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008244


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[PMID]:28837695
[Au] Autor:Chen HJ; Wright GA
[Ad] Endereço:Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.
[Ti] Título:A physiological model for interpretation of arterial spin labeling reactive hyperemia of calf muscles.
[So] Source:PLoS One;12(8):e0183259, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To characterize and interpret arterial spin labeling (ASL) reactive hyperemia of calf muscles for a better understanding of the microcirculation in peripheral arterial disease (PAD), we present a physiological model incorporating oxygen transport, tissue metabolism, and vascular regulation mechanisms. The model demonstrated distinct effects between arterial stenoses and microvascular dysfunction on reactive hyperemia, and indicated a higher sensitivity of 2-minute thigh cuffing to microvascular dysfunction than 5-minute cuffing. The recorded perfusion responses in PAD patients (n = 9) were better differentiated from the normal subjects (n = 7) using the model-based analysis rather than characterization using the apparent peak and time-to-peak of the responses. The analysis results suggested different amounts of microvascular disease within the patient group. Overall, this work demonstrates a novel analysis method and facilitates understanding of the physiology involved in ASL reactive hyperemia. ASL reactive hyperemia with model-based analysis may be used as a noninvasive microvascular assessment in the presence of arterial stenoses, allowing us to look beyond the macrovascular disease in PAD. A subgroup who will have a poor prognosis after revascularization in the patients with critical limb ischemia may be associated with more severe microvascular diseases, which may potentially be identified using ASL reactive hyperemia.
[Mh] Termos MeSH primário: Artérias/fisiopatologia
Hiperemia/fisiopatologia
Músculo Esquelético/fisiopatologia
[Mh] Termos MeSH secundário: Adulto
Estudos de Casos e Controles
Seres Humanos
Modelos Teóricos
Músculo Esquelético/irrigação sanguínea
Doença Arterial Periférica/fisiopatologia
Marcadores de Spin
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Spin Labels)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170825
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183259



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