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[PMID]:28467350
[Au] Autor:Santos HFD; Campos JF; Santos CMD; Balestieri JBP; Silva DB; Carollo CA; de Picoli Souza K; Estevinho LM; Dos Santos EL
[Ad] Endereço:Research group on Biotechnology and Bioprospecting Applied to Metabolism (GEBBAM), Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79804-970 Dourados, MS, Brazil. helderspk@gmail.com.
[Ti] Título:Chemical Profile and Antioxidant, Anti-Inflammatory, Antimutagenic and Antimicrobial Activities of Geopropolis from the Stingless Bee Melipona orbignyi.
[So] Source:Int J Mol Sci;18(5), 2017 May 03.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Geopropolis is a resin mixed with mud, produced only by stingless bees. Despite being popularly known for its medicinal properties, few scientific studies have proven its biological activities. In this context, the objective of this study was to determine the chemical composition and antioxidant, anti-inflammatory, antimutagenic and antimicrobial activities of the geopropolis. The hydroalcoholic extract of geopropolis (HEGP) was prepared and its chemical composition determined by high performance liquid chromatography coupled to diode array detector and mass spectrometry (HPLC-DAD-MS). The antioxidant activity was determined by the capture of free radicals and inhibition of lipid peroxidation in human erythrocytes. The anti-inflammatory activity was evaluated by the inhibition of the hyaluronidase enzyme and the antimutagenic action was investigated in colonies. The antimicrobial activities were determined against bacteria and yeasts, isolated from reference strains and hospital origin. The chemical composition of HEGP included flavonoids, derivatives of glycosylated phenolic acids and terpenoids. HEGP showed high antioxidant activity, it inhibited the activity of the inflammatory enzyme hyaluronidase and reduced the mutagenic effects in . In relation to the antimicrobial activity, it promoted the death of all microorganisms evaluated. In conclusion, this study reveals for the first time the chemical composition of the HEGP of and demonstrates its pharmacological properties.
[Mh] Termos MeSH primário: Anti-Infecciosos
Anti-Inflamatórios
Antioxidantes
Abelhas/química
Própole
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/química
Anti-Infecciosos/farmacologia
Anti-Inflamatórios/química
Anti-Inflamatórios/farmacologia
Antioxidantes/química
Antioxidantes/farmacologia
Bactérias/efeitos dos fármacos
Cromatografia Líquida de Alta Pressão
Eritrócitos/efeitos dos fármacos
Metanossulfonato de Etila/farmacologia
Flavonoides/análise
Radicais Livres/análise
Seres Humanos
Hialuronoglucosaminidase/efeitos dos fármacos
Hidroxibenzoatos/análise
Peroxidação de Lipídeos/efeitos dos fármacos
Espectrometria de Massas
Mutagênicos
Própole/química
Própole/farmacologia
Saccharomyces cerevisiae/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Flavonoids); 0 (Free Radicals); 0 (Hydroxybenzoates); 0 (Mutagens); 29656-58-4 (phenolic acid); 9009-62-5 (Propolis); 9H154DI0UP (Ethyl Methanesulfonate); EC 3.2.1.35 (Hyaluronoglucosaminidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


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[PMID]:29317207
[Au] Autor:Imamura T; Takagi H; Miyazato A; Ohki S; Mizukoshi H; Mori M
[Ad] Endereço:Ishikawa Prefectural University, Nonoichi, Ishikawa, 921-8836, Japan. Electronic address: timamura@ishikawa-up.ac.jp.
[Ti] Título:Isolation and characterization of the betalain biosynthesis gene involved in hypocotyl pigmentation of the allotetraploid Chenopodium quinoa.
[So] Source:Biochem Biophys Res Commun;496(2):280-286, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In quinoa seedlings, the pigment betalain accumulates in the hypocotyl. To isolate the genes involved in betalain biosynthesis in the hypocotyl, we performed ethyl methanesulfonate (EMS) mutagenesis on the CQ127 variety of quinoa seedlings. While putative amaranthin and celosianin II primarily accumulate in the hypocotyls, this process produced a green hypocotyl mutant (ghy). This MutMap+ method using the quinoa draft genome revealed that the causative gene of the mutant is CqCYP76AD1-1. Our results indicated that the expression of CqCYP76AD1-1 was light-dependent. In addition, the transient expression of CqCYP76AD1-1 in Nicotiana benthamiana leaves resulted in the accumulation of betanin but not isobetanin, and the presence of a polymorphism in CqCYP76A1-2 in the CQ127 variety was shown to have resulted in its loss of function. These findings suggested that CqCYP76AD1-1 is involved in betalain biosynthesis during the hypocotyl pigmentation process in quinoa. To our knowledge, CqCYP76AD1-1 is the first quinoa gene identified by EMS mutagenesis using a draft gene sequence.
[Mh] Termos MeSH primário: O-Dealquilase 7-Alcoxicumarina/genética
Betalaínas/biossíntese
Chenopodium quinoa/genética
Regulação da Expressão Gênica de Plantas
Genes de Plantas
Hipocótilo/genética
[Mh] Termos MeSH secundário: O-Dealquilase 7-Alcoxicumarina/metabolismo
Sequência de Bases
Betacianinas/biossíntese
Chenopodium quinoa/efeitos dos fármacos
Chenopodium quinoa/crescimento & desenvolvimento
Chenopodium quinoa/metabolismo
Metanossulfonato de Etila/farmacologia
Hipocótilo/efeitos dos fármacos
Hipocótilo/crescimento & desenvolvimento
Hipocótilo/metabolismo
Luz
Mutagênese
Mutagênicos/farmacologia
Pigmentação
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/genética
Folhas de Planta/crescimento & desenvolvimento
Folhas de Planta/metabolismo
Polimorfismo Genético
Tabaco/genética
Tabaco/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Betacyanins); 0 (Mutagens); 15167-84-7 (amaranthin betacyanin); 37279-84-8 (Betalains); 5YJC992ZP6 (betanin); 9H154DI0UP (Ethyl Methanesulfonate); EC 1.14.13.- (7-Alkoxycoumarin O-Dealkylase); EC 1.14.13.- (cytochrome P-450 CYP76B1 (Helianthus tuberosus))
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE


  3 / 1879 MEDLINE  
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[PMID]:28493864
[Au] Autor:Plech M; Tomala K; Tutaj H; Piwcewicz DE; de Visser JAGM; Korona R
[Ad] Endereço:Institute of Environmental Sciences, Jagiellonian University, Krakow, Poland.
[Ti] Título:Power provides protection: Genetic robustness in yeast depends on the capacity to generate energy.
[So] Source:PLoS Genet;13(5):e1006768, 2017 May.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The functional basis of genetic robustness, the ability of organisms to suppress the effects of mutations, remains incompletely understood. We exposed a set of 15 strains of Saccharomyces cerevisiae form diverse environments to increasing doses of the chemical mutagen EMS. The number of the resulting random mutations was similar for all tested strains. However, there were differences in immediate mortality after the mutagenic treatment and in defective growth of survivors. An analysis of gene expression revealed that immediate mortality was lowest in strains with lowest expression of transmembrane proteins, which are rich in thiol groups and thus vulnerable to EMS. A signal of genuine genetic robustness was detected for the other trait, the ability to grow well despite bearing non-lethal mutations. Increased tolerance of such mutations correlated with high expression of genes responsible for the oxidative energy metabolism, suggesting that the negative effect of mutations can be buffered if enough energy is available. We confirmed this finding in three additional tests of the ability to grow on (i) fermentable or non-fermentable sources of carbon, (ii) under chemical inhibition of the electron transport chain and (iii) during overexpression of its key component, cytochrome c. Our results add the capacity to generate energy as a general mechanism of genetic robustness.
[Mh] Termos MeSH primário: Citocromos c/genética
Metabolismo Energético/genética
Interação Gene-Ambiente
Saccharomyces cerevisiae/genética
[Mh] Termos MeSH secundário: Citocromos c/biossíntese
Metanossulfonato de Etila/toxicidade
Regulação Fúngica da Expressão Gênica/efeitos dos fármacos
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/genética
Mitocôndrias/metabolismo
Mutagênese/efeitos dos fármacos
Mutação/genética
Fosforilação Oxidativa/efeitos dos fármacos
Saccharomyces cerevisiae/crescimento & desenvolvimento
Saccharomyces cerevisiae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-43-6 (Cytochromes c); 9H154DI0UP (Ethyl Methanesulfonate)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170607
[Lr] Data última revisão:
170607
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006768


  4 / 1879 MEDLINE  
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[PMID]:28304234
[Au] Autor:Carmona ER; Reyes-Díaz M; Parodi J; Inostroza-Blancheteau C
[Ad] Endereço:a Núcleo de Investigación en Bioproductos y Materiales Avanzados (BioMA), Facultad de Ingeniería , Universidad Católica de Temuco , Temuco , Chile.
[Ti] Título:Antimutagenic evaluation of traditional medicinal plants from South America Peumus boldus and Cryptocarya alba using Drosophila melanogaster.
[So] Source:J Toxicol Environ Health A;80(4):208-217, 2017.
[Is] ISSN:1528-7394
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Peumus boldus Mol. ("Boldo") and Cryptocarya alba Mol. Looser ("Peumo") are medicinal shrubs with wide geographical distribution in South America. Their leaves and fruits are commonly used in traditional medicine because they exhibit natural medicinal properties for treatment of liver disorders and rheumatism. However, there are no apparent data regarding potential protective effects on cellular genetic components. In order to examine potential mutagenic and/or antimutagenic effects of these medicinal plants, the Drosophila melanogaster (D. melanogaster) wing-spot test was employed. This assay detects a wide range of mutational events, including point mutations, deletions, certain types of chromosomal aberrations (nondisjunction), and mitotic recombination. Qualitative and quantitative analyses of phenolic and anthocyanin compounds were carried out using biochemical and high-performance liquid chromatography methodologies. In addition, the antioxidant capacity of P. boldus and C. alba leaf extracts was also analyzed. P. boldus and C. alba extracts did not induce significant mutagenic effects in the D. melanogaster model. However, simultaneous treatment of extracts concurrently with the mutagen ethyl methane sulphonate showed a decrease of mutant spots in somatic cells of D. melanogaster, indicating desmutagenic effects in this in vivo model. Flavonoids and anthocyanins were detected predominantly in the extracts, and these compounds exerted significant antioxidant capacity. The observed antimutagenic effects may be related to the presence of phytochemicals with high antioxidant capacity, such as flavonoids and antohocyanins, in the extracts.
[Mh] Termos MeSH primário: Antimutagênicos/farmacologia
Cryptocarya/química
Drosophila melanogaster/efeitos dos fármacos
Peumus/química
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Animais
Antocianinas/análise
Antocianinas/farmacologia
Antioxidantes/análise
Antioxidantes/farmacologia
Chile
Drosophila melanogaster/crescimento & desenvolvimento
Metanossulfonato de Etila/metabolismo
Larva/efeitos dos fármacos
Mutagênicos/metabolismo
Fenóis/análise
Fenóis/farmacologia
Extratos Vegetais/química
Folhas de Planta/química
Asas de Animais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antimutagenic Agents); 0 (Antioxidants); 0 (Mutagens); 0 (Phenols); 0 (Plant Extracts); 9H154DI0UP (Ethyl Methanesulfonate)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE
[do] DOI:10.1080/15287394.2017.1279574


  5 / 1879 MEDLINE  
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[PMID]:28196091
[Au] Autor:Rizwan M; Aslam M; Asghar MJ; Abbas G; Shah TM; Shimelis H
[Ad] Endereço:Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan.
[Ti] Título:Pre-breeding of lentil (Lens culinaris Medik.) for herbicide resistance through seed mutagenesis.
[So] Source:PLoS One;12(2):e0171846, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lentil is a poor competitor of weeds and its sensitivity to herbicides is a major hurdle for large scale production. The present study was conducted to select herbicide resistant lentil genotypes through seed mutagenesis. Seeds of three advanced lentil genotypes (LPP 11001, LPP 11100 and LPP 11116) were treated with two different concentrations of ethyl methanesulfonate (EMS; 0.1 and 0.2%), hydrazine hydrate (HH; 0.02 and 0.03%) and sodium azide (SA; 0.01 and 0.02%) to develop M1 seed. The M2 was screened against two herbicides including Ally Max 28.6% SG (X = 34.58 g/ha and 1.5X = 51.87 g/ha) and Atlantis 3.6% WG (X = 395.2 g/ha and 1.5X = 592.8 g/ha) using the following three screening methods: post plant emergence (PPE), pre-plant incorporation (PPI) and seed priming (SP). Data were recorded on survival index and survival percentage from each experimental unit of every population. Plants in all populations were categorized following their reaction to herbicides. The newly developed populations showed greater variation for herbicide resistance when compared to their progenitors. Phenotypic traits were significantly reduced in all the screening environments. Overall, 671 herbicide resistant mutants were selected from all testing environments. The seeds from selected plants were re-mutagenized at 150 Gy of gamma radiation and evaluated against higher dose of herbicides. This allowed selection of 134 herbicide resistant mutants. The selected mutants are useful germplasm for herbicide resistance breeding of lentil.
[Mh] Termos MeSH primário: Resistência a Herbicidas/genética
Lens (Planta)/genética
Plantas Daninhas/genética
Sementes/genética
[Mh] Termos MeSH secundário: Carcinógenos/toxicidade
Metanossulfonato de Etila/toxicidade
Raios gama
Genes de Plantas/genética
Genética Populacional/métodos
Genótipo
Herbicidas/farmacologia
Hidrazinas/toxicidade
Lens (Planta)/efeitos dos fármacos
Lens (Planta)/crescimento & desenvolvimento
Mutagênese/efeitos dos fármacos
Mutagênese/efeitos da radiação
Mutagênicos/toxicidade
Mutação
Fenótipo
Melhoramento Vegetal/métodos
Plantas Daninhas/efeitos dos fármacos
Plantas Daninhas/crescimento & desenvolvimento
Sementes/efeitos dos fármacos
Sementes/crescimento & desenvolvimento
Azida Sódica/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carcinogens); 0 (Herbicides); 0 (Hydrazines); 0 (Mutagens); 27RFH0GB4R (hydrazine); 968JJ8C9DV (Sodium Azide); 9H154DI0UP (Ethyl Methanesulfonate)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170215
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171846


  6 / 1879 MEDLINE  
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[PMID]:28158673
[Au] Autor:Baxley RM; Bullard JD; Klein MW; Fell AG; Morales-Rosado JA; Duan T; Geyer PK
[Ad] Endereço:Interdisciplinary Graduate Program in Molecular and Cellular Biology, University of Iowa, Iowa City, IA 52242, USA.
[Ti] Título:Deciphering the DNA code for the function of the Drosophila polydactyl zinc finger protein Suppressor of Hairy-wing.
[So] Source:Nucleic Acids Res;45(8):4463-4478, 2017 May 05.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Polydactyl zinc finger (ZF) proteins have prominent roles in gene regulation and often execute multiple regulatory functions. To understand how these proteins perform varied regulation, we studiedDrosophila Suppressor of Hairy-wing [Su(Hw)], an exemplar multifunctional polydactyl ZF protein. We identified separation-of-function (SOF) alleles that encode proteins disrupted in a single ZF that retain one of the Su(Hw) regulatory activities. Through extended in vitro analyses of the Su(Hw) ZF domain, we show that clusters of ZFs bind individual modules within a compound DNA consensus sequence. Through in vivo analysis of SOF mutants, we find that Su(Hw) genomic sites separate into sequence subclasses comprised of combinations of modules, with subclasses enriched for different chromatin features. These data suggest a Su(Hw) code, wherein DNA binding dictates its cofactor recruitment and regulatory output. We propose that similar DNA codes might be used to confer multiple regulatory functions of other polydactyl ZF proteins.
[Mh] Termos MeSH primário: Cromatina/química
DNA/genética
Proteínas de Drosophila/genética
Drosophila melanogaster/genética
Proteínas Repressoras/genética
Dedos de Zinco
[Mh] Termos MeSH secundário: Alelos
Animais
Sequência de Bases
Sítios de Ligação
Cromatina/efeitos dos fármacos
Cromatina/metabolismo
DNA/metabolismo
Proteínas de Drosophila/metabolismo
Drosophila melanogaster/efeitos dos fármacos
Drosophila melanogaster/metabolismo
Metanossulfonato de Etila/farmacologia
Feminino
Regulação da Expressão Gênica
Genótipo
Masculino
Mutagênicos/farmacologia
Mutação
Fenótipo
Ligação Proteica
Domínios Proteicos
Proteínas Repressoras/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (Drosophila Proteins); 0 (Mutagens); 0 (Repressor Proteins); 0 (su(Hw) protein, Drosophila); 9007-49-2 (DNA); 9H154DI0UP (Ethyl Methanesulfonate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx040


  7 / 1879 MEDLINE  
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[PMID]:28108073
[Au] Autor:Kasamoto S; Masumori S; Tanaka J; Ueda M; Fukumuro M; Nagai M; Yamate J; Hayashi M
[Ad] Endereço:Public Interest Incorporated Foundation, BioSafety Research Center (BSRC), 582-2, Shioshinden, Iwata, Shizuoka, 437-1213, Japan. Electronic address: kasamoto-sawako@anpyo.or.jp.
[Ti] Título:Reference control data obtained from an in vivo comet-micronucleus combination assay using Sprague Dawley rats.
[So] Source:Exp Toxicol Pathol;69(4):187-191, 2017 Apr 04.
[Is] ISSN:1618-1433
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:According to the International Conference on Harmonization Guidance on Genotoxicity Testing and Data Interpretation for Pharmaceuticals Intended for Human Use (ICH S2(R1)), a positive response in any in vitro assay necessitates additional in vivo test(s) (other tissue/endpoint) in addition to the erythrocyte micronucleus test when Option 1 of the test battery is selected. When Option 2 of the test battery is selected, a bacterial gene mutation test and two in vivo tests with different tissues/endpoint are required. The in vivo alkaline comet assay is recommended as the second in vivo test because it can detect a broad spectrum of DNA damage in any tissue and can be combined with the erythrocyte micronucleus test. Considering animal welfare, a combination assay is preferable to an individual assay. Thus, we validated the protocol for the in vivo comet-micronucleus combination assay in rats with three daily administrations and determined the dose of the positive control (ethyl methanesulfonate; EMS, 200mg/kg/day). We also collected the negative control (vehicle) and positive control (EMS) data from the comet (liver, stomach, and kidney) and micronucleus (bone marrow) combination assay using male Sprague Dawley (SD) rats. The negative control data were comparable to our historical control data obtained from stand-alone assays. The positive control data showed clear and consistent positive responses in both endpoints.
[Mh] Termos MeSH primário: Ensaio Cometa/métodos
Testes para Micronúcleos/métodos
Mutagênicos/toxicidade
[Mh] Termos MeSH secundário: Animais
Grupos Controle
Metanossulfonato de Etila/toxicidade
Masculino
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mutagens); 9H154DI0UP (Ethyl Methanesulfonate)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170122
[St] Status:MEDLINE


  8 / 1879 MEDLINE  
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[PMID]:28079919
[Au] Autor:Alaraby M; Hernández A; Marcos R
[Ad] Endereço:Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Barcelona, Spain.
[Ti] Título:Copper oxide nanoparticles and copper sulphate act as antigenotoxic agents in drosophila melanogaster.
[So] Source:Environ Mol Mutagen;58(1):46-55, 2017 Jan.
[Is] ISSN:1098-2280
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The biological reactivity of metal and metal oxide nanomaterials is attributed to their redox properties, which would explain their pro- or anti-cancer properties depending on exposure circumstances. In this sense, copper oxide nanoparticles (CuONP) have been proposed as a potential anti-tumoral agent. The aim of this study was to assess if CuONP can exert antigenotoxic effects using Drosophila melanogaster as an in vivo model. Genotoxicity was induced by two well-known genotoxic compounds, namely potassium dichromate (PD) and ethyl methanesulfonate (EMS). The wing-spot assay and the comet assay were used as biomarkers of genotoxic effects. In addition, changes in the expression of Ogg1 and Sod genes were determined. The effects of CuONP cotreatment were compared with those induced by copper sulfate (CS), an agent releasing copper ions. Using the wing-spot assay, CuONP and CS were not able to reduce the genotoxic effects of EMS exposure, but had the ability to decrease the effects induced by PD, reducing the frequency of mutant twin-spots that arise from mitotic recombination. In addition, CuONP and CS were able to reduce the DNA damage induced by PD as determined by the comet assay. In general, similar qualitative antigenotoxic effects were obtained with both copper compounds. The antigenotoxic effects of environmentally relevant and non-toxic doses of CuONP and CS may be explained by their ability to partially restore the expression levels of the repair gene Ogg1 and the antioxidant gene Cu,ZnSod, both of which are inhibited by PD treatment. Environ. Mol. Mutagen. 58:46-55, 2017. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Antimutagênicos/farmacologia
Sulfato de Cobre/farmacologia
Cobre/farmacologia
Dano ao DNA/efeitos dos fármacos
Drosophila melanogaster/efeitos dos fármacos
Nanopartículas/química
[Mh] Termos MeSH secundário: Animais
Antimutagênicos/química
Ensaio Cometa
Cobre/química
Sulfato de Cobre/química
DNA Glicosilases/genética
Reparo do DNA/efeitos dos fármacos
Reparo do DNA/genética
Relação Dose-Resposta a Droga
Proteínas de Drosophila/genética
Drosophila melanogaster/genética
Metanossulfonato de Etila/toxicidade
Mutagênicos/toxicidade
Dicromato de Potássio/toxicidade
Reação em Cadeia da Polimerase em Tempo Real
Superóxido Dismutase/genética
Propriedades de Superfície
Asas de Animais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimutagenic Agents); 0 (Drosophila Proteins); 0 (Mutagens); 789U1901C5 (Copper); 9H154DI0UP (Ethyl Methanesulfonate); EC 1.15.1.1 (Superoxide Dismutase); EC 3.2.2.- (DNA Glycosylases); EC 3.2.2.- (Ogg1 protein, Drosophila); LRX7AJ16DT (Copper Sulfate); T4423S18FM (Potassium Dichromate); T8BEA5064F (cuprous oxide)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE
[do] DOI:10.1002/em.22068


  9 / 1879 MEDLINE  
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[PMID]:28060408
[Au] Autor:Lehrbach NJ; Ji F; Sadreyev R
[Ad] Endereço:Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts.
[Ti] Título:Next-Generation Sequencing for Identification of EMS-Induced Mutations in Caenorhabditis elegans.
[So] Source:Curr Protoc Mol Biol;117:7.29.1-7.29.12, 2017 Jan 05.
[Is] ISSN:1934-3647
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Forward genetic analysis using chemical mutagenesis in model organisms is a powerful tool for investigation of molecular mechanisms in biological systems. In the nematode, Caenorhabditis elegans, mutagenesis screens using ethyl methanesulfonate (EMS) have led to important insights into genetic control of animal development and physiology. A major bottleneck to this approach is identification of the causative mutation underlying a phenotype of interest. In the past, this has required time-consuming genetic mapping experiments. More recently, next-generation sequencing technologies have allowed development of new methods for rapid mapping and identification of EMS-induced lesions. In this unit we describe a protocol to map and identify EMS-induced mutations in C. elegans. © 2017 by John Wiley & Sons, Inc.
[Mh] Termos MeSH primário: Caenorhabditis elegans/genética
Metanossulfonato de Etila
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Mutagênese
Mutagênicos
Mutação
[Mh] Termos MeSH secundário: Animais
Caenorhabditis elegans/efeitos dos fármacos
Mapeamento Cromossômico/métodos
DNA/genética
Metanossulfonato de Etila/farmacologia
Mutagênese/efeitos dos fármacos
Mutagênicos/farmacologia
Mutação/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mutagens); 9007-49-2 (DNA); 9H154DI0UP (Ethyl Methanesulfonate)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.1002/cpmb.27


  10 / 1879 MEDLINE  
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[PMID]:27838815
[Au] Autor:Souza PF; Silva FD; Carvalho FE; Silveira JA; Vasconcelos IM; Oliveira JT
[Ad] Endereço:Laboratory of Plant Defense Proteins, Department of Biochemistry and Molecular Biology, Federal University of Ceara, Av. Mister Hull, P.O. Box: 60451, Fortaleza, CE, Brazil.
[Ti] Título:Photosynthetic and biochemical mechanisms of an EMS-mutagenized cowpea associated with its resistance to cowpea severe mosaic virus.
[So] Source:Plant Cell Rep;36(1):219-234, 2017 Jan.
[Is] ISSN:1432-203X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: The seed treatment of a CPSMV-susceptible cowpea genotype with the mutagenic agent EMS generated mutagenized resistant plantlets that respond to the virus challenge by activating biochemical and physiological defense mechanisms. Cowpea is an important crop that makes major nutritional contributions particularly to the diet of the poor population worldwide. However, its production is low, because cowpea is naturally exposed to several abiotic and biotic stresses, including viral agents. Cowpea severe mosaic virus (CPSMV) drastically affects cowpea grain production. This study was conducted to compare photosynthetic and biochemical parameters of a CPSMV-susceptible cowpea (CE-31 genotype) and its derived ethyl methanesulfonate-mutagenized resistant plantlets, both challenged with CPSMV, to shed light on the mechanisms of virus resistance. CPSMV inoculation was done in the fully expanded secondary leaves, 15 days after planting. At 7 days post-inoculation, in vivo photosynthetic parameters were measured and leaves collected for biochemical analysis. CPSMV-inoculated mutagenized-resistant cowpea plantlets (MCPI) maintained higher photosynthesis index, chlorophyll, and carotenoid contents in relation to the susceptible (CE-31) CPSMV-inoculated cowpea (CPI). Visually, the MCPI leaves did not exhibit any viral symptoms neither the presence of the virus as examined by RT-PCR. In addition, MCPI showed higher SOD, GPOX, chitinase, and phenylalanine ammonia lyase activities, H O , phenolic contents, and cell wall lignifications, but lower CAT and APX activities in comparison to CPI. All together, these photosynthetic and biochemical changes might have contributed for the CPSMS resistance of MCPI. Contrarily, CPI plantlets showed CPSMV accumulation, severe disease symptoms, reduction in the photosynthesis-related parameters, chlorophyll, carotenoid, phenolic compound, and H O contents, in addition to increased ß-1,3-glucanase, and catalase activities that might have favored viral infection.
[Mh] Termos MeSH primário: Comovirus/fisiologia
Resistência à Doença
Mutagênese/genética
Fotossíntese
Doenças das Plantas/virologia
Vigna/fisiologia
Vigna/virologia
[Mh] Termos MeSH secundário: Dióxido de Carbono/metabolismo
Carotenoides/metabolismo
Clorofila/metabolismo
Metanossulfonato de Etila
Homeostase
Peróxido de Hidrogênio/metabolismo
Lignina/metabolismo
Oxirredução
Fenóis/metabolismo
Fenilalanina Amônia-Liase/metabolismo
Folhas de Planta/enzimologia
Folhas de Planta/virologia
Proteínas de Plantas/metabolismo
Solubilidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenols); 0 (Plant Proteins); 1406-65-1 (Chlorophyll); 142M471B3J (Carbon Dioxide); 36-88-4 (Carotenoids); 9005-53-2 (Lignin); 9H154DI0UP (Ethyl Methanesulfonate); BBX060AN9V (Hydrogen Peroxide); EC 4.3.1.24 (Phenylalanine Ammonia-Lyase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161114
[St] Status:MEDLINE
[do] DOI:10.1007/s00299-016-2074-z



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