| [PMID]: | 28139350 |
| [Au] Autor: | Kudoh K; Hotta S; Sekine M; Fujii R; Uchida A; Kubota G; Kawano Y; Ihara M |
| [Ad] Endereço: | Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minamiminowa, Nagano 399-4511, Japan. |
| [Ti] Título: | Overexpression of endogenous 1-deoxy-d-xylulose 5-phosphate synthase (DXS) in cyanobacterium Synechocystis sp. PCC6803 accelerates protein aggregation. |
| [So] Source: | J Biosci Bioeng;123(5):590-596, 2017 May. |
| [Is] ISSN: | 1347-4421 |
| [Cp] País de publicação: | Japan |
| [La] Idioma: | eng |
| [Ab] Resumo: | 1-Deoxy-d-xylulose 5-phosphate synthase (DXS) is a rate-limiting enzyme in the 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway, which is responsible for the production of precursors of all isoprenoids. In a previous study, we had examined the overexpression of an endogenous DXS in a Synechocystis sp. PCC6803 mutant (DXS_ox), and found that the dxs mRNA level was 4-fold higher than that in the wild-type (WT) strain. However, the DXS protein level was only 1.5-fold higher, leading to the assumption that the level might be regulated by post-transcriptional events. In this study, we have additionally introduced an exogenous isoprene synthase (IspS; which can release MEP pathway products from the cell as gaseous isoprene) into the WT and DXS_ox strains (WT-isP and DXSox-isP strains, respectively), and their detailed DXS expression profiles were investigated from the induction phase through to the late-logarithmic phase. In the induction phase, the isoprene productivity of the DXSox-isP strain was slightly but significantly (1.4- to 1.8-fold) higher than that of the WT-isP strain, whereas the levels were comparable in the other phases. Interestingly, the ratios of soluble:insoluble DXS protein were remarkably low in the DXSox-isP strain during the induction phase to the early-logarithmic phase, resulting in a moderate level of soluble DXS. All our results suggested that the high translation rate of DXS disturbs the refolding process of DXS. To enhance the concentration of the active DXS in cyanobacteria, the enhancement of the DXS maturation system or the introduction of exogenous and robust DXS proteins might be necessary. |
| [Mh] Termos MeSH primário: |
Agregados Proteicos
Synechocystis/genética
Synechocystis/metabolismo
Transferases/genética
Transferases/metabolismo
|
| [Mh] Termos MeSH secundário: |
Alquil e Aril Transferases/biossíntese
Alquil e Aril Transferases/genética
Alquil e Aril Transferases/metabolismo
Butadienos
Eritritol/análogos & derivados
Eritritol/metabolismo
Gases/metabolismo
Hemiterpenos/biossíntese
Engenharia Metabólica
Pentanos
Pentosefosfatos/biossíntese
RNA Mensageiro/análise
Solubilidade
Fosfatos Açúcares/metabolismo
Terpenos/metabolismo
Transferases/biossíntese
|
| [Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (1-deoxylulose 5-phosphate); 0 (2-C-methylerythritol 4-phosphate); 0 (Butadienes); 0 (Gases); 0 (Hemiterpenes); 0 (Pentanes); 0 (Pentosephosphates); 0 (Protein Aggregates); 0 (RNA, Messenger); 0 (Sugar Phosphates); 0 (Terpenes); 0A62964IBU (isoprene); EC 2.- (Transferases); EC 2.2.1.- (deoxyxylulose-5-phosphate synthase); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.- (isoprene synthase); RA96B954X6 (Erythritol) |
| [Em] Mês de entrada: | 1705 |
| [Cu] Atualização por classe: | 170509 |
[Lr] Data última revisão:
| 170509 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 170201 |
| [St] Status: | MEDLINE |
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