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Pesquisa : D02.455.326.271.665.550.600 [Categoria DeCS]
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[PMID]:29238194
[Au] Autor:Du C; Yan H; Liang J; Luo A; Wang L; Zhu J; Xiong H; Chen Y
[Ad] Endereço:Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine, Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei University, Wuhan.
[Ti] Título:Polyethyleneimine-capped silver nanoclusters for microRNA oligonucleotide delivery and bacterial inhibition.
[So] Source:Int J Nanomedicine;12:8599-8613, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Efficient and safe nonviral gene delivery systems are a prerequisite for the clinical application of therapeutic genes. In this paper, polyethyleneimine-capped silver nanoclusters (PEI-AgNCs) were prepared for the purpose of microRNA (miRNA) delivery. The resultant PEI-AgNCs were characterized by a photoluminescence assay and transmission electron microscopy. A cytotoxicity assay showed that PEI-AgNCs exhibit relatively low cytotoxicity. Interestingly, PEI-AgNCs were confirmed to transfect miRNA mimics more effectively than PEI in HepG2 and 293A cells. In this regard, hsa-miR-21 or hsa-miR-221 mimics (miR-21/221m) were transported into HepG2 cells by using PEI-AgNCs. The miR-21/221 expression was determined post-transfection by quantitative real-time polymerase chain reaction. Compared with the negative control, PEI-AgNCs/miR-21/221m groups exhibited higher miR-21/221 levels. In addition, AgNCs endow PEI with stronger antibacterial activity, and this advantage provided PEI-AgNCs the potential to prevent bacterial contamination during the transfection process. Furthermore, we showed that PEI-AgNCs are viable nanomaterials for plain imaging of the cells by laser scanning confocal microscopy, indicating great potential as an ideal fluorescent probe to track the transfection behavior. These results demonstrated that PEI-AgNCs are promising and novel nonviral vectors for gene delivery.
[Mh] Termos MeSH primário: MicroRNAs/administração & dosagem
Nanoestruturas/química
Polietilenoimina/química
Prata/administração & dosagem
Transfecção/métodos
[Mh] Termos MeSH secundário: Antibacterianos/administração & dosagem
Antibacterianos/farmacologia
Escherichia coli/efeitos dos fármacos
Células HEK293
Células Hep G2
Seres Humanos
MicroRNAs/genética
Microscopia Confocal
Nanoestruturas/administração & dosagem
Oligonucleotídeos/administração & dosagem
Polietilenoimina/farmacologia
Reação em Cadeia da Polimerase em Tempo Real
Prata/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (MIRN21 microRNA, human); 0 (MIRN221 microRNA, human); 0 (MicroRNAs); 0 (Oligonucleotides); 3M4G523W1G (Silver); 9002-98-6 (Polyethyleneimine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S146968


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[PMID]:29428598
[Au] Autor:Kazemi Oskuee R; Dabbaghi M; Gholami L; Taheri-Bojd S; Balali-Mood M; Mousavi SH; Malaekeh-Nikouei B
[Ad] Endereço:Neurogenic inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medical Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: Oskueekr@mums.ac.ir.
[Ti] Título:Investigating the influence of polyplex size on toxicity properties of polyethylenimine mediated gene delivery.
[So] Source:Life Sci;197:101-108, 2018 Mar 15.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: Gene therapy is a promising strategy for the treatment of various diseases. Polyethylenimine (PEI) has received considerable attention for gene delivery applications due to their appropriate properties. However, their toxicity has raised concerns which cause to be used with cautious. This study aimed to prepare different complexes of PEI/DNA and evaluate their parameters affecting in vitro cytotoxicity. Also, apoptosis rate was measured to determine the mechanism of cell toxicity. MATERIALS AND METHODS: The complexes were prepared through conjugation and characterized using dynamic light scattering, MTT and flow cytometry techniques. KEY FINDINGS: The particles' size was from 81 nm to 2785 nm and was increased in the HBS buffer compared to HBG buffer. In the case of branched PEIs, the size of particles was inversely associated with molecular weight. The cytotoxicity results showed that linear 250 KDa PEI was non-toxic whereas branched PEIs with lower molecular weights showed toxicity effects in a concentration dependent manner. Also, the cytotoxicity effects of branched PEIs were proportional with carrier/plasmid (C/P) ratio and were more for the polyplexes prepared in HBG buffer compared to HBS buffer after 24 h incubation. Flow cytometry results confirmed that apoptosis is the main mechanism of cell toxicity produced by polyplexes. SIGNIFICANCE: The results showed the effect of PEI size on its cytotoxicity. Also, the toxicity effects of PEI-derived polyplexes in vivo environment was evaluated.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Técnicas de Transferência de Genes
Teste de Materiais
Polietilenoimina/farmacologia
[Mh] Termos MeSH secundário: Linhagem Celular
Seres Humanos
Tamanho da Partícula
Polietilenoimina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9002-98-6 (Polyethyleneimine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180212
[St] Status:MEDLINE


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[PMID]:29025649
[Au] Autor:Shi Y; Xu D; Liu M; Fu L; Wan Q; Mao L; Dai Y; Wen Y; Zhang X; Wei Y
[Ad] Endereço:Department of Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, PR China; School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, PR China.
[Ti] Título:Room temperature preparation of fluorescent starch nanoparticles from starch-dopamine conjugates and their biological applications.
[So] Source:Mater Sci Eng C Mater Biol Appl;82:204-209, 2018 Jan 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Fluorescent organic nanoparticles (FONs) have been regarded as the promising candidates for biomedical applications owing to their well adjustment of chemical structure and optical properties and good biological properties. However, the preparation of FONs from the natural derived polymers has been rarely reported thus far. In current work, we reported a novel strategy for preparation of FONs based on the self-polymerization of starch-dopamine conjugates and polyethyleneimine in rather mild experimental conditions, including air atmosphere, aqueous solution, absent catalysts and at room temperature. The morphology, chemical structure and optical properties of the resultant starch-based FONs were investigated by different characterization techniques. Biological evaluation results demonstrated that these starch-based FONs possess good biocompatibility and fluorescent imaging performance. More importantly, the novel strategy might also be extended for the preparation of many other carbohydrate polymers based FONs with different structure and functions. Therefore, this work opens a new avenue for the preparation and biomedical applications of luminescent carbohydrate polymers.
[Mh] Termos MeSH primário: Dopamina/química
Nanopartículas/química
Amido/química
[Mh] Termos MeSH secundário: Células A549
Materiais Biocompatíveis/química
Materiais Biocompatíveis/toxicidade
Sobrevivência Celular/efeitos dos fármacos
Seres Humanos
Microscopia Confocal
Microscopia Eletrônica de Transmissão
Nanopartículas/toxicidade
Nanopartículas/ultraestrutura
Polietilenoimina/química
Espectrometria de Fluorescência
Espectroscopia de Infravermelho com Transformada de Fourier
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 9002-98-6 (Polyethyleneimine); 9005-25-8 (Starch); VTD58H1Z2X (Dopamine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


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[PMID]:29172759
[Au] Autor:Wang S; Shao M; Zhong Z; Wang A; Cao J; Lu Y; Wang Y; Zhang J
[Ad] Endereço:a State Key Laboratory of Quality Research in Chinese Medicine , Institute of Chinese Medical Sciences, University of Macau , Macau , China.
[Ti] Título:Co-delivery of gambogic acid and TRAIL plasmid by hyaluronic acid grafted PEI-PLGA nanoparticles for the treatment of triple negative breast cancer.
[So] Source:Drug Deliv;24(1):1791-1800, 2017 Nov.
[Is] ISSN:1521-0464
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-based combination therapy and gene therapy are new strategies to potentially overcome the limitations of TRAIL, however, the lack of efficient and low toxic vectors remains the major obstacle. In this study, we developed a hyaluronic acid (HA)-decorated polyethylenimine-poly(d,l-lactide-co-glycolide) (PEI-PLGA) nanoparticle (NP) system for targeted co-delivery of TRAIL plasmid (pTRAIL) and gambogic acid (GA) in triple-negative breast cancer (TNBC) therapy. GA was encapsulated into the core of the PEI-PLGA NPs while pTRAIL was adsorbed onto the positive NP surface via charge adsorption. The coating of HA on PEI-PLGA NPs functions as a targeting ligand by binding to CD44 receptor of TNBC cells and a shell to neutralize the excess positive charge of inner NPs. The resultant pTRAIL and GA co-loaded HA-coated PEI-PLGA NPs exhibited spherical shape (121.5 nm) and could promote the internalization of loaded cargoes into TNBC cells through the CD44-dependent endocytic pathway. The dual drug-loaded NPs significantly augmented apoptotic cell death in vitro and inhibited TNBC tumor growth in vivo. This multifunctional NP system efficiently co-delivered GA and pTRAIL, thus representing a promising strategy to treat TNBC and bringing forth a platform strategy for co-delivery of therapeutic DNA and chemotherapeutic agents in combinatorial TNBC therapy.
[Mh] Termos MeSH primário: Ácido Hialurônico/administração & dosagem
Ácido Láctico/administração & dosagem
Nanopartículas/administração & dosagem
Plasmídeos/administração & dosagem
Polietilenoimina/administração & dosagem
Ácido Poliglicólico/administração & dosagem
Ligante Indutor de Apoptose Relacionado a TNF/administração & dosagem
Neoplasias de Mama Triplo Negativas/tratamento farmacológico
Xantonas/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/administração & dosagem
Linhagem Celular Tumoral
Portadores de Fármacos/administração & dosagem
Seres Humanos
Receptores de Hialuronatos/metabolismo
Células MCF-7
Camundongos
Neoplasias de Mama Triplo Negativas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Drug Carriers); 0 (Hyaluronan Receptors); 0 (TNF-Related Apoptosis-Inducing Ligand); 0 (Xanthones); 0 (polylactic acid-polyglycolic acid copolymer); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); 8N585K83U2 (gambogic acid); 9002-98-6 (Polyethyleneimine); 9004-61-9 (Hyaluronic Acid)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180130
[Lr] Data última revisão:
180130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1080/10717544.2017.1406558


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[PMID]:27770422
[Au] Autor:Hickey DP
[Ad] Endereço:Department of Chemistry, University of Utah, 315 S 1400 E, Salt Lake City, UT, 84112, USA. dhickeychem@gmail.com.
[Ti] Título:Ferrocene-Modified Linear Poly(ethylenimine) for Enzymatic Immobilization and Electron Mediation.
[So] Source:Methods Mol Biol;1504:181-191, 2017.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enzymatic glucose biosensors and biofuel cells make use of the electrochemical transduction between an oxidoreductase enzyme, such as glucose oxidase (GOx), and an electrode to either quantify the amount of glucose in a solution or generate electrical energy. However, many enzymes including GOx are not able to electrochemically interact with an electrode surface directly, but require an external electrochemical relay to shuttle electrons to the electrode. Ferrocene-modified linear poly(ethylenimine) (Fc-LPEI) redox polymers have been designed to simultaneously immobilize glucose oxidase (GOx) at an electrode and mediate electron transfer from their flavin adenine dinucleotide (FAD) active site to the electrode surface. Cross-linked films of Fc-LPEI create hydrogel networks that allow for rapid transport of glucose, while the covalently bound ferrocene moieties are able to facilitate rapid electron transfer due to the ability of ferrocene to exchange electrons between adjacent ferrocene residues. For these reasons, Fc-LPEI films have been widely used in the development of high current density bioanode materials. This chapter describes the synthesis of a commonly used dimethylferrocene-modified linear poly(ethylenimine), as well as the subsequent preparation and electrochemical characterization of a GOx bioanode film utilizing the synthesized polymer.
[Mh] Termos MeSH primário: Aspergillus niger/enzimologia
Técnicas Biossensoriais/métodos
Enzimas Imobilizadas/química
Compostos Ferrosos/química
Glucose Oxidase/química
Glucose/análise
Metalocenos/química
Polietilenoimina/análogos & derivados
[Mh] Termos MeSH secundário: Aspergillus niger/química
Aspergillus niger/metabolismo
Eletrodos
Transporte de Elétrons
Elétrons
Enzimas Imobilizadas/metabolismo
Glucose/metabolismo
Glucose Oxidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzymes, Immobilized); 0 (Ferrous Compounds); 0 (Metallocenes); 9002-98-6 (Polyethyleneimine); EC 1.1.3.4 (Glucose Oxidase); IY9XDZ35W2 (Glucose); U96PKG90JQ (ferrocene)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE


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[PMID]:28456652
[Au] Autor:Schwabe K; Ewe A; Kohn C; Loth T; Aigner A; Hacker MC; Schulz-Siegmund M
[Ad] Endereço:Leipzig University, Institute of Pharmacy, Pharmaceutical Technology, Germany.
[Ti] Título:Sustained delivery of siRNA poly- and lipopolyplexes from porous macromer-crosslinked gelatin gels.
[So] Source:Int J Pharm;526(1-2):178-187, 2017 Jun 30.
[Is] ISSN:1873-3476
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:RNA interference (RNAi) is a promising technique to treat severe diseases on a pre-protein level. We and others postulate that the release of nanoparticle-complexed small interfering RNA (siRNA) from implanted biomaterials could provide structural support for tissue repair, combined with local siRNA transfection of invading and regenerating cells. In this study, we systematically investigated cross-linked gelatin based hydrogel formulations (cGEL) as degradable controlled release matrices for siRNA. Aiming at the definition of correlations between cGEL composition, siRNA nanoparticle formulation, release kinetics of complexed siRNA and transfection efficiency, we combined five different cGEL formulations and three transfection systems, i.e. polyplexes with polyethyleneimine (PEI), PEI in combination with liposomes (lipopolyplexes) and polyplexes based on tyrosin-modified PEI (P10Y). It was found that the distribution of these poly-/lipopolyplexes, when applied onto the negatively charged hydrogels, was strongly dependent on their zeta potential. Furthermore, siRNA release from the hydrogel was a multifactorial process, as diffusion, hydrogel degradation and nanoparticle decomplexation overlapped over time. This resulted in a prolonged release of siRNA for up to 21days. In the case of PEI complexes and lipopolyplexes, release kinetics depended on the cGEL formulation. In contrast, when employing P10Y polyplexes, an initial burst release was observed with no further release thereafter. Silencing activity was determined using constitutively luciferase-expressing SKOV-3-Luc reporter cells. Surface and bulk porosity in hydrogels was introduced by addition of soluble polyethylene glycol during fabrication, leading to improved knockdown. The rapid onset of knockdown efficacy will also provide the basis for the determination of long-term effects.
[Mh] Termos MeSH primário: Gelatina/química
Hidrogéis/química
RNA Interferente Pequeno/administração & dosagem
Transfecção/métodos
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Seres Humanos
Polietilenoimina
Interferência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrogels); 0 (RNA, Small Interfering); 9000-70-8 (Gelatin); 9002-98-6 (Polyethyleneimine)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


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[PMID]:28873642
[Au] Autor:Zhou M; Khen K; Wang T; Hu Q; Xue J; Luo Y
[Ad] Endereço:Department of Nutritional Sciences, University of Connecticut, Storrs, CT 06269, USA.
[Ti] Título:Chemical crosslinking improves the gastrointestinal stability and enhances nutrient delivery potentials of egg yolk LDL/polysaccharide nanogels.
[So] Source:Food Chem;239:840-847, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Egg yolk low density lipoprotein (LDL)/polysaccharide nanogels are newly explored as oral delivery systems with promising encapsulation potentials. Nonetheless, the stability of nanogels against aggregation in gastrointestinal tract remains a challenge. Therefore, chemical crosslinking by 1-ethyl-3-(3-dimethylaminopropyl) and carbodiimide/N-hydroxysuccinimide (EDC/NHS) was adopted to improve the gastrointestinal stability of nanogels. Compared to original uncrosslinked nanogels, crosslinking did not change particle size, polydispersity index (PDI) and morphology, but it reduced surface charge of nanogels. The nano spray dried LDL/CMC/EDC nanogels had relatively poor surface structure with agglomerations. The FT-IR spectra confirmed the formation of new peptide bonds, which significantly improved stability of nanogels under simulated gastrointestinal conditions. Fluorescence spectra evidenced that non-polar microenvironment for curcumin embedded in nanogels was strengthened, which therefore enhanced encapsulation efficiency. Moreover, curcumin exhibited sustained release profile from crosslinked nanogels in simulated gastrointestinal fluids. Overall, our study provided a promising strategy to enhance the stability of LDL-based nanogels in digestive conditions.
[Mh] Termos MeSH primário: Gema de Ovo
[Mh] Termos MeSH secundário: Polietilenoglicóis
Polietilenoimina
Polissacarídeos
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NanoGel); 0 (Polysaccharides); 30IQX730WE (Polyethylene Glycols); 9002-98-6 (Polyethyleneimine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


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[PMID]:29041937
[Au] Autor:Hamdous Y; Chebbi I; Mandawala C; Le Fèvre R; Guyot F; Seksek O; Alphandéry E
[Ad] Endereço:Nanobacterie, 36 boulevard Flandrin, 75116, Paris, France.
[Ti] Título:Biocompatible coated magnetosome minerals with various organization and cellular interaction properties induce cytotoxicity towards RG-2 and GL-261 glioma cells in the presence of an alternating magnetic field.
[So] Source:J Nanobiotechnology;15(1):74, 2017 Oct 17.
[Is] ISSN:1477-3155
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Biologics magnetics nanoparticles, magnetosomes, attract attention because of their magnetic characteristics and potential applications. The aim of the present study was to develop and characterize novel magnetosomes, which were extracted from magnetotactic bacteria, purified to produce apyrogen magnetosome minerals, and then coated with Chitosan, Neridronate, or Polyethyleneimine. It yielded stable magnetosomes designated as M-Chi, M-Neri, and M-PEI, respectively. Nanoparticle biocompatibility was evaluated on mouse fibroblast cells (3T3), mouse glioblastoma cells (GL-261) and rat glioblastoma cells (RG-2). We also tested these nanoparticles for magnetic hyperthermia treatment of tumor in vitro on two tumor cell lines GL-261 and RG-2 under the application of an alternating magnetic field. Heating, efficacy and internalization properties were then evaluated. RESULTS: Nanoparticles coated with chitosan, polyethyleneimine and neridronate are apyrogen, biocompatible and stable in aqueous suspension. The presence of a thin coating in M-Chi and M-PEI favors an arrangement in chains of the magnetosomes, similar to that observed in magnetosomes directly extracted from magnetotactic bacteria, while the thick matrix embedding M-Neri leads to structures with an average thickness of 3.5 µm per magnetosome mineral. In the presence of GL-261 cells and upon the application of an alternating magnetic field, M-PEI and M-Chi lead to the highest specific absorption rates of 120-125 W/g . Furthermore, while M-Chi lead to rather low rates of cellular internalization, M-PEI strongly associate to cells, a property modulated by the application of an alternating magnetic field. CONCLUSIONS: Coating of purified magnetosome minerals can therefore be chosen to control the interactions of nanoparticles with cells, organization of the minerals, as well as heating and cytotoxicity properties, which are important parameters to be considered in the design of a magnetic hyperthermia treatment of tumor.
[Mh] Termos MeSH primário: Materiais Revestidos Biocompatíveis/química
Materiais Revestidos Biocompatíveis/uso terapêutico
Glioma/terapia
Magnetossomos/química
Nanopartículas/química
Nanopartículas/uso terapêutico
[Mh] Termos MeSH secundário: Células 3T3
Animais
Linhagem Celular Tumoral
Quitosana/química
Quitosana/uso terapêutico
Difosfonatos/química
Difosfonatos/uso terapêutico
Hipertermia Induzida
Campos Magnéticos
Magnetospirillum/química
Camundongos
Polietilenoimina/química
Polietilenoimina/uso terapêutico
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coated Materials, Biocompatible); 0 (Diphosphonates); 79778-41-9 (6-amino-1-hydroxyhexane-1,1-diphosphonate); 9002-98-6 (Polyethyleneimine); 9012-76-4 (Chitosan)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171019
[St] Status:MEDLINE
[do] DOI:10.1186/s12951-017-0293-2


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[PMID]:28910855
[Au] Autor:Yang S; Zhou X; Li R; Fu X; Sun P
[Ad] Endereço:Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou, People's Republic of China.
[Ti] Título:Optimized PEI-based Transfection Method for Transient Transfection and Lentiviral Production.
[So] Source:Curr Protoc Chem Biol;9(3):147-157, 2017 Sep 14.
[Is] ISSN:2160-4762
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Polyethyleneimine (PEI), a cationic polymer vehicle, forms a complex with DNA which then can carry anionic nucleic acids into eukaryotic cells. PEI-based transfection is widely used for transient transfection of plasmid DNA. The efficiency of PEI-based transfection is affected by numerous factors, including the way the PEI/DNA complex is prepared, the ratio of PEI to DNA, the concentration of DNA, the storage conditions of PEI solutions, and more. Considering the major influencing factors, PEI-based transfection has been optimized to improve its efficiency, reproducibility, and consistency. This protocol outlines the steps for ordinary transient transfection and lentiviral production using PEI. © 2017 by John Wiley & Sons, Inc.
[Mh] Termos MeSH primário: Lentivirus/crescimento & desenvolvimento
Lentivirus/genética
Polietilenoimina/química
Transfecção/métodos
[Mh] Termos MeSH secundário: DNA/genética
DNA/metabolismo
Seres Humanos
Plasmídeos/genética
Plasmídeos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9002-98-6 (Polyethyleneimine); 9007-49-2 (DNA)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1002/cpch.25


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[PMID]:28841789
[Au] Autor:Jin X; Xiang Z; Liu Q; Chen Y; Lu F
[Ad] Endereço:State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China.
[Ti] Título:Polyethyleneimine-bacterial cellulose bioadsorbent for effective removal of copper and lead ions from aqueous solution.
[So] Source:Bioresour Technol;244(Pt 1):844-849, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bacterial cellulose (BC) is a green biopolymer suitable for heavy metal ion removal from aqueous solution due to its nano-porous microstructure. Polyethyleneimine-bacterial cellulose (PEI-BC) was prepared by reductive amination of dialdehyde BC with polyethyleneimine. The capacity of PEI-BC in Cu(II) and Pb(II) adsorption from aqueous solution was investigated. The adsorption kinetics could be well expressed by pseudo-second-order model and the adsorption isotherm data were well fitted with Freundlich model. Adsorption processes of Cu(II) and Pb(II) by PEI-BC reached equilibrium very rapid in 30 and 60min, respectively. The maximum adsorption capacity of PEI-BC on Cu(II) and Pb(II) was found to be 148 and 141mg/g, respectively, which was higher than that of unmodified BC and other modified BC reported. PEI-BC also showed good reusability in the adsorption of Cu(II) and Pb(II). This study demonstrates that polyethyleneimine modification makes BC a potential bioadsorbent for heavy metal ion removal in waste water.
[Mh] Termos MeSH primário: Celulose
Polietilenoimina
Purificação da Água
[Mh] Termos MeSH secundário: Adsorção
Cobre
Íons
Cinética
Chumbo
Poluentes Químicos da Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ions); 0 (Water Pollutants, Chemical); 2P299V784P (Lead); 789U1901C5 (Copper); 9002-98-6 (Polyethyleneimine); 9004-34-6 (Cellulose)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170827
[St] Status:MEDLINE



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