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[PMID]:29231221
[Au] Autor:Cozzoli L; Gjonaj L; Stuart MCA; Poolman B; Roelfes G
[Ad] Endereço:Stratingh Institute for Chemistry, Nijenborgh 4, 9747 AG Groningen, The Netherlands. j.g.roelfes@rug.nl.
[Ti] Título:Responsive DNA G-quadruplex micelles.
[So] Source:Chem Commun (Camb);54(3):260-263, 2018 Jan 02.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel and versatile design of DNA-lipid conjugates is presented. The assembly of the DNA headgroups into G-quadruplex structures is essential for the formation of micelles and their stability. By hybridization with a complementary oligonucleotide the micelles were destabilized, resulting in cargo release. In combination with a hairpin DNA aptamer as complementary strand, the release is obtained selectively by the presence of ATP.
[Mh] Termos MeSH primário: DNA/química
Portadores de Fármacos/química
Quadruplex G
Lipídeos/química
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/química
Animais
Aptâmeros de Nucleotídeos/genética
Carbocianinas/química
Bovinos
DNA/genética
Liberação Controlada de Fármacos
Corantes Fluorescentes/química
Micelas
Hibridização de Ácido Nucleico
Oxazinas/química
Soroalbumina Bovina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aptamers, Nucleotide); 0 (Carbocyanines); 0 (Drug Carriers); 0 (Fluorescent Dyes); 0 (Lipids); 0 (Micelles); 0 (Oxazines); 27432CM55Q (Serum Albumin, Bovine); 40957-95-7 (3,3'-dioctadecylindocarbocyanine); 68006-80-4 (3,3'-dioctadecyloxacarbocyanine); 8L70Q75FXE (Adenosine Triphosphate); 9007-49-2 (DNA); P476F1L81G (nile red)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1039/c7cc07899d


  2 / 4396 MEDLINE  
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[PMID]:29211987
[Au] Autor:Liu Y; Lilley DMJ
[Ad] Endereço:Cancer Research UK Nucleic Acid Structure Research Group, MSI/WTB Complex, The University of Dundee, Dundee, United Kingdom.
[Ti] Título:Crystal Structures of Cyanine Fluorophores Stacked onto the End of Double-Stranded RNA.
[So] Source:Biophys J;113(11):2336-2343, 2017 Dec 05.
[Is] ISSN:1542-0086
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The indodicarbocyanine fluorophores Cy3 and Cy5 are extensively used as donor-acceptor pairs in fluorescence resonance energy transfer experiments, especially those involving single molecules. When terminally attached to double-stranded nucleic acids via the 5' phosphate group these fluorophores stack onto the ends of the molecule. Knowledge of the positions of the fluorophores is critical to the interpretation of fluorescence resonance energy transfer data. The positions have been demonstrated for double-stranded (ds) DNA using NMR spectroscopy. Here, we have used x-ray crystallography to analyze the location of Cy3 and Cy5 on dsRNA, using complexes of an RNA stem-loop bound to L5 protein determined at 2.4 Å resolution. This confirms the tendency of both fluorophores to stack on the free end of RNA, with the long axis of the fluorophores approximately parallel to that of the terminal basepair. However, the manner of interaction of both Cy3 and Cy5 with the terminus of the dsRNA is significantly different from that deduced for dsDNA using NMR. The fluorophores are stacked on the terminal basepair such that their indole nitrogen atoms lie on the major groove side, and thus their pendant methyl groups are on the minor groove side.
[Mh] Termos MeSH primário: Carbocianinas/química
Corantes Fluorescentes/química
RNA de Cadeia Dupla/química
[Mh] Termos MeSH secundário: Proteínas de Bactérias/química
Proteínas de Bactérias/metabolismo
Pareamento de Bases
Cristalografia por Raios X
Modelos Moleculares
Conformação Proteica
RNA de Cadeia Dupla/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Carbocyanines); 0 (Fluorescent Dyes); 0 (RNA, Double-Stranded)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180120
[Lr] Data última revisão:
180120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


  3 / 4396 MEDLINE  
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Suzuki, Akemi
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[PMID]:27773703
[Au] Autor:Hirano M; Totani K; Fukuda T; Gu J; Suzuki A
[Ad] Endereço:Institute of Glycoscience, Tokai University, 4-1-1 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan; Department of Materials and Life Science, Faculty of Science and Technology, Seikei University, 3-3-1 Kichijoji-kita, Musashino, Tokyo 180-8633, Japan. Electronic address: mhirano@st.seikei.ac.jp.
[Ti] Título:N-Glycoform-dependent interactions of megalin with its ligands.
[So] Source:Biochim Biophys Acta;1861(1 Pt A):3106-3118, 2017 01.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Megalin is a 600-kDa single-spanning transmembrane glycoprotein and functions as an endocytic receptor, distributed not only in the kidney but also in other tissues. Structurally and functionally distinct ligands for megalin have been identified. Megalin has 30 potential N-glycosylation sites in its extracellular domain. We found that megalin interacts with its ligands in a glycoform-dependent manner. METHODS: Distribution of megalin and glycans was histochemically analyzed in mouse kidneys. Kidney absorption of Cy5-labeled ligands was examined in vivo. Megalin-ligand interactions were analyzed using ligand blotting and ELISA. RESULTS: Megalins expressed on renal proximal convoluted tubules (PCTs) and proximal straight tubules (PSTs) have different N-glycans. PCT megalin stained with Lens culinaris agglutinin (LCA), which recognizes core-fucosyl N-glycans catalyzed by α1,6-fucosyltransferase (Fut8). In contrast, PST megalin stained with wheat germ agglutinin (WGA), which recognizes hybrid-type N-glycans. Retinol-binding protein-Cy5 (RBP-Cy5) was endocytosed by megalin on PCTs but minimally endocytosed by PSTs. BSA-Cy5 was endocytosed nearly equally by both tubules. The purified LCA-positive glycoform megalin had higher binding activity for RBP and vitamin D-binding protein than did WGA-positive glycoform megalin. Both glycoforms had nearly the same BSA- and kanamycin-binding activities. RBP-binding analysis of megalin lacking core fucose, in Fut8 mouse kidneys, had significantly decreased binding activity. CONCLUSIONS: N-Glycosylation of megalin can modulate its ligand-binding activity. Core fucosylation, in particular, is a modification crucial for megalin-RBP interactions. GENERAL SIGNIFICANCE: Cell type-specific glycoforms of megalin exist in the proximal tubular cells and modulate ligand absorption capacity.
[Mh] Termos MeSH primário: Proteína-2 Relacionada a Receptor de Lipoproteína de Baixa Densidade/metabolismo
Polissacarídeos/metabolismo
[Mh] Termos MeSH secundário: Animais
Carbocianinas/metabolismo
Cromatografia de Afinidade
Feminino
Fucosiltransferases/deficiência
Fucosiltransferases/metabolismo
Glicosilação
Rim/metabolismo
Túbulos Renais Proximais/citologia
Túbulos Renais Proximais/metabolismo
Ligantes
Camundongos Endogâmicos C57BL
Camundongos Knockout
Especificidade de Órgãos
Lectinas de Plantas/metabolismo
Ligação Proteica
Proteínas de Ligação ao Retinol/metabolismo
Aglutininas do Germe de Trigo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carbocyanines); 0 (Ligands); 0 (Low Density Lipoprotein Receptor-Related Protein-2); 0 (Plant Lectins); 0 (Polysaccharides); 0 (Retinol-Binding Proteins); 0 (Wheat Germ Agglutinins); 0 (cyanine dye 5); 0 (lentil lectin); EC 2.4.1.- (Fucosyltransferases); EC 2.4.1.68 (Glycoprotein 6-alpha-L-fucosyltransferase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171224
[Lr] Data última revisão:
171224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


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[PMID]:28965786
[Au] Autor:Miranda P; Oliveira LM; Weber G
[Ad] Endereço:Departamento de Física, Universidade Federal de Minas Gerais, Belo Horizonte-MG 31270-901, Brazil.
[Ti] Título:Mesoscopic modelling of Cy3 and Cy5 dyes attached to DNA duplexes.
[So] Source:Biophys Chem;230:62-67, 2017 Nov.
[Is] ISSN:1873-4200
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cy3 and Cy5 dyes linked to the 5' end of a double stranded DNA molecule are known to attach to both strands in a way that is very similar to an additional base pair and has a stabilizing effect on the oligonucleotide. Here we adapt the Peyrard-Bishop mesoscopic model to incorporate cyanine dyes and use the technique of thermal equivalence to obtain the appropriate parameters from existing melting temperatures. We have found that the stacking parameters are in the same range of ordinary AT and CG base pairs, in particular Cy3-A was found to be most rigidly stacked. While the cyanines stabilize the AT hydrogen bonds quite strongly the CG bonds are mostly unaffected.
[Mh] Termos MeSH primário: Carbocianinas/química
DNA/química
[Mh] Termos MeSH secundário: Pareamento de Bases
Carbocianinas/metabolismo
DNA/metabolismo
Ligações de Hidrogênio
Modelos Moleculares
Desnaturação de Ácido Nucleico
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbocyanines); 0 (cyanine dye 3); 0 (cyanine dye 5); 9007-49-2 (DNA)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171003
[St] Status:MEDLINE


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[PMID]:28817123
[Au] Autor:Sharifi-Sanjani M; Meeker AK; Mourkioti F
[Ad] Endereço:Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
[Ti] Título:Evaluation of telomere length in human cardiac tissues using cardiac quantitative FISH.
[So] Source:Nat Protoc;12(9):1855-1870, 2017 Sep.
[Is] ISSN:1750-2799
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Telomere length has been correlated with various diseases, including cardiovascular disease and cancer. The use of currently available telomere-length measurement techniques is often restricted by the requirement of a large amount of cells (Southern-based techniques) or the lack of information on individual cells or telomeres (PCR-based methods). Although several methods have been used to measure telomere length in tissues as a whole, the assessment of cell-type-specific telomere length provides valuable information on individual cell types. The development of fluorescence in situ hybridization (FISH) technologies enables the quantification of telomeres in individual chromosomes, but the use of these methods is dependent on the availability of isolated cells, which prevents their use with fixed archival samples. Here we describe an optimized quantitative FISH (Q-FISH) protocol for measuring telomere length that bypasses the previous limitations by avoiding contributions from undesired cell types. We have used this protocol on small paraffin-embedded cardiac-tissue samples. This protocol describes step-by-step procedures for tissue preparation, permeabilization, cardiac-tissue pretreatment and hybridization with a Cy3-labeled telomeric repeat complementing (CCCTAA) peptide nucleic acid (PNA) probe coupled with cardiac-specific antibody staining. We also describe how to quantify telomere length by means of the fluorescence intensity and area of each telomere within individual nuclei. This protocol provides comparative cell-type-specific telomere-length measurements in relatively small human cardiac samples and offers an attractive technique to test hypotheses implicating telomere length in various cardiac pathologies. The current protocol (from tissue collection to image procurement) takes ∼28 h along with three overnight incubations. We anticipate that the protocol could be easily adapted for use on different tissue types.
[Mh] Termos MeSH primário: Hibridização in Situ Fluorescente/métodos
Miocárdio/química
Telômero/química
[Mh] Termos MeSH secundário: Carbocianinas/química
Seres Humanos
Ácidos Nucleicos Peptídicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbocyanines); 0 (Peptide Nucleic Acids); 0 (cyanine dye 3)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1038/nprot.2017.082


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[PMID]:28781168
[Au] Autor:Wong HH; Lin JQ; Ströhl F; Roque CG; Cioni JM; Cagnetta R; Turner-Bridger B; Laine RF; Harris WA; Kaminski CF; Holt CE
[Ad] Endereço:Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UK.
[Ti] Título:RNA Docking and Local Translation Regulate Site-Specific Axon Remodeling In Vivo.
[So] Source:Neuron;95(4):852-868.e8, 2017 Aug 16.
[Is] ISSN:1097-4199
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nascent proteins can be positioned rapidly at precise subcellular locations by local protein synthesis (LPS) to facilitate localized growth responses. Axon arbor architecture, a major determinant of synaptic connectivity, is shaped by localized growth responses, but it is unknown whether LPS influences these responses in vivo. Using high-resolution live imaging, we examined the spatiotemporal dynamics of RNA and LPS in retinal axons during arborization in vivo. Endogenous RNA tracking reveals that RNA granules dock at sites of branch emergence and invade stabilized branches. Live translation reporter analysis reveals that de novo ß-actin hotspots colocalize with docked RNA granules at the bases and tips of new branches. Inhibition of axonal ß-actin mRNA translation disrupts arbor dynamics primarily by reducing new branch emergence and leads to impoverished terminal arbors. The results demonstrate a requirement for LPS in building arbor complexity and suggest a key role for pre-synaptic LPS in assembling neural circuits.
[Mh] Termos MeSH primário: Axônios/fisiologia
Regulação da Expressão Gênica no Desenvolvimento/genética
RNA/metabolismo
[Mh] Termos MeSH secundário: Actinas/genética
Actinas/metabolismo
Animais
Anisomicina/farmacologia
Biotina/metabolismo
Blastômeros
Carbocianinas/metabolismo
Cicloeximida/farmacologia
Nucleotídeos de Desoxiuracil/metabolismo
Embrião não Mamífero
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Técnicas In Vitro
Proteínas Luminescentes/genética
Proteínas Luminescentes/metabolismo
Mitocôndrias/metabolismo
Morfolinos/farmacologia
Oligonucleotídeos Antissenso/farmacologia
Técnicas de Cultura de Órgãos
Inibidores da Síntese de Proteínas/farmacologia
RNA/genética
Retina/citologia
Xenopus laevis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3'-deoxy-5-(cyanine dye 5)uridine 5'-trisphosphate); 0 (Actins); 0 (Carbocyanines); 0 (Deoxyuracil Nucleotides); 0 (Luminescent Proteins); 0 (Morpholinos); 0 (Oligonucleotides, Antisense); 0 (Protein Synthesis Inhibitors); 63231-63-0 (RNA); 6C74YM2NGI (Anisomycin); 6SO6U10H04 (Biotin); 98600C0908 (Cycloheximide)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170902
[Lr] Data última revisão:
170902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE


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[PMID]:28780040
[Au] Autor:Schneider JR; Carias AM; Bastian AR; Cianci GC; Kiser PF; Veazey RS; Hope TJ
[Ad] Endereço:Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
[Ti] Título:Long-term direct visualization of passively transferred fluorophore-conjugated antibodies.
[So] Source:J Immunol Methods;450:66-72, 2017 Nov.
[Is] ISSN:1872-7905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The use of therapeutic antibodies, delivered by intravenous (IV) instillation, is a rapidly expanding area of biomedical treatment for a variety of conditions. However, little is known about how the antibodies are anatomically distributed following infusion and the underlying mechanism mediating therapeutic antibody distribution to specific anatomical sites remains to be elucidated. Current efforts utilize low resolution and sensitivity methods such as ELISA and indirect labeling imaging techniques, which often leads to high background and difficulty in assessing biodistribution. Here, using the in vivo non-human primate model, we demonstrate that it is possible to utilize the fluorophores Cy5 and Cy3 directly conjugated to antibodies for direct visualization and quantification of passively transferred antibodies in plasma, tissue, and in mucosal secretions. Antibodies were formulated with 1-2 fluorophores per antibody to minimally influence antibody function. Fluorophore conjugated Gamunex-C (pooled human IgG) were tested for binding to protein A, via surface plasmon resonance, and showed similar levels of binding when compared to unlabeled Gamunex-C. In order to assess the effect fluorophore labeling has on turnover and localization, rhesus macaques were IV infused with either labeled or unlabeled Gamunex-C. Plasma, vaginal Weck-Cel fluid, cervicovaginal mucus, and vaginal/rectal tissue biopsies were collected up to 8weeks. Similar turnover and biodistribution was observed between labeled and unlabeled antibodies, showing that the labeling process did not have an obvious deleterious effect on localization or turnover. Cy5 and Cy3 labeled antibodies were readily detected in the same pattern regardless of fluorophore. Tissue distribution was measured in macaque vaginal and rectal biopsies. The labeled antibody in macaque biopsies was found to have similar biodistribution pattern to endogenous antibodies in macaque and human tissues. In the vaginal and rectal mucosa, endogenous and infused antibodies were found primarily within the lamina propria. In the mucosal squamous epithelium of the vaginal vault, significant antibody was also observed in a striated pattern in the superficial, nonviable, stratum corneum. Endogenous antibody distribution in both human and macaque squamous tissues exhibited a similar pattern as seen with the labeled and unlabeled antibodies. This proof-of-principle study reveals that the labeled antibody is stable and physiologically similar relative to endogenous antibody setting the stage for future work to better understand the mechanisms of how antibodies reach unique anatomical sites. Direct visualization of fluorophore-conjugated antibodies following passive infusion can be utilized to assess the kinetics of biodistribution of infused antibodies and may be a useful approach to monitor and predict efficacy of therapeutic antibodies.
[Mh] Termos MeSH primário: Carbocianinas/metabolismo
Imunofluorescência
Corantes Fluorescentes/metabolismo
Imunoglobulinas Intravenosas/sangue
Microscopia de Fluorescência
[Mh] Termos MeSH secundário: Animais
Carbocianinas/administração & dosagem
Carbocianinas/química
Muco do Colo Uterino/metabolismo
Muco do Colo Uterino/secreção
Estabilidade de Medicamentos
Feminino
Corantes Fluorescentes/administração & dosagem
Corantes Fluorescentes/química
Seres Humanos
Imunoglobulinas Intravenosas/administração & dosagem
Imunoglobulinas Intravenosas/química
Imunoglobulinas Intravenosas/farmacocinética
Infusões Intravenosas
Macaca mulatta
Modelos Animais
Membrana Mucosa/metabolismo
Membrana Mucosa/secreção
Plasma/metabolismo
Estabilidade Proteica
Reto/metabolismo
Ressonância de Plasmônio de Superfície
Distribuição Tecidual
Vagina/metabolismo
Vagina/secreção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbocyanines); 0 (Fluorescent Dyes); 0 (Immunoglobulins, Intravenous); 0 (cyanine dye 3); 0 (cyanine dye 5)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170807
[St] Status:MEDLINE


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[PMID]:28704573
[Au] Autor:Negwer I; Hirsch M; Kaloyanova S; Brown T; Peneva K; Butt HJ; Koynov K; Helm M
[Ad] Endereço:Pharmaceutical Chemistry, Institute of Pharmacy and Biochemistry, Johannes Gutenberg University, Staudinger Weg 5, 55128, Mainz, Germany.
[Ti] Título:Modulation of Mitochondriotropic Properties of Cyanine Dyes by in Organello Copper-Free Click Reaction.
[So] Source:Chembiochem;18(18):1814-1818, 2017 Sep 19.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Cyanine (Cy) dyes show a general propensity to localize in polarized mitochondria. This mitochondriotropism was used to perform a copper-free click reaction in the mitochondria of living cells. The in organello reaction of dyes Cy3 and Cy5 led to a product that was easily traceable by Förster resonance energy transfer (FRET). As determined by confocal laser scanning microscopy, the Cy3-Cy5 conjugate showed enhanced retention in mitochondria, relative to that of the starting compounds. This enhancement of a favorable property can be achieved by synthesis in organello, but not outside mitochondria.
[Mh] Termos MeSH primário: Carbocianinas/metabolismo
Corantes Fluorescentes/metabolismo
Mitocôndrias/metabolismo
[Mh] Termos MeSH secundário: Animais
Carbocianinas/química
Linhagem Celular
Química Click
Cobre/química
Transferência Ressonante de Energia de Fluorescência
Corantes Fluorescentes/química
Microscopia Confocal
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbocyanines); 0 (Fluorescent Dyes); 0 (cyanine dye 3); 789U1901C5 (Copper)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201700286


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[PMID]:28682052
[Au] Autor:Wang W; Zhu Y; Chen X
[Ad] Endereço:College of Chemistry and Molecular Engineering, ‡Peking-Tsinghua Center for Life Sciences, §Synthetic and Functional Biomolecules Center, and ∥Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Peking University , Beijing 100871, China.
[Ti] Título:Selective Imaging of Gram-Negative and Gram-Positive Microbiotas in the Mouse Gut.
[So] Source:Biochemistry;56(30):3889-3893, 2017 Aug 01.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The diverse gut microbial communities are crucial for host health. How the interactions between microbial communities and between host and microbes influence the host, however, is not well understood. To facilitate gut microbiota research, selective imaging of specific groups of microbiotas in the gut is of great utility but remains technically challenging. Here we present a chemical approach that enables selective imaging of Gram-negative and Gram-positive microbiotas in the mouse gut by exploiting their distinctive cell wall components. Cell-selective labeling is achieved by the combined use of metabolic labeling of Gram-negative bacterial lipopolysaccharides with a clickable azidosugar and direct labeling of Gram-positive bacteria with a vancomycin-derivatized fluorescent probe. We demonstrated this strategy by two-color fluorescence imaging of Gram-negative and Gram-positive gut microbiotas in the mouse intestines. This chemical method should be broadly applicable to different gut microbiota research fields and other bacterial communities studied in microbiology.
[Mh] Termos MeSH primário: Técnicas de Diagnóstico do Sistema Digestório
Disbiose/diagnóstico por imagem
Microbioma Gastrointestinal
Trato Gastrointestinal/diagnóstico por imagem
Bactérias Gram-Negativas/isolamento & purificação
Bactérias Gram-Positivas/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Azidas/análise
Azidas/química
Azidas/metabolismo
Azidas/farmacologia
Carbocianinas/análise
Parede Celular/química
Química Click
Disbiose/microbiologia
Corantes Fluorescentes/análise
Corantes Fluorescentes/química
Corantes Fluorescentes/metabolismo
Corantes Fluorescentes/farmacologia
Trato Gastrointestinal/microbiologia
Bactérias Gram-Negativas/citologia
Bactérias Gram-Negativas/crescimento & desenvolvimento
Bactérias Gram-Negativas/metabolismo
Bactérias Gram-Positivas/citologia
Bactérias Gram-Positivas/crescimento & desenvolvimento
Bactérias Gram-Positivas/metabolismo
Lipopolissacarídeos/análise
Lipopolissacarídeos/biossíntese
Lipopolissacarídeos/química
Camundongos Endogâmicos C57BL
Viabilidade Microbiana/efeitos dos fármacos
Imagem Óptica
Projetos Piloto
Porfobilinogênio/análogos & derivados
Porfobilinogênio/análise
Porfobilinogênio/química
Rodaminas/análise
Rodaminas/química
Organismos Livres de Patógenos Específicos
Açúcares Ácidos/análise
Açúcares Ácidos/química
Açúcares Ácidos/metabolismo
Açúcares Ácidos/farmacologia
Vancomicina/análogos & derivados
Vancomicina/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (3-anilino difluoroboron dipyrromethene); 0 (5-carboxytetramethylrhodamine succinimidyl ester); 0 (8-azido-3,8-dideoxyoctulosonate); 0 (Alexa Fluor 647); 0 (Azides); 0 (Carbocyanines); 0 (Fluorescent Dyes); 0 (Lipopolysaccharides); 0 (Rhodamines); 0 (Sugar Acids); 1069-03-0 (2-keto-3-deoxyoctonate); 6Q205EH1VU (Vancomycin); 74KHC72QXK (Porphobilinogen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00539


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Fotocópia
[PMID]:28668840
[Au] Autor:Schmeel LC; Schmeel FC; Schmidt-Wolf IGH
[Ad] Endereço:Department of Radiology and Radiation Oncology, University Hospital Bonn, Bonn, Germany.
[Ti] Título: Apoptosis Induction by Fenofibrate in Lymphoma and Multiple Myeloma.
[So] Source:Anticancer Res;37(7):3513-3520, 2017 07.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIM: Recent innovations in the treatment of multiple myeloma have enriched our therapeutic repertoire regarding the treatment of multiple myeloma during the last decades. However, despite today's therapies many multiple myeloma (MM) patients experience relapse of disease and eventually remain incurable. Wnt/ß-catenin signaling has been demonstrated in lymphoma and MM, rendering related signaling molecules promising therapeutic targets. Fenofibrate, an extensively scrutinized and widely used drug for primary hypercholesterolemia or mixed dyslipidemia, has proven anticarcinogenic properties mediated by peroxisome proliferator-activated receptor-alpha (PPARα) agonism, thereby also influencing WNT-associated signaling molecules. MATERIALS AND METHODS: The antitumor apoptotic effect of fenofibrate at doses ranging from 0.1-200 µM was investigated on a total of seven human, two murine myeloma/lymphoma cell lines and two healthy control cell lines, as determined by 3'3-Dihexyloxacarbocyanine iodide (DiOC6) and propidium iodide (PI) staining in flow cytometry. RESULTS: Fenofibrate significantly reduced viability due to apoptosis induction in all investigated myeloma and lymphoma cell lines in a dose-dependent manner, whereas healthy control cells were less sensitive. CONCLUSION: Our results provide a rationale for future in vitro and in vivo studies with fenofibrate as a safe and well-tolerated agent in MM and lymphoma treatment.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Fenofibrato/farmacologia
Linfoma/tratamento farmacológico
Mieloma Múltiplo/tratamento farmacológico
[Mh] Termos MeSH secundário: Anticarcinógenos/farmacologia
Carbocianinas/administração & dosagem
Linhagem Celular Tumoral
Seres Humanos
Linfoma/metabolismo
Mieloma Múltiplo/metabolismo
Coloração e Rotulagem/métodos
Via de Sinalização Wnt/efeitos dos fármacos
beta Catenina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anticarcinogenic Agents); 0 (Carbocyanines); 0 (beta Catenin); 54501-79-0 (3,3'-dihexyl-2,2'-oxacarbocyanine); U202363UOS (Fenofibrate)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170703
[St] Status:MEDLINE



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