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Pesquisa : D02.455.426.392.368 [Categoria DeCS]
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[PMID]:29202411
[Au] Autor:Teixeira RG; Brás AR; Côrte-Real L; Tatikonda R; Sanches A; Robalo MP; Avecilla F; Moreira T; Garcia MH; Haukka M; Preto A; Valente A
[Ad] Endereço:Centro de Química Estrutural, Faculdade de Ciências da Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
[Ti] Título:Novel ruthenium methylcyclopentadienyl complex bearing a bipyridine perfluorinated ligand shows strong activity towards colorectal cancer cells.
[So] Source:Eur J Med Chem;143:503-514, 2018 Jan 01.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Three new compounds have been synthesized and completely characterized by analytical and spectroscopic techniques. The new bipyridine-perfluorinated ligand L1 and the new organometallic complex [Ru(η -MeCp)(PPh ) Cl] (Ru1) crystalize in the centrosymmetric triclinic space group P1¯. Analysis of the phenotypic effects induced by both organometallic complexes Ru1 and [Ru(η -MeCp)(PPh )(L1)][CF SO ] (Ru2), on human colorectal cancer cells (SW480 and RKO) survival, showed that Ru2 has a potent anti-proliferative activity, 4-6 times higher than cisplatin, and induce apoptosis in these cells. Data obtained in a noncancerous cell line derived from normal colon epithelial cells (NCM460) revealed an intrinsic selectivity of Ru2 for malignant cells at low concentrations, showing the high potential of this compound as a selective anticancer agent.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Neoplasias Colorretais/tratamento farmacológico
Cicloparafinas/farmacologia
Compostos Organometálicos/farmacologia
Piridinas/farmacologia
Rutênio/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/síntese química
Antineoplásicos/química
Apoptose/efeitos dos fármacos
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Neoplasias Colorretais/patologia
Cicloparafinas/química
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Seres Humanos
Ligantes
Estrutura Molecular
Compostos Organometálicos/síntese química
Compostos Organometálicos/química
Piridinas/química
Rutênio/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Cycloparaffins); 0 (Ligands); 0 (Organometallic Compounds); 0 (Pyridines); 26519-91-5 (methylcyclopentadiene); 7UI0TKC3U5 (Ruthenium)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180110
[Lr] Data última revisão:
180110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


  2 / 895 MEDLINE  
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[PMID]:28466286
[Au] Autor:Puza S; Gencturk E; Odabasi IE; Iseri E; Mutlu S; Ulgen KO
[Ad] Endereço:Department of Chemical Engineering, Biosystems Engineering Laboratory, Bogazici University, 34342, Istanbul, Turkey.
[Ti] Título:Fabrication of cyclo olefin polymer microfluidic devices for trapping and culturing of yeast cells.
[So] Source:Biomed Microdevices;19(2):40, 2017 Jun.
[Is] ISSN:1572-8781
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A microfluidic platform is designed and fabricated to investigate the role of uncharacterized YOR060C (Sld7) protein in aging in yeast cells for the first time. Saccharomyces cerevisiae yeast cells are trapped in the series of C-shaped regions (0.5 nL) of COP (cyclo olefin polymer), PMMA (poly methylmethacrylate), or PS (polystyrene) microbioreactors. The devices are fabricated using hot embossing and thermo-compression bonding methods. Photolithography and electrochemical etching are used to form the steel mold needed for hot embossing. The cell cycle processes are investigated by monitoring green fluorescent protein (GFP) tagged Sld7 expressions under normal as well as calorie restricted conditions. The cells are loaded at 1 µL/min flowrate and trapped successfully within each chamber. The medium is continuously fed at 0.1 µL/min throughout the experiments. Fluorescent signals of the low abundant Sld7 proteins could be distinguished only on COP devices. The background fluorescence of COP is found 1.22 and 7.24 times lower than that of PMMA, and PS, respectively. Hence, experiments are continued with COP, and lasted for more than 40 h without any contamination. The doubling time of the yeast cells are found as 72 min and 150 min, and the growth rates as 9.63 × 10 min and 4.62 × 10 min , in 2% glucose containing YPD and YNB medium, respectively. The product concentration (Sld7p:GFP) increased in accordance with cell growth. The dual role of Sld7 protein in both cell cycle and chronological aging needs to be further investigated following the preliminary experimental results.
[Mh] Termos MeSH primário: Técnicas de Cultura de Células/instrumentação
Separação Celular/instrumentação
Células Imobilizadas/citologia
Cicloparafinas/química
Dispositivos Lab-On-A-Chip
Polímeros/química
Saccharomyces cerevisiae/citologia
[Mh] Termos MeSH secundário: Reatores Biológicos
Simulação por Computador
Desenho de Equipamento
Hidrodinâmica
Microscopia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cycloparaffins); 0 (Polymers)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171225
[Lr] Data última revisão:
171225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1007/s10544-017-0182-3


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[PMID]:28242052
[Au] Autor:Wouters B; Dapic I; Valkenburg TS; Wouters S; Niezen L; Eeltink S; Corthals GL; Schoenmakers PJ
[Ad] Endereço:University of Amsterdam, van't Hoff Institute for Molecular Sciences, Analytical-Chemistry Group, Science Park 904, 1098 XH Amsterdam, The Netherlands; TI-COAST, Science Park 904, 1098 XH Amsterdam, The Netherlands. Electronic address: b.wouters@uva.nl.
[Ti] Título:A cyclic-olefin-copolymer microfluidic immobilized-enzyme reactor for rapid digestion of proteins from dried blood spots.
[So] Source:J Chromatogr A;1491:36-42, 2017 Mar 31.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A critical step in the bottom-up characterization of proteomes is the conversion of proteins to peptides, by means of endoprotease digestion. Nowadays this method typically uses overnight digestion and as such represents a considerable bottleneck for high-throughput analysis. This report describes protein digestion using an immobilized-enzyme reactor (IMER), which enables accelerated digestion times that are completed within seconds to minutes. For rapid digestion to occur, a cyclic-olefin-copolymer microfluidic reactor was constructed containing trypsin immobilized on a polymer monolithic material through a 2-vinyl-4,4-dimethylazlactone linker. The IMER was applied for the rapid offline digestion of both singular protein standards and a complex protein mixture prior to liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS/MS) analysis. The effects of protein concentration and residence time in the IMER were assessed for protein standards of varying molecular weight between 11 and 240kDa. Compared to traditional in-solution digestion, IMER-facilitated protein digestion at room temperature for 5min yielded similar results in terms of sequence coverage and number of identified peptides. Good repeatability was demonstrated with a relative standard deviation of 6% for protein-sequence coverage. The potential of the IMER was also demonstrated for a complex protein mixture in the analysis of dried blood spots. Compared to a traditional workflow a similar number of proteins could be identified, while reducing the total analysis time from 22.5h to 4h and importantly omitting the sample-pre-treatment steps (denaturation, reduction, and alkylation). The identified proteins from two workflows showed similar distributions in terms of molecular weight and hydrophobic character.
[Mh] Termos MeSH primário: Proteínas Sanguíneas/análise
Cicloparafinas/química
Teste em Amostras de Sangue Seco
Enzimas Imobilizadas
Técnicas Analíticas Microfluídicas/instrumentação
Polímeros/química
[Mh] Termos MeSH secundário: Teste em Amostras de Sangue Seco/instrumentação
Teste em Amostras de Sangue Seco/métodos
Enzimas Imobilizadas/química
Enzimas Imobilizadas/metabolismo
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Proteins); 0 (Cycloparaffins); 0 (Enzymes, Immobilized); 0 (Polymers)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170516
[Lr] Data última revisão:
170516
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE


  4 / 895 MEDLINE  
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[PMID]:28198111
[Au] Autor:Song X; Yu L; Shiono T; Hasan T; Cai Z
[Ad] Endereço:State Key Laboratory for Modification of Chemical Fibers and Polymer Materials and College of Material Science and Engineering, Donghua University, Shanghai, 201620, China.
[Ti] Título:Synthesis of Hydroxy-Functionalized Cyclic Olefin Copolymer and Its Block Copolymers with Semicrystalline Polyolefin Segments.
[So] Source:Macromol Rapid Commun;38(7), 2017 Apr.
[Is] ISSN:1521-3927
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Synthesis of hydroxy-functionalized cyclic olefin copolymer (COC) is achieved with remarkably high activity (up to 5.96 × 10 g-polymer mol-Ti h ) and controlled hydroxy group in a wide range (≈17.1 mol%) by using ansa-dimethylsilylene (fluorenyl)(amido)titanium complex. The catalyst also promotes living/controlled copolymerization to afford novel diblock copolymers consisting of hydroxy-functionalized COC and semicrystalline polyolefin sequence such as polyethylene and syndiotactic polypropylene, where the glass transition temperature of the norbornene/10-undecen-1-ol segment and each block length are controlled by comonomer composition and copolymerization time, respectively.
[Mh] Termos MeSH primário: Cicloparafinas/síntese química
Polímeros/síntese química
[Mh] Termos MeSH secundário: Catálise
Cicloparafinas/química
Estrutura Molecular
Compostos Organometálicos/química
Polimerização
Polímeros/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cycloparaffins); 0 (Organometallic Compounds); 0 (Polymers)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170216
[St] Status:MEDLINE
[do] DOI:10.1002/marc.201600815


  5 / 895 MEDLINE  
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[PMID]:28110334
[Au] Autor:Liu S; Cheng Y; Rao M; Tang M; Dong Z
[Ad] Endereço:College of Pharmacy, Chongqing Medical University, Chongqing, China.
[Ti] Título:Muscone Induces CYP1A2 and CYP3A4 Enzyme Expression in L02 Human Liver Cells and CYP1A2 and CYP3A11 Enzyme Expression in Kunming Mice.
[So] Source:Pharmacology;99(5-6):205-215, 2017.
[Is] ISSN:1423-0313
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:AIM: To examine the effect of synthetic muscone on the expression of CYP1A2 and CYP3A4 enzymes in human liver L02 cells and in the liver tissue of Kunming mice. METHODS: The L02 hepatic cell line was used to study the effect of low (10-4 µmol/L), middle (10-3 µmol/L), and high concentrations (10-2 µmol/L) of muscone on the expression of CYP1A2 and CYP3A4 enzymes. In addition, the cytochrome P450 (CYP) expression was investigated in Kunming mice after the administration of 10 mg/kg (low), 50 mg/kg (middle), and 100 mg/kg (high) dose of muscone for 6 days. A mixture of phenobarbital (30 mg/kg) and ß-napthoflavone (80 mg/kg) was used as positive control and the effects of the compounds on CYP expression were investigated at the end of 6- and 12-day periods. RESULTS: Muscone induced the expression of CYP1A2 (middle and low concentrations) and of CYP3A4 (high concentration) enzymes in L02 cells. In vivo, administration of muscone in Kunming mice revealed significant weight reduction at the end of 6- and 12-day periods (middle and high doses, respectively), compared to the control group (p < 0.05). Liver toxicity scores indicated that the liver injuries in the positive control and high doses of muscone group were significantly higher in the 6- and 12-day periods, compared to those in the blank control group (p < 0.05). Furthermore, muscone induced CYP1A2 and CYP3A11 expressions in Kunming mice at the middle dose and all doses during the 12-day period as demonstrated by immunoblotting experiments. A low dose of mucone induced the CYP enzyme expression more rapidly, whereas a high dose of muscone caused the longest inductive effect. The results were confirmed by immunohistochemistry experiments and real-time PCR studies, where similar patterns of muscone-mediated inductive effects were noted. CONCLUSIONS: Muscone induces CYP1A2 and CYP3A4 expression in liver cells in vitro and in vivo. In addition, it exhibits liver toxicity in Kunming mice at concentrations higher than 50 mg/kg. The CYP-inductive effect that is caused by muscone encompasses a 6- to 12-day period of activity after drug administration as demonstrated by follow-up in vivo studies.
[Mh] Termos MeSH primário: Cicloparafinas/farmacologia
Citocromo P-450 CYP1A2/biossíntese
Citocromo P-450 CYP3A/biossíntese
Hepatócitos/efeitos dos fármacos
Hepatócitos/enzimologia
Proteínas de Membrana/biossíntese
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Indutores do Citocromo P-450 CYP1A2/farmacologia
Indutores do Citocromo P-450 CYP3A/farmacologia
Relação Dose-Resposta a Droga
Expressão Gênica/efeitos dos fármacos
Seres Humanos
Masculino
Camundongos
Fenobarbital/farmacologia
beta-Naftoflavona/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cycloparaffins); 0 (Cytochrome P-450 CYP1A2 Inducers); 0 (Cytochrome P-450 CYP3A Inducers); 0 (Membrane Proteins); 6051-87-2 (beta-Naphthoflavone); EC 1.14.13.67 (CYP3A4 protein, human); EC 1.14.14.1 (CYP1A2 protein, human); EC 1.14.14.1 (Cyp3a11 protein, mouse); EC 1.14.14.1 (Cytochrome P-450 CYP1A2); EC 1.14.14.1 (Cytochrome P-450 CYP3A); UPS3C6CV36 (muscone); YQE403BP4D (Phenobarbital)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170123
[St] Status:MEDLINE
[do] DOI:10.1159/000455154


  6 / 895 MEDLINE  
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[PMID]:28052535
[Au] Autor:Li S; Chen F; Li N; Wang W; Sheng X; Wang A; Cong Y; Wang X; Zhang T
[Ad] Endereço:State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, P. R. China.
[Ti] Título:Synthesis of Renewable Triketones, Diketones, and Jet-Fuel Range Cycloalkanes with 5-Hydroxymethylfurfural and Ketones.
[So] Source:ChemSusChem;10(4):711-719, 2017 Feb 22.
[Is] ISSN:1864-564X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A series of renewable C -C triketones with repeating [COCH CH ] units were synthesized in high carbon yields (ca. 90 %) by the aqueous-phase hydrogenation of the aldol-condensation products of 5-hydroxylmethylfurfural (HMF) and ketones over an Au/TiO catalyst. Compared with the reported routes, this new route has many advantages such as being environmentally friendly, having fewer steps, using a cheaper and reusable catalyst, etc. The triketones as obtained can be used as feedstocks in the production of conducting or semi-conducting polymers. Through a solvent-free intramolecular aldol condensation over solid-base catalysts, the triketones were selectively converted to diketones, which can be used as intermediates in the synthesis of useful chemicals or polymers. As another application, the tri- and diketones can also be utilized as precursors for the synthesis of jet-fuel range branched cycloalkanes with low freezing points (224-248 K) and high densities (ca. 0.81 g mL ).
[Mh] Termos MeSH primário: Cicloparafinas/síntese química
Furaldeído/análogos & derivados
Química Verde/métodos
Cetonas/síntese química
[Mh] Termos MeSH secundário: Catálise
Furaldeído/química
Hidrocarbonetos/química
Hidrogenação
Cetonas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cycloparaffins); 0 (Hydrocarbons); 0 (Ketones); 70ETD81LF0 (5-hydroxymethylfurfural); 8008-20-6 (JP5 jet fuel); DJ1HGI319P (Furaldehyde)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170105
[St] Status:MEDLINE
[do] DOI:10.1002/cssc.201601727


  7 / 895 MEDLINE  
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[PMID]:27873475
[Au] Autor:Wu L; Mascal M; Farmer TJ; Arnaud SP; Wong Chang MA
[Ad] Endereço:Department of Chemistry, University of California Davis, 1 Shields Avenue, Davis, CA, 95616, USA.
[Ti] Título:Electrochemical Coupling of Biomass-Derived Acids: New C Platforms for Renewable Polymers and Fuels.
[So] Source:ChemSusChem;10(1):166-170, 2017 Jan 10.
[Is] ISSN:1864-564X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Electrolysis of biomass-derived carbonyl compounds is an alternative to condensation chemistry for supplying products with chain length >C for biofuels and renewable materials production. Kolbe coupling of biomass-derived levulinic acid is used to obtain 2,7-octanedione, a new platform molecule only two low process-intensity steps removed from raw biomass. Hydrogenation to 2,7-octanediol provides a chiral secondary diol largely unknown to polymer chemistry, whereas intramolecular aldol condensation followed by hydrogenation yields branched cycloalkanes suitable for use as high-octane, cellulosic gasoline. Analogous electrolysis of an itaconic acid-derived methylsuccinic monoester yields a chiral 2,5-dimethyladipic acid diester, another underutilized monomer owing to lack of availability.
[Mh] Termos MeSH primário: Biocombustíveis
Biomassa
Ácidos Levulínicos/química
Polímeros/química
[Mh] Termos MeSH secundário: Aldeídos/química
Catálise
Cicloparafinas/química
Eletroquímica
Cetonas/química
Poliésteres/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aldehydes); 0 (Biofuels); 0 (Cycloparaffins); 0 (Ketones); 0 (Levulinic Acids); 0 (Polyesters); 0 (Polymers); RYX5QG61EI (levulinic acid)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161123
[St] Status:MEDLINE
[do] DOI:10.1002/cssc.201601271


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[PMID]:27491233
[Au] Autor:Jiang T; Huang LF; Zhou SJ; Cui JJ; Ye Q
[Ti] Título:[Brain Protection of Muscone in Rats with Brain Injury].
[So] Source:Zhongguo Zhong Xi Yi Jie He Za Zhi;36(6):724-8, 2016 Jun.
[Is] ISSN:1003-5370
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To observe cerebral protective effect of muscone (nasal administration) on traumatic brain injury model rats. METHODS: SD rats were divided into the sham-operation group, the model group, and the treatment groups according to random digit table, 50 in each group. Traumatic brain injury model was established by controlled cortical strike. Rats in the sham-operation group received surgery and anesthesia procedures only, with no strike. Muscone (1.8 mg/kg) was delivered to rats in the treatment group using in situ nasal perfusion, 30 min each time, twice daily for 7 successive days. Water content of brain tissue was detected in each group before intervention (T1), at day 3 of intervention (T2), day 5 of intervention (T3), and after intervention (T4), respectively. Expression levels of brain derived neurotrophic factor (BDNF) and nerve growth factor (NGF) were detected using immunohistochemical analysis. RESULTS: Compared with the sham-operated group, water content of brain tissue increased (P < 0.05), and expression levels of NGF and BDNF decreased in the model group at T1, T2, T3, and T4 (P <0. 01). Compared with the model group, water content of brain tissue decreased (P < 0.05), and expression levels of NGF and BDNF increased (P < 0.01) in the treatment group at T1, T2, and T3. CONCLUSION: Nasal administration of muscone could reduce water content of brain tissue, alleviate cerebral edema, promote secretion of BDNF and NGF by olfactory ensheathing cells in traumatic brain injury rats.
[Mh] Termos MeSH primário: Lesões Encefálicas/tratamento farmacológico
Cicloparafinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Encéfalo/efeitos dos fármacos
Fator Neurotrófico Derivado do Encéfalo/metabolismo
Fator de Crescimento Neural/metabolismo
Distribuição Aleatória
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Brain-Derived Neurotrophic Factor); 0 (Cycloparaffins); 9061-61-4 (Nerve Growth Factor); UPS3C6CV36 (muscone)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160806
[St] Status:MEDLINE


  9 / 895 MEDLINE  
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[PMID]:27490022
[Au] Autor:Liu C; Luo C; Hao L; Wu Q; Xie H; Zhao S; Hao C; Zhao D; Cheng M
[Ad] Endereço:Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, PR China.
[Ti] Título:Design, synthesis and biological evaluation of novel cholesteryl ester transfer protein inhibitors bearing a cycloalkene scaffold.
[So] Source:Eur J Med Chem;123:419-430, 2016 Nov 10.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Cholesteryl ester transfer protein (CETP) is a potential target for cardiovascular disease therapy as inhibition of CETP leads to increased HDL-C in humans. Based on the structure of Merck's biphenyl CETP inhibitor, we designed novel N,N-substituted-cycloalkenyl-methylamine scaffold derivatives by utilizing core replacement and conformational restriction strategies. Consequently, twenty-eight compounds were synthesized and evaluated for their inhibitory activity against CETP. Their preliminary structure-activity relationships (SARs) studies indicate that polar substituents were tolerated in moiety A and hydrophobic alkyl groups at the 5-position of cyclohexene were critical for potency. Among them, compound 17a, bearing an N-(5-pyrazolyl-pyrimidin-2-yl)-cycloalkenyl- methylamine scaffold, exhibited excellent CETP inhibitory activity (IC50 = 0.07 µM) in vitro. Furthermore, it showed an acceptable pharmacokinetic profile in S-D rats and efficient HDL-C increase in high-fat fed hamsters.
[Mh] Termos MeSH primário: Proteínas de Transferência de Ésteres de Colesterol/antagonistas & inibidores
Cicloparafinas/síntese química
Cicloparafinas/farmacologia
Desenho de Drogas
[Mh] Termos MeSH secundário: Animais
Técnicas de Química Sintética
Cricetinae
Cicloparafinas/química
Cicloparafinas/farmacocinética
Dieta Hiperlipídica/efeitos adversos
Masculino
Ratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholesterol Ester Transfer Proteins); 0 (Cycloparaffins)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160805
[St] Status:MEDLINE


  10 / 895 MEDLINE  
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[PMID]:27371017
[Au] Autor:Wei X; Sun P; Yang S; Zhao L; Wu J; Li F; Pu Q
[Ad] Endereço:State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province, Department of Chemistry, Lanzhou University, Lanzhou, Gansu, 730000, China; Department of Chemistry, Tonghua Normal University, Tonghua, Jilin, 134002, China.
[Ti] Título:Microchip electrophoresis with background electrolyte containing polyacrylic acid and high content organic solvent in cyclic olefin copolymer microchips for easily adsorbed dyes.
[So] Source:J Chromatogr A;1457:144-50, 2016 Jul 29.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Plastic microchips can significantly reduce the fabrication cost but the adsorption of some analytes limits their application. In this work, background electrolyte containing ionic polymer and high content of organic solvent was adopted to eliminate the analyte adsorption and achieve highly efficient separation in microchip electrophoresis. Two dyes, rhodamine 6G (Rh6G) and rhodamine B (RhB) were used as the model analytes. By using methanol as the organic solvent and polyacrylic acid (PAA) as a multifunctional additive, successful separation of the two dyes within 75µm id. microchannels was realized. The role of PAA is multiple, including viscosity regulator, selectivity modifier and active additive for counteracting analyte adsorption on the microchannel surface. The number of theoretical plate of 7.0×10(5)/m was attained within an effective separation distance of 2cm using background electrolyte consisting 80% methanol, 0.36% PAA and 30mmol/L phosphate at pH 5.0. Under optimized conditions, relative standard deviations of Rh6G and RhB detection (n=5) were no more than 1.5% for migration time and 2.0% for peak area, respectively. The limit of detection (S/N=3) was 0.1nmol/L for Rh6G. The proposed technique was applied in the determination of both Rh6G and RhB in chilli powder and lipstick samples with satisfactory recoveries of 81.3-103.7%.
[Mh] Termos MeSH primário: Corantes/análise
Cicloparafinas/química
[Mh] Termos MeSH secundário: Resinas Acrílicas
Adsorção
Cosméticos/análise
Eletroforese em Microchip/instrumentação
Eletroforese em Microchip/métodos
Análise de Alimentos
Metanol
Polieletrólitos
Rodaminas/análise
Solventes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acrylic Resins); 0 (Coloring Agents); 0 (Cosmetics); 0 (Cycloparaffins); 0 (Polyelectrolytes); 0 (Rhodamines); 0 (Solvents); 037VRW83CF (rhodamine 6G); 4Q93RCW27E (carbopol 940); K7G5SCF8IL (rhodamine B); Y4S76JWI15 (Methanol)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160703
[St] Status:MEDLINE



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