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[PMID]:29360846
[Au] Autor:Yu X; Stallone JN; Heaps CL; Han G
[Ad] Endereço:Veterinary Physiology & Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States of America.
[Ti] Título:The activation of G protein-coupled estrogen receptor induces relaxation via cAMP as well as potentiates contraction via EGFR transactivation in porcine coronary arteries.
[So] Source:PLoS One;13(1):e0191418, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Estrogen exerts protective effects against cardiovascular diseases in premenopausal women, but is associated with an increased risk of both coronary heart disease and stroke in older postmenopausal women. Studies have shown that activation of the G-protein-coupled estrogen receptor 1 (GPER) can cause either relaxation or contraction of arteries. It is highly likely that these dual actions of GPER may contribute to the seemingly paradoxical effects of estrogen in regulating coronary artery function. The objective of this study was to test the hypothesis that activation of GPER enhances agonist-stimulated porcine coronary artery contraction via epidermal growth factor receptor (EGFR) transactivation and its downstream extracellular signal-regulated kinases (ERK1/2) pathway. Isometric tension studies and western blot were performed to determine the effect of GPER activation on coronary artery contraction. Our findings demonstrated that G-1 caused concentration-dependent relaxation of ET-1-induced contraction, while pretreatment of arterial rings with G-1 significantly enhanced ET-1-induced contraction. GPER antagonist, G-36, significantly inhibited both the G-1-induced relaxation effect and G-1-enhanced ET-1 contraction. Gallein, a Gßγ inhibitor, significantly increased G-1-induced relaxation, yet inhibited G-1-enhanced ET-1-mediated contraction. Similarly, inhibition of EGFR with AG1478 or inhibition of Src with phosphatase 2 further increased G-1-induced relaxation responses in coronary arteries, but decreased G-1-enhanced ET-1-induced contraction. Western blot experiments in porcine coronary artery smooth muscle cells (PCASMC) showed that G-1 increased tyrosine phosphorylation of EGFR, which was inhibited by AG-1478. Furthermore, enzyme-linked immunosorbent assays showed that the level of heparin-binding EGF (HB-EGF) released by ET-1 treatment increased two-fold; whereas pre-incubation with G-1 further increased ET-1-induced HB-EGF release to four-fold over control conditions. Lastly, the role of ERK1/2 was determined by applying the MEK inhibitor, PD98059, in isometric tension studies and detecting phospho-ERK1/2 in immunoblotting. PD98059 potentiated G-1-induced relaxation response, but blocked G-1-enhanced ET-1-induced contraction. By western blot, G-1 treatment decreased phospho-ERK1/2, however, in the presence of the adenylyl cyclase inhibitor, SQ22536, G-1 significantly increased ERK1/2 phosphorylation in PCASMC. These data demonstrate that activation of GPER induces relaxation via cAMP as well as contraction via a mechanism involving transactivation of EGFR and the phosphorylation of ERK1/2 in porcine coronary arteries.
[Mh] Termos MeSH primário: Vasos Coronários/fisiologia
Receptor do Fator de Crescimento Epidérmico/genética
Receptores Estrogênicos/metabolismo
Receptores Acoplados a Proteínas-G/metabolismo
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Vasos Coronários/efeitos dos fármacos
Ciclopentanos/farmacologia
Flavonoides/farmacologia
Seres Humanos
Técnicas In Vitro
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Modelos Cardiovasculares
Miócitos de Músculo Liso/metabolismo
Quinazolinas/farmacologia
Quinolinas/farmacologia
Receptores Acoplados a Proteínas-G/agonistas
Suínos
Ativação Transcricional
Tirfostinas/farmacologia
Vasodilatação/efeitos dos fármacos
Vasodilatação/genética
Vasodilatação/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (1-(4-(6-bromobenzo(1,3)dioxol-5-yl)-3a,4,5,9b-tetrahydro-3H-cyclopenta(c)quinolin-8-yl)ethanone); 0 (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one); 0 (Cyclopentanes); 0 (Flavonoids); 0 (Quinazolines); 0 (Quinolines); 0 (Receptors, Estrogen); 0 (Receptors, G-Protein-Coupled); 0 (Tyrphostins); 170449-18-0 (tyrphostin AG 1478); EC 2.7.10.1 (Receptor, Epidermal Growth Factor)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191418


  2 / 7909 MEDLINE  
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[PMID]:29324342
[Au] Autor:Wang PC; Chiu DC; Jan JT; Huang WI; Tseng YC; Li TT; Cheng TJ; Tsai KC; Fang JM
[Ad] Endereço:Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
[Ti] Título:Peramivir conjugates as orally available agents against influenza H275Y mutant.
[So] Source:Eur J Med Chem;145:224-234, 2018 Feb 10.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Peramivir is an efficacious neuraminidase (NA) inhibitor for treatment of influenza by intravenous administration. However, the efficacy of peramivir toward the H275Y mutant is appreciably reduced. To address this drawback, conjugation of peramivir with caffeic acid is devised in this study to enhance the binding affinity with neuraminidases. The C2-OH group of peramivir is elaborated to link with caffeate derivatives, giving the desired conjugates 8 and 9 that possess potent NA inhibitory activity against both wild-type and H275Y viruses with the IC values in nanomolar range. The molecular modeling reveals that the caffeate moiety of conjugate 9 prefers to reside in the 295-cavity of H275Y neuraminidase, thus providing additional hydrogen bonds and hydrophobic interactions to compensate the reduced binding affinity of the peramivir moiety due to Glu-276 dislocation in H275Y mutant. In comparison with peramivir, the lipophilicity of conjugates 8 and 9 also increases by incorporation of the caffeate moiety. Thus, conjugates 8 and 9 offer better effect to protect MDCK cells from infection of H275Y virus with low EC value (∼17 nM). Administration of conjugates 8 or 9 by oral gavage is effective in treatment of mice that are infected by lethal dose of wild-type or H275Y influenza viruses. Considering drug metabolism, since the ester linkage in conjugate 8 is susceptible to hydrolysis in plasma, conjugate 9 with robust amide linkage may be a better candidate for development into orally available anti-influenza drug that is also active to mutant viruses.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Ciclopentanos/farmacologia
Guanidinas/farmacologia
Vírus da Influenza A/efeitos dos fármacos
[Mh] Termos MeSH secundário: Administração Oral
Animais
Antivirais/administração & dosagem
Antivirais/química
Ciclopentanos/administração & dosagem
Ciclopentanos/química
Cães
Relação Dose-Resposta a Droga
Guanidinas/administração & dosagem
Guanidinas/química
Células HEK293
Seres Humanos
Vírus da Influenza A/genética
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Testes de Sensibilidade Microbiana
Modelos Moleculares
Estrutura Molecular
Mutação
Coelhos
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Cyclopentanes); 0 (Guanidines); QW7Y7ZR15U (peramivir)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE


  3 / 7909 MEDLINE  
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[PMID]:29373575
[Au] Autor:Waqas M; Shahzad R; Hamayun M; Asaf S; Khan AL; Kang SM; Yun S; Kim KM; Lee IJ
[Ad] Endereço:School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea.
[Ti] Título:Biochar amendment changes jasmonic acid levels in two rice varieties and alters their resistance to herbivory.
[So] Source:PLoS One;13(1):e0191296, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Biochar addition to soil not only sequesters carbon for the long-term but enhances agricultural productivity. Several well-known benefits arise from biochar amendment, including constant provision of nutrients, increased soil moisture retention, decreased soil bulk density, and sometimes the induction of systemic resistance against foliar and soil borne plant pathogens. However, no research has investigated the potential of biochar to increase resistance against herbivory. The white-backed plant hopper (WBPH) (Sogatella furcifera Horváth) is a serious agricultural pest that targets rice (Oryza sativa L.), a staple crop that feeds half of the world's human population. Therefore, we investigated the (1) optimization of biochar amendment levels for two rice varieties ('Cheongcheong' and 'Nagdong') and (2) subsequent effects of different biochar amendments on resistance and susceptibility of these two varieties to WBPH infestation. Initial screening results for the optimization level revealed that the application of biochar 10% (w/w) to the rooting media significantly improved plant physiological characteristics of both rice varieties. However, levels of biochar amendment, mainly 1, 2, 3, and 20%, resulted in negative effects on plant growth characteristics. Cheongcheong and Nagdong rice plants grown with the optimum biochar level showed contrasting reactions to WBPH infestation. Specifically, biochar application significantly increased plant growth characteristics of Nagdong when exposed to WBPH infestation and significantly decreased these characteristics in Cheongcheong. The amount of WBPH-induced damage to plants was significantly lower and higher in Nagdong and Cheongcheong, respectively, compared to that in the controls. Higher levels of jasmonic acid caused by the biochar priming effect could have accumulated in response to WBPH infestation, resulting in a maladaptive response to stress, negatively affecting growth and resistance to WBPH in Cheongcheong. This study highlights the importance of investigating the effects of biochar on different rice varieties before application on a commercial scale to avoid potential crop losses.
[Mh] Termos MeSH primário: Carvão Vegetal/farmacologia
Ciclopentanos/metabolismo
Herbivoria
Oryza/efeitos dos fármacos
Oryza/metabolismo
Oxilipinas/metabolismo
[Mh] Termos MeSH secundário: Oryza/fisiologia
Solo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cyclopentanes); 0 (Oxylipins); 0 (Soil); 0 (biochar); 16291-96-6 (Charcoal); 6RI5N05OWW (jasmonic acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191296


  4 / 7909 MEDLINE  
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[PMID]:29304107
[Au] Autor:Zdyb A; Salgado MG; Demchenko KN; Brenner WG; Plaszczyca M; Stumpe M; Herrfurth C; Feussner I; Pawlowski K
[Ad] Endereço:Department of Ecology, Environment and Plant Sciences, Stockholm University, Stockholm, Sweden.
[Ti] Título:Allene oxide synthase, allene oxide cyclase and jasmonic acid levels in Lotus japonicus nodules.
[So] Source:PLoS One;13(1):e0190884, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Jasmonic acid (JA), its derivatives and its precursor cis-12-oxo phytodienoic acid (OPDA) form a group of phytohormones, the jasmonates, representing signal molecules involved in plant stress responses, in the defense against pathogens as well as in development. Elevated levels of JA have been shown to play a role in arbuscular mycorrhiza and in the induction of nitrogen-fixing root nodules. In this study, the gene families of two committed enzymes of the JA biosynthetic pathway, allene oxide synthase (AOS) and allene oxide cyclase (AOC), were characterized in the determinate nodule-forming model legume Lotus japonicus JA levels were to be analysed in the course of nodulation. Since in all L. japonicus organs examined, JA levels increased upon mechanical disturbance and wounding, an aeroponic culture system was established to allow for a quick harvest, followed by the analysis of JA levels in whole root and shoot systems. Nodulated plants were compared with non-nodulated plants grown on nitrate or ammonium as N source, respectively, over a five week-period. JA levels turned out to be more or less stable independently of the growth conditions. However, L. japonicus nodules formed on aeroponically grown plants often showed patches of cells with reduced bacteroid density, presumably a stress symptom. Immunolocalization using a heterologous antibody showed that the vascular systems of these nodules also seemed to contain less AOC protein than those of nodules of plants grown in perlite/vermiculite. Hence, aeroponically grown L. japonicus plants are likely to be habituated to stress which could have affected JA levels.
[Mh] Termos MeSH primário: Ciclopentanos/metabolismo
Fabaceae/fisiologia
Oxirredutases Intramoleculares/metabolismo
Loteae/metabolismo
Oxilipinas/metabolismo
Nodulação
[Mh] Termos MeSH secundário: Oxirredutases Intramoleculares/genética
Loteae/enzimologia
RNA Mensageiro/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Cyclopentanes); 0 (Oxylipins); 0 (RNA, Messenger); 6RI5N05OWW (jasmonic acid); EC 5.3.- (Intramolecular Oxidoreductases); EC 5.3.99.6 (hydroperoxide isomerase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190884


  5 / 7909 MEDLINE  
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[PMID]:29037700
[Au] Autor:D'Onofrio C; Matarese F; Cuzzola A
[Ad] Endereço:Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, I-56124 Pisa, Italy; Nutraceuticals and Food for Health - Nutrafood, University of Pisa, Via del Borghetto 80, I-56124 Pisa, Italy. Electronic address: claudio.donofrio@unipi.it.
[Ti] Título:Effect of methyl jasmonate on the aroma of Sangiovese grapes and wines.
[So] Source:Food Chem;242:352-361, 2018 Mar 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Methyl jasmonate (MeJA) was applied in a vineyard on leaves and grape clusters of cv Sangiovese to test its ability to stimulate the production of aromas and identify the main genes involved in the biosynthetic pathways switched on by the elicitor. MeJA application led to a delay in grape technological maturity and a significant increase in the concentration of several berry aroma classes (about twice the total aroma: from around 3 to 6µg/g of berry). Of these, monoterpenes showed the most significant increase. An analysis of the expression of terpenoid biosynthesis genes confirmed that the MeJA application activated the related biosynthetic pathway. The expression of all the TPS genes analyzedwas higher in samples treated with MeJA. Also the wines produced by microvinification of Sangiovese treated and untreated grapes showed a rise in the aroma concentration as in berries, with an important impact on longevity and sensorial characters of wines.
[Mh] Termos MeSH primário: Acetatos
Ciclopentanos
Manipulação de Alimentos/métodos
Frutas/química
Odorantes/análise
Oxilipinas
Vitis/química
Vinho/análise
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica de Plantas
Monoterpenos/análise
Monoterpenos/metabolismo
Vitis/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Cyclopentanes); 0 (Monoterpenes); 0 (Oxylipins); 900N171A0F (methyl jasmonate)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171018
[St] Status:MEDLINE


  6 / 7909 MEDLINE  
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[PMID]:29311512
[Au] Autor:Murakami N; Yoshida M; Yoshino T; Matsunaga S
[Ad] Endereço:Faculty of Pharmaceutical Sciences, Hokkaido University.
[Ti] Título:Synthesis of Fluorine-Containing 6-Arylpurine Derivatives via Cp*Co(III)-Catalyzed C-H Bond Activation.
[So] Source:Chem Pharm Bull (Tokyo);66(1):51-54, 2018.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Cp*Co(III)-catalyzed (Cp*=pentamethylcyclopentadienyl) C-H bond functionalization of 6-arylpurines using gem-difluoroalkenes and allyl fluorides is described. The reaction with gem-difluoroalkenes afforded monofluoroalkenes with high (Z)-selectivity, while the reaction with allyl fluorides led to C-H allylation in moderate (Z)-selectivity. Both reactions proceeded using a user-friendly single-component catalyst [Cp*Co(CH CN) ](SbF ) in fluorinated alcohol solvents without any additives. Robustness was also demonstrated by a preparative-scale reaction under air.
[Mh] Termos MeSH primário: Cobalto/química
Ciclopentanos/química
Flúor/química
Compostos Organometálicos/química
Purinas/síntese química
[Mh] Termos MeSH secundário: Catálise
Estrutura Molecular
Purinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclopentanes); 0 (Organometallic Compounds); 0 (Purines); 284SYP0193 (Fluorine); 3G0H8C9362 (Cobalt); W60KTZ3IZY (purine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c17-00797


  7 / 7909 MEDLINE  
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[PMID]:29241316
[Au] Autor:Zlotek U
[Ad] Endereço:Department of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Poland.
[Ti] Título:Effect of jasmonic acid and yeast extract elicitation on low-molecular antioxidants and antioxidant activity of marjoram (Origanum majorana L.).
[So] Source:Acta Sci Pol Technol Aliment;16(4):371-377, 2017 Oct-Dec.
[Is] ISSN:1898-9594
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Elicitation, which is a way of inducing plant secondary metabolism, may be an effective method for improving the quality of plant food. The aim of this study was to determine how the application of jasmonic acid (as an abiotic elicitor) and yeast extract (as a biotic elicitor) influences the production of some bioactive compounds in marjoram and the antioxidant activity of this herb. METHODS: Elicitation with 0.01 µM and 1 µM jasmonic acid as well as 0.1% and 1% yeast extracts was used for improving the health-benefiting quality of marjoram. The study focused on the effects of eliciting the level of some phytochemicals and the antioxidant activity of marjoram. RESULTS: There were no significant differences in total phenolic content between the elicited and control plants. In turn, the elicitation with 0.1% and 1% yeast extracts caused 1.8- and 2.5-fold increases in the ascorbic acid content in marjoram leaves, respectively. Both biotic and abiotic elicitation resulted in elevation of chlorophyll content, but only the abiotic elicitor (jasmonic acid) caused a significant increase (by over 50%) in the carotenoid content of marjoram leaves. The antiradical activity of marjoram was increased by the abiotic and biotic elicitation, whereas only the abiotic elicitation resulted in improving the reducing power of this herb. CONCLUSIONS: In conclusion, biotic and abiotic elicitation could be an effective strategy for improving the level of some phytochemicals, as well as the antioxidant activity of marjoram. A particularly valuable finding obtained in this study is that natural elicitors e.g. yeast extract can be equally effective in elevating the content of some bioactive compounds in herbs e.g. marjoram as an abiotic one.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Ciclopentanos/farmacologia
Origanum/efeitos dos fármacos
Origanum/metabolismo
Oxilipinas/farmacologia
Saccharomyces cerevisiae/química
[Mh] Termos MeSH secundário: Ciclopentanos/administração & dosagem
Relação Dose-Resposta a Droga
Origanum/química
Oxilipinas/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Cyclopentanes); 0 (Oxylipins); 6RI5N05OWW (jasmonic acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.17306/J.AFS.0505


  8 / 7909 MEDLINE  
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[PMID]:29236385
[Au] Autor:Babenko LM; Shcherbatiuk MM; Skaterna TD; Kosakivska IV
[Ti] Título:Lipoxygenases and their metabolites in formation of plant stress tolerance.
[So] Source:Ukr Biochem J;89(1):5-21, 2017 Jan-Feb.
[Is] ISSN:2409-4943
[Cp] País de publicação:Ukraine
[La] Idioma:eng
[Ab] Resumo:The review focuses on the analysis of new information concerning molecular enzymology of lipoxygenases ­ proteins involved in lipid peroxidation and found in animals and plants. Modern concept of structural features, catalytic characteristics and functions of lipoxygenase family enzymes as well as products of their catalytic activity in plants have been discussed and summarized. Issues of enzyme localization in plant cells and tissues, evolution and distribution of lipoxygenases, involvement in production of signaling substances involved in formation of adaptation response to abiotic and biotic stress factors and in regulation of lipoxygenase signal system activity are highlighted. Participants of the elements signaling of LOX-pathway reception and transduction into genome are considered. Special attention is given to jasmonates, metabolites of the allene oxide synthase branch of the lipoxygenase cascade, because these metabolites have high biological activity, are ubiquitously present in all plant organisms, and are involved in regulation of vitally important processes. Data concerning lipoxygenase phylogeny, possible occurrence of a common predecessor for modern isoforms of the enzyme in pro- and eukaryote have been examined. Some results of our studies that open up possibilities of using the lipoxygenase catalytic activity characteristics as biological markers in plant stress tolerance researches are given.
[Mh] Termos MeSH primário: Adaptação Fisiológica/genética
Regulação da Expressão Gênica de Plantas
Genoma de Planta
Lipoxigenases/genética
Proteínas de Plantas/genética
Plantas/genética
[Mh] Termos MeSH secundário: Evolução Biológica
Ciclopentanos/metabolismo
Oxirredutases Intramoleculares/genética
Oxirredutases Intramoleculares/metabolismo
Lipoxigenases/metabolismo
Redes e Vias Metabólicas/genética
Oxilipinas/metabolismo
Filogenia
Proteínas de Plantas/metabolismo
Plantas/classificação
Plantas/enzimologia
Transdução de Sinais
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cyclopentanes); 0 (Oxylipins); 0 (Plant Proteins); 6RI5N05OWW (jasmonic acid); EC 1.13.11.- (Lipoxygenases); EC 5.3.- (Intramolecular Oxidoreductases); EC 5.3.99.6 (hydroperoxide isomerase)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.15407/ubj89.01.005


  9 / 7909 MEDLINE  
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[PMID]:28450397
[Au] Autor:Gonzalez de Valdivia E; Broselid S; Kahn R; Olde B; Leeb-Lundberg LMF
[Ad] Endereço:From the Departments of Experimental Medical Science.
[Ti] Título:G protein-coupled estrogen receptor 1 (GPER1)/GPR30 increases ERK1/2 activity through PDZ motif-dependent and -independent mechanisms.
[So] Source:J Biol Chem;292(24):9932-9943, 2017 06 16.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:G protein-coupled receptor 30 (GPR30), also called G protein-coupled estrogen receptor 1 (GPER1), is thought to play important roles in breast cancer and cardiometabolic regulation, but many questions remain about ligand activation, effector coupling, and subcellular localization. We showed recently that GPR30 interacts through the C-terminal type I PDZ motif with SAP97 and protein kinase A (PKA)-anchoring protein (AKAP) 5, which anchor the receptor in the plasma membrane and mediate an apparently constitutive decrease in cAMP production independently of G Here, we show that GPR30 also constitutively increases ERK1/2 activity. Removing the receptor PDZ motif or knocking down specifically AKAP5 inhibited the increase, showing that this increase also requires the PDZ interaction. However, the increase was inhibited by pertussis toxin as well as by wortmannin but not by AG1478, indicating that G and phosphoinositide 3-kinase (PI3K) mediate the increase independently of epidermal growth factor receptor transactivation. FK506 and okadaic acid also inhibited the increase, implying that a protein phosphatase is involved. The proposed GPR30 agonist G-1 also increased ERK1/2 activity, but this increase was only observed at a level of receptor expression below that required for the constitutive increase. Furthermore, deleting the PDZ motif did not inhibit the G-1-stimulated increase. Based on these results, we propose that GPR30 increases ERK1/2 activity via two G -mediated mechanisms, a PDZ-dependent, apparently constitutive mechanism and a PDZ-independent G-1-stimulated mechanism.
[Mh] Termos MeSH primário: Subunidades alfa Gi-Go de Proteínas de Ligação ao GTP/agonistas
Sistema de Sinalização das MAP Quinases
Proteína Quinase 1 Ativada por Mitógeno/metabolismo
Proteína Quinase 3 Ativada por Mitógeno/metabolismo
Modelos Moleculares
Fosfatidilinositol 3-Quinase/metabolismo
Receptores Estrogênicos/metabolismo
Receptores Acoplados a Proteínas-G/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Ancoragem à Quinase A/antagonistas & inibidores
Proteínas de Ancoragem à Quinase A/genética
Proteínas de Ancoragem à Quinase A/metabolismo
Substituição de Aminoácidos
Ciclopentanos/farmacologia
Ativação Enzimática/efeitos dos fármacos
Inibidores Enzimáticos/farmacologia
Subunidades alfa Gi-Go de Proteínas de Ligação ao GTP/química
Subunidades alfa Gi-Go de Proteínas de Ligação ao GTP/genética
Subunidades alfa Gi-Go de Proteínas de Ligação ao GTP/metabolismo
Células HEK293
Seres Humanos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Proteína Quinase 1 Ativada por Mitógeno/química
Proteína Quinase 1 Ativada por Mitógeno/genética
Proteína Quinase 3 Ativada por Mitógeno/química
Proteína Quinase 3 Ativada por Mitógeno/genética
Mutação
Domínios PDZ
Fosfatidilinositol 3-Quinase/química
Fosfatidilinositol 3-Quinase/genética
Fosforilação/efeitos dos fármacos
Processamento de Proteína Pós-Traducional/efeitos dos fármacos
Quinolinas/farmacologia
Interferência de RNA
Ensaio Radioligante
Receptores Estrogênicos/química
Receptores Estrogênicos/genética
Receptores Acoplados a Proteínas-G/agonistas
Receptores Acoplados a Proteínas-G/química
Receptores Acoplados a Proteínas-G/genética
Proteínas Recombinantes de Fusão/química
Proteínas Recombinantes de Fusão/metabolismo
Regulação para Cima/efeitos dos fármacos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (1-(4-(6-bromobenzo(1,3)dioxol-5-yl)-3a,4,5,9b-tetrahydro-3H-cyclopenta(c)quinolin-8-yl)ethanone); 0 (A Kinase Anchor Proteins); 0 (AKAP5 protein, human); 0 (Cyclopentanes); 0 (Enzyme Inhibitors); 0 (GPER protein, human); 0 (Quinolines); 0 (Receptors, Estrogen); 0 (Receptors, G-Protein-Coupled); 0 (Recombinant Fusion Proteins); EC 2.7.1.137 (Phosphatidylinositol 3-Kinase); EC 2.7.11.24 (MAPK1 protein, human); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3); EC 3.6.5.1 (GTP-Binding Protein alpha Subunits, Gi-Go)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M116.765875


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[PMID]:29216498
[Au] Autor:Zhao P; Wang L; Yin H
[Ad] Endereço:Key Laboratory of Stress Physiology and Ecology in Cold and Arid Regions, Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China; Shapotou Desert Research & Experiment Station, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, 730000, China. Electronic address: zhaopengshan@lzb.ac.cn.
[Ti] Título:Transcriptional responses to phosphate starvation in Brachypodium distachyon roots.
[So] Source:Plant Physiol Biochem;122:113-120, 2018 Jan.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Brachypodium distachyon is a model plant that has recently emerged in grass research. Although the growth and photochemical efficiency of this species respond strongly to phosphate (Pi) availability, its Pi starvation response mechanism, which controls the Pi homeostasis, remains largely unknown. This study presents the transcriptomic response profiles of Pi-deficient roots at growth stages during which the plants were starved but obvious growth defects were absent. The results identify several phosphate transporters (i.e., PHO1), purple acid phosphatases, and SYG1/PHO81/XPR1 (SPX) domain-containing proteins out of a total of 1740 differentially expressed genes (DEGs). In particular, the transcription factor ethylene response factor (ERF), basic helix-loop-helix (bHLH), and WRKY family genes were the three most abundant DEG groups and the latter was significantly enriched. Comparative transcriptome analysis of Brachypodium versus Arabidopsis and rice revealed the presence of several common components in response to Pi fluctuations. Most significantly, jasmonic acid (JA) signaling-related genes were overrepresented in gene ontology (GO) enrichment tests. The presence of a possible link between low Pi response, inositol polyphosphates, and JA signaling is therefore discussed.
[Mh] Termos MeSH primário: Brachypodium/metabolismo
Regulação da Expressão Gênica de Plantas
Fosfatos/deficiência
Raízes de Plantas/metabolismo
Transcrição Genética
[Mh] Termos MeSH secundário: Ciclopentanos/metabolismo
Oxilipinas/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclopentanes); 0 (Oxylipins); 0 (Phosphates); 6RI5N05OWW (jasmonic acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE



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