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[PMID]:26967539
[Au] Autor:Liu M; He R; Yang J; Zhao W; Zhou C
[Ad] Endereço:Department of Materials Science and Engineering, Jinan University , Guangzhou 510632, People's Republic of China.
[Ti] Título:Stripe-like Clay Nanotubes Patterns in Glass Capillary Tubes for Capture of Tumor Cells.
[So] Source:ACS Appl Mater Interfaces;8(12):7709-19, 2016 Mar.
[Is] ISSN:1944-8252
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Here, we used capillary tubes to evaporate an aqueous dispersion of halloysite nanotubes (HNTs) in a controlled manner to prepare a patterned surface with ordered alignment of the nanotubes . Sodium polystyrenesulfonate (PSS) was added to improve the surface charges of the tubes. An increased negative charge of HNTs is realized by PSS coating (from -26.1 mV to -52.2 mV). When the HNTs aqueous dispersion concentration is higher than 10%, liquid crystal phenomenon of the dispersion is found. A typical shear flow behavior and decreased viscosity upon shear is found when HNTs dispersions with concentrations higher than 10%. Upon drying the HNTs aqueous dispersion in capillary tubes, a regular pattern is formed in the wall of the tube. The width and spacing of the bands increase with HNTs dispersion concentration and decrease with the drying temperature for a given initial concentration. Morphology results show that an ordered alignment of HNTs is found especially for the sample of 10%. The patterned surface can be used as a model for preparing PDMS molding with regular micro-/nanostructure. Also, the HNTs rough surfaces can provide much higher tumor cell capture efficiency compared to blank glass surfaces. The HNTs ordered surfaces provide promising application for biomedical areas such as biosensors.
[Mh] Termos MeSH primário: Silicatos de Alumínio/química
Separação Celular/métodos
Nanotubos/química
Neoplasias/patologia
Polianetolsulfonato/química
[Mh] Termos MeSH secundário: Seres Humanos
Neoplasias/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Aluminum Silicates); 1302-87-0 (clay); 55963-78-5 (Polyanetholesulfonate)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160312
[St] Status:MEDLINE
[do] DOI:10.1021/acsami.6b01342


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[PMID]:26456688
[Au] Autor:Prax M; Vatani Shahmirzadi S; Götz F
[Ad] Endereço:Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine (IMIT), University of Tübingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
[Ti] Título:Sodium polyanethol sulfonate (SPS) falsifies protein staining and quantification and how to solve this problem.
[So] Source:J Microbiol Methods;118:176-81, 2015 Nov.
[Is] ISSN:1872-8359
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Sodium polyanethol sulfonate (SPS) is an anionic detergent with a broad range of activities and applications. While studying the excretion of cytoplasmic proteins in Staphylococcus aureus SPS was used as cell lysis inhibitor. When investigating the protein pattern of culture supernatants from cells grown in the absence or presence of SPS by Coomassie blue stained polyacrylamide gel the amount of protein bands was significantly decreased in the presence of SPS, suggesting that this effect was due to inhibition of cell lysis. However, various control studies showed that the apparent decreased protein secretion was an artifact due to the interference of SPS with Coomassie blue- and silver-staining. The only alternative method that was uninfluenced by SPS was imidazole-SDS-zinc staining. This is the method of choice particularly when protein interfering compounds are present in the extracts. For protein quantification in liquid samples the bicinchoninic acid (BCA) assay appeared to be the method of choice in the presence of SPS. The assay is based on neutral peptide bonds and is therefore rather insensitive to interfering compounds. This study shows that SPS and most likely also related detergents might falsify conventional protein staining and quantification methods.
[Mh] Termos MeSH primário: Erros de Diagnóstico
Polianetolsulfonato/metabolismo
Proteínas/análise
Coloração e Rotulagem/métodos
Staphylococcus aureus/química
[Mh] Termos MeSH secundário: Imidazóis/metabolismo
Quinolinas/metabolismo
Corantes de Rosanilina/metabolismo
Nitrato de Prata/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Imidazoles); 0 (Proteins); 0 (Quinolines); 0 (Rosaniline Dyes); 55963-78-5 (Polyanetholesulfonate); 78642-64-5 (Coomassie blue); 7GBN705NH1 (imidazole); 95IT3W8JZE (Silver Nitrate); CX56TX9Y1I (bicinchoninic acid)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151013
[St] Status:MEDLINE


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[PMID]:25960147
[Au] Autor:Johnson A; Brace C
[Ad] Endereço:Department of Biomedical Engineering and Department of Radiology, University of Wisconsin , Madison, Wisconsin , USA.
[Ti] Título:Heat transfer within hydrodissection fluids: An analysis of thermal conduction and convection using liquid and gel materials.
[So] Source:Int J Hyperthermia;31(5):551-9, 2015.
[Is] ISSN:1464-5157
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Interventional oncology procedures such as thermal ablation are becoming widely used for many tumours in the liver, kidney and lung. Thermal ablation refers to the focal destruction of tissue by generating cytotoxic temperatures in the treatment zone. Hydrodissection - separating tissues with fluids - protects healthy tissues adjacent to the ablation treatment zone to improve procedural safety, and facilitate more aggressive power application or applicator placement. However, fluids such as normal saline and 5% dextrose in water (D5W) can migrate into the peritoneum, reducing their protective efficacy. As an alternative, a thermo-gelable poloxamer 407 (P407) solution has been recently developed to facilitate hydrodissection procedures. We hypothesise that the P407 gel material does not provide convective heat dissipation from the ablation site, and therefore may alter the heat transfer dynamics compared to liquid materials during hydrodissection-assisted thermal ablation. The purpose of this study was to investigate the heat dissipation mechanics within D5W, liquid P407 and gel P407 hydrodissection barriers. Overall it was shown that the gel P407 dissipated heat primarily through conduction, whereas the liquid P407 and D5W dissipated heat through convection. Furthermore, the rate of temperature change within the gel P407 was greater than liquid P407 and D5W. Testing to evaluate the in vivo efficacy of the fluids with different modes of heat dissipation seems warranted for further study.
[Mh] Termos MeSH primário: Géis/química
Polianetolsulfonato/química
Condutividade Térmica
[Mh] Termos MeSH secundário: Convecção
Temperatura Alta
Hipertermia Induzida
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Gels); 55963-78-5 (Polyanetholesulfonate)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:150807
[Lr] Data última revisão:
150807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150512
[St] Status:MEDLINE
[do] DOI:10.3109/02656736.2015.1037799


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[PMID]:20711446
[Au] Autor:Villumsen S; Pedersen R; Krogfelt KA; Jensen JS
[Ad] Endereço:Department of Microbiological Surveillance and Research, Statens Serum Institut, Copenhagen, Denmark.
[Ti] Título:Expanding the diagnostic use of PCR in leptospirosis: improved method for DNA extraction from blood cultures.
[So] Source:PLoS One;5(8):e12095, 2010 Aug 11.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Leptospirosis is a neglected zoonosis of ubiquitous distribution. Symptoms are often non-specific and may range from flu-like symptoms to multi-organ failure. Diagnosis can only be made by specific diagnostic tests like serology and PCR. In non-endemic countries, leptospirosis is often not suspected before antibiotic treatment has been initiated and consequently, relevant samples for diagnostic PCR are difficult to obtain. Blood cultures are obtained from most hospitalized patients before antibiotic therapy and incubated for at least five days, thus providing an important source of blood for PCR diagnosis. However, blood cultures contain inhibitors of PCR that are not readily removed by most DNA-extraction methods, primarily sodium polyanetholesulfonate (SPS). METHODOLOGY/PRINCIPAL FINDINGS: In this study, two improved DNA extraction methods for use with blood cultures are presented and found to be superior in recovering DNA of Leptospira interrogans when compared with three previously described methods. The improved methods were easy and robust in use with all tested brands of blood culture media. Applied to 96 blood cultures obtained from 36 patients suspected of leptospirosis, all seven patients with positive convalescence serology were found positive by PCR if at least one anaerobic and one aerobic blood culture, sampled before antibiotic therapy were tested. CONCLUSIONS/SIGNIFICANCE: This study suggests that a specific and early diagnosis can be obtained in most cases of severe leptospirosis for up to five days after initiation of antimicrobial therapy, if PCR is applied to blood cultures already sampled as a routine procedure in most septic patients.
[Mh] Termos MeSH primário: Fracionamento Químico/métodos
Técnicas de Cultura
DNA Bacteriano/genética
DNA Bacteriano/isolamento & purificação
Leptospirose/sangue
Leptospirose/diagnóstico
Reação em Cadeia da Polimerase
[Mh] Termos MeSH secundário: DNA Bacteriano/sangue
Seres Humanos
Leptospira/efeitos dos fármacos
Leptospira/crescimento & desenvolvimento
Polianetolsulfonato/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Bacterial); 55963-78-5 (Polyanetholesulfonate)
[Em] Mês de entrada:1011
[Cu] Atualização por classe:141203
[Lr] Data última revisão:
141203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100817
[St] Status:MEDLINE


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[PMID]:20042630
[Au] Autor:Palarasah Y; Skjoedt MO; Vitved L; Andersen TE; Skjoedt K; Koch C
[Ad] Endereço:Research Unit of Immunology and Microbiology, Institute of Medical Biology, Faculty of Health Science, University of Southern Denmark, Odense, Denmark.
[Ti] Título:Sodium polyanethole sulfonate as an inhibitor of activation of complement function in blood culture systems.
[So] Source:J Clin Microbiol;48(3):908-14, 2010 Mar.
[Is] ISSN:1098-660X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors. We analyzed the effect of SPS on the three complement activation pathways: the classical, alternative, and lectin pathways, respectively. Inhibition of complement activity by SPS is caused by a blocking of complement activation and is not a result of complement consumption. The classical pathway is inhibited at SPS concentrations greater than 0.1 mg/ml, and complete inhibition is seen at 0.4 mg/ml. An SPS concentration of 0.5 mg/ml completely inhibits the binding of C1q and subsequent incorporation of C3, C4, and C9. The same was observed for the alternative pathway with an inhibition at SPS concentrations from 0.1 mg/ml and a complete inhibition from 0.4 mg/ml. Here, properdin binding was completely absent, and no incorporation of C3 and C9 was observed. In contrast, the lectin complement pathway remains unaffected at these SPS concentrations, and inhibition is first observed from 0.7 mg/ml. A complete inhibition required concentrations greater than 1 mg/ml. SPS is used in growth media (e.g., BACTEC and BacT/Alert) at concentrations from 0.3 to 0.5 mg/ml. The well-known finding that certain bacteria are growth inhibited by blood factors could therefore be a consequence of the lectin pathway, which is not inhibited at these concentrations. In addition, our findings also open up the possibility of a new assay for the assessment of the functional capacity of the lectin complement pathway.
[Mh] Termos MeSH primário: Técnicas Bacteriológicas/métodos
Sangue/imunologia
Ativação do Complemento/efeitos dos fármacos
Proteínas do Sistema Complemento/imunologia
Meios de Cultura/química
Fatores Imunológicos/farmacologia
Polianetolsulfonato/farmacologia
[Mh] Termos MeSH secundário: Bactérias/isolamento & purificação
Sangue/microbiologia
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Immunologic Factors); 55963-78-5 (Polyanetholesulfonate); 9007-36-7 (Complement System Proteins)
[Em] Mês de entrada:1005
[Cu] Atualização por classe:141204
[Lr] Data última revisão:
141204
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100101
[St] Status:MEDLINE
[do] DOI:10.1128/JCM.01985-09


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[PMID]:16145115
[Au] Autor:Varettas K; Mukerjee C; Taylor PC
[Ad] Endereço:Division of Microbiology, SEALS, St. George Hospital, Kogarah, New South Wales, Australia.
[Ti] Título:Anticoagulant carryover may influence clot formation in direct tube coagulase tests from blood cultures.
[So] Source:J Clin Microbiol;43(9):4613-5, 2005 Sep.
[Is] ISSN:0095-1137
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The tube coagulase test (TCT) performed directly from positive blood culture bottles has been used to reduce the turnaround time for identifying Staphylococcus aureus. Most reports have shown the test to be specific but often lacking sufficient sensitivity to be useful. In a prospective study of blood culture bottles (BCB) signaling positive, with a Gram-stained smear showing gram-positive cocci resembling staphylococci, the sensitivity of the direct TCT was improved by diluting the BCB broth 1:10 in saline before inoculating 0.1 ml into 1.0 ml of 10% pooled human plasma. It was hypothesized that the improved sensitivity might be explained by reduced carryover of the anticoagulant sodium polyanetholesulfonate (SPS) used in blood culture media. By titrating the inoculum size and the concentration of SPS in an in vitro checkerboard assay, it was shown that concentrations of SPS >0.0008% prevented plasma coagulation. The 1:10 dilution of blood culture broth reduced the amount of residual SPS carried over to the TCT to a level (0.0005%) that did not impair plasma coagulation. The direct TCT inoculated with a 1:10 saline dilution of blood culture broth achieved 100% specificity and sensitivity within 4 h of inoculation without reducing the quality or quantity of coagulum.
[Mh] Termos MeSH primário: Anticoagulantes
Sangue/microbiologia
Coagulase/metabolismo
Polianetolsulfonato
Kit de Reagentes para Diagnóstico
Staphylococcus aureus/isolamento & purificação
[Mh] Termos MeSH secundário: Anticoagulantes/química
Técnicas de Tipagem Bacteriana/instrumentação
Técnicas Bacteriológicas
Coagulação Sanguínea
Meios de Cultura
Seres Humanos
Polianetolsulfonato/química
Sensibilidade e Especificidade
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/classificação
Staphylococcus aureus/enzimologia
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anticoagulants); 0 (Coagulase); 0 (Culture Media); 0 (Reagent Kits, Diagnostic); 55963-78-5 (Polyanetholesulfonate)
[Em] Mês de entrada:0510
[Cu] Atualização por classe:140605
[Lr] Data última revisão:
140605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:050908
[St] Status:MEDLINE


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[PMID]:11791670
[Au] Autor:Ochiai T
[Ad] Endereço:Faculty of Pharmaceutical Science, Hokuriku University, Kanazawa, Ishikawa, Japan.
[Ti] Título:Staphylococcus aureus requires increased level of Ca(2+) or Mn(2+) to grow normally in a high-NaCl/low-Mg(2+) medium.
[So] Source:Microbiol Immunol;45(11):769-76, 2001.
[Is] ISSN:0385-5600
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Mg2+-availability in Staphylococcus aureus cells decreased significantly with increasing NaCl concentration in growth media. Cells grew in a NaCl-free, Chelex resin-treated complex medium only if the medium was supplemented with 50 microM MgCl2, while, growth was limited when the medium was further supplemented with 1.0 M NaCl. Cells grown in such a high-NaCl/low-Mg2+ medium exhibited the morphologic abnormality of larger than normal cells. Both sufficient growth and normal cell morphology were restored by increasing Mg2+ concentration in a high-NaCl medium, or by supplementation with either CaCl2 or MnSO4 in a high-NaCl/low-Mg2+ medium. Supplementing with BaCl2, SrCl2 or FeSO4, however, had no effect. These results indicate that Ca2+ and Mn2+ might play some essential role in the growth of Staphylococcus aureus in a high-NaCl/low-Mg2+ environment.
[Mh] Termos MeSH primário: Cálcio/farmacologia
Magnésio/farmacologia
Manganês/farmacologia
Cloreto de Sódio/farmacologia
Staphylococcus aureus/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Bário/metabolismo
Bário/farmacologia
Betaína/metabolismo
Cálcio/administração & dosagem
Cloreto de Cálcio/metabolismo
Meios de Cultura
Compostos Ferrosos/metabolismo
Magnésio/administração & dosagem
Manganês/administração & dosagem
Polianetolsulfonato/metabolismo
Cloreto de Sódio/administração & dosagem
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/metabolismo
Zinco/metabolismo
Zinco/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Ferrous Compounds); 24GP945V5T (Barium); 3SCV180C9W (Betaine); 42Z2K6ZL8P (Manganese); 451W47IQ8X (Sodium Chloride); 55963-78-5 (Polyanetholesulfonate); I38ZP9992A (Magnesium); J41CSQ7QDS (Zinc); M4I0D6VV5M (Calcium Chloride); SY7Q814VUP (Calcium)
[Em] Mês de entrada:0207
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:020117
[St] Status:MEDLINE


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[PMID]:11149167
[Au] Autor:Gamboa F; de la Rosa Z; Bustillo J; Mora MP; Hernández I; Mirque F
[Ad] Endereço:Centro de Investigaciones Odontológicas, Facultad de Odontología, Pontificia Universidad Javeriana, Carrera 7 No. 40-62, Bogotá, Colombia. gamboa@javercol.javeriana.edu.co
[Ti] Título:[Negative effect of the components of the lysis-centrifugation system in the growth of mycobacteria in MGIT and Septi-Chek AFB liquid media].
[Ti] Título:Efecto negativo de los componentes del sistema lisis-centrifugación en el crecimiento de micobacterias en los medios líquidos MGIT y Septi-Chek AFB..
[So] Source:Enferm Infecc Microbiol Clin;18(9):439-44, 2000 Nov.
[Is] ISSN:0213-005X
[Cp] País de publicação:Spain
[La] Idioma:spa
[Ab] Resumo:BACKGROUND: The lysis-centrifugation system (Isolator system) is a technique with excellent results in the recovery of mycobacteria from blood specimens. This system consists mainly of saponin (SAP), polypropylenglycol (PPG), and sodium polianthol sulfonate (SPS). The objective of this work was to determine the effect of SAP, PPG, and SPS on the growth of Mycobacterium avium, M. kansasii, M. tuberculosis, and M. xenopi in fluid culture media MGIT and Septi-Chek AFB. METHODS: Two concentrations each of SAP, PPG, and SPS were prepared, and were added in 0.1 ml amounts (alone, in pairs and in combination) to fluid media MGIT and Septi-Chek AFB. Fluid culture media were then in individually inoculated with two different concentrations (10(3) and 10(5) CFU/ml) of each of the four mycobacterial strains used in this study. Culture media were incubated at 37 degrees C and were checked for growth daily. RESULTS: SAP, PPG, and SPS did not inhibit growth of mycobacteria but growth of these strains was indeed retarded (a lengthier time was required for detection of bacterial growth compared with the positive control). Final concentrations of SAP, PPG, and SPS which retarded mycobacterial growth varied, depending upon species, mycobacterial inoculum size, and fluid culture media used. CONCLUSIONS: Components included in the lysy-centrifugation system (SAP, PPG, and SPS), either alone or in combination retarded growth of M. avium, M. kansasii, M. tuberculosis, and M. xenopi in 10(3) and 10(5) CFU/ml concentrations in fluid culture media MGIT and Septi-Chek AFB. These results suggest that strategies should be adopted to decrease the concentrations of these three components, present in the sediment of the processed blood by the Isolator System, which eventually are going to be added to fluid media MGIT and Septi-Check AFB.
[Mh] Termos MeSH primário: Técnicas Bacteriológicas/métodos
Mycobacterium/efeitos dos fármacos
Polianetolsulfonato/farmacologia
Propilenoglicol/farmacologia
Saponinas/farmacologia
[Mh] Termos MeSH secundário: Meios de Cultura/química
Meios de Cultura/farmacologia
Mycobacterium/crescimento & desenvolvimento
Mycobacterium avium/efeitos dos fármacos
Mycobacterium avium/crescimento & desenvolvimento
Mycobacterium kansasii/efeitos dos fármacos
Mycobacterium kansasii/crescimento & desenvolvimento
Mycobacterium xenopi/efeitos dos fármacos
Mycobacterium xenopi/crescimento & desenvolvimento
Micobactérias não Tuberculosas/crescimento & desenvolvimento
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Saponins); 55963-78-5 (Polyanetholesulfonate); 6DC9Q167V3 (Propylene Glycol)
[Em] Mês de entrada:0101
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:010110
[St] Status:MEDLINE


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[PMID]:9762525
[Au] Autor:Braga PC; Sala MT; Dal Sasso M; Pecile A; Annoni G; Vergani C
[Ad] Endereço:Department of Pharmacology, School of Medicine, University of Milan, Italy.
[Ti] Título:Age-associated differences in neutrophil oxidative burst (chemiluminescence).
[So] Source:Exp Gerontol;33(5):477-84, 1998 Aug.
[Is] ISSN:0531-5565
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phagocytic defensive functions consist of a sequence of events, including migration, phagocytosis, secretion, and the release of reactive oxygen species (ROS). The last of these (also called "oxidative burst") has not received due attention in the elderly, even though it can be considered the most important event in the process of killing an invading microorganism. The aim of the present study was to investigate the oxidative burst activity of polymorphonuclear neutrophil leukocytes (PMNs) in relation to age, using a technique that specifically identifies ROS production: luminol-amplified chemiluminescence (LACL). Besides the use of LACL, a particular feature of the study was the use of five rather than just one or two different stimulants: two particulate (Candida albicans and zymosan) and three soluble ones [N-formyl-methionyl-leucyl-phenylalanine (fMLP), phorbol 12 myristate 13 acetate (PMA), and polyanetholesulfonate (liquoid)]. This approach allowed us to observe a dichotomy between the effects of Candida and zymosan (particulates), which were not significantly different in the elderly subjects compared to the young controls, and those of fMLP, PMA, and liquoid (solubles), which showed a significant reduction in LACL in the elderly group. Considering the different results obtained with the various stimulants adopted that are all believed to have NADPH oxidase as a common final target of oxidative burst, it may be postulated that aging can influence the different transductional pathways in different ways.
[Mh] Termos MeSH primário: Neutrófilos/metabolismo
Espécies Reativas de Oxigênio/metabolismo
[Mh] Termos MeSH secundário: Adulto
Fatores Etários
Idoso
Idoso de 80 Anos ou mais
Candida albicans/fisiologia
Carcinógenos/farmacologia
Seres Humanos
Medições Luminescentes
Meia-Idade
N-Formilmetionina Leucil-Fenilalanina/farmacologia
Neutrófilos/química
Neutrófilos/efeitos dos fármacos
Polianetolsulfonato/farmacologia
Acetato de Tetradecanoilforbol/farmacologia
Zimosan/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carcinogens); 0 (Reactive Oxygen Species); 55963-78-5 (Polyanetholesulfonate); 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine); 9010-72-4 (Zymosan); NI40JAQ945 (Tetradecanoylphorbol Acetate)
[Em] Mês de entrada:9812
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:981008
[St] Status:MEDLINE


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[PMID]:9738025
[Au] Autor:Fredricks DN; Relman DA
[Ad] Endereço:Department of Medicine, Division of Infectious Diseases, Stanford University Medical Center, Stanford, California 94305, USA. fredrick@cmgm.stanford.edu
[Ti] Título:Improved amplification of microbial DNA from blood cultures by removal of the PCR inhibitor sodium polyanetholesulfonate.
[So] Source:J Clin Microbiol;36(10):2810-6, 1998 Oct.
[Is] ISSN:0095-1137
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Molecular methods are increasingly used to identify microbes in clinical samples. A common technical problem with PCR is failed amplification due to the presence of PCR inhibitors. Initial attempts at amplification of the bacterial 16S rRNA gene from inoculated blood culture media failed for this reason. The inhibitor persisted, despite numerous attempts to purify the DNA, and was identified as sodium polyanetholesulfonate (SPS), a common additive to blood culture media. Like DNA, SPS is a high-molecular-weight polyanion that is soluble in water but insoluble in alcohol. Accordingly, SPS tends to copurify with DNA. An extraction method was designed for purification of DNA from blood culture media and removal of SPS. Blood culture media containing human blood and spiked with Escherichia coli was subjected to an organic extraction procedure with benzyl alcohol, and removal of SPS was documented spectrophotometrically. Successful amplification of the extracted E. coli 16S rRNA gene was achieved by adding 5 microliter of undiluted processed sample DNA to a 50-microliter PCR mixture. When using other purification methods, the inhibitory effect of SPS could be overcome only by dilution of these samples. By our extraction technique, even uninoculated blood culture media were found to contain bacterial DNA when they were subjected to broad-range 16S rRNA gene consensus PCR. We conclude that the blood culture additive SPS is a potent inhibitor of PCR, is resistant to removal by traditional DNA purification methods, but can be removed by a benzyl alcohol extraction protocol that results in improved PCR performance.
[Mh] Termos MeSH primário: Bactérias/isolamento & purificação
Infecções Bacterianas/diagnóstico
DNA Bacteriano/sangue
Reação em Cadeia da Polimerase/métodos
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Bactérias/genética
Escherichia coli/genética
Seres Humanos
Indicadores e Reagentes
Dados de Sequência Molecular
Filogenia
Polianetolsulfonato
RNA Bacteriano/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Indicators and Reagents); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); 55963-78-5 (Polyanetholesulfonate)
[Em] Mês de entrada:9810
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:980917
[St] Status:MEDLINE



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