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[PMID]:27816005
[Au] Autor:Zhu Y; Qin Tan Y; Leung LK
[Ad] Endereço:Food and Nutritional Sciences Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
[Ti] Título:Assessing placental corticotrophin-releasing hormone disruption by hexestrol in a cell model.
[So] Source:Environ Toxicol Pharmacol;48:197-202, 2016 Dec.
[Is] ISSN:1872-7077
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Studies have shown that corticotrophin-releasing hormone (CRH) and relaxin are associated with early delivery. Our lab previously has shown the mycotoxin zeranol increases placental CRH expression. The mycotoxin is used in the farming industry to promote cattle growth, and some synthetic hormones are also used for the same purposes. In order to complete the picture of these growth promoting agents, we attempted to examine the synthetic hormones on the placental gene expression in the current study. Among the tested compounds, hexestrol induced the CRH mRNA and protein expression at 100nM in JEG-3 cells. As signal transduction pathways have been described in the transcriptional control previously, the activations of several protein kinases were determined. P38, PKCß and JNK were activated upon hexestrol treatment. Since the P38-inhibitor SB203580 prevented hexestrol from inducing CRH in a subsequent experiment, P38 was likely involved in the transcriptional regulation. Electrophoretic mobility shift assay revealed an increase in the CRE binding activity in CRH promoter. This study showed that hexestrol exposure might be a concern for pregnant women.
[Mh] Termos MeSH primário: Hormônio Liberador da Corticotropina/metabolismo
Estrogênios não Esteroides/toxicidade
Regulação da Expressão Gênica/efeitos dos fármacos
Hexestrol/toxicidade
Placenta/efeitos dos fármacos
[Mh] Termos MeSH secundário: Sítios de Ligação
Técnicas de Cultura de Células
Linhagem Celular
Hormônio Liberador da Corticotropina/genética
Proteínas de Ligação a DNA/metabolismo
Relação Dose-Resposta a Droga
Ensaio de Desvio de Mobilidade Eletroforética
Feminino
Seres Humanos
Placenta/citologia
Placenta/metabolismo
Gravidez
Regiões Promotoras Genéticas/genética
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA-Binding Proteins); 0 (Estrogens, Non-Steroidal); 0 (RNA, Messenger); 10BI795R7D (Hexestrol); 9015-71-8 (Corticotropin-Releasing Hormone)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170916
[Lr] Data última revisão:
170916
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


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[PMID]:27249165
[Au] Autor:Liu H; Qiao L; Gan N; Lin S; Cao Y; Hu F; Wang J; Chen Y
[Ad] Endereço:Faculty of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
[Ti] Título:Electro-deposited poly-luminol molecularly imprinted polymer coating on carboxyl graphene for stir bar sorptive extraction of estrogens in milk.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1027:50-6, 2016 Aug 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Electrochemical polymerization of luminol molecularly imprinted polymer on carboxyl graphene (MIP/CG) was developed as stir bar sorptive extraction (SBSE) coating for selective pre-concentration and specific recognition of bisphenol A (BPA), hexoestrol and diethylstilbestrol in milk samples. Luminol was employed as monomer and BPA as the template to prepare MIP under 0-0.6V electro-polymerization. Carboxyl graphene was modified on pencil lead as the substrate to increase extraction capacity. The preparation and extraction conditions affecting the extraction efficiency were optimized. Under the optimized conditions, a good linearity of three estrogens was obtained in the range of 4-1000ngmL(-1). The average recoveries at the three spiked levels of the three estrogens ranged from 83.4% to 96.3% with the relative standard deviations (RSD)≤7.1%. The limits of detection were in the range of 0.36-1.09ngmL(-1). The developed method with low cost, high selectivity and good reproducibility can be potentially applied for determining trace estrogens in complex food samples.
[Mh] Termos MeSH primário: Estrogênios não Esteroides/isolamento & purificação
Análise de Alimentos/métodos
Luminol/química
Leite/química
Impressão Molecular/métodos
Polímeros/química
[Mh] Termos MeSH secundário: Adsorção
Animais
Compostos Benzidrílicos/isolamento & purificação
Dietilestilbestrol/isolamento & purificação
Galvanoplastia/métodos
Grafite/química
Hexestrol/isolamento & purificação
Limite de Detecção
Fenóis/isolamento & purificação
Polimerização
Extração em Fase Sólida/métodos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzhydryl Compounds); 0 (Estrogens, Non-Steroidal); 0 (Phenols); 0 (Polymers); 10BI795R7D (Hexestrol); 5EXP385Q4F (Luminol); 731DCA35BT (Diethylstilbestrol); 7782-42-5 (Graphite); MLT3645I99 (bisphenol A)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170213
[Lr] Data última revisão:
170213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160602
[St] Status:MEDLINE


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[PMID]:26753984
[Au] Autor:Feng J; Sun M; Bu Y; Luo C
[Ad] Endereço:Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China.
[Ti] Título:Hollow fiber membrane-coated functionalized polymeric ionic liquid capsules for direct analysis of estrogens in milk samples.
[So] Source:Anal Bioanal Chem;408(6):1679-85, 2016 Feb.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Protein removal process is always time-consuming for the analysis of milk samples. In this work, hollow fiber membrane-coated functionalized polymeric ionic liquid (HF-PIL) capsules were synthesized and used as solid-phase microextraction (SPME) sorbent for direct analysis of estrogens in milk samples. The functionalized PIL monolith sorbent was obtained by copolymerization between 1-(3-aminopropyl)-3-(4-vinylbenzyl)imidazolium 4-styrenesulfonate IL monomer and 1,6-di(3-vinylimidazolium) hexane bishexafluorophosphate IL-crosslinking agent. A group of four capsules were installed as SPME device, to determine four kinds of estrogens (estrone, diethylstilbestrol, hexestrol, and 17α-ethynylestradiol) in milk samples, coupled to high performance liquid chromatography. Extraction and desorption conditions were optimized to get satisfactory extraction efficiency. Good linearity was obtained in the range of 5-200 µg L(-1). The limits of detection were 1 µg L(-1) for diethylstilbestrol and 2 µg L(-1) for 17α-ethynylestradiol, estrone, and hexestrol. The present method was applied to analyze the model analytes in different milk samples. Relative recoveries were in the range of 85.5-112%. The HF-PIL SPME capsules showed satisfactory extraction efficiency and high resistance to sample matrix interference.
[Mh] Termos MeSH primário: Estrogênios/análise
Análise de Alimentos/métodos
Líquidos Iônicos/química
Leite/química
Microextração em Fase Sólida/métodos
[Mh] Termos MeSH secundário: Animais
Cápsulas/química
Cromatografia Líquida de Alta Pressão/métodos
Desenho de Equipamento
Estrona/análise
Contaminação de Alimentos/análise
Hexestrol/análise
Limite de Detecção
Membranas Artificiais
Microextração em Fase Sólida/instrumentação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Capsules); 0 (Estrogens); 0 (Ionic Liquids); 0 (Membranes, Artificial); 10BI795R7D (Hexestrol); 2DI9HA706A (Estrone)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160113
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-015-9279-9


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[PMID]:26452846
[Au] Autor:George MJ; Marjanovic L; Williams DB
[Ad] Endereço:Department of Chemistry, University of Johannesburg, P.O. Box 524, Auckland Park 2006, South Africa; Department of Chemistry and Chemical Technology, National University of Lesotho, P.O. Roma 180, Lesotho.
[Ti] Título:Picogram-level quantification of some growth hormones in bovine urine using mixed-solvent bubble-in-drop single drop micro-extraction.
[So] Source:Talanta;144:445-50, 2015 Nov 01.
[Is] ISSN:1873-3573
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Growth hormones are important biologically active compounds. However, they can cause deleterious effects if not used with care and their use in farmed animals is banned in the European Union. This study presents the development and application of a mixed-solvent "bubble-in-drop single drop micro-extraction" (BID-SDME) method for enrichment of stilbene hormones in bovine urine samples. The hormones are quantified using GC-MS showing good linearity with the coefficient of determination (R(2)) of 0.999 and 0.999 for hexestrol and diethylstilbestrol, respectively, in the concentration range 0.05-10 ng mL(-1). Excellent precision (RSD<10%) and accuracy (using a bovine urine certified reference material) were obtained. The observed detection capability (CCα, or LOD) values were 0.01 ng mL(-1) (hexestrol), 0.03 ng mL(-1) (cis-diethylstilbestrol) and 0.02 ng mL(-1) (trans-diethylstilbestrol), respectively, while the decision limit (CCß, or LOQ) values were 0.03 ng mL(-1), 0.08 ng mL(-1) and 0.07 ng mL(-1), which are comparable to or better than those reported in literature. Importantly, sample handling is significantly simplified by our method and enrichment values are greatly enhanced. The results show that a 3:1 chloroform/toluene mixture gave the highest extraction efficiency with a drop-bubble ratio of 2:1. We highlight the importance of solvent density on the success of the BID-SDME method.
[Mh] Termos MeSH primário: Dietilestilbestrol/urina
Hexestrol/urina
[Mh] Termos MeSH secundário: Animais
Bovinos
Clorofórmio/química
Dietilestilbestrol/química
Cromatografia Gasosa-Espectrometria de Massas
Hexestrol/química
Hormônios/urina
Limite de Detecção
Solventes/química
Tolueno/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Hormones); 0 (Solvents); 10BI795R7D (Hexestrol); 3FPU23BG52 (Toluene); 731DCA35BT (Diethylstilbestrol); 7V31YC746X (Chloroform)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:151010
[Lr] Data última revisão:
151010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151011
[St] Status:MEDLINE


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[PMID]:25766811
[Au] Autor:Chen X; Ma Y; Chen D; Ma M; Li C
[Ad] Endereço:Laboratory of Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Materials Science, South-Central University for Nationalities, Wuhan 430074, China.
[Ti] Título:Electrochemical fabrication of polymerized imidazole-based ionic liquid bearing pyrrole moiety for sensitive determination of hexestrol in chicken meat.
[So] Source:Food Chem;180:142-9, 2015 Aug 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:1-[3-(tert-Butoxycarbonylamino)propyl]-3-[3-(N-pyrrole)propyl]imidazolium tetrafluoroborate [(t-Boc-APPPI)BF4], which is a novel pyrrolyl-functionalized ionic liquid, was synthesized and characterized. Subsequently, it was electrochemically deposited onto a glassy carbon electrode surface to fabricate a polymerized ionic liquid film electrode. X-ray photoelectron spectroscopy, scanning electron microscope and electrochemical impedance spectroscopy were used to confirm the successful polymerization of ionic liquid. Voltammetric behaviors of hexestrol at the film electrode were investigated. The oxidation peak slightly shifted towards positive potential, however, dramatically increased in peak current. Experimental conditions for hexestrol determination were optimized. The oxidation peak current is linear with hexestrol concentration in the range of 1.0 × 10(-8)-1.0 × 10(-5) mol L(-1). The detection limit is estimated to be 2.1 × 10(-9) mol L(-1) (S/N=3). Hexestrol in chicken meat was determined using the film electrode with good accuracy.
[Mh] Termos MeSH primário: Hexestrol/química
Imidazóis/química
Líquidos Iônicos/química
Pirróis/química
[Mh] Termos MeSH secundário: Animais
Galinhas
Polimerização
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Imidazoles); 0 (Ionic Liquids); 0 (Pyrroles); 10BI795R7D (Hexestrol); 7GBN705NH1 (imidazole)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150313
[Lr] Data última revisão:
150313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150314
[St] Status:MEDLINE


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[PMID]:25682006
[Au] Autor:Syasina IG; Shved NA
[Ad] Endereço:A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch of Russian Academy of Sciences, 17, Palchevskogo str., Vladivostok 690059, Russia. Electronic address: syasina@mail.ru.
[Ti] Título:Hexestrol- and nonylphenol-induced differential vitellogenin synthesis in female and male barfin plaice Liopsetta pinnifasciata.
[So] Source:Environ Toxicol Pharmacol;39(2):597-605, 2015 Mar.
[Is] ISSN:1872-7077
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The effects of hexestrol (HXS) and nonylphenol (NP) on plasma vitellogenin (Vtg) concentration in barfin plaice Liopsetta pinnifasciata was studied during spring and autumn experiment. In L. pinnifasciata two "complete" forms of Vtgs, namely VtgAa and VtgAb, were previously described which may be separated due to molecular mass of their largest polypeptide in SDS-PAGE. In spring, the injection of HXS led to an increase in Vtg concentrations in both females and males. SDS-PAGE analysis of plasma from HXS-exposed fish produced only one prominent band at a molecular mass of 180 kDa that corresponds to an increase in VtgAb levels. NP injected in fish in spring induced statistically significant increasing of Vtg concentration in males, and only one type of Vtg, as in case of HXS, accumulated in plasma. In autumn, the injection of HXS results to the increase of Vtg concentration in the plasma of females and males, electrophoretic analysis of plasma proteins showed that only a 98 kDa polypeptide, corresponding to the VtgAa-type showed a significant increase. The blood plasma ratios of VtgAa and VtgAb in experimental fish are discussed in relation to the season and stage of reproductive cycle.
[Mh] Termos MeSH primário: Estrogênios/toxicidade
Linguado/sangue
Hexestrol/toxicidade
Fenóis/toxicidade
Vitelogeninas/sangue
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Estrogens); 0 (Phenols); 0 (Vitellogenins); 10BI795R7D (Hexestrol); 79F6A2ILP5 (nonylphenol)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150216
[St] Status:MEDLINE


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[PMID]:24822457
[Au] Autor:Li L; Su M; Shi X; Wang Y; Wang M; He J
[Ti] Título:[Determination of estrogen residues in drinking water by on-line solid phase extraction based on sol-gel technique coupled with high performance liquid chromatography].
[So] Source:Se Pu;32(2):194-7, 2014 Feb.
[Is] ISSN:1000-8713
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:A method for the determination of diethylstilbestrol (DES), hexestrol (HEX) and dienestrol (DS) residues in drinking water was established by on-line solid phase extraction (SPE) coupled with high performance liquid chromatography (HPLC). The material synthesized on the base of sol-gel technology was employed as adsorbent. This material was prepared using 3-aminopropyltriethoxysilane (APTES) as the functional monomer, tetraethoxysilane (TEOS) as the crosslinking agent, and acetic acid as the initiator. The synthesized adsorbent showed outstanding property for the estrogen extraction. The estrogen can be caught effectively from water samples and the extraction can be achieved rapidly. Some important parameters, such as pH of sample solution, eluent solvents, loading flow rate, which might influence extraction efficiency, were optimized. The results indicated that the limit of detection (S/N = 3) of the developed method could reach 0.07-0.13 microg/L under the conditions of pH 7.0 of sample solution, methanol and 1% (v/v) acetic acid aqueous solution as the eluent solvent and the loading flow rate of 2 mL/min. The recoveries of the three estrogens from the water samples at three spiked levels ranged from 82.31% to 99.43% with RSD of 1.61%-7.15%. The method was simple, rapid, and suitable to detect the trace residues of estrogens in drinking water.
[Mh] Termos MeSH primário: Água Potável/análise
Estrogênios/análise
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Dienestrol
Dietilestilbestrol
Hexestrol
Polimetil Metacrilato
Propilaminas
Silanos
Extração em Fase Sólida
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drinking Water); 0 (Estrogens); 0 (Propylamines); 0 (Silanes); 0 (Water Pollutants, Chemical); 10BI795R7D (Hexestrol); 42064KRE49 (tetraethoxysilane); 731DCA35BT (Diethylstilbestrol); 9011-14-7 (Polymethyl Methacrylate); L8S6UBW552 (amino-propyl-triethoxysilane); RRW32X4U1F (Dienestrol)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140515
[St] Status:MEDLINE


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[PMID]:24636894
[Au] Autor:Hu WY; Kang XJ; Zhang C; Yang J; Ling R; Liu EH; Li P
[Ad] Endereço:State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, PR China; Nanjing Bureau of Quality and Technical Supervision, Nanjing, PR China.
[Ti] Título:Packed-fiber solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry for determination of diethylstilbestrol, hexestrol, and dienestrol residues in milk products.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;957:7-13, 2014 Apr 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A sensitive analytical method based on packed-fiber solid-phase extraction and high performance liquid chromatography-tandem mass spectrometry (PF SPE-HPLC-MS/MS) has been developed for determination of three synthetic stilbenes in milk. The stilbenes are extracted with acetonitrile, using sodium chloride, and purified with PF SPE using a cartridge containing electrospun polystyrene nanofibers. Parameters affecting the efficiency of PF SPE, such as pH and amount of salt, were optimized. Under optimal conditions, the limits of detection and quantification were 5-13pg/g and 15-37pg/g, respectively. Absolute recoveries varied between 60% and 85% at three different levels. The method was successfully applied for the determination of estrogenic stilbenes in a total of 69 milk samples. The method is sensitive and cost-effective in stilbene detection, and has potential in quality control of dairy products.
[Mh] Termos MeSH primário: Dienestrol/análise
Dietilestilbestrol/análise
Hexestrol/análise
Leite/química
Extração em Fase Sólida/métodos
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão/métodos
Dienestrol/química
Dienestrol/isolamento & purificação
Dietilestilbestrol/química
Dietilestilbestrol/isolamento & purificação
Hexestrol/química
Hexestrol/isolamento & purificação
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
Espectrometria de Massas em Tandem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
10BI795R7D (Hexestrol); 731DCA35BT (Diethylstilbestrol); RRW32X4U1F (Dienestrol)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:140401
[Lr] Data última revisão:
140401
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140319
[St] Status:MEDLINE


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[PMID]:23451515
[Au] Autor:Nong S; Lin F; Huang X; Yuan D
[Ad] Endereço:State Key Laboratory of Marine Environmental Science, College of the Environment and Ecology, Xiamen University, Xiamen 361005, China.
[Ti] Título:Preparation of a stir bar sorptive extraction coating based on molecularly imprinted polymer and its application in the extraction of dienestrol and hexestrol in complicated samples.
[So] Source:Se Pu;30(11):1133-42, 2012 Nov.
[Is] ISSN:1000-8713
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:A new stir bar sorptive extraction (SBSE) coating based on molecularly imprinted polymer (MIP) with diethylstilbestrol as replaced template molecule was prepared. The influences of the contents of template molecule and monomer in the polymerization mixture on the extraction performance of MIP-SBSE were investigated thoroughly. The MIP was characterized by elemental analysis, scanning electron microscopy and infrared spectroscopy. In order to evaluate the usability of the new coating, the MIP-SBSE was combined with high performance liquid chromatography (HPLC) and diode array detector (DAD) with dienestrol (DS) and hexestrol (HS) as detected solutes. To achieve optimally selective extraction performance for DS and HS, several parameters, including extraction and desorption times, desorption solvent, ionic strength and pH value in sample matrix were investigated. The results showed that under the optimized experimental conditions, the present method has high selectivity and sensitivity. When drying-redissolving procedure was taken during sample preparation, the limits of detection for DS and HS were as low as 0.04 microg/L and 0.14 micorg/L, respectively. Good linearities were obtained for analytes with the correlation coefficients (R2) above 0.99. Finally, the proposed method was successfully applied to the determination of DS and HS in wastewater, honey and cow urine samples. The recoveries of spiked target compounds in real samples ranged from 61.3% to 120%. The developed method is simple, selective, sensitive and applicable for the analysis of trace DS and HS in complicated samples.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Dienestrol/análise
Hexestrol/análise
Impressão Molecular
Extração em Fase Sólida/métodos
[Mh] Termos MeSH secundário: Adsorção
Fracionamento Químico/métodos
Dienestrol/isolamento & purificação
Hexestrol/isolamento & purificação
Polímeros/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Polymers); 10BI795R7D (Hexestrol); RRW32X4U1F (Dienestrol)
[Em] Mês de entrada:1404
[Cu] Atualização por classe:161018
[Lr] Data última revisão:
161018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130305
[St] Status:MEDLINE


  10 / 444 MEDLINE  
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[PMID]:22554413
[Au] Autor:Zhong Q; Hu Y; Hu Y; Li G
[Ad] Endereço:School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
[Ti] Título:Dynamic liquid-liquid-solid microextraction based on molecularly imprinted polymer filaments on-line coupling to high performance liquid chromatography for direct analysis of estrogens in complex samples.
[So] Source:J Chromatogr A;1241:13-20, 2012 Jun 08.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel sample preparation technique termed dynamic liquid-liquid-solid microextraction (DLLSME) was developed and on-line coupled to high performance liquid chromatography (HPLC) for direct extraction, desorption, and analysis of trace estrogens in complex samples. The DLLSME consists of the aqueous donor phase, the organic medium phase and the molecularly imprinted polymer filaments (MIPFs) as solid acceptor phase. The organic solvent with lesser density was directly added on top of the aqueous sample, and the dynamic extraction was performed by circulating the organic solvent through the MIPFs inserted into a PEEK tube which served as an extraction and desorption chamber. Afterwards, the extracted analytes on the MIPFs were on-line desorbed and then introduced into the HPLC for analysis. To evaluate the feasibility of the on-line system, a new DLLSME-HPLC method was developed for the analysis of five estrogens in aqueous samples by using 17ß-estradiol MIPFs as the solid phase. Under the optimized conditions, the enrichment factors of 51-70, limits of detection of 0.08-0.25 µg/L and precision within 4.5-6.9% were achieved. Furthermore, the proposed method was applied to the analysis of real samples including urine, milk and skin toner, satisfactory recovery (81.9-99.8%) and reproducibility (4.1-7.9%) were obtained. Especially, 0.59 µg/L of 17ß-estradiol was determined in female urine sample. The DLLSME offers an attractive alternative for direct analysis of trace analytes in aqueous samples and could potentially be extended to other adsorptive materials.
[Mh] Termos MeSH primário: Estrogênios/isolamento & purificação
Microextração em Fase Líquida/métodos
Impressão Molecular/instrumentação
Microextração em Fase Sólida/métodos
[Mh] Termos MeSH secundário: Adulto
Animais
Cromatografia Líquida de Alta Pressão/métodos
Cosméticos/química
Dietilestilbestrol/análise
Dietilestilbestrol/isolamento & purificação
Dietilestilbestrol/urina
Estradiol/análise
Estradiol/isolamento & purificação
Estradiol/urina
Estrogênios/análise
Estrogênios/urina
Estrona/análise
Estrona/isolamento & purificação
Estrona/urina
Etinilestradiol/análise
Etinilestradiol/isolamento & purificação
Etinilestradiol/urina
Feminino
Hexestrol/análise
Hexestrol/isolamento & purificação
Hexestrol/urina
Seres Humanos
Limite de Detecção
Leite/química
Polímeros
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cosmetics); 0 (Estrogens); 0 (Polymers); 10BI795R7D (Hexestrol); 2DI9HA706A (Estrone); 423D2T571U (Ethinyl Estradiol); 4TI98Z838E (Estradiol); 731DCA35BT (Diethylstilbestrol)
[Em] Mês de entrada:1207
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120505
[St] Status:MEDLINE
[do] DOI:10.1016/j.chroma.2012.04.017



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