Base de dados : MEDLINE
Pesquisa : D02.455.426.559.389.185.100 [Categoria DeCS]
Referências encontradas : 1310 [refinar]
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  1 / 1310 MEDLINE  
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[PMID]:28693991
[Au] Autor:Xue Z; Wang X; Rao H; Liu X; Lu X
[Ad] Endereço:Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China. Electronic address: xzh@nwnu.edu.cn.
[Ti] Título:A colorimetric sensor of cysteine based on self-assembly nanostructures of Fe -H O /Tetramethylbenzidine system with "On-Off" switching function.
[So] Source:Anal Biochem;534:1-9, 2017 Oct 01.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Many strategies have been explored for selectively and sensitively detecting cysteine in different samples. Here, a novel colorimetric sensor based on self-assembly nanostructures of Fe -H O /Tetramethylbenzidine system with dual-level logic gate function and colorimetric determination of cysteine were firstly explored. The proposed Fe -H O -TMB system provides a sensitive optical signal due to the selectively reductive ability of cysteine to the oxidized TMB and thus could be successfully applied to the construction of instant on-site visual detection method with a paper based test strip for cysteine determination in a sample solution as well as for a dual-level logic gate fabrication.
[Mh] Termos MeSH primário: Benzidinas/química
Técnicas Biossensoriais
Colorimetria
Cisteína/análise
Compostos Férricos/química
Peróxido de Hidrogênio/química
Nanoestruturas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzidines); 0 (Ferric Compounds); 3B3T5CB8EO (3,3',5,5'-tetramethylbenzidine); BBX060AN9V (Hydrogen Peroxide); K848JZ4886 (Cysteine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE


  2 / 1310 MEDLINE  
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[PMID]:28692846
[Au] Autor:Tallapragada SD; Layek K; Mukherjee R; Mistry KK; Ghosh M
[Ad] Endereço:National Institute of Technology, M. G. Avenue, Durgapur 713209, India.
[Ti] Título:Development of screen-printed electrode based immunosensor for the detection of HER2 antigen in human serum samples.
[So] Source:Bioelectrochemistry;118:25-30, 2017 Dec.
[Is] ISSN:1878-562X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, an immunosensor based on screen-printed electrode (SPE) has been developed for the detection of Human Epidermal Growth Factor Receptor-2 (HER2) antigen. The SPEs were fabricated and a sandwich enzyme linked immunosorbent assay (ELISA) format was followed for the construction of the immunosensor. The capture antibody (mouse anti-human ErbB2) was coated onto the electrode surface without any prior surface modification, followed by the addition of recombinant human HER2 antigen. Biotinylated goat anti-human ErbB2 was used as the detection antibody which was linked to streptavidin conjugated horseradish peroxidase (HRP). 3,3',5,5'-tetramethylbenzidine (TMB) was used as the substrate. The redox reaction was measured using cyclic voltammetry at scan rate of 50mV/s for the quantification of the antigen in solution. The biotin-avidin chemistry enabled the accurate detection of the antigen in nanogram levels. The amperometric signal obtained increased linearly with increase in the HER2 concentration and two-fold linear range was obtained between 5ng/ml-20ng/ml and 20-200ng/ml respectively. The limit of detection (LOD) and the limit of quantification (LOQ) of this immunosensor were found to be 4ng/ml and 5ng/ml respectively. The detection of HER2 in the serum samples of invasive and non-invasive breast cancer patients has been realized.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/instrumentação
Análise Química do Sangue/instrumentação
Ensaio de Imunoadsorção Enzimática/instrumentação
Impressão
Receptor ErbB-2/sangue
[Mh] Termos MeSH secundário: Anticorpos Imobilizados/química
Anticorpos Imobilizados/imunologia
Benzidinas/química
Eletroquímica
Eletrodos
Peroxidase do Rábano Silvestre/química
Peroxidase do Rábano Silvestre/metabolismo
Seres Humanos
Limite de Detecção
Receptor ErbB-2/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Immobilized); 0 (Benzidines); 3B3T5CB8EO (3,3',5,5'-tetramethylbenzidine); EC 1.11.1.- (Horseradish Peroxidase); EC 2.7.10.1 (ERBB2 protein, human); EC 2.7.10.1 (Receptor, ErbB-2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE


  3 / 1310 MEDLINE  
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[PMID]:28687493
[Au] Autor:El-Sayed R; Ye F; Asem H; Ashour R; Zheng W; Muhammed M; Hassan M
[Ad] Endereço:Division of Experimental Cancer Medicine (ECM), Department of Laboratory Medicine, Karolinska Institutet, SE-141 86 Stockholm, Sweden. Electronic address: ramy.el.sayed@ki.se.
[Ti] Título:Importance of the surface chemistry of nanoparticles on peroxidase-like activity.
[So] Source:Biochem Biophys Res Commun;491(1):15-18, 2017 Sep 09.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We report the studies on origin of peroxidase-like activity for gold nanoparticles, as well as the impact from morphology and surface charge of nanoparticles. For this purpose, we have synthesized hollow gold nanospheres (HAuNS) and gold nanorods (AuNR) with different morphology and surface chemistry to investigate their influence on the catalytic activity. We found that citrate-capped HAuNS show catalyzing efficiency in oxidation reaction of 3,3',5,5'-tetramethylbenzidine (TMB) by hydrogen peroxide (H O ) and it is superior to that of cetyltrimethylammonium bromide (CTAB)-capped AuNR. The kinetics of catalytic activities from HAuNS and AuNR were respectively studied under varied temperatures. The results indicated that surface chemistry rather than morphology of nanoparticles plays an important role in the catalytic reaction of substrate. Furthermore, influencing factors such as pH, amount of nanoparticle and H O concentration were also investigated on HAuNS-catalyzed system. The great impact of nanoparticle surface properties on catalytic reactions makes a paradigm in constructing nanozymes as peroxidase mimic for sensing application.
[Mh] Termos MeSH primário: Benzidinas/química
Ouro/química
Peróxido de Hidrogênio/química
Nanopartículas Metálicas/química
Peroxidase/química
[Mh] Termos MeSH secundário: Ativação Enzimática
Teste de Materiais
Nanopartículas Metálicas/ultraestrutura
Oxirredução
Tamanho da Partícula
Peroxidase/ultraestrutura
Especificidade por Substrato
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzidines); 3B3T5CB8EO (3,3',5,5'-tetramethylbenzidine); 7440-57-5 (Gold); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.7 (Peroxidase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170709
[St] Status:MEDLINE


  4 / 1310 MEDLINE  
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[PMID]:28654744
[Au] Autor:Luan Q; Gan N; Cao Y; Li T
[Ad] Endereço:State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science and Chemical Engineering, Ningbo University , Ningbo, 315211, PR China.
[Ti] Título:Mimicking an Enzyme-Based Colorimetric Aptasensor for Antibiotic Residue Detection in Milk Combining Magnetic Loop-DNA Probes and CHA-Assisted Target Recycling Amplification.
[So] Source:J Agric Food Chem;65(28):5731-5740, 2017 Jul 19.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A mimicking-enzyme-based colorimetric aptasensor was developed for the detection of kanamycin (KANA) in milk using magnetic loop-DNA-NMOF-Pt (m-L-DNA) probes and catalytic hairpin assembly (CHA)-assisted target recycling for signal amplification. The m-L-DNA probes were constructed via hybridization of hairpin DNA H1 (containing aptamer sequence) immobilized magnetic beads (m-H1) and signal DNA (sDNA, partial hybridization with H1) labeled nano Fe-MIL-88NH -Pt (NMOF-Pt-sDNA). In the presence of KANA and complementary hairpin DNA H2, the m-L-DNA probes decomposed and formed an m-H1/KANA intermediate, which triggered the CHA reaction to form a stable duplex strand (m-H1-H2) while releasing KANA again for recycling. Consequently, numerous NMOF-Pt-sDNA as mimicking enzymes can synergistically catalyze 3,3',5,5'-tetramethylbenzidine (TMB) for color development. The aptasensor exhibited high selectivity and sensitivity for KANA in milk with a detection limit of 0.2 pg mL within 30 min. The assay can be conveniently extended for on-site screening of other antibiotics in foods by simply changing the base sequence of the probes.
[Mh] Termos MeSH primário: Antibacterianos/análise
Técnicas Biossensoriais/métodos
Resíduos de Drogas/análise
Leite/química
[Mh] Termos MeSH secundário: Animais
Aptâmeros de Nucleotídeos/química
Benzidinas/química
Técnicas Biossensoriais/instrumentação
Bovinos
Colorimetria
DNA/química
Contaminação de Alimentos/análise
Ouro/química
Canamicina/análise
Limite de Detecção
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Aptamers, Nucleotide); 0 (Benzidines); 3B3T5CB8EO (3,3',5,5'-tetramethylbenzidine); 59-01-8 (Kanamycin); 7440-57-5 (Gold); 9007-49-2 (DNA)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02139


  5 / 1310 MEDLINE  
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[PMID]:28434989
[Au] Autor:Ying N; Sun T; Chen Z; Song G; Qi B; Bu S; Sun X; Wan J; Li Z
[Ad] Endereço:Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, China.
[Ti] Título:Colorimetric detection of microRNA based hybridization chain reaction for signal amplification and enzyme for visualization.
[So] Source:Anal Biochem;528:7-12, 2017 Jul 01.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:MicroRNAs (miRNAs) have key roles in gene expression and can be employed as biomarkers for early diagnosis of various diseases, especially cancers. Detection of miRNAs remains challenging and often requires detection platforms. Here, a horseradish peroxidase (HRP)-assisted hybridization chain reaction (HCR) for colorimetric detection of miR-155 was described. In the presence of target miRNA, the capture probe immobilized on the microplate sandwiched the target miR-155 with the 3' end of the reporter probe. Another exposed part of the RP at the 5'end triggered HCR producing double-stranded DNA polymers with multiple fluorescein isothiocyanates (FITC) for signal amplification. Finally, multiple HRP molecules were immobilized onto the long-range DNA nanostructures through FITC/anti-FITC monoclonal antibody interactions on the microplate for visualization by tetramethylbenzidine/H O system and the colorless substrate turned into the blue product. To obtain accurate data, the absorbance at 450 nm was calculated by microplate reader. The detection limit was 31.8 fM (3.18 amol). Furthermore, this biosensor showed high specificity and was able to discriminate sharply between target miRNA and mismatched sequences. And this approach could be easily applied to the detection of miR-155 in serum sample, thereby ascribing it for a wide application.
[Mh] Termos MeSH primário: Técnicas Biossensoriais
Colorimetria/métodos
MicroRNAs/análise
Hibridização de Ácido Nucleico
[Mh] Termos MeSH secundário: Benzidinas/química
Corantes Fluorescentes/química
Peroxidase do Rábano Silvestre/química
Seres Humanos
Peróxido de Hidrogênio/química
Limite de Detecção
MicroRNAs/sangue
MicroRNAs/genética
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzidines); 0 (Fluorescent Dyes); 0 (MIRN155 microRNA, human); 0 (MicroRNAs); 3B3T5CB8EO (3,3',5,5'-tetramethylbenzidine); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.- (Horseradish Peroxidase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170530
[Lr] Data última revisão:
170530
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170425
[St] Status:MEDLINE


  6 / 1310 MEDLINE  
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[PMID]:28283448
[Au] Autor:Wang A; Zhao H; Chen X; Tan B; Zhang Y; Quan X
[Ad] Endereço:Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, China), School of Environmental Science and Technology, Dalian University of Technology, Linggong Road 2, Dalian 116024, PR China.
[Ti] Título:A colorimetric aptasensor for sulfadimethoxine detection based on peroxidase-like activity of graphene/nickel@palladium hybrids.
[So] Source:Anal Biochem;525:92-99, 2017 May 15.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A sensitive, rapid and label-free colorimetric aptasensor for sulfadimethoxine (SDM) detection was developed based on the tunable peroxidase-like activity of graphene/nickel@palladium nanoparticle (Gr/Ni@Pd) hybrids. The addition of the SDM aptamer could inhibit the peroxidase-like catalytic activity of the hybrids. However, the target SDM and aptamer could be triggered tightly and recover the catalytic activity of the Gr/Ni@Pd hybrids. Due to the peroxidase-like catalytic activity, Gr/Ni@Pd could catalyze the decomposition of H O with releasing hydroxyl radicals which further oxidized reagent 3, 3', 5, 5'-Tetramethylbenzidine (TMB) to oxTMB accompanied with a colorless-to-blue color change. The original color change could be applied to obtain quantitative detection of SDM, due to the relationship between the concentration of the target and the color difference. As a result, this approach performed a linear response for SDM from 1 to 500 ng/mL with a limit detection of 0.7 ng/mL (S/N = 3) under the optimized conditions and realized the detection of SDM in spiked lake water samples. Therefore, this colorimetric aptasensor was an alternative assay for SDM detection in real water. Moreover, with its design principle, this work might be applied to detecting other small molecule by employing appropriate aptamer.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Colorimetria/métodos
Grafite/química
Níquel/química
Paládio/química
Peroxidase/metabolismo
Sulfadimetoxina/análise
[Mh] Termos MeSH secundário: Aptâmeros de Nucleotídeos
Benzidinas/química
Catálise
Peróxido de Hidrogênio/metabolismo
Lagos/química
Limite de Detecção
Nanopartículas/química
Oxirredução
Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aptamers, Nucleotide); 0 (Benzidines); 059QF0KO0R (Water); 30CPC5LDEX (Sulfadimethoxine); 3B3T5CB8EO (3,3',5,5'-tetramethylbenzidine); 5TWQ1V240M (Palladium); 7782-42-5 (Graphite); 7OV03QG267 (Nickel); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.7 (Peroxidase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170312
[St] Status:MEDLINE


  7 / 1310 MEDLINE  
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[PMID]:28259934
[Au] Autor:Liu Z; Liu J; Zhao L; Geng H; Ma J; Zhang Z; Yu D; Zhong C
[Ad] Endereço:Department of Urology, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China.
[Ti] Título:Curcumin reverses benzidine-induced epithelial-mesenchymal transition via suppression of ERK5/AP-1 in SV-40 immortalized human urothelial cells.
[So] Source:Int J Oncol;50(4):1321-1329, 2017 Apr.
[Is] ISSN:1791-2423
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Overexposure to benzidine has been manifested as an important cause of bladder cancer. However, the molecular mechanism of benzidine-induced malignancy is still insufficiently interpreted. Epithelial-mesenchymal transition (EMT) is a crucial pathophysiological process in embryonic development as well as initiation and development of epithelium-originated malignant tumors. The role of extracellular regulated protein kinase 5 (ERK5) in benzidine-meditated bladder cancer development has not been explored. In the present study, we explored the role of ERK5/AP-1 pathway in benzidine-induced EMT in human normal urothelial cells and the intervention effect of curcumin on bezidine-induced EMT. We found that benzidine-induced EMT in SV-40 immortalized human urothelial cells (SV-HUC-1) at low concentrations. We detected that ERK5/AP-1 pathway was notably activated. Specific ERK5 inhibitor, XMD8-92 was applied to determine the role of ERK5 in benzidine-induced EMT. Results indicated that XMD8-92 reversed the EMT process. Furthermore, curcumin effectively attenuated benzidine-induced urocystic EMT by suppressing ERK5/AP-1 pathway. In conclusion, the present study revealed the positive role of ERK5/AP-1 in benzidine-provoked urocystic EMT and the curcumin promising use in bladder cancer prevention and intervention via ERK5/AP-1 pathway.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Benzidinas/toxicidade
Curcumina/farmacologia
Transição Epitelial-Mesenquimal/efeitos dos fármacos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Proteína Quinase 7 Ativada por Mitógeno/metabolismo
Fator de Transcrição AP-1/metabolismo
Neoplasias da Bexiga Urinária/prevenção & controle
[Mh] Termos MeSH secundário: Benzodiazepinonas/farmacologia
Carcinogênese/efeitos dos fármacos
Células Cultivadas
Seres Humanos
Proteína Quinase 7 Ativada por Mitógeno/antagonistas & inibidores
Inibidores de Proteínas Quinases/farmacologia
Proteínas Quinases/metabolismo
Bexiga Urinária
Neoplasias da Bexiga Urinária/induzido quimicamente
Urotélio/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Benzidines); 0 (Benzodiazepinones); 0 (Protein Kinase Inhibitors); 0 (Transcription Factor AP-1); 0 (XMD 8-92); 2X02101HVF (benzidine); EC 2.7.- (Protein Kinases); EC 2.7.11.24 (MAPK7 protein, human); EC 2.7.11.24 (Mitogen-Activated Protein Kinase 7); IT942ZTH98 (Curcumin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170306
[St] Status:MEDLINE
[do] DOI:10.3892/ijo.2017.3887


  8 / 1310 MEDLINE  
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[PMID]:28189069
[Au] Autor:Sun XT; Zhang Y; Zheng DH; Yue S; Yang CG; Xu ZR
[Ad] Endereço:Research Center for Analytical Sciences, Northeastern University, Shenyang 110819, PR China.
[Ti] Título:Multitarget sensing of glucose and cholesterol based on Janus hydrogel microparticles.
[So] Source:Biosens Bioelectron;92:81-86, 2017 Jun 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A visualized sensing method for glucose and cholesterol was developed based on the hemispheres of the same Janus hydrogel microparticles. Single-phase and Janus hydrogel microparticles were both generated using a centrifugal microfluidic chip. For glucose sensing, concanavalin A and fluorescein labeled dextran used for competitive binding assay were encapsulated in alginate microparticles, and the fluorescence of the microparticles was positively correlated with glucose concentration. For cholesterol sensing, the microparticles embedded with γ-Fe O nanoparticles were used as catalyst for the oxidation of 3,3',5,5'-Tetramethylbenzidine by H O , an enzymatic hydrolysis product of cholesterol. And the color transition was more sensitive in the microparticles than in solutions, indicating the microparticles are more applicable for visualized determination. Furthermore, Janus microparticles were employed for multitarget sensing in the two hemespheres, and glucose and cholesterol were detected within the same microparticles without obvious interference. Besides, the particles could be manipulated by an external magnetic field. The glucose and cholesterol levels were measured in human serum utilizing the microparticles, which confirmed the potential application of the microparticles in real sample detection.
[Mh] Termos MeSH primário: Alginatos/química
Técnicas Biossensoriais/métodos
Glicemia/análise
Colesterol/sangue
Hidrogel de Polietilenoglicol-Dimetacrilato/química
Dispositivos Lab-On-A-Chip
Nanopartículas/química
[Mh] Termos MeSH secundário: Benzidinas/química
Técnicas Biossensoriais/instrumentação
Concanavalina A/química
Dextranos/química
Compostos Férricos/química
Corantes Fluorescentes/química
Ácido Glucurônico/química
Ácidos Hexurônicos/química
Seres Humanos
Peróxido de Hidrogênio/química
Limite de Detecção
Nanopartículas/ultraestrutura
Oxirredução
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alginates); 0 (Benzidines); 0 (Blood Glucose); 0 (Dextrans); 0 (Ferric Compounds); 0 (Fluorescent Dyes); 0 (Hexuronic Acids); 11028-71-0 (Concanavalin A); 1K09F3G675 (ferric oxide); 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate); 3B3T5CB8EO (3,3',5,5'-tetramethylbenzidine); 8A5D83Q4RW (Glucuronic Acid); 8C3Z4148WZ (alginic acid); 97C5T2UQ7J (Cholesterol); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170212
[St] Status:MEDLINE


  9 / 1310 MEDLINE  
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[PMID]:28167415
[Au] Autor:Xia Y; Liu M; Wang L; Yan A; He W; Chen M; Lan J; Xu J; Guan L; Chen J
[Ad] Endereço:Department of Pharmaceutical Analysis, The School of Pharmacy, Fujian Medical University, Fuzhou, Fujian Province 350108, PR China.
[Ti] Título:A visible and colorimetric aptasensor based on DNA-capped single-walled carbon nanotubes for detection of exosomes.
[So] Source:Biosens Bioelectron;92:8-15, 2017 Jun 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recently, many studies have shown the potential use of circulating exosomes as novel biomarkers for monitoring and predicting a number of complex diseases, including cancer. However, reliable and cost-effective detection of exosomes in routine clinical settings, still remain a difficult task, mainly due to the lack of adequately easy and fast assay platforms. Therefore, we demonstrate here the development of a visible and simple method for the detection of exosomes by integrating single-walled carbon nanotubes that being excellent water solubility (s-SWCNTs) and aptamer. Aptamers, specific to exosomes transmembrane protein CD63, are absorbed onto the surface of s-SWCNTs and improve the minic peroxidase activity of s-SWCNTs, which can efficiently catalyze H O -mediated oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) and lead to a change from colorless to blue in solution. However, after adding exosomes, the aptamers are bound with CD63, leaving from the surface of s-SWCNTs through conformational changes, which results the color of solution from deep to moderate, and this can be observed by the naked eye and monitored by UV-vis spectrometry. Under optimal conditions, the linear range of exosomes is estimated to be 1.84×10 to 2.21×10 particles/µL with a detection of limit (LOD) of 5.2×10 particles/µL. Consequently, a visible and simple approach detecting exosomes is successfully constructed. Moreover, this proposed colorimetric aptasensor can be universally applicable for the detection of other targets by simple change the aptamer.
[Mh] Termos MeSH primário: Aptâmeros de Nucleotídeos/química
Técnicas Biossensoriais/métodos
Colorimetria/métodos
Exossomos/química
Nanotubos de Carbono/química
Tetraspanina 30/análise
[Mh] Termos MeSH secundário: Benzidinas/química
Catálise
Compostos Cromogênicos/química
Seres Humanos
Peróxido de Hidrogênio/química
Células MCF-7
Nanotubos de Carbono/ultraestrutura
Oxirredução
Peroxidase/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aptamers, Nucleotide); 0 (Benzidines); 0 (CD63 protein, human); 0 (Chromogenic Compounds); 0 (Nanotubes, Carbon); 0 (Tetraspanin 30); 3B3T5CB8EO (3,3',5,5'-tetramethylbenzidine); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.7 (Peroxidase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE


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[PMID]:28135312
[Au] Autor:Hair PS; Sass LA; Krishna NK; Cunnion KM
[Ad] Endereço:Department of Pediatrics, Eastern Virginia Medical School, Norfolk, Virginia, United States of America.
[Ti] Título:Inhibition of Myeloperoxidase Activity in Cystic Fibrosis Sputum by Peptide Inhibitor of Complement C1 (PIC1).
[So] Source:PLoS One;12(1):e0170203, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Myeloperoxidase is the major peroxidase enzyme in neutrophil granules and implicated in contributing to inflammatory lung damage in cystic fibrosis. Free myeloperoxidase is present in cystic fibrosis lung fluid and generates hypochlorous acid. Here we report a new inhibitor of myeloperoxidase activity, Peptide Inhibitor of Complement C1 (PIC1). Using TMB as the oxidizing substrate, PIC1 inhibited myeloperoxidase activity in cystic fibrosis sputum soluble fractions by an average of a 3.4-fold decrease (P = 0.02). PIC1 also dose-dependently inhibited myeloperoxidase activity in a neutrophil lysate or purified myeloperoxidase by up to 28-fold (P < 0.001). PIC1 inhibited myeloperoxidase activity similarly, on a molar basis, as the specific myeloperoxidase inhibitor 4-Aminobenzoic acid hydrazide (ABAH) for various oxidizing substrates. PIC1 was able to protect the heme ring of myeloperoxidase from destruction by NaOCl, assayed by spectral analysis. PIC1 incubated with oxidized TMB reversed the oxidation state of TMB, as measured by absorbance at 450 nm, with a 20-fold reduction in oxidized TMB (P = 0.02). This result was consistent with an antioxidant mechanism for PIC1. In summary, PIC1 inhibits the peroxidase activity of myeloperoxidase in CF sputum likely via an antioxidant mechanism.
[Mh] Termos MeSH primário: Proteína Inibidora do Complemento C1/metabolismo
Fibrose Cística/enzimologia
Peroxidase/antagonistas & inibidores
Escarro/enzimologia
[Mh] Termos MeSH secundário: Compostos de Anilina/metabolismo
Antioxidantes/metabolismo
Benzidinas/metabolismo
Heme/metabolismo
Seres Humanos
Neutrófilos/metabolismo
Oxirredução
Peroxidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aniline Compounds); 0 (Antioxidants); 0 (Benzidines); 0 (Complement C1 Inhibitor Protein); 3B3T5CB8EO (3,3',5,5'-tetramethylbenzidine); 42VZT0U6YR (Heme); 5351-17-7 (4-aminobenzhydrazide); EC 1.11.1.7 (Peroxidase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0170203



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