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[PMID]:29029618
[Au] Autor:Bajgai J; Fadriquela A; Ara J; Begum R; Ahmed MF; Kim CS; Kim SK; Shim KY; Lee KJ
[Ad] Endereço:Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon, 26426, Republic of Korea.
[Ti] Título:Balneotherapeutic effects of high mineral spring water on the atopic dermatitis-like inflammation in hairless mice via immunomodulation and redox balance.
[So] Source:BMC Complement Altern Med;17(1):481, 2017 Oct 13.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Atopic dermatitis (AD) is a chronic relapsing allergic inflammatory skin disease that currently affects millions of children and adults worldwide. Drugs used to treat these inflammatory diseases include anti-histamines, corticosteroids and calcineurin inhibitors but these drugs have their limitations such as adverse effects with their long-term usage. Thus, researcher's interest in several alternative and complementary therapies are continually growing and balneotherapy is one of these approaches. Therefore, we investigate the bathing effect of high concentration mineral spring water (HMW) on redox balance and immune modulation in 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis like inflammation in hairless mice. METHODS: We induced AD-like inflammation by application of DNCB on the dorsal skin of female skh-1 hairless mice. The mice were treated with 100% pure HMW (PHMW) and 10% diluted HMW (DHMW) through bathing once a day for 4 weeks. Tacrolimus ointment (0.1%) was used as positive control (PC) and only DNCB treatment as negative control (NeC) group. The severity of skin lesion inflammation was assessed through clinical scoring and observing scratching behavior. Levels of immunoglobulin E (IgE) and inflammatory cytokines in serum were detected by ELISA and multiplex bead array system, and the levels of oxidative stress-related biomarkers and antioxidant enzyme were also measured. RESULTS: We found that HMW significantly decreased the scratching behavior in PHMW and DHMW groups at the 2nd week and in PHMW group at 4th week compared to NeC group. Likewise, serum IgE level was significantly decreased in DHMW group as compared to NeC group. In line, the level of inflammatory cytokines in serum such as interleukin (IL)-1ß, IL-13 and tumor necrosis factor-α were significantly inhibited in PHMW and DHMW groups compared to NeC group. In parallel, total reactive oxygen species (ROS) of serum level was significantly decreased in PHMW treatment groups compared to NeC group. Consistently, serum malondialdehyde (MDA) level in PHMW group was lower than in NeC group. By contrast, glutathione peroxidase (GPx) activity was significantly enhanced in PHMW than NeC. CONCLUSION: Collectively, our study indicates a balneotherapeutic effect of HMW on DNCB-induced AD like inflammation in hairless mice via immunomodulation and redox balance.
[Mh] Termos MeSH primário: Balneologia
Dermatite Atópica/terapia
Águas Minerais/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Dermatite Atópica/induzido quimicamente
Dermatite Atópica/patologia
Dinitroclorobenzeno/efeitos adversos
Modelos Animais de Doenças
Feminino
Imunomodulação
Camundongos
Camundongos Pelados
Oxirredução
Pele/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mineral Waters); GE3IBT7BMN (Dinitrochlorobenzene)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171015
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1985-8


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[PMID]:28889842
[Au] Autor:Chen C; Liu X; Li Y; Liang H; Li K; Li J; Cheng C; Liu X; Zhong S; Li L; Wang Y
[Ad] Endereço:Guangzhou Youdi Biotechnology Company, Guangzhou, Guangdong, China.
[Ti] Título:Effects of Acupuncture on 1-chloro-2,4-dinitrochlorobenzene-induced Allergic Contact Dermatitis in Mice.
[So] Source:J Acupunct Meridian Stud;10(4):252-260, 2017 Aug.
[Is] ISSN:2093-8152
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Allergic contact dermatitis (ACD) is a chronic inflammatory skin disease. Topical corticosteroids are the first-line therapy for ACD despite their significant adverse effects. Acupuncture has been widely used in the treatment of various skin diseases, but its underlying mechanism remains unrevealed. In this study, we investigated the characteristics of acupuncture treatment based on effectiveness and mechanism. BALB/c mice received 1-chloro-2,4-dinitrobenzene (DNCB) application to build AD-like model. Results showed that acupuncture was an effective treatment method in inhibiting inflammatory conditions, serum IgE levels, and expression of proinflammatory cytokine Th2 (IL-4, IL-6), and Th2 (IL-1ß, TNF-α) mRNA compared with DNCB treatment. Acupuncture treatment also inhibited nuclear factor-κB p65, phosphorylation of IκBα, and phosphorylation of occludin proteins expression. Furthermore, it could improve the expression of epidermal growth factor in both mRNA and protein levels. These results suggest that acupuncture, as an alternative therapy treatment for its no significant side effects, was effective in alleviating ACD by reducing proinflammatory cytokines and changing proteins' expression.
[Mh] Termos MeSH primário: Terapia por Acupuntura
Dermatite Alérgica de Contato/terapia
[Mh] Termos MeSH secundário: Animais
Citocinas/análise
Citocinas/metabolismo
Dermatite Alérgica de Contato/imunologia
Dermatite Alérgica de Contato/patologia
Dermatite Alérgica de Contato/fisiopatologia
Dinitroclorobenzeno/efeitos adversos
Modelos Animais de Doenças
Fator de Crescimento Epidérmico/análise
Fator de Crescimento Epidérmico/metabolismo
Feminino
Imunoglobulina E/análise
Camundongos
Camundongos Endogâmicos BALB C
Ocludina/análise
Ocludina/metabolismo
Pele/química
Pele/efeitos dos fármacos
Pele/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Occludin); 37341-29-0 (Immunoglobulin E); 62229-50-9 (Epidermal Growth Factor); GE3IBT7BMN (Dinitrochlorobenzene)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE


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[PMID]:28882453
[Au] Autor:Tharuka MDN; Bathige SDNK; Lee J
[Ad] Endereço:Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province 63243, Republic of Korea.
[Ti] Título:Molecular cloning, biochemical characterization, and expression analysis of two glutathione S-transferase paralogs from the big-belly seahorse (Hippocampus abdominalis).
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;214:1-11, 2017 Dec.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Glutathione S-transferases (GSTs, EC 2.5.1.18) are important Phase II detoxifying enzymes that catalyze hydrophobic, electrophilic xenobiotic substance with the conjugation of reduced glutathione (GSH). In this study, GSTµ and GSTρ paralogs of GST in the big belly seahorse (Hippocampus abdominalis; HaGSTρ, HaGSTµ) were biochemically, molecularly, functionally characterized to determine their detoxification range and protective capacities upon different pathogenic stresses. HaGSTρ and HaGSTµ are composed of coding sequences of 681bp and 654bp, which encode proteins 225 and 217 amino acids, with predicted molecular masses of 26.06kDa and 25.74kDa respectively. Sequence analysis revealed that both HaGSTs comprise the characteristic GSH-binding site in the thioredoxin-like N-terminal domain and substrate binding site in the C-terminal domain. The recombinant HaGSTρ and HaGSTµ proteins catalyzed the model GST substrate 1-chloro-2, 4-dinitrobenzene (CDNB). Enzyme kinetic analysis revealed different K and V values for each rHaGST, suggesting that they have different conjugation rates. The optimum conditions (pH, temperature) and inhibitory assays of each protein demonstrated different optimal ranges. However, HaGSTµ was highly expressed in the ovary and gill, whereas HaGSTρ was highly expressed in the gill and pouch. mRNA expression of HaGSTρ and HaGSTµ was significantly elevated upon lipopolysaccharide, Poly (I:C), and Edwardsiella tarda challenges in liver and in blood cells as well as with Streptococcus iniae challenge in blood cells. From these collective experimental results, we propose that HaGSTρ and HaGSTµ are effective in detoxifying xenobiotic toxic agents, and importantly, their mRNA expression could be stimulated by immunological stress signals in the aquatic environment.
[Mh] Termos MeSH primário: Proteínas de Peixes/química
Glutationa Transferase/química
Smegmamorpha/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sítios de Ligação
Clonagem Molecular
Dinitroclorobenzeno/imunologia
Dinitroclorobenzeno/metabolismo
Escherichia coli/genética
Escherichia coli/metabolismo
Proteínas de Peixes/agonistas
Proteínas de Peixes/genética
Proteínas de Peixes/imunologia
Expressão Gênica
Glutationa Transferase/genética
Glutationa Transferase/imunologia
Isoenzimas/química
Isoenzimas/genética
Isoenzimas/imunologia
Cinética
Lipopolissacarídeos/farmacologia
Modelos Moleculares
Especificidade de Órgãos
Filogenia
Poli I-C/farmacologia
Ligação Proteica
Domínios e Motivos de Interação entre Proteínas
Estrutura Secundária de Proteína
Estrutura Terciária de Proteína
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Smegmamorpha/classificação
Smegmamorpha/imunologia
Smegmamorpha/microbiologia
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins); 0 (Isoenzymes); 0 (Lipopolysaccharides); 0 (Recombinant Proteins); EC 2.5.1.18 (Glutathione Transferase); GE3IBT7BMN (Dinitrochlorobenzene); O84C90HH2L (Poly I-C)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE


  4 / 2251 MEDLINE  
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[PMID]:28842127
[Au] Autor:Arbildi P; Turell L; López V; Alvarez B; Fernández V
[Ad] Endereço:Cátedra de Inmunología/DEPBIO, Facultad de Química, Universidad de la República, Montevideo, 11600, Uruguay.
[Ti] Título:Mechanistic insights into EgGST1, a Mu class glutathione S-transferase from the cestode parasite Echinococcus granulosus.
[So] Source:Arch Biochem Biophys;633:15-22, 2017 Nov 01.
[Is] ISSN:1096-0384
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glutathione transferases (GSTs) comprise a major detoxification system in helminth parasites, displaying both catalytic and non-catalytic activities. The kinetic mechanism of these enzymes is complex and depends on the isoenzyme which is being analyzed. Here, we characterized the kinetic mechanism of rEgGST1, a recombinant form of a cytosolic GST from Echinococcus granulosus (EgGST1), which is related to the Mu-class of mammalian enzymes, using the canonical substrates glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB). Initial rate and product inhibition studies were consistent with a steady-state random sequential mechanism, where both substrates are bound to the enzyme before the products are released. Kinetic constants were also determined (pH 6.5 and 30 °C). Moreover, rEgGST1 lowered the pK of GSH from 8.71 ± 0.07 to 6.77 ± 0.08, and enzyme-bound GSH reacted with CDNB 1 × 10 times faster than free GSH at pH 7.4. Finally, the dissociation of the enzyme-GSH complex was studied by means of intrinsic fluorescence, as well as that of the complex with the anthelminth drug mebendazole. This is the first report on mechanistic issues related to a helminth parasitic GST.
[Mh] Termos MeSH primário: Echinococcus granulosus/química
Glutationa Transferase/metabolismo
Glutationa/metabolismo
Proteínas de Helminto/metabolismo
Proteínas Recombinantes de Fusão/metabolismo
[Mh] Termos MeSH secundário: Animais
Anti-Helmínticos/farmacologia
Clonagem Molecular
Dinitroclorobenzeno/metabolismo
Echinococcus granulosus/enzimologia
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Glutationa Transferase/antagonistas & inibidores
Glutationa Transferase/genética
Proteínas de Helminto/antagonistas & inibidores
Proteínas de Helminto/genética
Concentração de Íons de Hidrogênio
Inativação Metabólica/genética
Isoenzimas/genética
Isoenzimas/metabolismo
Cinética
Mebendazol/farmacologia
Ligação Proteica
Proteínas Recombinantes de Fusão/genética
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Helminth Proteins); 0 (Isoenzymes); 0 (Recombinant Fusion Proteins); 81G6I5V05I (Mebendazole); EC 2.5.1.18 (Glutathione Transferase); GAN16C9B8O (Glutathione); GE3IBT7BMN (Dinitrochlorobenzene)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170827
[St] Status:MEDLINE


  5 / 2251 MEDLINE  
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[PMID]:28826779
[Au] Autor:Tsuchiyama H; Maeda A; Nakajima M; Kitsukawa M; Takahashi K; Miyoshi T; Mutsuga M; Asaoka Y; Miyamoto Y; Oshida K
[Ad] Endereço:Pharmaceutical Research Laboratories, Toray Industries Inc., 10-1, Tebiro 6-chome, Kamakura, Kanagawa, 248-8555, Japan.
[Ti] Título:Gene expression profiles in auricle skin as a possible additional endpoint for determination of sensitizers: A multi-endpoint evaluation of the local lymph node assay.
[So] Source:Toxicol Lett;280:133-141, 2017 Oct 05.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The murine local lymph node assay (LLNA) is widely used to test chemicals to induce skin sensitization. Exposure of mouse auricle skin to a sensitizer results in proliferation of local lymph node T cells, which has been measured by in vivo incorporation of H -methyl thymidine or 5-bromo-2'-deoxyuridine (BrdU). The stimulation index (SI), the ratio of the mean proliferation in each treated group to that in the concurrent vehicle control group, is frequently used as a regulatory-authorized endpoint for LLNA. However, some non-sensitizing irritants, such as sodium dodecyl sulfate (SDS) or methyl salicylate (MS), have been reported as false-positives by this endpoint. In search of a potential endpoint to enhance the specificity of existing endpoints, we evaluated 3 contact sensitizers; (hexyl cinnamic aldehyde [HCA], oxazolone [OXA], and 2,4-dinitrochlorobenzene [DNCB]), 1 respiratory sensitizer (toluene 2,4-diisocyanate [TDI]), and 2 non-sensitizing irritants (MS and SDS) by several endpoints in LLNA. Each test substance was applied to both ears of female CBA/Ca mice daily for 3 consecutive days. The ears and auricle lymph node cells were analyzed on day 5 for endpoints including the SI value, lymph node cell count, cytokine release from lymph node cells, and histopathological changes and gene expression profiles in auricle skin. The SI values indicated that all the test substances induced significant proliferation of lymph node cells. The lymph node cell counts showed no significant changes by the non-sensitizers assessed. The inflammatory findings of histopathology were similar among the auricle skins treated by sensitizers and irritants. Gene expression profiles of cytokines IFN-γ, IL-4, and IL-17 in auricle skin were similar to the cytokine release profiles in draining lymph node cells. In addition, the gene expression of the chemokine CXCL1 and/or CXCL2 showed that it has the potential to discriminate sensitizers and non-sensitizing irritants. Our results suggest that multi-endpoint analysis in the LLNA leads to a better determination of the sensitizing potential of test substances. We also show that the gene expression of CXCL1 and/or CXCL2, which is involved in elicitation of contact hypersensitivity (CHS), can be a possible additional endpoint for discrimination of sensitizing compounds in LLNA.
[Mh] Termos MeSH primário: Pavilhão Auricular/metabolismo
Ensaio Local de Linfonodo
Pele/metabolismo
Transcriptoma/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Citocinas/genética
Citocinas/metabolismo
Dinitroclorobenzeno/toxicidade
Feminino
Regulação da Expressão Gênica/efeitos dos fármacos
Camundongos
Camundongos Endogâmicos CBA
Oxazolona/toxicidade
Salicilatos/toxicidade
Dodecilsulfato de Sódio/toxicidade
Tolueno 2,4-Di-Isocianato/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Salicylates); 15646-46-5 (Oxazolone); 17X7AFZ1GH (Toluene 2,4-Diisocyanate); 368GB5141J (Sodium Dodecyl Sulfate); 78243HXH5O (2,6-diisocyanatotoluene); GE3IBT7BMN (Dinitrochlorobenzene); LAV5U5022Y (methyl salicylate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE


  6 / 2251 MEDLINE  
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[PMID]:28655324
[Au] Autor:Choi JH; Song YS; Lee HJ; Kim GC; Hong JW
[Ad] Endereço:Department of Internal Medicine, School of Korean Medicine, Pusan National University, Yangsan, 626-870, South Korea.
[Ti] Título:The topical application of low-temperature argon plasma enhances the anti-inflammatory effect of Jaun-ointment on DNCB-induced NC/Nga mice.
[So] Source:BMC Complement Altern Med;17(1):340, 2017 Jun 27.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Jaun-ointment (JO), also known as Shiunko in Japan, is one of the most popular medicinal formulae used in Korean traditional medicine for the external treatment of skin wound and inflammatory skin conditions. Since JO is composed of crude mixture of two herbal extracts (radix of Lithospermum erythrorhizon Siebold & Zucc and Angelica gigas Nakai), those been proved its anti-inflammatory activities in-vitro and in-vivo, JO has been expected as a good alternative treatment option for atopic dermatitis (AD). However, due to the lack of strategies for the penetrating methods of JO's various anti-inflammatory elements into the skin, an effective and safe transdermal drug delivery system needs to be determined. Here, low-temperature argon plasma (LTAP) was adopted as an ancillary partner of topically applied JO in a mice model of AD and the effectiveness was examined. METHODS: Dorsal skins of NC/Nga mice were challenged with DNCB (2,4-dinitrochlorobenzene) to induce AD. AD-like skin lesions were treated with JO alone, or in combination with LTAP. Inflammatory activity in the skin tissues was evaluated by histological analysis and several molecular biological tests. RESULTS: LTAP enhanced the effect of JO on AD-like skin lesion. Topical application of JO partially inhibited the development of DNCB-induced AD, shown by the moderate reduction of eosinophil homing and pro-inflammatory cytokine level. Combined treatment of JO and LTAP dramatically inhibited AD phenotypes. Interestingly, treatment with JO alone did not affect the activity of nuclear factor (NF)κB/RelA in the skin, but combined treatment of LTAP-JO blocked DCNB-mediated NFκB/RelA activation. CONCLUSIONS: LTAP markedly enhanced the anti-inflammatory activity of JO on AD-like skin lesions. The effect of LTAP may be attributed to enhancement of drug penetration and regulation of NFκB activity. Therefore, the combination treatment of JO and LTAP could be a potential strategy for the treatment of AD.
[Mh] Termos MeSH primário: Anti-Inflamatórios/administração & dosagem
Argônio/administração & dosagem
Dermatite Atópica/tratamento farmacológico
Medicamentos de Ervas Chinesas/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Dermatite Atópica/etiologia
Dermatite Atópica/genética
Dermatite Atópica/imunologia
Dinitroclorobenzeno/efeitos adversos
Modelos Animais de Doenças
Feminino
Seres Humanos
Japão
Masculino
Camundongos
NF-kappa B/genética
NF-kappa B/imunologia
Pomadas/administração & dosagem
Gases em Plasma/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Drugs, Chinese Herbal); 0 (NF-kappa B); 0 (Ointments); 0 (Plasma Gases); 0 (shiunko); 67XQY1V3KH (Argon); GE3IBT7BMN (Dinitrochlorobenzene)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1850-9


  7 / 2251 MEDLINE  
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[PMID]:28440548
[Au] Autor:Betts RJ; Perkovic A; Mahapatra S; Del Bufalo A; Camara K; Howell AR; Martinozzi Teissier S; De Libero G; Mori L
[Ad] Endereço:Singapore Immunology Network, Agency for Science, Technology and Research, Singapore.
[Ti] Título:Contact sensitizers trigger human CD1-autoreactive T-cell responses.
[So] Source:Eur J Immunol;47(7):1171-1180, 2017 Jul.
[Is] ISSN:1521-4141
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Allergic contact dermatitis is a primarily T-cell-mediated inflammatory skin disease induced by exposure to small molecular-weight haptens, which covalently bind to proteins. The abundance of cutaneous T cells that recognize CD1a antigen-presenting molecules raises the possibility that MHC-independent antigen presentation may be relevant in some hapten-driven immune responses. Here we examine the ability of contact sensitizers to influence CD1-restricted immunity. Exposure of human antigen-presenting cells such as monocyte-derived dendritic cells and THP-1 cells to the prototypical contact sensitizer dinitrochlorobenzene potentiated the response of CD1a- and CD1d-autoreactive T cells, which released a vast array of cytokines in a CD1- and TCR-dependent manner. The potentiating effects of dinitrochlorobenzene depended upon newly synthesized CD1 molecules and the presence of endogenous stimulatory lipids. Further examination of a broad panel of contact sensitizers revealed 1,4-benzoquinone, resorcinol, isoeugenol, and cinnamaldehyde to activate the same type of CD1-restricted responses. These findings provide a basis for the antigen-specific activation of skin-associated CD1-restricted T cells by small molecules and may have implications for contact sensitizer-induced inflammatory skin diseases.
[Mh] Termos MeSH primário: Antígenos CD1/imunologia
Dermatite de Contato/imunologia
Células T Matadoras Naturais/imunologia
Linfócitos T/imunologia
[Mh] Termos MeSH secundário: Acroleína/análogos & derivados
Acroleína/farmacologia
Apresentação do Antígeno
Benzoquinonas/farmacologia
Linhagem Celular
Células Dendríticas/imunologia
Dinitroclorobenzeno/farmacologia
Eugenol/análogos & derivados
Eugenol/farmacologia
Seres Humanos
Lipídeos/imunologia
Ativação Linfocitária
Monócitos/efeitos dos fármacos
Resorcinóis/farmacologia
Pele/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD1); 0 (Benzoquinones); 0 (Lipids); 0 (Resorcinols); 3T8H1794QW (Eugenol); 5M0MWY797U (isoeugenol); 7864XYD3JJ (Acrolein); GE3IBT7BMN (Dinitrochlorobenzene); SR60A3XG0F (cinnamic aldehyde); YUL4LO94HK (resorcinol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.1002/eji.201746939


  8 / 2251 MEDLINE  
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[PMID]:28418286
[Au] Autor:Jiang X; Lan Y; Wei B; Dai C; Gu Y; Ma J; Liu X; Umezawa K; Zhang Y
[Ad] Endereço:a Department of Pharmacology, School of Life Science and Biopharmaceutics , Shenyang Pharmaceutical University , Shenyang , China.
[Ti] Título:External application of NF-κB inhibitor DHMEQ suppresses development of atopic dermatitis-like lesions induced with DNCB/OX in BALB/c mice.
[So] Source:Immunopharmacol Immunotoxicol;39(3):157-164, 2017 Jun.
[Is] ISSN:1532-2513
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Dehydroxymethylepoxyquinomicin (DHMEQ) which is originally developed as an analog of antibiotic epoxyquinomicin C is a specific and potent inhibitor of NF-κB and has been shown to possess promising potential as an anti-inflammatory and anti-tumor agent. OBJECTIVE: This study examines DHMEQ's effect on therapeutic potential for atopic dermatitis (AD)-like lesions. MATERIALS AND METHODS: AD lesions were chronically induced by the repetitive and alternative application of 2,4-dinitrochlorobenzene (DNCB) and oxazolone (OX) on ears in BALB/c mice. The mice were then externally treated with DHMEQ ointment. Macroscopic and microscopic changes of the skin lesions were observed and recorded. RESULTS: DHMEQ inhibited ear swelling and relieved clinical symptoms of the AD-like lesions induced by DNCB/OX in BALB/c mice. Histopathology examination illustrated that it significantly decreased DNCB/OX-induced epidermal thickness, the infiltration of inflammatory cells, and the count of mast cell. The elevated level of immunoglobulin E (IgE) in serum and the mRNA levels of interferon γ (IFN-γ), interleukin 4 (IL-4) and IL-13 in the ear tissues, were also suppressed by DHMEQ. DISCUSSION AND CONCLUSION: This study indicated that DHMEQ would be useful for the treatment of AD.
[Mh] Termos MeSH primário: Benzamidas/farmacologia
Cicloexanonas/farmacologia
Dermatite Atópica
Dinitroclorobenzeno/toxicidade
Epiderme
NF-kappa B/antagonistas & inibidores
Oxazolona/toxicidade
[Mh] Termos MeSH secundário: Animais
Citocinas/imunologia
Dermatite Atópica/induzido quimicamente
Dermatite Atópica/tratamento farmacológico
Dermatite Atópica/imunologia
Dermatite Atópica/patologia
Epiderme/imunologia
Epiderme/patologia
Camundongos
Camundongos Endogâmicos BALB C
NF-kappa B/imunologia
Pomadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzamides); 0 (Cyclohexanones); 0 (Cytokines); 0 (NF-kappa B); 0 (Ointments); 0 (dehydroxymethylepoxyquinomicin); 15646-46-5 (Oxazolone); GE3IBT7BMN (Dinitrochlorobenzene)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1080/08923973.2017.1312436


  9 / 2251 MEDLINE  
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[PMID]:28265582
[Au] Autor:Muñoz FC; Cervantes MM; Cervantes-García D; Jiménez M; Ventura-Juárez J; Salinas E
[Ad] Endereço:Department of Microbiology, Basic Science Center, Autonomous University of Aguascalientes, Av. Universidad No. 940, 20131 Aguascalientes, AGS, Mexico.
[Ti] Título:Glycomacropeptide Attenuates Inflammation, Pruritus, and Th2 Response Associated with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in Rat.
[So] Source:J Immunol Res;2017:6935402, 2017.
[Is] ISSN:2314-7156
[Cp] País de publicação:Egypt
[La] Idioma:eng
[Ab] Resumo:Atopic dermatitis (AD) is one of the most common skin diseases, whose incidence is increasing in industrialized countries. The epicutaneous application of a hapten, such as 2,4-dinitrochlorobenzene (DNCB), evokes an experimental murine AD-like reaction. Glycomacropeptide (GMP) is a dairy bioactive peptide derived from hydrolysis of -casein by chymosin action. It has anti-inflammatory, prebiotic, and immunomodulatory effects. The present study was aimed to investigate the effect of GMP administration on DNCB-induced AD in rats. The severity of inflammatory process, pruritus, production of cytokines, and total immunoglobulin E (IgE) content were measured, and the histopathological features were analyzed. GMP reduced the intensity of inflammatory process and edema of DNCB-induced dermatitis, with a significant decrease in eosinophils recruitment and mast cells hyperplasia. In addition GMP suppressed the serum levels of total IgE and IL-4, IL-5, and IL-13 expression in AD-lesions. Besides, the levels of IL-10 were significantly increased. Remarkably, GMP administration before AD-induction abolished pruritus in dermatitis-like reactions in the rats. Taken together, these results indicate that GMP has an inhibitory effect on AD by downregulating Th2 dominant immune response, suggesting GMP as a potential effective alternative therapy for the prevention and management of AD.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/uso terapêutico
Caseínas/uso terapêutico
Dermatite Atópica/tratamento farmacológico
Dermatite Atópica/imunologia
Inflamação/tratamento farmacológico
Fragmentos de Peptídeos/uso terapêutico
Prurido/tratamento farmacológico
Células Th2/imunologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios não Esteroides/farmacologia
Dermatite Atópica/induzido quimicamente
Dermatite Atópica/fisiopatologia
Dinitroclorobenzeno
Modelos Animais de Doenças
Eosinófilos
Imunoglobulina E/sangue
Interleucina-10/sangue
Interleucina-10/genética
Interleucina-13/sangue
Interleucina-13/genética
Interleucina-4/sangue
Interleucina-4/genética
Interleucina-5/sangue
Interleucina-5/genética
Mastócitos
Ratos
Pele/imunologia
Pele/patologia
Células Th2/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Caseins); 0 (Interleukin-13); 0 (Interleukin-5); 0 (Peptide Fragments); 0 (caseinomacropeptide); 130068-27-8 (Interleukin-10); 207137-56-2 (Interleukin-4); 37341-29-0 (Immunoglobulin E); GE3IBT7BMN (Dinitrochlorobenzene)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE
[do] DOI:10.1155/2017/6935402


  10 / 2251 MEDLINE  
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[PMID]:28254306
[Au] Autor:Uzuriaga-Sánchez RJ; Wong A; Khan S; Pividori MI; Picasso G; Sotomayor MD
[Ad] Endereço:Department of Analytical Chemistry, Institute of Chemistry, State University of São Paulo (UNESP), 14801-970 Araraquara, SP, Brazil; Laboratory of Physical Chemistry Research, Faculty of Science, National University of Engineering, Av. Tupac Amaru 210, Rimac, Lima, Peru.
[Ti] Título:Synthesis of a new magnetic-MIP for the selective detection of 1-chloro-2,4-dinitrobenzene, a highly allergenic compound.
[So] Source:Mater Sci Eng C Mater Biol Appl;74:365-373, 2017 May 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Molecularly imprinted polymers (MIPs) in combination with magnetic nanoparticles, in a core@shell format, were studied for selective detection of 1-chloro-2,4-dinitrobenzene (CDNB), a powerful allergenic substance. Magnetic nanoparticles were prepared by the co-precipitation method and mixed with oleic acid (OA). This material was then encapsulated in three types of hydrophobic polymeric matrix, poly-(MA-co-EDGMA), poly-(AA-co-EDGMA), and poly-(1-VN-co-EDGMA), by the mini-emulsion method. These matrices were used due to their ability to interact specifically with the functional groups of the analyte. Finally, the MIP-CDNB was obtained on the magnetic-hydrophobic surfaces using precipitation polymerization in the presence of the analyte. XRD diffraction patterns suggested the presence of magnetite in the composite and SEM analysis revealed a nanoparticle size between 10 and 18nm. Under the optimized adsorption conditions, the magnetic-MIP material showed a higher adsorption capacity (5.1mgg ) than its non-magnetic counterpart (4.2mgg ). In tests of the selectivity of the magnetic-MIP towards CDNB, α-values of 2.5 and 10.4, respectively, were obtained for dichlorophenol and o-nitrophenol, two structurally similar compounds, and no adsorption was observed for any other non-analogous analyte. The magnetic-MIP and magnetic-NIP were applied using water enriched with 0.5mgL of CDNB, achieving recovery values of 83.8(±0.8)% and 66(±1)%, respectively, revealing the suitability of the material for detection of CDNB.
[Mh] Termos MeSH primário: Alérgenos/análise
Cromatografia Líquida de Alta Pressão
Dinitroclorobenzeno/análise
Nanopartículas de Magnetita/química
Impressão Molecular
Polímeros/química
[Mh] Termos MeSH secundário: Microscopia Eletrônica de Varredura
Microscopia Eletrônica de Transmissão
Ácido Oleico/química
Tamanho da Partícula
Polímeros/síntese química
Porosidade
Reprodutibilidade dos Testes
Difração de Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (Magnetite Nanoparticles); 0 (Polymers); 2UMI9U37CP (Oleic Acid); GE3IBT7BMN (Dinitrochlorobenzene)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170614
[Lr] Data última revisão:
170614
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE



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