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[PMID]:26474011
[Au] Autor:Way JS; Shen Y; Martinez DS
[Ad] Endereço:Department of Medicine, University of California, Los Angeles, Los Angeles, CA jway@mednet.ucla.edu Department of Medicine, University of California, Los Angeles, Los Angeles, CA.
[Ti] Título:Iopanoic Acid to Treat Acute Psychiatric Crisis Associated With Thyrotoxicosis: Three Case Reports and Review of the Literature.
[So] Source:J Clin Psychopharmacol;35(6):743-5, 2015 Dec.
[Is] ISSN:1533-712X
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Ácido Iopanoico/farmacologia
Transtornos Mentais/etiologia
Tireotoxicose/complicações
Tireotoxicose/tratamento farmacológico
[Mh] Termos MeSH secundário: Adulto
Feminino
Seres Humanos
Ácido Iopanoico/administração & dosagem
Masculino
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; LETTER; REVIEW
[Nm] Nome de substância:
FE9794P71J (Iopanoic Acid)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151103
[Lr] Data última revisão:
151103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151017
[St] Status:MEDLINE
[do] DOI:10.1097/JCP.0000000000000418


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[PMID]:26329282
[Au] Autor:Nguyen TT; Østergaard J; Gammelgaard B
[Ad] Endereço:Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark.
[Ti] Título:A method for studies on interactions between a gold-based drug and plasma proteins based on capillary electrophoresis with inductively coupled plasma mass spectrometry detection.
[So] Source:Anal Bioanal Chem;407(28):8497-503, 2015 Nov.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:An analytical method based on capillary electrophoresis (CE) and inductively coupled plasma mass spectrometry (ICP-MS) detection was developed for studies on the interaction of gold-containing drugs and plasma proteins using auranofin as example. A detection limit of 18 ng/mL of auranofin corresponding to 5.2 ng/mL Au and a precision of 1.5 % were obtained. Kinetic studies of the interaction between auranofin and protein were performed by incubation in aqueous solutions as well as 20 % human plasma at 37 °C. The reaction of auranofin with human serum albumin (HSA) and plasma proceeded fast; 50 % of un-bound auranofin disappeared within 2 and 3 min, respectively. By blocking the free cysteine (Cys-34) by iodoacetamide on HSA, it was shown that Cys-34 was the main reaction site for auranofin. By selective labeling of HSA present in 20 % human plasma with iophenoxate, it was demonstrated that HSA was the major auranofin-interacting protein in plasma. The CE-ICP-MS method is proposed as a novel approach for kinetic studies of the interactions between gold-based drugs and plasma proteins. Graphical Abstract Development of a CE-ICP-MS based method allows for studies on interaction of the gold containing drug auranofin with plasma proteins.
[Mh] Termos MeSH primário: Antirreumáticos/sangue
Auranofina/sangue
Eletroforese Capilar/métodos
Ouro/química
Albumina Sérica/química
Espectrofotometria Atômica/métodos
[Mh] Termos MeSH secundário: Antirreumáticos/química
Auranofina/química
Cisteína/química
Seres Humanos
Iodoacetamida/química
Ácido Iopanoico/química
Cinética
Limite de Detecção
Albumina Sérica/antagonistas & inibidores
Albumina Sérica/metabolismo
Coloração e Rotulagem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antirheumatic Agents); 0 (Serum Albumin); 3H04W2810V (Auranofin); 73TJC7JGUY (iophenoxic acid); 7440-57-5 (Gold); FE9794P71J (Iopanoic Acid); K848JZ4886 (Cysteine); ZRH8M27S79 (Iodoacetamide)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:151106
[Lr] Data última revisão:
151106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150903
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-015-8997-3


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[PMID]:25962824
[Au] Autor:Renko K; Schäche S; Hoefig CS; Welsink T; Schwiebert C; Braun D; Becker NP; Köhrle J; Schomburg L
[Ad] Endereço:1 Institut für Experimentelle Endokrinologie, Charité - Universitätsmedizin Berlin , Berlin, Germany .
[Ti] Título:An Improved Nonradioactive Screening Method Identifies Genistein and Xanthohumol as Potent Inhibitors of Iodothyronine Deiodinases.
[So] Source:Thyroid;25(8):962-8, 2015 Aug.
[Is] ISSN:1557-9077
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Deiodinases (DIO1, 2, and 3) are key enzymes in thyroid hormone (TH) activation and inactivation with impact on energy metabolism, development, cell differentiation, and a number of other physiological processes. The three DIO isoenzymes thus constitute sensitive rate-limiting components within the TH axis, prone to dysregulation by endocrine disruptive compounds or disease state. In animal models and cell culture experiments, they serve as readout for local TH status and disarrangement of the hormonal axis. Furthermore, some human diseases are characterized by apparent deiodinase dysregulation (e.g., the low triiodothyronine syndrome in critical illness). Consequently, these enzymes are targets of interest for the development of pharmacological compounds with modulatory activities. Until now, the portfolio of inhibitors for these enzymes is limited. In the clinics, the DIO1-specific inhibitor propylthiouracil is in use for treatment of severe hyperthyroidism. Other well-known inhibitors (e.g., iopanoic acid or aurothioglucose) are nonselective and block all three isoenzymes. Furthermore, DIO3 was shown to be a potential oncogenic gene, which is strongly expressed in some tumors and might, in consequence, protect tumor tissue form differentiation by TH. With respect to its role in tumorigenesis, specific inhibitors of DIO3 as a potential target for anticancer drugs would be highly desirable. To this end, a flexible and convenient assay for high-throughput screening is needed. We recently described a nonradioactive screening assay, utilizing the classic Sandell-Kolthoff reaction as readout for iodide release from the substrate molecules. While we used murine liver as enzyme source, the assay was limited to murine DIO1 activity testing. Here, we describe the use of recombinant proteins as enzyme sources within the assay, expanding its suitability from murine Dio1 to human DIO1, DIO2, and DIO3. METHODS: As proof-of-concept, deiodination reactions catalyzed by these recombinant enzymes were monitored with various nonradioactive substrates and confirmed by liquid chromatography-tandem mass spectrometry. RESULTS: The contrast agent and known DIO inhibitor iopanoic acid was characterized as readily accepted substrate by DIO2 and Dio3. In a screening approach using established endocrine disrupting compounds, the natural food ingredient genistein was identified as a further DIO1-specific inhibitor, while xanthohumol turned out to potently block the activity of all three isoenzymes. CONCLUSIONS: A rapid nonradioactive screening method based on the Sandell-Kolthoff reaction is suitable for identification of environmental, nutritive and pharmacological compounds modulating activities of human deiodinase enzymes.
[Mh] Termos MeSH primário: Flavonoides/uso terapêutico
Genisteína/uso terapêutico
Iodeto Peroxidase/antagonistas & inibidores
Propiofenonas/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Catálise
Diferenciação Celular
Cromatografia Líquida
Proteínas de Ligação a DNA/química
Avaliação Pré-Clínica de Medicamentos
Enzimas/química
Células HEK293
Seres Humanos
Concentração Inibidora 50
Iodeto Peroxidase/química
Ácido Iopanoico/química
Isoenzimas/química
Espectrometria de Massas
Camundongos
Fases de Leitura Aberta
Proteínas Recombinantes/química
Hormônios Tireóideos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DIDO1 protein, human); 0 (DNA-Binding Proteins); 0 (Enzymes); 0 (Flavonoids); 0 (Isoenzymes); 0 (Propiophenones); 0 (Recombinant Proteins); 0 (Thyroid Hormones); DH2M523P0H (Genistein); EC 1.11.1.- (iodothyronine deiodinase type II); EC 1.11.1.- (iodothyronine deiodinase type III); EC 1.11.1.8 (Iodide Peroxidase); EC 1.11.1.8 (selenodeiodinase type 1, mouse); FE9794P71J (Iopanoic Acid); T4467YT1NT (xanthohumol)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150806
[Lr] Data última revisão:
150806
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150513
[St] Status:MEDLINE
[do] DOI:10.1089/thy.2015.0058


  4 / 410 MEDLINE  
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[PMID]:25913150
[Au] Autor:Bhumika S; Lemmens K; Vancamp P; Moons L; Darras VM
[Ad] Endereço:Laboratory of Comparative Endocrinology, Division Animal Physiology and Neurobiology, Biology Department, KU Leuven, B-3000 Leuven, Belgium.
[Ti] Título:Decreased thyroid hormone signaling accelerates the reinnervation of the optic tectum following optic nerve crush in adult zebrafish.
[So] Source:Mol Cell Neurosci;68:92-102, 2015 Sep.
[Is] ISSN:1095-9327
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The regenerative capacity of the adult mammalian central nervous system (CNS) is poor and finding ways to stimulate long distance axonal regeneration in humans remains a challenge for neuroscientists. Thyroid hormones, well known for their key function in CNS development and maturation, more recently also emerged as molecules influencing regeneration. While several studies investigated their influence on peripheral nerve regeneration, in vivo studies on their role in adult CNS regeneration remain scarce. We therefore investigated the effect of lowering T3 signaling on the regeneration of the optic nerve (ON) following crush in zebrafish, a species where full recovery occurs spontaneously. Adult zebrafish were exposed to iopanoic acid (IOP), which lowered intracellular 3,5,3'-triiodothyronine (T3) availability, or to the thyroid hormone receptor ß antagonist methylsulfonylnitrobenzoate (C1). Both treatments accelerated optic tectum (OT) reinnervation. At 7days post injury (7dpi) there was a clear increase in the biocytin labeled area in the OT following anterograde tracing as well as an increased immunostaining of Gap43, a protein expressed in outgrowing axons. This effect was attenuated by T3 supplementation to IOP-treated fish. ON crush induced very limited cell death and proliferation at the level of the retina in control, IOP- and C1-treated fish. The treatments also had no effect on the mRNA upregulation of the regeneration markers gap43, tub1a, and socs3b at the level of the retina at 4 and 7dpi. We did, however, find a correlation between the accelerated OT reinnervation and a more rapid resolution of microglia/macrophages in the ON and the OT of IOP-treated fish. Taken together these data indicate that lowering T3 signaling accelerates OT reinnervation following ON crush in zebrafish and that this is accompanied by a more rapid resolution of the inflammatory response.
[Mh] Termos MeSH primário: Regeneração Nervosa/fisiologia
Traumatismos do Nervo Óptico/fisiopatologia
Transdução de Sinais/fisiologia
Colículos Superiores/fisiologia
Hormônios Tireóideos/metabolismo
[Mh] Termos MeSH secundário: Animais
Encéfalo/efeitos dos fármacos
Encéfalo/metabolismo
Encéfalo/patologia
Proliferação Celular/efeitos dos fármacos
Modelos Animais de Doenças
Regulação da Expressão Gênica/efeitos dos fármacos
Compostos Heterocíclicos com 1 Anel/uso terapêutico
Antagonistas de Hormônios/farmacologia
Ácido Iopanoico/uso terapêutico
Lisina/análogos & derivados
Lisina/metabolismo
Regeneração Nervosa/efeitos dos fármacos
Proteínas do Tecido Nervoso/genética
Proteínas do Tecido Nervoso/metabolismo
Neurogênese/efeitos dos fármacos
Traumatismos do Nervo Óptico/tratamento farmacológico
Retina/metabolismo
Retina/patologia
Transdução de Sinais/efeitos dos fármacos
Colículos Superiores/efeitos dos fármacos
Hormônios Tireóideos/genética
Hormônios Tireóideos/uso terapêutico
Fatores de Tempo
Tirosina 3-Mono-Oxigenase/genética
Tirosina 3-Mono-Oxigenase/metabolismo
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Heterocyclic Compounds, 1-Ring); 0 (Hormone Antagonists); 0 (Nerve Tissue Proteins); 0 (Thyroid Hormones); 73TJC7JGUY (iophenoxic acid); EC 1.14.16.2 (Tyrosine 3-Monooxygenase); FE9794P71J (Iopanoic Acid); G6D6147J22 (biocytin); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:151005
[Lr] Data última revisão:
151005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150428
[St] Status:MEDLINE


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[PMID]:25689204
[Au] Autor:Rahman MM; Khan M; Gruber D
[Ad] Endereço:Mohammad M. Rahman and DeAnn Gruber are with the STD/HIV Program, Louisiana Office of Public Health, New Orleans. Mahmud Khan is with the Department of Health Services Policy and Management, University of South Carolina, Columbia.
[Ti] Título:A Low-Cost Partner Notification Strategy for the Control of Sexually Transmitted Diseases: A Case Study From Louisiana.
[So] Source:Am J Public Health;105(8):1675-80, 2015 Aug.
[Is] ISSN:1541-0048
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: We estimated the costs and effectiveness of implementing a partner notification (PN) strategy for highly prevalent sexually transmitted diseases (STDs) within the Louisiana STD/HIV Program. METHODS: We carried out a telephone-based PN approach on an experimental basis in 2 public STD clinics in Louisiana from June 2010 to May 2012. We monitored data on the resources used for identifying, tracing, treating, and managing the infected cases and their partners to estimate the intervention costs. RESULTS: Our results indicated that implementation of telephone-based PN should not increase the STD control program's expenses by more than 4.5%. This low-cost PN approach could successfully identify and treat 1 additional infected case at a cost of only $171. We found that the cost per disability-adjusted life year averted (a health outcome measure), because of the adoption of selective screening with partner tracing, was $4499. This was significantly lower than the gross domestic product per capita of the United States, a threshold used for defining highly cost-effective health interventions. CONCLUSIONS: Adoption of PN for gonorrhea and chlamydia should be considered a national strategy for prevention and control of these diseases.
[Mh] Termos MeSH primário: Busca de Comunicante/métodos
Doenças Sexualmente Transmissíveis/prevenção & controle
[Mh] Termos MeSH secundário: Infecções por Chlamydia/prevenção & controle
Infecções por Chlamydia/transmissão
Busca de Comunicante/economia
Análise Custo-Benefício
Gonorreia/prevenção & controle
Gonorreia/transmissão
Custos de Cuidados de Saúde
Seres Humanos
Ácido Iopanoico
Louisiana
Estudos de Casos Organizacionais
Doenças Sexualmente Transmissíveis/transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
FE9794P71J (Iopanoic Acid)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:150218
[St] Status:MEDLINE
[do] DOI:10.2105/AJPH.2014.302434


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[PMID]:25650002
[Au] Autor:Dersch JM; Nguyen TT; Østergaard J; Stürup S; Gammelgaard B
[Ad] Endereço:Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark.
[Ti] Título:Selective analysis of human serum albumin based on SEC-ICP-MS after labelling with iophenoxic acid.
[So] Source:Anal Bioanal Chem;407(10):2829-36, 2015 Apr.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Human serum albumin (HSA) is the most abundant protein in the human plasma. HSA has several physiological roles in the human body, including storage and transport. Owing to the predominance of albumin in plasma, HSA is often involved in the protein binding of drugs. The aim of this work was to develop a selective, quantitative method for determining albumin in plasma with the purpose of clarifying the fate of metal-based drugs in biological systems. The method can also be applied for determination of urine albumin, which is of relevance in diagnostics of kidney disease. A selective method for quantification of HSA based on labelling the protein with iophenoxic acid (IPA) was developed. Samples were subjected to size exclusion chromatography (SEC) and detection by inductively coupled plasma mass spectrometry (ICP-MS) monitoring iodine and platinum. The iodine signal for the HSA-IPA complex showed linearity in the range 1 to 250 mg L(-1). The precision was 3.7% and the accuracy 100.7% determined by analysis of a certified HSA reference material. The limit of detection (LOD) and limit of quantification (LOQ) were 0.23 and 9.79 mg L(-1), respectively. The method was applied for analysis of HSA in human plasma and urine samples and for studying the binding of cisplatin to proteins in the human plasma.
[Mh] Termos MeSH primário: Cromatografia em Gel/métodos
Ácido Iopanoico/química
Espectrometria de Massas/métodos
Albumina Sérica/análise
[Mh] Termos MeSH secundário: Albuminúria/urina
Cisplatino/metabolismo
Seres Humanos
Limite de Detecção
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Albumina Sérica/química
Albumina Sérica/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Serum Albumin); 73TJC7JGUY (iophenoxic acid); FE9794P71J (Iopanoic Acid); Q20Q21Q62J (Cisplatin)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160512
[Lr] Data última revisão:
160512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150205
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-015-8507-7


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[PMID]:24215905
[Au] Autor:Shinohara Y; Sakamoto M; Iwata N; Kishimoto J; Kuya K; Fujii S; Kaminou T; Watanabe T; Ogawa T
[Ad] Endereço:Division of Radiology, Department of Pathophysiological and Therapeutic Science, Faculty of Medicine, Tottori University, Japan shino-y@olive.plala.or.jp.
[Ti] Título:Usefulness of monochromatic imaging with metal artifact reduction software for computed tomography angiography after intracranial aneurysm coil embolization.
[So] Source:Acta Radiol;55(8):1015-23, 2014 Oct.
[Is] ISSN:1600-0455
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Recently, a newly developed fast-kV switching dual energy CT scanner with a gemstone detector generates virtual high keV images as monochromatic imaging (MI). Each MI can be reconstructed by metal artifact reduction software (MARS) to reduce metal artifact. PURPOSE: To evaluate the degree of metal artifacts reduction and vessel visualization around the platinum coils using dual energy CT with MARS. MATERIAL AND METHODS: Dual energy CT was performed using a Discovery CT750 HD scanner (GE Healthcare, Milwaukee, WI, USA). In a phantom study, we measured the mean standard deviation within regions of interest around a 10-mm-diameter platinum coil mass on MI with and without MARS. Thirteen patients who underwent CTA after endovascular embolization for cerebral aneurysm with platinum coils were included in a clinical study. We visually assessed the arteries around the platinum coil mass on MI with and without MARS. RESULTS: Each standard deviation near the coil mass on MI with MARS was significantly lower than that without MARS in a phantom study. On CTA of a clinical study, better visibility of neighboring arteries was obtained in 11 of 13 patients on MI with MARS compared to without MARS due to metal artifact reduction. CONCLUSION: Dual energy CT with MARS reduces metal artifact of platinum coils, resulting in favorable vessel visualization around the coil mass on CTA after embolization.
[Mh] Termos MeSH primário: Artefatos
Artérias Cerebrais/diagnóstico por imagem
Embolização Terapêutica/métodos
Processamento de Imagem Assistida por Computador/métodos
Aneurisma Intracraniano/terapia
Tomografia Computadorizada por Raios X/métodos
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Artéria Basilar/diagnóstico por imagem
Meios de Contraste
Feminino
Seres Humanos
Aneurisma Intracraniano/diagnóstico por imagem
Ácido Iopanoico/análogos & derivados
Masculino
Meia-Idade
Imagens de Fantasmas
Platina
Intensificação de Imagem Radiográfica/métodos
Estudos Retrospectivos
Software
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Contrast Media); 49DFR088MY (Platinum); 58051-18-6 (iopanoate glucuronide); FE9794P71J (Iopanoic Acid)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131113
[St] Status:MEDLINE
[do] DOI:10.1177/0284185113510492


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[PMID]:23001758
[Au] Autor:Sage M; Fourel I; Lahoreau J; Siat V; Berny P; Rossi S
[Ad] Endereço:Game and Wildlife Agency (Office national de chasse et de faune sauvage), Wildlife Sanitary Unit (unité sanitaire de faune), 67150 St. Benoist, France. mickael.sage@gmail.com
[Ti] Título:Iophenoxic acid derivatives as markers of oral baits to wildlife. New tools for their detection in tissues of a game species and safety considerations for human exposure.
[So] Source:Environ Sci Pollut Res Int;20(5):2893-904, 2013 May.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The bait-marker iophenoxic acid (IPA) and its derivatives are increasingly used for evaluating and optimizing the cost-effectiveness of baiting campaigns on wildlife, particularly on game species such as the wild boar. We aimed to determine whether concentrations of the three main IPA derivatives ethyl, methyl and propyl-IPA measured on thoracic liquid extracts (TLE) of hunted wild boars may be representative of two exposure doses, 40 and 200 mg, from 20 to 217 days after ingestion. Then we developed a method of detection of the three IPA derivatives by LC/ESI-MS-MS in muscle and liver to evaluate the suitability of these two other tissues for monitoring the marked bait consumption and for measuring available residues in the meat of marked animals. Three semi-captive wild boars received 40 mg of each IPA derivative, three received 200 mg, and three, as controls, did not receive IPA. Blood serum was sampled 20, 197 or 217 days after IPA exposure according to animals and to the derivative. Wild boars were shot by gun after the different times of serum sampling times, and TLE, muscle and liver were sampled. Our results suggest that TLE is not a relevant tissue for quantitatively expressing IPA exposure. Due to interference, no analytical method was validated on TLE containing digestive material. On the other hand, quantifications in the muscle and particularly in the liver could discriminate wild boars that had ingested the two IPA doses from 20 days until 7 months after exposure, especially for the two long term markers ethyl and propyl-IPA. So IPA quantifications in the liver sampled on hunted animals appear to be a reliable tool for monitoring bait consumption in the field at a large scale. Nevertheless, whatever the ingested dose, ethyl- and propyl-IPA concentrations measured in the muscle and the liver of tested animals until 217 days after exposure, remained higher than 0.01 mg/kg, the Maximal Residue Limit (MRL) is recommended for molecules for which no toxicological data are available. Based on the range of IPA residues available in these two tissues, implications for humans consuming marked animals are discussed.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Exposição Ambiental
Monitoramento Ambiental/métodos
Ácido Iopanoico/metabolismo
Sus scrofa/metabolismo
Espectrometria de Massas em Tandem/métodos
Vacinas/metabolismo
[Mh] Termos MeSH secundário: Animais
Biomarcadores/sangue
Biomarcadores/metabolismo
Relação Dose-Resposta a Droga
Ingestão de Alimentos
Feminino
Seres Humanos
Fígado/metabolismo
Masculino
Carne/análise
Músculos/metabolismo
Espectrometria de Massas por Ionização por Electrospray/métodos
Fatores de Tempo
Distribuição Tecidual
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (Vaccines); 73TJC7JGUY (iophenoxic acid); FE9794P71J (Iopanoic Acid)
[Em] Mês de entrada:1307
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120925
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-012-1172-x


  9 / 410 MEDLINE  
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[PMID]:22434082
[Au] Autor:Renko K; Hoefig CS; Hiller F; Schomburg L; Köhrle J
[Ad] Endereço:Institut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, D-13353 Berlin, Germany. kostja.renko@charite.de
[Ti] Título:Identification of iopanoic acid as substrate of type 1 deiodinase by a novel nonradioactive iodide-release assay.
[So] Source:Endocrinology;153(5):2506-13, 2012 May.
[Is] ISSN:1945-7170
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enzymatic 5'- and 5-deiodination are key reactions for local and systemic activation and inactivation of iodothyronines and thyronamines. Expression of the three deiodinase (DIO) isoenzymes is regulated by a number of parameters, including thyroid status, genotype, micronutrient availability, and disease-related signaling. In addition, DIO are potential targets of pharmacological as well as environmentally derived substances, which might affect their enzymatic activity (endocrine disruptors). With the classical DIO activity assay, testing depends on the availability of radioactively labeled substrates (e.g. (125)I-rT(3)) to monitor the release of radioactive iodide. Recently, liquid chromatography-tandem mass spectrometry was described as an alternative method apparently resolving this limitation. However, it has a high demand in technical equipment and analytical routine and is limited in sample number by considerable measuring time. We therefore combined the classical deiodination assay with an easily accessible photometric method taking advantage of the Sandell-Kolthoff reaction for measuring iodide release. In brief, iodine works as a catalyst within this redox reaction between Ce(4+) and As(3+) leading to an acceleration of destaining. Furthermore, the protocol was adapted to minimize handling effort and time consumption. Because this method is not dependent on radioactivity, it expands the substrate spectrum of the classical method. Suitability of this assay was tested with tissue samples from animal experiments (hepatic Dio1 activity in hypo- and hyperthyroid mice) and established DIO inhibitors. As a new but not unexpected finding, the alleged inhibitor iopanoic acid turned out to be a DIO substrate. This finding was confirmed by liquid chromatography-tandem mass spectrometry, and its potential clinical impact requires further studies.
[Mh] Termos MeSH primário: Iodeto Peroxidase/metabolismo
Ácido Iopanoico/metabolismo
Fígado/metabolismo
Glândula Tireoide/metabolismo
[Mh] Termos MeSH secundário: Animais
Camundongos
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 1.11.1.- (iodothyronine deiodinase type I); EC 1.11.1.8 (Iodide Peroxidase); FE9794P71J (Iopanoic Acid)
[Em] Mês de entrada:1206
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:120322
[St] Status:MEDLINE
[do] DOI:10.1210/en.2011-1863


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[PMID]:22115868
[Au] Autor:Kitamura K; Niinobu M; Omran AA; Takegami S; Kitade T
[Ad] Endereço:Kyoto Pharmaceutical University, 5 Nakauchicho, Misasagi, Yamashina-ku, Kyoto 607-8414, Japan. kitamura@mb.kyoto-phu.ac.jp
[Ti] Título:Binding of diuretic antihypertensive bendroflumethiazide to human serum albumin studied by ¹9F nuclear magnetic resonance method.
[So] Source:Eur J Pharm Sci;45(1-2):195-200, 2012 Jan 23.
[Is] ISSN:1879-0720
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Simultaneous specific and nonspecific binding of bendroflumethiazide (BFZ) to human serum albumin (HSA) and concentration profile of BFZ in HSA buffer (pH 7.40) solution were investigated by ¹9F nuclear magnetic resonance (NMR) method. The ¹9F NMR spectrum of BFZ (200 µM) in a buffer solution showed a sharp signal of its CF3 group at 17.8 ppm from the reference trifluoroethanol. Addition of 0.60mM HSA to the sample solution caused the CF(3) signal splitting into three broadened peaks at 18.4 (A), 17.9 (B) and 17.4 ppm (C). By its chemical shift and spectral behavior, B was assigned to unbound BFZ. Competition experiments with Site I and II ligands lead to C being assigned to Site II bound BFZ. However, the peak intensity (areas) of A was not reduced by these ligands, suggesting that A arises from nonspecific binding. Using the peak intensities at several total concentrations of BFZ, Scatchard plot was performed. The plot for A provided a straight line parallel to the x-axis confirming nonspecific binding and that for C was consistent with specific binding. The binding constants for nonspecific and specific Site II binding were 1.02 and 1.00 × 104 (M⁻¹) (n=1.1), respectively. The presence of 0.10 M Cl⁻ in the sample solution affected the binding constant of Site II binding, but not that of nonspecific binding. The concentration profile of BFZ calculated using the binding constants revealed that nonspecific binding is more effective than Site II binding for the binding of BFZ to HSA. It was also confirmed that considerable amounts of BFZ liberated from Site II by the Site II ligands or Cl⁻ ions bind again nonspecifically.
[Mh] Termos MeSH primário: Anti-Hipertensivos/metabolismo
Bendroflumetiazida/metabolismo
Diuréticos/metabolismo
Albumina Sérica/metabolismo
[Mh] Termos MeSH secundário: Anti-Hipertensivos/química
Bendroflumetiazida/química
Sítios de Ligação/efeitos dos fármacos
Ligação Competitiva/efeitos dos fármacos
Diazepam/química
Diazepam/metabolismo
Diuréticos/química
Seres Humanos
Ácido Iopanoico/análogos & derivados
Ácido Iopanoico/química
Ácido Iopanoico/metabolismo
Cinética
Ligantes
Espectroscopia de Ressonância Magnética
Concentração Osmolar
Fenilbutazona/química
Fenilbutazona/metabolismo
Ligação Proteica
Albumina Sérica/química
Albumina Sérica Humana
Triptofano/química
Triptofano/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ALB protein, human); 0 (Antihypertensive Agents); 0 (Diuretics); 0 (Ligands); 0 (Serum Albumin); 5Q52X6ICJI (Bendroflumethiazide); 73TJC7JGUY (iophenoxic acid); 8DUH1N11BX (Tryptophan); FE9794P71J (Iopanoic Acid); GN5P7K3T8S (Phenylbutazone); Q3JTX2Q7TU (Diazepam); ZIF514RVZR (Serum Albumin, Human)
[Em] Mês de entrada:1205
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111126
[St] Status:MEDLINE
[do] DOI:10.1016/j.ejps.2011.11.010



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