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[PMID]:28934709
[Au] Autor:Liu Z; Li X; Tai P; Sun L; Yuan H; Yang X
[Ad] Endereço:Key Laboratory of Pollution Ecology and Environmental Engineering, Shenyang Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
[Ti] Título:Toxicity of ammonia, cadmium, and nitrobenzene to four local fishes in the Liao River, China and the derivation of site-specific water quality criteria.
[So] Source:Ecotoxicol Environ Saf;147:656-663, 2018 Jan.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Water quality criteria (WQC) are considered to be an effective management tool for protecting aquatic environments. To derive site-specific WQC for an area, local data based on local species are essential to improve the applicability of WQC derived. Due to the paucity of local fish data available for the development of site-specific WQC for the Liao River, China, four local and widespread fishes (Pseudorasbora parva, Abbottina liaoningensis, Ctenogobius giurinus, and Misgurnus anguillicaudatus) were chosen to test their sensitivities to ammonia, cadmium and nitrobenzene. These compounds are common and regularly-measured pollutants in Chinese rivers. In addition to the published data for species resident in the Liao River, site-specific WQC for the three chemicals were derived using both a log-logistic species sensitivity distribution (SSD) and the method recommended by the USEPA, in line with current best practice, which were then compared with Chinese national WQC. It was found that A. liaoningensis was the most sensitive, followed, in order, by P. parva, C. giurinus and M. anguillicaudatus was the least sensitive, and this trend was the same to all three chemicals tested. When comparing the SSD derived solely from previously-published data with that including our data on local fish, there were significant differences identified among parameters describing the SSD curves for ammonia and nitrobenzene and significant differences were detected for site-specific WQC derived for all of the three chemicals. Based on the dataset with local fish data taxa, site-specific WQC of Liao River for ammonia, cadmium, and nitrobenzene were derived to be 20.53mg/L (at a pH of 7.0 and temperature of 20°C), 3.76µg/L (at a hardness of 100mg/L CaCO ), and 0.49mg/L, respectively. Using the same deriving method for each chemical, the national Chinese WQC were higher than site-specific WQC derived in this study for ammonia (national WQC of 25.16mg/L) and nitrobenzene (national WQC of 0.57mg/L), while the national WQC for cadmium was lower (national WQC of 1.81µg/L). These results indicated that published data can be helpful for use when deriving site-specific WQC but that there were differences between site-specific and national WQC which may lead to either over- or under-protection depending on the pollutant if national WQC were used as the basis for the water management of specific river systems, like the Liao River.
[Mh] Termos MeSH primário: Amônia/toxicidade
Cádmio/toxicidade
Peixes/crescimento & desenvolvimento
Nitrobenzenos/toxicidade
Rios/química
Poluentes Químicos da Água/toxicidade
Qualidade da Água/normas
[Mh] Termos MeSH secundário: Amônia/análise
Animais
Cádmio/análise
China
Dose Letal Mediana
Nitrobenzenos/análise
Especificidade da Espécie
Testes de Toxicidade
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrobenzenes); 0 (Water Pollutants, Chemical); 00BH33GNGH (Cadmium); 7664-41-7 (Ammonia); E57JCN6SSY (nitrobenzene)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE


  2 / 6630 MEDLINE  
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[PMID]:28463479
[Au] Autor:Hong CY; Ryu SH; Jeong H; Lee SS; Kim M; Choi IG
[Ad] Endereço:Division of Wood Chemistry & Microbiology, Department of Forest Products, National Institute of Forest Science , Seoul, Republic of Korea.
[Ti] Título:Phanerochaete chrysosporium Multienzyme Catabolic System for in Vivo Modification of Synthetic Lignin to Succinic Acid.
[So] Source:ACS Chem Biol;12(7):1749-1759, 2017 Jul 21.
[Is] ISSN:1554-8937
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Whole cells of the basidiomycete fungus Phanerochaete chrysosporium (ATCC 20696) were applied to induce the biomodification of lignin in an in vivo system. Our results indicated that P. chrysosporium has a catabolic system that induces characteristic biomodifications of synthetic lignin through a series of redox reactions, leading not only to the degradation of lignin but also to its polymerization. The reducing agents ascorbic acid and α-tocopherol were used to stabilize the free radicals generated from the ligninolytic process. The application of P. chrysosporium in combination with reducing agents produced aromatic compounds and succinic acid as well as degraded lignin polymers. P. chrysosporium selectively catalyzed the conversion of lignin to succinic acid, which has an economic value. A transcriptomic analysis of P. chrysosporium suggested that the bond cleavage of synthetic lignin was caused by numerous enzymes, including extracellular enzymes such as lignin peroxidase and manganese peroxidase, and that the aromatic compounds released were metabolized in both the short-cut and classical tricarboxylic acid cycles of P. chrysosporium. In conclusion, P. chrysosporium is suitable as a biocatalyst for lignin degradation to produce a value-added product.
[Mh] Termos MeSH primário: Lignina/metabolismo
Complexos Multienzimáticos/química
Phanerochaete/enzimologia
Ácido Succínico/síntese química
[Mh] Termos MeSH secundário: Ácido Ascórbico/química
Radicais Livres
Lignina/química
Peso Molecular
Nitrobenzenos/química
Oxirredução
Phanerochaete/metabolismo
Ácido Succínico/química
Ácido Succínico/metabolismo
Tocoferóis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Free Radicals); 0 (Multienzyme Complexes); 0 (Nitrobenzenes); 9005-53-2 (Lignin); AB6MNQ6J6L (Succinic Acid); E57JCN6SSY (nitrobenzene); PQ6CK8PD0R (Ascorbic Acid); R0ZB2556P8 (Tocopherols)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1021/acschembio.7b00046


  3 / 6630 MEDLINE  
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[PMID]:28832632
[Au] Autor:Song J; Kang HJ; Lee JW; Wenas MA; Jeong SH; Lee T; Oh K; Min KH
[Ad] Endereço:College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea.
[Ti] Título:Transetherification of 2,4-dimethoxynitrobenzene by aromatic nucleophilic substitution.
[So] Source:PLoS One;12(8):e0183575, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In view of the few reports concerning aromatic nucleophilic substitution reactions featuring an alkoxy group as a leaving group, the aromatic nucleophilic substitution of 2,4-dimethoxynitrobenzene was investigated with a bulky t-butoxide nucleophile under microwave irradiation. The transetherification of 2,4-dimethoxynitrobenezene with sodium t-butoxide under specific conditions, namely for 20 min at 110°C in 10% dimethoxyethane in toluene, afforded the desired product in 87% yield with exclusive ortho-selectivity. A variety of reaction conditions were screened to obtain the maximum yield. The aromatic nucleophilic substitution of 2,4-dimethoxynitrobenzene with t-butoxide should be carried out under controlled conditions in order to avoid the formation of byproducts, unlike that of dihalogenated activated benzenes. Among the formed byproducts, a major compound was elucidated as 2,4-dimethoxy-N-(5-methoxy-2-nitrophenyl)aniline by X-ray crystallography.
[Mh] Termos MeSH primário: Éteres/química
Nitrobenzenos/química
[Mh] Termos MeSH secundário: Espectroscopia de Ressonância Magnética Nuclear de Carbono-13
Cristalografia por Raios X
Espectroscopia de Prótons por Ressonância Magnética
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethers); 0 (Nitrobenzenes)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170824
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183575


  4 / 6630 MEDLINE  
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[PMID]:28641890
[Au] Autor:Hou Z; Lu W; Xue H; Lin J
[Ad] Endereço:Key Laboratory of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130021, China; College of Environment and Resources, Jilin University, Changchun 130021, China.
[Ti] Título:A comparative research of different ensemble surrogate models based on set pair analysis for the DNAPL-contaminated aquifer remediation strategy optimization.
[So] Source:J Contam Hydrol;203:28-37, 2017 Aug.
[Is] ISSN:1873-6009
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Surrogate-based simulation-optimization technique is an effective approach for optimizing the surfactant enhanced aquifer remediation (SEAR) strategy for clearing DNAPLs. The performance of the surrogate model, which is used to replace the simulation model for the aim of reducing computation burden, is the key of corresponding researches. However, previous researches are generally based on a stand-alone surrogate model, and rarely make efforts to improve the approximation accuracy of the surrogate model to the simulation model sufficiently by combining various methods. In this regard, we present set pair analysis (SPA) as a new method to build ensemble surrogate (ES) model, and conducted a comparative research to select a better ES modeling pattern for the SEAR strategy optimization problems. Surrogate models were developed using radial basis function artificial neural network (RBFANN), support vector regression (SVR), and Kriging. One ES model is assembling RBFANN model, SVR model, and Kriging model using set pair weights according their performance, and the other is assembling several Kriging (the best surrogate modeling method of three) models built with different training sample datasets. Finally, an optimization model, in which the ES model was embedded, was established to obtain the optimal remediation strategy. The results showed the residuals of the outputs between the best ES model and simulation model for 100 testing samples were lower than 1.5%. Using an ES model instead of the simulation model was critical for considerably reducing the computation time of simulation-optimization process and maintaining high computation accuracy simultaneously.
[Mh] Termos MeSH primário: Recuperação e Remediação Ambiental/métodos
Água Subterrânea
Hidrologia/métodos
Modelos Teóricos
Poluentes Químicos da Água
[Mh] Termos MeSH secundário: Redes Neurais (Computação)
Nitrobenzenos/análise
Tensoativos
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrobenzenes); 0 (Surface-Active Agents); 0 (Water Pollutants, Chemical); E57JCN6SSY (nitrobenzene)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170624
[St] Status:MEDLINE


  5 / 6630 MEDLINE  
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[PMID]:28629104
[Au] Autor:Chen H; Gao X; Wang C; Shao J; Xu X; Zhu L
[Ad] Endereço:Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
[Ti] Título:Efficient 2,4-dichloronitrobenzene removal in the coupled BES-UASB reactor: Effect of external voltage mode.
[So] Source:Bioresour Technol;241:879-886, 2017 Oct.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, bioelectrochemical-upflow anaerobic sludge blanket (BES-UASB) system was developed for treatment of 2,4-dichloronitrobenzen (DClNB) containing wastewater to investigate the effect of external voltage with different supplying modes. Results showed that 2,4-dichloroaniline (DClAN) was under detection limit in R1 (applied with intermittent voltage) and R2 (applied with continuous voltage) when the DClNB loading increased from 25 to 100gm d (hydraulic retention time (HRT) was decreased from 24 to 6h) while sudden accumulation of DClAN (1.7mgL ) was observed in R0 (control). Dechlorination efficiency (DE) decreased to 32.7%, 45.0% and 45.3% in R0, R1 and R2 when HRT was further shortened to 4h. Microbial community analysis indicated the significant enrichment of dechlorination-related species in R1 and R2 compared with R0, e.g., Dehalobacter and Dehalococcoides. In summary, the BES-UASB system with intermittent voltage is an alternative process for efficient treatment of DClNB containing wastewater, and the energy input was reduced markedly.
[Mh] Termos MeSH primário: Reatores Biológicos
Nitrobenzenos
Eliminação de Resíduos Líquidos
[Mh] Termos MeSH secundário: Anaerobiose
Esgotos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrobenzenes); 0 (Sewage); 85P1A896RR (2,4-dichloro-1-nitrobenzene)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE


  6 / 6630 MEDLINE  
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[PMID]:28628921
[Au] Autor:Zhuang Y; Zhao F; Liang J; Deng X; Zhang Y; Ding G; Zhang A; Jia Z; Huang S
[Ad] Endereço:Department of Nephrology, Children's Hospital of Nanjing Medical University, Nanjing, China.
[Ti] Título:Activation of COX-2/mPGES-1/PGE2 Cascade via NLRP3 Inflammasome Contributes to Albumin-Induced Proximal Tubule Cell Injury.
[So] Source:Cell Physiol Biochem;42(2):797-807, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: The activation of NOD-like receptor family, pyrin domain containing3 (NLRP3) inflammasome has been shown to be positively correlated with the severity of proteinuria in chronic kidney disease (CKD) patients. Prostaglandin E2 (PGE2), an important inflammatory mediator, is also involved in various kidney injuries. The aim of the present study was to investigate the involvement of NLRP3 inflammasome and PGE2 synthetic pathway in albumin-induced renal tubular injury. METHODS: Murine proximal tubular cells (mPTCs) were treated with albumin to induce cell injury. NLRP3 siRNA and specific COX-2 inhibitor NS398 were used to define their roles in mediating albumin-induced mPTC injury or the activation of COX-2/mPGES-1/PGE2 cascade. RESULTS: In mPCTs, inhibition of NLRP3 by a small interfering RNA (siRNA) blocked albumin-induced kidney injury molecule 1 (KIM-1) upregulation, inflammatory response, and cell apoptosis. Albumin markedly activated cyclooxygenase-2 (COX-2)/ microsomal prostaglandin E synthase-1 (mPGES-1)/PGE2 pathway in this cell line, an effect largely abolished by NLRP3 silencing at both mRNA and protein levels. More interestingly, blockade of COX-2 using a specific COX-2 inhibitor NS398 markedly inhibited the upregulation of KIM-1 and inflammatory cytokines, and attenuated cell apoptosis in line with blunted PGE2 release following albumin treatment. CONCLUSIONS: The findings suggest that COX-2/mPGES-1/PGE2 axis could be activated by albumin in the proximal tubular cells via a NLRP3 inflammasome-mediated mechanism and could thus contribute to proteinuria-related renal tubular cell injury.
[Mh] Termos MeSH primário: Ciclo-Oxigenase 2/genética
Dinoprostona/genética
Túbulos Renais Proximais/metabolismo
Proteína 3 que Contém Domínio de Pirina da Família NLR/genética
Prostaglandina-E Sintases/genética
Proteinúria/genética
[Mh] Termos MeSH secundário: Albuminas/administração & dosagem
Animais
Apoptose/genética
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Inflamassomos/genética
Inflamassomos/metabolismo
Túbulos Renais Proximais/lesões
Túbulos Renais Proximais/patologia
Camundongos
Nitrobenzenos/administração & dosagem
Proteinúria/metabolismo
Proteinúria/patologia
RNA Mensageiro/biossíntese
RNA Interferente Pequeno/genética
Sulfonamidas/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Albumins); 0 (Inflammasomes); 0 (NLR Family, Pyrin Domain-Containing 3 Protein); 0 (Nitrobenzenes); 0 (Nlrp3 protein, mouse); 0 (RNA, Messenger); 0 (RNA, Small Interfering); 0 (Sulfonamides); 123653-11-2 (N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide); EC 1.14.99.- (Ptgs2 protein, mouse); EC 1.14.99.1 (Cyclooxygenase 2); EC 5.3.99.3 (Prostaglandin-E Synthases); EC 5.3.99.3 (Ptges protein, mouse); K7Q1JQR04M (Dinoprostone)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1159/000478070


  7 / 6630 MEDLINE  
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[PMID]:28614356
[Au] Autor:Bai A; Mao C; Jenkins RW; Szulc ZM; Bielawska A; Hannun YA
[Ad] Endereço:Department of Biochemistry & Molecular Biology, Medical University of South Carolina, Charleston, South Carolina, United States of America.
[Ti] Título:Anticancer actions of lysosomally targeted inhibitor, LCL521, of acid ceramidase.
[So] Source:PLoS One;12(6):e0177805, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Acid ceramidase, which catalyzes ceramide hydrolysis to sphingosine and free fatty acid mainly in the lysosome, is being recognized as a potential therapeutic target for cancer. B13 is an effective and selective acid ceramidase inhibitor in vitro, but not as effective in cells due to poor access to the lysosomal compartment. In order to achieve targeting of B13 to the lysosome, we designed lysosomotropic N, N-dimethyl glycine (DMG)-conjugated B13 prodrug LCL521 (1,3-di-DMG-B13). Our previous results indicated the efficient delivery of B13 to the lysosome resulted in augmented effects of LCL521 on cellular acid ceramidase as evaluated by effects on substrate/product levels. Our current studies indicate that functionally, this translated into enhanced inhibition of cell proliferation. Moreover, there were greater synergistic effects of LCL521 with either ionizing radiation or Tamoxifen. Taken together, these results clearly indicate that compartmental targeting for the inhibition of acid ceramidase is an efficient and valuable therapeutic strategy.
[Mh] Termos MeSH primário: Ceramidase Ácida/antagonistas & inibidores
Antineoplásicos/síntese química
Neoplasias da Mama/enzimologia
Nitrobenzenos/química
Pró-Fármacos/síntese química
Propanolaminas/química
[Mh] Termos MeSH secundário: Antineoplásicos/química
Antineoplásicos/farmacologia
Neoplasias da Mama/tratamento farmacológico
Neoplasias da Mama/radioterapia
Proliferação Celular/efeitos dos fármacos
Proliferação Celular/efeitos da radiação
Sobrevivência Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos da radiação
Sinergismo Farmacológico
Feminino
Seres Humanos
Pró-Fármacos/química
Pró-Fármacos/farmacologia
Tamoxifeno/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (DMG-B13); 0 (Nitrobenzenes); 0 (Prodrugs); 0 (Propanolamines); 094ZI81Y45 (Tamoxifen); EC 3.5.1.23 (Acid Ceramidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177805


  8 / 6630 MEDLINE  
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[PMID]:28530292
[Au] Autor:Gundersen CB; Breedveld GD; Foseid L; Vogt RD
[Ad] Endereço:Department of Chemistry, University of Oslo, P. O. Box 1033, Blindern, NO-0315 Oslo, Norway. cathribg@kjemi.uio.no.
[Ti] Título:Soil sorption of two nitramines derived from amine-based CO capture.
[So] Source:Environ Sci Process Impacts;19(6):812-821, 2017 Jun 21.
[Is] ISSN:2050-7895
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nitramines are potentially carcinogens that form from the amines used in post-combustion CO capture (PCCC). The soil sorption characteristics of monoethanol (MEA)- and dimethyl (DMA)-nitramines have been assessed using a batch experimental setup, and defined indirectly by measuring loss of nitramine (LC-MS/MS) from the aqueous phase (0.01 M CaCl and 0.1% NaN ) after equilibrium had been established with the soil (24 h). Nitramine soil sorption was found to be strongly dependent on the content of organic matter in the soil (r = 0.72 and 0.95, p < 0.05). Soil sorption of MEA-nitramine was further influenced by the quality of the organic matter (Abs , r = 0.93, p < 0.05). This is hypothesized to be due to the hydroxyl group on the MEA-nitramine, capable of forming hydrogen bonds with acidic functional groups on the soil organic matter. Estimated organic carbon normalized soil-water distribution coefficients (K ) are relatively low, and within the same range as for simple amines. Nevertheless, considering the high content of organic matter commonly found in the top layer of a forest soil, this is where most of the nitramines will be retained. Presented data can be used to estimate final concentrations of nitramines in the environment following emissions from amine-based PCCC plants.
[Mh] Termos MeSH primário: Compostos de Anilina/análise
Dióxido de Carbono/análise
Carcinógenos/análise
Nitrobenzenos/análise
Poluentes do Solo/análise
Solo/química
[Mh] Termos MeSH secundário: Adsorção
Aminas/química
Compostos de Anilina/química
Carcinógenos/química
Cromatografia Líquida
Substâncias Húmicas/análise
Ligações de Hidrogênio
Modelos Teóricos
Nitrobenzenos/química
Poluentes do Solo/química
Espectrometria de Massas em Tandem
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amines); 0 (Aniline Compounds); 0 (Carcinogens); 0 (Humic Substances); 0 (Nitrobenzenes); 0 (Soil); 0 (Soil Pollutants); 059QF0KO0R (Water); 142M471B3J (Carbon Dioxide); GJ18911991 (nitramine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE
[do] DOI:10.1039/c7em00131b


  9 / 6630 MEDLINE  
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[PMID]:28522561
[Au] Autor:Hsu CC; Lin YS; Lin RL; Lee LY
[Ad] Endereço:Department of Physiology, University of Kentucky Medical Center, Lexington, Kentucky.
[Ti] Título:Immediate and delayed potentiating effects of tumor necrosis factor-α on TRPV1 sensitivity of rat vagal pulmonary sensory neurons.
[So] Source:Am J Physiol Lung Cell Mol Physiol;313(2):L293-L304, 2017 Aug 01.
[Is] ISSN:1522-1504
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We studied acute effects of tumor necrosis factor-α (TNFα) on the sensitivity of isolated rat vagal pulmonary sensory neurons. Our results showed the following. First, a brief pretreatment with a low dose of TNFα (1.44 nM, 9 min) enhanced the sensitivity of transient receptor potential vanilloid type 1 (TRPV1) receptors in these neurons in two distinct phases: the inward current evoked by capsaicin was amplified (Δ = 247%) immediately following the TNFα pretreatment, which gradually declined toward control and then increased again reaching another peak (Δ = 384%) after 60-90 min. Second, the immediate phase of this potentiating effect of TNFα was completely abolished by a pretreatment with a selective cyclooxygenase-2 (COX-2) inhibitor, NS-398, whereas the delayed potentiation was only partially attenuated. Third, in sharp contrast, TNFα did not generate any potentiating effect on the responses to non-TRPV1 chemical activators of these neurons. Fourth, the selectivity of the TNFα action on TRPV1 was further illustrated by the responses to acid (pH 6.0); TNFα did not affect the rapid transient current mediated by acid-sensing ion channels but significantly augmented the slow sustained current mediated by TRPV1 in the same neurons. Fifth, in anesthetized rats, a similar pattern of acute sensitizing effects of TNFα on pulmonary C-fiber afferents and the involvement of COX-2 were also clearly shown. In conclusion, a brief pretreatment with TNFα induced both immediate and delayed potentiating effects on the TRPV1 sensitivity in pulmonary sensory neurons, and the production of COX-2 arachidonic acid metabolites plays a major role in the immediate sensitizing effect of TNFα.
[Mh] Termos MeSH primário: Pulmão/metabolismo
Células Receptoras Sensoriais/metabolismo
Canais de Cátion TRPV/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
[Mh] Termos MeSH secundário: Canais Iônicos Sensíveis a Ácido/metabolismo
Animais
Capsaicina/farmacologia
Ciclo-Oxigenase 2/metabolismo
Pulmão/efeitos dos fármacos
Masculino
Fibras Nervosas Amielínicas/metabolismo
Nitrobenzenos/farmacologia
Ratos
Ratos Sprague-Dawley
Células Receptoras Sensoriais/efeitos dos fármacos
Sulfonamidas/farmacologia
Nervo Vago/efeitos dos fármacos
Nervo Vago/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acid Sensing Ion Channels); 0 (Nitrobenzenes); 0 (Sulfonamides); 0 (TRPV Cation Channels); 0 (Trpv1 protein, rat); 0 (Tumor Necrosis Factor-alpha); 123653-11-2 (N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide); EC 1.14.99.1 (Cyclooxygenase 2); S07O44R1ZM (Capsaicin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE
[do] DOI:10.1152/ajplung.00235.2016


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[PMID]:28494351
[Au] Autor:Zheng M; Gu S; Chen J; Luo Y; Li W; Ni J; Li Y; Wang Z
[Ad] Endereço:State Key Laboratory of Crop Biology, Ministry of Science and Technology, Shandong Agricultural University, Tai'an 271018, Shandong, PR China. Electronic address: zhengmengjing96@sina.com.
[Ti] Título:Development and validation of a sensitive UPLC-MS/MS instrumentation and alkaline nitrobenzene oxidation method for the determination of lignin monomers in wheat straw.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1055-1056:178-184, 2017 Jun 15.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A method to determine the lignin monomers (p-hydroxybenzaldehyde, vanillin and syringaldehyde) in plant cell wall of wheat internode was developed and validated using a high-throughput nitrobenzene oxidation step and ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for quantification. UPLC analyses were carried out using an reversed phase C column (ACQUITY UPLC BEH, 1.7µm, 2.1×100mm) and gradient elution with water and acetonitrile. This method was completely validated in terms of analyzing speed, linearity, sensitivity, limits of detection (LODs) and limits of quantification (LOQs).The three lignin monomers were successfully separated within 6min and only 2min were required to regain its equilibrium. The method linearity with regression coefficients values (R2) greater than 0.997. Additionally, LODs ranged from 0.21 to 0.89µgL and LOQs ranged from 0.69 to 2.95µgL . The applicability of this analytical approach for determining the three lignin monomers was confirmed by the successful analysis of real samples of wheat stem internodes. The nitrobenzene oxidation method was used for the analysis of lignin monomers. We have optimized the treatment temperature (170°C, 1h) and realized the high-throughput using the microwave digestion instrument. Recovery of this extraction method ranged from 68.4% to 77.7%. The analysis result showed that the guaiacyl unit (G) was the major component of lignin and there was a higher content of the syringyl unit (S) than that of the hydroxybenzyl unit (H).
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Lignina/análise
Espectrometria de Massas em Tandem/métodos
Triticum/química
[Mh] Termos MeSH secundário: Cromatografia de Fase Reversa/métodos
Limite de Detecção
Nitrobenzenos/química
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Nitrobenzenes); 9005-53-2 (Lignin); E57JCN6SSY (nitrobenzene)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE



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