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[PMID]:29431326
[Au] Autor:Khripach LV; Zheleznyak EV; Knyazeva TD; Koganova ZI; Salikhova DI; Grishin DA
[Ti] Título:[Method of colored model radicals for assessment of oxidative equilibrium in biologic samples].
[So] Source:Gig Sanit;95(9):884-90, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The most specific method of the recording of the rate offree radical reactions is the method of electron paramagnetic resonance (EPR) spectroscopy, but it is rarely used in applied biology due to expensive equipment and complexity of the execution of measurements. However chemists have found a number of colored organic radicals which lose the coloring under transition into diamagnetic form. In the given paper there are presented results of our studies on the development of methods for the assessment of oxidant equilibrium in biological media with a use of stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and cation-radicals of N,N-diethyl-p-phenylenediamine (DEPPD). We have developed the new modification of DPPH test, replacing methanol-based incubation medium by non-ionic detergent solution, compatible with native blood serum. Modified DPPH test conserved typical biphasic kinetics of the origin variant, had the similar sensitivity to model antioxidants (IC values 49, 38 and 13 mkMfor ascorbate, a-tocopherol and quercetine, correspondingly) and was applied in experiments on laboratory animals treated with nano- and ionic silver, carbon nanotubes, microfine coal and electrolytic dust. We have tried also the assay of serum lipid hydroperoxides based on Fe-initiated DEPPD oxidation (Alberti et al., 2000). The comparison of kinetics of DEPPD oxidation in model (HO/Fe) and biologic (rat serum/Fe) systems, before and after Fe addition, seems to be an evidence that ceruloplasmin (CP) was involved in the resulting process, but failed to determine its polynomial kinetics, at least for the rat serum and DEPPD excess. The use of CP monoclonal antibodies seems to be the best way for the clarification of the mechanism of this reaction.
[Mh] Termos MeSH primário: Compostos de Bifenilo
Oxirredução
Fenilenodiaminas
Picratos
Plasma
[Mh] Termos MeSH secundário: Animais
Fenômenos Bioquímicos
Compostos de Bifenilo/análise
Compostos de Bifenilo/química
Compostos de Bifenilo/metabolismo
Corantes/análise
Corantes/química
Indicadores e Reagentes/análise
Indicadores e Reagentes/química
Modelos Químicos
Fenilenodiaminas/análise
Fenilenodiaminas/química
Fenilenodiaminas/metabolismo
Picratos/análise
Picratos/química
Picratos/metabolismo
Plasma/química
Plasma/metabolismo
Ratos
Kit de Reagentes para Diagnóstico
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biphenyl Compounds); 0 (Coloring Agents); 0 (Indicators and Reagents); 0 (Phenylenediamines); 0 (Picrates); 0 (Reagent Kits, Diagnostic); 93-05-0 (N,N-diethyl 4-phenylenediamine); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE


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[PMID]:29216958
[Au] Autor:Guesmi F; Ben Hadj AS; Landoulsi A
[Ad] Endereço:Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences of Bizerte, University of Carthage, Zarzouna 7021, Tunisia.
[Ti] Título:Investigation of Extracts from Tunisian Ethnomedicinal Plants as Antioxidants, Cytotoxins, and Antimicrobials.
[So] Source:Biomed Environ Sci;30(11):811-824, 2017 Nov.
[Is] ISSN:0895-3988
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To determine the medicinal potential of various plants and their parts extracted with different solvents. METHODS: The total phenolic content of acetonitrile/water (60%-40%) (ACN/W) and aqueous (W) extract fractions was determined by high-performance liquid chromatography (HPLC), and terpenic compounds were detected by gas chromatography/mass spectrometry (GC/MS). Antioxidant activity of the samples was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and ß-carotene bleaching method. Cell viability was investigated by thiazolyl blue tetrazolium bromide [3-(4,5-dimethylthiazol)-2-yl 2,5-diphenyltetrazolium bromide] (MTT) assay. The mechanisms involved in cytotoxic activity were investigated in a murine macrophage cell line (RAW 264.7) and cancer lines. RESULTS: Our findings show that 11 plant species exhibited biological activity. In addition, moderate antibacterial activity was reported against one or more of the tested bacterial strains at two concentrations: 300 µg and 3 mg/disc. Furthermore, our data reveal that among all plants investigated, some extract and hydrophobic fractions were potent scavengers of the DPPH radical (6.78 µg/mL < EC50 < 8.55 µg/mL). Taken together, our results show that Nerium oleander (NOACN/W) and Pituranthos tortuosus (PTACN/W) were highly cytotoxic against RAW 264.7 cells with IC80 values of 0.36, and 1.55 µg/mL, respectively. In contrast, murine macrophage cell lines had low growth and were significantly sensitive to water extracts of Thymus hirtus sp. algeriensis (THW), Lavandula multifida (LMW), and ACN/W extract of Erica multiflora (EMACN/W) at doses > 400, 47.20, and 116.74 µg/mL, respectively. The current work demonstrates that RAW 264.7 cell proliferation was inhibited by samples in a dose-dependent manner. CONCLUSION: Our findings, validated through free radical scavenging activity, agar diffusion assay, and cytotoxicity of essential oils towards cancer cells, show that ethnomedicinal plants used in this work have a novel application as a tumor suppressor.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Antineoplásicos Fitogênicos/farmacologia
Citotoxinas/farmacologia
Extratos Vegetais/farmacologia
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Antineoplásicos Fitogênicos/química
Bactérias/efeitos dos fármacos
Compostos de Bifenilo
Linhagem Celular
Citotoxinas/química
Etnobotânica
Camundongos
Estrutura Molecular
Fenóis/química
Fenóis/farmacologia
Picratos
Extratos Vegetais/química
Terpenos/química
Terpenos/farmacologia
Tunísia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antineoplastic Agents, Phytogenic); 0 (Biphenyl Compounds); 0 (Cytotoxins); 0 (Phenols); 0 (Picrates); 0 (Plant Extracts); 0 (Terpenes); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.3967/bes2017.109


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[PMID]:29394011
[Au] Autor:Saladino R; Chiocchini U; Botta G; Delfino M; Conigliaro R; Mosesso P
[Ti] Título:Free radical scavenging capacity and protective effect of natural substances in peloids from the thermal spring pool Bagnaccio (Viterbo, Italy).
[So] Source:J Cosmet Sci;67(2):71-92, 2016 Mar-Apr.
[Is] ISSN:1525-7886
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Natural peloids from sulfurous thermal springs are largely used in cosmetic and pelotherapy for the treatment of different dermatological conditions, including skin aging, dermatitis, and other eczemas. The beneficial effects are correlated to mineralogical and other thermal properties, as well as to the presence of natural substances with specific antioxidant activity. Few data are available for the comparison between natural peloids and synthetic (i.e., artificially maturated) muds. In this context, the natural substances and antioxidant activity of natural white mud (WM) and dark mud (DM) peloids from the sulfurous thermal spring pool Bagnaccio (Viterbo, Italy) have been studied in detail to evaluate possible relationships between physicochemical properties and therapeutic effect. A large panel of natural substances in WM and DM were characterized for the first time by ³¹P-nuclear magnetic resonance and gas chromatography associated to mass spectrometry analysis. Polar fractions of WM and DM peloids were characterized by the presence of several bioactive natural compounds, showing high antioxidant activity and DNA protective effect, as evaluated by 2,2-diphenyl-1-picrylhydrazyl assay, and hydrogen peroxide­induced DNA breakage in the alkaline comet assay. The antioxidant activity and DNA protective effect could be attributed to radical scavenging rather than a modulatory effect on the induced DNA repair, and are of order of intensity higher than that reported for synthetic muds.
[Mh] Termos MeSH primário: Alcaloides/farmacologia
Depuradores de Radicais Livres/farmacologia
Hidrocarbonetos Aromáticos/farmacologia
Fenóis/farmacologia
Terpenos/farmacologia
[Mh] Termos MeSH secundário: Alcaloides/química
Alcaloides/isolamento & purificação
Alcanos/química
Alcanos/isolamento & purificação
Alcanos/farmacologia
Alcenos/química
Alcenos/isolamento & purificação
Alcenos/farmacologia
Animais
Compostos de Bifenilo/antagonistas & inibidores
Compostos de Bifenilo/química
Células CHO
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Ensaio Cometa
Cricetulus
DNA/efeitos dos fármacos
Depuradores de Radicais Livres/química
Depuradores de Radicais Livres/isolamento & purificação
Fontes Termais
Seres Humanos
Hidrocarbonetos Aromáticos/química
Hidrocarbonetos Aromáticos/isolamento & purificação
Peróxido de Hidrogênio/antagonistas & inibidores
Peróxido de Hidrogênio/química
Itália
Espectroscopia de Ressonância Magnética
Camundongos
Terapia por Lama
Fenóis/química
Fenóis/isolamento & purificação
Picratos/antagonistas & inibidores
Picratos/química
Terpenos/química
Terpenos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Alkanes); 0 (Alkenes); 0 (Biphenyl Compounds); 0 (Free Radical Scavengers); 0 (Hydrocarbons, Aromatic); 0 (Phenols); 0 (Picrates); 0 (Terpenes); 9007-49-2 (DNA); BBX060AN9V (Hydrogen Peroxide); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE


  4 / 5042 MEDLINE  
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[PMID]:29254300
[Au] Autor:Khalid M; Bilal M; Hassani D; Iqbal HMN; Huang D
[Ad] Endereço:School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
[Ti] Título:Antimicrobial, antioxidant, cytotoxicity and LC-MS analyses of Aerva javanica: an ethnomedicinally important plant.
[So] Source:J Biol Regul Homeost Agents;31(4):963-969, 2017 Oct-Dec.
[Is] ISSN:0393-974X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:In this study, Aerva javanica was used to extract the essential oil with notable medicinal activities. The chemical composition was investigated by high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC-MS). Ten major chemical compounds were identified as flavonoids derivatives, dihydroxylated and glycosylated metabolites. The antimicrobial, antioxidant, and cytotoxicity activities were tested using agar well-diffusion assay, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free-radical scavenging and linoleic acid oxidation assays and hemolytic assay against human erythrocytes (RBCs), respectively. Plant extracts exhibited different extents of antimicrobial activities against selected bacterial and fungal strains; however, the essential oil displayed potent antimicrobial activity against all the tested strains. The percentage inhibition of linoleic acid oxidation and inhibitory concentration (IC50) were recorded to be in the range of 42.45-96.21% and 14.21-38.18 µg/mL, respectively. Cytotoxicity profile of A. javanica extracts and essential oil was found in the range of 5.82 to 14.47%. In conclusion, A. javanica essential oil could be a potential alternative to chemical additives in the food and pharmaceutical industries.
[Mh] Termos MeSH primário: Amaranthaceae/química
Anti-Infecciosos/farmacologia
Antioxidantes/farmacologia
Citotoxinas/farmacologia
Flavonoides/farmacologia
Óleos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Anti-Infecciosos/isolamento & purificação
Antioxidantes/isolamento & purificação
Compostos de Bifenilo/antagonistas & inibidores
Compostos de Bifenilo/química
Citotoxinas/isolamento & purificação
Flavonoides/isolamento & purificação
Fungos/efeitos dos fármacos
Fungos/crescimento & desenvolvimento
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Negativas/crescimento & desenvolvimento
Bactérias Gram-Positivas/efeitos dos fármacos
Bactérias Gram-Positivas/crescimento & desenvolvimento
Concentração Inibidora 50
Ácido Linoleico/química
Testes de Sensibilidade Microbiana
Óleos Voláteis/isolamento & purificação
Oxirredução
Picratos/antagonistas & inibidores
Picratos/química
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Cytotoxins); 0 (Flavonoids); 0 (Oils, Volatile); 0 (Picrates); 0 (Plant Extracts); 9KJL21T0QJ (Linoleic Acid); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


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[PMID]:29301523
[Au] Autor:Fierascu I; Ungureanu C; Avramescu SM; Cimpeanu C; Georgescu MI; Fierascu RC; Ortan A; Sutan AN; Anuta V; Zanfirescu A; Dinu-Pirvu CE; Velescu BS
[Ad] Endereço:The National Institute for Research & Development in Chemistry and Petrochemistry, ICECHIM, 202 Spl. Independentei, 060021, Bucharest, Romania.
[Ti] Título:Genoprotective, antioxidant, antifungal and anti-inflammatory evaluation of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills.
[So] Source:BMC Complement Altern Med;18(1):3, 2018 Jan 04.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Juniperus communis L. represents a multi-purpose crop used in the pharmaceutical, food, and cosmetic industry. Several studies present the possible medicinal properties of different Juniperus taxa native to specific geographical area. The present study aims to evaluate the genoprotective, antioxidant, antifungal and anti-inflammatory potential of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian southern sub-Carpathian hills. METHODS: The prepared hydroethanolic extract of Juniperus communis L. was characterized by GC-MS, HPLC, UV-Vis spectrometry and phytochemical assays. The antioxidant potential was evaluated using the DPPH assay, the antifungal effect was studied on Aspergillus niger ATCC 15475 and Penicillium hirsutum ATCC 52323, while the genoprotective effect was evaluated using the Allium cepa assay. The anti-inflammatory effect was evaluated in two inflammation experimental models (dextran and kaolin) by plethysmometry. Male Wistar rats were treated by gavage with distilled water (negative control), the microemulsion (positive control), diclofenac sodium aqueous solution (reference) and microemulsions containing juniper extract (experimental group). The initial paw volume and the paw volumes at 1, 2, 3, 4, 5 and 24 h were measured. RESULTS: Total terpenoids, phenolics and flavonoids were estimated to be 13.44 ± 0.14 mg linalool equivalent, 19.23 ± 1.32 mg gallic acid equivalent, and 5109.6 ± 21.47 mg rutin equivalent per 100 g of extract, respectively. GC-MS characterization of the juniper extract identified 57 volatile compounds in the sample, while the HPLC analysis revealed the presence of the selected compounds (α-pinene, chlorogenic acid, rutin, apigenin, quercitin). The antioxidant potential of the crude extract was found to be 81.63 ± 0.38% (measured by the DPPH method). The results of the antifungal activity assay (for Aspergillus niger and Penicillium hirsutum) were 21.6 mm, respectively 17.2 mm as inhibition zone. Test results demonstrated the genoprotective potential of J. communis undiluted extract, inhibiting the mitodepressive effect of ethanol. The anti-inflammatory action of the juniper extract, administered as microemulsion in acute-dextran model was increased when compared to kaolin subacute inflammation induced model. CONCLUSION: The hydroalcoholic extract obtained from wild-growing Juniperus communis native to Romanian southern sub-Carpathian hills has genoprotective, antioxidant, antifungal and anti-inflammatory properties.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Antifúngicos/farmacologia
Juniperus/química
Extratos Vegetais/farmacologia
Substâncias Protetoras/farmacologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/química
Antifúngicos/química
Aspergillus niger/efeitos dos fármacos
Compostos de Bifenilo/análise
Compostos de Bifenilo/metabolismo
Flavonoides/química
Flavonoides/farmacologia
Inflamação/metabolismo
Masculino
Penicillium/efeitos dos fármacos
Fenóis/química
Fenóis/farmacologia
Picratos/análise
Picratos/metabolismo
Extratos Vegetais/química
Substâncias Protetoras/química
Ratos
Ratos Wistar
Romênia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antifungal Agents); 0 (Biphenyl Compounds); 0 (Flavonoids); 0 (Phenols); 0 (Picrates); 0 (Plant Extracts); 0 (Protective Agents); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-2066-8


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[PMID]:29295712
[Au] Autor:Ovais M; Ayaz M; Khalil AT; Shah SA; Jan MS; Raza A; Shahid M; Shinwari ZK
[Ad] Endereço:Department of Biotechnology, Quaid-i-Azam University, Islamabad, 44000, Pakistan.
[Ti] Título:HPLC-DAD finger printing, antioxidant, cholinesterase, and α-glucosidase inhibitory potentials of a novel plant Olax nana.
[So] Source:BMC Complement Altern Med;18(1):1, 2018 Jan 03.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The medicinal importance of a novel plant Olax nana Wall. ex Benth. (family: Olacaceae) was revealed for the first time via HPLC-DAD finger printing, qualitative phytochemical analysis, antioxidant, cholinesterase, and α-glucosidase inhibitory assays. METHODS: The crude methanolic extract of O. nana (ON-Cr) was subjected to qualitative phytochemical analysis and HPLC-DAD finger printing. The antioxidant potential of ON-Cr was assessed via 1,1-diphenyl,2-picrylhydrazyl (DPPH), 2,2-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS) and hydrogen peroxide (H O ) free radical scavenging assays. Furthermore, acetylcholinesterase (AChE) & butyrylcholinesterase (BChE) inhibitory activities were performed using Ellman's assay, while α- glucosidase inhibitory assay was carried out using a standard protocol. RESULTS: The qualitative phytochemical analysis of ON-Cr revealed the presence of secondary metabolites like alkaloids, flavonoids, tannins, sterols, saponins and terpenoids. The HPLC-DAD finger printing revealed the presence of 40 potential compounds in ON-Cr. Considerable anti-radical activities was revealed by ON-Cr in the DPPH, ABTS and H O free radical scavenging assays with IC values of 71.46, 72.55 and 92.33 µg/mL, respectively. Furthermore, ON-Cr showed potent AChE and BChE inhibitory potentials as indicated by their IC values of 33.2 and 55.36 µg/mL, respectively. In the α-glucosidase inhibition assay, ON-Cr exhibited moderate inhibitory propensity with an IC value of 639.89 µg/mL. CONCLUSIONS: This study investigated Olax nana for the first time for detailed qualitative phytochemical tests, HPLC-DAD finger printing analysis, antioxidant, anticholinesterase and α-glucosidase inhibition assays. The antioxidant and cholinesterase inhibitory results were considerable and can provide scientific basis for further studies on the neuroprotective and anti-Alzheimer's potentials of this plant. ON-Cr may further be subjected to fractionation and polarity guided fractionation to narrow down the search for isolation of bioactive compounds.
[Mh] Termos MeSH primário: Antioxidantes/análise
Inibidores da Colinesterase/análise
Cromatografia Líquida de Alta Pressão/métodos
Inibidores de Glicosídeo Hidrolases/análise
Olacaceae/química
Extratos Vegetais/análise
[Mh] Termos MeSH secundário: Antioxidantes/química
Antioxidantes/metabolismo
Benzotiazóis/análise
Benzotiazóis/metabolismo
Compostos de Bifenilo/análise
Compostos de Bifenilo/metabolismo
Inibidores da Colinesterase/química
Inibidores da Colinesterase/metabolismo
Inibidores de Glicosídeo Hidrolases/química
Inibidores de Glicosídeo Hidrolases/metabolismo
Peróxido de Hidrogênio/análise
Peróxido de Hidrogênio/metabolismo
Picratos/análise
Picratos/metabolismo
Extratos Vegetais/química
Extratos Vegetais/metabolismo
Ácidos Sulfônicos/análise
Ácidos Sulfônicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Benzothiazoles); 0 (Biphenyl Compounds); 0 (Cholinesterase Inhibitors); 0 (Glycoside Hydrolase Inhibitors); 0 (Picrates); 0 (Plant Extracts); 0 (Sulfonic Acids); 28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid); BBX060AN9V (Hydrogen Peroxide); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-2057-9


  7 / 5042 MEDLINE  
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[PMID]:28452569
[Au] Autor:Sengupta M; Raychaudhuri SS
[Ad] Endereço:a Department of Biophysics, Molecular Biology and Bioinformatics , University of Calcutta , Kolkata , India.
[Ti] Título:Partial alleviation of oxidative stress induced by gamma irradiation in Vigna radiata by polyamine treatment.
[So] Source:Int J Radiat Biol;93(8):803-817, 2017 08.
[Is] ISSN:1362-3095
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Environmental changes generate free radicals and reactive oxygen species (ROS) resulting in abiotic stress in plants. This causes alterations in germination, morphology, growth and development ultimately leading to yield loss. Gamma irradiation was used to experimentally induce oxidative damage in an important pulse crop Vigna radiata (L.) Wilczek or mung bean. Our research was aimed towards augmentation of oxidative stress tolerance through treatment with a group of aliphatic amines known as polyamines. MATERIALS AND METHODS: We used sub-lethal doses of gamma irradiation to generate oxidative damage which was evaluated using Nitro blue tetrazolium (NBT) staining, total antioxidant activity, 1, 1-Diphenyl-2-picryl hydrazyl (DPPH) radical scavenging assay, proline content and lipid peroxidation. Changes in internal free polyamines and messenger ribonucleic acid (mRNA) expression of key rate-limiting S-adenosylmethionine decarboxylase (SAMDC) enzyme in polyamine biosynthetic pathway was studied using real-time polymerase chain reaction (PCR). RESULTS: We observed increased oxidative damage with higher irradiation dose which was partially alleviated by putrescine treatment. Internal levels of putrescine and spermidine increased with 1 mM (50 and 100 Gy) and 2 mM putrescine treatment. Expression of SAMDC also increased with putrescine treatment. CONCLUSION: This study shows that treatment with putrescine can partially alleviate oxidative damage caused by gamma rays.
[Mh] Termos MeSH primário: Estresse Oxidativo/efeitos dos fármacos
Estresse Oxidativo/efeitos da radiação
Poliaminas/farmacologia
Vigna/metabolismo
[Mh] Termos MeSH secundário: Antioxidantes/metabolismo
Compostos de Bifenilo/metabolismo
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/efeitos da radiação
Peroxidação de Lipídeos/efeitos dos fármacos
Peroxidação de Lipídeos/efeitos da radiação
Picratos/metabolismo
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/metabolismo
Folhas de Planta/efeitos da radiação
Proteínas de Plantas/genética
Prolina/metabolismo
Vigna/efeitos dos fármacos
Vigna/genética
Vigna/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Picrates); 0 (Plant Proteins); 0 (Polyamines); 9DLQ4CIU6V (Proline); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180111
[Lr] Data última revisão:
180111
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1080/09553002.2017.1321807


  8 / 5042 MEDLINE  
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[PMID]:29221478
[Au] Autor:Shah M; Parveen Z; Khan MR
[Ad] Endereço:Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
[Ti] Título:Evaluation of antioxidant, anti-inflammatory, analgesic and antipyretic activities of the stem bark of Sapindus mukorossi.
[So] Source:BMC Complement Altern Med;17(1):526, 2017 Dec 08.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Saponins are the main constituents of genus Sapindus and have the therapeutic potential for inflammatory disorders. In this study the antioxidant, anti-inflammatory, analgesic and antipyretic potential of the stem bark of soap nut (Sapindus mukorossi) methanol extract and its derived fractions has been investigated. METHODS: Powder of stem bark of the S. mukorossi was extracted with methanol (SMM) and fractionated in order of n-hexane (SMH), chloroform (SMC), ethyl acetate (SME), n-butanol (SMB) and the remaining as aqueous fraction (SMA). Quantitative estimation for the total phenolic and total flavonoid content was carried out in all the extract/fractions. Further, various in vitro antioxidant assays were also performed. Anti-inflammatory (Carrageenan induced paw edema), analgesic (hot plate latency test) and antipyretic (rectal temperature) were determined in Sprague-Dawley rat. RESULTS: Quantitative estimation of total phenolic contents in extract/fractions varied between 252.3 ± 2.41 mg of GAE/g - 594.16 ± 4.3 mg of GAE/g while the total flavonoids estimated were from 11.02 ± 1.3 mg of RUE/g to 96.9 ± 3.2 mg of RUE/g. Standard antioxidant assays such as scavenging of DPPH, hydroxyl radical, nitric oxide, phosphomolybdenum assay, reducing power, inhibition of ß-carotene bleaching, iron chelation activity and inhibition of heat induced protein denaturation indicated the antioxidant potential of the extract/fractions. Carrageenan induced paw edema of rat was effectively inhibited by SMA at 300 mg/kg administration to rat (84.19 ± 1.48%) after 3 h and analgesia (latency time) in hot plate test (55.78 ± 1.22%) after 120 min. SMA at 300 mg/kg distinctly decreased the rectal temperature in brewer's yeast (Saccharomyces cerevisiae) induced pyrexia in rat. CONCLUSION: The resulted obtained in this study suggested the therapeutic importance of stem bark of S. mukorossi in inflammatory related disorders.
[Mh] Termos MeSH primário: Analgésicos/farmacologia
Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Antipiréticos/farmacologia
Extratos Vegetais/farmacologia
Sapindus
[Mh] Termos MeSH secundário: Analgésicos/química
Animais
Anti-Inflamatórios/química
Antioxidantes/química
Antipiréticos/química
Comportamento Animal/efeitos dos fármacos
Compostos de Bifenilo/metabolismo
Temperatura Corporal/efeitos dos fármacos
Edema
Feminino
Flavonoides/química
Flavonoides/farmacologia
Masculino
Picratos/metabolismo
Casca de Planta
Extratos Vegetais/química
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Antipyretics); 0 (Biphenyl Compounds); 0 (Flavonoids); 0 (Picrates); 0 (Plant Extracts); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171210
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-2042-3


  9 / 5042 MEDLINE  
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[PMID]:28946312
[Au] Autor:Lugonja NM; Stankovic DM; Milicic B; Spasic SD; Marinkovic V; Vrvic MM
[Ad] Endereço:Department of Chemistry, Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
[Ti] Título:Electrochemical monitoring of the breast milk quality.
[So] Source:Food Chem;240:567-572, 2018 Feb 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The electrochemical techniques were used to determine the total antioxidant capacity of breast milks and the results were compared with a commonly used spectrophotometric (2,2-diphenyl-1-picrylhydrazyl, DPPH) method. Breast milk from mothers of preterm infants was monitored in three lactation phases and after storage of expressed milk by monitoring changes in the total antioxidant capacity over a two year period. Statistical analysis showed there was no significant difference between the ability of the three methods to detect changes in breast milk after storage. Either of the electrochemical techniques studied could be successfully used to replace the time-consuming spectrophotometric method and can be applied to clinical trials.
[Mh] Termos MeSH primário: Leite Humano/química
[Mh] Termos MeSH secundário: Antioxidantes
Compostos de Bifenilo
Técnicas Eletroquímicas
Seres Humanos
Picratos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Picrates); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE


  10 / 5042 MEDLINE  
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[PMID]:28867094
[Au] Autor:Silva KDRR; Sirasa MSF
[Ad] Endereço:Department of Applied Nutrition, Faculty of Livestock, Fisheries and Nutrition, Wayamba University of Sri Lanka, Makandura, Gonawila 60170, Sri Lanka. Electronic address: renukasilva@gmail.com.
[Ti] Título:Antioxidant properties of selected fruit cultivars grown in Sri Lanka.
[So] Source:Food Chem;238:203-208, 2018 Jan 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Extracts of twenty locally available Sri Lankan fruits were analysed for 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, ferrous reducing antioxidant power (FRAP), total phenolic content (TPC), total flavonoid content (TFC) and vitamin C content. The results showed that gooseberry (Phyllanthus emblica 'local') exhibited the highest DPPH scavenging activity (111.25mg ascorbic acid equivalent antioxidant capacity (AEAC)/g), FRAP (1022.05µmol FeSO /g), TPC (915.7mg gallic acid equivalents (GAE)/100g), TFC (873.2mg catechin equivalents (CE)/100g) and vitamin C (136.8mg ascorbic acid equivalents (AAE)/100g), respectively. Sugar apple (Annona squamosa 'local') and star fruit (Averrhoa carambola 'Honey Sweet') obtained the second and third highest antioxidant activities in terms of rankings of FRAP, DPPH activities, TPC, TFC and vitamin C content. Strong correlation between vitamin C, TPC and TFC with FRAP and DPPH showed their contribution to antioxidant capacity. Among the selected fruits, underutilized fruit cultivar gooseberry showed the highest overall antioxidant potential.
[Mh] Termos MeSH primário: Frutas
[Mh] Termos MeSH secundário: Antioxidantes
Compostos de Bifenilo
Fenóis
Picratos
Extratos Vegetais
Sri Lanka
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Phenols); 0 (Picrates); 0 (Plant Extracts); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE



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