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[PMID]:28208710
[Au] Autor:Binder SB; Schwartz-Zimmermann HE; Varga E; Bichl G; Michlmayr H; Adam G; Berthiller F
[Ad] Endereço:Christian Doppler Laboratory for Mycotoxin Metabolism and Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Str. 20, 3430 Tulln, Austria. sabina.binder@boku.ac.at.
[Ti] Título:Metabolism of Zearalenone and Its Major Modified Forms in Pigs.
[So] Source:Toxins (Basel);9(2), 2017 Feb 08.
[Is] ISSN:2072-6651
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The mycotoxin zearalenone (ZEN) can be conjugated with polar molecules, like sugars or sulfates, by plants and fungi. To date, the fate of these modified forms of ZEN has not yet been elucidated in animals. In order to investigate whether ZEN conjugates contribute to the total ZEN exposure of an individual, ZEN (10 µg/kg b.w.) and equimolar amounts of two of its plant metabolites (ZEN-14- - -glucoside, ZEN-16- - -glucoside) and of one fungal metabolite (ZEN-14-sulfate) were orally administered to four pigs as a single bolus using a repeated measures design. The concentrations of ZEN, its modified forms and its mammalian metabolites ZEN-14-glucuronide, α-zearalenol (α-ZEL) and α-ZEL-14-glucuronide in excreta were analyzed by high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) based methods. The biological recovery of ZEN in urine was 26% ± 10%, the total biological recovery in excreta was 40% ± 8%. Intact ZEN-14-sulfate, ZEN-14- - -glucoside and ZEN-16- - -glucoside were neither detected in urine nor in feces. After ZEN-14-sulfate application, 19% ± 5% of the administered dose was recovered in urine. In feces, no ZEN metabolites were detected. The total biological recoveries of ZEN-14- - -glucoside and ZEN-16- - -glucoside in the form of their metabolites in urine were 19% ± 11% and 13% ± 7%, respectively. The total biological recoveries in urine and feces amounted to 48% ± 7% and 34 ± 3%. An explanation for the low biological recoveries could be extensive metabolization by intestinal bacteria to yet unknown metabolites. In summary, ZEN-14-sulfate, ZEN-14- - -glucoside, and ZEN-16- - -glucoside were completely hydrolyzed in the gastrointestinal tract of swine, thus contributing to the overall toxicity of ZEN.
[Mh] Termos MeSH primário: Microbiologia de Alimentos
Zearalenona/metabolismo
[Mh] Termos MeSH secundário: Administração Oral
Animais
Cromatografia Líquida de Alta Pressão
Fezes/química
Glucosídeos/metabolismo
Glucuronídeos/metabolismo
Hidrólise
Absorção Intestinal
Eliminação Intestinal
Masculino
Desintoxicação Metabólica Fase II
Eliminação Renal
Sus scrofa
Espectrometria de Massas em Tandem
Zearalenona/administração & dosagem
Zearalenona/análogos & derivados
Zearalenona/toxicidade
Zearalenona/urina
Zeranol/análogos & derivados
Zeranol/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucosides); 0 (Glucuronides); 0 (zearalenol); 0 (zearalenone-14-glucuronide); 132505-04-5 (zearalenone-4-sulfate); 5W827M159J (Zearalenone); 76LO2L2V39 (Zeranol)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE


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[PMID]:27921366
[Au] Autor:Gratz SW; Dinesh R; Yoshinari T; Holtrop G; Richardson AJ; Duncan G; MacDonald S; Lloyd A; Tarbin J
[Ad] Endereço:Rowett Institute of Nutrition and Health, University of Aberdeen, UK.
[Ti] Título:Masked trichothecene and zearalenone mycotoxins withstand digestion and absorption in the upper GI tract but are efficiently hydrolyzed by human gut microbiota in vitro.
[So] Source:Mol Nutr Food Res;61(4), 2017 Apr.
[Is] ISSN:1613-4133
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:SCOPE: Cereal grains are commonly contaminated with Fusarium mycotoxins and their plant-derived masked metabolites. The fate of masked mycotoxins in the human gut is poorly understood. Here we assess the metabolism and transport of glucoside metabolites of common trichothecenes (deoxynivalenol, nivalenol, T-2 toxin) and zearalenone compounds (zearalenone, α- and ß-zearalenol) in the human gut in vitro. METHODS AND RESULTS: Masked mycotoxins were incubated with artificial digestive juices and absorption was assessed in differentiated Caco-2/TC7 cells. Colonic metabolism was studied using fecal batch cultures from five donors and mycotoxins were detected using LC-MS/MS. All masked mycotoxins were stable under upper GI tract conditions and no absorption was observed. Free trichothecenes were absorbed intact whereas free zearalenone compounds were absorbed and metabolized to undetected compounds by Caco-2/TC7 cells. Human gut microbiota efficiently hydrolyzed all masked mycotoxins. Trichothecenes were fully recovered as parent mycotoxins whereas 40-70% of zearalenone compounds were further metabolized to unknown metabolites. CONCLUSION: Our results demonstrate that masked trichothecenes will reach the colon intact to be released as parent mycotoxins by gut microbiota, hence contributing to mycotoxin exposure. Masked zearalenone compounds are metabolized by gut microbiota and epithelial cells and the identity and toxicity of metabolites remain to be determined.
[Mh] Termos MeSH primário: Microbioma Gastrointestinal
Micotoxinas/farmacologia
Tricotecenos/farmacologia
Zearalenona/farmacologia
[Mh] Termos MeSH secundário: Células CACO-2/metabolismo
Fusarium/metabolismo
Seres Humanos
Hidrólise
Toxina T-2/metabolismo
Trato Gastrointestinal Superior
Zeranol/análogos & derivados
Zeranol/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mycotoxins); 0 (Trichothecenes); 0 (zearalenol); 5W827M159J (Zearalenone); 5WOP02RM1U (nivalenol); 76LO2L2V39 (Zeranol); I3FL5NM3MO (T-2 Toxin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161207
[St] Status:MEDLINE
[do] DOI:10.1002/mnfr.201600680


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[PMID]:27889531
[Au] Autor:Ben Salem I; Boussabbeh M; Da Silva JP; Guilbert A; Bacha H; Abid-Essefi S; Lemaire C
[Ad] Endereço:Laboratory for Research on Biologically Compatible Compounds, Faculty of Dentistry, Rue Avicenne, 5019 Monastir, Tunisia; Faculty of Sciences of Bizerte, Carthage University, Tunisia.
[Ti] Título:SIRT1 protects cardiac cells against apoptosis induced by zearalenone or its metabolites α- and ß-zearalenol through an autophagy-dependent pathway.
[So] Source:Toxicol Appl Pharmacol;314:82-90, 2017 Jan 01.
[Is] ISSN:1096-0333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by several species of Fusarium in cereals and agricultural products. The major ZEN metabolites are α-zearalenol (α-ZOL) and ß-zearalenol (ß-ZOL). In the present study, we investigated the underlying mechanism of the toxicity induced by ZEN, α-ZOL and ß-ZOL in cardiac cells (H9c2). We show that treatment with ZEN or its metabolites induces the activation of the mitochondrial pathway of apoptosis as characterized by an increase in ROS generation, a loss of mitochondrial transmembrane potential (ΔΨm) and an activation of caspases. Besides, we demonstrate that these mycotoxins promote the activation of autophagy before the onset of apoptosis. Indeed, we observed that a short-time (6h) treatment with ZEN, α-ZOL or ß-ZOL, increased the level of Beclin-1 and LC3-II and induced the accumulation of the CytoID® autophagy detection probe. Moreover, the inhibition of autophagy by Chloroquine significantly increased cell death induced by ZEN, α-ZOL or ß-ZOL, suggesting that the activation of autophagy serves as a cardioprotective mechanism against these mycotoxins. In addition, we found that the inhibition (EX527) or the knockdown of SIRT1 (siRNA) significantly increased apoptosis induced by ZEN or its derivatives, whereas SIRT1 activation with RSV greatly prevents the cytotoxic effects of these mycotoxins. By contrast, when autophagy was inhibited by CQ, the activation of SIRT1 by RSV had no protection against the cardiotoxicity of ZEN or its metabolites, suggesting that SIRT1 protects cardiac cells by an autophagy-dependent pathway.
[Mh] Termos MeSH primário: Apoptose/fisiologia
Autofagia/efeitos dos fármacos
Sirtuína 1/fisiologia
Zearalenona/toxicidade
Zeranol/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Morte Celular
Linhagem Celular
Miócitos Cardíacos/efeitos dos fármacos
Miócitos Cardíacos/metabolismo
Ratos
Espécies Reativas de Oxigênio/metabolismo
Zeranol/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Reactive Oxygen Species); 0 (zearalenol); 5W827M159J (Zearalenone); 76LO2L2V39 (Zeranol); EC 3.5.1.- (Sirt1 protein, rat); EC 3.5.1.- (Sirtuin 1)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161128
[St] Status:MEDLINE


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[PMID]:27664682
[Au] Autor:Bordin K; Saladino F; Fernández-Blanco C; Ruiz MJ; Mañes J; Fernández-Franzón M; Meca G; Luciano FB
[Ad] Endereço:School of Life Sciences, Pontifícia Universidade Católica, Rua Imaculada Conceição 1155, 80215-910 Curitiba, Paraná, Brazil. Electronic address: kelianibordin@gmail.com.
[Ti] Título:Reaction of zearalenone and α-zearalenol with allyl isothiocyanate, characterization of reaction products, their bioaccessibility and bioavailability in vitro.
[So] Source:Food Chem;217:648-654, 2017 Feb 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study investigates the reduction of zearalenone (ZEA) and α-zearalenol (α-ZOL) on a solution model using allyl isothiocyanate (AITC) and also determines the bioaccessibility and bioavailability of the reaction products isolated and identified by MS-LIT. Mycotoxin reductions were dose-dependent, and ZEA levels decreased more than α-ZOL, ranging from 0.2 to 96.9% and 0 to 89.5% respectively, with no difference (p⩽0.05) between pH 4 and 7. Overall, simulated gastric bioaccessibility was higher than duodenal bioaccessibility for both mycotoxins and mycotoxin-AITC conjugates, with duodenal fractions representing ⩾63.5% of the original concentration. Simulated bioavailability of reaction products (α-ZOL/ZEA-AITC) were lower than 42.13%, but significantly higher than the original mycotoxins. The cytotoxicity of α-ZOL and ZEA in Caco-2/TC7 cells was also evaluated, with toxic effects observed at higher levels than 75µM. Further studies should be performed to evaluate the toxicity and estrogenic effect of α-ZOL/ZEA-AITC.
[Mh] Termos MeSH primário: Isotiocianatos/química
Micotoxinas/química
Zearalenona/química
Zeranol/análogos & derivados
[Mh] Termos MeSH secundário: Disponibilidade Biológica
Células CACO-2
Estrogênios não Esteroides/metabolismo
Seres Humanos
Isotiocianatos/metabolismo
Micotoxinas/metabolismo
Zearalenona/metabolismo
Zeranol/química
Zeranol/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estrogens, Non-Steroidal); 0 (Isothiocyanates); 0 (Mycotoxins); 0 (zearalenol); 5W827M159J (Zearalenone); 76LO2L2V39 (Zeranol); BN34FX42G3 (allyl isothiocyanate)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170902
[Lr] Data última revisão:
170902
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160925
[St] Status:MEDLINE


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[PMID]:27401186
[Au] Autor:Vejdovszky K; Hahn K; Braun D; Warth B; Marko D
[Ad] Endereço:Department of Food Chemistry and Toxicology, University of Vienna, Waehringer Str. 38, 1090, Vienna, Austria.
[Ti] Título:Synergistic estrogenic effects of Fusarium and Alternaria mycotoxins in vitro.
[So] Source:Arch Toxicol;91(3):1447-1460, 2017 Mar.
[Is] ISSN:1432-0738
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Mycotoxins are toxic secondary metabolites formed by various fungal species that are found as natural contaminants in food. This very heterogeneous group of compounds triggers multiple toxic mechanisms, including endocrine disruptive potential. Current risk assessment of mycotoxins, as for most chemical substances, is based on the effects of single compounds. However, concern on a potential enhancement of risks by interactions of single substances in naturally occurring mixtures has greatly increased recently. In this study, the combinatory effects of three mycoestrogens were investigated in detail. This includes the endocrine disruptors zearalenone (ZEN) and α-zearalenol (α-ZEL) produced by Fusarium fungi and alternariol (AOH), a cytotoxic and estrogenic mycotoxin formed by Alternaria species. For evaluation of effects, estrogen-dependent activation of alkaline phosphatase (AlP) and cell proliferation were tested in the adenocarcinoma cell line Ishikawa. The estrogenic potential varied among the single substances. Half maximum effect concentrations (EC50) for AlP activation were evaluated for α-ZEL, ZEN and AOH as 37 pM, 562 pM and 995 nM, respectively. All three mycotoxins were found to act as partial agonists. The majority of binary combinations, even at very low concentrations in the case of α-ZEL, showed strong synergism in the AlP assay. These potentiating phenomena of mycotoxin mixtures highlight the urgent need to incorporate combinatory effects into future risk assessment, especially when endocrine disruptors are involved. To the best of our knowledge, this study presents the first investigation on synergistic effects of mycoestrogens.
[Mh] Termos MeSH primário: Estrogênios/toxicidade
Lactonas/toxicidade
Zearalenona/toxicidade
Zeranol/análogos & derivados
[Mh] Termos MeSH secundário: Fosfatase Alcalina/metabolismo
Alternaria/química
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Sinergismo Farmacológico
Fusarium/química
Seres Humanos
Lactonas/administração & dosagem
Micotoxinas/toxicidade
Testes de Toxicidade/métodos
Zearalenona/administração & dosagem
Zeranol/administração & dosagem
Zeranol/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estrogens); 0 (Lactones); 0 (Mycotoxins); 0 (zearalenol); 5W827M159J (Zearalenone); 76LO2L2V39 (Zeranol); EC 3.1.3.1 (Alkaline Phosphatase); KN9L4260JW (alternariol)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160713
[St] Status:MEDLINE
[do] DOI:10.1007/s00204-016-1795-7


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[PMID]:28029134
[Au] Autor:Gajecka M; Zielonka L; Gajecki M
[Ad] Endereço:Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13/29, 10-718 Olsztyn, Poland. mgaja@uwm.edu.pl.
[Ti] Título:Activity of Zearalenone in the Porcine Intestinal Tract.
[So] Source:Molecules;22(1), 2016 Dec 24.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:This study demonstrates that low doses (somewhat above the No Observed Adverse Effect Level, NOAEL) of the mycoestrogen zearalenone (ZEN) and its metabolites display multispecificity towards various biological targets in gilts. The observed responses in gilts were surprising. The presence of ZEN and zearalenols (ZELs) did not evoke a response in the porcine gastrointestinal tract, which was attributed to dietary tolerance. Lymphocyte proliferation was intensified in jejunal mesenteric lymph nodes, and lymphocyte counts increased in the jejunal epithelium with time of exposure. In the distal digestive tract, fecal bacterial counts decreased, the activity of fecal bacterial enzymes and lactic acid bacteria increased, and cecal water was characterized by higher genotoxicity. The accompanying hyperestrogenism led to changes in RNA activity of selected enzymes (cytochrome P450, hydroxysteroid dehydrogenases, nitric oxide synthases) and receptors (estrogen and progesterone receptors), and it stimulated post-translational modifications which play an important role in non-genomic mechanisms of signal transmission. Hyperestrogenism influences the regulation of the host's steroid hormones (estron, estradiol and progesteron), it affects the virulence of bacterial genes encoding bacterial hydroxysteroid dehydrogenases (HSDs), and it participates in detoxification processes by slowing down intestinal activity, provoking energy deficits and promoting antiporter activity at the level of enterocytes. In most cases, hyperestrogenism fulfils all of the above roles. The results of this study indicate that low doses of ZEN alleviate inflammatory processes in the digestive system, in particular in the proximal and distal intestinal tract, and increase body weight gains in gilts.
[Mh] Termos MeSH primário: Estrogênios não Esteroides/farmacologia
Regulação da Expressão Gênica/efeitos dos fármacos
Intestino Delgado/efeitos dos fármacos
Linfócitos/efeitos dos fármacos
Zearalenona/farmacologia
[Mh] Termos MeSH secundário: Animais
Proliferação Celular
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/imunologia
Feminino
Regulação da Expressão Gênica/imunologia
Hidroxiesteroide Desidrogenases/genética
Hidroxiesteroide Desidrogenases/imunologia
Intestino Delgado/citologia
Intestino Delgado/imunologia
Linfonodos/citologia
Linfonodos/efeitos dos fármacos
Linfonodos/imunologia
Linfócitos/citologia
Linfócitos/imunologia
Óxido Nítrico Sintase/genética
Óxido Nítrico Sintase/imunologia
Receptores Estrogênicos/genética
Receptores Estrogênicos/imunologia
Receptores de Progesterona/genética
Receptores de Progesterona/imunologia
Suínos
Ganho de Peso/efeitos dos fármacos
Zeranol/análogos & derivados
Zeranol/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Estrogens, Non-Steroidal); 0 (Receptors, Estrogen); 0 (Receptors, Progesterone); 0 (zearalenol); 5W827M159J (Zearalenone); 76LO2L2V39 (Zeranol); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.1.- (Hydroxysteroid Dehydrogenases); EC 1.14.13.39 (Nitric Oxide Synthase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161229
[St] Status:MEDLINE


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[PMID]:27575595
[Au] Autor:Attalah E; Nasr YS; El-Gammal HA; Nour El-Dien FA
[Ad] Endereço:a Central Laboratory of Residue Analysis of Pesticides and Heavy Metals in Food (QCAP), Agricultural Research Center , Ministry of Agriculture and Land Reclamation , Giza , Egypt.
[Ti] Título:Optimisation and validation of a new analytical method for the determination of four natural and synthetic hormones using LC-ESI-MS/MS.
[So] Source:Food Addit Contam Part A Chem Anal Control Expo Risk Assess;33(10):1545-1556, 2016 Oct.
[Is] ISSN:1944-0057
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A rapid liquid chromatographic-tandem mass spectrometric method was developed for the simultaneous determination of four natural and synthetic hormone residues (progesterone, testosterone, trenbolone acetate and zeranol) in animal tissue samples. Sample preparation was optimised to minimise time and solvent consumption. Meat samples were mechanically homogenised and digested in a procedure that gave similar recoveries to those enzymatically hydrolysed by Helix pomatia. Efficient extraction was achieved using acidified acetonitrile (1% acetic acid). Chromatographic conditions were optimised to minimise matrix effects. Analytes were separated using a C18 column with gradient elution using ammonium formate solution in methanol (MeOH)/water (1:9) and MeOH mobile phases. Finally, residues were qualitatively and quantitatively determined by electrospray ionisation tandem mass spectrometry in multiple reaction monitoring mode. Different parameters for LC-MS/MS (e.g., declustering potential and collision energy) were optimised using API 6500QT; all analytes were measured using positive-mode electrospray ionisation (ESI ) except zeranol which was measured in negative mode (ESI ). Due to LC-MS/MS signal enhancement/suppression, the determination of hormones was based on matrix-matched standard calculations. The method was validated for the four hormones on meat samples at different fortification levels and showed accepted performance criteria according to European Commission Decision 2002/657/EC. Decision limits and detection capabilities were estimated for all analytes.
[Mh] Termos MeSH primário: Produtos Biológicos/análise
Produtos da Carne/análise
Progesterona/análise
Testosterona/análise
Acetato de Trembolona/análise
Zeranol/análise
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão
Progesterona/síntese química
Espectrometria de Massas por Ionização por Electrospray
Testosterona/síntese química
Acetato de Trembolona/síntese química
Zeranol/síntese química
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Biological Products); 3XMK78S47O (Testosterone); 4G7DS2Q64Y (Progesterone); 76LO2L2V39 (Zeranol); RUD5Y4SV0S (Trenbolone Acetate)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160831
[St] Status:MEDLINE


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[PMID]:27482692
[Au] Autor:Moriel P; Artioli LF; Piccolo MB; Marques RS; Poore MH; Cooke RF
[Ti] Título:Effects of timing of anabolic implant insertion on growth and immunity of recently weaned beef steers.
[So] Source:J Anim Sci;94(7):3051-60, 2016 Jul.
[Is] ISSN:1525-3163
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We evaluated the effects of timing of estrogenic implant insertion, relative to weaning, on growth performance and measurements of innate and humoral immunity of beef calves. On d -14, Angus × Simmental crossbred steers ( = 48; BW = 217 ± 5 kg; age = 191 ± 3 d) were stratified by BW, age, and cow parity and randomly assigned to receive no implant (NOIP) or 36 mg of zeranol on d -14, 0, or 14, relative to weaning (IP-14, IP0, and IP+14, respectively; 12 steers/treatment). From d -14 to 0, cow-calf pairs remained on a single, tall-fescue pasture with no access to concentrate supplementation. Steers were weaned on d 0, stratified by treatment and BW, and then allocated into 1 of 16 drylot pens to receive daily free-choice access to a corn silage-based diet during the preconditioning phase (d 0 to 56). Steers were vaccinated against infectious bovine rhinotracheitis (IBRV), bovine viral diarrhea virus (BVDV), and on d -27 and 0. From d 56 to 252 (postpreconditioning phase), steers remained in their respective feedlot pens and were provided free-choice access to corn silage-based growing (d 56 to 167) and finishing total mixed rations (d 168 to 252). Body weight on d 0 did not differ among treatments ( ≥ 0.29) but was greater for IP-14 and IP0 than NOIP and IP+14 steers on d 14, 42, and 56 ( ≤ 0.05). Treatment effects were not detected for G:F and DMI from d 0 to 56 ( ≥ 0.34), but ADG from d -14 to 56 was greater for IP-14 compared to NOIP ( ≤ 0.05) and intermediate for IP0 and IP+14 steers. Plasma IGF-1 concentrations were greater for IP-14 than NOIP ( ≤ 0.05) and intermediate for IP0 and IP+14 steers on d -7, 0, 14, and 21. Plasma concentrations of cortisol and haptoglobin and serum titers against BVDV types 1a and 2 did not differ among treatments from d 0 to 56 ( ≥ 0.37). However, serum IBRV titers were greater for IP+14 than NOIP, IP-14, and IP0 steers ( ≤ 0.02). On d 252, BW was greater for IP-14 and IP0 than NOIP steers ( ≤ 0.05) and intermediate for IP+14 steers, but ADG and G:F from d 57 to 252 and carcass characteristics at slaughter did not differ among treatments ( ≥ 0.16). Thus, the 36-mg zeranol implant did not elicit an inflammatory response or affect the overall vaccine response of steers (except for IBRV titers). However, growth of steers during a 56-d preconditioning period was enhanced by administering 36-mg zeranol implant 14 d before weaning, without affecting subsequent postpreconditioning growth and carcass characteristics at slaughter.
[Mh] Termos MeSH primário: Bovinos/crescimento & desenvolvimento
Vacinas Virais/imunologia
Zeranol/administração & dosagem
[Mh] Termos MeSH secundário: Ração Animal/análise
Animais
Anticorpos Antivirais/sangue
Composição Corporal
Bovinos/imunologia
Vírus da Diarreia Viral Bovina Tipo 1/imunologia
Dieta/veterinária
Esquema de Medicação
Implantes de Medicamento
Haptoglobinas/metabolismo
Hidrocortisona/sangue
Fator de Crescimento Insulin-Like I
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Drug Implants); 0 (Haptoglobins); 0 (Viral Vaccines); 67763-96-6 (Insulin-Like Growth Factor I); 76LO2L2V39 (Zeranol); WI4X0X7BPJ (Hydrocortisone)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160803
[St] Status:MEDLINE
[do] DOI:10.2527/jas.2016-0470


  9 / 502 MEDLINE  
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[PMID]:27465603
[Au] Autor:Tatay E; Font G; Ruiz MJ
[Ad] Endereço:Laboratory of Toxicology, Dep. Preventive Medicine, Faculty of Pharmacy, University of Valencia, Avda. Vicent Andrés Estellés s/n, 46100, Burjassot, Valencia, Spain. Electronic address: elena-td@hotmail.com.
[Ti] Título:Cytotoxic effects of zearalenone and its metabolites and antioxidant cell defense in CHO-K1 cells.
[So] Source:Food Chem Toxicol;96:43-9, 2016 Oct.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Zearalenone (ZEA) and its metabolites (α-zearalenol; α-ZOL, ß-zearalenol; ß-ZOL) are secondary metabolites of Fusarium fungi that produce cell injury. The present study explores mycotoxin-induced cell damage and cellular protection mechanisms in CHO-K1 cells. Cytotoxicity has been determined by reactive oxygen species (ROS) production and DNA damage. ROS production was determined using the fluorescein assay and DNA strand breakage by comet assay. Intracellular protection systems were glutathione (GSH), glutathione peroxidase (GPx), catalase (CAT) and superoxide dismutase (SOD). The results demonstrated that all mycotoxins increased the ROS levels up to 5.3-fold the control levels in CHO-K1 cells. Zearalenone metabolites, but not ZEA, increased DNA damage 43% (α-ZOL) and 28% (ß-ZOL) compared to control cells. The GSH levels decreased from 18% to 36%. The GPx and SOD activities respectively increased from 26% to 62% and from 23% to 69% in CHO-K1 cells, whereas CAT activity decreased from 14% to 52%. In addition, intracellular ROS production was induced by ZEA and its metabolites. The endogenous antioxidant system components GSH, GPx and SOD were activated against ZEA and its metabolites. These antioxidant system components thus could contribute to decrease cell injury by ZEA and its metabolites.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Dano ao DNA/efeitos dos fármacos
Estrogênios não Esteroides/farmacologia
Espécies Reativas de Oxigênio/metabolismo
Zearalenona/farmacologia
Zeranol/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Células CHO
Catalase/metabolismo
Ensaio Cometa
Cricetinae
Cricetulus
Glutationa/metabolismo
Glutationa Peroxidase/metabolismo
Immunoblotting
Oxirredução
Superóxido Dismutase/metabolismo
Zeranol/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Estrogens, Non-Steroidal); 0 (Reactive Oxygen Species); 0 (zearalenol); 5W827M159J (Zearalenone); 76LO2L2V39 (Zeranol); EC 1.11.1.6 (Catalase); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160729
[St] Status:MEDLINE


  10 / 502 MEDLINE  
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[PMID]:27224899
[Au] Autor:Zhu Y; Yao X; Leung LK
[Ad] Endereço:Food and Nutritional Sciences Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
[Ti] Título:Zeranol induces COX-2 expression through TRPC-3 activation in the placental cells JEG-3.
[So] Source:Toxicol In Vitro;35:17-23, 2016 Sep.
[Is] ISSN:1879-3177
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Transient Receptor Potential Channels (TRPs) are commonly expressed in the reproductive tissues in human. Many female reproductive processes have been associated with these TRPs. The mycotoxin zeranol or α-zearalanol is derived from fungi in the Fusarium family. Limited exposure to zeranol appears to be safe. In North America, farmers are using synthetic zeranol to promote growth in livestock. As the health risks of exposure to residual zeranol have not been determined, this practice is disallowed in the European Community. In the present study the cellular calcium levels were elevated in JEG-3 cells treated with zeranol at or above 10nM. Subsequent study indicated that expressions of TRP channels were induced. In response to the calcium flow, ERK, P38 and PKCß were activated and COX-2 expression was increased. Specific TRP inhibitors were employed to establish the connection between the ion channel activity and COX-2 expression, and TRPC-3 appeared to be the triggering mechanism. Since the involvement of COX-2 is implicated in placental development and parturition, exposure to this mycotoxin poses a potential threat to pregnant women.
[Mh] Termos MeSH primário: Ciclo-Oxigenase 2/metabolismo
Estrogênios não Esteroides/farmacologia
Micotoxinas/farmacologia
Placenta/citologia
Canais de Cátion TRPC/metabolismo
Zeranol/farmacologia
[Mh] Termos MeSH secundário: Cálcio/metabolismo
Linhagem Celular Tumoral
Ciclo-Oxigenase 2/genética
Feminino
Seres Humanos
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Gravidez
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estrogens, Non-Steroidal); 0 (Mycotoxins); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (TRPC Cation Channels); 0 (TRPC3 cation channel); 76LO2L2V39 (Zeranol); EC 1.14.99.1 (Cyclooxygenase 2); EC 1.14.99.1 (PTGS2 protein, human); EC 2.7.11.24 (Mitogen-Activated Protein Kinases); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170404
[Lr] Data última revisão:
170404
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160526
[St] Status:MEDLINE



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