Base de dados : MEDLINE
Pesquisa : D02.455.426.559.389.884 [Categoria DeCS]
Referências encontradas : 642 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 65 ir para página                         

  1 / 642 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28459543
[Au] Autor:Jensen M; Davis R
[Ad] Endereço:Stanford Genome Technology Center, Department of Biochemistry, Stanford University , Palo Alto, California 94304, United States.
[Ti] Título:RecJ 5' Exonuclease Digestion of Oligonucleotide Failure Strands: A "Green" Method of Trityl-On Purification.
[So] Source:Biochemistry;56(18):2417-2424, 2017 05 09.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Methods of error filtration and correction post-gene assembly are a major bottleneck in the synthetic biology pipeline. Current oligonucleotide purification strategies, including polyacrylamide gel electrophoresis and high-performance liquid chromatography, are often expensive and labor-intensive, give low mass recovery, and contain hazardous chemicals. To circumvent these limitations, we explored an enzymatic means of oligonucleotide purification using RecJ, which is the only known exonuclease to digest single-stranded DNA (ssDNA) in the 5' to 3' direction. As a potential application to remove failure strands generated in oligonucleotide synthesis, we found RecJ does not recognize the 5' dimethoxytrityl blocking group and could therefore be used to specifically target and digest unblocked failure strands. In combination with ssDNA binding protein (SSBP), which acts to recruit RecJ via C-terminal recognition, secondary structure formation is precluded, allowing for enhanced RecJ processivity. Using this method to purify crude trityl-on oligonucleotides, we also found on average 30 units of RecJ with 0.5 µg of SSBP digests 53 pmol of 5' hydroxylated ssDNA (60 min at 37 °C). With these parameters, the average purity is increased by 8%. As such, this novel method can be adapted to most laboratory practices, particularly those with DNA synthesis automation as a simple, inexpensive (<$4), and eco-friendly means of oligonucleotide trityl-on purification.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
DNA de Cadeia Simples/química
Proteínas de Ligação a DNA/química
Exodesoxirribonucleases/química
Química Verde
Oligonucleotídeos/isolamento & purificação
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
DNA de Cadeia Simples/genética
Proteínas de Ligação a DNA/genética
Exodesoxirribonucleases/genética
Conformação de Ácido Nucleico
Ligação Proteica
Compostos de Tritil/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Single-Stranded); 0 (DNA-Binding Proteins); 0 (Oligonucleotides); 0 (Trityl Compounds); EC 3.1.- (Exodeoxyribonucleases); EC 3.1.11.- (recJ protein, Bacteria)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171205
[Lr] Data última revisão:
171205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00010


  2 / 642 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29072708
[Au] Autor:Hjuler CT; Maolanon NN; Sauer J; Stougaard J; Thygesen MB; Jensen KJ
[Ad] Endereço:Centre for Carbohydrate Recognition and Signaling, Copenhagen University, Frederiksberg, Denmark.
[Ti] Título:Preparation of glycoconjugates from unprotected carbohydrates for protein-binding studies.
[So] Source:Nat Protoc;12(11):2411-2422, 2017 Nov.
[Is] ISSN:1750-2799
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Glycobiology, in particular the study of carbohydrate-protein interactions and the events that follow, has become an important research focus in recent decades. To study these interactions, many assays require homogeneous glycoconjugates in suitable amounts. Their synthesis is one of the methodological challenges of glycobiology. Here, we describe a versatile, three-stage protocol for the formation of glycoconjugates from unprotected carbohydrates, including those purified from natural sources, as exemplified here by rhizobial Nod factors and exopolysaccharide fragments. The first stage is to add an oligo(ethylene glycol) linker (OEG-linker) that has a terminal triphenylmethanethiol group to the reducing end of the oligosaccharide by oxime formation catalyzed by aniline. The triphenylmethyl (trityl) tag is then removed from the linker to expose a thiol (stage 2) to allow a conjugation reaction at the thiol group (stage 3). There are many possible conjugation reactions, depending on the desired application. Examples shown in this protocol are as follows: (i) coupling of the oligosaccharide to a support for surface plasmon resonance (SPR) studies, (ii) fluorescence labeling for microscale thermophoresis (MST) or bioimaging, and (iii) biotinylation for biolayer interferometry (BLI) studies. This protocol starts from unprotected carbohydrates and provides glycoconjugates in milligram amounts in just 2 d.
[Mh] Termos MeSH primário: Técnicas de Química Sintética
Glicoconjugados/síntese química
Glicômica/métodos
Lipopolissacarídeos/química
Compostos de Sulfidrila/química
Compostos de Tritil/química
[Mh] Termos MeSH secundário: Compostos de Anilina/química
Biotinilação
Catálise
Interferometria
Imagem Óptica
Oximas/química
Polietilenoglicóis/química
Ligação Proteica
Ressonância de Plasmônio de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aniline Compounds); 0 (Glycoconjugates); 0 (Lipopolysaccharides); 0 (Nod factor IV, Rhizobium meliloti); 0 (Oximes); 0 (Sulfhydryl Compounds); 0 (Trityl Compounds); 30IQX730WE (Polyethylene Glycols); SIR7XX2F1K (aniline)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171027
[St] Status:MEDLINE
[do] DOI:10.1038/nprot.2017.109


  3 / 642 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28759716
[Au] Autor:Tan X; Chen L; Song Y; Rockenbauer A; Villamena FA; Zweier JL; Liu Y
[Ad] Endereço:Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University , Tianjin 300070, P. R. China.
[Ti] Título:Thiol-Dependent Reduction of the Triester and Triamide Derivatives of Finland Trityl Radical Triggers O -Dependent Superoxide Production.
[So] Source:Chem Res Toxicol;30(9):1664-1672, 2017 Sep 18.
[Is] ISSN:1520-5010
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tetrathiatriaylmethyl (trityl) radicals have found wide biomedical applications as magnetic resonance probes. Trityl radicals and their derivatives are generally stable toward biological reducing agents such as glutathione (GSH) and ascorbate. We demonstrate that the triester (ET-03) and triamide (AT-03) derivatives of the Finland trityl radical exhibit unique reduction by thiols such as GSH and cysteine (Cys) to generate the corresponding trityl carbanions as evidenced by the loss of EPR signal and appearance of characteristic UV-vis absorbance at 644 nm under anaerobic conditions. The trityl carbanions can be quickly converted back to the original trityl radicals by oxygen (O ) in air, thus rendering the reaction between the trityl derivative and biothiol undetectable under aerobic conditions. The reduction product of O by the trityl carbanions was shown to be superoxide radical (O ) by EPR spin-trapping. Kinetic studies showed that the reaction rate constants (k) depend on the types of both trityl radicals and thiols with the order of k (0.336 M s ) > k (0.070 M s ) > k (0.032 M s ) > k (0.027 M s ). The reactivity of trityl radicals with thiols is closely related to the para-substituents of trityl radicals as well as the pK of the thiols and is further reflected by the rate of O production and consumptions of O and thiols. This novel reaction represents a new metabolic process of trityl derivatives and should be considered in the design and application of new trityl radical probes.
[Mh] Termos MeSH primário: Oxigênio/química
Compostos de Sulfidrila/química
Superóxidos/química
Compostos de Tritil/química
[Mh] Termos MeSH secundário: Ácido Ascórbico/química
Espectroscopia de Ressonância de Spin Eletrônica
Radicais Livres/química
Glutationa/química
Cinética
Oxirredução
Espectrofotometria Ultravioleta
Compostos de Tritil/síntese química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Free Radicals); 0 (Sulfhydryl Compounds); 0 (Trityl Compounds); 11062-77-4 (Superoxides); GAN16C9B8O (Glutathione); PQ6CK8PD0R (Ascorbic Acid); S88TT14065 (Oxygen)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE
[do] DOI:10.1021/acs.chemrestox.7b00086


  4 / 642 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28215509
[Au] Autor:Xu Y; Wang S; Chan HF; Liu Y; Li H; He C; Li Z; Chen M
[Ad] Endereço:State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macau, China.
[Ti] Título:Triphenylphosphonium-modified poly(ethylene glycol)-poly(ε-caprolactone) micelles for mitochondria- targeted gambogic acid delivery.
[So] Source:Int J Pharm;522(1-2):21-33, 2017 Apr 30.
[Is] ISSN:1873-3476
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Mitochondria are important targets for the intracellular delivery of drugs and DNA. For mitochondria-targeted delivery, a mitochondriotropic molecule, triphenylphosphonium (TPP), was applied to the synthesis of amphiphilic TPP-poly(ethylene glycol)-poly(ε-caprolactone) (TPP-PEG-PCL) polymers. The TPP-PEG-PCL polymer was used to prepare micelles using a solvent evaporation method for the delivery of gambogic acid (GA) (GA-TPP). The micelles were obtained with a favorable particle size of 150.07±11.71nm and an encapsulation efficiency of 80.78±1.36%, and they displayed homogeneous spherical shapes. The GA-TPP micelles exerted enhanced cytotoxic and pro-apoptotic effect against A549 cells compared to free GA and GA-loaded PEG-PCL (GA-PP) micelles, due to the inhibition of the expression of apoptosis-related proteins and promotion of caspase 3/7 and caspase 9 activity. Notably, the mitochondria-targeting GA-TPP micelles selectively accumulated in the mitochondria, inducing the loss of mitochondrial membrane potential and the release of cytochrome c, thereby achieving improved mitochondria-targeting effects. In conclusion, the GA-TPP micelle system shows great promise for lung cancer treatment by inducing an apoptotic effect via the mitochondrial signaling pathway.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/administração & dosagem
Ácido Láctico/química
Mitocôndrias/efeitos dos fármacos
Oniocompostos/química
Poliésteres/química
Ácido Poliglicólico/química
Compostos de Tritil/química
Xantonas/administração & dosagem
[Mh] Termos MeSH secundário: Células A549
Apoptose/efeitos dos fármacos
Proteínas Reguladoras de Apoptose/metabolismo
Caspases/metabolismo
Citocromos c/metabolismo
Sistemas de Liberação de Medicamentos
Seres Humanos
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Micelas
Tamanho da Partícula
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Apoptosis Regulatory Proteins); 0 (Micelles); 0 (Onium Compounds); 0 (Polyesters); 0 (Trityl Compounds); 0 (Xanthones); 0 (polylactic acid-polyglycolic acid copolymer); 15912-74-0 (triphenylmethylphosphonium); 24980-41-4 (polycaprolactone); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); 8N585K83U2 (gambogic acid); 9007-43-6 (Cytochromes c); EC 3.4.22.- (Caspases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170816
[Lr] Data última revisão:
170816
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


  5 / 642 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28192487
[Au] Autor:Pattamayutanon P; Angeli S; Thakeow P; Abraham J; Disayathanoowat T; Chantawannakul P
[Ad] Endereço:Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
[Ti] Título:Volatile organic compounds of Thai honeys produced from several floral sources by different honey bee species.
[So] Source:PLoS One;12(2):e0172099, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The volatile organic compounds (VOCs) of four monofloral and one multifloral of Thai honeys produced by Apis cerana, Apis dorsata and Apis mellifera were analyzed by headspace solid-phase microextraction (HS-SPME) followed by gas chromatography and mass spectrometry (GC-MS). The floral sources were longan, sunflower, coffee, wild flowers (wild) and lychee. Honey originating from longan had more VOCs than all other floral sources. Sunflower honey had the least numbers of VOCs. cis-Linalool oxide, trans-linalool oxide, ho-trienol, and furan-2,5-dicarbaldehyde were present in all the honeys studied, independent of their floral origin. Interestingly, 2-phenylacetaldehyde was detected in all honey sample except longan honey produced by A. cerana. Thirty-two VOCs were identified as possible floral markers. After validating differences in honey volatiles from different floral sources and honeybee species, the results suggest that differences in quality and quantity of honey volatiles are influenced by both floral source and honeybee species. The group of honey volatiles detected from A. cerana was completely different from those of A. mellifera and A. dorsata. VOCs could therefore be applied as chemical markers of honeys and may reflect preferences of shared floral sources amongst different honeybee species.
[Mh] Termos MeSH primário: Abelhas/fisiologia
Flores/química
Mel/análise
Compostos Orgânicos Voláteis/análise
[Mh] Termos MeSH secundário: Acetaldeído/análogos & derivados
Acetaldeído/análise
Acetaldeído/isolamento & purificação
Animais
Abelhas/classificação
Café/química
Cicloexanóis/análise
Cicloexanóis/isolamento & purificação
Cromatografia Gasosa-Espectrometria de Massas
Helianthus/química
Litchi/química
Monoterpenos/análise
Monoterpenos/isolamento & purificação
Sapindaceae/química
Microextração em Fase Sólida
Especificidade da Espécie
Tailândia
Compostos de Tritil/análise
Compostos de Tritil/isolamento & purificação
Compostos Orgânicos Voláteis/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coffee); 0 (Cyclohexanols); 0 (Monoterpenes); 0 (Trityl Compounds); 0 (Volatile Organic Compounds); 4UJJ55KMCS (linalool oxide); GO1N1ZPR3B (Acetaldehyde); U8J5PLW9MR (phenylacetaldehyde)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0172099


  6 / 642 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28060543
[Au] Autor:Organ L; Bacci B; Koumoundouros E; Kimpton WG; Samuel CS; Nowell CJ; Bradding P; Roach KM; Westall G; Jaffar J; Snibson KJ
[Ad] Endereço:1 Faculty of Veterinary and Agricultural Science, The University of Melbourne, Parkville, Victoria, Australia.
[Ti] Título:Inhibition of the K 3.1 Channel Alleviates Established Pulmonary Fibrosis in a Large Animal Model.
[So] Source:Am J Respir Cell Mol Biol;56(4):539-550, 2017 Apr.
[Is] ISSN:1535-4989
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive disease of increasing prevalence marked by poor prognosis and limited treatment options. Ca -activated K 3.1 potassium channels have been shown to play a key role in the aberrant activation and responses to injury in both epithelial cells and fibroblasts, both considered key drivers in the fibrotic process of IPF. Pharmacological inhibition of IPF-derived fibroblasts is able to somewhat prevent TGF-ß- and basic fibroblast growth factor-dependent profibrotic responses. In the current study, we investigated whether blockade of the K 3.1 ion channel in vivo with a selective inhibitor, Senicapoc, was able to attenuate both histological and physiological outcomes of early fibrosis in our large animal (sheep) model for pulmonary fibrosis. We also determined whether treatment was targeting the profibrotic activity of sheep lung fibroblasts. Senicapoc was administered in established fibrosis, at 2 weeks after bleomycin instillation, and drug efficacy was assessed 4 weeks after treatment. Treatment with Senicapoc improved pre-established bleomycin-induced changes compared with vehicle control, leading to improved lung compliance, reduced extracellular matrix and collagen deposition, and a reduction in both α-smooth muscle actin expression and proliferating cells, both in vivo and in vitro. These studies show that inhibiting the K 3.1 ion channel is able to attenuate the early fibrogenic phase of bleomycin-dependent fibrosis and inhibits profibrotic behavior of primary sheep lung fibroblasts. This supports the previous research conducted in human IPF-derived fibroblasts and suggests that inhibiting K 3.1 signaling may provide a novel therapeutic approach for IPF.
[Mh] Termos MeSH primário: Canais de Potássio Ativados por Cálcio de Condutância Intermediária/antagonistas & inibidores
Fibrose Pulmonar/metabolismo
[Mh] Termos MeSH secundário: Acetamidas/farmacologia
Animais
Bleomicina
Complacência (Medida de Distensibilidade)
Modelos Animais de Doenças
Fibroblastos/efeitos dos fármacos
Fibroblastos/metabolismo
Fibroblastos/patologia
Imunofluorescência
Canais de Potássio Ativados por Cálcio de Condutância Intermediária/metabolismo
Pulmão/efeitos dos fármacos
Pulmão/metabolismo
Pulmão/patologia
Pulmão/fisiopatologia
Fibrose Pulmonar/patologia
Fibrose Pulmonar/fisiopatologia
Testes de Função Respiratória
Ovinos
Compostos de Tritil/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetamides); 0 (Intermediate-Conductance Calcium-Activated Potassium Channels); 0 (Trityl Compounds); 11056-06-7 (Bleomycin); TS6G201A6Q (senicapoc)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.1165/rcmb.2016-0092OC


  7 / 642 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27998733
[Au] Autor:Ayed L; Bakir K; Ben Mansour H; Hammami S; Cheref A; Bakhrouf A
[Ad] Endereço:Laboratoire d'Analyse, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Faculté de Pharmacie, Rue Avicenne, 05000 Monastir, Tunisia; Institut Supérieur de Biotechnologie de Sfax, Route de la Soukra Km 4 BP 261, 3000 Sfax, Tunisia. Electronic address: alym712@yahoo.fr.
[Ti] Título:In vitro mutagenicity, NMR metabolite characterization of azo and triphenylmethanes dyes by adherents bacteria and the role of the "cna" adhesion gene in activated sludge.
[So] Source:Microb Pathog;103:29-39, 2017 Feb.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Staphylococcus aureus, showing the greatest decolorization ability, was further investigated for Methyl Red (MR) Congo Red (CR), Crystal Violet (CV) and Malachite Green (MG) decolorization using response surface methodology (RSM). The chemometric methods use, based on statistical design of experiments (DOEs) such as RSM is becoming increasingly widespread in several sciences such as analytical chemistry, engineering and environmental chemistry. Stapphylococcus aureus ATCC 25923, Stapphylococcus aureus (S1) and Stapphylococcus aureus (S2), were isolated from textile wastewater plant located in KsarHellal, Tunisia and were tested for their decolorization capacity. PCR technique was utilized to identify the 3 bacterial strains and to detect the adhesin gene "cna". Biodegradation of MR, CR, CV and MG (750 ppm), were investigated under shaking condition in Mineral Salt Medium (MSM) solution at pH 7.5 and temperature 30 °C, using a 3.7 × 10 CFU/ml as inoculum size. Our results showed that Staphylococcus aureus had a high decolorization capacity. Nuclear magnetic resonance (NMR) spectroscopy analysis confirmed the biodegradation of dyes. The four dyes mutagenicity with the S9 metabolizing system decreased significantly after biodegradation and totally disappeared. Nuclear magnetic resonance (NMR) spectroscopy analysis confirmed the biodegradation of dyes.
[Mh] Termos MeSH primário: Adesinas Bacterianas/genética
Compostos Azo/toxicidade
Bactérias/genética
Bactérias/metabolismo
Corantes/toxicidade
Mutação
Esgotos/microbiologia
Compostos de Tritil/toxicidade
[Mh] Termos MeSH secundário: Compostos Azo/química
Compostos Azo/metabolismo
Aderência Bacteriana/genética
Biodegradação Ambiental
Corantes/química
Corantes/metabolismo
Espectroscopia de Ressonância Magnética
Metabolômica/métodos
Mutagênese/efeitos dos fármacos
Mutagênicos/química
Mutagênicos/metabolismo
Mutagênicos/toxicidade
Staphylococcus aureus/genética
Staphylococcus aureus/metabolismo
Compostos de Tritil/química
Compostos de Tritil/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adhesins, Bacterial); 0 (Azo Compounds); 0 (Coloring Agents); 0 (Mutagens); 0 (Sewage); 0 (Trityl Compounds); 0 (adhesin, Staphylococcus aureus); 8O4UTW9E17 (triphenylmethane)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE


  8 / 642 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27876619
[Au] Autor:Staal RG; Khayrullina T; Zhang H; Davis S; Fallon SM; Cajina M; Nattini ME; Hu A; Zhou H; Poda SB; Zorn S; Chandrasena G; Dale E; Cambpell B; Biilmann Rønn LC; Munro G; MÓ§ller T
[Ad] Endereço:Neuroinflammation Disease Biology Unit, Lundbeck Research USA Inc., 215 College Rd, Paramus, NJ 07652, USA. Electronic address: staalro@yahoo.com.
[Ti] Título:Inhibition of the potassium channel K 3.1 by senicapoc reverses tactile allodynia in rats with peripheral nerve injury.
[So] Source:Eur J Pharmacol;795:1-7, 2017 Jan 15.
[Is] ISSN:1879-0712
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Neuropathic pain is a debilitating, chronic condition with a significant unmet need for effective treatment options. Recent studies have demonstrated that in addition to neurons, non-neuronal cells such as microglia contribute to the initiation and maintenance of allodynia in rodent models of neuropathic pain. The Ca - activated K channel, K 3.1 is critical for the activation of immune cells, including the CNS-resident microglia. In order to evaluate the role of K 3.1 in the maintenance of mechanical allodynia following peripheral nerve injury, we used senicapoc, a stable and highly potent K 3.1 inhibitor. In primary cultured microglia, senicapoc inhibited microglial nitric oxide and IL-1ß release. In vivo, senicapoc showed high CNS penetrance and when administered to rats with peripheral nerve injury, it significantly reversed tactile allodynia similar to the standard of care, gabapentin. In contrast to gabapentin, senicapoc achieved efficacy without any overt impact on locomotor activity. Together, the data demonstrate that the K 3.1 inhibitor senicapoc is effective at reducing mechanical hypersensitivity in a rodent model of peripheral nerve injury.
[Mh] Termos MeSH primário: Acetamidas/farmacologia
Hiperalgesia/complicações
Hiperalgesia/tratamento farmacológico
Canais de Potássio Ativados por Cálcio de Condutância Intermediária/antagonistas & inibidores
Traumatismos dos Nervos Periféricos/complicações
Bloqueadores dos Canais de Potássio/farmacologia
Compostos de Tritil/farmacologia
[Mh] Termos MeSH secundário: Acetamidas/efeitos adversos
Acetamidas/farmacocinética
Acetamidas/uso terapêutico
Animais
Células CHO
Cricetinae
Cricetulus
Relação Dose-Resposta a Droga
Estabilidade de Medicamentos
Seres Humanos
Hiperalgesia/metabolismo
Hiperalgesia/fisiopatologia
Locomoção/efeitos dos fármacos
Microglia/efeitos dos fármacos
Microglia/metabolismo
Potássio/metabolismo
Bloqueadores dos Canais de Potássio/efeitos adversos
Bloqueadores dos Canais de Potássio/farmacocinética
Bloqueadores dos Canais de Potássio/uso terapêutico
Ratos
Compostos de Tritil/efeitos adversos
Compostos de Tritil/farmacocinética
Compostos de Tritil/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetamides); 0 (Intermediate-Conductance Calcium-Activated Potassium Channels); 0 (KCNN4 protein, human); 0 (Potassium Channel Blockers); 0 (Trityl Compounds); RWP5GA015D (Potassium); TS6G201A6Q (senicapoc)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE


  9 / 642 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27622736
[Au] Autor:Souto-Maior FN; Fonsêca DV; Salgado PR; Monte LO; de Sousa DP; de Almeida RN
[Ad] Endereço:a Postgraduate Program in Natural Products and Bioactive Synthetics (PgPNSB) , Federal University of Paraíba (UFPB) , João Pessoa , Paraíba , Brazil.
[Ti] Título:Antinociceptive and anticonvulsant effects of the monoterpene linalool oxide.
[So] Source:Pharm Biol;55(1):63-67, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Linalool oxide (OXL) (a monoterpene) is found in the essential oils of certain aromatic plants, or it is derived from linalool. The motivation for this work is the lack of psychopharmacological studies on this substance. OBJECTIVE: To evaluate OXL's acute toxicity, along with its anticonvulsant and antinociceptive activities in male Swiss mice. MATERIAL AND METHODS: OXL (50, 100 and 150 mg/kg, i.p.) was investigated for acute toxicity and in the Rota-rod test. Antinociceptive activity was evaluated by the acetic acid-induced writhing test, and by formalin testing. Anticonvulsant effects were demonstrated by testing for pentylenetetrazol (PTZ)-induced seizures and by Maximum Electroshock headset (MES) test. OXL was administered to the animals intraperitoneally 30 min before for pharmacological tests. RESULTS: OXL showed an LD of ∼721 (681-765) mg/kg. In the Rota-rod test, it was observed that OXL caused no damage to the animal's motor coordination. OXL significantly reduced (p < .001) the number of writhings. OXL also significantly decreased (p < .05, p < .01 or p < .001) paw-licking time in the two phases of the formalin test. OXL significantly reduced (p < .01 or p < .001) the duration of tonic seizures in the MES test, and at the dose 150 mg/kg, significantly increased (p < .01) the latency to first seizure in the PTZ test. CONCLUSION: The tested doses of OXL were safe, with no motor impairment, and show clear antinociceptive and anticonvulsant potential. Future investigations with this monoterpene may lead to the development of a new molecule with even higher potency and selectivity.
[Mh] Termos MeSH primário: Analgésicos/farmacologia
Anticonvulsivantes/farmacologia
Comportamento Animal/efeitos dos fármacos
Cicloexanóis/farmacologia
Monoterpenos/farmacologia
Nociceptividade/efeitos dos fármacos
Dor Nociceptiva/prevenção & controle
Convulsões/prevenção & controle
Compostos de Tritil/farmacologia
[Mh] Termos MeSH secundário: Ácido Acético
Analgésicos/toxicidade
Animais
Anticonvulsivantes/toxicidade
Cicloexanóis/toxicidade
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Eletrochoque
Formaldeído
Dose Letal Mediana
Masculino
Camundongos
Monoterpenos/toxicidade
Atividade Motora
Dor Nociceptiva/induzido quimicamente
Dor Nociceptiva/fisiopatologia
Dor Nociceptiva/psicologia
Pentilenotetrazol
Tempo de Reação/efeitos dos fármacos
Teste de Desempenho do Rota-Rod
Convulsões/induzido quimicamente
Fatores de Tempo
Compostos de Tritil/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Anticonvulsants); 0 (Cyclohexanols); 0 (Monoterpenes); 0 (Trityl Compounds); 1HG84L3525 (Formaldehyde); 4UJJ55KMCS (linalool oxide); Q40Q9N063P (Acetic Acid); WM5Z385K7T (Pentylenetetrazole)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160914
[St] Status:MEDLINE


  10 / 642 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27206803
[Au] Autor:Driesschaert B; Bobko AA; Khramtsov VV; Zweier JL
[Ad] Endereço:In Vivo Multifunctional Magnetic Resonance center, Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, WV, 26506, USA. benoit.driesschaert@hsc.wvu.edu.
[Ti] Título:Nitro-Triarylmethyl Radical as Dual Oxygen and Superoxide Probe.
[So] Source:Cell Biochem Biophys;75(2):241-246, 2017 Jun.
[Is] ISSN:1559-0283
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Superoxide radical is involved in numerous physiological and pathophysiological processes. Tetrathiatriarylmethyl (TAM) radicals are known to react with superoxide allowing measurement of superoxide production in biological media. We report the synthesis of a Nitro conjugated TAM radical showing a rate constant of 7 × 10 M s which is two order of magnitude higher than other TAMs, allowing high sensitivity measurement of superoxide.
[Mh] Termos MeSH primário: Sondas Moleculares/química
Oxigênio/análise
Superóxidos/análise
Compostos de Tritil/química
[Mh] Termos MeSH secundário: Técnicas Eletroquímicas
Espectroscopia de Ressonância de Spin Eletrônica
Concentração de Íons de Hidrogênio
Sondas Moleculares/síntese química
Oxigênio/química
Soluções
Superóxidos/química
Compostos de Tritil/síntese química
Água/química
Xantina Oxidase/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Molecular Probes); 0 (Solutions); 0 (Trityl Compounds); 059QF0KO0R (Water); 11062-77-4 (Superoxides); EC 1.17.3.2 (Xanthine Oxidase); S88TT14065 (Oxygen)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160522
[St] Status:MEDLINE
[do] DOI:10.1007/s12013-016-0732-y



página 1 de 65 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde