Base de dados : MEDLINE
Pesquisa : D02.455.426.559.847.562.900.146 [Categoria DeCS]
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  1 / 1992 MEDLINE  
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[PMID]:29268103
[Au] Autor:Carusso S; Juárez AB; Moretton J; Magdaleno A
[Ad] Endereço:Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Salud Pública e Higiene Ambiental, Junin 956, 4° Piso, C1113 AAC, Buenos Aires, Argentina; Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental y Departa
[Ti] Título:Effects of three veterinary antibiotics and their binary mixtures on two green alga species.
[So] Source:Chemosphere;194:821-827, 2018 Mar.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The individual and combined toxicities of chlortetracycline (CTC), oxytetracycline (OTC) and enrofloxacin (ENF) have been examined in two green algae representative of the freshwater environment, the international standard strain Pseudokichneriella subcapitata and the native strain Ankistrodesmus fusiformis. The toxicities of the three antibiotics and their mixtures were similar in both strains, although low concentrations of ENF and CTC + ENF were more toxic in A. fusiformis than in the standard strain. The toxicological interactions of binary mixtures were predicted using the two classical models of additivity: Concentration Addition (CA) and Independent Action (IA), and compared to the experimentally determined toxicities over a range of concentrations between 0.1 and 10 mg L . The CA model predicted the inhibition of algal growth in the three mixtures in P. subcapitata, and in the CTC + OTC and CTC + ENF mixtures in A. fusiformis. However, this model underestimated the experimental results obtained in the OTC + ENF mixture in A. fusiformis. The IA model did not predict the experimental toxicological effects of the three mixtures in either strain. The sum of the toxic units (TU) for the mixtures was calculated. According to these values, the binary mixtures CTC + ENF and OTC + ENF showed an additive effect, and the CTC + OTC mixture showed antagonism in P. subcapitata, whereas the three mixtures showed synergistic effects in A. fusiformis. Although A. fusiformis was isolated from a polluted river, it showed a similar sensitivity with respect to P. subcapitata when it was exposed to binary mixtures of antibiotics.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Clorófitas/efeitos dos fármacos
Drogas Veterinárias/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/toxicidade
Clortetraciclina/toxicidade
Interações Medicamentosas
Fluoroquinolonas/toxicidade
Biologia de Ecossistemas de Água Doce
Oxitetraciclina/toxicidade
Drogas Veterinárias/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Fluoroquinolones); 0 (Veterinary Drugs); 3DX3XEK1BN (enrofloxacin); WCK1KIQ23Q (Chlortetracycline); X20I9EN955 (Oxytetracycline)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE


  2 / 1992 MEDLINE  
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[PMID]:27773254
[Au] Autor:Pulicharla R; Brar SK; Rouissi T; Auger S; Drogui P; Verma M; Surampalli RY
[Ad] Endereço:INRS-ETE, Université du Québec, 490, Rue de la Couronne, Québec G1K 9A9, Canada.
[Ti] Título:Degradation of chlortetracycline in wastewater sludge by ultrasonication, Fenton oxidation, and ferro-sonication.
[So] Source:Ultrason Sonochem;34:332-342, 2017 01.
[Is] ISSN:1873-2828
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Residual emerging contaminants in wastewater sludge remain an obstacle for its wide and safe applications such as landfilling and bio-fertilizer. In this study, the feasibility of individual ultrasonication (UlS) and Fenton oxidation (FO) and combined, Ferro-sonication processes (FO) on the degradation of chlortetracycline (CTC) in wastewater sludge was investigated. UlS parameters such as amplitude and sonication time were optimized by response surface methodology (RSM) for further optimization of FS process. Generation of highly reactive hydroxyl radicals in FO and FS processes were compared to evaluate the degradation efficiency of CTC. Increasing in the ratio of hydrogen peroxide and iron concentration showed increased CTC degradation in FO process; whereas in FS, an increase in iron concentration did not show any significant effect (p>0.05) on CTC degradation in sludge. The estimated iron concentration in sludge (115mg/kg) was enough to degrade CTC without the addition of external iron. The only adjustment of sludge pH to 3 was enough to generate in-situ hydroxyl radicals by utilizing iron which is already present in the sludge. This observation was further supported by hydroxyl radical estimation with adjustment of water pH to 3 and with and without the addition of iron. The optimum operating UlS conditions were found to be 60% amplitude for 106min by using RSM. Compared to standalone UlS and FO at 1:1 ratio, FS showed 15% and 8% increased CTC degradation respectively. In addition, UlS of sludge increased estrogenic activity 1.5 times higher compared to FO. FS treated samples did not show any estrogenic activity.
[Mh] Termos MeSH primário: Clortetraciclina/química
Clortetraciclina/isolamento & purificação
Peróxido de Hidrogênio/química
Ferro/química
Esgotos/química
Sonicação
Purificação da Água/métodos
[Mh] Termos MeSH secundário: Estrogênios/química
Estrogênios/isolamento & purificação
Oxirredução
Poluentes Químicos da Água/química
Poluentes Químicos da Água/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Estrogens); 0 (Fenton's reagent); 0 (Sewage); 0 (Water Pollutants, Chemical); BBX060AN9V (Hydrogen Peroxide); E1UOL152H7 (Iron); WCK1KIQ23Q (Chlortetracycline)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180131
[Lr] Data última revisão:
180131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  3 / 1992 MEDLINE  
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[PMID]:28462738
[Au] Autor:Volkova VV; Cazer CL; Gröhn YT
[Ad] Endereço:Department of Diagnostic Medicine/Pathobiology,Institute of Computational Comparative Medicine, College of Veterinary Medicine, Kansas State University,Mosier Hall, KS 66506,USA.
[Ti] Título:Models of antimicrobial pressure on intestinal bacteria of the treated host populations.
[So] Source:Epidemiol Infect;145(10):2081-2094, 2017 07.
[Is] ISSN:1469-4409
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Antimicrobial drugs are used to treat pathogenic bacterial infections in animals and humans. The by-stander enteric bacteria of the treated host's intestine can become exposed to the drug or its metabolites reaching the intestine in antimicrobially active form. We consider which processes and variables need to be accounted for to project the antimicrobial concentrations in the host's intestine. Those include: the drug's fraction (inclusive of any active metabolites) excreted in bile; the drug's fractions and intestinal segments of excretion via other mechanisms; the rates and intestinal segments of the drug's absorption and re-absorption; the rates and intestinal segments of the drug's abiotic and biotic degradation in the intestine; the digesta passage time through the intestinal segments; the rates, mechanisms, and reversibility of the drug's sorption to the digesta and enteric microbiome; and the volume of luminal contents in the intestinal segments. For certain antimicrobials, the antimicrobial activity can further depend on the aeration and chemical conditions in the intestine. Model forms that incorporate the inter-individual variation in those relevant variables can support projections of the intestinal antimicrobial concentrations in populations of treated host, such as food animals. To illustrate the proposed modeling framework, we develop two examples of treatments of bovine respiratory disease in beef steers by oral chlortetracycline and injectable third-generation cephalosporin ceftiofur. The host's diet influences the digesta passage time, volume, and digesta and microbiome composition, and may influence the antimicrobial loss due to degradation and sorption in the intestine. We consider two diet compositions in the illustrative simulations. The examples highlight the extent of current ignorance and need for empirical data on the variables influencing the selective pressures imposed by antimicrobial treatments on the host's intestinal bacteria.
[Mh] Termos MeSH primário: Complexo Respiratório Bovino/tratamento farmacológico
Cefalosporinas/administração & dosagem
Cefalosporinas/farmacologia
Clortetraciclina/administração & dosagem
Clortetraciclina/farmacologia
Microbioma Gastrointestinal/efeitos dos fármacos
Modelos Biológicos
[Mh] Termos MeSH secundário: Administração Oral
Animais
Anti-Infecciosos/administração & dosagem
Anti-Infecciosos/farmacologia
Complexo Respiratório Bovino/microbiologia
Injeções/veterinária
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Cephalosporins); 83JL932I1C (ceftiofur); WCK1KIQ23Q (Chlortetracycline)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1017/S095026881700084X


  4 / 1992 MEDLINE  
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[PMID]:28432950
[Au] Autor:Wang R; Chen M; Feng F; Zhang J; Sui Q; Tong J; Wei Y; Wei D
[Ad] Endereço:State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, PR China; Department of Water Pollution Control Technology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sc
[Ti] Título:Effects of chlortetracycline and copper on tetracyclines and copper resistance genes and microbial community during swine manure anaerobic digestion.
[So] Source:Bioresour Technol;238:57-69, 2017 Aug.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:As antibiotic and heavy metals are over used in the livestock industry, animal manure is a reservoir of antibiotic resistance genes (ARGs). Anaerobic digestion has been reported to have the potential to reduce ARGs. However, few studies investigated whether reduction of ARGs would be affected by different external pressures including antibiotics and heavy metals during anaerobic digestion. The purpose of this study was thus to investigate effects of both chlortetracycline (CTC) and Cu on reduction of ARGs, heavy metal resistance genes (HMRGs) and mobile genetic elements (MGEs) during the swine manure anaerobic digestion. The results showed that the predominant ARGs (tetO, tetW, tetX, tetL) could be effectively reduced (approximately 1.00 log copies/g TS) through mesophilic anaerobic digestion. Microbial community evolution was the main driver. It was interesting that Treponema might indicate the termination of anaerobic digestion and compete with ARGs host bacteria. Addition of CTC, Cu and CTC+Cu affected microbial community change and hindered removal of ARGs, especially, CTC+Cu seriously affected Treponema and ARGs during anaerobic digestion.
[Mh] Termos MeSH primário: Clortetraciclina
Cobre
Resistência Microbiana a Medicamentos
Esterco
Tetraciclinas
[Mh] Termos MeSH secundário: Animais
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Manure); 0 (Tetracyclines); 789U1901C5 (Copper); WCK1KIQ23Q (Chlortetracycline)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170423
[St] Status:MEDLINE


  5 / 1992 MEDLINE  
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[PMID]:28277084
[Au] Autor:Popova IE; Josue RDR; Deng S; Hattey JA
[Ad] Endereço:a Department of Plant , Soil, and Entomological Sciences, University of Idaho , Moscow , Idaho , USA.
[Ti] Título:Tetracycline resistance in semi-arid agricultural soils under long-term swine effluent application.
[So] Source:J Environ Sci Health B;52(5):298-305, 2017 May 04.
[Is] ISSN:1532-4109
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Annually, millions pounds of antibiotics are released unmetabolized into environment along with animal wastes. Accumulation of antibiotics in soils could potentially induce the persistence of antibiotic resistant bacteria. Antibiotics such as tetracyclines and tetracycline-resistant bacteria have been previously detected in fields fertilized with animal manure. However, little is known about the accumulation of tetracyclines and the development of tetracycline resistance in semi-arid soils. Here we demonstrate that continuous land application with swine effluent, containing trace amounts of chlortetracycline, does not necessarily induce tetracycline resistance in soil bacteria. Based on the testing of more than 3,000 bacteria isolated from the amended soils, we found no significant increase in the occurrence and level of chlortetracycline resistant bacteria in soils after 15 years of continuous swine effluent fertilization. To account for a possible transfer of tetracycline-resistant bacteria originated from the swine effluent to soils, we analyzed two commonly found tetracycline resistant genes, tet(O) and tet(M), in the swine effluent and fertilized soils. Both genes were present in the swine effluent, however, they were not detectable in soils applied with swine effluent. Our data demonstrate that agronomic application of manure from antibiotic treated swine effluent does not necessarily result in the development of antibiotic bacterial resistance in soils. Apparently, concentrations of chlortetracycline present in manure are not significant enough to induce the development of antibiotic bacterial resistance.
[Mh] Termos MeSH primário: Bactérias/efeitos dos fármacos
Esterco/microbiologia
Microbiologia do Solo
Resistência a Tetraciclina/efeitos dos fármacos
[Mh] Termos MeSH secundário: Agricultura
Animais
Antibacterianos/farmacologia
Clortetraciclina/análise
Clortetraciclina/farmacologia
Fertilizantes
Testes de Sensibilidade Microbiana
Oklahoma
Poluentes do Solo/análise
Suínos
Tetraciclina/farmacologia
Tetraciclinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Fertilizers); 0 (Manure); 0 (Soil Pollutants); 0 (Tetracyclines); F8VB5M810T (Tetracycline); WCK1KIQ23Q (Chlortetracycline)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.1080/03601234.2017.1281639


  6 / 1992 MEDLINE  
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[PMID]:28271383
[Au] Autor:Li W; Yao Z; Zhang X; Huang F; Lin W; Lin X
[Ad] Endereço:Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 35002, People's Republic of China.
[Ti] Título:Global protein expression profile response of planktonic Aeromonas hydrophila exposed to chlortetracycline.
[So] Source:World J Microbiol Biotechnol;33(4):68, 2017 Apr.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The antibiotics resistance phenomena of Aeromonas hydrophila has become serious economic and public health problems for the world aquaculture industry and human health care. In this study, to investigate the instinct antibiotics adaptive mechanism of this pathogen, iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) based quantitative proteomics technologies were performed to compare the differential expression of A. hydrophila in planktonic status in response to chlortetracycline (CTC) stress and then identified total 1552 proteins including 285 altered proteins with 90 increasing and 195 decreasing abundance proteins. The following bioinformatics analysis showed that many metabolic metabolism pathways such as carbon metabolism, pyruvate metabolism, and glycolysis/gluconeogenesis were trend to down-regulated whereas ß-Lactam resistance, RNA degradation, and amino acids biosynthesis processes were more likely to increase in CTC stress. The related pyruvate metabolism and ß-Lactam resistance processes in mRNA level were further measured using the q-PCR method. Thus, an understanding of the behaviors of A. hydrophila in response to CTC would be helpful to reveal the antibiotics adaptive mechanism and for the development of novel antibiotics therapy.
[Mh] Termos MeSH primário: Aeromonas hydrophila/metabolismo
Antibacterianos/farmacologia
Proteínas de Bactérias/metabolismo
Clortetraciclina/farmacologia
Proteômica/métodos
[Mh] Termos MeSH secundário: Aeromonas hydrophila/efeitos dos fármacos
Aeromonas hydrophila/genética
Proteínas de Bactérias/genética
Biologia Computacional/métodos
Farmacorresistência Bacteriana
Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos
Redes e Vias Metabólicas/efeitos dos fármacos
Plâncton/efeitos dos fármacos
Plâncton/genética
Plâncton/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Bacterial Proteins); WCK1KIQ23Q (Chlortetracycline)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-017-2204-y


  7 / 1992 MEDLINE  
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[PMID]:28128649
[Au] Autor:Hume ME; Donskey CJ
[Ad] Endereço:1 Food and Feed Safety Research Unit, Southern Plains Agricultural Research Center , Agricultural Research Service, U.S. Department of Agriculture, College Station, Texas.
[Ti] Título:Effect of Vancomycin, Tylosin, and Chlortetracycline on Vancomycin-Resistant Enterococcus faecium Colonization of Broiler Chickens During Grow-Out.
[So] Source:Foodborne Pathog Dis;14(4):231-237, 2017 Apr.
[Is] ISSN:1556-7125
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Broiler chickens may serve as reservoirs for human colonization by vancomycin-resistant Enterococcus (VRE). We examined the effects of vancomycin and two commonly used antimicrobial feed additives on VRE colonization in broiler chickens during grow-out. Chicks received unsupplemented feed or feed containing vancomycin, chlortetracycline, or tylosin from day of hatch to grow-out at 6 weeks. At 3 days of age, chicks received by crop gavage 10 colony-forming units (CFUs) of a human or poultry VRE isolate. Cecal contents were monitored weekly for VRE, short-chain fatty acids (SCFAs), and bacterial denaturing gradient gel electrophoresis (DGGE) profile methods. Vancomycin promoted persistent and high-level colonization with human- and poultry-derived VRE to grow-out in comparison with controls, while treatment with chlortetracycline and tylosin did not. Colonization by the poultry isolate in control, chlortetracycline, and tylosin groups persisted throughout the grow-out period with low concentrations present at 6 weeks, whereas the human isolate decreased to an undetectable level by week 6. Vancomycin resulted in significant reductions in cecal acetic acid and butyric acid in comparison with controls, but chlortetracycline and tylosin did not. DGGE profiles contained two main clusters with all vancomycin profiles in a smaller cluster and all other profiles in a larger cluster. These results demonstrate that vancomycin, but not chlortetracycline or tylosin, disrupted the indigenous microbiota and SCFA patterns of broiler chickens and promoted colonization by VRE.
[Mh] Termos MeSH primário: Galinhas/microbiologia
Clortetraciclina/farmacologia
Farmacorresistência Bacteriana Múltipla
Enterococcus faecium/efeitos dos fármacos
Tilosina/farmacologia
Vancomicina/farmacologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/farmacologia
Ceco/microbiologia
Contagem de Colônia Microbiana
Eletroforese em Gel de Gradiente Desnaturante
Enterococcus faecium/isolamento & purificação
Ácidos Graxos Voláteis/análise
Fezes/microbiologia
Contaminação de Alimentos/análise
Microbiologia de Alimentos
Aves Domésticas/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Fatty Acids, Volatile); 6Q205EH1VU (Vancomycin); WCK1KIQ23Q (Chlortetracycline); YEF4JXN031 (Tylosin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE
[do] DOI:10.1089/fpd.2016.2217


  8 / 1992 MEDLINE  
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[PMID]:28115143
[Au] Autor:Wang W; Tian G; Zong L; Zhou Y; Kang Y; Wang Q; Wang A
[Ad] Endereço:Center of Eco-materials and Green Chemistry, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China; Key Laboratory of Clay Mineral Applied Research of Gansu Province, Lanzhou 730000, China.
[Ti] Título:From illite/smectite clay to mesoporous silicate adsorbent for efficient removal of chlortetracycline from water.
[So] Source:J Environ Sci (China);51:31-43, 2017 Jan.
[Is] ISSN:1001-0742
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A series of mesoporous silicate adsorbents with superior adsorption performance for hazardous chlortetracycline (CTC) were sucessfully prepared via a facile one-pot hydrothermal reaction using low-cost illite/smectite (IS) clay, sodium silicate and magnesium sulfate as the starting materials. In this process, IS clay was "teared up" and then "rebuilt" as new porous silicate adsorbent with high specific surface area of 363.52m /g (about 8.7 folds higher than that of IS clay) and very negative Zeta potential (-34.5mV). The inert SiOSi (Mg, Al) bonds in crystal framework of IS were broken to form Si(Al) O groups with good adsorption activity, which greatly increased the adsorption sites served for holding much CTC molecules. Systematic evaluation on adsorption properties reveals the optimal silicate adsorbent can adsorb 408.81mg/g of CTC (only 159.7mg/g for raw IS clay) and remove 99.3% (only 46.5% for raw IS clay) of CTC from 100mg/L initial solution (pH3.51; adsorption temperature 30°C; adsorbent dosage, 3g/L). The adsorption behaviors of CTC onto the adsorbent follows the Langmuir isotherm model, Temkin equation and pseudo second-order kinetic model. The mesopore adsorption, electrostatic attraction and chemical association mainly contribute to the enhanced adsorption properties. As a whole, the high-efficient silicate adsorbent could be candidates to remove CTC from the wastewater with high amounts of CTC.
[Mh] Termos MeSH primário: Silicatos de Alumínio/química
Antibacterianos/química
Clortetraciclina/química
Poluentes Químicos da Água/química
[Mh] Termos MeSH secundário: Adsorção
Antibacterianos/análise
Clortetraciclina/análise
Cinética
Minerais/química
Modelos Químicos
Silicatos/química
Temperatura Ambiente
Águas Residuais/química
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aluminum Silicates); 0 (Anti-Bacterial Agents); 0 (Minerals); 0 (Silicates); 0 (Waste Water); 0 (Water Pollutants, Chemical); 12173-60-3 (illite); 1302-87-0 (clay); A3N5ZCN45C (Smectite); IJF18F77L3 (sodium silicate); WCK1KIQ23Q (Chlortetracycline)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170209
[Lr] Data última revisão:
170209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE


  9 / 1992 MEDLINE  
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[PMID]:27805474
[Au] Autor:Long C; Deng B; Sun S; Meng S
[Ad] Endereço:a Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), School of Chemistry and Pharmaceutical Sciences , Guangxi Normal University , Guilin , China.
[Ti] Título:Simultaneous determination of chlortetracycline, ampicillin and sarafloxacin in milk using capillary electrophoresis with electrochemiluminescence detection.
[So] Source:Food Addit Contam Part A Chem Anal Control Expo Risk Assess;34(1):24-31, 2017 Jan.
[Is] ISSN:1944-0057
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A fast, inexpensive and sensitive approach for the simultaneous determination of chlortetracycline, ampicilline and sarafloxacin in milk was developed using capillary electrophoresis coupled with an electrochemiluminescence detector. Under the optimal detection conditions, the linear ranges for chlortetracyline, ampicilline and sarafloxacin were 0.030-5.0, 0.050-5.0 and 0.0040-2.0 µg ml , respectively. The correlation coefficients of chlortetracycline, ampicilline and sarafloxacin were determined as 0.9997, 0.9952 and 0.9978, respectively. Detection limits (S/N = 3) of chlortetracycline, ampicilline and sarafloxacin were found as 0.017, 0.018 and 0.0013 µg ml , respectively. This method was successfully applied for the determination of chlortetracycline, ampicilline and sarafloxacin in milk. The recoveries were between 95.3% and 100%. The relative standard deviations of the detection limit and recovery were less than 2.6% and 3.2%, respectively.
[Mh] Termos MeSH primário: Ampicilina/análise
Clortetraciclina/análise
Ciprofloxacino/análogos & derivados
Eletroforese Capilar/métodos
Leite/química
[Mh] Termos MeSH secundário: Animais
Ciprofloxacino/análise
Técnicas Eletroquímicas/métodos
Limite de Detecção
Medições Luminescentes
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
5E8K9I0O4U (Ciprofloxacin); 7C782967RD (Ampicillin); RC3WJ907XY (sarafloxacin); WCK1KIQ23Q (Chlortetracycline)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161103
[St] Status:MEDLINE
[do] DOI:10.1080/19440049.2016.1254820


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[PMID]:27757509
[Au] Autor:Zhang D; Ji H; Liu H; Wang S; Wang J; Wang Y
[Ad] Endereço:Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Shu Guang Hua Yuan Middle Road No. 9, Haidian District, Beijing, China.
[Ti] Título:Changes in the diversity and composition of gut microbiota of weaned piglets after oral administration of Lactobacillus or an antibiotic.
[So] Source:Appl Microbiol Biotechnol;100(23):10081-10093, 2016 Dec.
[Is] ISSN:1432-0614
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The gut microbiota plays important roles in the health and well-being of animals, and high-throughput sequencing facilitates exploration of microbial populations in the animal gut. However, previous studies have focused on fecal samples instead of the gastrointestinal tract. In this study, we compared the microbiota diversity and composition of intestinal contents of weaned piglets treated with Lactobacillus reuteri or chlortetracycline (aureomycin) using high-throughput sequencing. Nine weaned piglets were randomly divided into three groups and supplemented with L. reuteri, chlortetracycline, or saline for 10 days, and then the contents of three intestinal segments (jejunum, colon, and cecum) were obtained and used for sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. The microbiota diversity and composition in the jejunum were different from those in the colon and cecum among the three treatments. In the jejunum, treatment with L. reuteri increased the species richness of the microbiota, as indicated by the ACE and Chao1 indexes, compared with the chlortetracycline group, in which several taxa were eliminated. In the colon and cecum, relative abundances of the phylum Firmicutes and the genus Prevotella were higher in the chlortetracycline group than in the other groups. Distances between clustered samples revealed that the L. reuteri group was closer to the chlortetracycline group than the control group for jejunum samples, while colon and cecum samples of the L. reuteri group were clustered with those of the control group. This study provides fundamental knowledge for future studies such as the development of alternatives to antibiotics.
[Mh] Termos MeSH primário: Antibacterianos/administração & dosagem
Bactérias/classificação
Microbioma Gastrointestinal/efeitos dos fármacos
Lactobacillus reuteri/crescimento & desenvolvimento
Probióticos/administração & dosagem
[Mh] Termos MeSH secundário: Administração Oral
Animais
Animais Recém-Nascidos
Bactérias/efeitos dos fármacos
Bactérias/genética
Ceco/microbiologia
Clortetraciclina/administração & dosagem
Análise por Conglomerados
Colo/microbiologia
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Jejuno/microbiologia
Filogenia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); WCK1KIQ23Q (Chlortetracycline)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170124
[Lr] Data última revisão:
170124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE



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