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[PMID]:28471002
[Au] Autor:Cecchini MM; Reale S; Manini P; d'Ischia M; De Angelis F
[Ad] Endereço:Department of Physical and Chemical Sciences, University of L'Aquila, Via Vetoio, Coppito, L'Aquila, Italy.
[Ti] Título:Modeling Fungal Melanin Buildup: Biomimetic Polymerization of 1,8-Dihydroxynaphthalene Mapped by Mass Spectrometry.
[So] Source:Chemistry;23(33):8092-8098, 2017 Jun 12.
[Is] ISSN:1521-3765
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Due to the emerging biomedical relevance and technological potential of fungal melanins, and prompted by the virtual lack of information about their structural arrangement, an optimized synthetic protocol has been devised for a potential structural model of Ascomyces allomelanin through enzyme-catalyzed oxidative polymerization of 1,8-dihydroxynaphthalene (1,8-DHN). Electrospray ionization mass spectrometry (ESI-MS) measurements of freshly synthesized DHN-polymer recorded in the negative ion mode allowed detection of oligomers up to m/z 4000, separated by 158 Da, corresponding to the in-chain DHN-unit. The dominant peaks were assigned to singly-charged distribution, up to 23 repeating units, whereas a doubly charged polymer distribution was also detectable. Chemical derivatization, ultra-performance liquid chromatography (UPLC)-ESI MS, and MS/MS data confirmed that oxidative polymerization of 1,8-DHN proceeds through C-C coupling of the naphthalene rings. The new insights reported here into synthetic 1,8-DHN oligomers/polymers as a mimic of fungal melanins may guide novel interesting advances and applications in the field of biomimetic functional materials.
[Mh] Termos MeSH primário: Materiais Biomiméticos/química
Proteínas Fúngicas/metabolismo
Fungos/metabolismo
Melaninas/metabolismo
Naftóis/química
[Mh] Termos MeSH secundário: Biocatálise
Materiais Biomiméticos/metabolismo
Cromatografia Líquida de Alta Pressão
Proteínas Fúngicas/química
Peroxidase do Rábano Silvestre/metabolismo
Melaninas/química
Oxirredução
Polimerização
Espectrometria de Massas por Ionização por Electrospray
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungal Proteins); 0 (Melanins); 0 (Naphthols); 569-42-6 (1,8-dihydroxynaphthalene); EC 1.11.1.- (Horseradish Peroxidase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1002/chem.201701951


  2 / 3410 MEDLINE  
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[PMID]:28463421
[Au] Autor:Wu C; Ichinose K; Choi YH; van Wezel GP
[Ad] Endereço:Molecular Biotechnology, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE, Leiden, The Netherlands.
[Ti] Título:Aromatic Polyketide GTRI-02 is a Previously Unidentified Product of the act Gene Cluster in Streptomyces coelicolor A3(2).
[So] Source:Chembiochem;18(14):1428-1434, 2017 07 18.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The biosynthesis of aromatic polyketides derived from type II polyketide synthases (PKSs) is complex, and it is not uncommon that highly similar gene clusters give rise to diverse structural architectures. The act biosynthetic gene cluster (BGC) of the model actinomycete Streptomyces coelicolor A3(2) is an archetypal type II PKS. Here we show that the act BGC also specifies the aromatic polyketide GTRI-02 (1) and propose a mechanism for the biogenesis of its 3,4-dihydronaphthalen-1(2H)-one backbone. Polyketide 1 was also produced by Streptomyces sp. MBT76 after activation of the act-like qin gene cluster by overexpression of the pathway-specific activator. Mining of this strain also identified dehydroxy-GTRI-02 (2), which most likely originated from dehydration of 1 during the isolation process. This work shows that even extensively studied model gene clusters such as act of S. coelicolor can still produce new chemistry, offering new perspectives for drug discovery.
[Mh] Termos MeSH primário: Família Multigênica
Naftóis/metabolismo
Streptomyces coelicolor/genética
Streptomyces coelicolor/metabolismo
Tetra-Hidronaftalenos/metabolismo
[Mh] Termos MeSH secundário: Naftóis/química
Tetra-Hidronaftalenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (GTRI 02); 0 (Naphthols); 0 (Tetrahydronaphthalenes)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180122
[Lr] Data última revisão:
180122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201700107


  3 / 3410 MEDLINE  
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[PMID]:28873610
[Au] Autor:Piatkowska M; Jedziniak P; Olejnik M; Zmudzki J; Posyniak A
[Ad] Endereço:Department of Pharmacology and Toxicology, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland. Electronic address: marta.piatkowska@piwet.pulawy.pl.
[Ti] Título:Absence of evidence or evidence of absence? A transfer and depletion study of Sudan I in eggs.
[So] Source:Food Chem;239:598-602, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sudan I is a carcinogenic industrial azo-dye, forbidden for use in food. However, it has been detected in food on several occasions, such as in paprika, used in animal husbandry to enhance egg yolk colour. Therefore, an animal experiment was designed to simulate the transfer of Sudan I to eggs after its unintentional administration to laying hens. A group of laying hens (n=18) received feed contaminated with Sudan I at the raising concentrations: 0.45mg/kg, 4.97mg/kg and 42.1mg/kg. Residues of Sudan I were detected in egg yolks (0.29±0.03µg/kg, mean±SD) only after the administration of the feed contaminated with the dye at the highest concentration. The determined concentrations were much lower than expected based on the compound's lipophilicity. In conclusion, the transfer of Sudan I to eggs was limited and strongly dependent on its concentration in feed.
[Mh] Termos MeSH primário: Ovos
[Mh] Termos MeSH secundário: Ração Animal
Animais
Galinhas
Feminino
Naftóis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Naphthols); 48I7IBB68J (1-phenylazo-2-naphthol)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


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[PMID]:28981656
[Au] Autor:Wallingford AK; Cha DH; Linn CE; Wolfin MS; Loeb GM
[Ad] Endereço:Department of Entomology, Cornell University, 630 W. North St., Geneva, NY 14456.
[Ti] Título:Robust Manipulations of Pest Insect Behavior Using Repellents and Practical Application for Integrated Pest Management.
[So] Source:Environ Entomol;46(5):1041-1050, 2017 10 01.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In agricultural settings, examples of effective control strategies using repellent chemicals in integrated pest management (IPM) are relatively scarce compared to those using attractants. This may be partly due to a poor understanding of how repellents affect insect behavior once they are deployed. Here we attempt to identify potential hallmarks of repellent stimuli that are robust enough for practical use in the field. We explore the literature for success stories using repellents in IPM and we investigate the mechanisms of repellency for two chemical oviposition deterrents for controlling Drosophila suzukii Matsumura, a serious pest of small fruit crops. Drosophila suzukii causes injury by laying her eggs in ripening fruit and resulting larvae make fruit unmarketable. In caged choice tests, reduced oviposition was observed in red raspberry fruit treated with volatile 1-octen-3-ol and geosmin at two initial concentrations (10% and 1%) compared to untreated controls. We used video monitoring to observe fly behavior in these caged choice tests and investigate the mode of action for deterrence through the entire behavioral repertoire leading to oviposition. We observed fewer visitors and more time elapsed before flies first landed on 1-octen-3-ol-treated fruits than control fruits and concluded that this odor primarily inhibits behaviors that occur before D. suzukii comes in contact with a potential oviposition substrate (precontact). We observed some qualitative differences in precontact behavior of flies around geosmin-treated fruits; however, we concluded that this odor primarily inhibits behaviors that occur after D. suzukii comes in contact with treated fruits (postcontact). Field trials found reduced oviposition in red raspberry treated with 1-octen-3-ol and a combination of 1-octen-3-ol and geosmin, but no effect of geosmin alone. Recommendations for further study of repellents for practical use in the field are discussed.
[Mh] Termos MeSH primário: Controle de Insetos/métodos
Repelentes de Insetos/farmacologia
Naftóis/farmacologia
Octanóis/farmacologia
Oviposição/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Drosophila
Feminino
Rubus
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Insect Repellents); 0 (Naphthols); 0 (Octanols); MYW912WXJ4 (geosmin); WXB511GE38 (1-octen-3-ol)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171006
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvx125


  5 / 3410 MEDLINE  
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[PMID]:28858527
[Au] Autor:Benabdi S; Peurois F; Nawrotek A; Chikireddy J; Cañeque T; Yamori T; Shiina I; Ohashi Y; Dan S; Rodriguez R; Cherfils J; Zeghouf M
[Ad] Endereço:Laboratoire de Biologie et Pharmacologie Appliquée CNRS, Ecole Normale Supérieure Paris-Saclay , 61 avenue du président Wilson, 94235 Cachan, France.
[Ti] Título:Family-wide Analysis of the Inhibition of Arf Guanine Nucleotide Exchange Factors with Small Molecules: Evidence of Unique Inhibitory Profiles.
[So] Source:Biochemistry;56(38):5125-5133, 2017 Sep 26.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arf GTPases and their guanine nucleotide exchange factors (ArfGEFs) are major regulators of membrane traffic and organelle structure in cells. They are associated with a variety of diseases and are thus attractive therapeutic targets for inhibition by small molecules. Several inhibitors of unrelated chemical structures have been discovered, which have shown their potential in dissecting molecular pathways and blocking disease-related functions. However, their specificity across the ArfGEF family has remained elusive. Importantly, inhibitory responses in the context of membranes, which are critical determinants of Arf and ArfGEF cellular functions, have not been investigated. Here, we compare the efficiency and specificity of four structurally distinct ArfGEF inhibitors, Brefeldin A, SecinH3, M-COPA, and NAV-2729, toward six ArfGEFs (human ARNO, EFA6, BIG1, and BRAG2 and Legionella and Rickettsia RalF). Inhibition was assessed by fluorescence kinetics using pure proteins, and its modulation by membranes was determined with lipidated GTPases in the presence of liposomes. Our analysis shows that despite the intra-ArfGEF family resemblance, each inhibitor has a specific inhibitory profile. Notably, M-COPA is a potent pan-ArfGEF inhibitor, and NAV-2729 inhibits all GEFs, the strongest effects being against BRAG2 and Arf1. Furthermore, the presence of the membrane-binding domain in Legionella RalF reveals a strong inhibitory effect of BFA that is not measured on its GEF domain alone. This study demonstrates the value of family-wide assays with incorporation of membranes, and it should enable accurate dissection of Arf pathways by these inhibitors to best guide their use and development as therapeutic agents.
[Mh] Termos MeSH primário: Brefeldina A/farmacologia
Fatores de Troca do Nucleotídeo Guanina/antagonistas & inibidores
Naftóis/farmacologia
Pirazóis/farmacologia
Piridinas/farmacologia
Pirimidinonas/farmacologia
Triazóis/farmacologia
[Mh] Termos MeSH secundário: Fatores de Ribosilação do ADP/antagonistas & inibidores
Fatores de Ribosilação do ADP/genética
Fatores de Ribosilação do ADP/metabolismo
Membrana Celular
Fluorescência
Proteínas Ativadoras de GTPase/antagonistas & inibidores
Fatores de Troca do Nucleotídeo Guanina/genética
Fatores de Troca do Nucleotídeo Guanina/metabolismo
Seres Humanos
Lipossomos/química
Soluções
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (GTPase-Activating Proteins); 0 (Guanine Nucleotide Exchange Factors); 0 (IQSEC1 protein, human); 0 (Liposomes); 0 (N-(pyridine-3-ylmethyl)-5-(7-hydroxy-2,6,8-trimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalene-1-yl)-2-methylpenta-2,4-dienamide); 0 (NAV-2729); 0 (Naphthols); 0 (Pyrazoles); 0 (Pyridines); 0 (Pyrimidinones); 0 (RalF protein, Legionella pneumophila); 0 (SecinH3); 0 (Solutions); 0 (Triazoles); 0 (cytohesin-2); 20350-15-6 (Brefeldin A); EC 3.6.5.2 (ADP-Ribosylation Factors); EC 3.6.5.2 (ADP-ribosylation factor 6)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00706


  6 / 3410 MEDLINE  
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[PMID]:28842491
[Au] Autor:Bhat S; Hasenhuetl PS; Kasture A; El-Kasaby A; Baumann MH; Blough BE; Sucic S; Sandtner W; Freissmuth M
[Ad] Endereço:From the Institute of Pharmacology and the Gaston H. Glock Research Laboratories for Exploratory Drug Development, Center of Physiology and Pharmacology, Medical University of Vienna, A-1090 Vienna, Austria.
[Ti] Título:Conformational state interactions provide clues to the pharmacochaperone potential of serotonin transporter partial substrates.
[So] Source:J Biol Chem;292(40):16773-16786, 2017 Oct 06.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Point mutations in SLC6 transporters cause misfolding, which can be remedied by pharmacochaperones. The serotonin transporter (SERT/SLC6A4) has a rich pharmacology including inhibitors, releasers (amphetamines, which promote the exchange mode), and more recently, discovered partial substrates. We hypothesized that partial substrates trapped the transporter in one or several states of the transport cycle. This conformational trapping may also be conducive to folding. We selected naphthylpropane-2-amines of the phenethylamine library (PAL) including the partial substrate PAL1045 and its congeners PAL287 and PAL1046. We analyzed their impact on the transport cycle of SERT by biochemical approaches and by electrophysiological recordings; substrate-induced peak currents and steady-state currents monitored the translocation of substrate and co-substrate Na across the lipid bilayer and the transport cycle, respectively. These experiments showed that PAL1045 and its congeners bound with different affinities (ranging from nm to µm) to various conformational intermediates of SERT during the transport cycle. Consistent with the working hypothesis, PAL1045 was the most efficacious compound in restoring surface expression and transport activity to the folding-deficient mutant SERT- PG -AA. These experiments provide a proof-of-principle for a rational search for pharmacochaperones, which may be useful to restore function to clinically relevant folding-deficient transporter mutants.
[Mh] Termos MeSH primário: Chaperonas Moleculares/química
Naftóis/química
Proteínas da Membrana Plasmática de Transporte de Serotonina/química
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Células HEK293
Seres Humanos
Transporte de Íons
Bicamadas Lipídicas/química
Chaperonas Moleculares/farmacologia
Mutação de Sentido Incorreto
Naftóis/farmacologia
Conformação Proteica
Dobramento de Proteína
Proteínas da Membrana Plasmática de Transporte de Serotonina/genética
Proteínas da Membrana Plasmática de Transporte de Serotonina/metabolismo
Sódio/química
Sódio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipid Bilayers); 0 (Molecular Chaperones); 0 (Naphthols); 0 (SLC6A4 protein, human); 0 (Serotonin Plasma Membrane Transport Proteins); 9NEZ333N27 (Sodium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170827
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.794081


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[PMID]:28793307
[Au] Autor:Bashyal B; Li L; Bains T; Debnath A; LaBarbera DV
[Ad] Endereço:Department of Pharmaceutical Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, Colorado, United States of America.
[Ti] Título:Larrea tridentata: A novel source for anti-parasitic agents active against Entamoeba histolytica, Giardia lamblia and Naegleria fowleri.
[So] Source:PLoS Negl Trop Dis;11(8):e0005832, 2017 Aug.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Protozoan parasites infect and kill millions of people worldwide every year, particularly in developing countries where access to clean fresh water is limited. Among the most common are intestinal parasites, including Giardia lamblia and Entamoeba histolytica. These parasites wreak havoc on the epithelium lining the small intestines (G. lamblia) and colon (E. histolytica) causing giardiasis and amebiasis, respectively. In addition, there are less common but far more deadly pathogens such as Naegleria fowleri that thrive in warm waters and infect the central nervous systems of their victims via the nasal passages. Despite their prevalence and associated high mortality rates, there remains an unmet need to identify more effective therapeutics for people infected with these opportunistic parasites. To address this unmet need, we have surveyed plants and traditional herbal medicines known throughout the world to identify novel antiparasitic agents with activity against G. lamblia, E. histolytica, and N. fowleri. Herein, we report Larrea tridentata, known as creosote bush, as a novel source for secondary metabolites that display antiparasitic activity against all three pathogens. This report also characterizes the lignan compound classes, nordihydroguairetic acid and demethoxyisoguaiacin, as novel antiparasitic lead agents to further develop more effective drug therapy options for millions of people worldwide.
[Mh] Termos MeSH primário: Antiprotozoários/farmacologia
Entamoeba histolytica/efeitos dos fármacos
Giardia lamblia/efeitos dos fármacos
Larrea/química
Naegleria fowleri/efeitos dos fármacos
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antiprotozoários/química
Antiprotozoários/isolamento & purificação
Seres Humanos
Masoprocol/isolamento & purificação
Masoprocol/farmacologia
Naftóis/isolamento & purificação
Naftóis/farmacologia
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (Naphthols); 0 (Plant Extracts); 0 (demethoxyisoguaiacin); 7BO8G1BYQU (Masoprocol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005832


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[PMID]:28792943
[Au] Autor:Talbot JJ; Houbraken J; Frisvad JC; Samson RA; Kidd SE; Pitt J; Lindsay S; Beatty JA; Barrs VR
[Ad] Endereço:Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camperdown, New South Wales, Australia.
[Ti] Título:Discovery of Aspergillus frankstonensis sp. nov. during environmental sampling for animal and human fungal pathogens.
[So] Source:PLoS One;12(8):e0181660, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Invasive fungal infections (IFI) due to species in Aspergillus section Fumigati (ASF), including the Aspergillus viridinutans species complex (AVSC), are increasingly reported in humans and cats. The risk of exposure to these medically important fungi in Australia is unknown. Air and soil was sampled from the domiciles of pet cats diagnosed with these IFI and from a nature reserve in Frankston, Victoria, where Aspergillus viridinutans sensu stricto was discovered in 1954. Of 104 ASF species isolated, 61% were A. fumigatus sensu stricto, 9% were AVSC (A. felis-clade and A. frankstonensis sp. nov.) and 30% were other species (30%). Seven pathogenic ASF species known to cause disease in humans and animals (A. felis-clade, A. fischeri, A. thermomutatus, A. lentulus, A. laciniosus A. fumisynnematus, A. hiratsukae) comprised 25% of isolates overall. AVSC species were only isolated from Frankston soil where they were abundant, suggesting a particular ecological niche. Phylogenetic, morphological and metabolomic analyses of these isolates identified a new species, A. frankstonensis that is phylogenetically distinct from other AVSC species, heterothallic and produces a unique array of extrolites, including the UV spectrum characterized compounds DOLD, RAIMO and CALBO. Shared morphological and physiological characteristics with other AVSC species include slow sporulation, optimal growth at 37°C, no growth at 50°C, and viriditoxin production. Overall, the risk of environmental exposure to pathogenic species in ASF in Australia appears to be high, but there was no evidence of direct environmental exposure to AVSC species in areas where humans and cats cohabitate.
[Mh] Termos MeSH primário: Aspergilose/epidemiologia
Aspergillus/classificação
Aspergillus/isolamento & purificação
Monitoramento Ambiental/métodos
Infecções Fúngicas Invasivas/epidemiologia
[Mh] Termos MeSH secundário: Animais
Antifúngicos/farmacologia
Aspergilose/microbiologia
Aspergillus/efeitos dos fármacos
Aspergillus/genética
Austrália/epidemiologia
Sequência de Bases
Gatos
DNA Fúngico/genética
Exposição Ambiental
Seres Humanos
Infecções Fúngicas Invasivas/microbiologia
Testes de Sensibilidade Microbiana
Naftóis/metabolismo
Filogenia
Análise de Sequência de DNA
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (DNA, Fungal); 0 (Naphthols); 35483-50-2 (viriditoxin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181660


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[PMID]:28764093
[Au] Autor:Li Y; Wang A; Bai Y; Wang S
[Ad] Endereço:College of Food Science and Nutritional Engineering, China Agricultural University, 17# Qinghua East Road, Haidian District, Beijing 100083, China. Electronic address: ying_li1992@163.com.
[Ti] Título:Acriflavine-immobilized eggshell membrane as a new solid-state biosensor for Sudan I-IV detection based on fluorescence resonance energy transfer.
[So] Source:Food Chem;237:966-973, 2017 Dec 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel solid-surface fluorescence biosensor for rapid detection of Sudan I-IV was proposed based on fluorescence resonance energy transfer (FRET). The biosensor was fabricated by immobilizing acriflavine (AY) on the eggshell membrane (ESM) with glutaraldehyde as cross-linking agent. FRET mechanism was demonstrated by using AY and Sudan dyes as donor and acceptor respectively, an efficient energy transfer in the present system was indicated by the sufficient spectral overlap integral (J) and proper Förster critical distance (R ). Under optimum conditions, the fluorescence of the AY-ESM could be efficiently quenched by Sudan I-IV and the corresponding linear range was 0.5-60µM with the detection limits (3σ/slope) of 0.16, 0.26, 0.21 and 0.17µM respectively. Compared to the detection of Sudan dyes in solution-state, the membrane biosensor exhibited advantages of low detection limits, high sensitivity and selectivity, as well as excellent stability. Recovery tests in spiked real samples also achieved satisfactory results.
[Mh] Termos MeSH primário: Compostos Azo/análise
Casca de Ovo
Naftóis/análise
[Mh] Termos MeSH secundário: Acriflavina
Animais
Técnicas Biossensoriais
Galinhas
Transferência Ressonante de Energia de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azo Compounds); 0 (Naphthols); 1T3A50395T (Acriflavine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE


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[PMID]:28728845
[Au] Autor:Park AK; Kim H; Kim IS; Roh SJ; Shin SC; Lee JH; Park H; Kim HW
[Ad] Endereço:Unit of Polar Genomics, Korea Polar Research Institute, Incheon 21990, South Korea.
[Ti] Título:Crystal structure of cis-dihydrodiol naphthalene dehydrogenase (NahB) from Pseudomonas sp. MC1: Insights into the early binding process of the substrate.
[So] Source:Biochem Biophys Res Commun;491(2):403-408, 2017 Sep 16.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The bacterial strain Pseudomonas sp. MC1 harbors an 81-kb metabolic plasmid, which encodes enzymes involved in the conversion of naphthalene to salicylate. Of these, the enzyme NahB (cis-dihydrodiol naphthalene dehydrogenase), which catalyzes the second reaction of this pathway, binds to various substrates such as cis-1,2-dihydro-1,2-dihydroxy-naphthalene (1,2-DDN), cis-2,3-dihydro-2,3-dihydroxybiphenyl (2,3-DDB), and 3,4-dihydro-3,4-dihydroxy-2,2',5,5'-tetrachlorobiphenyl (3,4-DD-2,2',5-5-TCB). However, the mechanism underlying its broad substrate specificity is unclear owing to the lack of structural information. Here, we determined the first crystal structures of NahB in the absence and presence of NAD and 2,3-dihydroxybiphenyl (2,3-DB). Structure analysis suggests that the flexible substrate-binding loop allows NahB to accommodate diverse substrates. Furthermore, we defined the initial steps of substrate recognition and identified the early substrate-binding site in the substrate recognition process through the complex structure with ligands.
[Mh] Termos MeSH primário: Compostos de Bifenilo/química
Catecóis/química
Naftóis/química
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/química
Bifenilos Policlorados/química
Pseudomonas/química
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Sítios de Ligação
Compostos de Bifenilo/metabolismo
Catecóis/metabolismo
Clonagem Molecular
Cristalografia por Raios X
Escherichia coli/genética
Escherichia coli/metabolismo
Expressão Gênica
Modelos Moleculares
Naftóis/metabolismo
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/genética
Oxirredutases atuantes sobre Doadores de Grupo CH-CH/metabolismo
Bifenilos Policlorados/metabolismo
Ligação Proteica
Conformação Proteica em alfa-Hélice
Conformação Proteica em Folha beta
Domínios e Motivos de Interação entre Proteínas
Pseudomonas/enzimologia
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biphenyl Compounds); 0 (Catechols); 0 (Naphthols); 0 (Recombinant Proteins); 1133-63-7 (2,3-dihydroxybiphenyl); 2R5017T335 (1,2-dihydroxynaphthalene); DFC2HB4I0K (Polychlorinated Biphenyls); EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors); EC 1.3.1.29 (cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170722
[St] Status:MEDLINE



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