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[PMID]:28844711
[Au] Autor:Fallatah MM; Liu S; Sevigny MB; Zou H; Louie MC
[Ad] Endereço:Department of Natural Sciences and Mathematics, Dominican University of California, 50 Acacia Avenue, San Rafael, CA 94901, USA.
[Ti] Título:Novel flexible heteroarotinoid, SL-1-18, promotes ERα degradation to inhibit breast cancer cell growth.
[So] Source:Cancer Lett;408:82-91, 2017 Nov 01.
[Is] ISSN:1872-7980
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:SL-1-18 (1-(chrysen-6-yl)-3-(4-nitrophenyl)thiourea) is new flexible heteroarotinoid (Flex-Het) analog derived from the parent compound, SHetA2, and our previous study showed comparable activity to SHetA2 in terms of inhibiting ER+ breast cancer cell growth. This current study aims to determine the molecular mechanism underlying SL-1-18's effect on breast cancer cell growth. Our results indicate that SL-1-18 inhibits cell proliferation of ER+ breast cancer cells (MCF-7 and T-47D) by preventing cell cycle progression. SL-1-18 treatment correlated positively with decreased expression of key cell-cycle regulators, such as cyclin D1, as well as other ERα-target genes at both the transcript and protein levels. Interestingly, decreased expression of ERα was also observed, with a significant reduction at the protein level within 2 h of SL-1-18 treatment, while the decrease in mRNA occurred at a later time point. ERα degradation was shown to be mediated by the ubiquitination-proteasome pathway. In summary, this is the first study to show that a Flex-Het- SL-1-18- can promote the degradation of ERα via the ubiquitin-proteasome pathway and should be further developed as a therapeutic option for ER+ breast cancer.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Neoplasias da Mama/tratamento farmacológico
Cromanos/farmacologia
Crisenos/farmacologia
Receptor alfa de Estrogênio/metabolismo
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Proteólise/efeitos dos fármacos
Tioureia/análogos & derivados
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Neoplasias da Mama/metabolismo
Neoplasias da Mama/patologia
Ciclo Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Feminino
Seres Humanos
Complexo de Endopeptidases do Proteassoma
Tionas/farmacologia
Tioureia/farmacologia
Células Tumorais Cultivadas
Ubiquitinação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 ((((4-nitrophenyl)amino)(2,2,4,4-tetramethyl thiochroman-6-yl)amino) methane-1-thione); 0 (1-(chrysen-6-yl)-3-(4-nitrophenyl)thiourea); 0 (Antineoplastic Agents); 0 (Chromans); 0 (Chrysenes); 0 (Estrogen Receptor alpha); 0 (Thiones); 0 (estrogen receptor alpha, human); EC 3.4.25.1 (Proteasome Endopeptidase Complex); GYV9AM2QAG (Thiourea)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE


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[PMID]:28601011
[Au] Autor:Diamante G; do Amaral E Silva Müller G; Menjivar-Cervantes N; Xu EG; Volz DC; Dias Bainy AC; Schlenk D
[Ad] Endereço:Department of Environmental Sciences, University of California, 900 University Ave., Riverside, CA 92521, USA.
[Ti] Título:Developmental toxicity of hydroxylated chrysene metabolites in zebrafish embryos.
[So] Source:Aquat Toxicol;189:77-86, 2017 Aug.
[Is] ISSN:1879-1514
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:One of the primary sources of polycyclic aromatic hydrocarbons (PAHs) in marine environments is oil. Photochemical oxidation and microbial transformation of PAH-containing oils can result in the formation of oxygenated products. Among the PAHs in crude oil, chrysene is one of the most persistent within the water column and may be transformed to 2- and 6-hydroxychrysene (OHCHR). Both of these compounds have been shown to activate (2-OHCHR) and antagonize (6-OHCHR) the estrogen receptor (ER). Previous studies in our lab have shown that estrogen can significantly alter zebrafish development. However, little is known about the developmental toxicity of hydroxylated PAHs. Zebrafish embryos were exposed to 0.5-10µM of 2- or 6-OHCHR from 2h post-fertilization (hpf) until 76hpf. A significant decrease in survival was observed following exposure to 6-OHCHR - but not 2-OHCHR. Both OHCHRs significantly increased the percentage of overall deformities after treatment. In addition to cardiac malformations, ocular and circulatory defects were also observed in embryos exposed to both compounds, while 2-OHCHR generally resulted in a higher prevalence of effect. Moreover, treatment with 2-OHCHR resulted in a significant decrease in hemoglobin levels. ER nor G-Protein coupled estrogen receptor (GPER) antagonists and agonists did not rescue the observed defects. We also analyzed the expression of cardiac-, eye- and circulation-related genes previously shown to be affected by oil. Rhodopsin mRNA expresssion was significantly decreased by both compounds equally. However, exposure to 2-OHCHR significantly increased the expression of the hematopoietic regulator, runx1 (runt related transcription factor 1). These results indicate the toxicity of oxygenated photoproducts of PAHs and suggest that other targets and signaling pathways may contribute to developmental toxicity of weathered oil. Our findings also demonstrate the regio-selective toxicity of hydroxy-PAHs in the effects on eye and circulatory development and raise the need to identify mechanisms and ecological risks of oxy-PAHs to fish populations.
[Mh] Termos MeSH primário: Crisenos/toxicidade
Embrião não Mamífero/efeitos dos fármacos
Petróleo/toxicidade
Poluentes Químicos da Água/toxicidade
Peixe-Zebra/embriologia
[Mh] Termos MeSH secundário: Animais
Crisenos/metabolismo
Relação Dose-Resposta a Droga
Embrião não Mamífero/anormalidades
Embrião não Mamífero/metabolismo
Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos
Coração/efeitos dos fármacos
Coração/embriologia
Organogênese/efeitos dos fármacos
Organogênese/genética
Poluentes Químicos da Água/metabolismo
Peixe-Zebra/anormalidades
Peixe-Zebra/genética
Peixe-Zebra/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chrysenes); 0 (Petroleum); 0 (Water Pollutants, Chemical); 37515-51-8 (6-hydroxychrysene)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170611
[St] Status:MEDLINE


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[PMID]:28575812
[Au] Autor:Grova N; Faÿs F; Hardy EM; Appenzeller BMR
[Ad] Endereço:Human Biomonitoring Research Unit, Department of Population Health, Luxembourg Institute of Health, 29, rue Henri Koch, L-4354 Esch-sur-Alzette, Luxembourg. Electronic address: nathalie.grova@lih.lu.
[Ti] Título:New insights into urine-based assessment of polycyclic aromatic hydrocarbon-exposure from a rat model: Identification of relevant metabolites and influence of elimination kinetics.
[So] Source:Environ Pollut;228:484-495, 2017 Sep.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A gas chromatography tandem mass-spectrometry method dedicated to the analysis of 50 metabolites of polycyclic aromatic hydrocarbons (OH-PAHs) was applied to urine specimens collected from female Long Evans rats under controlled exposure to a mixture of PAHs (at 7 doses ranging from 0.01 to 0.8 mg/kg, by gavage, 3 times per week for 90 days). On four occasions (day 1, 28, 60 and 90), urine samples were collected over a 24 h period. Among these 50 OH-PAHs, 41 were detected in urine samples. Seven additional OH-PAHs were identified for the first time: 1 corresponding to metabolite of pyrene and 3 of anthracene. Strong linear dose versus urinary concentration relationships were observed for 25 of the 41 OH-PAHs detected in rat urine, confirming their suitability for assessing exposure to their respective parent compound. In addition, some isomers (e.g. 1-OH-pyrene, 3-OH-/4-OH-chrysene, 10-OH-benz[a]anthracene, 8-OH-benzo[k]fluoranthene, 11-OH-benzo[b]fluoranthene and 3-OH-benzo[a]pyrene) that were detected starting from the lowest levels of exposure or even in controls were considered particularly relevant biomarkers compared to metabolites only detected at higher levels of exposure. Finally, on the basis of the excretion profiles (on days 1, 28, 60 and 90) and urinary elimination kinetics of each OH-PAH detected at days 1 and 60, this study highlighted the fact that sampling time may influence the measurement of metabolites in urine. Taken together, these results provide interesting information on the suitability of the analysis of OH-PAHs in urine for the assessment of PAH exposure, which could be taken into consideration for the design of epidemiological studies in the future.
[Mh] Termos MeSH primário: Poluentes Ambientais/urina
Hidrocarbonetos Aromáticos Policíclicos/urina
[Mh] Termos MeSH secundário: Animais
Antracenos
Benzo(a)pireno/análise
Biomarcadores/urina
Líquidos Corporais
Crisenos
Feminino
Cinética
Pirenos
Ratos
Ratos Long-Evans
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthracenes); 0 (Biomarkers); 0 (Chrysenes); 0 (Environmental Pollutants); 0 (Polycyclic Aromatic Hydrocarbons); 0 (Pyrenes); 084HCM49PT (chrysene); 3417WMA06D (Benzo(a)pyrene); 9E0T7WFW93 (pyrene); EH46A1TLD7 (anthracene)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE


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[PMID]:28236708
[Au] Autor:Santonicola S; De Felice A; Cobellis L; Passariello N; Peluso A; Murru N; Ferrante MC; Mercogliano R
[Ad] Endereço:Department of Veterinary Medicine and Animal Production, University of Naples, Italy.
[Ti] Título:Comparative study on the occurrence of polycyclic aromatic hydrocarbons in breast milk and infant formula and risk assessment.
[So] Source:Chemosphere;175:383-390, 2017 May.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The study compared the polycyclic aromatic hydrocarbons (PAH) profile of human milk collected from Italian mothers and different brands of infant formula available on Italian market. Levels of 14 PAHs most frequently occurred in food, PAH markers listed by Commission Regulation (EC) No. 1881/2006, and carcinogenic PAHs classified by the International Agency for Research on Cancer, were determined by high-pressure liquid chromatography with fluorescence detector. The average concentrations of total PAHs were 114.93 in breast milk and 53.68 µg kg in infant formula. Furthermore, Benzo(a)pyrene (BaP) and the sum of ∑PAH4 markers (BaP, Chrysene, Benzo(a,h)anthracene and Benzo(b)fluoranthene) were higher than the permissible limit of 1 µg kg in 43% and 86% for breast milk and in 10% and 76% for infant formula samples, respectively. Breast milk showed higher levels (P < 0.05) of carcinogenic, and possible carcinogenic hydrocarbons than infant formula samples. Both in human and commercial milk, data showed the occurrence of low and high molecular weight PAHs, respectively from petrogenic and pyrolytic environmental sources, characterizing the infant and mother exposure. Particularly, waste incineration could have represented an important exposure source for infants during breastfeeding, through exposition of mothers resident in some areas of Southern Italy. High PAH levels detected in infant formula enriched with LC-PUFA might be related to the contamination of the vegetable oils added as ingredients. Results showed a high percentage of samples of both breast milk and infant formulas with margin of exposure (MOE) value indicating a potential concern for consumer health.
[Mh] Termos MeSH primário: Contaminação de Alimentos/análise
Fórmulas Infantis/química
Leite Humano/química
Hidrocarbonetos Aromáticos Policíclicos/análise
[Mh] Termos MeSH secundário: Benzo(a)pireno/análise
Cromatografia Líquida de Alta Pressão
Crisenos/análise
Feminino
Fluorenos/análise
Contaminação de Alimentos/legislação & jurisprudência
Regulamentação Governamental
Seres Humanos
Lactente
Fórmulas Infantis/normas
Itália
Óleos Vegetais/química
Medição de Risco
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chrysenes); 0 (Fluorenes); 0 (Plant Oils); 0 (Polycyclic Aromatic Hydrocarbons); 084HCM49PT (chrysene); 3417WMA06D (Benzo(a)pyrene); FJO154KG1X (benzo(b)fluoranthene)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170518
[Lr] Data última revisão:
170518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170226
[St] Status:MEDLINE


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[PMID]:28119020
[Au] Autor:Smith JN; Mehinagic D; Nag S; Crowell SR; Corley RA
[Ad] Endereço:Pacific Northwest National Laboratory, Richland, WA 99352, USA. Electronic address: jordan.smith@pnnl.gov.
[Ti] Título:In vitro metabolism of benzo[a]pyrene-7,8-dihydrodiol and dibenzo[def,p]chrysene-11,12 diol in rodent and human hepatic microsomes.
[So] Source:Toxicol Lett;269:23-32, 2017 Mar 05.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Polycyclic aromatic hydrocarbons (PAHs) are contaminants that are ubiquitously found in the environment, produced through combustion of organic matter or petrochemicals, and many of which are procarcinogens. The prototypic PAH, benzo[a]pyrene (B[a]P) and the highly carcinogenic dibenzo[def,p]chrysene (DBC) are metabolically activated by isoforms of the P450 enzyme superfamily producing benzo[a]pyrene-7,8-dihydrodiol (B[a]P diol), dibenzo[def,p]chrysene-11,12 diol (DBC diol). Each of these diols can be further metabolized by cytochrome P450 enzymes to highly reactive diol-epoxide metabolites that readily react with DNA or by phase II conjugation facilitating excretion. To complement prior in vitro metabolism studies with parent B[a]P and DBC, both phase I metabolism and phase II glucuronidation of B[a]P diol and DBC diol were measured in hepatic microsomes from female B6129SF1/J mice, male Sprague-Dawley rats, and female humans. Metabolic parameters, including intrinsic clearance and Michaelis-Menten kinetics were calculated from substrate depletion data. Mice and rats demonstrated similar B[a]P diol phase I metabolic rates. Compared to rodents, human phase I metabolism of B[a]P diol demonstrated lower overall metabolic capacity, lower intrinsic clearance at higher substrate concentrations (>0.14µM), and higher intrinsic clearance at lower substrate concentrations (<0.07µM). Rates of DBC diol metabolism did not saturate in mice or humans and were highest overall in mice. Higher affinity constants and lower capacities were observed for DBC diol glucuronidation compared to B[a]P diol glucuronidation; however, intrinsic clearance values for these compounds were consistent within each species. Kinetic parameters reported here will be used to extend physiologically based pharmacokinetic (PBPK) models to include the disposition of B[a]P and DBC metabolites in animal models and humans to support future human health risk assessments.
[Mh] Termos MeSH primário: Crisenos/metabolismo
Di-Hidroxi-Di-Hidrobenzopirenos/metabolismo
Microssomos Hepáticos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Benzo(a)pireno/metabolismo
Carcinógenos/metabolismo
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Feminino
Masculino
Camundongos
Microssomos Hepáticos/metabolismo
Hidrocarbonetos Aromáticos Policíclicos/metabolismo
Ratos
Ratos Sprague-Dawley
Medição de Risco
Testes de Toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carcinogens); 0 (Chrysenes); 0 (Dihydroxydihydrobenzopyrenes); 0 (Polycyclic Aromatic Hydrocarbons); 13345-25-0 (benzo(a)pyrene 7,8-dihydrodiol); 3417WMA06D (Benzo(a)pyrene); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE


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[PMID]:28107655
[Au] Autor:Murakami Y; Shibata T; Ueda K
[Ad] Endereço:Graduate School of Engineering, Yokohama National University, Yokohama 240-8501, Japan.
[Ti] Título:Discrimination between naphthacene and triphenylene using cellulose tris(4-methylbenzoate) and cellulose tribenzoate: A computational study.
[So] Source:Carbohydr Res;439:35-43, 2017 Feb 01.
[Is] ISSN:1873-426X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The mechanisms of naphthacene and triphenylene discrimination using commercially available cellulose tris(4-methylbenzoate) (CMB) and cellulose tribenzoate (CB) chiral stationary phases were investigated using molecular mechanics calculations. Naphthacene and triphenylene could be separated by liquid chromatography on CMB and CB, with triphenylene being eluted earlier than naphthacene on both phases. However, the corresponding separation factor is much larger for CMB than for CB. The docking of these polycyclic aromatic hydrocarbons to the above polymers suggested that the most important sites of CMB and CB for interacting with these hydrocarbons are located at equivalent positions, featuring a space surrounded by main chain glucose units and benzoyl side chains. The difference of hydrocarbon stabilization energies with CMB and CB agreed well with the observed chromatographic separation factors.
[Mh] Termos MeSH primário: Benzoatos/química
Celulose/análogos & derivados
Crisenos/isolamento & purificação
Naftacenos/isolamento & purificação
[Mh] Termos MeSH secundário: Sítios de Ligação
Configuração de Carboidratos
Celulose/química
Cromatografia Líquida de Alta Pressão
Crisenos/química
Simulação de Acoplamento Molecular
Naftacenos/química
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzoates); 0 (Chrysenes); 0 (Naphthacenes); 0 (tris(4-methylbenzoate)cellulose); 18WX3373I0 (triphenylene); 9004-34-6 (Cellulose); QYJ5Z6712R (naphthacene)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE


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[PMID]:28083890
[Au] Autor:Awaad AS; Al-Mudhayyif HA; Al-Othman MR; Zain ME; El-Meligy RM
[Ad] Endereço:Pharmacognosy Department, College of Pharmacy, Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia.
[Ti] Título:Amhezole, A Novel Fungal Secondary Metabolite from Aspergillus terreus for Treatment of Microbial Mouth Infection.
[So] Source:Phytother Res;31(3):395-402, 2017 Mar.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bio-guided fractionation of Aspergillus terreus extract leads to isolation of a novel terpenoidal secondary metabolite. The isolated compound and the total alcoholic extract of Aspergillus terreus showed a remarkable activity against microbial mouth infections; namely, Candida albicans, Lactobacillus acidophilus, Streptococcus gordonii, and S. mutan. Moreover, the Minimum Inhibitory Concentration of the isolated compound was determined and showed low values. The combination of each of the alcoholic extract of A. terreus and the isolated compound Coe-Comfort tissue conditioner inhibited the growth of Candida albicans at concentrations of 500 and 7.81 µg/mL, respectively, Lactobacillus acidophilus at concentrations of 250 and 7.81 µg/mL, respectively, Streptococcus gordonii at concentrations of 1000 and 62.50 µg/mL, respectively, and S. mutans at concentrations of 1000 and 125 µg/mL, respectively. The oral dosing of the extract and the isolated compound did not show any significant effect on the activity of alanine aminotransferase, aspirate aminotransferase, and the levels of blood urea and serum creatinine. Copyright © 2017 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Anti-Infecciosos/isolamento & purificação
Anti-Infecciosos/uso terapêutico
Aspergillus/química
Crisenos/uso terapêutico
Infecção/tratamento farmacológico
Doenças da Boca/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/toxicidade
Aspergillus/metabolismo
Candida albicans/efeitos dos fármacos
Candida albicans/crescimento & desenvolvimento
Crisenos/isolamento & purificação
Crisenos/toxicidade
Lactobacillus acidophilus/efeitos dos fármacos
Lactobacillus acidophilus/crescimento & desenvolvimento
Masculino
Testes de Sensibilidade Microbiana
Boca/efeitos dos fármacos
Boca/microbiologia
Ratos
Ratos Wistar
Testes de Toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Chrysenes); 0 (amhezole)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170114
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5760


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[PMID]:28013065
[Au] Autor:Williams DE; Fischer CM; Kassai M; Gude L; Fernández MJ; Lorente A; Grant KB
[Ad] Endereço:Department of Chemistry, Georgia State University, P.O. Box 3965, Atlanta, GA 30302-3965, United States.
[Ti] Título:An unlikely DNA cleaving agent: A photo-active trinuclear Cu(II) complex based on hexaazatriphenylene.
[So] Source:J Inorg Biochem;168:55-66, 2017 Mar.
[Is] ISSN:1873-3344
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This paper describes the synthesis of a trinuclear Cu(II) complex (4) containing a central 1,4,5,8,9,12-hexaazatriphenylene-hexacarboxylate (hat) core (3). Low, micromolar concentrations of the negatively charged parent ligand 3 and the neutral trinuclear complex 4 were found to photocleave negatively charged pUC19 plasmid DNA with high efficiency at neutral pH (350nm, 50min, 22°C). The interactions of complex 4 with double-helical DNA were studied in detail. Scavenger and colorimetric assays pointed to the formation of Cu(I), superoxide anion radicals, hydrogen peroxide, and hydroxyl radicals during photocleavage reactions. UV-visible absorption, circular dichroism, DNA thermal denaturation, and fluorescence data suggested that the Cu(II) complex contacts double-stranded DNA in an external fashion. The persistent association of ligand 3 and complex 4 with Na(I) and/or other cations in aqueous solution might facilitate electrostatic DNA interactions.
[Mh] Termos MeSH primário: Compostos Aza/química
Compostos Aza/farmacologia
Crisenos/química
Crisenos/farmacologia
Cobre/química
Cobre/farmacologia
DNA/efeitos dos fármacos
DNA/metabolismo
Processos Fotoquímicos
[Mh] Termos MeSH secundário: Dicroísmo Circular
Colorimetria
Peróxido de Hidrogênio/química
Estrutura Molecular
Superóxidos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aza Compounds); 0 (Chrysenes); 0 (azatriphenylene); 11062-77-4 (Superoxides); 789U1901C5 (Copper); 9007-49-2 (DNA); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161226
[St] Status:MEDLINE


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[PMID]:28000964
[Au] Autor:Lee YS; Lo JC; Otton SV; Moore MM; Kennedy CJ; Gobas FAPC
[Ad] Endereço:School of Resource and Environmental Management, Simon Fraser University, Burnaby, British Columbia, Canada.
[Ti] Título:In vitro to in vivo extrapolation of biotransformation rates for assessing bioaccumulation of hydrophobic organic chemicals in mammals.
[So] Source:Environ Toxicol Chem;36(7):1934-1946, 2017 Jul.
[Is] ISSN:1552-8618
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Incorporating biotransformation in bioaccumulation assessments of hydrophobic chemicals in both aquatic and terrestrial organisms in a simple, rapid, and cost-effective manner is urgently needed to improve bioaccumulation assessments of potentially bioaccumulative substances. One approach to estimate whole-animal biotransformation rate constants is to combine in vitro measurements of hepatic biotransformation kinetics with in vitro to in vivo extrapolation (IVIVE) and bioaccumulation modeling. An established IVIVE modeling approach exists for pharmaceuticals (referred to in the present study as IVIVE-Ph) and has recently been adapted for chemical bioaccumulation assessments in fish. The present study proposes and tests an alternative IVIVE-B technique to support bioaccumulation assessment of hydrophobic chemicals with a log octanol-water partition coefficient (K ) ≥ 4 in mammals. The IVIVE-B approach requires fewer physiological and physiochemical parameters than the IVIVE-Ph approach and does not involve interconversions between clearance and rate constants in the extrapolation. Using in vitro depletion rates, the results show that the IVIVE-B and IVIVE-Ph models yield similar estimates of rat whole-organism biotransformation rate constants for hypothetical chemicals with log K ≥ 4. The IVIVE-B approach generated in vivo biotransformation rate constants and biomagnification factors (BMFs) for benzo[a]pyrene that are within the range of empirical observations. The proposed IVIVE-B technique may be a useful tool for assessing BMFs of hydrophobic organic chemicals in mammals. Environ Toxicol Chem 2017;36:1934-1946. © 2016 SETAC.
[Mh] Termos MeSH primário: Modelos Teóricos
Compostos Orgânicos/metabolismo
[Mh] Termos MeSH secundário: Animais
Benzo(a)pireno/metabolismo
Biotransformação
Crisenos/metabolismo
Interações Hidrofóbicas e Hidrofílicas
Cinética
Fígado/metabolismo
Mamíferos/metabolismo
Compostos Orgânicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chrysenes); 0 (Organic Chemicals); 084HCM49PT (chrysene); 3417WMA06D (Benzo(a)pyrene)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE
[do] DOI:10.1002/etc.3718


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Fotocópia
Torres, Elizabeth Aparecida Ferraz da Silva
Texto completo
[PMID]:27979277
[Au] Autor:Silva SAD; Sampaio GR; Torres EAFDS
[Ad] Endereço:Department of Nutrition, School of Public Health, University of São Paulo - USP, Avenida Doutor Arnaldo, 715, Cerqueira César, CEP 01246-904 São Paulo, SP, Brazil. Electronic address: simone.alves.silva@usp.br.
[Ti] Título:Optimization and validation of a method using UHPLC-fluorescence for the analysis of polycyclic aromatic hydrocarbons in cold-pressed vegetable oils.
[So] Source:Food Chem;221:809-814, 2017 Apr 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Among the different food categories, the oils and fats are important sources of exposure to polycyclic aromatic hydrocarbons (PAHs), a group of organic chemical contaminants. The use of a validated method is essential to obtain reliable analytical results since the legislation establishes maximum limits in different foods. The objective of this study was to optimize and validate a method for the quantification of four PAHs [benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(a)pyrene] in vegetable oils. The samples were submitted to liquid-liquid extraction, followed by solid-phase extraction, and analyzed by ultra-high performance liquid chromatography. Under the optimized conditions, the validation parameters were evaluated according to the INMETRO Guidelines: linearity (r2 >0.99), selectivity (no matrix interference), limits of detection (0.08-0.30µgkg ) and quantification (0.25-1.00µgkg ), recovery (80.13-100.04%), repeatability and intermediate precision (<10% RSD). The method was found to be adequate for routine analysis of PAHs in the vegetable oils evaluated.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão
Óleos Vegetais/química
Hidrocarbonetos Aromáticos Policíclicos/análise
[Mh] Termos MeSH secundário: Antracenos/análise
Benzo(a)pireno/análise
Crisenos/análise
Dimetilformamida/análise
Fluorenos/análise
Contaminação de Alimentos/análise
Extração Líquido-Líquido
Extração em Fase Sólida
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Anthracenes); 0 (Chrysenes); 0 (Fluorenes); 0 (Plant Oils); 0 (Polycyclic Aromatic Hydrocarbons); 084HCM49PT (chrysene); 3417WMA06D (Benzo(a)pyrene); 8696NH0Y2X (Dimethylformamide); EH46A1TLD7 (anthracene); FJO154KG1X (benzo(b)fluoranthene)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161217
[St] Status:MEDLINE



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