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Pesquisa : D02.455.426.559.847.799.306 [Categoria DeCS]
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[PMID]:29183043
[Au] Autor:Kamiya T; Nagaoka T; Omae T; Ono S; Otani S; Yoshida A
[Ad] Endereço:Department of Ophthalmology, Asahikawa Medical University, Asahikawa, Japan.
[Ti] Título:Benzo(e)pyrene Inhibits Endothelium-Dependent NO-Mediated Dilation of Retinal Arterioles via Superoxide Production and Endoplasmic Reticulum Stress.
[So] Source:Invest Ophthalmol Vis Sci;58(13):5978-5984, 2017 Nov 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: To investigate whether benzo(e)pyrene (B(e)P), a toxicant in cigarette smoke, affects the endothelium-dependent nitric oxide (NO)-induced vasodilation of the retinal arterioles, and whether oxidative stress, distinct protein kinase signaling pathways, and endoplasmic reticulum (ER) stress are associated with the B(e)P-induced effect on the retinal arterioles. Methods: In this in vitro study, porcine retinal arterioles were isolated, cannulated, and pressurized without flow. These vessels were treated with intraluminal administration of B(e)P or B(e)P plus blockers for 180 minutes. Diametric changes to agonists were recorded by videomicroscopy. Results: Intraluminal treatment with 100 µM B(e)P for 180 minutes significantly reduced the arteriolar vasodilation caused by the endothelium-dependent NO-mediated agonists bradykinin and A23187 but not that caused by endothelium-independent NO donor sodium nitroprusside. The adverse effects of B(e)P on the vasodilatory action of bradykinin were prevented by the superoxide scavenger 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPOL), the nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase) inhibitor apocynin, the c-Jun N-terminal kinase (JNK) inhibitor SP600125, the p38 mitogen-activated protein kinase inhibitor SB203580, genistein, resveratrol (RSV), and the ER stress inhibitor 4-phenylbutyrate (4-PBA). The xanthine oxidase inhibitor allopurinol did not alter the effect of B(e)P on the vasodilatory action induced by bradykinin. Conclusions: B(e)P decreases the endothelium-dependent NO-induced vasodilation in the retinal arterioles through the production of superoxide from NADPH oxidase, which is linked to JNK and p38 kinase. The results suggested that ER stress is instrumental in B(e)P-induced endothelial dysfunction and that genistein and RSV might preserve endothelial function.
[Mh] Termos MeSH primário: Arteríolas/efeitos dos fármacos
Benzopirenos/farmacologia
Estresse do Retículo Endoplasmático/fisiologia
Vasos Retinianos/efeitos dos fármacos
Superóxidos/metabolismo
Vasodilatação/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antioxidantes/farmacologia
Bradicinina/farmacologia
Óxidos N-Cíclicos/farmacologia
Modelos Animais de Doenças
Marcadores de Spin
Suínos
Vasodilatadores/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Benzopyrenes); 0 (Cyclic N-Oxides); 0 (Spin Labels); 0 (Vasodilator Agents); 11062-77-4 (Superoxides); 63APT6398R (benzo(e)pyrene); S8TIM42R2W (Bradykinin); U78ZX2F65X (tempol)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-21925


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[PMID]:28709111
[Au] Autor:Han J; Kim DH; Kim HS; Nelson DR; Lee JS
[Ad] Endereço:Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
[Ti] Título:Genome-wide identification of 52 cytochrome P450 (CYP) genes in the copepod Tigriopus japonicus and their B[α]P-induced expression patterns.
[So] Source:Comp Biochem Physiol Part D Genomics Proteomics;23:49-57, 2017 Sep.
[Is] ISSN:1878-0407
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Cytochrome P450s (CYPs) are enzymes with a heme-binding domain that are found in all living organisms. CYP enzymes have important roles associated with detoxification of xenobiotics and endogenous compounds (e.g. steroids, fatty acids, and hormones). Although CYP enzymes have been reported in several invertebrates, including insects, little is known about copepod CYPs. Here, we identified the entire repertoire of CYP genes (n=52) from whole genome and transcriptome sequences of the benthic copepod Tigriopus japonicus, including a tandem duplication (CYP3026A3, CYP3026A4, CYP3026A5), and examined patterns of gene expression over various developmental stages and in response to benzo[α]pyrene (B[α]P) exposure. Through phylogenetic analysis, the 52 T. japonicus CYP genes were assigned to five distinct clans: CYP2 (22 genes), CYP3 (19 genes), CYP4 (two genes), CYP20 (one gene), and mitochondrial (eight genes). Developmental stage and gender-specific expression patterns of the 52 T. japonicus CYPs were analyzed. CYP3022A1 was constitutively expressed during all developmental stages. CYP genes in clans 2 and 3 were induced in response to B[α]P, suggesting that these differentially modulated CYP transcripts are likely involved in defense against exposure to B[α]P and other pollutants. This study enhances our understanding of the repertoire of CYP genes in copepods and of their potential role in development and detoxification in copepods.
[Mh] Termos MeSH primário: Benzopirenos/farmacologia
Copépodes/efeitos dos fármacos
Copépodes/genética
Sistema Enzimático do Citocromo P-450/genética
Transcriptoma/efeitos dos fármacos
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Copépodes/classificação
Filogenia
Alinhamento de Sequência
Poluentes Químicos da Água/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzopyrenes); 0 (Water Pollutants, Chemical); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE


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[PMID]:28477566
[Au] Autor:Kim HS; Han J; Kim HJ; Hagiwara A; Lee JS
[Ad] Endereço:Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
[Ti] Título:Identification of 28 cytochrome P450 genes from the transcriptome of the marine rotifer Brachionus plicatilis and analysis of their expression.
[So] Source:Comp Biochem Physiol Part D Genomics Proteomics;23:1-7, 2017 Sep.
[Is] ISSN:1878-0407
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Whole transcriptomes of the rotifer Brachionus plicatilis were analyzed using an Illumina sequencer. De novo assembly was performed with 49,122,780 raw reads using Trinity software. Among the assembled 42,820 contigs, 27,437 putative open reading frame contigs were identified (average length 1235bp; N50=1707bp). Functional gene annotation with Gene Ontology and InterProScan, in addition to Kyoto Encyclopedia of Genes and Genomes pathway analysis, highlighted the metabolism of xenobiotics by cytochrome P450 (CYP). In addition, 28 CYP genes were identified, and their transcriptional responses to benzo[α]pyrene (B[α]P) were investigated. Most of the CYPs were significantly upregulated or downregulated (P<0.05) in response to B[α]P, suggesting that Bp-CYP genes play a crucial role in detoxification mechanisms in response to xenobiotics. This study sheds light on the molecular defense mechanisms of the rotifer B. plicatilis in response to exposure to various chemicals.
[Mh] Termos MeSH primário: Sistema Enzimático do Citocromo P-450/genética
Regulação da Expressão Gênica
Rotíferos/genética
Transcriptoma/genética
[Mh] Termos MeSH secundário: Animais
Benzopirenos/farmacologia
Sistema Enzimático do Citocromo P-450/fisiologia
Mecanismos de Defesa
Perfilação da Expressão Gênica
Regulação da Expressão Gênica/efeitos dos fármacos
Biologia Marinha
Alinhamento de Sequência
Poluentes Químicos da Água/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzopyrenes); 0 (Water Pollutants, Chemical); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170507
[St] Status:MEDLINE


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[PMID]:28402640
[Au] Autor:Gowda ASP; Krzeminski J; Amin S; Suo Z; Spratt TE
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Milton S. Hershey Medical Center, Pennsylvania State University College of Medicine , Hershey, Pennsylvania 17033, United States.
[Ti] Título:Mutagenic Replication of N -Deoxyguanosine Benzo[a]pyrene Adducts by Escherichia coli DNA Polymerase I and Sulfolobus solfataricus DNA Polymerase IV.
[So] Source:Chem Res Toxicol;30(5):1168-1176, 2017 May 15.
[Is] ISSN:1520-5010
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Benzo[a]pyrene, a potent human carcinogen, is metabolized in vivo to a diol epoxide that reacts with the N -position of guanine to produce N -BP-dG adducts. These adducts are mutagenic causing G to T transversions. These adducts block replicative polymerases but can be bypassed by the Y-family translesion synthesis polymerases. The mechanisms by which mutagenic bypass occurs is not well-known. We have evaluated base pairing structures using atomic substitution of the dNTP with two stereoisomers, 2'-deoxy-N-[(7R,8S,9R,10S)-7,8,9,10-tetrahydro-7,8,9-trihydroxybenzo[a]pyren-10-yl]guanosine and 2'-deoxy-N-[(7S,8R,9S,10R)-7,8,9,10-tetrahydro-7,8,9-trihydroxybenzo[a]pyren-10-yl]guanosine. We have examined the kinetics of incorporation of 1-deaza-dATP, 7-deaza-dATP, 2'-deoxyinosine triphosphate, and 7-deaza-dGTP, analogues of dATP and dGTP in which single atoms are changed. Changes in rate will occur if that atom provided a critical interaction in the transition state of the reaction. We examined two polymerases, Escherichia coli DNA polymerase I (Kf) and Sulfolobus solfataricus DNA polymerase IV (Dpo4), as models of a high fidelity and TLS polymerase, respectively. We found that with Kf, substitution of the nitrogens on the Watson-Crick face of the dNTPs resulted in decreased rate of reactions. This result is consistent with a Hoogsteen base pair in which the template N -BP-dG flipped from the anti to syn conformation. With Dpo4, while the substitution did not affect the rate of reaction, the amplitude of the reaction decreased with all substitutions. This result suggests that Dpo4 bypasses N -BP-dG via Hoogsteen base pairs but that the flipped nucleotide can be either the dNTP or the template.
[Mh] Termos MeSH primário: Benzopirenos/metabolismo
Adutos de DNA
DNA Polimerase I/metabolismo
DNA Polimerase beta/metabolismo
Replicação do DNA
Desoxiguanosina/análogos & derivados
Escherichia coli/enzimologia
Sulfolobus solfataricus/enzimologia
[Mh] Termos MeSH secundário: Pareamento de Bases
Catálise
Desoxiguanosina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzopyrenes); 0 (DNA Adducts); 0 (benzo(a)pyrene N2-dG adduct); EC 2.7.7.- (DNA Polymerase I); EC 2.7.7.- (DNA Polymerase beta); G9481N71RO (Deoxyguanosine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
171108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1021/acs.chemrestox.6b00466


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[PMID]:28319121
[Au] Autor:Liu A; Archer AM; Biggs MB; Papin JA
[Ad] Endereço:Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, United States of America.
[Ti] Título:Growth-altering microbial interactions are responsive to chemical context.
[So] Source:PLoS One;12(3):e0164919, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microbial interactions are ubiquitous in nature, and are equally as relevant to human wellbeing as the identities of the interacting microbes. However, microbial interactions are difficult to measure and characterize. Furthermore, there is growing evidence that they are not fixed, but dependent on environmental context. We present a novel workflow for inferring microbial interactions that integrates semi-automated image analysis with a colony stamping mechanism, with the overall effect of improving throughput and reproducibility of colony interaction assays. We apply our approach to infer interactions among bacterial species associated with the normal lung microbiome, and how those interactions are altered by the presence of benzo[a]pyrene, a carcinogenic compound found in cigarettes. We found that the presence of this single compound changed the interaction network, demonstrating that microbial interactions are indeed dynamic and responsive to local chemical context.
[Mh] Termos MeSH primário: Interações Microbianas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Processamento Automatizado de Dados
Benzo(a)pireno/toxicidade
Benzopirenos/toxicidade
Carcinógenos
Técnicas de Cultura de Células
Haemophilus/citologia
Haemophilus/efeitos dos fármacos
Haemophilus/fisiologia
Seres Humanos
Processamento de Imagem Assistida por Computador
Pulmão/efeitos dos fármacos
Pulmão/microbiologia
Interações Microbianas/fisiologia
Microbiota/efeitos dos fármacos
Microbiota/fisiologia
Microscopia
Pseudomonas aeruginosa/citologia
Pseudomonas aeruginosa/efeitos dos fármacos
Pseudomonas aeruginosa/fisiologia
Staphylococcus aureus/citologia
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus aureus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzopyrenes); 0 (Carcinogens); 3417WMA06D (Benzo(a)pyrene)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0164919


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[PMID]:27886549
[Au] Autor:Bai H; Zhou J; Zhang H; Tang G
[Ad] Endereço:State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou 310028, China.
[Ti] Título:Enhanced adsorbability and photocatalytic activity of TiO -graphene composite for polycyclic aromatic hydrocarbons removal in aqueous phase.
[So] Source:Colloids Surf B Biointerfaces;150:68-77, 2017 Feb 01.
[Is] ISSN:1873-4367
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Photodegradation via titanium dioxide (TiO ) has been used to remove polycyclic aromatic hydrocarbons (PAHs) from environmental media broadly. In this study, a series of TiO -graphene composites (P25-GR) with different GR weight ratios were synthesized via hydrothermal reaction of graphene oxide (GO) and P25. Their structures were characterized and the proprieties were tested in aqueous phase. Phenanthrene (PHE), fluoranthene (FLAN), and benzo[a]pyrene (BaP) were selected as models of PAHs. The experiment indicated that P25-2.5%GR exhibited enhancement in both adsorption and photodegradation, ∼80% of PAHs were removed after 2h photocatalysis. The influence of photodegradation rate was studied, including PAHs initial concentration and pH. Aromatic intermediates were identified during the reaction process and the degradation pathways were portrayed. This work explored the enhanced photocatalysis performance was attributed to the PAH-selective adsorbability and the strong electron transfer ability of the composite. The analysis of the degradation intermediates confirmed that the reaction proceeded with the formation of free radicals, leading to the gradual PAH mineralization.
[Mh] Termos MeSH primário: Fotoquímica/métodos
Hidrocarbonetos Aromáticos Policíclicos/química
Titânio/química
[Mh] Termos MeSH secundário: Adsorção
Benzopirenos/química
Catálise
Poluentes Ambientais/química
Recuperação e Remediação Ambiental
Fluorenos/química
Radicais Livres
Grafite/química
Cinética
Óxidos/química
Fenantrenos/química
Fotólise
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzopyrenes); 0 (Environmental Pollutants); 0 (Fluorenes); 0 (Free Radicals); 0 (Oxides); 0 (Phenanthrenes); 0 (Polycyclic Aromatic Hydrocarbons); 059QF0KO0R (Water); 15FIX9V2JP (titanium dioxide); 360UOL779Z (fluoranthene); 448J8E5BST (phenanthrene); 7782-42-5 (Graphite); D1JT611TNE (Titanium)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE


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[PMID]:27849171
[Au] Autor:Izawa T; Arakaki R; Mori H; Tsunematsu T; Kudo Y; Tanaka E; Ishimaru N
[Ad] Endereço:Department of Orthodontics and Dentofacial Orthopedics, Tokushima University Graduate School of Biomedical Sciences, Tokushima 7708504, Japan; and.
[Ti] Título:The Nuclear Receptor AhR Controls Bone Homeostasis by Regulating Osteoclast Differentiation via the RANK/c-Fos Signaling Axis.
[So] Source:J Immunol;197(12):4639-4650, 2016 Dec 15.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aryl hydrocarbon receptor (AhR) pathway plays a key role in receptor activator of NF-κB ligand (RANKL)-mediated osteoclastogenesis. However, the mechanism underlying the regulation of AhR expression in osteoclasts and the signaling pathway through which AhR controls osteoclastogenesis remain unclear. We found that the expression of AhR in bone marrow-derived osteoclasts was upregulated by RANKL at an earlier stage than was the expression of signature osteoclast genes such as those encoding cathepsin K and NFAT, cytoplasmic, calcineurin-dependent 1. In response to RANKL, bone marrow macrophages isolated from AhR mice exhibited impaired phosphorylation of Akt and MAPK as well as NF-κB, whereas their response to M-CSF remained unchanged. Osteoclast differentiation mediated by the AhR signaling pathway was also regulated in an RANKL/c-Fos-dependent manner. Furthermore, ligand activation of AhR by the smoke toxin benzo[a]pyrene accelerated osteoclast differentiation in a receptor-dependent manner, and AhR-dependent regulation of mitochondrial biogenesis in osteoclasts was observed. Moreover, AhR mice exhibited impaired bone healing with delayed endochondral ossification. Taken together, the present results suggest that the RANKL/AhR/c-Fos signaling axis plays a critical role in osteoclastogenesis, thereby identifying the potential of AhR in treating pathological, inflammatory, or metabolic disorders of the bone.
[Mh] Termos MeSH primário: Mitocôndrias/metabolismo
Osteoclastos/fisiologia
Osteogênese
Receptores de Hidrocarboneto Arílico/metabolismo
[Mh] Termos MeSH secundário: Animais
Benzopirenos/metabolismo
Células da Medula Óssea/fisiologia
Células Cultivadas
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
NF-kappa B/metabolismo
Osteogênese/genética
Proteínas Proto-Oncogênicas c-fos/metabolismo
Ligante RANK/metabolismo
Receptores de Hidrocarboneto Arílico/genética
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzopyrenes); 0 (NF-kappa B); 0 (Proto-Oncogene Proteins c-fos); 0 (RANK Ligand); 0 (Receptors, Aryl Hydrocarbon); 0 (Tnfsf11 protein, mouse)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:161117
[St] Status:MEDLINE


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[PMID]:27494294
[Au] Autor:Madeen EP; Ognibene TJ; Corley RA; McQuistan TJ; Henderson MC; Baird WM; Bench G; Turteltaub KW; Williams DE
[Ad] Endereço:Department of Environmental and Molecular Toxicology, Oregon State University , Corvallis, Oregon 97331, United States.
[Ti] Título:Human Microdosing with Carcinogenic Polycyclic Aromatic Hydrocarbons: In Vivo Pharmacokinetics of Dibenzo[def,p]chrysene and Metabolites by UPLC Accelerator Mass Spectrometry.
[So] Source:Chem Res Toxicol;29(10):1641-1650, 2016 Oct 17.
[Is] ISSN:1520-5010
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Metabolism is a key health risk factor following exposures to pro-carcinogenic polycyclic aromatic hydrocarbons (PAHs) such as dibenzo[def,p]chrysene (DBC), an IARC classified 2A probable human carcinogen. Human exposure to PAHs occurs primarily from the diet in nonsmokers. However, little data is available on the metabolism and pharmacokinetics in humans of high molecular weight PAHs (≥4 aromatic rings), including DBC. We previously determined the pharmacokinetics of DBC in human volunteers orally administered a microdose (29 ng; 5 nCi) of [ C]-DBC by accelerator mass spectrometry (AMS) analysis of total [ C] in plasma and urine. In the current study, we utilized a novel "moving wire" interface between ultraperformance liquid chromatography (UPLC) and AMS to detect and quantify parent DBC and its major metabolites. The major [ C] product identified in plasma was unmetabolized [ C]-DBC itself (C = 18.5 ±15.9 fg/mL, T = 2.1 ± 1.0 h), whereas the major metabolite was identified as [ C]-(+/-)-DBC-11,12-diol (C = 2.5 ±1.3 fg/mL, T = 1.8 h). Several minor species of [ C]-DBC metabolites were also detected for which no reference standards were available. Free and conjugated metabolites were detected in urine with [ C]-(+/-)-DBC-11,12,13,14-tetraol isomers identified as the major metabolites, 56.3% of which were conjugated (C = 35.8 ± 23.0 pg/pool, T = 6-12 h pool). [ C]-DBC-11,12-diol, of which 97.5% was conjugated, was also identified in urine (C = 29.4 ± 11.6 pg/pool, T = 6-12 h pool). Parent [ C]-DBC was not detected in urine. This is the first data set to assess metabolite profiles and associated pharmacokinetics of a carcinogenic PAH in human volunteers at an environmentally relevant dose, providing the data necessary for translation of high dose animal models to humans for translation of environmental health risk assessment.
[Mh] Termos MeSH primário: Benzopirenos/metabolismo
Benzopirenos/farmacocinética
[Mh] Termos MeSH secundário: Adulto
Idoso
Benzopirenos/análise
Cromatografia Líquida de Alta Pressão
Relação Dose-Resposta a Droga
Feminino
Voluntários Saudáveis
Seres Humanos
Masculino
Espectrometria de Massas
Meia-Idade
Estrutura Molecular
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzopyrenes); G3X629VE4A (dibenzo(a,l)pyrene)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160806
[St] Status:MEDLINE


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[PMID]:27348178
[Au] Autor:Zurabashvili D; Parulava G; Shanidze L; Kikalishvili B; Nikolaishvili M
[Ad] Endereço:Center of Mental Health and Prevention of Addiction, Tbilisi; Georgian State Teaching University of Physical Education and Sport, Tbilisi, Georgia.
[Ti] Título:[THE LEVEL OF BENZ(A)PIREN IN TOBACCO SMOKE].
[So] Source:Georgian Med News;(254):107-11, 2016 May.
[Is] ISSN:1512-0112
[Cp] País de publicação:Georgia (Republic)
[La] Idioma:rus
[Ab] Resumo:The medical problems of the environmental pollution with products of tobacco smoke are relatively known. The question of separate components of tobacco smoke, factors such a puff-volume, rate, distance, frequency, length of butt in the environment air is not well understand and should further be investigated. It is shown the dependence of the process on the following factors: physic-chemical parameters of atmospheric environment, brand of tobacco product, activity of smoking process. We aimed to determine the dependence of benz(a)pirene in the air samples of tobacco smoke in the distance of 2,0; 4,0 and 6,0m. from lighting cigarette after puff-by puff. Cigarettes were machine-smoked and the total particulate matter was collected (1,0m3) in room, having no air filtration and substances were analysed and identified by gashromatography. The condacted quantitative and qualitative analyses show, that distance of exposition from burning cigarette can change the volume of benz(a)pirene. In the result of pyrolitic and photochemical reactions in tobacco smoke at certain air space temperature new structures can be formed with high toxity and cancerogenity. The dominant transformation process is reaction with photochemically-produced radicals, which produced benz(a)piren as a minor product. Additional factors effecting indoor concentrations include location and ventilation condition time. Ultrafine particle and benz(a)piren deposition and smoking behavior were observed. The mainstream smoke was also monitored continuously in real time (3, 5 and 10 minute) on a puff-by-puff. Our data show that smoking pastime can change the structure and volume of component of tobacco smoke. The level of benz(a)piren in air samples was evaluated as the main background index of cigarette smoke toxity in relatively small room, having no air filtration system. This question still needs to be explained. It would be interesting to investigate of tobacco smoke components in lung tissue after the smoking process. The studies are necessary in different derection.
[Mh] Termos MeSH primário: Poluentes Atmosféricos/análise
Benzopirenos/análise
Fumaça/análise
Tabaco/química
[Mh] Termos MeSH secundário: Poluição por Fumaça de Tabaco/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Air Pollutants); 0 (Benzopyrenes); 0 (Smoke); 0 (Tobacco Smoke Pollution)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160628
[St] Status:MEDLINE


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[PMID]:27338670
[Au] Autor:Suzuki T; Grúz P; Honma M; Adachi N; Nohmi T
[Ad] Endereço:Division of Genetics and Mutagenesis, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan. Electronic address: suzukite@hiroshima-u.ac.jp.
[Ti] Título:Sensitivity of human cells expressing low-fidelity or weak-catalytic-activity variants of DNA polymerase ζ to genotoxic stresses.
[So] Source:DNA Repair (Amst);45:34-43, 2016 Sep.
[Is] ISSN:1568-7856
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Translesion DNA polymerases (TLS pols) play critical roles in defense mechanisms against genotoxic agents. The defects or mutations of TLS pols are predicted to result in hypersensitivity of cells to environmental mutagens. In this study, human cells expressing DNA polymerase ζ (Pol ζ) variants with low fidelity or weak catalytic activity have been established with Nalm-6-MSH+ cells and their sensitivity to mutagenicity and cytotoxicity of benzo[a]pyrene diol epoxide (BPDE) and ultraviolet-C light (UV-C) was examined. The low-fidelity mutants were engineered by knocking-in DNA sequences that direct changes of leucine 2618 to either phenylalanine (L2618F) or methionine (L2618M) of Pol ζ. The weak-catalytic-activity mutants were generated by knocking-in DNA sequences that direct changes of either tyrosine 2779 to phenylalanine (Y2779F) or aspartate 2781 to asparagine (D2781N). In addition, a +1 frameshift mutation, i.e., CCC to CCCC, was introduced in the coding region of the TK1 gene to measure the mutant frequencies. Doubling time and spontaneous TK mutant frequencies of the established cell lines were similar to those of the wild-type cells. The low-fidelity mutants displayed, however, higher sensitivity to the mutagenicity of BPDE and UV-C than the wild-type cells although their cytotoxic sensitivity was not changed. In contrast, the weak-catalytic-activity mutants were more sensitive to the cytotoxicity of BPDE and UV-C than the wild-type cells, and displayed much higher sensitivity to the clastogenicity of BPDE than the wild-type cells in an in vitro micronucleus assay. These results indicate that human Pol ζ is involved in TLS across DNA lesions induced by BPDE and UV-C and also that the TLS plays important roles in induction of mutations, clastogenicity and in cellular survival of the damaged human cells. Similarities and differences in in vivo roles of yeast and human Pol ζ in genome integrity are discussed.
[Mh] Termos MeSH primário: Linfócitos B/metabolismo
Reparo do DNA
Proteínas de Ligação a DNA/genética
DNA Polimerase Dirigida por DNA/genética
DNA/genética
Timidina Quinase/genética
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Linfócitos B/efeitos dos fármacos
Linfócitos B/patologia
Linfócitos B/efeitos da radiação
Sequência de Bases
Benzopirenos/farmacologia
Linhagem Celular Tumoral
Sobrevivência Celular
DNA/metabolismo
Dano ao DNA
Proteínas de Ligação a DNA/metabolismo
DNA Polimerase Dirigida por DNA/metabolismo
Mutação da Fase de Leitura
Expressão Gênica
Seres Humanos
Testes para Micronúcleos
Mutagênese Sítio-Dirigida
Mutagênicos/farmacologia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Tolerância a Radiação
Saccharomyces cerevisiae
Timidina Quinase/metabolismo
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzopyrenes); 0 (DNA-Binding Proteins); 0 (Mutagens); 0 (RNA, Messenger); 60268-85-1 (benzo(a)pyrene diolepoxide I); 9007-49-2 (DNA); EC 2.7.1.21 (Thymidine Kinase); EC 2.7.1.21 (thymidine kinase 1); EC 2.7.7.- (DNA polymerase zeta); EC 2.7.7.7 (DNA-Directed DNA Polymerase); EC 2.7.7.7 (REV3L protein, human)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160625
[St] Status:MEDLINE



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