Base de dados : MEDLINE
Pesquisa : D02.455.426.779.345 [Categoria DeCS]
Referências encontradas : 489 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 49 ir para página                         

  1 / 489 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28260732
[Au] Autor:Awazu K; Takatori S; Kakimoto S; Nomura C; Masayama A; Yamaguchi M; Kakimoto Y; Kajimura K
[Ad] Endereço:Osaka Prefectural Institute of Public Health.
[Ti] Título:Detection of Histamine in Fish and Fishery Products in Osaka Prefecture (Fiscal 2015 Report).
[So] Source:Shokuhin Eiseigaku Zasshi;58(1):43-48, 2017.
[Is] ISSN:1882-1006
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:Histamine food poisoning is caused by ingestion of spoiled fish containing high levels of histamine. This paper reports cases in which histamine was detected in Osaka prefecture in fiscal year 2015 in a survey of fish and fishery products on the market and the food poisoning. A suspected case of histamine food poisoning was also evaluated to investigate the cause and minimize further problems. Histamine in food was separated on SPE cartridge columns, and analyzed after derivatization with fluorescamine by means of HPLC-FL. Histamine was detected in some fishery products on the market and in food that had caused poisoning. The samples in which histamine was detected were semi-dried whole round herring (Urumeiwashi-maruboshi), mackerel (Saba) and sardine dumpling (Iwashi-tsumire). These foods were the main causes of histamine food poisoning according to the report of the Ministry of Health, Labour and Welfare, Government of Japan.
[Mh] Termos MeSH primário: Produtos Pesqueiros/análise
Peixes
Análise de Alimentos/métodos
Contaminação de Alimentos/análise
Doenças Transmitidas por Alimentos/etiologia
Histamina/análise
Histamina/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão/métodos
Fluorescamina
Histamina/efeitos adversos
Seres Humanos
Japão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
38183-12-9 (Fluorescamine); 820484N8I3 (Histamine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170307
[St] Status:MEDLINE
[do] DOI:10.3358/shokueishi.58.43


  2 / 489 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27601175
[Au] Autor:Rana MK; Luthra-Guptasarma M
[Ad] Endereço:Department of Immunopathology, Research Block A, Postgraduate Institute of Medical Education and Research (PGIMER), Sector-12, Chandigarh, 160012, India.
[Ti] Título:Multi-modal Binding of a 'Self' Peptide by HLA-B*27:04 and B*27:05 Allelic Variants, but not B*27:09 or B*27:06 Variants: Fresh Support for Some Theories Explaining Differential Disease Association.
[So] Source:Protein J;35(5):346-353, 2016 Oct.
[Is] ISSN:1875-8355
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A self-derived-peptide with the same amino acid sequence (N-RRYLENGKETLQR-C) as residues 169-181 of the human leukocyte antigen (HLA) B27 heavy chain is known to bind to MHC Class I complexes containing the HLA-B27 heavy chain. This observation has been invoked previously in at least two different (but related) molecular explanations for the disease-association of the HLA-B27 allele. Here, we use a combination of fluorescence polarization, competitive inhibition and gel filtration chromatographic studies to show that a fluorescently-labeled peptide of the above sequence binds to two disease-associated subtypes of HLA-B27 (namely HLA-B*27:04 and HLA-B*27:05) but not to non-disease-associated subtypes (HLA-B*27:06 or HLA-B*27:09). This differential binding behavior is seen both in (a) peptide binding to complexes of heavy chain (HLA-B27) and light chain (ß microglobulin), and in (b) peptide binding to ß microglobulin-free heavy chains in the aggregated state. Such subtype-specific differences are not seen with two other control peptides known to bind to HLA-B27. Our results support the likelihood of differential peptide binding holding at least one of the keys to HLA-B27's disease association.
[Mh] Termos MeSH primário: Antígeno HLA-B27/química
Modelos Imunológicos
Peptídeos/química
[Mh] Termos MeSH secundário: Alelos
Sequência de Aminoácidos
Autoimunidade
Fluorescamina/química
Corantes Fluorescentes/química
Expressão Gênica
Antígeno HLA-B27/genética
Antígeno HLA-B27/imunologia
Seres Humanos
Modelos Moleculares
Peptídeos/genética
Peptídeos/imunologia
Ligação Proteica
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/imunologia
Coloração e Rotulagem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (HLA-B*27:04 antigen); 0 (HLA-B*27:05 antigen); 0 (HLA-B*27:06 antigen); 0 (HLA-B*27:09 antigen); 0 (HLA-B27 Antigen); 0 (Peptides); 0 (Recombinant Proteins); 38183-12-9 (Fluorescamine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE


  3 / 489 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27287055
[Au] Autor:Araujo NA; Guevara A; Lorenzo MA; Calabokis M; Bubis J
[Ad] Endereço:Departamento de Biología Celular, División de Ciencias Biológicas, Universidad Simón Bolívar, Apartado 89.000, Valle de Sartenejas, Baruta, Caracas, 1081, Venezuela. nelsonaaa@gmail.com.
[Ti] Título:Fluram-Kemptide-Lys8 Non-radioactive Assay for Protein Kinase A.
[So] Source:Protein J;35(4):247-55, 2016 08.
[Is] ISSN:1875-8355
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The cAMP-dependent protein kinase (PKA) is the best understood member of the superfamily of serine-threonine protein kinases and is involved in controlling a variety of cellular processes. Measurements of PKA activity traditionally relied on the use of [(32)P]-labeled ATP as the phosphate donor and a protein or peptide substrate as the phosphoaceptor. Recently non-isotopic assays for the PKA have been developed and this paper presents an improvement of a fluorometric assay for measuring the activity of PKA. Three peptides were synthesized with the following sequences: LRRASLG (Kemptide), LRRASLGK (Kemptide-Lys8) and LRRASLGGGLRRASLG (Bis-Kemptide), these have in common the substrate sequence recognized by the PKA (RRXS/TΨ), where X is any amino acid and Ψ is a hydrophobic amino acid. Optimal conditions were established for the non-radioactive assay to detect the PKA activity by phosphorylation of these three peptides that are covalently linked to fluorescamine at their N-terminus. The phosphorylated and non-phosphorylated peptides were easily separated by electrophoresis, identified and quantified with optical densitometry and ultraviolet light. The fluorescamine-labeled Kemptide-Lys8 substrate (Fluram-Kemptide-Lys8) was used to calculate the Km and Vmax of the catalytic subunit of PKA from pig heart and showed a detection limit of 260 pmol, a linear range between 700 and 1150 pmol with a linear regression R (2) = 0.956.
[Mh] Termos MeSH primário: Proteínas Quinases Dependentes de AMP Cíclico/química
Ensaios Enzimáticos/métodos
Fluorescamina/química
Oligopeptídeos/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Domínio Catalítico
Proteínas Quinases Dependentes de AMP Cíclico/isolamento & purificação
Cinética
Miocárdio/química
Oligopeptídeos/síntese química
Fosforilação
Especificidade por Substrato
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Oligopeptides); 38183-12-9 (Fluorescamine); 65189-71-1 (kemptide); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160612
[St] Status:MEDLINE
[do] DOI:10.1007/s10930-016-9667-9


  4 / 489 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26928391
[Au] Autor:Debnath K; Mandal K; Jana NR
[Ad] Endereço:Centre for Advanced Materials, Indian Association for the Cultivation of Science , Kolkata-700032, India.
[Ti] Título:Phase Transfer and Surface Functionalization of Hydrophobic Nanoparticle using Amphiphilic Poly(amino acid).
[So] Source:Langmuir;32(11):2798-807, 2016 Mar 22.
[Is] ISSN:1520-5827
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Functionalization of nanoparticles with chemical and biochemical is essential for their biomedical and other application. However, most of the high quality nanoparticles are hydrophobic in nature due to surfactant capping and their conversion into water-soluble functional nanoparticle via appropriate coating and conjugation chemistry is extremely critical issue. Here we report amphiphilic poly(amino acid)-based one-pot coating and conjugation approach that can transform hydrophobic nanoparticle into water-soluble nanoparticle functionalized with primary amine, thiol, and biomolecule. We have designed amphiphilic polyaspartimide that can anchor hydrophobic nanoparticle through octadecyl groups, leaving the polar polyethylene glycol and aspartimide groups exposed outwards. The aspartimide group is then reacted with primary amine containing chemical/biomolecule with the formation of water-soluble functional nanoparticle. This approach has been extended to different hydrophobic nanoparticles and biomolecules. The present approach has advantages over existing approaches as coating and functionalization can be performed in one pot and functional nanoparticles have <12 nm hydrodynamic size, high colloidal stability, and biocompartibility. This developed approach can be used to derive biocompatible nanobioconjugates for various biomedical applications.
[Mh] Termos MeSH primário: Nanopartículas Metálicas/química
Peptídeos/química
Polietilenoglicóis/química
[Mh] Termos MeSH secundário: Aminas/química
Animais
Antracenos/química
Arginina/análogos & derivados
Arginina/química
Células CHO
Compostos de Cádmio/química
Cricetulus
Ácido Ditionitrobenzoico/química
Compostos Férricos/química
Fluorescamina/química
Nanopartículas Metálicas/toxicidade
Tamanho da Partícula
Fenantrenos/química
Pontos Quânticos/química
Compostos de Selênio/química
Sulfetos/química
Compostos de Zinco/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amines); 0 (Anthracenes); 0 (Cadmium Compounds); 0 (Ferric Compounds); 0 (Peptides); 0 (Phenanthrenes); 0 (Selenium Compounds); 0 (Sulfides); 0 (Zinc Compounds); 1K09F3G675 (ferric oxide); 30IQX730WE (Polyethylene Glycols); 38183-12-9 (Fluorescamine); 42L7BZ8H74 (9,10-phenanthrenequinone); 94ZLA3W45F (Arginine); 9BZQ3U62JX (Dithionitrobenzoic Acid); A7F646JC5C (cadmium selenide); FFV58UNY7O (stearylamine); FP0FJ7K744 (anthrone); KPS085631O (zinc sulfide)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170104
[Lr] Data última revisão:
170104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE
[do] DOI:10.1021/acs.langmuir.6b00282


  5 / 489 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26745872
[Au] Autor:Toro TB; Pingali S; Nguyen TP; Garrett DS; Dodson KA; Nichols KA; Haynes RA; Payton-Stewart F; Watt TJ
[Ad] Endereço:Department of Chemistry, Xavier University of Louisiana, 1 Drexel Dr., New Orleans, Louisiana, United States of America.
[Ti] Título:KDAC8 with High Basal Velocity Is Not Activated by N-Acetylthioureas.
[So] Source:PLoS One;11(1):e0146900, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lysine deacetylases (KDACs) are enzymes that reverse the post-translational modification of lysine acetylation. Recently, a series of N-acetylthioureas were synthesized and reported to enhance the activity of KDAC8 with a fluorogenic substrate. To determine if the activation was general, we synthesized three of the most potent N-acetylthioureas and measured their effect with peptide substrates and the fluorogenic substrate under multiple reaction conditions and utilizing two enzyme purification approaches. No activation was observed for any of the three N-acetylthioureas under any assayed conditions. Further characterization of KDAC8 kinetics with the fluorogenic substrate yielded a kcat/KM of 164 ± 17 in the absence of any N-acetylthioureas. This catalytic efficiency is comparable to or higher than that previously reported when KDAC8 was activated by the N-acetylthioureas, suggesting that the previously reported activation effect may be due to use of an enzyme preparation that contains a large fraction of inactive enzyme. Further characterization with a less active preparation and additional substrates leads us to conclude that N-acetylthioureas are not true activators of KDAC8 and only increase activity if the enzyme preparation is below the maximal basal activity.
[Mh] Termos MeSH primário: Histona Desacetilases/metabolismo
Proteínas Repressoras/metabolismo
Tioureia/análogos & derivados
[Mh] Termos MeSH secundário: Ensaios Enzimáticos
Fluorescamina/química
Histona Desacetilases/química
Histona Desacetilases/genética
Seres Humanos
Cinética
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/química
Proteínas Recombinantes/isolamento & purificação
Proteínas Repressoras/química
Proteínas Repressoras/genética
Especificidade por Substrato
Tioureia/síntese química
Tioureia/química
Tioureia/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Recombinant Proteins); 0 (Repressor Proteins); 38183-12-9 (Fluorescamine); 591-08-2 (acetylthiourea); EC 3.5.1.98 (HDAC8 protein, human); EC 3.5.1.98 (Histone Deacetylases); GYV9AM2QAG (Thiourea)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160109
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0146900


  6 / 489 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26716887
[Au] Autor:Omar MA; Hammad MA; Salman BI; Derayea SM
[Ad] Endereço:Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
[Ti] Título:Highly sensitive spectrofluorimetric method for determination of doxazosin through derivatization with fluorescamine; Application to content uniformity testing.
[So] Source:Spectrochim Acta A Mol Biomol Spectrosc;157:55-60, 2016 Mar 15.
[Is] ISSN:1873-3557
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A highly sensitive, simple and selective spectrofluorimetric method has been developed and validated for determination of doxazosin mesylate in pure form, pharmaceutical formulations and human plasma. The method is based on the reaction between doxazosin mesylate and fluorescamine in Teorell buffer solution (pH 3) to give highly fluorescent derivative that can be measured at 489 nm using excitation wavelength of 385 nm. Different experimental parameters affecting the reaction were carefully studied and optimized. The calibration plot was constructed over the concentration range of 16-400 ng mL(-1) with quantitation limit of 14.3 ng mL(-1). The developed procedure was validated according to ICH guidelines and the results were satisfactory. The proposed method has been successfully applied to the analysis of the cited drug in its pharmaceutical preparations as well as for content uniformity testing. The results showed excellent agreement with the reported method with respect to precision and accuracy. In addition, the drug concentration was determined in the spiked human plasma by the suggested method with % recovery in the range of 96.2-98.3% (SD; 0.76-0.93, n=5).
[Mh] Termos MeSH primário: Anti-Hipertensivos/análise
Anti-Hipertensivos/sangue
Doxazossina/análise
Doxazossina/sangue
Espectrometria de Fluorescência/métodos
[Mh] Termos MeSH secundário: Fluorescamina/química
Seres Humanos
Limite de Detecção
Preparações Farmacêuticas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Antihypertensive Agents); 0 (Pharmaceutical Preparations); 38183-12-9 (Fluorescamine); NW1291F1W8 (Doxazosin)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151231
[St] Status:MEDLINE


  7 / 489 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26402585
[Au] Autor:Toro TB; Watt TJ
[Ad] Endereço:Department of Chemistry, Xavier University of Louisiana, New Orleans, Louisiana, 70125.
[Ti] Título:KDAC8 substrate specificity quantified by a biologically relevant, label-free deacetylation assay.
[So] Source:Protein Sci;24(12):2020-32, 2015 Dec.
[Is] ISSN:1469-896X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Analysis of the human proteome has identified thousands of unique protein sequences that contain acetylated lysine residues in vivo. These modifications regulate a variety of biological processes and are reversed by the lysine deacetylase (KDAC) family of enzymes. Despite the known prevalence and importance of acetylation, the details of KDAC substrate recognition are not well understood. While several methods have been developed to monitor protein deacetylation, none are particularly suited for identifying enzyme-substrate pairs of label-free substrates across the entire family of lysine deacetylases. Here, we present a fluorescamine-based assay which is more biologically relevant than existing methods and amenable to probing substrate specificity. Using this assay, we evaluated the activity of KDAC8 and other lysine deacetylases, including a sirtuin, for several peptides derived from known acetylated proteins. KDAC8 showed clear preferences for some peptides over others, indicating that the residues immediately surrounding the acetylated lysine play an important role in substrate specificity. Steady-state kinetics suggest that the sequence surrounding the acetylated lysine affects binding affinity and catalytic rate independently. Our results provide direct evidence that potential KDAC8 substrates previously identified through cell based experiments can be directly deacetylated by KDAC8. Conversely, the data from this assay did not correlate well with predictions from previous screens for KDAC8 substrates using less biologically relevant substrates and assay conditions. Combining results from our assay with mass spectrometry-based experiments and cell-based experiments will allow the identification of specific KDAC-substrate pairs and lead to a better understanding of the biological consequences of these interactions.
[Mh] Termos MeSH primário: Histona Desacetilases/química
Histona Desacetilases/metabolismo
Lisina/química
Peptídeos/metabolismo
Mapeamento de Interação de Proteínas/métodos
Proteínas Repressoras/química
Proteínas Repressoras/metabolismo
[Mh] Termos MeSH secundário: Acetilação
Domínio Catalítico
Fluorescamina/química
Seres Humanos
Cinética
Modelos Moleculares
Peptídeos/química
Ligação Proteica
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Peptides); 0 (Repressor Proteins); 38183-12-9 (Fluorescamine); EC 3.5.1.98 (HDAC8 protein, human); EC 3.5.1.98 (Histone Deacetylases); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150925
[St] Status:MEDLINE
[do] DOI:10.1002/pro.2813


  8 / 489 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25587850
[Au] Autor:Ashby J; Duan Y; Ligans E; Tamsi M; Zhong W
[Ad] Endereço:Department of Chemistry, ‡Department of Biology, University of California, Riverside , Riverside, California 92521, United States.
[Ti] Título:High-throughput profiling of nanoparticle-protein interactions by fluorescamine labeling.
[So] Source:Anal Chem;87(4):2213-9, 2015 Feb 17.
[Is] ISSN:1520-6882
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A rapid, high throughput fluorescence assay was designed to screen interactions between proteins and nanoparticles. The assay employs fluorescamine, a primary-amine specific fluorogenic dye, to label proteins. Because fluorescamine could specifically target the surface amines on proteins, a conformational change of the protein upon interaction with nanoparticles will result in a change in fluorescence. In the present study, the assay was applied to test the interactions between a selection of proteins and nanoparticles made of polystyrene, silica, or iron oxide. The particles were also different in their hydrodynamic diameter, synthesis procedure, or surface modification. Significant labeling differences were detected when the same protein incubated with different particles. Principal component analysis (PCA) on the collected fluorescence profiles revealed clear grouping effects of the particles based on their properties. The results prove that fluorescamine labeling is capable of detecting protein-nanoparticle interactions, and the resulting fluorescence profile is sensitive to differences in nanoparticle's physical properties. The assay can be carried out in a high-throughput manner, and is rapid with low operation cost. Thus, it is well suited for evaluating interactions between a larger number of proteins and nanoparticles. Such assessment can help to improve our understanding on the molecular basis that governs the biological behaviors of nanomaterials. It will also be useful for initial examination of the bioactivity and reproducibility of nanomaterials employed in biomedical fields.
[Mh] Termos MeSH primário: Aminas/análise
Fluorescamina/química
Ensaios de Triagem em Larga Escala
Nanopartículas/química
Proteínas/química
[Mh] Termos MeSH secundário: Compostos Férricos/química
Indicadores e Reagentes/química
Poliestirenos/química
Análise de Componente Principal
Dióxido de Silício/química
Coloração e Rotulagem
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Amines); 0 (Ferric Compounds); 0 (Indicators and Reagents); 0 (Polystyrenes); 0 (Proteins); 1K09F3G675 (ferric oxide); 38183-12-9 (Fluorescamine); 7631-86-9 (Silicon Dioxide)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150115
[St] Status:MEDLINE
[do] DOI:10.1021/ac5036814


  9 / 489 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:24922589
[Au] Autor:Babu A; Wang Q; Muralidharan R; Shanker M; Munshi A; Ramesh R
[Ad] Endereço:Department of Pathology and ‡Peggy and Charles Stephenson Cancer Center, University of Oklahoma Health Sciences Center , Oklahoma City, Oklahoma 73104, United States.
[Ti] Título:Chitosan coated polylactic acid nanoparticle-mediated combinatorial delivery of cisplatin and siRNA/Plasmid DNA chemosensitizes cisplatin-resistant human ovarian cancer cells.
[So] Source:Mol Pharm;11(8):2720-33, 2014 Aug 04.
[Is] ISSN:1543-8392
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Development of resistance toward anticancer drugs results in ineffective therapy leading to increased mortality. Therefore, overriding resistance and restoring sensitivity to anticancer drugs will improve treatment efficacy and reduce mortality. While numerous mechanisms for drug resistance in cancer have previously been demonstrated, recent studies implicate a role for proteasome and the autophagy regulatory protein P62/SQSTM1 (P62) in contributing to drug resistance. Specifically, reduction in the expression of the ß5 subunit of the proteasome and/or enhanced P62 protein expression is known to contribute to cancer drug resistance such as cisplatin (CDDP) in ovarian cancer cells. Therefore, we hypothesized that restoration of ß5 expression and/or suppression of P62 protein expression in CDDP-resistant ovarian cancer cells will lead to restoration of sensitivity to CDDP and enhanced cell killing. To test our hypothesis we developed a biodegradable multifunctional nanoparticle (MNP) system that codelivered P62siRNA, ß5 plasmid DNA, and CDDP and tested its efficacy in CDDP resistant 2008/C13 ovarian cancer cells. MNP consisted of CDDP loaded polylactic acid nanoparticle as inner core and cationic chitosan (CS) consisting of ionically linked P62siRNA (siP62) and/or ß5 expressing plasmid DNA (pß5) as the outer layer. The MNPs were spherical in shape with a hydrodynamic diameter in the range of 280-350 nm, and demonstrated encapsulation efficiencies of 82% and 78.5% for CDDP and siRNA respectively. MNPs efficiently protected the siRNA and showed superior serum stability compared to naked siRNA as measured by gel retardation and spectrophotometry assays. The MNPs successfully delivered siP62 and pß5 to cause P62 knockdown and restoration of ß5 expression in 2008/C13 cells. Combined delivery of siP62, pß5, and CDDP using the MNPs resulted in a marked reduction in the IC50 value of CDDP in 2008/C13 cells from 125 ± 1.3 µM to 98 ± 0.6 µM (P < 0.05; 21.6% reduction) when compared to the reduction in the IC50 of CDDP observed in cells that had only siP62 delivered (IC50 = 106 ± 1.1 µM; P < 0.05; 15.2% reduction) or pß5 delivered (IC50 = 115 ± 2.8 µM; 8% reduction) via MNPs. Finally, our studies showed that the CDDP resistance index in 2008/C13 cells was reduced from 4.62 for free CDDP to 3.62 for MNP treatment. In conclusion our study results demonstrated the efficacy of our MNP in overcoming CDDP resistance in ovarian cancer cells.
[Mh] Termos MeSH primário: Quitosana/química
Cisplatino/administração & dosagem
Sistemas de Liberação de Medicamentos
Ácido Láctico/química
Nanopartículas/química
Neoplasias Ovarianas/tratamento farmacológico
Polímeros/química
[Mh] Termos MeSH secundário: Antineoplásicos/administração & dosagem
Linhagem Celular Tumoral
Sobrevivência Celular
Cisplatino/química
Resistência a Medicamentos Antineoplásicos
Feminino
Fluorescamina/química
Seres Humanos
Concentração Inibidora 50
Nanomedicina/métodos
Neoplasias Ovarianas/metabolismo
Tamanho da Partícula
Plasmídeos/metabolismo
Poliésteres
Complexo de Endopeptidases do Proteassoma/química
RNA Interferente Pequeno/metabolismo
Proteínas de Ligação a RNA/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (P62 protein, human); 0 (Polyesters); 0 (Polymers); 0 (RNA, Small Interfering); 0 (RNA-Binding Proteins); 33X04XA5AT (Lactic Acid); 38183-12-9 (Fluorescamine); 459TN2L5F5 (poly(lactide)); 9012-76-4 (Chitosan); EC 3.4.25.1 (Proteasome Endopeptidase Complex); Q20Q21Q62J (Cisplatin)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140613
[St] Status:MEDLINE
[do] DOI:10.1021/mp500259e


  10 / 489 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:24782430
[Au] Autor:Al Lawati HA; Al-Nadabi MM; Varma GB; Suliman FE; Al-Abri H
[Ad] Endereço:Department of Chemistry, College of Science, Sultan Qaboos University, PO Box 36, Al-Khod, 123, Oman.
[Ti] Título:A lab-on-a-chip device for analysis of amlodipine in biological fluids using peroxyoxalate chemiluminescence system.
[So] Source:Luminescence;29(8):1148-53, 2014 Dec.
[Is] ISSN:1522-7243
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A highly sensitive, rapid and economical method for the determination of amlodipine (AM) in biological fluids was developed using a peroxyoxalate chemiluminescence (CL) system in a lab-on-a-chip device. Peroxyoxalate-CL is an indirect type of CL that allows the detection of native fluorophores or compounds derivatized with fluorescent labels. Here, fluorescamine was reacted with AM, and the derivatization product was used in a bis-(2,4,6-trichlorophenyl)oxalate-CL system. Fluorescamine reacts selectively with aliphatic primary amine at neutral or basic pH. As most of the calcium channel blocker and many cardiovascular drugs do not contain primary amine, the developed method is highly selective. The parameters that influenced the CL signal intensity were studied carefully. These included the chip geometry, pH, concentration of reagents used and flow rates. Moreover, we confirmed our previous observation about the effects of imidazole, which is commonly used in the bis-(2,4,6-trichlorophenyl)oxalate-CL system as a catalyst, and found that the signal was significantly improved when imidazole was absent. Under optimized conditions, a calibration curve was obtained with a linear range (10-100 µg/L). The limit of detection was 3 µg/L, while the limit of quantification was 10 µg/L. Finally the method was applied for the determination of AM in biological fluids successfully.
[Mh] Termos MeSH primário: Anlodipino/análise
Dispositivos Lab-On-A-Chip
Medições Luminescentes/métodos
Oxalatos/química
[Mh] Termos MeSH secundário: Anlodipino/sangue
Calibragem
Desenho de Equipamento
Fluorescamina/química
Corantes Fluorescentes/química
Seres Humanos
Concentração de Íons de Hidrogênio
Limite de Detecção
Medições Luminescentes/instrumentação
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fluorescent Dyes); 0 (Oxalates); 1J444QC288 (Amlodipine); 38183-12-9 (Fluorescamine); 5796-84-9 (peroxyoxalate)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:141216
[Lr] Data última revisão:
141216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140501
[St] Status:MEDLINE
[do] DOI:10.1002/bio.2675



página 1 de 49 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde