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  1 / 2071 MEDLINE  
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[PMID]:29324346
[Au] Autor:Chen HJ; Zhou XB; Wang AL; Zheng BY; Yeh CK; Huang JD
[Ad] Endereço:College of Chemistry, State Key Laboratory of Photocatalysis on Energy and Environment, Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy, Fuzhou University, Fuzhou 350116, China.
[Ti] Título:Synthesis and biological characterization of novel rose bengal derivatives with improved amphiphilicity for sono-photodynamic therapy.
[So] Source:Eur J Med Chem;145:86-95, 2018 Feb 10.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Sono-Photodynamic therapy (SPDT) utilizing ultrasound and light has been demonstrated that this novel approach can lower dosage resulting in reduction of the potential side effects caused by sensitizers. Recently, a new formulation of rose bengal (RB) as an intralesional injection has completed clinical trials phase II for PDT treatment of melanoma cancer. However, the inherent unfavorable pharmacological properties of RB hindered its extensive clinical development. With the aim to identify new RB derivatives (RBDs) with enhanced photodynamic and sonodynamic anticancer efficiency, a series of amphiphilic RBDs have been designed, synthesized and biological characterized. Among them, RBD4 significantly improved cellular uptake and enhanced intracellular ROS generation efficiency upon light and ultrasound irradiation, resulting in dramatically improved anticancer potency. Notably, RBD4 has a relative potency similar to sinoporphyrin sodium (DVDMS), indicating its further potential application for SPDT.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Fotoquimioterapia
Rosa Bengala/farmacologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/síntese química
Antineoplásicos/química
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Células Hep G2
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Camundongos
Estrutura Molecular
Espécies Reativas de Oxigênio/análise
Espécies Reativas de Oxigênio/metabolismo
Rosa Bengala/síntese química
Rosa Bengala/química
Relação Estrutura-Atividade
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Reactive Oxygen Species); 1ZPG1ELY14 (Rose Bengal)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE


  2 / 2071 MEDLINE  
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[PMID]:29353702
[Au] Autor:Manoil D; Lange N; Bouillaguet S
[Ad] Endereço:Endodontics Unit, Section of Dental Medicine, University of Geneva, 1, rue Michel-Servet, CH-1206 Geneva, Switzerland. Electronic address: Daniel.Manoil@unige.ch.
[Ti] Título:Enzyme-mediated photoinactivation of Enterococcus faecalis using Rose Bengal-acetate.
[So] Source:J Photochem Photobiol B;179:84-90, 2018 Feb.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Rose Bengal-acetate (RB-Ac) is a pro-photosensitizer claimed to diffuse into target cells, where the acetate groups are hydrolyzed and the photosensitizing properties of Rose Bengal (RB) are restored. Despite promising results on tumor cells, the interaction of RB-Ac with bacteria has never been investigated. This study aimed to assess the interaction of RB-Ac with Enterococcus faecalis and to evaluate its potential use in antimicrobial photodynamic therapy (aPDT). Spectrofluorometry was used to assess the ability of E. faecalis to hydrolyze the RB-Ac compound. Fluorescence microscopy was employed to observe the distribution and to evaluate the cellular uptake of the RB produced. The antibacterial efficiency of RB-Ac-mediated aPDT was assessed by flow cytometry in combination with the LIVE/DEAD® staining. Results showed that RB-Ac was successfully hydrolyzed in the presence of E. faecalis cells. The RB produced appeared to incorporate the membrane of bacteria. Higher concentrations of RB-Ac resulted in higher incorporation of RB. The blue-light irradiation of RB-Ac-treated samples significantly reduced bacterial viability. Less than 0.01% of E. faecalis survived after incubation with 200 µM RB-Ac during 900 min and blue-light activation. The current report indicates that E. faecalis cells can hydrolyze the RB-Ac compound to produce active RB. The use of RB-Ac did not appear to allow cytoplasmic internalization of the RB produced, which rather incorporated the membrane bilayers of E. faecalis. The use of RB-Ac did not provide additional advantages over RB in terms of PS localization. Nonetheless, sufficient RB was produced and incorporated into the membranes of bacteria to elicit effective aPDT.
[Mh] Termos MeSH primário: Enterococcus faecalis/efeitos dos fármacos
Fármacos Fotossensibilizantes/farmacologia
Rosa Bengala/análogos & derivados
[Mh] Termos MeSH secundário: Enterococcus faecalis/efeitos da radiação
Hidrólise/efeitos da radiação
Luz
Viabilidade Microbiana/efeitos dos fármacos
Viabilidade Microbiana/efeitos da radiação
Microscopia de Fluorescência
Rosa Bengala/farmacologia
Espectrometria de Fluorescência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosensitizing Agents); 0 (Rose Bengal acetate); 1ZPG1ELY14 (Rose Bengal)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180123
[St] Status:MEDLINE


  3 / 2071 MEDLINE  
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[PMID]:29242903
[Au] Autor:Seiler TG; Engler M; Beck E; Birngruber R; Kochevar IE
[Ad] Endereço:Wellman Center for Photomedicine-Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States.
[Ti] Título:Interface Bonding With Corneal Crosslinking (CXL) After LASIK Ex Vivo.
[So] Source:Invest Ophthalmol Vis Sci;58(14):6292-6298, 2017 Dec 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: Interface bonding with corneal crosslinking (CXL) after LASIK using two different photosensitizers was studied ex vivo. Methods: A LASIK flap was created in enucleated rabbit eyes using a femtosecond laser. After the dissection, CXL was performed to seal the interface. In one group interface CXL was performed using rose bengal and green light, whereas in a second group riboflavin and UV-A light was used. In both groups irradiance, radiant exposure, dye concentration, and imbibition time was varied. In a control group, LASIK only was performed. After the procedures, the maximal shear-force required to separate the flap from the stroma was measured. Additionally, corneal transmission spectra were recorded. Results: Optimized parameters for rose bengal/green-light bonding lead to a 2.1-fold increase in shear-force compared with untreated control eyes (P < 0.01). The optimal parameter combination was: irradiance of 180 mW/cm2 for 14 minutes (total radiant exposure 150 J/cm2), rose bengal concentration 0.1%, and an imbibition time of 2 minutes. Optimized riboflavin/UV-A light parameters were 0.5% for 2 minutes with a radiant exposure of 8.1 J/cm2 obtained by an irradiance of 30 mW/cm2 for 4.5 minutes. These optimized parameters lead to a 2-fold increase compared with untreated control eyes (P < 0.01). Optical transmission experiments suggest safety for more posterior structures. Conclusions: Based on ex-vivo results, interface bonding after LASIK using crosslinking with either rose bengal or riboflavin increases the adhesion between flap and stromal bed. In vivo trials are needed to evaluate the temporal evolution of the effect.
[Mh] Termos MeSH primário: Colágeno/uso terapêutico
Substância Própria/efeitos dos fármacos
Reagentes para Ligações Cruzadas/farmacologia
Ceratomileuse Assistida por Excimer Laser In Situ
Fotoquimioterapia/métodos
Fármacos Fotossensibilizantes/uso terapêutico
Complicações Pós-Operatórias/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Substância Própria/patologia
Substância Própria/cirurgia
Modelos Animais de Doenças
Lasers de Excimer/uso terapêutico
Miopia/fisiopatologia
Miopia/cirurgia
Período Pós-Operatório
Coelhos
Refração Ocular
Riboflavina/uso terapêutico
Rosa Bengala
Retalhos Cirúrgicos
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 0 (Photosensitizing Agents); 1ZPG1ELY14 (Rose Bengal); 9007-34-5 (Collagen); TLM2976OFR (Riboflavin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-22426


  4 / 2071 MEDLINE  
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[PMID]:28700779
[Au] Autor:Gallego-Muñoz P; Ibares-Frías L; Lorenzo E; Marcos S; Peréz-Merino P; Bekesi N; Kochevar IE; Martínez-García MC
[Ad] Endereço:Departamento de Biología Celular, Histología y Farmacología, GIR de Técnicas Ópticas para el Diagnóstico, Universidad de Valladolid, Valladolid, Spain.
[Ti] Título:Corneal Wound Repair After Rose Bengal and Green Light Crosslinking: Clinical and Histologic Study.
[So] Source:Invest Ophthalmol Vis Sci;58(9):3471-3480, 2017 Jul 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: To evaluate corneal wound healing after treatment with a new collagen crosslinking protocol using rose bengal dye and green light (RGX). Methods: One cornea of 20 New Zealand rabbits was de-epithelialized (DE) in an 8-mm diameter circle and, in another group (n = 25), the DE corneas were then stained with 0.1% rose bengal for 2 minutes and exposed to green light (532 nm) for 7 minutes (RGX). The contralateral eyes without treatment acted as controls. The animals were clinically followed including fluorescein staining and pachymetry. Healing events were analyzed after euthanasia at 2, 30, and 60 days. Cell death (TUNEL assay), cell proliferation (5-bromo-2'-deoxyuridine incorporation), and cell differentiation to myofibroblasts (α-SMA labeling) were carried out. In addition, loss of keratocytes and subsequent repopulation of the corneal stroma were quantified on hematoxylin-eosin-stained sections. Results: Wound closure was slower after RGX (4.4 days) then after DE (3.3 days). Cell death was restricted to the anterior central stroma, and the cellular decrease did not differ significantly between RGX and DE corneas. Cell proliferation in the epithelium and stroma appeared at 2 days. In both DE and RGX corneas, recovery of the epithelium was complete at day 30, although cell repopulation of the stroma was not complete at 60 days. Conclusions: The healing response in corneas after RGX is very similar to that observed after DE alone, suggesting that, along with its short treatment time and limited effect on keratocytes, RGX displays good potential for clinical cornea stiffening.
[Mh] Termos MeSH primário: Colágeno/farmacologia
Córnea/patologia
Lesões da Córnea/tratamento farmacológico
Reagentes para Ligações Cruzadas/farmacologia
Luz
Rosa Bengala/farmacologia
Cicatrização/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Córnea/efeitos dos fármacos
Modelos Animais de Doenças
Feminino
Corantes Fluorescentes/farmacologia
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 0 (Fluorescent Dyes); 1ZPG1ELY14 (Rose Bengal); 9007-34-5 (Collagen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.16-21365


  5 / 2071 MEDLINE  
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[PMID]:28614411
[Au] Autor:Minol JP; Reinsch I; Luik M; Leferink A; Barth M; Assmann A; Lichtenberg A; Akhyari P
[Ad] Endereço:Department of Cardiovascular Surgery, University Hospital, Dusseldorf, Germany.
[Ti] Título:Focal induction of ROS-release to trigger local vascular degeneration.
[So] Source:PLoS One;12(6):e0179342, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reactive oxygen species (ROS) play an important role in the process of cardiovascular degeneration. We evaluated the potential of a controlled, local induction of ROS-release by application of rose bengal (RB) and photo energy to induce atherosclerosis-like focal vascular degeneration in vivo. After injection of RB, rats fed with a pro-degenerative diet underwent focal irradiation of the abdominal aorta by a green laser (ROS group), while the controls received irradiation without RB. Aortic tissue was analyzed by histology and immunohistochemistry at 0, 2, 4, 8, 28 and 56 days (n = 5). The intimal surface topography was analyzed by scanning electron microscopy. In the ROS group, an initial thrombus formation had disappeared by day 8. Similarly, ROS-derived products displayed the highest concentrations at day 0. Relative matrix metalloproteinase (MMP) activity achieved a maximum after 8 days (ROS group vs. CONTROL GROUP: 1.60 ± 0.11 vs. 0.98 ± 0.01; p < 0.001). After 28 days, no significant differences in any aspect were found between the ROS group and the controls. However, after 56 days, the aortic tissue of ROS animals exhibited relative media-pronounced thickening (ROS vs. CONTROL: 2.15 ± 0.19 vs. 0.87 ± 0.10; p < 0.001) with focal calcification and reduced expression of alpha smooth muscle actin (aSMA). The ROS-releasing application of RB and photo energy allowed for the induction of vascular degeneration in a rodent model. This protocol may be used for the focal induction of vascular disease without systemic side effects and can thereby elucidate the role of ROS in the multifactorial processes of vessel degeneration and atherogenesis.
[Mh] Termos MeSH primário: Aorta Abdominal/metabolismo
Dieta
Espécies Reativas de Oxigênio/metabolismo
Doenças Vasculares/metabolismo
[Mh] Termos MeSH secundário: Actinas/metabolismo
Animais
Aorta Abdominal/patologia
Aorta Abdominal/efeitos da radiação
Cálcio/sangue
Caspase 3/metabolismo
Colesterol/sangue
Imuno-Histoquímica
Lasers
Masculino
Metaloproteinases da Matriz/metabolismo
Microscopia Eletrônica de Varredura
Músculo Liso/química
Fosfatos/sangue
Ratos Wistar
Espécies Reativas de Oxigênio/química
Rosa Bengala/química
Triglicerídeos/sangue
Túnica Íntima/metabolismo
Túnica Íntima/efeitos da radiação
Túnica Íntima/ultraestrutura
Doenças Vasculares/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Phosphates); 0 (Reactive Oxygen Species); 0 (Triglycerides); 1ZPG1ELY14 (Rose Bengal); 97C5T2UQ7J (Cholesterol); EC 3.4.22.- (Caspase 3); EC 3.4.24.- (Matrix Metalloproteinases); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179342


  6 / 2071 MEDLINE  
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[PMID]:28442903
[Au] Autor:Zhan J; Ma Z; Wang D; Li X; Li X; Le L; Kang A; Hu P; She L; Yang F
[Ad] Endereço:Department of Pharmacy, Hebei North University, Zhangjiakou, Hebei.
[Ti] Título:Magnetic and pH dual-responsive mesoporous silica nanocomposites for effective and low-toxic photodynamic therapy.
[So] Source:Int J Nanomedicine;12:2733-2748, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Nonspecific targeting, large doses and phototoxicity severely hamper the clinical effect of photodynamic therapy (PDT). In this work, superparamagnetic Fe O mesoporous silica nanoparticles grafted by pH-responsive block polymer polyethylene glycol- -poly(aspartic acid) (PEG- -PAsp) were fabricated to load the model photosensitizer rose bengal (RB) in the aim of enhancing the efficiency of PDT. Compared to free RB, the nanocomposites (polyethylene glycol- -polyaspartate-modified rose bengal-loaded magnetic mesoporous silica [RB-MMSNs]) could greatly enhance the cellular uptake due to their effective endocytosis by mouse melanoma B16 cell and exhibited higher induced apoptosis although with little dark toxicity. RB-MMSNs had little dark toxicity and even much could be facilitated by magnetic field in vitro. RB-MMSNs demonstrated 10 times induced apoptosis efficiency than that of free RB at the same RB concentration, both by cell counting kit-8 (CCK-8) result and apoptosis detection. Furthermore, RB-MMSNs-mediated PDT in vivo on tumor-bearing mice showed steady physical targeting of RB-MMSNs to the tumor site; tumor volumes were significantly reduced in the magnetic field with green light irradiation. More importantly, the survival time of tumor-bearing mice treated with RB-MMSNs was much prolonged. Henceforth, polyethylene glycol- -polyaspartate-modified magnetic mesoporous silica (MMSNs) probably have great potential in clinical cancer photodynamic treatment because of their effective and low-toxic performance as photosensitizers' vesicles.
[Mh] Termos MeSH primário: Nanocompostos/química
Nanocompostos/uso terapêutico
Fotoquimioterapia/métodos
Fármacos Fotossensibilizantes/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Linhagem Celular Tumoral
Endocitose/efeitos dos fármacos
Óxido Ferroso-Férrico/química
Concentração de Íons de Hidrogênio
Magnetismo
Masculino
Melanoma Experimental/tratamento farmacológico
Melanoma Experimental/patologia
Camundongos
Camundongos Endogâmicos C57BL
Nanopartículas/química
Fármacos Fotossensibilizantes/química
Fármacos Fotossensibilizantes/farmacologia
Polietilenoglicóis/química
Rosa Bengala/administração & dosagem
Rosa Bengala/análogos & derivados
Rosa Bengala/química
Dióxido de Silício/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosensitizing Agents); 0 (polyethylene glycol-block-polyaspartic acid); 1ZPG1ELY14 (Rose Bengal); 30IQX730WE (Polyethylene Glycols); 4159-77-7 (Rose Bengal B); 7631-86-9 (Silicon Dioxide); XM0M87F357 (Ferrosoferric Oxide)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S127528


  7 / 2071 MEDLINE  
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[PMID]:28297026
[Au] Autor:Bekesi N; Gallego-Muñoz P; Ibarés-Frías L; Perez-Merino P; Martinez-Garcia MC; Kochevar IE; Marcos S
[Ad] Endereço:Instituto de Optica, Consejo Superior de Investigaciones Cientificas, Madrid, Spain.
[Ti] Título:Biomechanical Changes After In Vivo Collagen Cross-Linking With Rose Bengal-Green Light and Riboflavin-UVA.
[So] Source:Invest Ophthalmol Vis Sci;58(3):1612-1620, 2017 Mar 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: To compare corneal biomechanical properties after in vivo and ex vivo cross-linking (CXL) using rose bengal-green light (RGX) or riboflavin-UVA (UVX). Methods: Corneas of 30 rabbits were treated in vivo by the two CXL modalities monolaterally (Group 1) or bilaterally (Group 2). Rabbits in Group 1 were euthanized 1 month after treatments and in Group 2 two months after treatment. Ex vivo CXL was also performed. Eyes were measured by Scheimpflug air puff corneal deformation imaging (Corvis ST) under constant IOP. Corneal deformation parameters were assessed. Inherent corneal biomechanical properties were estimated using inverse finite element modeling. Results: Peak to peak distance decreased 16% 2 months after RGX, and 4% and 20% 1 and 2 months after UVX, respectively. The equivalent Young's modulus (Eeq) increased relative to the control during the post treatment period for both RGX and UVX. The Eeq increased by factors of 3.4 (RGX) and 1.7 (UVX) 1 month and by factors of 10.7 (RGX) and 7.3 (UVX) 2 months after treatment. However, the Eeq values for ex vivo CXL were much greater than produced in vivo. The ex vivo Eeq was greater than the 1-month in vivo values by factors of 8.1 (RGX) and 9.1 (UVX) and compared with 2 month by factors of 2.5 (RGX) and 2.1 (UVX). Conclusions: These results indicate that corneal stiffness increases after CXL, and further increases as a function of time after both RGX and UVX. Also, while biomechanical properties determined after ex vivo CXL are indicative of corneal stiffening, they may not provide entirely accurate information about the responses to CXL in vivo.
[Mh] Termos MeSH primário: Colágeno/farmacologia
Doenças da Córnea/tratamento farmacológico
Substância Própria/fisiopatologia
Reagentes para Ligações Cruzadas/farmacologia
Riboflavina/farmacologia
Rosa Bengala/farmacologia
Raios Ultravioleta
[Mh] Termos MeSH secundário: Animais
Doenças da Córnea/patologia
Doenças da Córnea/fisiopatologia
Substância Própria/efeitos dos fármacos
Substância Própria/patologia
Modelos Animais de Doenças
Elasticidade
Corantes Fluorescentes/farmacologia
Fármacos Fotossensibilizantes/farmacologia
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 0 (Fluorescent Dyes); 0 (Photosensitizing Agents); 1ZPG1ELY14 (Rose Bengal); 9007-34-5 (Collagen); TLM2976OFR (Riboflavin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-21475


  8 / 2071 MEDLINE  
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[PMID]:28281771
[Au] Autor:Chisi SL; Marageni Y; Naidoo P; Zulu G; Akol GW; Van Heerden H
[Ad] Endereço:Department of Agriculture and Rural Development, Allerton Provincial Veterinary Laboratory. songechisi@gmail.com.
[Ti] Título:An evaluation of serological tests in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province in South Africa.
[So] Source:J S Afr Vet Assoc;88(0):e1-e7, 2017 Feb 28.
[Is] ISSN:2224-9435
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:The diagnostic sensitivity (DSe) of the Rose Bengal test (RBT), the complement fixation test (CFT), the serum agglutination test (SAT), the competitive enzyme-linked immunosorbent assay (cELISA) and the indirect ELISA (iELISA) were determined in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture (gold standard). Natural brucellosis infection status of animals was determined by culturing and identification of Brucella abortus biovar 1 from abomasal fluid, milk, hygroma fluid, lymph nodes or uterine discharges samples. The diagnostic specificity (DSp) of the tests mentioned above was determined using samples from known negative herds. There was no statistically significant difference between the tests in their ability to diagnose brucellosis. The RBT and iELISA had the highest DSe of 95.8%, whereas RBT and CFT had the highest DSp of 100%. In South African laboratories, the RBT and CFT serological tests are used, because of the cost efficacy of CFT when compared to the less labour intensive but more expensive iELISA.
[Mh] Termos MeSH primário: Testes de Aglutinação/veterinária
Brucella abortus/isolamento & purificação
Brucelose Bovina/diagnóstico
Testes de Fixação de Complemento/veterinária
Ensaio de Imunoadsorção Enzimática/veterinária
[Mh] Termos MeSH secundário: Testes de Aglutinação/normas
Animais
Brucelose Bovina/sangue
Bovinos
Testes de Fixação de Complemento/normas
Ensaio de Imunoadsorção Enzimática/normas
Linfonodos/microbiologia
Leite/microbiologia
Rosa Bengala
Sensibilidade e Especificidade
África do Sul
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
1ZPG1ELY14 (Rose Bengal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.4102/jsava.v88i0.1381


  9 / 2071 MEDLINE  
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[PMID]:28254338
[Au] Autor:Hou B; Yang W; Dong C; Zheng B; Zhang Y; Wu J; Wang H; Chang J
[Ad] Endereço:School of Life Sciences, Tianjin University, PR China.
[Ti] Título:Controlled co-release of doxorubicin and reactive oxygen species for synergistic therapy by NIR remote-triggered nanoimpellers.
[So] Source:Mater Sci Eng C Mater Biol Appl;74:94-102, 2017 May 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:How to encapsulate and transport the payload of multiple therapeutic compounds avoiding premature leakage, and simultaneously co-release them rapidly at specific lesions still remains the major concern in clinic. Herein, we designed the UCN@mSiO -(Azo+RB) (azobenzene groups and Rose Bengal) nanoimpellers, which used the multicolor-emission capability of the core-shell upconverting nanoparticles (UCNs) at a single excitation wavelength to co-release anticarcinogen doxorubicin (Dox) and reactive oxygen species (ROS) for combined chemotherapy and photodynamic therapy (PDT). The nanoimpeller was formed from UCN inner core, mesoporous silica shell, and light triggers Azo and RB molecules. The UCNs emitting UV/blue and green/red multiband light were used to activate the photoresponsive Azo and photosensitizer RB molecules; The mesoporous silica shell offered the possibilities to load anticancer drug and conjugate the light triggers; As there are strong charge interaction and hydrogen bonds between Dox and surface silanols of mesoporous silica, the azobenzene molecules worked as "gatekeeper" and "molecular stirrer" to precisely trap and propel the release of Dox under the external stimuli. The time-dependent drug release analysis, ROS production test and PDT test suggested that the nanoparticles may serve as a useful multifunctional nanoplatform for synergistic therapy and cancer diagnostic.
[Mh] Termos MeSH primário: Doxorrubicina/química
Portadores de Fármacos/química
Raios Infravermelhos
Nanopartículas/química
Espécies Reativas de Oxigênio/metabolismo
[Mh] Termos MeSH secundário: Antibióticos Antineoplásicos/química
Antibióticos Antineoplásicos/metabolismo
Antibióticos Antineoplásicos/toxicidade
Compostos Azo/química
Sobrevivência Celular/efeitos dos fármacos
Doxorrubicina/metabolismo
Doxorrubicina/toxicidade
Portadores de Fármacos/efeitos da radiação
Liberação Controlada de Fármacos
Sinergismo Farmacológico
Células HeLa
Seres Humanos
Nanopartículas/efeitos da radiação
Nanopartículas/toxicidade
Fármacos Fotossensibilizantes/química
Rosa Bengala/química
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibiotics, Antineoplastic); 0 (Azo Compounds); 0 (Drug Carriers); 0 (Photosensitizing Agents); 0 (Reactive Oxygen Species); 1ZPG1ELY14 (Rose Bengal); 80168379AG (Doxorubicin); F0U1H6UG5C (azobenzene)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170614
[Lr] Data última revisão:
170614
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE


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[PMID]:28230241
[Au] Autor:Foote M; Read T; Thomas J; Wagels M; Burmeister B; Smithers BM
[Ad] Endereço:Department of Radiation Oncology, Princess Alexandra Hospital, Queensland Health, Brisbane, Australia.
[Ti] Título:Results of a phase II, open-label, non-comparative study of intralesional PV-10 followed by radiotherapy for the treatment of in-transit or metastatic melanoma.
[So] Source:J Surg Oncol;115(7):891-897, 2017 Jun.
[Is] ISSN:1096-9098
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In-transit and recurrent dermal or subcutaneous melanoma metastases represent a significant burden of advanced disease. Intralesional Rose Bengal can elicit tumor selective ablation and a T-cell mediated abscopal effect in untreated lesions. A subset of patients in a phase II trial setting received external beam radiotherapy to their recurrent lesions with complete or partial response and no significant acute radiation reaction. METHODS: An open-label, single-arm phase II study was performed to assess the efficacy and safety of PV-10 followed by hypofractionated radiotherapy. Patients had in-transit melanoma metastases suitable for IL therapy and radiotherapy. RESULTS: Fifteen patients were enrolled and thirteen completed both treatment components. The overall response rate was 86.6% and the clinical benefit was 93.3% on an intention to treat analysis (CR 33.3%, PR 53.3%, SD 6.7%). The median follow up duration was 19.25 months. Size of metastases (<10 mm) predicted lesion complete response (74.6%). Treatment was well tolerated with no associated grade 4 or 5 adverse events. CONCLUSIONS: The combination of PV-10 and radiotherapy resulted in lesion-specific, normal tissue-sparing, ablation of disease with minimal local or systemic adverse effects.
[Mh] Termos MeSH primário: Corantes Fluorescentes/uso terapêutico
Melanoma/terapia
Radioterapia Adjuvante
Rosa Bengala/uso terapêutico
Neoplasias Cutâneas/terapia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Radioterapia Hipofracionada
Feminino
Seres Humanos
Injeções Intralesionais
Masculino
Melanoma/mortalidade
Melanoma/secundário
Meia-Idade
Recidiva Local de Neoplasia
Neoplasias Cutâneas/mortalidade
Neoplasias Cutâneas/secundário
[Pt] Tipo de publicação:CLINICAL TRIAL, PHASE II; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fluorescent Dyes); 1ZPG1ELY14 (Rose Bengal)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170823
[Lr] Data última revisão:
170823
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170224
[St] Status:MEDLINE
[do] DOI:10.1002/jso.24580



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