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[PMID]: | 29216958 |
[Au] Autor: | Guesmi F; Ben Hadj AS; Landoulsi A |
[Ad] Endereço: | Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences of Bizerte, University of Carthage, Zarzouna 7021, Tunisia. |
[Ti] Título: | Investigation of Extracts from Tunisian Ethnomedicinal Plants as Antioxidants, Cytotoxins, and Antimicrobials. |
[So] Source: | Biomed Environ Sci;30(11):811-824, 2017 Nov. | [Is] ISSN: | 0895-3988 |
[Cp] País de publicação: | Netherlands |
[La] Idioma: | eng |
[Ab] Resumo: | OBJECTIVE: To determine the medicinal potential of various plants and their parts extracted with different solvents. METHODS: The total phenolic content of acetonitrile/water (60%-40%) (ACN/W) and aqueous (W) extract fractions was determined by high-performance liquid chromatography (HPLC), and terpenic compounds were detected by gas chromatography/mass spectrometry (GC/MS). Antioxidant activity of the samples was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and ß-carotene bleaching method. Cell viability was investigated by thiazolyl blue tetrazolium bromide [3-(4,5-dimethylthiazol)-2-yl 2,5-diphenyltetrazolium bromide] (MTT) assay. The mechanisms involved in cytotoxic activity were investigated in a murine macrophage cell line (RAW 264.7) and cancer lines. RESULTS: Our findings show that 11 plant species exhibited biological activity. In addition, moderate antibacterial activity was reported against one or more of the tested bacterial strains at two concentrations: 300 µg and 3 mg/disc. Furthermore, our data reveal that among all plants investigated, some extract and hydrophobic fractions were potent scavengers of the DPPH radical (6.78 µg/mL < EC50 < 8.55 µg/mL). Taken together, our results show that Nerium oleander (NOACN/W) and Pituranthos tortuosus (PTACN/W) were highly cytotoxic against RAW 264.7 cells with IC80 values of 0.36, and 1.55 µg/mL, respectively. In contrast, murine macrophage cell lines had low growth and were significantly sensitive to water extracts of Thymus hirtus sp. algeriensis (THW), Lavandula multifida (LMW), and ACN/W extract of Erica multiflora (EMACN/W) at doses > 400, 47.20, and 116.74 µg/mL, respectively. The current work demonstrates that RAW 264.7 cell proliferation was inhibited by samples in a dose-dependent manner. CONCLUSION: Our findings, validated through free radical scavenging activity, agar diffusion assay, and cytotoxicity of essential oils towards cancer cells, show that ethnomedicinal plants used in this work have a novel application as a tumor suppressor. |
[Mh] Termos MeSH primário: |
Antibacterianos/farmacologia Antineoplásicos Fitogênicos/farmacologia Citotoxinas/farmacologia Extratos Vegetais/farmacologia Plantas Medicinais/química
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[Mh] Termos MeSH secundário: |
Animais Antibacterianos/química Antineoplásicos Fitogênicos/química Bactérias/efeitos dos fármacos Compostos de Bifenilo Linhagem Celular Citotoxinas/química Etnobotânica Camundongos Estrutura Molecular Fenóis/química Fenóis/farmacologia Picratos Extratos Vegetais/química Terpenos/química Terpenos/farmacologia Tunísia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Anti-Bacterial Agents); 0 (Antineoplastic Agents, Phytogenic); 0 (Biphenyl Compounds); 0 (Cytotoxins); 0 (Phenols); 0 (Picrates); 0 (Plant Extracts); 0 (Terpenes); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl) |
[Em] Mês de entrada: | 1803 |
[Cu] Atualização por classe: | 180305 |
[Lr] Data última revisão:
| 180305 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 171209 |
[St] Status: | MEDLINE |
[do] DOI: | 10.3967/bes2017.109 |
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