Base de dados : MEDLINE
Pesquisa : D02.455.849.291 [Categoria DeCS]
Referências encontradas : 12301 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 1231 ir para página                         

  1 / 12301 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29320813
[Au] Autor:Muñoz-Carrillo JL; Muñoz-López JL; Muñoz-Escobedo JJ; Maldonado-Tapia C; Gutiérrez-Coronado O; Contreras-Cordero JF; Moreno-García MA
[Ad] Endereço:Laboratory of Cell Biology and Microbiology, Academic Unit of Biological Sciences, Autonomous University of Zacatecas, Zacatecas, Zacatecas, México.
[Ti] Título:Therapeutic Effects of Resiniferatoxin Related with Immunological Responses for Intestinal Inflammation in Trichinellosis.
[So] Source:Korean J Parasitol;55(6):587-599, 2017 Dec.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:The immune response against Trichinella spiralis at the intestinal level depends on the CD4+ T cells, which can both suppress or promote the inflammatory response through the synthesis of diverse cytokines. During the intestinal phase, the immune response is mixed (Th1/Th2) with the initial predominance of the Th1 response and the subsequent domination of Th2 response, which favor the development of intestinal pathology. In this context, the glucocorticoids (GC) are the pharmacotherapy for the intestinal inflammatory response in trichinellosis. However, its therapeutic use is limited, since studies have shown that treatment with GC suppresses the host immune system, favoring T. spiralis infection. In the search for novel pharmacological strategies that inhibit the Th1 immune response (proinflammatory) and assist the host against T. spiralis infection, recent studies showed that resiniferatoxin (RTX) had anti-inflammatory activity, which decreased the serum levels of IL-12, INF-γ, IL-1ß, TNF-α, NO, and PGE2, as well the number of eosinophils in the blood, associated with decreased intestinal pathology and muscle parasite burden. These researches demonstrate that RTX is capable to inhibit the production of Th1 cytokines, contributing to the defense against T. spiralis infection, which places it as a new potential drug modulator of the immune response.
[Mh] Termos MeSH primário: Diterpenos/farmacologia
Diterpenos/uso terapêutico
Enteropatias Parasitárias/tratamento farmacológico
Enteropatias Parasitárias/imunologia
Intestinos/imunologia
Triquinelose/tratamento farmacológico
Triquinelose/imunologia
[Mh] Termos MeSH secundário: Animais
Linfócitos T CD4-Positivos/imunologia
Citocinas/metabolismo
Eosinófilos/imunologia
Seres Humanos
Mediadores da Inflamação/metabolismo
Contagem de Leucócitos
Células Th1/imunologia
Células Th2/imunologia
Trichinella spiralis/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cytokines); 0 (Diterpenes); 0 (Inflammation Mediators); A5O6P1UL4I (resiniferatoxin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2017.55.6.587


  2 / 12301 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29441915
[Au] Autor:Cao L-; Yan M; Ma YX; Zhang BK; Fang PF; Xiang DX; Li ZH; Gong H; Deng Y; Li HD
[Ti] Título:Isoliquiritigenin protects against triptolide-induced hepatotoxicity in mice through Nrf2 activation.
[So] Source:Pharmazie;71(7):394-397, 2016 Jul 07.
[Is] ISSN:0031-7144
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Isoliquiritigenin, a flavonoid found in licorice, has been considered as an antioxidive and hepato-protective agent. Recent studies have shown that a possible mechanism for triptolide-induced hepatotoxicity is related to oxidative damage induced by reactive oxygen species. This study was done to investigate the protection effect of isoliquiritigenin against triptolide-induced hepatotoxicity and the mechanism involved. An acute liver injury model was established by intraperitoneal injection of triptolide (1.0 mg · kg-1) in mice. Different doses of isoliquiritigenin (12.5, 25 and 50 mg · kg-1) were employed as protection. The activities of AST, ALT, ALP and LDH in serum and levels of GSH, GPx, SOD, CAT and MDA in liver tissue were detected. The histopathological changes of liver tissues were observed after HE staining. The protein expression of Nrf2 was detected by western blot. Pretreatment with isoliquiritigenin significantly prevented the triptolide-induced hepatotoxicity indicated by reduced activities of AST, ALT, ALP and LDH. Moreover, isoliquiritigenin pretreatment also prevented from triptolide-induced hepatotoxicity by inhibiting MDA and restoring the levels of GSH, GPx, SOD and CAT. In addition, isoliquiritigenin could attenuate histopathological changes induced by triptolide. Furthermore, the results indicated that isoliquiritigenin pretreatment caused an increase in the protein expression of Nrf2. These results indicated that isoliquiritigenin could protect against triptolide-induced hepatotoxicity via activation of the Nrf2 pathway.
[Mh] Termos MeSH primário: Chalconas/farmacologia
Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle
Diterpenos/antagonistas & inibidores
Diterpenos/toxicidade
Fator 2 Relacionado a NF-E2/metabolismo
Fenantrenos/antagonistas & inibidores
Fenantrenos/toxicidade
Substâncias Protetoras/farmacologia
[Mh] Termos MeSH secundário: Animais
Doença Hepática Induzida por Substâncias e Drogas/patologia
Compostos de Epóxi/antagonistas & inibidores
Compostos de Epóxi/toxicidade
Fígado/efeitos dos fármacos
Fígado/metabolismo
Testes de Função Hepática
Masculino
Malondialdeído/antagonistas & inibidores
Camundongos
Camundongos Endogâmicos ICR
Fator 2 Relacionado a NF-E2/efeitos dos fármacos
Estresse Oxidativo/efeitos dos fármacos
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chalcones); 0 (Diterpenes); 0 (Epoxy Compounds); 0 (NF-E2-Related Factor 2); 0 (Nfe2l2 protein, mouse); 0 (Phenanthrenes); 0 (Protective Agents); 19ALD1S53J (triptolide); 4Y8F71G49Q (Malondialdehyde); B9CTI9GB8F (isoliquiritigenin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1691/ph.2016.6535


  3 / 12301 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28460475
[Au] Autor:Song JM; Molla K; Anandharaj A; Cornax I; O Sullivan MG; Kirtane AR; Panyam J; Kassie F
[Ad] Endereço:Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455, USA.
[Ti] Título:Triptolide suppresses the in vitro and in vivo growth of lung cancer cells by targeting hyaluronan-CD44/RHAMM signaling.
[So] Source:Oncotarget;8(16):26927-26940, 2017 Apr 18.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Higher levels of hyaluronan (HA) and its receptors CD44 and RHAMM have been associated with poor prognosis and metastasis in NSCLC. In the current study, our goal was to define, using cellular and orthotopic lung tumor models, the role of HA-CD44/RHAMM signaling in lung carcinogenesis and to assess the potential of triptolide to block HA-CD44/RHAMM signaling and thereby suppress the development and progression of lung cancer. Triptolide reduced the viability of five non-small cell lung cancer (NSCLC) cells, the proliferation and self-renewal of pulmospheres, and levels of HA synthase 2 (HAS2), HAS3, HA, CD44, RHAMM, EGFR, Akt and ERK, but increased the cleavage of caspase 3 and PARP. Silencing of HAS2, CD44 or RHAMM induced similar effects. Addition of excess HA to the culture media completely abrogated the effects of triptolide and siRNAs targeting HAS2, CD44, or RHAMM. In an orthotopic lung cancer model in nude rats, intranasal administration of liposomal triptolide (400 µg/kg) for 8 weeks significantly reduced lung tumor growth as determined by bioluminescence imaging, lung weight measurements and gross and histopathological analysis of tumor burden. Also, triptolide suppressed expressions of Ki-67, a marker for cell proliferation, HAS2, HAS3, HA, CD44, and RHAMM in lung tumors. Overall, our results provide a strong rationale for mitigating lung cancer by targeting the HA-CD44/RHAMM signaling axis.
[Mh] Termos MeSH primário: Antineoplásicos Alquilantes/farmacologia
Diterpenos/farmacologia
Proteínas da Matriz Extracelular/metabolismo
Receptores de Hialuronatos/antagonistas & inibidores
Receptores de Hialuronatos/metabolismo
Neoplasias Pulmonares/metabolismo
Fenantrenos/farmacologia
Transdução de Sinais/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Linhagem Celular Tumoral
Movimento Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Modelos Animais de Doenças
Compostos de Epóxi/farmacologia
Inativação Gênica
Seres Humanos
Receptores de Hialuronatos/genética
Hialuronan Sintases/genética
Hialuronan Sintases/metabolismo
Neoplasias Pulmonares/tratamento farmacológico
Neoplasias Pulmonares/genética
Neoplasias Pulmonares/patologia
Masculino
RNA Interferente Pequeno/genética
Ratos
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Alkylating); 0 (CD44 protein, human); 0 (Diterpenes); 0 (Epoxy Compounds); 0 (Extracellular Matrix Proteins); 0 (Hyaluronan Receptors); 0 (Phenanthrenes); 0 (RNA, Small Interfering); 0 (hyaluronan-mediated motility receptor); 19ALD1S53J (triptolide); EC 2.4.1.212 (Hyaluronan Synthases)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.15879


  4 / 12301 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28587572
[Au] Autor:Maffo T; Melong R; Nganteng DND; Wafo P; Ali MS; Ngadjui BT
[Ad] Endereço:a Faculty of Science, Department of Organic Chemistry , University of Yaounde I , Yaoundé , Cameroon.
[Ti] Título:Neomacrodione: a new degraded diterpenoid from the roots of Neoboutonia macrocalyx Beng (Euphorbiaceae).
[So] Source:Nat Prod Res;32(1):85-90, 2018 Jan.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:One new degraded diterpenoid 3,6-dihydroxy-1,7-dimethyl-9,10-phenantroquinone (neomacrodione) (1) together seven known compounds were isolated from the roots of Neoboutonia macrocalyx (Euphorbiaceae). The structures of the compounds were established based on their NMR and mass spectrometric data in conjunction with those previously reported in the literature. Compound (1) displayed moderate antibacterial activities.
[Mh] Termos MeSH primário: Antibacterianos/química
Diterpenos/química
Euphorbiaceae/química
Fenantrenos/química
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Diterpenos/farmacologia
Avaliação Pré-Clínica de Medicamentos/métodos
Espectroscopia de Ressonância Magnética
Testes de Sensibilidade Microbiana
Estrutura Molecular
Fenantrenos/farmacologia
Raízes de Plantas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Diterpenes); 0 (Phenanthrenes)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1335729


  5 / 12301 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28641459
[Au] Autor:Song JL; Yuan Y; Nie LH; Li BL; Qin XB; Li Y; Wu JW; Qiu SX
[Ad] Endereço:a Program for Natural Product Chemical Biology, Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden , Chinese Academy of Sciences , Guangzhou , People's Republic of China.
[Ti] Título:A new ent-kaurane diterpene derivative from the stems of Eurya chinensis R.Br.
[So] Source:Nat Prod Res;32(2):182-188, 2018 Jan.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:One new ent-kaurane diterpene derivative (1), along with four known diterpenes, was isolated from the stems of Eurya chinensis R.Br. The structure of the new compound was established by extensive analysis of mass spectrometric and 1D and 2D NMR spectroscopic data. Compound 1 showed moderate anti-inflammatory activities with IC value of 8.12 µM. This is the first example of diterpenoids with 4-hydroxy-4-(2-hydroxyethyl)-1-hydroxyl-cyclohexanoyl substituent.
[Mh] Termos MeSH primário: Anti-Inflamatórios/isolamento & purificação
Diterpenos Caurânicos/isolamento & purificação
Caules de Planta/química
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Anti-Inflamatórios/química
Anti-Inflamatórios/farmacologia
Diterpenos/química
Diterpenos/isolamento & purificação
Diterpenos Caurânicos/química
Diterpenos Caurânicos/farmacologia
Estrutura Molecular
Análise Espectral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Diterpenes); 0 (Diterpenes, Kaurane)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170624
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2017.1343327


  6 / 12301 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29195901
[Au] Autor:Chen H; Chen Q; Jiang CM; Shi GY; Sui BW; Zhang W; Yang LZ; Li ZY; Liu L; Su YM; Zhao WC; Sun HQ; Li ZZ; Fu Z
[Ad] Endereço:Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, China; China International Science and Technology Cooperation base of Child development and Critical Disorders, China; Chongqing Engineeri
[Ti] Título:Triptolide suppresses paraquat induced idiopathic pulmonary fibrosis by inhibiting TGFB1-dependent epithelial mesenchymal transition.
[So] Source:Toxicol Lett;284:1-9, 2018 Mar 01.
[Is] ISSN:1879-3169
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Idiopathic pulmonary fibrosis (IPF) and tumor are highly similar to abnormal cell proliferation that damages the body. This malignant cell evolution in a stressful environment closely resembles that of epithelial-mesenchymal transition (EMT). As a popular EMT-inducing factor, TGFß plays an important role in the progression of multiple diseases. However, the drugs that target TGFB1 are limited. In this study, we found that triptolide (TPL), a Chinese medicine extract, exerts an anti-lung fibrosis effect by inhibiting the EMT of lung epithelial cells. In addition, triptolide directly binds to TGFß and subsequently increase E-cadherin expression and decrease vimentin expression. In in vivo studies, TPL improves the survival state and inhibits lung fibrosis in mice. In summary, this study revealed the potential therapeutic effect of paraquat induced TPL in lung fibrosis by regulating TGFß-dependent EMT progression.
[Mh] Termos MeSH primário: Diterpenos/uso terapêutico
Medicamentos de Ervas Chinesas/uso terapêutico
Transição Epitelial-Mesenquimal/efeitos dos fármacos
Fibrose Pulmonar Idiopática/prevenção & controle
Paraquat/toxicidade
Fenantrenos/uso terapêutico
Fator de Crescimento Transformador beta1/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Movimento Celular/efeitos dos fármacos
Modelos Animais de Doenças
Diterpenos/farmacologia
Medicamentos de Ervas Chinesas/farmacologia
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/metabolismo
Células Epiteliais/patologia
Compostos de Epóxi/farmacologia
Compostos de Epóxi/uso terapêutico
Seres Humanos
Fibrose Pulmonar Idiopática/induzido quimicamente
Fibrose Pulmonar Idiopática/metabolismo
Fibrose Pulmonar Idiopática/patologia
Pulmão/efeitos dos fármacos
Pulmão/metabolismo
Pulmão/patologia
Camundongos
Simulação de Acoplamento Molecular
Fenantrenos/farmacologia
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Diterpenes); 0 (Drugs, Chinese Herbal); 0 (Epoxy Compounds); 0 (Phenanthrenes); 0 (Tgfb1 protein, mouse); 0 (Transforming Growth Factor beta1); 19ALD1S53J (triptolide); PLG39H7695 (Paraquat)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171203
[St] Status:MEDLINE


  7 / 12301 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29179063
[Au] Autor:Gao X; Mu J; Guan S; Li Q; Du Y; Zhang H; Bi K
[Ad] Endereço:School of Chinese Traditional Medicine, Shenyang Pharmaceutical University, 103Wenhua Road, Shenyang 110016, China.
[Ti] Título:Simultaneous determination of phenolic acids and diterpenoids and their comparative pharmacokinetic study in normal and acute blood stasis rats by UFLC-MS/MS after oral administration of Guan-Xin-Shu-Tong capsules.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1072:221-228, 2018 Jan 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Guan-Xin-Shu-Tong capsules are one of the well-known and first-line Chinese traditional herbal formula for treating coronary heart disease. A validated and sensitive method via ultra fast liquid chromatography-tandem mass spectrometry (UFLC-MS/MS) was established to simultaneously determinate five phenolic acids and four diterpenoids in rats in order to investigate their pharmacokinetic profiles firstly. Analytes were extracted by ethyl acetate and determined via multiple reaction monitoring mode in both positive and negative ion modes. The values for limit of quantification were in range of 0.025-1.250ng/ml. Inter- and intra-day precisions were no more than 10.9% with accuracy of -11.0%-10.6%, meanwhile the stable and suitable extraction recoveries were also obtained. And finally such excellent method was used to compare the pharmacokinetics of nine compounds in normal and acute blood stasis rats after oral administration of Guan-Xin-Shu-Tong capsules.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Diterpenos/sangue
Medicamentos de Ervas Chinesas/administração & dosagem
Hidroxibenzoatos/sangue
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Animais
Diterpenos/química
Diterpenos/farmacocinética
Medicamentos de Ervas Chinesas/farmacocinética
Hidroxibenzoatos/química
Hidroxibenzoatos/farmacocinética
Modelos Lineares
Ratos
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Diterpenes); 0 (Drugs, Chinese Herbal); 0 (Hydroxybenzoates); 29656-58-4 (phenolic acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  8 / 12301 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29262708
[Au] Autor:Kiss T; Mácsai L; Csupor D; Datki ZL
[Ad] Endereço:1 University of Szeged , Faculty of Pharmacy, Department of Pharmacognosy, Eötvös u. 6, H-6720 Szeged , Hungary.
[Ti] Título:In vivo screening of diterpene alkaloids using bdelloid rotifer assays.
[So] Source:Acta Biol Hung;68(4):443-452, 2017 Dec.
[Is] ISSN:0236-5383
[Cp] País de publicação:Hungary
[La] Idioma:eng
[Ab] Resumo:The group of diterpene alkaloids contains numerous compounds with complex chemistry and diverse pharmacological activities. Beside toxicity, these compounds possess activity on the cardiovascular system, tumor cell lines and nervous system. The pharmacological properties have been described using in vitro and in vivo techniques; however, the bioactivities of many compounds have not thoroughly been studied. Here we report on the in vivo evaluation of ten diterpene alkaloids using bdelloid rotifer assays. Napelline exerted toxic effects on rotifers, while wide tolerance range was observed for other investigated compounds. Weak toxicity of songorine is supported by our experiment. Toxicological data for senbusine A, senbusine C, septentrioidine and hetisinone are reported for the first time.
[Mh] Termos MeSH primário: Alcaloides/toxicidade
Diterpenos/toxicidade
Rotíferos/metabolismo
[Mh] Termos MeSH secundário: Animais
Avaliação Pré-Clínica de Medicamentos/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Diterpenes)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE
[do] DOI:10.1556/018.68.2017.4.9


  9 / 12301 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29330051
[Au] Autor:Li X; Lu Q; Xie W; Wang Y; Wang G
[Ad] Endereço:Department of Orthopedics, Wuhan 672 Integrated Traditional Chinese and Western Medicine Hospital, Wuhan, 430079, Hubei, China. Electronic address: lixuguiwuhan@sina.com.
[Ti] Título:Anti-tumor effects of triptolide on angiogenesis and cell apoptosis in osteosarcoma cells by inducing autophagy via repressing Wnt/ß-Catenin signaling.
[So] Source:Biochem Biophys Res Commun;496(2):443-449, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Osteosarcoma is a common malignant bone tumor occurring in adolescents and children. The poor prognosis and low 5-year survival rate of osteosarcoma partly due to high metastasis of osteosarcoma. Triptolide (TPL), an extract from Tripterygium wilfordii, is widely used in cancer treatment. In our present study, we aimed to study the effect of TPL in osteosarcoma treatment and explore the associated regulation mechanism. Our study revealed that TPL inhibited angiogenesis by suppressing the expression of hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in dose dependent manner. Besides, cell apoptosis was induced by TPL obviously in dose dependent manner. Further study demonstrated that TPL induced obvious cell autophagy with increased concentration. The cooperation of autophagy inhibitor 3-MA abolished the effect of TPL on anti-angiogenesis and apoptosis promoting. Moreover, we found that Wnt/ß-Catenin signaling was inactivated by TPL and the adding of pathway inducer Licl neutralized the effect of TPL on autophagy induction, anti-angiogenesis and apoptosis promoting. Taken together, we suggested that TPL inhibited angiogenesis and induced cell apoptosis in osteosarcoma cells by inducing autophagy via repressing Wnt/ß-Catenin signaling.
[Mh] Termos MeSH primário: Antineoplásicos Alquilantes/farmacologia
Autofagia/efeitos dos fármacos
Diterpenos/farmacologia
Regulação Neoplásica da Expressão Gênica
Subunidade alfa do Fator 1 Induzível por Hipóxia/antagonistas & inibidores
Fenantrenos/farmacologia
Fator A de Crescimento do Endotélio Vascular/antagonistas & inibidores
[Mh] Termos MeSH secundário: Adenina/análogos & derivados
Adenina/farmacologia
Apoptose/efeitos dos fármacos
Autofagia/genética
Linhagem Celular Tumoral
Relação Dose-Resposta a Droga
Compostos de Epóxi/farmacologia
Seres Humanos
Subunidade alfa do Fator 1 Induzível por Hipóxia/genética
Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo
Cloreto de Lítio/farmacologia
Osteoblastos/efeitos dos fármacos
Osteoblastos/metabolismo
Osteoblastos/patologia
Transdução de Sinais
Fator A de Crescimento do Endotélio Vascular/genética
Fator A de Crescimento do Endotélio Vascular/metabolismo
beta Catenina/genética
beta Catenina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Alkylating); 0 (CTNNB1 protein, human); 0 (Diterpenes); 0 (Epoxy Compounds); 0 (HIF1A protein, human); 0 (Hypoxia-Inducible Factor 1, alpha Subunit); 0 (Phenanthrenes); 0 (VEGFA protein, human); 0 (Vascular Endothelial Growth Factor A); 0 (beta Catenin); 19ALD1S53J (triptolide); 5142-23-4 (3-methyladenine); G4962QA067 (Lithium Chloride); JAC85A2161 (Adenine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180114
[St] Status:MEDLINE


  10 / 12301 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29288766
[Au] Autor:Wang J; Zhang Z; Li R; Sun W; Chen J; Zhang H; Shu K; Lei T
[Ad] Endereço:Sino-German Neuro-Oncology Molecular Laboratory, Department of Neurosurgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
[Ti] Título:Triptolide inhibits pituitary adenoma cell viability, migration and invasion via ADAM12/EGFR signaling pathway.
[So] Source:Life Sci;194:150-156, 2018 Feb 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIM: Triptolide, an effective component derived from Tripterygium wilfordii, has been well recognized to process a broad-spectrum antitumor activities in various tumor types. However, the potential role of triptolide in pituitary adenomas remains unknown. The aim of this study was to investigate the precise role of triptolide and underlying mechanism in regulating pituitary adenoma cell viability, migration and invasion. MAIN METHODS: We use mouse pituitary adenoma cells (TtT/GF and AtT20 cells) as the experiment model and treated them with varying concentrations of triptolide. The corresponding inhibitory effects on cell viability, migration, invasion and apoptosis were examined respectively, and the underlying mechanism was determined by investigating ADAM12 (a disintegrin and metalloprotease 12)/EGFR signaling. KEY FINDINGS: Triptolide significantly inhibited cell viability, migration and invasion in TtT/GF and AtT20 cells in a dose-dependent manner. Mechanistically, triptolide significantly reduced ADAM12 expression at protein levels and attenuated ADAM12/EGFR signaling. Meanwhile, triptolide treatment combined with ADAM12 silencing enhanced the suppression effects on cell viability, migration and invasion, and those effects were restored following ADAM12-rescued. Moreover, triptolide suppressed the tumorigenesis of TtT/GF and AtT20 cells in vivo. SIGNIFICANCE: Our research provides evidence that triptolide inhibits pituitary adenoma cell viability, migration and invasion via ADAM12/EGFR signaling pathway. These findings suggest a potential role for triptolide in treating pituitary adenomas.
[Mh] Termos MeSH primário: Proteína ADAM12/metabolismo
Adenoma/dietoterapia
Antineoplásicos Alquilantes/farmacologia
Sobrevivência Celular/efeitos dos fármacos
Diterpenos/farmacologia
Fenantrenos/farmacologia
Neoplasias Hipofisárias/tratamento farmacológico
Receptor do Fator de Crescimento Epidérmico/metabolismo
[Mh] Termos MeSH secundário: Adenoma/metabolismo
Adenoma/patologia
Animais
Linhagem Celular Tumoral
Movimento Celular/efeitos dos fármacos
Compostos de Epóxi/farmacologia
Camundongos
Hipófise/efeitos dos fármacos
Hipófise/metabolismo
Hipófise/patologia
Neoplasias Hipofisárias/metabolismo
Neoplasias Hipofisárias/patologia
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Alkylating); 0 (Diterpenes); 0 (Epoxy Compounds); 0 (Phenanthrenes); 19ALD1S53J (triptolide); EC 2.7.10.1 (Receptor, Epidermal Growth Factor); EC 3.4.24.- (ADAM12 Protein)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171231
[St] Status:MEDLINE



página 1 de 1231 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde