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Pesquisa : D02.455.849.291.239.500 [Categoria DeCS]
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[PMID]:29261746
[Au] Autor:Pan R; Xu L; Wei Q; Wu C; Tang W; Oelmüller R; Zhang W
[Ad] Endereço:Hubei Collaborative Innovation Center for Grain Industry/ Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, China.
[Ti] Título:Piriformospora indica promotes early flowering in Arabidopsis through regulation of the photoperiod and gibberellin pathways.
[So] Source:PLoS One;12(12):e0189791, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Flowering in plants is synchronized by both environmental cues and internal regulatory factors. Previous studies have shown that the endophytic fungus Piriformospora indica promotes the growth and early flowering in Coleus forskohlii (a medicinal plant) and Arabidopsis. To further dissect the impact of P. indica on pathways responsible for flowering time in Arabidopsis, we co-cultivated Arabidopsis with P. indica and used RT-qPCR to analyze the main gene regulation networks involved in flowering. Our results revealed that the symbiotic interaction of Arabidopsis with P. indica promotes early flower development and the number of siliques. In addition, expression of the core flowering regulatory gene FLOWERING LOCUS T (FT), of genes controlling the photoperiod [CRYPTOCHROMES (CRY1, CRY2) and PHYTOCHROME B (PHYB)] and those related to gibberellin (GA) functions (RGA1, AGL24, GA3, and MYB5) were induced by the fungus, while key genes controlling the age and autonomous pathways remained unchanged. Moreover, early flowering promotion conferred by P. indica was promoted by exogenous GA and inhabited by GA inhibitor, and this effect could be observed under long day and neutral day photoperiod. Therefore, our data suggested that P. indica promotes early flowering in Arabidopsis likely through photoperiod and GA rather than age or the autonomous pathway.
[Mh] Termos MeSH primário: Arabidopsis/microbiologia
Arabidopsis/fisiologia
Basidiomycota/fisiologia
Flores/fisiologia
Giberelinas/metabolismo
Fotoperíodo
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Flores/efeitos dos fármacos
Flores/genética
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Giberelinas/farmacologia
Fenótipo
Desenvolvimento Vegetal/efeitos dos fármacos
Desenvolvimento Vegetal/genética
Solo
Fatores de Tempo
Triazóis/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gibberellins); 0 (Soil); 0 (Triazoles); R4ATA06H50 (uniconazole)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189791


  2 / 2860 MEDLINE  
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[PMID]:28448840
[Au] Autor:Bhattacharyya D; Lee YH
[Ad] Endereço:Division of Biotechnology, Chonbuk National University, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596, Republic of Korea.
[Ti] Título:A cocktail of volatile compounds emitted from Alcaligenes faecalis JBCS1294 induces salt tolerance in Arabidopsis thaliana by modulating hormonal pathways and ion transporters.
[So] Source:J Plant Physiol;214:64-73, 2017 Jul.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In our previous study we showed that volatile organic compounds (VOCs) from Alcaligenes faecalis JBCS1294 (JBCS1294) induced tolerance to salt stress in Arabidopsis thaliana by influencing the auxin and gibberellin pathways and upregulating the expression of key ion transporters. The aim of this study was to evaluate the contribution of each VOC and blends of the VOCs on the induction of salt tolerance and signaling pathways. The key VOCs emitted from JBCS1294 were dissolved in lanolin and applied to one side of bipartite I-plates that contained Arabidopsis seeds on Murashige and Skoog (MS) media supplemented with NaCl on the other side. Changes in plant growth were investigated using Arabidopsis mutant lines and hormone inhibitors, and gene expression was assessed by real-time PCR (qPCR). Among the VOCs, butyric acid conferred salt tolerance over a concentration range of 5.6µM (10ng)-56mM (100µg), whereas propionic and benzoic acid were effective at micromolar doses. Intriguingly, the optimized cocktail of the three VOCs increased fresh weight of Arabidopsis under salt stress compared to that achieved with each single compound. However, Arabidopsis growth was not promoted by the VOCs without salt stress. Exogenous indole-3-acetic acid (IAA) application arrested salt tolerance or growth promotion of Arabidopsis induced by volatiles from propionic acid, but not from butyric acid and an optimized volatile mixture of butyric acid, propionic acid, and benzoic acid (1PBB). High and intense auxin-responsive DR5:GUS activity was observed in the roots of Arabidopsis grown on media without salt via 1PBB, butyric acid, and benzoic acid. Growth promotion by the cocktail was inhibited in the eir1 mutant and in Col-0 plants treated with inhibitors of auxin and gibberellin. The present study clearly demonstrated the effects of individual VOCs and blends of VOCs from a rhizobacterial strain on the induction of salt stress. The results with the blend of VOCs, which mimics bacterial emissions in nature, may lead to a deeper understanding of the interaction between rhizobacteria and plants.
[Mh] Termos MeSH primário: Alcaligenes faecalis/química
Arabidopsis/efeitos dos fármacos
Arabidopsis/metabolismo
Compostos Orgânicos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Giberelinas/metabolismo
Ácidos Indolacéticos/metabolismo
Tolerância a Sal/efeitos dos fármacos
Compostos Orgânicos Voláteis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gibberellins); 0 (Indoleacetic Acids); 0 (Volatile Organic Compounds)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE


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[PMID]:29053751
[Au] Autor:Ouyang E; Lu Y; Ouyang J; Wang L; Wang X
[Ad] Endereço:School of Civil Engineering and Architecture, Nanchang University, Nanchang, China.
[Ti] Título:Bacterial community analysis of anoxic/aeration (A/O) system in a combined process for gibberellin wastewater treatment.
[So] Source:PLoS One;12(10):e0186743, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gibberellin wastewater cannot be directly discharged without treatment due to its high concentrations of sulfate and organic compounds and strong acidity. Therefore, multi-stage anaerobic bioreactor + micro-aerobic+ anoxic/aeration (A/O) + biological contact oxidation combined processes are used to treat gibberellin wastewater. However, knowledge of the treatment effects of the A/O process and bacterial community structure in the aeration tank reactors of such systems is sparse. Therefore, this study was conducted to investigate the treatment effects and operation of the A/O process on gibberellin wastewater, as well as changes in the bacterial community structure of activated sludge in the aeration tank during treatment. Moreover, removal was examined based on evaluation of effluent after A/O treatment. Although influent chemical oxygen demand (COD), NH3-N and total phosphorus (TP) fluctuated, effluent COD, NH3-N and TP remained stable. Moreover, average COD, NH3-N and TP removal efficiency were 68.41%, 93.67% and 45.82%, respectively, during the A/O process. At the phylum level, Proteobacteria was the dominant phylum in all samples, followed by Chloroflexi, Bacteroidetes and Actinobacteria. Proteobacteria played an important role in the removal of organic matter. Chloroflexi was found to be responsible for the degradation of carbohydrates and Bacteroidetes also had been found to be responsible for the degradation of complex organic matters. Actinobacteria are able to degrade a variety of environmental chemicals. Additionally, Anaerolineaceae_uncultured was the major genus in samples collected on May 25, 2015, while Novosphingobium and Nitrospira were dominant in most samples. Nitrosomonas are regarded as the dominant ammonia-oxidizing bacteria, while Nitrospira are the main nitrite-oxidizing bacteria. Bacterial community structure varied considerably with time, and a partial Mantel test showed a highly significant positive correlation between bacterial community structure and DO. The bacterial community structure was also positively correlated with temperature and SO42-.
[Mh] Termos MeSH primário: Bactérias/metabolismo
Giberelinas/metabolismo
Oxigênio/metabolismo
Águas Residuais/microbiologia
Microbiologia da Água
[Mh] Termos MeSH secundário: Reatores Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gibberellins); 0 (Waste Water); S88TT14065 (Oxygen)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171021
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186743


  4 / 2860 MEDLINE  
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[PMID]:28953930
[Au] Autor:Bai Z; Xia P; Wang R; Jiao J; Ru M; Liu J; Liang Z
[Ad] Endereço:College of Life Science, Northwest A&F University, Yangling, China.
[Ti] Título:Molecular cloning and characterization of five SmGRAS genes associated with tanshinone biosynthesis in Salvia miltiorrhiza hairy roots.
[So] Source:PLoS One;12(9):e0185322, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The gibberellin-responsive element binding factor (GRAS) family of proteins plays an important role in the transcriptional regulation of plant development and hormone signaling. To date, there are no reports on GRAS family proteins expressed in Salvia miltiorrhiza. In this study, 28 ESTs that contained the GRAS domain were identified from a S. miltiorrhiza cDNA library. Of these, full-length sequences of five genes were cloned and sequence analysis indicated that all five proteins contain only one GRAS domain and therefore, belong to the GRAS family. The five genes were designated S. miltiorrhiza GRAS1-5 (SmGRAS1-5), which belong to groups I (SmGRAS2 and SmGRAS4), II (SmGRAS3), III (SmGRAS1), and VIII (SmGRAS5) respectively. Additionally, SmGRAS1-5 have different expression patterns in the reed head, stems, leaves, flowers, and roots of S. miltiorrhiza. In this study, the expression of SmGRAS1-5 was sensitive to Gibberellin (GA) stress and that of SmGRAS1, SmGRAS4 and SmGRAS5 was sensitive to Ethephon (Eth) stress respectively. Moreover, S. miltiorrhiza copalyl diphosphate synthases 1 (SmCPS1) and S. miltiorrhiza kaurene synthase like 1 (SmKSL1), which are two key enzymes gene in the diterpenoid biosynthesis pathway, were also response to GA and Eth stress. In addition, Dihydrotanshinone (DT-I) and Tanshinone I (T-I) content were enhanced by GA and Eth stress, Tanshinone IIA (T-IIA) content was increased by GA stress, and the accumulation of Cryptotanshinone (CT) was insensitive to both GA and Eth stress. Together, these results provide insights into functional conservation and diversification of SmGRASs and are useful information for further elucidating SmGRAS functions.
[Mh] Termos MeSH primário: Vias Biossintéticas/genética
Diterpenos Abietanos/biossíntese
Genes de Plantas
Proteínas de Plantas/genética
Raízes de Plantas/genética
Salvia miltiorrhiza/genética
[Mh] Termos MeSH secundário: Motivos de Aminoácidos
Sequência de Aminoácidos
Sequência de Bases
Vias Biossintéticas/efeitos dos fármacos
Clonagem Molecular
Sequência Conservada/genética
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Giberelinas/farmacologia
Especificidade de Órgãos/efeitos dos fármacos
Especificidade de Órgãos/genética
Compostos Organofosforados/farmacologia
Fenantrenos/metabolismo
Filogenia
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
Raízes de Plantas/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Diterpenes, Abietane); 0 (Gibberellins); 0 (Organophosphorus Compounds); 0 (Phenanthrenes); 0 (Plant Proteins); 03UUH3J385 (tanshinone); XU5R5VQ87S (ethephon)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185322


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[PMID]:28750086
[Au] Autor:Igielski R; Kepczynska E
[Ad] Endereço:Department of Plant Biotechnology, University of Szczecin, Szczecin, Poland.
[Ti] Título:Gene expression and metabolite profiling of gibberellin biosynthesis during induction of somatic embryogenesis in Medicago truncatula Gaertn.
[So] Source:PLoS One;12(7):e0182055, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gibberellins (GAs) are involved in the regulation of numerous developmental processes in plants including zygotic embryogenesis, but their biosynthesis and role during somatic embryogenesis (SE) is mostly unknown. In this study we show that during three week- long induction phase, when cells of leaf explants from non-embryogenic genotype (M9) and embryogenic variant (M9-10a) were forming the callus, all the bioactive gibberellins from non-13-hydroxylation (GA4, GA7) and 13-hydroxylation (GA1, GA5, GA3, GA6) pathways were present, but the contents of only a few of them differed between the tested lines. The GA53 and GA19 substrates synthesized by the 13-hydroxylation pathway accumulated specifically in the M9-10a line after the first week of induction; subsequently, among the bioactive gibberellins detected, only the content of GA3 increased and appeared to be connected with acquisition of embryogenic competence. We fully annotated 20 Medicago truncatula orthologous genes coding the enzymes which catalyze all the known reactions of gibberellin biosynthesis. Our results indicate that, within all the genes tested, expression of only three: MtCPS, MtGA3ox1 and MtGA3ox2, was specific to embryogenic explants and reflected the changes observed in GA53, GA19 and GA3 contents. Moreover, by analyzing expression of MtBBM, SE marker gene, we confirmed the inhibitory effect of manipulation in GAs metabolism, applying exogenous GA3, which not only impaired the production of somatic embryos, but also significantly decreased expression of this gene.
[Mh] Termos MeSH primário: Perfilação da Expressão Gênica/métodos
Giberelinas/biossíntese
Medicago truncatula/genética
Medicago truncatula/metabolismo
Metabolômica/métodos
Técnicas de Embriogênese Somática de Plantas
[Mh] Termos MeSH secundário: Vias Biossintéticas/efeitos dos fármacos
Vias Biossintéticas/genética
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Genes de Plantas
Medicago truncatula/efeitos dos fármacos
Medicago truncatula/embriologia
Folhas de Planta/anatomia & histologia
Folhas de Planta/genética
Folhas de Planta/metabolismo
Sementes/efeitos dos fármacos
Sementes/genética
Sementes/metabolismo
Triazóis/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gibberellins); 0 (Triazoles); 6PLV42R3ZA (paclobutrazol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182055


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[PMID]:28716493
[Au] Autor:Jiang K; Shimotakahara H; Luo M; Otani M; Nakamura H; Moselhy SS; Abualnaja KO; Al-Malki AL; Kumosani TA; Kitahata N; Nakano T; Nakajima M; Asami T
[Ad] Endereço:Department of Applied Biological Chemistry, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
[Ti] Título:Chemical screening and development of novel gibberellin mimics.
[So] Source:Bioorg Med Chem Lett;27(16):3678-3682, 2017 08 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Gibberellin (GA) plays versatile roles in the regulation of plant growth and development and therefore is widely used as a regulator in agriculture. We performed a chemical library screening and identified a chemical, named 67D, as a stimulator of seed germination that was suppressed by paclobutrazol (PAC), a GA biosynthesis inhibitor. In vitro binding assays indicated that 67D binds to the GID1 receptor. Further studies on the structure-activity relationship identified a chemical, named chemical 6, that strongly promoted seed germination suppressed by PAC. Chemical 6 was further confirmed to promote the degradation of RGA (for repressor of ga1-3), a DELLA protein, and suppress the expression levels of GA3ox1 in the same manner as GA does. 67D and its analogs are supposed to be agonists of GID1 and are expected to be utilized in agriculture and basic research as an alternative to GA.
[Mh] Termos MeSH primário: Giberelinas/química
Bibliotecas de Moléculas Pequenas/química
[Mh] Termos MeSH secundário: Arabidopsis/efeitos dos fármacos
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/agonistas
Proteínas de Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Germinação/efeitos dos fármacos
Germinação/efeitos da radiação
Giberelinas/síntese química
Giberelinas/farmacologia
Luz
Reguladores de Crescimento de Planta/química
Reguladores de Crescimento de Planta/farmacologia
Receptores de Superfície Celular/agonistas
Receptores de Superfície Celular/genética
Receptores de Superfície Celular/metabolismo
Sementes/efeitos dos fármacos
Sementes/crescimento & desenvolvimento
Bibliotecas de Moléculas Pequenas/síntese química
Bibliotecas de Moléculas Pequenas/farmacologia
Relação Estrutura-Atividade
Triazóis/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (GAI protein, Arabidopsis); 0 (GID1b protein, Arabidopsis); 0 (Gibberellins); 0 (Plant Growth Regulators); 0 (Receptors, Cell Surface); 0 (Small Molecule Libraries); 0 (Triazoles); 6PLV42R3ZA (paclobutrazol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE


  7 / 2860 MEDLINE  
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[PMID]:28692266
[Au] Autor:Wang L; Zhao R; Zheng Y; Chen L; Li R; Ma J; Hong X; Ma P; Sheng J; Shen L
[Ad] Endereço:College of Food Science and Nutritional Engineering, China Agricultural University , Beijing 100083, China.
[Ti] Título:SlMAPK1/2/3 and Antioxidant Enzymes Are Associated with H O -Induced Chilling Tolerance in Tomato Plants.
[So] Source:J Agric Food Chem;65(32):6812-6820, 2017 Aug 16.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hydrogen peroxide (H O ) acts as a signaling molecule in response to cold stress. Mitogen-activated protein kinases (MAPKs) and C-repeat/dehydration-responsive factor (CBF) play important roles in cold response regulation. To investigate the roles of MAPKs and CBF in H O -induced chilling tolerance, tomato (Solanum lycopersicum cv. Ailsa Craig) plants were treated with 1 mM H O before chilling treatment. The results showed that H O treatment protected subcellular structure, increased concentrations of abscisic acid (ABA), zeatin riboside (ZR), and methyl jasmonate (MeJA), but decreased the concentration of gibberellic acid (GA ). Furthermore, 1 mM H O treatment enhanced the activities of antioxidant enzymes. Meanwhile, relative expressions of SlMAPK1/2/3 and SlCBF1 in H O -treated plants were higher than those in the control. Our findings suggest that H O treatment might enhance the chilling tolerance of tomato plants by activating SlMAPK1/2/3 and SlCBF1 gene expression and by regulating phytohormone concentrations and antioxidant enzyme activities.
[Mh] Termos MeSH primário: Antioxidantes/metabolismo
Peróxido de Hidrogênio/farmacologia
Lycopersicon esculentum/enzimologia
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Ácido Abscísico/metabolismo
Temperatura Baixa
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Giberelinas/metabolismo
Lycopersicon esculentum/efeitos dos fármacos
Lycopersicon esculentum/genética
Proteínas Quinases Ativadas por Mitógeno/genética
Reguladores de Crescimento de Planta/metabolismo
Proteínas de Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Gibberellins); 0 (Plant Growth Regulators); 0 (Plant Proteins); 72S9A8J5GW (Abscisic Acid); BBX060AN9V (Hydrogen Peroxide); BU0A7MWB6L (gibberellic acid); EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170825
[Lr] Data última revisão:
170825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01685


  8 / 2860 MEDLINE  
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[PMID]:28668394
[Au] Autor:Kim D; Abdelaziz ME; Ntui VO; Guo X; Al-Babili S
[Ad] Endereço:King Abdullah University of Science and Technology (KAUST), BESE Division, Thuwal, 23955-6900, Saudi Arabia.
[Ti] Título:Colonization by the endophyte Piriformospora indica leads to early flowering in Arabidopsis thaliana likely by triggering gibberellin biosynthesis.
[So] Source:Biochem Biophys Res Commun;490(4):1162-1167, 2017 Sep 02.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Piriformospora indica is an endophytic fungus colonizing roots of a wide variety of plants. Previous studies showed that P. indica promotes early flowering and plant growth in the medicinal plant Coleus forskohlii. To determine the impact of P. indica on flowering time in Arabidopsis, we co-cultivated the plants with P. indica under long day condition. P. indica inoculated Arabidopsis plants displayed significant early flowering phenotype. qRT-PCR analysis of colonized plants revealed an up-regulation of flowering regulatory (FLOWERING LOCUS T, LEAFY, and APETALA1) and gibberellin biosynthetic (Gibberellin 20-Oxidase2, Gibberellin 3-Oxidase1 and Gibberellin requiring1) genes, while the flowering-repressing gene FLOWERING LOCUS C was down regulated. Quantification of gibberellins content showed that the colonization with P. indica caused an increase in GA content. Compared to wild-type plants, inoculation of the Arabidopsis ga5 mutant affected in gibberellin biosynthetic gene led to less pronounced changes in the expression of genes regulating flowering and to a lower increase in GA content. Taken together, our data indicate that P. indica promotes early flowering in Arabidopsis likely by increasing gibberellin content.
[Mh] Termos MeSH primário: Arabidopsis/metabolismo
Basidiomycota/metabolismo
Endófitos/metabolismo
Flores/metabolismo
Giberelinas/biossíntese
Raízes de Plantas/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/microbiologia
Flores/microbiologia
Raízes de Plantas/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gibberellins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170703
[St] Status:MEDLINE


  9 / 2860 MEDLINE  
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[PMID]:28628628
[Au] Autor:Nelson SK; Steber CM
[Ad] Endereço:Molecular Plant Sciences Program, Washington State University, Pullman, Washington, United States of America.
[Ti] Título:Transcriptional mechanisms associated with seed dormancy and dormancy loss in the gibberellin-insensitive sly1-2 mutant of Arabidopsis thaliana.
[So] Source:PLoS One;12(6):e0179143, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:While widespread transcriptome changes were previously observed with seed dormancy loss, this study specifically characterized transcriptional changes associated with the increased seed dormancy and dormancy loss of the gibberellin (GA) hormone-insensitive sleepy1-2 (sly1-2) mutant. The SLY1 gene encodes the F-box subunit of an SCF E3 ubiquitin ligase needed for GA-triggered proteolysis of DELLA repressors of seed germination. DELLA overaccumulation in sly1-2 seeds leads to increased dormancy that can be rescued without DELLA protein destruction either by overexpression of the GA receptor, GA-INSENSITIVE DWARF1b (GID1b-OE) (74% germination) or by extended dry after-ripening (11 months, 51% germination). After-ripening of sly1 resulted in different transcriptional changes in early versus late Phase II of germination that were consistent with the processes known to occur. Approximately half of the transcriptome changes with after-ripening appear to depend on SLY1-triggered DELLA proteolysis. Given that many of these SLY1/GA-dependent changes are genes involved in protein translation, it appears that GA signaling increases germination capacity in part by activating translation. While sly1-2 after-ripening was associated with transcript-level changes in 4594 genes over two imbibition timepoints, rescue of sly1-2 germination by GID1b-OE was associated with changes in only 23 genes. Thus, a big change in sly1-2 germination phenotype can occur with relatively little change in the global pattern of gene expression during the process of germination. Most GID1b-OE-responsive transcripts showed similar changes with after-ripening in early Phase II of imbibition, but opposite changes with after-ripening by late Phase II. This suggests that GID1b-OE stimulates germination early in imbibition, but may later trigger negative feedback regulation.
[Mh] Termos MeSH primário: Alquil e Aril Transferases/metabolismo
Proteínas de Arabidopsis/metabolismo
Arabidopsis/efeitos dos fármacos
Giberelinas/farmacologia
Dormência de Plantas/efeitos dos fármacos
Transcriptoma/efeitos dos fármacos
[Mh] Termos MeSH secundário: Alquil e Aril Transferases/genética
Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/genética
Germinação/efeitos dos fármacos
Mutação
Fenótipo
Reguladores de Crescimento de Planta/farmacologia
Plantas Geneticamente Modificadas/metabolismo
Proteólise
RNA de Plantas/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Receptores de Superfície Celular/genética
Receptores de Superfície Celular/metabolismo
Sementes/efeitos dos fármacos
Sementes/genética
Sementes/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (GAI protein, Arabidopsis); 0 (GID1b protein, Arabidopsis); 0 (Gibberellins); 0 (Plant Growth Regulators); 0 (RNA, Plant); 0 (Receptors, Cell Surface); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.29 (SLY1 protein, Arabidopsis)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179143


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[PMID]:28494021
[Au] Autor:Tao T; Zhou CJ; Wang Q; Chen XR; Sun Q; Zhao TY; Ye JC; Wang Y; Zhang ZY; Zhang YL; Guo ZJ; Wang XB; Li DW; Yu JL; Han CG
[Ad] Endereço:State Key Laboratory for Agro-biotechnology and Ministry of Agriculture Key Laboratory for Plant Pathology, China Agricultural University, Beijing, People's Republic of China.
[Ti] Título:Rice black streaked dwarf virus P7-2 forms a SCF complex through binding to Oryza sativa SKP1-like proteins, and interacts with GID2 involved in the gibberellin pathway.
[So] Source:PLoS One;12(5):e0177518, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:As a core subunit of the SCF complex that promotes protein degradation through the 26S proteasome, S-phase kinase-associated protein 1 (SKP1) plays important roles in multiple cellular processes in eukaryotes, including gibberellin (GA), jasmonate, ethylene, auxin and light responses. P7-2 encoded by Rice black streaked dwarf virus (RBSDV), a devastating viral pathogen that causes severe symptoms in infected plants, interacts with SKP1 from different plants. However, whether RBSDV P7-2 forms a SCF complex and targets host proteins is poorly understood. In this study, we conducted yeast two-hybrid assays to further explore the interactions between P7-2 and 25 type I Oryza sativa SKP1-like (OSK) proteins, and found that P7-2 interacted with eight OSK members with different binding affinity. Co-immunoprecipitation assay further confirmed the interaction of P7-2 with OSK1, OSK5 and OSK20. It was also shown that P7-2, together with OSK1 and O. sativa Cullin-1, was able to form the SCF complex. Moreover, yeast two-hybrid assays revealed that P7-2 interacted with gibberellin insensitive dwarf2 (GID2) from rice and maize plants, which is essential for regulating the GA signaling pathway. It was further demonstrated that the N-terminal region of P7-2 was necessary for the interaction with GID2. Overall, these results indicated that P7-2 functioned as a component of the SCF complex in rice, and interaction of P7-2 with GID2 implied possible roles of the GA signaling pathway during RBSDV infection.
[Mh] Termos MeSH primário: Giberelinas/metabolismo
Complexos Multiproteicos/metabolismo
Oryza/metabolismo
Oryza/virologia
Proteínas de Plantas/metabolismo
Reoviridae/metabolismo
Proteínas Quinases Associadas a Fase S/metabolismo
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Imunoprecipitação
Folhas de Planta/metabolismo
Ligação Proteica
Reprodutibilidade dos Testes
Tabaco/metabolismo
Técnicas do Sistema de Duplo-Híbrido
Zea mays
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gibberellins); 0 (Multiprotein Complexes); 0 (Plant Proteins); 0 (S-Phase Kinase-Associated Proteins); 0 (Viral Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177518



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