Base de dados : MEDLINE
Pesquisa : D02.455.849.842 [Categoria DeCS]
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  1 / 291 MEDLINE  
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[PMID]:28621941
[Au] Autor:Afifi AH; Kagiyama I; El-Desoky AH; Kato H; Mangindaan REP; de Voogd NJ; Ammar NM; Hifnawy MS; Tsukamoto S
[Ad] Endereço:Graduate School of Pharmaceutical Sciences, Kumamoto University , 5-1 Oe-honmachi, Kumamoto 862-0973, Japan.
[Ti] Título:Sulawesins A-C, Furanosesterterpene Tetronic Acids That Inhibit USP7, from a Psammocinia sp. Marine Sponge.
[So] Source:J Nat Prod;80(7):2045-2050, 2017 Jul 28.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Three new furanosesterterpene tetronic acids, sulawesins A-C (1-3), were isolated from a Psammocinia sp. marine sponge, along with the known compounds ircinins-1 (4) and -2 (5). Although ircinins-1 and -2 were previously isolated as (+)- or (-)-enantiomers from marine sponges, we isolated them as enantiomeric mixtures. Sulawesins A and B possess a new carbon skeleton with a 5-(furan-3-yl)-4-hydroxycyclopent-2-enone moiety and were also found to be diastereomeric mixtures of four isomers by an HPLC analysis with a chiral-phase column. Sulawesin C has a dimeric structure of ircinin-1 and is the first dimer in this family. USP7, a deubiquitinating enzyme, is an emergent target of cancer therapy, and the isolated compounds inhibited USP7 with IC values in the range of 2.7-4.6 µM.
[Mh] Termos MeSH primário: Furanos/isolamento & purificação
Furanos/farmacologia
Poríferos/química
Sesterterpenos/isolamento & purificação
Sesterterpenos/farmacologia
Terpenos/isolamento & purificação
Terpenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Furanos/química
Seres Humanos
Indonésia
Concentração Inibidora 50
Biologia Marinha
Estrutura Molecular
Sesquiterpenos
Sesterterpenos/química
Estereoisomerismo
Relação Estrutura-Atividade
Terpenos/química
Ubiquitina Tiolesterase
Peptidase 7 Específica de Ubiquitina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Furans); 0 (Sesquiterpenes); 0 (Sesterterpenes); 0 (Terpenes); 0 (ircinin-1); 0 (sulawesin A); 0 (sulawesin B); 0 (sulawesin C); EC 3.4.19.12 (USP7 protein, human); EC 3.4.19.12 (Ubiquitin Thiolesterase); EC 3.4.19.12 (Ubiquitin-Specific Peptidase 7); N2B9NB89C0 (tetronic acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.7b00184


  2 / 291 MEDLINE  
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[PMID]:28604695
[Au] Autor:Clevenger KD; Bok JW; Ye R; Miley GP; Verdan MH; Velk T; Chen C; Yang K; Robey MT; Gao P; Lamprecht M; Thomas PM; Islam MN; Palmer JM; Wu CC; Keller NP; Kelleher NL
[Ad] Endereço:Department of Chemistry, Northwestern University, Evanston, Illinois, USA.
[Ti] Título:A scalable platform to identify fungal secondary metabolites and their gene clusters.
[So] Source:Nat Chem Biol;13(8):895-901, 2017 Aug.
[Is] ISSN:1552-4469
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The genomes of filamentous fungi contain up to 90 biosynthetic gene clusters (BGCs) encoding diverse secondary metabolites-an enormous reservoir of untapped chemical potential. However, the recalcitrant genetics, cryptic expression, and unculturability of these fungi prevent scientists from systematically exploiting these gene clusters and harvesting their products. As heterologous expression of fungal BGCs is largely limited to the expression of single or partial clusters, we established a scalable process for the expression of large numbers of full-length gene clusters, called FAC-MS. Using fungal artificial chromosomes (FACs) and metabolomic scoring (MS), we screened 56 secondary metabolite BGCs from diverse fungal species for expression in Aspergillus nidulans. We discovered 15 new metabolites and assigned them with confidence to their BGCs. Using the FAC-MS platform, we extensively characterized a new macrolactone, valactamide A, and its hybrid nonribosomal peptide synthetase-polyketide synthase (NRPS-PKS). The ability to regularize access to fungal secondary metabolites at an unprecedented scale stands to revitalize drug discovery platforms with renewable sources of natural products.
[Mh] Termos MeSH primário: Aspergillus/genética
Aspergillus/metabolismo
Genes Fúngicos/genética
Família Multigênica
Metabolismo Secundário/genética
Sesterterpenos/análise
[Mh] Termos MeSH secundário: Benzodiazepinas/análise
Benzodiazepinas/metabolismo
Pirimidinonas/análise
Pirimidinonas/metabolismo
Sesterterpenos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pyrimidinones); 0 (Sesterterpenes); 12794-10-4 (Benzodiazepines); 157047-96-6 (benzomalvin A); 157047-97-7 (benzomalvin B)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE
[do] DOI:10.1038/nchembio.2408


  3 / 291 MEDLINE  
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[PMID]:28362483
[Au] Autor:Pemberton TA; Chen M; Harris GG; Chou WK; Duan L; Köksal M; Genshaft AS; Cane DE; Christianson DW
[Ad] Endereço:Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania , 231 South 34th Street, Philadelphia, Pennsylvania 19104-6323, United States.
[Ti] Título:Exploring the Influence of Domain Architecture on the Catalytic Function of Diterpene Synthases.
[So] Source:Biochemistry;56(14):2010-2023, 2017 Apr 11.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Terpenoid synthases catalyze isoprenoid cyclization reactions underlying the generation of more than 80,000 natural products. Such dramatic chemodiversity belies the fact that these enzymes generally consist of only three domain folds designated as α, ß, and γ. Catalysis by class I terpenoid synthases occurs exclusively in the α domain, which is found with α, αα, αß, and αßγ domain architectures. Here, we explore the influence of domain architecture on catalysis by taxadiene synthase from Taxus brevifolia (TbTS, αßγ), fusicoccadiene synthase from Phomopsis amygdali (PaFS, (αα) ), and ophiobolin F synthase from Aspergillus clavatus (AcOS, αα). We show that the cyclization fidelity and catalytic efficiency of the α domain of TbTS are severely compromised by deletion of the ßγ domains; however, retention of the ß domain preserves significant cyclization fidelity. In PaFS, we previously demonstrated that one α domain similarly influences catalysis by the other α domain [ Chen , M. , Chou , W. K. W. , Toyomasu , T. , Cane , D. E. , and Christianson , D. W. ( 2016 ) ACS Chem. Biol. 11 , 889 - 899 ]. Here, we show that the hexameric quaternary structure of PaFS enables cluster channeling. We also show that the α domains of PaFS and AcOS can be swapped so as to make functional chimeric αα synthases. Notably, both cyclization fidelity and catalytic efficiency are altered in all chimeric synthases. Twelve newly formed and uncharacterized C diterpene products and three C sesterterpene products are generated by these chimeras. Thus, engineered αßγ and αα terpenoid cyclases promise to generate chemodiversity in the greater family of terpenoid natural products.
[Mh] Termos MeSH primário: Alquil e Aril Transferases/química
Aspergillus/genética
Isomerases/química
Proteínas Mutantes Quiméricas/química
Saccharomycetales/genética
Taxus/genética
[Mh] Termos MeSH secundário: Alquil e Aril Transferases/genética
Alquil e Aril Transferases/metabolismo
Aspergillus/enzimologia
Ciclização
Diterpenos/metabolismo
Expressão Gênica
Isomerases/genética
Isomerases/metabolismo
Cinética
Modelos Moleculares
Proteínas Mutantes Quiméricas/genética
Proteínas Mutantes Quiméricas/metabolismo
Domínios Proteicos
Engenharia de Proteínas
Estrutura Secundária de Proteína
Saccharomycetales/enzimologia
Sesterterpenos/biossíntese
Taxus/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Diterpenes); 0 (Mutant Chimeric Proteins); 0 (Sesterterpenes); 0 (fusicoccadiene); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.- (ophiobolin F synthase, Aspergillus clavatus); EC 5.- (Isomerases); EC 5.- (taxa-4(5),11(12)-diene synthase)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00137


  4 / 291 MEDLINE  
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[PMID]:28192519
[Au] Autor:Paillamanque J; Sanchez-Tusie A; Carmona EM; Treviño CL; Sandoval C; Nualart F; Osses N; Reyes JG
[Ad] Endereço:Instituto de Química, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
[Ti] Título:Arachidonic acid triggers [Ca2+]i increases in rat round spermatids by a likely GPR activation, ERK signalling and ER/acidic compartments Ca2+ release.
[So] Source:PLoS One;12(2):e0172128, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arachidonic acid (AA), a compound secreted by Sertoli cells (SC) in a FSH-dependent manner, is able to induce the release of Ca2+ from internal stores in round spermatids and pachytene spermatocytes. In this study, the possible site(s) of action of AA in round spermatids, the signalling pathways associated and the intracellular Ca2+ stores targeted by AA-induced signalling were pharmacologically characterized by measuring intracellular Ca2+ using fluorescent Ca2+ probes. Our results suggest that AA acts by interacting with a fatty acid G protein coupled receptor, initiating a G protein signalling cascade that may involve PLA2 and ERK activation, which in turn opens intracellular ryanodine-sensitive channels as well as NAADP-sensitive channels in acidic intracellular Ca2+ stores. The results presented here also suggest that AMPK and PKA modulate this AA-induced Ca2+ release from intracellular Ca2+ stores in round spermatids. We propose that unsaturated free fatty acid lipid signalling in the seminiferous tubule is a novel regulatory component of rat spermatogenesis.
[Mh] Termos MeSH primário: Ácido Araquidônico/farmacologia
Cálcio/metabolismo
Retículo Endoplasmático/efeitos dos fármacos
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Receptores Acoplados a Proteínas-G/agonistas
Espermátides/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Relação Dose-Resposta a Droga
Retículo Endoplasmático/metabolismo
Endossomos/efeitos dos fármacos
Endossomos/metabolismo
Cinética
Masculino
Microscopia Confocal
NADP/análogos & derivados
NADP/metabolismo
Fosfolipases A2/metabolismo
Ratos Sprague-Dawley
Receptores Acoplados a Proteínas-G/metabolismo
Salicilatos/farmacologia
Sesterterpenos/farmacologia
Espermátides/citologia
Espermátides/metabolismo
Testículo/citologia
Testículo/efeitos dos fármacos
Testículo/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (O3far1 protein, rat); 0 (Receptors, G-Protein-Coupled); 0 (Salicylates); 0 (Sesterterpenes); 0 (grifolic acid methyl ether); 0 (nicotinic acid adenine dinucleotide phosphate acetoxymethyl ester); 27YG812J1I (Arachidonic Acid); 53-59-8 (NADP); EC 3.1.1.4 (Phospholipases A2); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170214
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0172128


  5 / 291 MEDLINE  
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[PMID]:28112374
[Au] Autor:Morrison R; Lodge T; Evidente A; Kiss R; Townley H
[Ad] Endereço:Department of Engineering Science, University of Oxford, Oxford, UK.
[Ti] Título:Ophiobolin A, a sesterpenoid fungal phytotoxin, displays different mechanisms of cell death in mammalian cells depending upon the cancer cell origin.
[So] Source:Int J Oncol;50(3):773-786, 2017 Mar.
[Is] ISSN:1791-2423
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Herein we have undertaken a systematic analysis of the effects of the fungal derivative ophiobolin A (OphA) on eight cancer cell lines from different tissue types. The LD50 for each cell line was determined and the change in cell size determined. Flow cytometric analysis and western blotting were used to assess the cell death markers for early apoptosis, late apoptosis and necrosis, and the involvement of the caspase signalling pathway. Alterations in calcium levels and reactive oxygen species were assessed due to their integral involvement in intracellular signalling. Subsequently, the endoplasmic reticulum (ER) and mitochondrial responses were investigated more closely. The extent of ER swelling, and the upregulation of proteins involved in the unfolded protein responses (UPR) were seen to vary according to cell line. The mitochondria were also shown to behave differently in response to the OphA in the different cell lines in terms of the change in membrane potential, the total area of mitochondria in the cell and the number of mitochondrial bifurcations. The data obtained in the present study indicate that the cancer cell lines tested are unable to successfully activate the ER stress/UPR responses, and that the mitochondria appear to be a central player in OphA-induced cancer cell death.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Estresse do Retículo Endoplasmático/efeitos dos fármacos
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Micotoxinas/uso terapêutico
Sesterterpenos/uso terapêutico
[Mh] Termos MeSH secundário: Cálcio/metabolismo
Caspases/metabolismo
Linhagem Celular Tumoral
Retículo Endoplasmático/efeitos dos fármacos
Citometria de Fluxo
Células HeLa
Seres Humanos
Células MCF-7
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Poli(ADP-Ribose) Polimerases/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Transdução de Sinais/efeitos dos fármacos
Resposta a Proteínas não Dobradas/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mycotoxins); 0 (Reactive Oxygen Species); 0 (Sesterterpenes); 4611-05-6 (ophiobolin A); EC 2.4.2.30 (Poly(ADP-ribose) Polymerases); EC 3.4.22.- (Caspases); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170613
[Lr] Data última revisão:
170613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE
[do] DOI:10.3892/ijo.2017.3858


  6 / 291 MEDLINE  
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[PMID]:27820961
[Au] Autor:Fanelli F; Reveglia P; Masi M; Mulè G; Zonno MC; Cimmino A; Vurro M; Evidente A
[Ad] Endereço:a Institute of Sciences of Food Production , National Research Council , Bari , Italy.
[Ti] Título:Influence of light on the biosynthesis of ophiobolin A by Bipolaris maydis.
[So] Source:Nat Prod Res;31(8):909-917, 2017 Apr.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ophiobolin A (O-A) is a sesterpenoid with numerous biological activities, including potential anticancer effects. Its production at an industrial level is hampered due to inability of fungus Bipolaris maydis to biosynthesise it in vitro in large amount. Among the environmental factors regulating fungal metabolism, light plays a crucial role. In this study, the use of different light wavelength (light emitting diodes (LEDs)) was evaluated to increase the O-A production. The white light allowed the highest production of the metabolite. The blue and green lights showed an inhibitory effect, reducing the production to 50%, as well as red and yellow but at a lower level. No correlation between fungal growth and metabolite production was found in relation to the light type. A novel application of LED technologies, which can be optimised to foster specific pathways and promote the production of metabolites having scientific and industrial interest was proposed.
[Mh] Termos MeSH primário: Ascomicetos/metabolismo
Sesterterpenos/biossíntese
[Mh] Termos MeSH secundário: Microbiologia Industrial/métodos
Luz
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sesterterpenes); 4611-05-6 (ophiobolin A)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161109
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2016.1253084


  7 / 291 MEDLINE  
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[PMID]:27936075
[Au] Autor:Rodolfo C; Rocco M; Cattaneo L; Tartaglia M; Sassi M; Aducci P; Scaloni A; Camoni L; Marra M
[Ad] Endereço:Department of Biology, University of Rome Tor Vergata, Rome, Italy.
[Ti] Título:Ophiobolin A Induces Autophagy and Activates the Mitochondrial Pathway of Apoptosis in Human Melanoma Cells.
[So] Source:PLoS One;11(12):e0167672, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ophiobolin A, a fungal toxin from Bipolaris species known to affect different cellular processes in plants, has recently been shown to have anti-cancer activity in mammalian cells. In the present study, we investigated the anti-proliferative effect of Ophiobolin A on human melanoma A375 and CHL-1 cell lines. This cellular model was chosen because of the incidence of melanoma malignant tumor on human population and its resistance to chemical treatments. Ophyobolin A strongly reduced cell viability of melanoma cells by affecting mitochondrial functionality. The toxin induced depolarization of mitochondrial membrane potential, reactive oxygen species production and mitochondrial network fragmentation, leading to autophagy induction and ultimately resulting in cell death by activation of the mitochondrial pathway of apoptosis. Finally, a comparative proteomic investigation on A375 cells allowed to identify several Ophiobolin A down-regulated proteins, which are involved in fundamental processes for cell homeostasis and viability.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Apoptose/efeitos dos fármacos
Melanoma/tratamento farmacológico
Mitocôndrias/efeitos dos fármacos
Sesterterpenos/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/química
Ascomicetos/química
Autofagia/efeitos dos fármacos
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Seres Humanos
Melanoma/metabolismo
Melanoma/patologia
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Mitocôndrias/metabolismo
Mitocôndrias/patologia
Espécies Reativas de Oxigênio/metabolismo
Sesterterpenos/química
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Reactive Oxygen Species); 0 (Sesterterpenes); 4611-05-6 (ophiobolin A)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170707
[Lr] Data última revisão:
170707
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0167672


  8 / 291 MEDLINE  
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[PMID]:27725502
[Au] Autor:Kawakami S; Inagaki M; Matsunami K; Otsuka H; Kawahata M; Yamaguchi K
[Ad] Endereço:Department of Natural Product Chemistry, Faculty of Pharmacy, Yasuda Women's University.
[Ti] Título:Crotofolane-Type Diterpenoids, Crotocascarins L-Q, and a Rearranged Crotofolane-Type Diterpenoid, Neocrotocascarin, from the Stems of Croton cascarilloides.
[So] Source:Chem Pharm Bull (Tokyo);64(10):1492-1498, 2016.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:From the stems of Croton cascarilloides, further crotofolane-type diterpenoids, named crotocascarins L-Q (1-6), and a rearranged one (7), named neocrotocascarin were isolated. Their structures were elucidated from spectroscopic evidence. Also, the structures of crotocascarin O (4) and neocrotocascarin (7) were determined by X-ray crystallographic analyses. As a result, neocrotocascarin (7) was found to possess a new carbon framework. A plausible mechanism for the formation of neocrotocascarin (7) is also discussed.
[Mh] Termos MeSH primário: Croton/química
Diterpenos/química
Diterpenos/isolamento & purificação
Caules de Planta/química
Sesterterpenos/isolamento & purificação
[Mh] Termos MeSH secundário: Cristalografia por Raios X
Modelos Moleculares
Conformação Molecular
Sesterterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Diterpenes); 0 (Sesterterpenes)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170131
[Lr] Data última revisão:
170131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161012
[St] Status:MEDLINE


  9 / 291 MEDLINE  
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[PMID]:27403889
[Au] Autor:Chidley C; Trauger SA; Birsoy K; O'Shea EK
[Ad] Endereço:Faculty of Arts and Sciences Center for Systems Biology, Harvard University, Cambridge, United States.
[Ti] Título:The anticancer natural product ophiobolin A induces cytotoxicity by covalent modification of phosphatidylethanolamine.
[So] Source:Elife;5, 2016 Jul 12.
[Is] ISSN:2050-084X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phenotypic screens allow the identification of small molecules with promising anticancer activity, but the difficulty in characterizing the mechanism of action of these compounds in human cells often undermines their value as drug leads. Here, we used a loss-of-function genetic screen in human haploid KBM7 cells to discover the mechanism of action of the anticancer natural product ophiobolin A (OPA). We found that genetic inactivation of de novo synthesis of phosphatidylethanolamine (PE) mitigates OPA cytotoxicity by reducing cellular PE levels. OPA reacts with the ethanolamine head group of PE in human cells to form pyrrole-containing covalent cytotoxic adducts and these adducts lead to lipid bilayer destabilization. Our characterization of this unusual cytotoxicity mechanism, made possible by unbiased genetic screening in human cells, suggests that the selective antitumor activity displayed by OPA may be due to altered membrane PE levels in cancer cells.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Produtos Biológicos/farmacologia
Fosfatidiletanolaminas/metabolismo
Sesterterpenos/farmacologia
[Mh] Termos MeSH secundário: Linhagem Celular
Membrana Celular/efeitos dos fármacos
Seres Humanos
Bicamadas Lipídicas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Biological Products); 0 (Lipid Bilayers); 0 (Phosphatidylethanolamines); 0 (Sesterterpenes); 39382-08-6 (phosphatidylethanolamine); 4611-05-6 (ophiobolin A)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160713
[St] Status:MEDLINE


  10 / 291 MEDLINE  
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[PMID]:27399730
[Au] Autor:Elhady SS; Al-Abd AM; El-Halawany AM; Alahdal AM; Hassanean HA; Ahmed SA
[Ad] Endereço:Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt. ssahmed@kau.edu.sa.
[Ti] Título:Antiproliferative Scalarane-Based Metabolites from the Red Sea Sponge Hyrtios erectus.
[So] Source:Mar Drugs;14(7), 2016 Jul 08.
[Is] ISSN:1660-3397
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Two new sesterterpenes analogs, namely, 12-acetoxy,16-epi-hyrtiolide (1) and 12ß-acetoxy,16ß-methoxy,20α-hydroxy-17-scalaren-19,20-olide (2), containing a scalarane-based framework along with seven previously reported scalarane-type sesterterpenes (3-9) have been isolated from the sponge Hyrtios erectus (order Dictyoceratida) collected from the Red Sea, Egypt. The structures of the isolated compounds were elucidated on the basis of their spectroscopic data and comparison with reported NMR data. Compounds 1-9 exhibited considerable antiproliferative activity against breast adenocarcinoma (MCF-7), colorectal carcinoma (HCT-116) and hepatocellular carcinoma cells (HepG2). Compounds 3, 5 and 9 were selected for subsequent investigations regarding their mechanism of cell death induction (differential apoptosis/necrosis assessment) and their influence on cell cycle distribution.
[Mh] Termos MeSH primário: Proliferação Celular/efeitos dos fármacos
Poríferos/química
Sesterterpenos/química
Sesterterpenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Neoplasias da Mama/tratamento farmacológico
Ciclo Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Neoplasias Colorretais/tratamento farmacológico
Egito
Feminino
Células HCT116
Células Hep G2
Seres Humanos
Neoplasias Hepáticas/tratamento farmacológico
Células MCF-7
Espectroscopia de Ressonância Magnética/métodos
Estrutura Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sesterterpenes)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170510
[Lr] Data última revisão:
170510
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160712
[St] Status:MEDLINE



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