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[PMID]:25025226
[Au] Autor:Liu Z; Szarecka A; Yonkunas M; Speranskiy K; Kurnikova M; Cascio M
[Ad] Endereço:Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
[Ti] Título:Crosslinking constraints and computational models as complementary tools in modeling the extracellular domain of the glycine receptor.
[So] Source:PLoS One;9(7):e102571, 2014.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The glycine receptor (GlyR), a member of the pentameric ligand-gated ion channel superfamily, is the major inhibitory neurotransmitter-gated receptor in the spinal cord and brainstem. In these receptors, the extracellular domain binds agonists, antagonists and various other modulatory ligands that act allosterically to modulate receptor function. The structures of homologous receptors and binding proteins provide templates for modeling of the ligand-binding domain of GlyR, but limitations in sequence homology and structure resolution impact on modeling studies. The determination of distance constraints via chemical crosslinking studies coupled with mass spectrometry can provide additional structural information to aid in model refinement, however it is critical to be able to distinguish between intra- and inter-subunit constraints. In this report we model the structure of GlyBP, a structural and functional homolog of the extracellular domain of human homomeric α1 GlyR. We then show that intra- and intersubunit Lys-Lys crosslinks in trypsinized samples of purified monomeric and oligomeric protein bands from SDS-polyacrylamide gels may be identified and differentiated by MALDI-TOF MS studies of limited resolution. Thus, broadly available MS platforms are capable of providing distance constraints that may be utilized in characterizing large complexes that may be less amenable to NMR and crystallographic studies. Systematic studies of state-dependent chemical crosslinking and mass spectrometric identification of crosslinked sites has the potential to complement computational modeling efforts by providing constraints that can validate and refine allosteric models.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Receptores da Glicina/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Reagentes para Ligações Cruzadas/química
Dimetil Suberimidato/química
Ligações de Hidrogênio
Simulação de Dinâmica Molecular
Dados de Sequência Molecular
Ligação Proteica
Domínios e Motivos de Interação entre Proteínas
Subunidades Proteicas/química
Células Sf9
Spodoptera
Homologia Estrutural de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Cross-Linking Reagents); 0 (Protein Subunits); 0 (Receptors, Glycine); 29878-26-0 (Dimethyl Suberimidate)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140716
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0102571


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[PMID]:24781457
[Au] Autor:Koolen HH; Gomes AF; Schwab NV; Eberlin MN; Gozzo FC
[Ad] Endereço:Institute of Chemistry, University of Campinas and Instituto Nacional de Ciência e Tecnologia de Bioanalítica, Sao Paulo, 13083-970, Brazil.
[Ti] Título:Imidate-based cross-linkers for structural proteomics: increased charge of protein and peptide ions and CID and ECD fragmentation studies.
[So] Source:J Am Soc Mass Spectrom;25(7):1181-91, 2014 Jul.
[Is] ISSN:1879-1123
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chemical cross-linking is an attractive low-resolution technique for structural studies of protein complexes. Distance constraints obtained from cross-linked peptides identified by mass spectrometry (MS) are used to construct and validate protein models. Amidinating cross-linkers such as diethyl suberthioimidate (DEST) have been used successfully in chemical cross-linking experiments. In this work, the application of a commercial diimidate cross-linking reagent, dimethyl suberimidate (DMS), was evaluated with model peptides and proteins. The peptides were designed with acetylated N-termini followed by random sequences containing two Lys residues separated by an Arg residue. After cross-linking reactions, intra- and intermolecular cross-linked species were submitted to CID and ECD dissociations to study their fragmentation features in the gas phase. Fragmentation of intramolecular peptides by collision induced dissociation (CID) demonstrates a unique two-step fragmentation pathway involving formation of a ketimine as intermediate. Electron capture and electron transfer dissociation (ECD and ETD) experiments demonstrated that the cyclic moiety is not dissociated. Intermolecular species demonstrated previously described fragmentation behavior in both CID and ECD experiments. The charge state distributions (CSD) obtained after reaction with DMS were compared with those obtained with disuccinimidyl suberate (DSS). CSDs for peptides and proteins were increased after their reaction with DMS, owing to the higher basicity of DMS modified species. These features were also observed in LC-MS experiments with bovine carbonic anhydrase II (BCA) after cross-linking with DMS and tryptic proteolysis. Cross-linked peptides derived from this protein were identified at high confidence and those species were in agreement with the crystal structure of BCA.
[Mh] Termos MeSH primário: Reagentes para Ligações Cruzadas/química
Dimetil Suberimidato/química
Peptídeos/química
Proteínas/química
Proteômica/métodos
[Mh] Termos MeSH secundário: Animais
Anidrase Carbônica II/química
Bovinos
Modelos Moleculares
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 0 (Peptides); 0 (Proteins); 29878-26-0 (Dimethyl Suberimidate); EC 4.2.1.- (Carbonic Anhydrase II)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140501
[St] Status:MEDLINE
[do] DOI:10.1007/s13361-014-0900-5


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[PMID]:23063560
[Au] Autor:Rowell JP; Simpson KL; Stott K; Watson M; Thomas JO
[Ad] Endereço:Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK.
[Ti] Título:HMGB1-facilitated p53 DNA binding occurs via HMG-Box/p53 transactivation domain interaction, regulated by the acidic tail.
[So] Source:Structure;20(12):2014-24, 2012 Dec 05.
[Is] ISSN:1878-4186
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Facilitated binding of p53 to DNA by high mobility group B1 (HMGB1) may involve interaction between the N-terminal region of p53 and the high mobility group (HMG) boxes, as well as HMG-induced bending of the DNA. Intramolecular shielding of the boxes by the HMGB1 acidic tail results in an unstable complex with p53 until the tail is truncated to half its length, at which point the A box, proposed to be the preferred binding site for p53(1-93), is exposed, leaving the B box to bind and bend DNA. The A box interacts with residues 38-61 (TAD2) of the p53 transactivation domain. Residues 19-26 (TAD1) bind weakly, but only in the context of p53(1-93) and not as a free TAD1 peptide. We have solved the structure of the A-box/p53(1-93) complex by nuclear magnetic resonance spectroscopy. The incipient amphipathic helix in TAD2 recognizes the concave DNA-binding face of the A box and may be acting as a single-stranded DNA mimic.
[Mh] Termos MeSH primário: Proteína HMGB1/química
Proteína Supressora de Tumor p53/química
[Mh] Termos MeSH secundário: Cromatografia em Gel
Reagentes para Ligações Cruzadas/química
Dimetil Suberimidato/química
Domínios HMG-Box
Proteína HMGB1/isolamento & purificação
Seres Humanos
Modelos Moleculares
Mimetismo Molecular
Fragmentos de Peptídeos/química
Fragmentos de Peptídeos/isolamento & purificação
Ligação Proteica
Domínios e Motivos de Interação entre Proteínas
Mapeamento de Interação de Proteínas
Estrutura Secundária de Proteína
Proteína Supressora de Tumor p53/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 0 (HMGB1 Protein); 0 (Peptide Fragments); 0 (TP53 protein, human); 0 (Tumor Suppressor Protein p53); 29878-26-0 (Dimethyl Suberimidate)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:121211
[Lr] Data última revisão:
121211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121016
[St] Status:MEDLINE


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[PMID]:23038665
[Au] Autor:Frosini M; Larini A; Ricci L; Lucas L; Gorelli B; Sgaragli G; Tanganelli P; Valoti M
[Ad] Endereço:Dipartimento di Neuroscienze, Sezione di Farmacologia, Università degli Studi di Siena, Siena, Italy. frosinim@unisi.it
[Ti] Título:Effects of autologous, cross-linked erythrocytes on isolated hypoperfused rabbit heart dynamics.
[So] Source:Pharmacology;90(5-6):274-80, 2012.
[Is] ISSN:1423-0313
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The present study was aimed at assessing the effects of either red blood cells (RBC) or RBC cross-linked with the bifunctional dimethyl suberimidate reagent (C-RBC) on contractile force (CFo), heart rate (HR) and coronary flow (CF) of the isolated rabbit heart hypoperfused with RBC suspensions under 30 mm Hg constant pressure. RBC or C-RBC caused a rapid and marked reduction of CF, CFo and HR. In RBC-treated hearts, however, reperfusion with Tyrode solution partially restored the initial myocardial parameters, while in C-RBC-treated hearts a rapid impairment of diastolic relaxation with a subsequent, steady and increasing heart contracture was observed. Histological analysis showed that in C-RBC-perfused hearts either capillaries or precapillary arterioles were occluded by C-RBC in spite of extensive washings with Tyrode solution. These findings indicate that C-RBC impair coronary circulation markedly and irreversibly.
[Mh] Termos MeSH primário: Reagentes para Ligações Cruzadas/farmacologia
Dimetil Suberimidato/farmacologia
Eritrócitos
Coração/efeitos dos fármacos
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/metabolismo
Animais
Circulação Coronária/efeitos dos fármacos
Coração/fisiologia
Frequência Cardíaca/efeitos dos fármacos
Técnicas In Vitro
Masculino
Contração Miocárdica/efeitos dos fármacos
Perfusão
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 29878-26-0 (Dimethyl Suberimidate); 8L70Q75FXE (Adenosine Triphosphate)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:141120
[Lr] Data última revisão:
141120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121006
[St] Status:MEDLINE
[do] DOI:10.1159/000341910


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[PMID]:18727399
[Au] Autor:Kalacheva NV; Konovalova OA; Nalimov DS; Salakhov MKh; Il'inskaia ON; Kurinenko BM
[Ti] Título:[Inhibition of functional activity of macrophages in vitro by dimer RNase Bacillus intermedius].
[So] Source:Tsitologiia;50(6):487-91, 2008.
[Is] ISSN:0041-3771
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The influence of cross-linked by dimethylsuberimidate dimeric RNAse from Bacillus intermedius on peritoneal rat macrophages has been investigated in vitro. It has been shown that dimeric RNase with concentrations of 0.5-40.0 mg/ml decreases the functional activities of macrophages. This is manifested in the inhibition of the phagocyte function of macrophages and suppression of the fusion of phagosomes with lysosomes. The change in the cytoplasmatic membrane surface structure induced by the dimers, which is stronger than that induced by monomers, has been demonstrated using atomic force microscopy. The role of membrane properties modification in the inhibition effect of RNase dimers on the functional activities of macrophages is discussed.
[Mh] Termos MeSH primário: Bacillus/enzimologia
Proteínas de Bactérias/farmacologia
Macrófagos Peritoneais/efeitos dos fármacos
Fagocitose/efeitos dos fármacos
Ribonucleases/farmacologia
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/química
Dimerização
Dimetil Suberimidato/química
Lisossomos/imunologia
Macrófagos Peritoneais/imunologia
Macrófagos Peritoneais/ultraestrutura
Microscopia de Força Atômica
Fagossomos/imunologia
Ratos
Ribonucleases/química
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 29878-26-0 (Dimethyl Suberimidate); EC 3.1.- (Ribonucleases)
[Em] Mês de entrada:0810
[Cu] Atualização por classe:080827
[Lr] Data última revisão:
080827
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080830
[St] Status:MEDLINE


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[PMID]:18004715
[Au] Autor:Matyka K; Matyka M; Mróz I; Zalewska-Rejdak J; Ciszewski A
[Ad] Endereço:Institute of Experimental Physics, University of Wroclaw, pl. Maxa Borna 9, 50-204 Wroclaw, Poland. lamia@ifd.uni.wroc.pl
[Ti] Título:An AFM study on mechanical properties of native and dimethyl suberimidate cross-linked pericardium tissue.
[So] Source:J Mol Recognit;20(6):524-30, 2007 Nov-Dec.
[Is] ISSN:0952-3499
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Changes in the stiffness of hog pericardium tissue, native and treated with dimethyl suberimidate (DMS), are investigated by atomic force microscopy (AFM). Young's modulus is calculated on the basis of the Hertz-Sneddon model. The cross-linking process increases the stiffness of the tissue. The values of Young's modulus are higher for the DMS stabilized pericardium than for the native one. We also observe that the Young's modulus of native tissue increases when the time between getting the biological material and performing the measurements is longer. This process is probably connected with natural degradation of the biological samples.
[Mh] Termos MeSH primário: Dimetil Suberimidato/farmacologia
Microscopia de Força Atômica
Pericárdio/efeitos dos fármacos
Pericárdio/ultraestrutura
[Mh] Termos MeSH secundário: Animais
Fenômenos Biomecânicos
Reagentes para Ligações Cruzadas/farmacologia
Processamento de Imagem Assistida por Computador
Pericárdio/química
Propriedades de Superfície
Suínos
Fatores de Tempo
Preservação de Tecido/métodos
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 29878-26-0 (Dimethyl Suberimidate)
[Em] Mês de entrada:0805
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:071116
[St] Status:MEDLINE


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[PMID]:12359075
[Au] Autor:Nishino T; Amaya Y; Kawamoto S; Kashima Y; Okamoto K; Nishino T
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Nippon Medical School, Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan. nishino@nms.ac.jp
[Ti] Título:Purification and characterization of multiple forms of rat liver xanthine oxidoreductase expressed in baculovirus-insect cell system.
[So] Source:J Biochem;132(4):597-606, 2002 Oct.
[Is] ISSN:0021-924X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:cDNA of rat liver xanthine oxidoreductase (XOR), a molybdenum-containing iron-sulfur flavoprotein, was expressed in a baculovirus-insect cell system. The expressed XOR consisted of a heterogeneous mixture of native dimeric, demolybdo-dimeric, and monomeric forms, each of which was separated and purified to homogeneity. All the expressed forms contained flavin, of which the semiquinone form was stable during dithionite titration after dithiothreitol treatment, indicating that the flavin domains of all the expressed molecules have the intact conformations interconvertible between NAD(+)-dependent dehydrogenase (XDH) and O(2)-dependent oxidase (XO) types. The absorption spectrum and metal analyses showed that the monomeric form lacks not only molybdopterin but also one of the iron-sulfur centers. The reductive titration of the monomer with dithionite showed that the monomeric form required only three electrons for complete reduction, and the redox potential of the iron-sulfur center in the monomeric form is a lower value than that of FAD. In contrast to native or demolybdo-dimeric XDHs, the monomer showed a very slow reductive process with NADH under anaerobic conditions, although the conformation around FAD is a dehydrogenase form, suggesting the important role of the iron-sulfur center in the reductive process of FAD with the reduced pyridine nucleotide.
[Mh] Termos MeSH primário: Proteínas com Ferro-Enxofre/química
Fígado/enzimologia
Xantina Desidrogenase/química
Xantina Oxidase/química
[Mh] Termos MeSH secundário: Animais
Baculoviridae/genética
Bovinos
Linhagem Celular
Reagentes para Ligações Cruzadas/química
Dimerização
Dimetil Suberimidato/química
Proteínas com Ferro-Enxofre/isolamento & purificação
Leite/enzimologia
Molibdênio/química
Oxirredução
Ratos
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/isolamento & purificação
Espectrofotometria
Spodoptera/virologia
Xantina Desidrogenase/biossíntese
Xantina Desidrogenase/genética
Xantina Desidrogenase/isolamento & purificação
Xantina Oxidase/biossíntese
Xantina Oxidase/genética
Xantina Oxidase/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 0 (Iron-Sulfur Proteins); 0 (Recombinant Proteins); 29878-26-0 (Dimethyl Suberimidate); 81AH48963U (Molybdenum); EC 1.17.1.4 (Xanthine Dehydrogenase); EC 1.17.3.2 (Xanthine Oxidase)
[Em] Mês de entrada:0306
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:021003
[St] Status:MEDLINE


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[PMID]:11906824
[Au] Autor:Ogawa H; Gomi T; Takusagawa F; Masuda T; Goto T; Kan T; Huh NH
[Ad] Endereço:Faculty of Medicine, Department of Biochemistry, Toyama Medical and Pharmaceutical University, Toyama 930-0194, Japan. hogawa@ms.toyama-mpu.ac.jp
[Ti] Título:Evidence for a dimeric structure of rat liver serine dehydratase.
[So] Source:Int J Biochem Cell Biol;34(5):533-43, 2002 May.
[Is] ISSN:1357-2725
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Rat liver serine dehydratase (SDH) is known to be involved in gluconeogenesis. It has long been believed to be a dimeric protein with the subunit molecular weight (M(r)) of 34,000. Recently, sheep liver SDH was reported to be a monomer with a M(r) of 38,000. The native M(r) of rat SDH was only determined by the ultracentrifugation method more than three decades ago, and that of sheep SDH was done by the method of gel chromatography. The primary to quaternary structures of a given enzyme in a specific mammalian organ are usually conserved among various species. The aim of the present investigation is to clarify the structural differences between rat and sheep SDHs. First, we found that the amino acid composition reported for sheep SDH was statistically similar to that of rat SDH. Second, immunoblot analysis using anti-rat SDH IgG as the probe showed the size of sheep SDH to be a M(r) of 30,500, whereas that of SDH was about M(r) of 35,000. On the other hand, the native size of rat SDH was assessed by two methods: (1) the laser light scattering method demonstrated that rat SDH had a M(r) of 66,800, consistent with the previous value (M(r)=64,000); (2) cross-linking experiments of the purified rat SDH with dimethyl suberimidate revealed the existence of a dimeric form by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The present results clearly confirm that rat SDH is a dimer, and suggest that sheep SDH is similar to rat SDH immunologically, but with a molecular weight 7500 smaller than reported previously.
[Mh] Termos MeSH primário: L-Serina Desidratase/química
Fígado/enzimologia
[Mh] Termos MeSH secundário: Aminoácidos/análise
Animais
Cromatografia em Gel
Reagentes para Ligações Cruzadas/química
Dimerização
Dimetil Suberimidato/química
Seres Humanos
L-Serina Desidratase/isolamento & purificação
L-Serina Desidratase/metabolismo
Lasers
Masculino
Peso Molecular
Estrutura Quaternária de Proteína
Ratos
Ratos Sprague-Dawley
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Amino Acids); 0 (Cross-Linking Reagents); 29878-26-0 (Dimethyl Suberimidate); EC 4.3.1.17 (L-Serine Dehydratase)
[Em] Mês de entrada:0206
[Cu] Atualização por classe:071114
[Lr] Data última revisão:
071114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:020322
[St] Status:MEDLINE


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[PMID]:11878811
[Au] Autor:Chen JC; von Lintig FC; Jones SB; Huvar I; Boss GR
[Ad] Endereço:Department of Medicine and Cancer Center, University of California at San Diego, La Jolla, California 92093-0652, USA.
[Ti] Título:High-efficiency solid-phase capture using glass beads bonded to microcentrifuge tubes: immunoprecipitation of proteins from cell extracts and assessment of ras activation.
[So] Source:Anal Biochem;302(2):298-304, 2002 Mar 15.
[Is] ISSN:0003-2697
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We have bonded glass microbeads (425-600 microm diameter) to the inner walls of polypropylene microcentrifuge tubes. In addition to increasing the surface area of the tubes manyfold, the beads provide surface Si groups which can be reacted with a silane compound such as aminopropyltriethoxysilane, yielding a free amino group. The amino group is reacted with another cross-linking reagent, for example, the homobifunctional compound dimethyl suberimidate, which can form a covalent bond with amine groups of proteins. After binding protein A or G to the dimethyl suberimidate, the beads were used to immunoprecipitate proteins from cell extracts; we show that the protein A/G-coated glass beads yield similar amounts of immunoprecipitated proteins as a standard method using protein A- or G-agarose beads, but with fewer contaminating proteins. In addition, we show that when immunoprecipitating Ras from cell extracts and measuring the amounts of Ras-bound GTP and GDP, the new method yielded higher guanine nucleotide levels than protein G-agarose beads, suggesting that it caused less denaturation of Ras. Because the glass beads are bonded to the walls of the tubes, the immunoprecipitates can be washed rapidly and efficiently, and we show that 20-30 tubes can be washed in 1/10 the time required to wash immunoprecipitates on protein A- or G-agarose beads.
[Mh] Termos MeSH primário: Extratos Celulares/química
Dimetil Suberimidato/química
Inibidores de Dissociação do Nucleotídeo Guanina/análise
Proteína Estafilocócica A/química
Proteínas ras/análise
[Mh] Termos MeSH secundário: Células 3T3/química
Animais
Inibidores de Dissociação do Nucleotídeo Guanina/imunologia
Células HL-60/química
Seres Humanos
Camundongos
Microesferas
Polipropilenos/química
Testes de Precipitina/métodos
Proteínas/análise
Proteínas/imunologia
Silanos/química
Células Tumorais Cultivadas
Proteínas ras/imunologia
Inibidores da Dissociação do Nucleotídeo Guanina rho-Específico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Nm] Nome de substância:
0 (Cell Extracts); 0 (Guanine Nucleotide Dissociation Inhibitors); 0 (Polypropylenes); 0 (Proteins); 0 (Silanes); 0 (Staphylococcal Protein A); 0 (rho-Specific Guanine Nucleotide Dissociation Inhibitors); 29878-26-0 (Dimethyl Suberimidate); EC 3.6.5.2 (ras Proteins)
[Em] Mês de entrada:0206
[Cu] Atualização por classe:121115
[Lr] Data última revisão:
121115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:020307
[St] Status:MEDLINE


  10 / 310 MEDLINE  
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Fotocópia
[PMID]:11606252
[Au] Autor:Moshnikova AB; Moshnikov SA; Afanasyev VN; Krotova KE; Sadovnikov VB; Beletsky IP
[Ad] Endereço:Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
[Ti] Título:Cell death induced by chemical homobifunctional cross-linkers. Cross-linker induced apoptosis.
[So] Source:Int J Biochem Cell Biol;33(12):1160-71, 2001 Dec.
[Is] ISSN:1357-2725
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Physical association of proteins that underlies cytotoxic signal induction and transduction suggests a possibility of regulating cell response by modifying protein-protein interactions. For protein complexing, chemical cross-linking agents have been traditionally used. However, the ability of various cross-linkers to induce and modify cell responses, cell death in particular, is still obscure. We have undertaken the investigation to test the apoptosis-inducing and modifying properties of the homobifunctional cross-linkers-dimethyl suberimidate (DMS) and 1,5-bis(succinimido-oxycarbonyloxy)pentane (BSOCOP). The functional groups of these cross-linkers are different but both are able to interact with available amino groups. It was shown that bifunctional cross-linkers, unlike their monofunctional analogues, are capable of inducing cell death in transformed cells, thus indicating the crucial role of cross-linking in cell killing. DMS- and BSOCOP-treated cells were shown to undergo cell death by apoptosis, though the signaling pathways were distinct. DMS inhibited bcl-X(L) and bak but not bax gene expression, while BSOCOP potentiated bax mRNA synthesis immediately after application. Cell pre-incubation with DMS, but not with BSOCOP, resulted in an increasing sensitivity to TNF, although activities of anti-Fas cytotoxic antibodies were then inhibited. Thus, this study has demonstrated for the first time that chemical cross-linkers are capable of inducing apoptosis by themselves and modifying the TNF-dependent and Fas-mediated cell death that may have potential therapeutic significance.
[Mh] Termos MeSH primário: Apoptose
Reagentes para Ligações Cruzadas/farmacologia
[Mh] Termos MeSH secundário: Animais
Morte Celular
Linhagem Celular
Dimetil Suberimidato/farmacologia
Relação Dose-Resposta a Droga
Células HeLa
Seres Humanos
Proteínas de Membrana/biossíntese
Camundongos
Pentanos/farmacologia
Proteínas Proto-Oncogênicas/biossíntese
Proteínas Proto-Oncogênicas c-bcl-2/biossíntese
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Transdução de Sinais
Fatores de Tempo
Células Tumorais Cultivadas
Fator de Necrose Tumoral alfa/metabolismo
Células U937
Proteína Killer-Antagonista Homóloga a bcl-2
Proteína X Associada a bcl-2
Proteína bcl-X
Receptor fas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (1,5-bis(succinimido-oxycarbonyloxy)pentane); 0 (BAK1 protein, human); 0 (BAX protein, human); 0 (BCL2L1 protein, human); 0 (Bak1 protein, mouse); 0 (Bax protein, mouse); 0 (Bcl2l1 protein, mouse); 0 (Cross-Linking Reagents); 0 (Membrane Proteins); 0 (Pentanes); 0 (Proto-Oncogene Proteins); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (RNA, Messenger); 0 (Tumor Necrosis Factor-alpha); 0 (bcl-2 Homologous Antagonist-Killer Protein); 0 (bcl-2-Associated X Protein); 0 (bcl-X Protein); 0 (fas Receptor); 29878-26-0 (Dimethyl Suberimidate)
[Em] Mês de entrada:0201
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:011019
[St] Status:MEDLINE



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