Base de dados : MEDLINE
Pesquisa : D02.491.203.425 [Categoria DeCS]
Referências encontradas : 607 [refinar]
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[PMID]:29229149
[Au] Autor:Ding J; Liu S; Zhang D; Song Y; Ma X; Yi C; Song B; Xiao B; Su Y; Guo S
[Ad] Endereço:Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, China.
[Ti] Título:Transfusion of ethylene carbodiimide-fixed donor splenocytes prolongs survival of vascularized skin allografts.
[So] Source:J Surg Res;221:343-352, 2018 Jan.
[Is] ISSN:1095-8673
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Allograft rejection is a major obstacle to the widespread clinical application of vascularized composite allotransplantation. Recent studies revealed a noncytoreductive strategy to protect allografts by the transfusion of ethylene carbodiimide-fixed donor splenocytes (ECDI-SPs). To determine whether this approach offers advantages in protecting skin allografts, we examined the immunological protection of infusing ECDI-SPs with a 30-d administration of rapamycin on the skin allografts of mice. MATERIALS AND METHODS: C57BL/6 recipient mice received BALB/c donor full-thickness skin or vascularized skin transplants at day 0, along with the infusion of donor ECDI-SPs 7 d before and 1 d after allotransplantation and a 30-d course of rapamycin. Recipients received ECDI-untreated splenocytes or C3H allografts as controls. In vitro allostimulatory activity of ECDI-SPs and donor-specific ex vivo hyporesponsiveness were tested. Production of related cytokines (TGF-ß, IL-10, IL-1ß, and TNF-α) and expression of CD4 Foxp3 regulatory T cells (Tregs) were also examined. RESULTS: Transfusion of ECDI-SPs combined with rapamycin significantly prolonged survival of full-thickness skin (median survival time [MST]: 28 d) and full-thickness skin allografts (MST: 71 d) compared with untreated splenocytes (MSTs: 11 d and 30 d) or C3H allografts (MSTs: 11 d and 38 d). This effect was accompanied by increased production of IL-10 and TGF-ß, decreased production of IL-1ß and TNF-α, and expansion of Tregs in vitro and in vivo. CONCLUSIONS: ECDI-SP infusion combined with short-term rapamycin administration provides a promising approach to prolong the skin allograft survival.
[Mh] Termos MeSH primário: Transplante de Células/métodos
Rejeição de Enxerto/prevenção & controle
Imunossupressores/administração & dosagem
Sirolimo/administração & dosagem
Transplante de Pele
[Mh] Termos MeSH secundário: Animais
Citocinas/metabolismo
Etildimetilaminopropil Carbodi-Imida
Rejeição de Enxerto/imunologia
Masculino
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Baço/citologia
Linfócitos T Reguladores
Transplante Homólogo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Immunosuppressive Agents); RJ5OZG6I4A (Ethyldimethylaminopropyl Carbodiimide); W36ZG6FT64 (Sirolimus)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE


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[PMID]:27915017
[Au] Autor:Bax DV; Davidenko N; Gullberg D; Hamaia SW; Farndale RW; Best SM; Cameron RE
[Ad] Endereço:Department of Materials Science and Metallurgy, University of Cambridge, 27 Charles Babbage Road, Cambridge CB3 0FS, United Kingdom; Department of Biochemistry, University of Cambridge, Downing Site, Cambridge CB2 1QW, United Kingdom. Electronic address: dvb24@cam.ac.uk.
[Ti] Título:Fundamental insight into the effect of carbodiimide crosslinking on cellular recognition of collagen-based scaffolds.
[So] Source:Acta Biomater;49:218-234, 2017 Feb.
[Is] ISSN:1878-7568
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Research on the development of collagen constructs is extremely important in the field of tissue engineering. Collagen scaffolds for numerous tissue engineering applications are frequently crosslinked with 1-ethyl-3-(3-dimethylaminopropyl-carbodiimide hydrochloride (EDC) in the presence of N-hydroxy-succinimide (NHS). Despite producing scaffolds with good biocompatibility and low cellular toxicity the influence of EDC/NHS crosslinking on the cell interactive properties of collagen has been overlooked. Here we have extensively studied the interaction of model cell lines with collagen I-based materials after crosslinking with different ratios of EDC in relation to the number of carboxylic acid residues on collagen. Divalent cation-dependent cell adhesion, via integrins α ß , α ß , α ß and α ß , were sensitive to EDC crosslinking. With increasing EDC concentration, this was replaced with cation-independent adhesion. These results were replicated using purified recombinant I domains derived from integrin α and α subunits. Integrin α ß -mediated cell spreading, apoptosis and proliferation were all heavily influenced by EDC crosslinking of collagen. Data from this rigorous study provides an exciting new insight that EDC/NHS crosslinking is utilising the same carboxylic side chain chemistry that is vital for native-like integrin-mediated cell interactions. Due to the ubiquitous usage of EDC/NHS crosslinked collagen for biomaterials fabrication this data is essential to have a full understanding in order to ensure optimized collagen-based material performance. STATEMENT OF SIGNIFICANCE: Carbodiimide stabilised collagen is employed extensively for the fabrication of biologically active materials. Despite this common usage, the effect of carbodiimide crosslinking on cell-collagen interactions is unclear. Here we have found that carbodiimide crosslinking of collagen inhibits native-like, whilst increasing non-native like, cellular interactions. We propose a mechanistic model in which carbodiimide modifies the carboxylic acid groups on collagen that are essential for cell binding. As such we feel that this research provides a crucial, long awaited, insight into the bioactivity of carbodiimide crosslinked collagen. Through the ubiquitous use of collagen as a cellular substrate we feel that this is fundamental to a wide range of research activity with high impact across a broad range of disciplines.
[Mh] Termos MeSH primário: Colágeno/química
Reagentes para Ligações Cruzadas/química
Etildimetilaminopropil Carbodi-Imida/química
Tecidos Suporte/química
[Mh] Termos MeSH secundário: Animais
Cátions
Bovinos
Adesão Celular
Linhagem Celular
Proliferação Celular
Sobrevivência Celular
Seres Humanos
Integrina alfa2beta1/metabolismo
Camundongos
Domínios Proteicos
Solubilidade
Succinimidas
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cations); 0 (Cross-Linking Reagents); 0 (Integrin alpha2beta1); 0 (Succinimides); 9007-34-5 (Collagen); MJE3791M4T (N-hydroxysuccinimide); RJ5OZG6I4A (Ethyldimethylaminopropyl Carbodiimide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161205
[St] Status:MEDLINE


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[PMID]:27383886
[Au] Autor:Thanyaphoo S; Kaewsrichan J
[Ti] Título:A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2.
[So] Source:Acta Pharm;66(3):373-85, 2016 Sep 01.
[Is] ISSN:1846-9558
[Cp] País de publicação:Croatia
[La] Idioma:eng
[Ab] Resumo:Silicon-substituted calcium phosphate (Si-CaP) was developed in our laboratory as a biomaterial for delivery in bone tissue engineering. It was fabricated as a 3D-construct of scaffolds using chitosan-trisodium polyphosphate (TPP) cross-linked networks. In this study, heparin was covalently bonded to the residual -NH2 groups of chitosan on the scaffold applying carbodiimide chemistry. Bonded heparin was not leached away from scaffold surfaces upon vigorous washing or extended storage. Recombinant human bone morphogenetic protein 2 (rhBMP-2) was bound to conjugated scaffolds by ionic interactions between the negatively charged SO42- clusters of heparin and positively charged amino acids of rhBMP-2. The resulting scaffolds were inspected for bone regenerative capacity by subcutaneous implanting in rats. Histological observation and mineralization assay were performed after 4 weeks of implantation. Results from both in vitro and in vivo experiments suggest the potential of the developed scaffolds for bone tissue engineering applications in the future.
[Mh] Termos MeSH primário: Anticoagulantes/administração & dosagem
Proteína Morfogenética Óssea 2/administração & dosagem
Regeneração Óssea/efeitos dos fármacos
Materiais Revestidos Biocompatíveis/química
Sistemas de Liberação de Medicamentos
Heparina/administração & dosagem
Tecidos Suporte/química
[Mh] Termos MeSH secundário: Animais
Anticoagulantes/efeitos adversos
Anticoagulantes/química
Anticoagulantes/farmacologia
Proteína Morfogenética Óssea 2/química
Proteína Morfogenética Óssea 2/genética
Proteína Morfogenética Óssea 2/farmacologia
Fosfatos de Cálcio/efeitos adversos
Fosfatos de Cálcio/química
Quitosana/efeitos adversos
Quitosana/química
Quitosana/metabolismo
Materiais Revestidos Biocompatíveis/efeitos adversos
Reagentes para Ligações Cruzadas/química
Sistemas de Liberação de Medicamentos/efeitos adversos
Liberação Controlada de Fármacos
Etildimetilaminopropil Carbodi-Imida/química
Heparina/efeitos adversos
Heparina/química
Seres Humanos
Masculino
Polifosfatos/efeitos adversos
Polifosfatos/química
Polifosfatos/metabolismo
Ratos Wistar
Proteínas Recombinantes/administração & dosagem
Proteínas Recombinantes/efeitos adversos
Proteínas Recombinantes/química
Proteínas Recombinantes/farmacologia
Silicones/efeitos adversos
Silicones/química
Solubilidade
Propriedades de Superfície
Tecidos Suporte/efeitos adversos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anticoagulants); 0 (BMP2 protein, human); 0 (Bone Morphogenetic Protein 2); 0 (Calcium Phosphates); 0 (Coated Materials, Biocompatible); 0 (Cross-Linking Reagents); 0 (Polyphosphates); 0 (Recombinant Proteins); 0 (Silicones); 9005-49-6 (Heparin); 9012-76-4 (Chitosan); 97Z1WI3NDX (calcium phosphate); RJ5OZG6I4A (Ethyldimethylaminopropyl Carbodiimide)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160708
[St] Status:MEDLINE


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[PMID]:27325579
[Au] Autor:Ghodbane SA; Dunn MG
[Ad] Endereço:Orthopaedic Research Laboratories, Department of Orthopaedic Surgery, Rutgers Biomedical and Health Sciences- Robert Wood Johnson Medical School, Rutgers University, the State University of New Jersey. dunnmg@rwjms.rutgers.edu.
[Ti] Título:Physical and mechanical properties of cross-linked type I collagen scaffolds derived from bovine, porcine, and ovine tendons.
[So] Source:J Biomed Mater Res A;104(11):2685-92, 2016 Nov.
[Is] ISSN:1552-4965
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Collagen scaffolds are often utilized in tissue engineering applications where their performance depends on physical and mechanical properties. This study investigated the effects of collagen source (bovine, porcine, and ovine tendon) on properties of collagen sponge scaffolds cross-linked with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS). Scaffolds were tested for tensile and compressive properties, stability (resistance to enzymatic degradation), pore size, and swelling ratio. No significant differences in tensile modulus were observed, but ovine scaffolds had significantly greater ultimate strain, stress, and toughness relative to bovine and porcine scaffolds. No significant differences in compressive properties, pore size, or swelling ratio were observed as a function of collagen source. Ovine scaffolds were more resistant to collagenase degradation compared to bovine samples, which were more resistant than porcine scaffolds. In comparison to bovine scaffolds, ovine scaffolds performed equivalently or superiorly in all evaluations, and porcine scaffolds were equivalent in all properties except enzymatic stability. These results suggest that collagen sponges derived from bovine, porcine, and ovine tendon have similar physical and mechanical properties, and are all potentially suitable materials for various tissue engineering applications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2685-2692, 2016.
[Mh] Termos MeSH primário: Materiais Biocompatíveis/química
Colágeno Tipo I/química
Reagentes para Ligações Cruzadas/química
Tecidos Suporte/química
[Mh] Termos MeSH secundário: Animais
Bovinos
Etildimetilaminopropil Carbodi-Imida/química
Teste de Materiais
Porosidade
Proteólise
Ovinos
Especificidade da Espécie
Succinimidas/química
Suínos
Tendões/química
Resistência à Tração
Engenharia Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Collagen Type I); 0 (Cross-Linking Reagents); 0 (Succinimides); MJE3791M4T (N-hydroxysuccinimide); RJ5OZG6I4A (Ethyldimethylaminopropyl Carbodiimide)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160622
[St] Status:MEDLINE
[do] DOI:10.1002/jbm.a.35813


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[PMID]:27063248
[Au] Autor:Coughlin J; Masci A; Gronke RS; Bergelson S; Co C
[Ad] Endereço:Analytical Development, Biogen, Cambridge, MA, 02142, USA; Process Biochemistry, Biogen, Cambridge, MA, 02142, USA.
[Ti] Título:A simple enzyme-substrate localized conjugation method to generate immobilized, functional glutathione S-transferase fusion protein columns for affinity enrichment.
[So] Source:Anal Biochem;505:51-8, 2016 Jul 15.
[Is] ISSN:1096-0309
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Immobilized protein receptors and enzymes are tools for isolating or enriching ligands and substrates based on affinity. For example, glutathione S-transferase (GST) is fused to proteins as a tag for binding to its substrate glutathione (GSH) linked to solid supports. One issue with this approach is that high-affinity interactions between receptors and ligands require harsh elution conditions such as low pH, which can result in leached receptor. Another issue is the inherent nonspecific chemical conjugation of reactive groups such as N-hydroxysuccinimide (NHS) that couple lysines to solid supports; the nonspecificity of NHS may result in residue modifications near the binding site(s) of the receptor that can affect ligand specificity. In this study, a simple conjugation procedure is presented that overcomes these limitations and results in immobilized GST fusion proteins that are functional and specific. Here, the affinity of GST for GSH was used to generate an enzyme-substrate site-specific cross-linking reaction; GSH-Sepharose was preactivated with 1-ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and then incubated Fc gamma receptor IIIa (FcγRIIIa)-GST. The immobilized FcγRIIIa-GST more specifically bound glycosylated immunoglobulin G1s (IgG1s) and was used to enrich nonfucosylated IgG1s from weaker binding species. This technique can be used when modifications of amino acids lead to changes in activity.
[Mh] Termos MeSH primário: Enzimas Imobilizadas/química
Glutationa Transferase/química
Receptores de IgG/química
Proteínas Recombinantes de Fusão/química
[Mh] Termos MeSH secundário: Enzimas Imobilizadas/metabolismo
Etildimetilaminopropil Carbodi-Imida/química
Glutationa/química
Glutationa Transferase/metabolismo
Seres Humanos
Imunoglobulina G/química
Imunoglobulina G/metabolismo
Receptores de IgG/metabolismo
Proteínas Recombinantes de Fusão/metabolismo
Sefarose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzymes, Immobilized); 0 (FCGR3A protein, human); 0 (Immunoglobulin G); 0 (Receptors, IgG); 0 (Recombinant Fusion Proteins); 9012-36-6 (Sepharose); EC 2.5.1.18 (Glutathione Transferase); GAN16C9B8O (Glutathione); RJ5OZG6I4A (Ethyldimethylaminopropyl Carbodiimide)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160412
[St] Status:MEDLINE


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[PMID]:26972378
[Au] Autor:Menzel C; Silbernagl J; Laffleur F; Leichner C; Jelkmann M; Huck CW; Hussain S; Bernkop-Schnürch A
[Ad] Endereço:Center for Chemistry and Biomedicine, Department of Pharmaceutical Technology, Institute of Pharmacy, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria.
[Ti] Título:2,2'Dithiodinicotinyl ligands: Key to more reactive thiomers.
[So] Source:Int J Pharm;503(1-2):199-206, 2016 Apr 30.
[Is] ISSN:1873-3476
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to establish a novel type of preactivated thiomers exhibiting a comparatively higher reactivity with mucus and consequently improved mucoadhesive properties. In order to achieve this goal, the dimeric form of 2-mercaptonicotinic acid (MNA-MNA) was directly attached to the polymeric backbone of chitosan (CHI) via amide bond formation mediated by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDAC) used as a coupling reagent. The remaining free amino groups were in the following reacted with succinic anhydride (Succ) in order to obtain a uniformly anionically charged polymer (CHI-Succ-MNA-MNA). Within this study, different coupling rates of up to 170 µmol MNA-MNA per gram polymer were achieved. The attachment of the dimeric ligand resulted in a preactivated thiomer with a comparatively more reactive disulfide substructure due to the additional nitrogen atom in conjugation over the aromatic moieties. Furthermore, the obtained polymer is entirely preactivated and thus prevented against undesired oxidation reactions. Kinetic studies of disulfide exchange reactions showed a 3.8-fold higher reactivity of CHI-Succ-MNA-MNA in comparison to a state-of-the-art preactivated thiomer. Within rheological measurements, CHI-Succ-MNA-MNA with a coupling rate of 170 µmol (CHI-Succ-MNA-MNA 170) led to a 5.7-fold higher mucus viscosity than the non-thiolated control polymer (CHI-Succ) indicating a rheological synergism due to mucoadhesive properties. These results were confirmed by a second mucoadhesion study, which showed a significantly prolonged retention time of CHI-Succ-MNA-MNA on the small intestinal mucosa compared to CHI-Succ (P<0.02). Accordingly, the double preactivation seems to be a promising strategy in order to obtain entirely preactivated polymers with enhanced mucoadhesive properties.
[Mh] Termos MeSH primário: Quitosana/química
Etildimetilaminopropil Carbodi-Imida/química
Ácidos Nicotínicos/química
Polímeros/química
Anidridos Succínicos/química
Compostos de Sulfidrila/química
[Mh] Termos MeSH secundário: Adesividade
Animais
Células CACO-2
Sobrevivência Celular/efeitos dos fármacos
Seres Humanos
Mucosa Intestinal/química
Ligantes
Polímeros/farmacologia
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ligands); 0 (Nicotinic Acids); 0 (Polymers); 0 (Succinic Anhydrides); 0 (Sulfhydryl Compounds); 6RF4O17Z8J (succinic anhydride); 9012-76-4 (Chitosan); EU7D859ABZ (2-mercaptonicotinic acid); RJ5OZG6I4A (Ethyldimethylaminopropyl Carbodiimide)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170104
[Lr] Data última revisão:
170104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160315
[St] Status:MEDLINE


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[PMID]:26928466
[Au] Autor:Choi DH; Kang SN; Kim SM; Gobaa S; Park BJ; Kim IH; Joung YK; Han DK
[Ad] Endereço:Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 136-791, Republic of Korea; School of life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
[Ti] Título:Growth factors-loaded stents modified with hyaluronic acid and heparin for induction of rapid and tight re-endothelialization.
[So] Source:Colloids Surf B Biointerfaces;141:602-610, 2016 May 01.
[Is] ISSN:1873-4367
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Rapid re-endothelialization of damaged vessel lining efficiently prevents restenosis and thrombosis and restores original vascular functions. In this study, we designed a novel metallic stent with a heparin-modified surface and used different methods, including 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and divinyl sulfone (DVS), to load growth factors. First we loaded heparin into a dopamine-conjugated hyaluronic acid (HA) coating to serve as a growth factor reservoir. In a second step, we took advantage of the heparin-binding domain of vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) to gain advanced re-endothelialization capabilities. We demonstrated that DVS technique offered higher amount of growth factor loading. In vitro assessment also showed better capillary-like structure formation and localized gap junctions when DVS coating was employed. This study suggested that growth factor loaded stent modified by HA and heparin provided the advantage to rapid and tight restoration of endothelium.
[Mh] Termos MeSH primário: Stents Farmacológicos
Heparina/química
Fator de Crescimento de Hepatócito/farmacologia
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Ácido Hialurônico/química
Fator A de Crescimento do Endotélio Vascular/farmacologia
[Mh] Termos MeSH secundário: Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Liberação Controlada de Fármacos
Etildimetilaminopropil Carbodi-Imida/química
Fator de Crescimento de Hepatócito/química
Fator de Crescimento de Hepatócito/farmacocinética
Células Endoteliais da Veia Umbilical Humana/fisiologia
Seres Humanos
Espectroscopia de Ressonância Magnética
Microscopia Eletrônica de Varredura
Microscopia de Fluorescência
Espectroscopia de Infravermelho com Transformada de Fourier/métodos
Sulfonas/química
Propriedades de Superfície
Fator A de Crescimento do Endotélio Vascular/química
Fator A de Crescimento do Endotélio Vascular/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Sulfones); 0 (Vascular Endothelial Growth Factor A); 5PFN71LP8M (divinyl sulfone); 67256-21-7 (Hepatocyte Growth Factor); 9004-61-9 (Hyaluronic Acid); 9005-49-6 (Heparin); RJ5OZG6I4A (Ethyldimethylaminopropyl Carbodiimide)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE


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[PMID]:26871636
[Au] Autor:Sylwestrak EL; Rajasethupathy P; Wright MA; Jaffe A; Deisseroth K
[Ad] Endereço:Department of Bioengineering, Stanford University, 318 Campus Drive, Stanford, CA 94305, USA.
[Ti] Título:Multiplexed Intact-Tissue Transcriptional Analysis at Cellular Resolution.
[So] Source:Cell;164(4):792-804, 2016 Feb 11.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In recently developed approaches for high-resolution imaging within intact tissue, molecular characterization over large volumes has been largely restricted to labeling of proteins. But volumetric nucleic acid labeling may represent a far greater scientific and clinical opportunity, enabling detection of not only diverse coding RNA variants but also non-coding RNAs. Moreover, scaling immunohistochemical detection to large tissue volumes has limitations due to high cost, limited renewability/availability, and restricted multiplexing capability of antibody labels. With the goal of versatile, high-content, and scalable molecular phenotyping of intact tissues, we developed a method using carbodiimide-based chemistry to stably retain RNAs in clarified tissue, coupled with amplification tools for multiplexed detection. The resulting technology enables robust measurement of activity-dependent transcriptional signatures, cell-identity markers, and diverse non-coding RNAs in rodent and human tissue volumes. The growing set of validated probes is deposited in an online resource for nucleating related developments from across the scientific community.
[Mh] Termos MeSH primário: Química Encefálica
Hibridização In Situ/métodos
Técnicas de Amplificação de Ácido Nucleico/métodos
RNA/análise
Transcriptoma
[Mh] Termos MeSH secundário: Adolescente
Animais
Cianatos/química
Etildimetilaminopropil Carbodi-Imida/química
Feminino
Seres Humanos
Masculino
Maleimidas/química
Camundongos
Meia-Idade
Oligonucleotídeos/química
Succinimidas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Cyanates); 0 (Maleimides); 0 (Oligonucleotides); 0 (Succinimides); 123457-83-0 (4-maleimidophenyl isocyanate); 63231-63-0 (RNA); RJ5OZG6I4A (Ethyldimethylaminopropyl Carbodiimide); V9WYZ7QMDT (disuccinimidyl suberate)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160213
[St] Status:MEDLINE


  9 / 607 MEDLINE  
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[PMID]:26854392
[Au] Autor:Malcor JD; Bax D; Hamaia SW; Davidenko N; Best SM; Cameron RE; Farndale RW; Bihan D
[Ad] Endereço:Department of Biochemistry, University of Cambridge, Downing Site, Cambridge, CB2 1QW, UK.
[Ti] Título:The synthesis and coupling of photoreactive collagen-based peptides to restore integrin reactivity to an inert substrate, chemically-crosslinked collagen.
[So] Source:Biomaterials;85:65-77, 2016 Apr.
[Is] ISSN:1878-5905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Collagen is frequently advocated as a scaffold for use in regenerative medicine. Increasing the mechanical stability of a collagen scaffold is widely achieved by cross-linking using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). However, this treatment consumes the carboxylate-containing amino acid sidechains that are crucial for recognition by the cell-surface integrins, abolishing cell adhesion. Here, we restore cell reactivity to a cross-linked type I collagen film by covalently linking synthetic triple-helical peptides (THPs), mimicking the structure of collagen. These THPs are ligands containing an active cell-recognition motif, GFOGER, a high-affinity binding site for the collagen-binding integrins. We end-stapled peptide strands containing GFOGER by coupling a short diglutamate-containing peptide to their N-terminus, improving the thermal stability of the resulting THP. A photoreactive Diazirine group was grafted onto the end-stapled THP to allow covalent linkage to the collagen film upon UV activation. Such GFOGER-derivatized collagen films showed restored affinity for the ligand-binding I domain of integrin α2ß1, and increased integrin-dependent cell attachment and spreading of HT1080 and Rugli cell lines, expressing integrins α2ß1 and α1ß1, respectively. The method we describe has wide application, beyond collagen films or scaffolds, since the photoreactive diazirine will react with many organic carbon skeletons.
[Mh] Termos MeSH primário: Colágeno Tipo I/química
Integrina alfa1beta1/química
Integrina alfa2beta1/química
Peptídeos/química
[Mh] Termos MeSH secundário: Sítios de Ligação
Adesão Celular
Linhagem Celular Tumoral
Diazometano/farmacologia
Etildimetilaminopropil Carbodi-Imida/química
Seres Humanos
Ligação Proteica
Succinimidas/química
Tecidos Suporte/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Collagen Type I); 0 (Integrin alpha1beta1); 0 (Integrin alpha2beta1); 0 (Peptides); 0 (Succinimides); 60A625P70P (Diazomethane); MJE3791M4T (N-hydroxysuccinimide); RJ5OZG6I4A (Ethyldimethylaminopropyl Carbodiimide)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160209
[St] Status:MEDLINE


  10 / 607 MEDLINE  
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[PMID]:26838920
[Au] Autor:Hua J; Li Z; Xia W; Yang N; Gong J; Zhang J; Qiao C
[Ad] Endereço:Key Laboratory of Advanced Textile Composites, Tianjin Polytechnic University, Ministry of Education, Tianjin 300387, China; School of Textiles, Tianjin Polytechnic University, Tianjin 300387, China.
[Ti] Título:Preparation and properties of EDC/NHS mediated crosslinking poly (gamma-glutamic acid)/epsilon-polylysine hydrogels.
[So] Source:Mater Sci Eng C Mater Biol Appl;61:879-92, 2016 Apr 01.
[Is] ISSN:1873-0191
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this paper, a novel pH-sensitive poly (amino acid) hydrogel based on poly γ-glutamic acid (γ-PGA) and ε-polylysine (ε-PL) was prepared by carbodiimide (EDC) and N-hydroxysuccinimide (NHS) mediated polymerization. The influence of PGA/PL molar ratio and EDC/NHS concentration on the structure and properties was studied. Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) proved that hydrogels were crosslinked through amide bond linkage, and the conversion rate of a carboxyl group could reach 96%. Scanning electron microscopy (SEM) results showed a regularly porous structure with 20 µm pore size in average. The gelation time in the crosslink process of PGA/PL hydrogels was within less than 5 min. PGA/PL hydrogels had excellent optical performance that was evaluated by a novel optotype method. Furthermore, PGA/PL hydrogels were found to be pH-sensitive, which could be adjusted to the pH of swelling media intelligently. The terminal pH of swelling medium could be controlled at 5 ± 1 after equilibrium when the initial pH was within 3-11. The swelling kinetics was found to follow a Voigt model in deionized water but a pseudo-second-order model in normal saline and phosphate buffer solution, respectively. The differential swelling degrees were attributed to the swelling theory based on the different ratio of -COOH/-NH2 and pore size in hydrogels. The results of mechanical property indicated that PGA/PL hydrogels were soft and elastic. Moreover, PGA/PL hydrogels exhibited excellent biocompatibility by cell proliferation experiment. PGA/PL hydrogels could be degraded in PBS solution and the degradation rate was decreased with the increase of the molar ratio of PL. Considering the simple preparation process and pH-sensitive property, these PGA/PL hydrogels might have high potential for use in medical and clinical fields.
[Mh] Termos MeSH primário: Hidrogéis/química
Ácido Poliglutâmico/análogos & derivados
Polilisina/química
[Mh] Termos MeSH secundário: Animais
Materiais Biocompatíveis/química
Materiais Biocompatíveis/farmacologia
Adesão Celular/efeitos dos fármacos
Linhagem Celular
Proliferação Celular/efeitos dos fármacos
Etildimetilaminopropil Carbodi-Imida/química
Concentração de Íons de Hidrogênio
Camundongos
Microscopia Eletrônica de Varredura
Espectroscopia Fotoeletrônica
Ácido Poliglutâmico/química
Porosidade
Espectroscopia de Infravermelho com Transformada de Fourier
Succinimidas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biocompatible Materials); 0 (Hydrogels); 0 (Succinimides); 0 (poly(gamma-glutamic acid)); 25104-18-1 (Polylysine); 25513-46-6 (Polyglutamic Acid); MJE3791M4T (N-hydroxysuccinimide); RJ5OZG6I4A (Ethyldimethylaminopropyl Carbodiimide)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160204
[St] Status:MEDLINE



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