Base de dados : MEDLINE
Pesquisa : D02.522.296.100 [Categoria DeCS]
Referências encontradas : 363 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 37 ir para página                         

  1 / 363 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28873628
[Au] Autor:Xiao Z; Zhao L; Tian L; Wang L; Zhao JY
[Ad] Endereço:Center for Bioengineering and Biotechnology, State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), Qingdao 266580, China. Electronic address: zjxiao@upc.edu.cn.
[Ti] Título:GC-FID determination of tetramethylpyrazine and acetoin in vinegars and quantifying the dependence of tetramethylpyrazine on acetoin and ammonium.
[So] Source:Food Chem;239:726-732, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:2,3,5,6-Tetramethylpyrazine (TMP) is an important health functional composition in vinegars, but there are controversial viewpoints about its formation mechanism and scarce relevant records on TMP content enhancement in vinegar products. In this study, a simple and accurate solvent extraction coupled with GC-FID method was developed for the simultaneous determination of TMP and acetoin in vinegars and 137 worldwide samples were analyzed. Meanwhile, the ammonium contents of all the vinegar samples were determined using the salicylate method. By nonlinear surface fitting, the concentrations of TMP in vinegars were found to follow second-order polynomial models of acetoin and ammonium concentrations. By isotopic tracing using C labelled acetoin and N ammonium, acetoin and ammonium were deduced to be the precursors of TMP in vinegars.
[Mh] Termos MeSH primário: Ácido Acético/química
[Mh] Termos MeSH secundário: Acetoína
Compostos de Amônio
Pirazinas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ammonium Compounds); 0 (Pyrazines); BG4D34CO2H (Acetoin); Q40Q9N063P (Acetic Acid); V80F4IA5XG (tetramethylpyrazine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  2 / 363 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28875495
[Au] Autor:Pu Z; Ji F; Wang J; Zhang Y; Sun W; Bao Y
[Ad] Endereço:School of Life Science and Biotechnology, Dalian University of Technology, China.
[Ti] Título:Rational design of Meso-2,3-butanediol dehydrogenase by molecular dynamics simulation and experimental evaluations.
[So] Source:FEBS Lett;591(20):3402-3413, 2017 Oct.
[Is] ISSN:1873-3468
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Meso-2,3-butanediol dehydrogenase (meso-2,3-BDH) catalyzes NAD -dependent conversion of meso-2,3-butanediol to acetoin, a crucial external energy storage molecule in fermentive bacteria. In this study, the active tunnel of meso-2,3-BDH was identified. The two short α helixes positioned away from the α4-helix possibly expose the hydrophobic ligand-binding cavity, gating the exit of product and cofactor from the activity pocket. Further MM/GBSA-binding free energy analysis shows that Phe212 and Asn146 function as the key product-release sites. Site-directed mutagenesis experiments targeted to the sites show that the k of Phe212Tyr is enhanced up to (4-8)-fold. The original activity of Asn146Gln is retained, but the activity of Asn146Ala mutation is lost. These results could provide helpful guidance on rational design of short-chain dehydrogenases/reductases.
[Mh] Termos MeSH primário: Oxirredutases do Álcool/química
Proteínas de Bactérias/química
Klebsiella pneumoniae/genética
Simulação de Acoplamento Molecular
Simulação de Dinâmica Molecular
[Mh] Termos MeSH secundário: Acetoína/química
Acetoína/metabolismo
Oxirredutases do Álcool/genética
Oxirredutases do Álcool/metabolismo
Sequência de Aminoácidos
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Sítios de Ligação
Butileno Glicóis/química
Butileno Glicóis/metabolismo
Clonagem Molecular
Cristalografia por Raios X
Escherichia coli/enzimologia
Escherichia coli/genética
Expressão Gênica
Cinética
Klebsiella pneumoniae/enzimologia
Modelos Moleculares
Mutagênese Sítio-Dirigida
Ligação Proteica
Conformação Proteica em alfa-Hélice
Engenharia de Proteínas
Domínios e Motivos de Interação entre Proteínas
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Alinhamento de Sequência
Homologia de Sequência de Aminoácidos
Especificidade por Substrato
Termodinâmica
[Pt] Tipo de publicação:LETTER
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Butylene Glycols); 0 (Recombinant Proteins); 45427ZB5IJ (2,3-butylene glycol); BG4D34CO2H (Acetoin); EC 1.1.- (Alcohol Oxidoreductases); EC 1.1.1.4 (butanediol dehydrogenase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1002/1873-3468.12834


  3 / 363 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28602402
[Au] Autor:Lazazzara V; Perazzolli M; Pertot I; Biasioli F; Puopolo G; Cappellin L
[Ad] Endereço:Department of Sustainable Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach, Via Mach 1, 38010 San Michele all'Adige, Italy; Centre for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU
[Ti] Título:Growth media affect the volatilome and antimicrobial activity against Phytophthora infestans in four Lysobacter type strains.
[So] Source:Microbiol Res;201:52-62, 2017 Aug.
[Is] ISSN:1618-0623
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Bacterial volatile organic compounds (VOCs) play important ecological roles in soil microbial interactions. Lysobacter spp. are key determinants of soil suppressiveness against phytopathogens and the production of non-volatile antimicrobial metabolites has been extensively characterised. However, the chemical composition and antagonistic properties of the Lysobacter volatilome have been poorly investigated. In this work, VOC emission profiles of four Lysobacter type strains grown on a sugar-rich and a protein-rich medium were analysed using solid-phase microextraction gas chromatography-mass spectrometry and proton transfer reaction-time of flight-mass spectrometry. Lysobacter antibioticus, L. capsici, L. enzymogenes and L. gummosus type strains were recognised according to their volatilome assessed using both headspace mass spectrometry methods Moreover, the chemical profiles and functional properties of the Lysobacter volatilome differed according to the growth medium, and a protein-rich substrate maximised the toxic effect of the four Lysobacter type strains against Phytophthora infestans. Antagonistic (pyrazines, pyrrole and decanal) and non-antagonistic (delta-hexalactone and ethanol) VOCs against Ph. infestans or putative plant growth stimulator compounds (acetoin and indole) were mainly emitted by Lysobacter type strains grown on protein- and sugar-rich media respectively. Thus nutrient availability under soil conditions could affect the aggressiveness of Lysobacter spp. and possibly optimise interactions of these bacterial species with the other soil inhabitants.
[Mh] Termos MeSH primário: Anti-Infecciosos/metabolismo
Meios de Cultura/química
Lysobacter/crescimento & desenvolvimento
Lysobacter/metabolismo
Phytophthora infestans/efeitos dos fármacos
Compostos Orgânicos Voláteis/antagonistas & inibidores
Compostos Orgânicos Voláteis/química
[Mh] Termos MeSH secundário: Acetoína/metabolismo
Aldeídos/antagonistas & inibidores
Agentes de Controle Biológico/antagonistas & inibidores
Metabolismo dos Carboidratos
Etanol/farmacologia
Cromatografia Gasosa-Espectrometria de Massas/métodos
Indóis/metabolismo
Lysobacter/classificação
Espectrometria de Massas
Interações Microbianas
Phytophthora infestans/crescimento & desenvolvimento
Proteínas/metabolismo
Pirazinas/antagonistas & inibidores
Pirróis/antagonistas & inibidores
Solo
Microbiologia do Solo
Compostos Orgânicos Voláteis/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aldehydes); 0 (Anti-Infective Agents); 0 (Biological Control Agents); 0 (Culture Media); 0 (Indoles); 0 (Proteins); 0 (Pyrazines); 0 (Pyrroles); 0 (Soil); 0 (Volatile Organic Compounds); 31Z90Q7KQJ (decanaldehyde); 3K9958V90M (Ethanol); 8724FJW4M5 (indole); BG4D34CO2H (Acetoin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE


  4 / 363 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28578227
[Au] Autor:Zhang L; Huang J; Zhou R; Wu C
[Ad] Endereço:College of Light Industry, Textile & Food Engineering, Sichuan University, Chengdu 610065, China; Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, Sichuan University, Chengdu 610065, China.
[Ti] Título:Evaluating the feasibility of fermentation starter inoculated with Bacillus amyloliquefaciens for improving acetoin and tetramethylpyrazine in Baoning bran vinegar.
[So] Source:Int J Food Microbiol;255:42-50, 2017 Aug 16.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Fermentation starters (Daqu) used in present study included traditional herb Daqu (C Daqu), modified Daqu without herbs (M Daqu) and S Daqu fermented by inoculating acetoin and tetramethylpyrazine high-producing bacterium Bacillus amyloliquefaciens into M Daqu. To evaluate the feasibility of S Daqu combined with M Daqu applied for improving contents of acetoin and tetramethylpyrazine in Baoning bran vinegar without remarkably changing the original microbial community and the other volatiles contents compared with C Daqu, vinegar Pei C, M, M1, M2 and S were correspondingly prepared in lab scale using C Daqu, M Daqu, M1 Daqu (S Daqu: M Daqu=1:9, w/w), M2 Daqu (S Daqu: M Daqu=5:5) and S Daqu. PCR-DGGE suggested that Bacillus, Lactobacillus, Oceanobacillus, Acetobacter, Pichia, Geotrichum and Trichoderma were dominant microbes. Microbial community of M were similar with M1, while that of the others were similar. Differences in physicochemical properties among samples may be ascribed to different enzymes activities of Daqu and bioactivities of microbial metabolism during fermentation. Moreover, total contents of organic acids in M, M1, M2 and S increased by 33.10%, 25.77%, 4.32% and 7.74% relative to C, respectively. Volatiles and PLS-DA analysis suggested that volatile profiles of M were similar with M1, that of M2 were similar with C, while that of S were significantly different with the others. Both M2 Daqu and S Daqu facilitated the formation of acetoin and tetramethylpyrazine. However, M2 Daqu was more efficient for enhancing acetoin and tetramethylpyrazine contents by 191.84% and 123.17% respectively, without significantly changing the other volatiles contents.
[Mh] Termos MeSH primário: Ácido Acético/química
Ácido Acético/metabolismo
Acetoína/metabolismo
Bacillus amyloliquefaciens/metabolismo
Fermentação
Microbiologia de Alimentos
Pirazinas/metabolismo
[Mh] Termos MeSH secundário: Acetobacter/genética
Acetobacter/metabolismo
Acetoína/análise
Bacillus/genética
Bacillus/metabolismo
Bactérias/genética
Bactérias/metabolismo
Fungos/genética
Fungos/metabolismo
Reação em Cadeia da Polimerase
Pirazinas/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pyrazines); BG4D34CO2H (Acetoin); Q40Q9N063P (Acetic Acid); V80F4IA5XG (tetramethylpyrazine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170605
[St] Status:MEDLINE


  5 / 363 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28558330
[Au] Autor:Peralta GH; Bergamini CV; Audero G; Páez R; Wolf IV; Perotti MC; Hynes ER
[Ad] Endereço:Instituto de Lactología Industrial, Universidad Nacional del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas, Santiago del Estero 2829, S3000AOM Santa Fe, Argentina. Electronic address: gperalta@fiq.unl.edu.ar.
[Ti] Título:Spray-dried adjunct cultures of autochthonous non-starter lactic acid bacteria.
[So] Source:Int J Food Microbiol;255:17-24, 2017 Aug 16.
[Is] ISSN:1879-3460
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Spray-drying of lactic cultures provides direct-to-vat starters, which facilitate their commercialization and use. However, this process may alter the metabolic activity and deteriorate technological features. In this work, we assessed the influence of spray-drying on the survival and aroma production of two strains of mesophilic lactobacilli: Lactobacillus paracasei 90 and Lactobacillus plantarum 91, which have already been characterized as good adjunct cultures. The spray-drying was carried out using a laboratory scale spray and the dried cultures were monitored during the storage for the survival rate. The dried cultures were applied to two cheese models: sterile cheese extract and miniature soft cheese. The influence on the carbohydrate metabolism and the production of organic acids and volatile compounds was determined. Both strains retained high levels of viable counts in the powder after drying and during the storage at 5°C for twelve months. In addition, they also remained at high level in both cheese models during incubation or ripening. Similar profiles of carbohydrate fermentation and bioformation of volatile compounds were observed in the cheese extracts for each of the strains when tested as both fresh and dried cultures. In addition, the ability of Lb. paracasei 90 to increase the production of acetoin and diacetyl remarkably in cheese models was also confirmed for the spray-dried culture.
[Mh] Termos MeSH primário: Queijo/microbiologia
Dessecação
Manipulação de Alimentos/métodos
Manipulação de Alimentos/normas
Microbiologia de Alimentos
Lactobacillus plantarum/fisiologia
Lactobacillus/fisiologia
[Mh] Termos MeSH secundário: Acetoína/metabolismo
Metabolismo dos Carboidratos
Queijo/normas
Diacetil/metabolismo
Fermentação
Lactobacillus/metabolismo
Lactobacillus plantarum/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
BG4D34CO2H (Acetoin); K324J5K4HM (Diacetyl)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170531
[St] Status:MEDLINE


  6 / 363 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28531323
[Au] Autor:Cha DH; Landolt PJ; Adams TB
[Ad] Endereço:USDA-ARS, US Pacific Basin Agricultural Research Center, 64 Nowelo St., Hilo, HA 96720.
[Ti] Título:Effect of Chemical Ratios of a Microbial-Based Feeding Attractant on Trap Catch of Drosophila suzukii (Diptera: Drosophilidae).
[So] Source:Environ Entomol;46(4):907-915, 2017 08 01.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Drosophila suzukii Matsumura, spotted wing drosophila, can be trapped with a feeding attractant based on wine and vinegar volatiles and consisting of acetic acid, ethanol, acetoin, and methionol. Using that four-component blend, we found that the catch of spotted wing drosophila increased with increases in the release rate of acetoin (from 0.5 mg/d to 34 mg/d) from polyethylene sachet dispensers, and with increases in the concentrations of acetic acid (from 0.25% to 4%) or ethanol (from 0.08% to 2%) when dispensed in the trap drowning solution. However, we saw no increase in spotted wing drosophila trapped with increase of the methionol release rate from 0.4 mg/d to 4.9 mg/d or from 0.19 mg/d to 0.8 mg/d, from sachets. A new formulation based on optimized amounts of these four chemicals yielded a doubling of spotted wing drosophila trapped compared to a previously reported formulation. Further field testing confirmed that the simultaneous increases in the release rate of acetoin from a dispenser and the amount of acetic acid in the trap drowning solution provided the increased spotted wing drosophila trap response to the new formulation. These findings provide a practical means to improve the power of this lure to detect and monitor D. suzukii.
[Mh] Termos MeSH primário: Quimiotaxia
Drosophila/efeitos dos fármacos
Controle de Insetos
Feromônios/farmacologia
[Mh] Termos MeSH secundário: Ácido Acético/farmacologia
Acetoína/farmacologia
Animais
Relação Dose-Resposta a Droga
Drosophila/fisiologia
Etanol/farmacologia
Feminino
Masculino
Propanóis/farmacologia
Sulfetos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Pheromones); 0 (Propanols); 0 (Sulfides); 0 (insect attractants); 3K9958V90M (Ethanol); BG4D34CO2H (Acetoin); H1E1U441XX (methionol); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvx079


  7 / 363 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28423947
[Au] Autor:Yang T; Rao Z; Zhang X; Xu M; Xu Z; Yang ST
[Ad] Endereço:a The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology , Jiangnan University , Wuxi , China.
[Ti] Título:Metabolic engineering strategies for acetoin and 2,3-butanediol production: advances and prospects.
[So] Source:Crit Rev Biotechnol;37(8):990-1005, 2017 Dec.
[Is] ISSN:1549-7801
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Acetoin and 2,3-butanediol (2,3-BD) have a large number of industrial applications. The production of acetoin and 2,3-BD has traditionally relied on oil supplies. Microbial production of acetoin and 2,3-BD will alleviate the dependence on oil. Acetoin and 2,3-BD are neighboring metabolites in the 2,3-BD metabolic pathway of bacteria. This review summarizes metabolic engineering strategies for improvement of microbial acetoin and 2,3-BD production. We also propose enhancements to current acetoin and 2,3-BD production strategies, by offering a metabolic engineering approach that is guided by systems biology and synthetic biology.
[Mh] Termos MeSH primário: Engenharia Metabólica
[Mh] Termos MeSH secundário: Acetoína
Butileno Glicóis
Fermentação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Butylene Glycols); 45427ZB5IJ (2,3-butylene glycol); BG4D34CO2H (Acetoin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1080/07388551.2017.1299680


  8 / 363 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28187139
[Au] Autor:Khan AR; Park GS; Asaf S; Hong SJ; Jung BK; Shin JH
[Ad] Endereço:School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea.
[Ti] Título:Complete genome analysis of Serratia marcescens RSC-14: A plant growth-promoting bacterium that alleviates cadmium stress in host plants.
[So] Source:PLoS One;12(2):e0171534, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Serratia marcescens RSC-14 is a Gram-negative bacterium that was previously isolated from the surface-sterilized roots of the Cd-hyperaccumulator Solanum nigrum. The strain stimulates plant growth and alleviates Cd stress in host plants. To investigate the genetic basis for these traits, the complete genome of RSC-14 was obtained by single-molecule real-time sequencing. The genome of S. marcescens RSC-14 comprised a 5.12-Mbp-long circular chromosome containing 4,593 predicted protein-coding genes, 22 rRNA genes, 88 tRNA genes, and 41 pseudogenes. It contained genes with potential functions in plant growth promotion, including genes involved in indole-3-acetic acid (IAA) biosynthesis, acetoin synthesis, and phosphate solubilization. Moreover, annotation using NCBI and Rapid Annotation using Subsystem Technology identified several genes that encode antioxidant enzymes as well as genes involved in antioxidant production, supporting the observed resistance towards heavy metals, such as Cd. The presence of IAA pathway-related genes and oxidative stress-responsive enzyme genes may explain the plant growth-promoting potential and Cd tolerance, respectively. This is the first report of a complete genome sequence of Cd-tolerant S. marcescens and its plant growth promotion pathway. The whole-genome analysis of this strain clarified the genetic basis underlying its phenotypic and biochemical characteristics, underpinning the beneficial interactions between RSC-14 and plants.
[Mh] Termos MeSH primário: Cádmio/toxicidade
Genes Bacterianos
Interações Hospedeiro-Patógeno/genética
Estresse Oxidativo
Serratia marcescens/genética
[Mh] Termos MeSH secundário: Acetoína/metabolismo
Ácidos Indolacéticos/metabolismo
Anotação de Sequência Molecular
Fosfatos/metabolismo
Solanum/efeitos dos fármacos
Solanum/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoleacetic Acids); 0 (Phosphates); 00BH33GNGH (Cadmium); 6U1S09C61L (indoleacetic acid); BG4D34CO2H (Acetoin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171534


  9 / 363 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28059435
[Au] Autor:Bursac T; Gralnick JA; Gescher J
[Ad] Endereço:Department of Applied Biology, Institute for Applied Biosciences, Karlsruhe Institute of Technology, Karlsruhe, Germany.
[Ti] Título:Acetoin production via unbalanced fermentation in Shewanella oneidensis.
[So] Source:Biotechnol Bioeng;114(6):1283-1289, 2017 Jun.
[Is] ISSN:1097-0290
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study describes the realization of an anoxic acetoin production process using the proteobacterium Shewanella oneidensis. Fermentative processes are of high biotechnological relevance since they offer high productivity and a low percentage of substrate consumption for anabolic processes. Nevertheless, the range of compounds that can be produced as sole end product of a fermentative process is limited, since the average oxidation state of substrate and products has to be identical in the absence of an external electron acceptor. This limitation could be overcome by the transfer of the surplus of electrons to a poised electrode surface, which of note is the only known anaerobic electron acceptor that cannot be depleted. In the first genetic engineering step, deletion mutants were developed that are devoid of either one, two, or all three prophages in their genome with the aim to construct a more stable chassis strain for microbe-electrode interaction, due to less prophage induced cell lysis (Gödeke et al., 2011). Current production in a bioelectrochemical system together with the analysis of cells on the anode surface were used as surrogate for the stability assessment. The λ-prophage deletion mutant produced overall 1.34fold more current (6.7 µA cm ) than the wild type and all other constructed strains and showed with 1.1 × 10 cells the highest cell density on the anode surface (2.3fold more than the wild type). The strain was further modified to contain codon optimized versions of acetolactate synthase and acetolactate decarboxylase derived from Bacillus subtilis. This allowed for the production of a mixture of acetoin and acetate from lactate in an almost 0.4:1 ratio. Further process improvement was reached by the deletion of the acetate kinase and phosphotransacetylase genes ackA/pta. The acetoin yield increased in this mutant from 40 to 86% of the theoretical maximum and acetoin was the only detectable end product. Biotechnol. Bioeng. 2017;114: 1283-1289. © 2017 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Acetoína/metabolismo
Acetolactato Sintase/genética
Reatores Biológicos/microbiologia
Carboxiliases/genética
Melhoramento Genético/métodos
Shewanella/fisiologia
[Mh] Termos MeSH secundário: Acetoína/isolamento & purificação
Acetolactato Sintase/metabolismo
Bacillus subtilis/enzimologia
Bacillus subtilis/genética
Carboxiliases/metabolismo
Fermentação
Proteínas Recombinantes/metabolismo
Shewanella/classificação
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Proteins); BG4D34CO2H (Acetoin); EC 2.2.1.6 (Acetolactate Synthase); EC 4.1.1.- (Carboxy-Lyases); EC 4.1.1.5 (acetolactate decarboxylase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170607
[Lr] Data última revisão:
170607
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.1002/bit.26243


  10 / 363 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27878591
[Au] Autor:Dai JY; Ma LH; Wang ZF; Guan WT; Xiu ZL
[Ad] Endereço:School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116024, People's Republic of China.
[Ti] Título:Sugaring-out extraction of acetoin from fermentation broth by coupling with fermentation.
[So] Source:Bioprocess Biosyst Eng;40(3):423-429, 2017 Mar.
[Is] ISSN:1615-7605
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Acetoin is a natural flavor and an important bio-based chemical which could be separated from fermentation broth by solvent extraction, salting-out extraction or recovered in the form of derivatives. In this work, a novel method named as sugaring-out extraction coupled with fermentation was tried in the acetoin production by Bacillus subtilis DL01. The effects of six solvents on bacterial growth and the distribution of acetoin and glucose in different solvent-glucose systems were explored. The operation parameters such as standing time, glucose concentration, and volume ratio of ethyl acetate to fermentation broth were determined. In a system composed of fermentation broth, glucose (100%, m/v) and two-fold volume of ethyl acetate, nearly 100% glucose was distributed into bottom phase, and 61.2% acetoin into top phase without coloring matters and organic acids. The top phase was treated by vacuum distillation to remove solvent and purify acetoin, while the bottom phase was used as carbon source to produce acetoin in the next batch of fermentation.
[Mh] Termos MeSH primário: Acetoína/química
Bacillus subtilis/metabolismo
Fermentação
Microbiologia Industrial/métodos
[Mh] Termos MeSH secundário: Acetatos/química
Ácidos/química
Bacillus subtilis/genética
Butileno Glicóis/química
Técnicas de Química Analítica
Cor
Meios de Cultura/química
Glucose/química
Engenharia Metabólica
Solventes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Acids); 0 (Butylene Glycols); 0 (Culture Media); 0 (Solvents); 76845O8NMZ (ethyl acetate); BG4D34CO2H (Acetoin); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161124
[St] Status:MEDLINE
[do] DOI:10.1007/s00449-016-1710-x



página 1 de 37 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde