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[PMID]: 27770422 [Au] Autor: Hickey DP [Ad] Endereço: Department of Chemistry, University of Utah, 315 S 1400 E, Salt Lake City, UT, 84112, USA. dhickeychem@gmail.com. [Ti] Título: Ferrocene-Modified Linear Poly(ethylenimine) for Enzymatic Immobilization and Electron Mediation. [So] Source: Methods Mol Biol;1504:181-191, 2017. [Is] ISSN: 1940-6029 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Enzymatic glucose biosensors and biofuel cells make use of the electrochemical transduction between an oxidoreductase enzyme, such as glucose oxidase (GOx), and an electrode to either quantify the amount of glucose in a solution or generate electrical energy. However, many enzymes including GOx are not able to electrochemically interact with an electrode surface directly, but require an external electrochemical relay to shuttle electrons to the electrode. Ferrocene-modified linear poly(ethylenimine) (Fc-LPEI) redox polymers have been designed to simultaneously immobilize glucose oxidase (GOx) at an electrode and mediate electron transfer from their flavin adenine dinucleotide (FAD) active site to the electrode surface. Cross-linked films of Fc-LPEI create hydrogel networks that allow for rapid transport of glucose, while the covalently bound ferrocene moieties are able to facilitate rapid electron transfer due to the ability of ferrocene to exchange electrons between adjacent ferrocene residues. For these reasons, Fc-LPEI films have been widely used in the development of high current density bioanode materials. This chapter describes the synthesis of a commonly used dimethylferrocene-modified linear poly(ethylenimine), as well as the subsequent preparation and electrochemical characterization of a GOx bioanode film utilizing the synthesized polymer. [Mh] Termos MeSH primário: Aspergillus niger/enzimologia Técnicas Biossensoriais/métodos Enzimas Imobilizadas/química Compostos Ferrosos/química Glucose Oxidase/química Glucose/análise Metalocenos/química Polietilenoimina/análogos & derivados [Mh] Termos MeSH secundário: Aspergillus niger/química Aspergillus niger/metabolismo Eletrodos Transporte de Elétrons Elétrons Enzimas Imobilizadas/metabolismo Glucose/metabolismo Glucose Oxidase/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Enzymes, Immobilized); 0 (Ferrous Compounds); 0 (Metallocenes); 9002-98-6 (Polyethyleneimine); EC 1.1.3.4 (Glucose Oxidase); IY9XDZ35W2 (Glucose); U96PKG90JQ (ferrocene) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180115 [Lr] Data última revisão: 180115 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161023 [St] Status: MEDLINE

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[PMID]: 28761341 [Au] Autor: Xia N; Liu K; Zhou Y; Li Y; Yi X [Ad] Endereço: Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang. [Ti] Título: Sensitive detection of microRNAs based on the conversion of colorimetric assay into electrochemical analysis with duplex-specific nuclease-assisted signal amplification. [So] Source: Int J Nanomedicine;12:5013-5022, 2017. [Is] ISSN: 1178-2013 [Cp] País de publicação: New Zealand [La] Idioma: eng [Ab] Resumo: miRNAs have emerged as new biomarkers for the detection of a wide variety of cancers. By employing duplex-specific nuclease for signal amplification and gold nanoparticles (AuNPs) as the carriers of detection probes, a novel electrochemical assay of miRNAs was performed. The method is based on conversion of the well-known colorimetric assay into electrochemical analysis with enhanced sensitivity. DNA capture probes immobilized on the electrode surface and ferrocene (Fc)-labeled DNA detection probes (denoted "Fc-DNA-Fc") presented in the solution induced the assembly of positively charged AuNPs on the electrode surface through the electrostatic interaction. As a result, a large number of Fc-DNA-Fc molecules were attached on the electrode surface, thus amplifying the electrochemical signal. When duplex-specific nuclease was added to recycle the process of miRNA-initiated digestion of the immobilized DNA probes, Fc-DNA-Fc-induced assembly of AuNPs on the electrode surface could not occur. This resulted in a significant fall in the oxidation current of Fc. The current was found to be inversely proportional to the concentration of miRNAs in the range of 0-25 fM, and a detection limit of 0.1 fM was achieved. Moreover, this work presents a new method for converting colorimetric assays into sensitive electrochemical analyses, and thus would be valuable for design of novel chemical/biosensors. [Mh] Termos MeSH primário: Colorimetria/métodos Técnicas Eletroquímicas/métodos MicroRNAs/análise Nanopartículas/química [Mh] Termos MeSH secundário: Técnicas Biossensoriais/métodos Sondas de DNA/química Eletrodos Compostos Ferrosos/química Ouro/química Seres Humanos Limite de Detecção Metalocenos MicroRNAs/sangue MicroRNAs/química Ácidos Nucleicos Heteroduplexes/química Sensibilidade e Especificidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA Probes); 0 (Ferrous Compounds); 0 (Metallocenes); 0 (MicroRNAs); 0 (Nucleic Acid Heteroduplexes); 7440-57-5 (Gold); U96PKG90JQ (ferrocene) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170802 [St] Status: MEDLINE [do] DOI: 10.2147/IJN.S138656

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[PMID]: 28672274 [Au] Autor: Aghbolagh MS; Khani Meynaq MY; Shimizu K; Lindholm-Sethson B [Ad] Endereço: Department of Chemistry, Umeå University, SE901 87 Umeå, Sweden. [Ti] Título: Aspects on mediated glucose oxidation at a supported cubic phase. [So] Source: Bioelectrochemistry;118:8-13, 2017 Dec. [Is] ISSN: 1878-562X [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: A supported liquid crystalline cubic phase housing glucose oxidase on an electrode surface has been suggested as bio-anode in a biofuel. The purpose of this investigation is to clarify some aspect on the mediated enzymatic oxidation of glucose in such a bio-anode where the mediator ferrocene-carboxylic acid and glucose were dissolved in the solution. The enzyme glucose oxidase was housed in the water channels of the mono-olein cubic phase. The system was investigated with cyclic voltammetry at different scan rates and the temperature was varied between 15°C and 30°C. The diffusion coefficient of the mediator and also the film resistance was estimated showing a large decrease in the mass-transport properties as the temperature was decreased. The current from mediated oxidation of glucose at the electrode surface increased with decreasing film thickness. The transport of the mediator in the cubic phase was the rate-limiting step in the overall reaction, where the oxidation of glucose took place at the outer surface of the cubic phase. [Mh] Termos MeSH primário: Biocatálise Enzimas Imobilizadas/química Enzimas Imobilizadas/metabolismo Glucose Oxidase/química Glucose Oxidase/metabolismo Glucose/química [Mh] Termos MeSH secundário: Carbono/química Ácidos Carboxílicos/química Eletroquímica Eletrodos Compostos Ferrosos/química Metalocenos Oxirredução Temperatura Ambiente [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Carboxylic Acids); 0 (Enzymes, Immobilized); 0 (Ferrous Compounds); 0 (Metallocenes); 7440-44-0 (Carbon); EC 1.1.3.4 (Glucose Oxidase); IY9XDZ35W2 (Glucose); U96PKG90JQ (ferrocene) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170704 [St] Status: MEDLINE

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[PMID]: 28645004 [Au] Autor: Ahangar LE; Mehrgardi MA [Ad] Endereço: Department of Chemistry, University of Isfahan, Isfahan 81746-73441, Iran. [Ti] Título: Amplified detection of hepatitis B virus using an electrochemical DNA biosensor on a nanoporous gold platform. [So] Source: Bioelectrochemistry;117:83-88, 2017 Oct. [Is] ISSN: 1878-562X [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: In the present study, a nanoporous gold platform was applied for the amplified detection of Hepatitis B virus (HBV) by an electrochemical DNA biosensor. Ferrocene as a redox reporter was covalently attached to the DNA probe and its electrochemical signal was recorded as the biosensor response. For real samples, DNA was firstly extracted from blood of patients and then amplified by polymerase chain reaction (PCR) for 5cycles. Sensitivity of this biosensor was enhanced by using nanoporous gold electrode, therefore this sensor can discriminate the genome of HBV in real sample with low PCR cycles. By this strategy and signal amplification using nanoporous platform and covalently attached electroactive label, the biosensor can distinguish between healthy and HBV patients with limited PCR cycles. Moreover, the errors of PCR with large cycles can be disregarded. A linear dynamic range of 0.4 to 10nmol of mutant DNA was achieved, with reliable reproducibility (RSD) 8.9%. [Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos DNA Viral/análise Ouro/química Vírus da Hepatite B/isolamento & purificação Nanoporos [Mh] Termos MeSH secundário: Sequência de Bases DNA Viral/genética Eletroquímica Eletrodos Compostos Ferrosos/química Vírus da Hepatite B/genética Metalocenos Mutação Reação em Cadeia da Polimerase Propriedades de Superfície [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Viral); 0 (Ferrous Compounds); 0 (Metallocenes); 7440-57-5 (Gold); U96PKG90JQ (ferrocene) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170624 [St] Status: MEDLINE

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[PMID]: 28583799 [Au] Autor: Hasegawa Y; Takada T; Nakamura M; Yamana K [Ad] Endereço: Department of Materials Science and Chemistry, University of Hyogo, 2167 Shosha, Himeji 671-2280, Japan. [Ti] Título: Ferrocene conjugated oligonucleotide for electrochemical detection of DNA base mismatch. [So] Source: Bioorg Med Chem Lett;27(15):3555-3557, 2017 08 01. [Is] ISSN: 1464-3405 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: We describe the synthesis, binding, and electrochemical properties of ferrocene-conjugated oligonucleotides (Fc-oligos). The key step for the preparation of Fc-oligos contains the coupling of vinylferrocene to 5-iododeoxyuridine via Heck reaction. The Fc-conjugated deoxyuridine phosphoramidite was used in the Fc-oligonucleotide synthesis. We show that thiol-modified Fc-oligos deposited onto gold electrodes possess potential ability in electrochemical detection of DNA base mismatch. [Mh] Termos MeSH primário: Pareamento Incorreto de Bases DNA/genética Desoxiuridina/análogos & derivados Técnicas Eletroquímicas/métodos Compostos Ferrosos/química Oligonucleotídeos/química [Mh] Termos MeSH secundário: Sequência de Bases DNA/química Eletrodos Ouro/química Idoxuridina/química Metalocenos Compostos de Vinila/química [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Ferrous Compounds); 0 (Metallocenes); 0 (Oligonucleotides); 0 (Vinyl Compounds); 1271-51-8 (vinylferrocene); 7440-57-5 (Gold); 9007-49-2 (DNA); LGP81V5245 (Idoxuridine); U96PKG90JQ (ferrocene); W78I7AY22C (Deoxyuridine) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 171125 [Lr] Data última revisão: 171125 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170607 [St] Status: MEDLINE

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[PMID]: 28494361 [Au] Autor: Lin KA; Lin JT [Ad] Endereço: Department of Environmental Engineering, National Chung Hsing University, 250 Kuo-Kuang Road, Taichung, Taiwan. Electronic address: linky@nchu.edu.tw. [Ti] Título: Ferrocene-functionalized graphitic carbon nitride as an enhanced heterogeneous catalyst of Fenton reaction for degradation of Rhodamine B under visible light irradiation. [So] Source: Chemosphere;182:54-64, 2017 Sep. [Is] ISSN: 1879-1298 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: To enhance degradation of Rhodamine B (RhB), a toxic xanthene dye, an iron-doped graphitic carbon nitride (CN) is prepared by establishing a covalent bond (-CN-) bridging ferrocene (Fc) and CN via a Schiff base reaction. The π-conjugation between the aromatic Fc and CN can be much enhanced by the covalent bond, thereby facilitating the bulk-to-surface charge transfer and separation as well as reversible photo-redox reactions during photocatalytic reactions. Thus, the resulting Fc-CN exhibits a much higher catalytic activity than CN to activate hydrogen peroxide (HP) for RhB degradation, because the photocatalytically generated electrons from CN can activate HP and effectively maintain the bivalence state of Fe in Fc, which also induces the activation of HP. The RhB degradation by the Fc-CN activated HP process (Fc-CN-HP) is validated to involve OH by examining the effect of radical probe agent as well as electron paramagnetic resonance (EPR) spectroscopic analysis. Fc-CN is also proven to activate HP for RhB degradation over multiple times without loss of catalytic activity. Through determining the degradation intermediates, RhB is indeed fully decomposed by Fc-CN-HP into much lower-molecular-weight organic compounds. These features indicate that Fc-functionalization can be an advantageous technique to enhance the catalytic activity of CN for activating HP. The results obtained in this study are essential to further design and utilize Fc-functionalized CN for Fenton-like reactions. The findings shown here, especially the degradation mechanism and pathway, are also quite important for treating xanthene dyes in wastewater. [Mh] Termos MeSH primário: Grafite/química Rodaminas/química Purificação da Água/métodos [Mh] Termos MeSH secundário: Catálise Corantes Recuperação e Remediação Ambiental/métodos Compostos Ferrosos Peróxido de Hidrogênio/química Ferro/química Luz Metalocenos Nitrilos Compostos Orgânicos Rodaminas/efeitos da radiação [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Coloring Agents); 0 (Fenton's reagent); 0 (Ferrous Compounds); 0 (Metallocenes); 0 (Nitriles); 0 (Organic Chemicals); 0 (Rhodamines); 534Q0F66RK (cyanogen); 7782-42-5 (Graphite); BBX060AN9V (Hydrogen Peroxide); E1UOL152H7 (Iron); K7G5SCF8IL (rhodamine B); U96PKG90JQ (ferrocene) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170512 [St] Status: MEDLINE

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[PMID]: 28482262 [Au] Autor: Asghar F; Badshah A; Lal B; Zubair S; Fatima S; Butler IS [Ad] Endereço: Coordination Chemistry Laboratory, Department of Chemistry, Quaid-i-Azam University, Islamabad 45320, Pakistan; Department of Chemistry, McGill University, Montreal, QC H3A 2K6, Canada; Department of Chemistry, University of Wah, Quaid Avenue, Wah 47000, Pakistan. [Ti] Título: Facile synthesis of fluoro, methoxy, and methyl substituted ferrocene-based urea complexes as potential therapeutic agents. [So] Source: Bioorg Chem;72:215-227, 2017 Jun. [Is] ISSN: 1090-2120 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: In the present work, the synthesis, characterization (FT-IR, multinuclear ( H and C) NMR, AAS, Raman, and elemental analysis), DNA binding (cyclic voltammetry, UV-Vis spectroscopy and viscometry), and in vitro biological assessment of nine new ferrocene-based ureas are reported. The desulphurization of ferrocenyl thioureas to the corresponding oxo analogues using aqueous sodium hydroxide and mercuric chloride led to the ferrocenyl ureas (F1-F9) in high yields. The DNA binding studies performed by cyclic voltammetry and UV-Vis spectroscopy produced results that are in close agreement with one another for the binding constants (K) and an electrostatic mode of interaction was observed. The nature and the extent of interaction with DNA was further investigated by viscometry. The DFT/B3LYP method was used to determine the charge distribution and HOMO/LUMO energies of the optimized structure. The DFT calculated HOMO and LUMO energies correlate well with the experimentally determined redox potential values. The synthesized ferrocenyl derivatives exhibited good scavenging activity against 1,1-diphenyl-2-picrylhydrazyl radical (DPPH). These complexes were also scanned for their in vitro cytotoxicity against human carcinoma cell line THP-1 (leukemia cells). The results showed a moderate level of cytotoxicity against the subjected cancer cell line as compared with the standard chemotherapeutic drug (cisplatin). [Mh] Termos MeSH primário: Antineoplásicos/farmacologia Compostos de Bifenilo/antagonistas & inibidores DNA de Neoplasias/efeitos dos fármacos Compostos Ferrosos/farmacologia Picratos/antagonistas & inibidores Ureia/farmacologia [Mh] Termos MeSH secundário: Antineoplásicos/síntese química Antineoplásicos/química Linhagem Celular Tumoral Proliferação Celular/efeitos dos fármacos Relação Dose-Resposta a Droga Compostos Ferrosos/química Seres Humanos Metalocenos Estrutura Molecular Teoria Quântica Relação Estrutura-Atividade Ureia/análogos & derivados Ureia/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents); 0 (Biphenyl Compounds); 0 (DNA, Neoplasm); 0 (Ferrous Compounds); 0 (Metallocenes); 0 (Picrates); 8W8T17847W (Urea); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl); U96PKG90JQ (ferrocene) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170509 [St] Status: MEDLINE

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[PMID]: 28273561 [Au] Autor: Pérès B; Nasr R; Zarioh M; Lecerf-Schmidt F; Di Pietro A; Baubichon-Cortay H; Boumendjel A [Ad] Endereço: Département de Pharmacochimie Moléculaire, Université Grenoble-Alpes, CNRS UMR 5063, F-38041 Grenoble, France. Electronic address: basile.peres@univ-grenoble-alpes.fr. [Ti] Título: Ferrocene-embedded flavonoids targeting the Achilles heel of multidrug-resistant cancer cells through collateral sensitivity. [So] Source: Eur J Med Chem;130:346-353, 2017 Apr 21. [Is] ISSN: 1768-3254 [Cp] País de publicação: France [La] Idioma: eng [Ab] Resumo: With the aim to develop anticancer agents acting selectively against resistant tumor cells, we investigated ferrocene embedded into chalcone, aurone and flavone skeletons. These compounds were conceived and then investigated based on the concept of collateral sensitivity, where the target is the Achilles Heel of cancer cells overexpressing the multidrug ABC transporter MRP1. The 14 synthesized compounds were evaluated for their ability to induce efflux of glutathione (GSH) from tumor cells overexpressing MRP1. When tested at 5 and 20 µM, at least one compound from each series was found to be a highly inducer of GSH efflux. The different compounds inducing a high efflux of GSH were evaluated on both sensitive and resistant cell lines, and two of them, belonging to the flavones class were found to be more cytotoxic on resistant cancer cells, with the best selectivity ratio >9.1. Our results bring chemical and biological bases for further optimization. [Mh] Termos MeSH primário: Antineoplásicos/farmacologia Resistência a Múltiplos Medicamentos/efeitos dos fármacos Compostos Ferrosos/química Flavonoides/farmacologia [Mh] Termos MeSH secundário: Antineoplásicos/química Linhagem Celular Tumoral Citotoxinas/química Citotoxinas/farmacologia Resistência a Medicamentos Antineoplásicos/efeitos dos fármacos Flavonoides/química Glutationa/metabolismo Seres Humanos Metalocenos Proteínas Associadas à Resistência a Múltiplos Medicamentos/biossíntese Sensibilidade e Especificidade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents); 0 (Cytotoxins); 0 (Ferrous Compounds); 0 (Flavonoids); 0 (Metallocenes); 0 (Multidrug Resistance-Associated Proteins); GAN16C9B8O (Glutathione); U96PKG90JQ (ferrocene); Y49M64GZ4Q (multidrug resistance-associated protein 1) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170309 [St] Status: MEDLINE

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[PMID]: 28113133 [Au] Autor: Dervisevic M; Senel M; Sagir T; Isik S [Ad] Endereço: Biotechnology Research Lab, EMC Technology Inc, ARGEM Building, Technocity, Avcilar, Istanbul 34320, Turkey. [Ti] Título: Boronic Acid vs. Folic Acid: A Comparison of the bio-recognition performances by Impedimetric Cytosensors based on Ferrocene cored dendrimer. [So] Source: Biosens Bioelectron;91:680-686, 2017 May 15. [Is] ISSN: 1873-4235 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A comparative study is reported where folic acid (FA) and boronic acid (BA) based cytosensors and their analytical performances in cancer cell detection were analyzed by using electrochemical impedance spectroscopy (EIS) method. Cytosensors were fabricated using self-assembled monolayer principle by modifying Au electrode with cysteamine (Cys) and immobilization of ferrocene cored polyamidiamine dendrimers second generation (Fc-PAMAM (G2)), after which electrodes were modified with FA and BA. Au/Fc-PAMAM(G2)/FA and Au/Fc-PAMAM(G2)/BA based cytosensors showed extremely good analytical performances in cancer cell detection with linear range of 1×10 to 1×10 cellsml , detection limit of 20cellsml with incubation time of 20min for FA based electrode, and for BA based electrode detection limit was 28cellsml with incubation time of 10min. Next to excellent analytical performances, cytosensors showed high selectivity towards cancer cells which was demonstrated in selectivity study using human embryonic kidney 293 cells (HEK 293) as normal cells and Au/Fc-PAMAM(G2)/FA electrode showed two times better selectivity than BA modified electrode. These cytosensors are promising for future applications in cancer cell diagnosis. [Mh] Termos MeSH primário: Técnicas Biossensoriais/instrumentação Dendrímeros/química Espectroscopia Dielétrica/instrumentação Compostos Ferrosos/química Ácido Fólico/química Neoplasias/diagnóstico [Mh] Termos MeSH secundário: Técnicas Biossensoriais/métodos Linhagem Celular Tumoral Espectroscopia Dielétrica/métodos Eletrodos Desenho de Equipamento Ouro/química Células HEK293 Seres Humanos Metalocenos Reprodutibilidade dos Testes [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Dendrimers); 0 (Ferrous Compounds); 0 (Metallocenes); 0 (PAMAM Starburst); 7440-57-5 (Gold); 935E97BOY8 (Folic Acid); U96PKG90JQ (ferrocene) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170124 [St] Status: MEDLINE

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[PMID]: 28110663 [Au] Autor: Zaitseva AS; Arlyapov VA; Yudina NY; Alferov SV; Reshetilov AN [Ad] Endereço: Tula State University, 92 Lenin Prosp., Tula, 300012, Russia. [Ti] Título: Use of one- and two-mediator systems for developing a BOD biosensor based on the yeast Debaryomyces hansenii. [So] Source: Enzyme Microb Technol;98:43-51, 2017 Mar. [Is] ISSN: 1879-0909 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: We investigated the use of one- and two-mediator systems in amperometric BOD biosensors (BOD, biochemical oxygen demand) based on the yeast Debaryomyces hansenii. Screening of nine mediators potentially capable of electron transfer - ferrocene, 1,1'-dimethylferrocene, ferrocenecarboxaldehyde, ferroceneacetonitrile, neutral red, 2,6-dichlorophenolindophenol, thionine, methylene blue and potassium ferricyanide - showed only ferrocene and neutral red to be efficient electron carriers for the eukaryotes studied. Two-mediator systems based on combinations of the investigated compounds were used to increase the efficiency of electron transfer. The developed two-mediator biosensors exceeded their one-mediator analogs by their characteristics. The most preferable two-mediator system for developing a BOD biosensor was a ferrocene-methylene blue combination that ensured a satisfactory long-time stability (43 days), selectivity, sensitivity (the lower limit of the determined BOD concentrations, 2.5mg О /dm ) and speed (assay time for one sample, not greater than 10min) of BOD determination. Analysis of water samples showed that the use of a ferrocene-methylene blue two-mediator system and the yeast D. hansenii enabled registration of data that highly correlated with the results of the standard method (R=0.9913). [Mh] Termos MeSH primário: Análise da Demanda Biológica de Oxigênio/métodos Técnicas Biossensoriais/métodos Debaromyces/metabolismo [Mh] Termos MeSH secundário: Transporte de Elétrons Compostos Ferrosos/metabolismo Indicadores e Reagentes/metabolismo Metalocenos Azul de Metileno/metabolismo Vermelho Neutro/metabolismo Água/análise [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Ferrous Compounds); 0 (Indicators and Reagents); 0 (Metallocenes); 059QF0KO0R (Water); 261QK3SSBH (Neutral Red); T42P99266K (Methylene Blue); U96PKG90JQ (ferrocene) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170124 [St] Status: MEDLINE

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1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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