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[PMID]:8628097
[Au] Autor:Bujía J; Wilmes E; Kastenbauer E; Gürtler L
[Ad] Endereço:Department of Otorhinolaryngology, Ludwig Maximilians University, Munich, Germany.
[Ti] Título:Influence of chemical allograft preservation procedures on the human immunodeficiency virus.
[So] Source:Laryngoscope;106(5 Pt 1):645-7, 1996 May.
[Is] ISSN:0023-852X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Since chemically preserved allogenic transplants have an established place in reconstructive procedures, the possibility of transferring the human immunodeficiency virus (HIV) with these transplants has been intensively discussed. In this study the authors obtained brain and spleen samples from six HIV-infected cadavers and preserved them with Merthiolate, Cialit, and formaldehyde. After preservation, the tissues were examined for proviral HIV-1 DNA (gag, pol, env) using the polymerase chain reaction. Proviral sequences were clearly demonstrated after the preservation procedure. The results of this study indicate that HIV remains in tissues that have been treated with Merthiolate, formaldehyde, or Cialit. Further investigations are necessary to determine if the virus is in an inactivated or activated form. It can be concluded that, because of the possible transmission of HIV by chemically preserved homografts, serologic screening of donors should be mandatory.
[Mh] Termos MeSH primário: Infecções por HIV/transmissão
HIV/isolamento & purificação
Preservação de Tecido
Transplante Homólogo
[Mh] Termos MeSH secundário: Sequência de Bases
Sangue/virologia
Encéfalo/virologia
Cartilagem/virologia
Cialit
Tecido Conjuntivo/virologia
Formaldeído
Seres Humanos
Dados de Sequência Molecular
Reação em Cadeia da Polimerase
Baço/virologia
Timerosal
Traqueia/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
16509-11-8 (Cialit); 1HG84L3525 (Formaldehyde); 2225PI3MOV (Thimerosal)
[Em] Mês de entrada:9606
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM; X
[Da] Data de entrada para processamento:960501
[St] Status:MEDLINE


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Fotocópia
[PMID]:8549246
[Au] Autor:van Rij G; Beekhuis WH; Eggink CA; Geerards AJ; Remeijer L; Pels EL
[Ad] Endereço:Department of Ophthalmology, University of Groningen, The Netherlands.
[Ti] Título:Toxic keratopathy due to the accidental use of chlorhexidine, cetrimide and cialit.
[So] Source:Doc Ophthalmol;90(1):7-14, 1995.
[Is] ISSN:0012-4486
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Due to economical reasons some ophthalmologists are using an irrigating solution made by the hospital pharmacy instead of the commercially available solutions. These irrigating solutions come in bottles which are identical to the ones used for other solutions. During the last three years bottles were accidentally mixed up five times. Consequently, bottles containing solutions such as chlorhexidine, cetrimide, chlorhexidine/centrimide and cialit solutions were used during cataract surgery. This resulted in immediate corneal edema which, in its turn resulted in a bullous keratopathy. Four patients underwent a penetrating keratoplasty. In one patient the cornea was covered with a conjunctival flap. Light microscopy of the corneas included epithelial edema, loss of keratocytes, and a disrupted and sometimes absent endothelial cell layer.
[Mh] Termos MeSH primário: Anti-Infecciosos Locais/efeitos adversos
Compostos de Cetrimônio/efeitos adversos
Clorexidina/efeitos adversos
Cialit/efeitos adversos
Córnea/efeitos dos fármacos
Doenças da Córnea/induzido quimicamente
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Extração de Catarata
Córnea/patologia
Córnea/cirurgia
Doenças da Córnea/patologia
Doenças da Córnea/cirurgia
Edema da Córnea/induzido quimicamente
Edema da Córnea/patologia
Edema da Córnea/cirurgia
Feminino
Seres Humanos
Complicações Intraoperatórias
Ceratoplastia Penetrante
Masculino
Soluções Oftálmicas
Irrigação Terapêutica
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents, Local); 0 (Cetrimonium Compounds); 0 (Ophthalmic Solutions); 16509-11-8 (Cialit); R4KO0DY52L (Chlorhexidine); Z7FF1XKL7A (cetrimonium)
[Em] Mês de entrada:9602
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:950101
[St] Status:MEDLINE


  3 / 49 MEDLINE  
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Fotocópia
[PMID]:7546680
[Au] Autor:el-Kahky M; Linthicum F; Sarwat A; el-Mehairy H; Wahba H
[Ad] Endereço:Department of Otorhinolaryngology Faculty of Medicine, Ain-Shams University, Abbassia, Cairo, Egypt.
[Ti] Título:A comparative electron microscopic study of chemical techniques for preservation of auditory ossicles as homografts.
[So] Source:Eur Arch Otorhinolaryngol;252(4):239-43, 1995.
[Is] ISSN:0937-4477
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The ultrastructure of preserved ossicles at different shelf lives was compared. Homograft ossicles were harvested and preserved in 70% alcohol, 1:5000 Cialit (sodium-2-ethyl-mercuri-mercapto-benzoxazole-5-carboxylate), and buffered 4% formaldehyde at pH 7. The ultrastructure of each ossicle was studied by both scanning and transmission electron microscopy and was compared to that of fresh controls placed in 0.9 N saline solution. The results indicated clearly the superiority of 70% alcohol as a long-term preservative and 1:5000 Cialit as a short-term preservative.
[Mh] Termos MeSH primário: Ossículos da Orelha/transplante
Preservação de Tecido/métodos
[Mh] Termos MeSH secundário: Cialit
Colágeno/ultraestrutura
Ossículos da Orelha/patologia
Etanol
Formaldeído
Sobrevivência de Enxerto/fisiologia
Seres Humanos
Microscopia Eletrônica
Microscopia Eletrônica de Varredura
Cloreto de Sódio
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
16509-11-8 (Cialit); 1HG84L3525 (Formaldehyde); 3K9958V90M (Ethanol); 451W47IQ8X (Sodium Chloride); 9007-34-5 (Collagen)
[Em] Mês de entrada:9511
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:950101
[St] Status:MEDLINE


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Fotocópia
[PMID]:8357633
[Au] Autor:Bujía J; Wilmes E; Bartual-Pastor J; Hammer C
[Ad] Endereço:Cátedra de Otorrinolaringología, Ludwig-Maximilians-Universidad de Munich.
[Ti] Título:[Comparative study of the effect of different chemical procedures on the antigenicity of allogenic transplants of the human trachea].
[Ti] Título:Estudio comparativo del efecto de distintos procedimientos químicos sobre la antigenicidad de trasplantes alógenos de tráquea humana..
[So] Source:Acta Otorrinolaringol Esp;44(3):209-16, 1993 May-Jun.
[Is] ISSN:0001-6519
[Cp] País de publicação:Spain
[La] Idioma:spa
[Ab] Resumo:Replacement of long tracheal segments poses some unsolved problems, specially the undisponibility of an adequated substitution material. This study deals with some of the immunological aspects of the transplantation and preservation of human tracheal grafts. Since immunological rejection occurs in the first place as a consequence of incompatibility for class II antigens (HLA-DR, -DP and -DQ), their presence and distribution was studied using monoclonal antibodies and the immunoperoxidase method. Class II expressing cells were recognized in the mucosa and the mixed glandular tissue. Furthermore we investigated the influence of different preservation methods on the antigenicity of tracheal allografts. The results showed a total destruction of these antigens. We can conclude that chemically preserved human tracheal allografts are immunologically suitable for reconstructive surgery of the trachea.
[Mh] Termos MeSH primário: Traqueia/imunologia
Traqueia/transplante
[Mh] Termos MeSH secundário: Cialit
Histocompatibilidade
Seres Humanos
Preservação de Órgãos
Timerosal
Transplante Homólogo/imunologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
16509-11-8 (Cialit); 2225PI3MOV (Thimerosal)
[Em] Mês de entrada:9309
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:930501
[St] Status:MEDLINE


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Fotocópia
[PMID]:1286825
[Au] Autor:Donald PJ
[Ad] Endereço:Department of Otolaryngology-Head & Neck Surgery, University of California, Davis Medical Center, Sacramento.
[Ti] Título:Homographic cartilage in facial implantation.
[So] Source:Facial Plast Surg;8(3):157-75, 1992 Jul.
[Is] ISSN:0736-6825
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Cartilagem/transplante
Face/cirurgia
Cirurgia Plástica/métodos
[Mh] Termos MeSH secundário: Absorção
Animais
Cartilagem/imunologia
Cartilagem/metabolismo
Cialit
Liofilização
Seres Humanos
Timerosal
Preservação de Tecido/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
16509-11-8 (Cialit); 2225PI3MOV (Thimerosal)
[Em] Mês de entrada:9303
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:920701
[St] Status:MEDLINE


  6 / 49 MEDLINE  
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Fotocópia
[PMID]:2243530
[Au] Autor:Bujía J; Wilmes E; Hammer C; Kastenbauer E
[Ad] Endereço:Department of Otorhinolaryngology, Ludwig-Maximilians-Universität, Munich, Federal Republic of Germany.
[Ti] Título:A comparison of class II antigenicity of human tracheal allografts stored in cialit and in merthiolate.
[So] Source:Laryngoscope;100(12):1337-40, 1990 Dec.
[Is] ISSN:0023-852X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This report deals with some of the immunological aspects of the transplantation and preservation of human tracheal grafts. The immunological behavior of the transplanted graft depends largely on the interaction of the preservatives with the tissue proteins. Using monoclonal antibodies and the immunoperoxidase staining technique we have investigated the effect of Merthiolate, Cialit, formaldehyde/Merthiolate, and formaldehyde/Cialit preservation techniques on monomorphic determinants of class II transplantation antigens in human tracheal allografts. Unpreserved grafts were found to express class II antigens. These antigens were totally destroyed after 7 days in formaldehyde and 42 days in Cialit and Merthiolate. Preservation in Cialit and Merthiolate showed a gradual disintegration of the histological structures. In contrast, buffered formaldehyde did not appear to alter the histological structure of the tracheal graft. Irrespective of the mode of preservation, the cartilaginous tissue appeared to persist virtually unaffected. No essential differences were observed between the immunological staining of tracheal grafts preserved in Cialit and Merthiolate.
[Mh] Termos MeSH primário: Antígenos HLA-D/análise
Preservação de Órgãos/métodos
Traqueia/imunologia
[Mh] Termos MeSH secundário: Cialit
Seres Humanos
Timerosal
Traqueia/citologia
Traqueia/transplante
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HLA-D Antigens); 16509-11-8 (Cialit); 2225PI3MOV (Thimerosal)
[Em] Mês de entrada:9101
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:901201
[St] Status:MEDLINE


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[PMID]:2198870
[Au] Autor:Hüttenbrink KB; Weidenfeller P
[Ad] Endereço:HNO-Universitätsklinik Münster.
[Ti] Título:[Are cialit-preserved ossicles as middle ear implants still bacteriologically defensible? Their replacement by pre-formed implants fashioned from teeth].
[Ti] Título:Sind Cialit-konservierte Ossikel als Mittelohrimplantate bakteriologisch noch vertretbar? Ihr Ersatz durch vorgefertigte Implantate aus Zähnen..
[So] Source:Laryngorhinootologie;69(6):327-32, 1990 Jun.
[Is] ISSN:0935-8943
[Cp] País de publicação:Germany
[La] Idioma:ger
[Ab] Resumo:Recent studies have demonstrated a limited bactericidal activity of Cialit in the preservation of otologic transplants. The authors therefore performed several tests following routine treatment of the grafts. First, the contamination of allogenic ossicles was studied; afterwards, the bactericidal activity of Cialit was tested against standardized germs and clinical isolates. At the highly toxic concentration of 1:2000, Cialit is bactericidal. For clinical purposes, however, the less toxic solution of 1:5000 is generally used, which is not sufficiently safe against certain resistant clinical isolates. The fact that the activity of Cialit depends on its concentration explains the controversial findings in the literature. Furthermore, it is doubtful whether a weaker solution would prevent transmission of an HIV or hepatitis infection. Therefore, the authors recommend the autoclaving of otologic allografts. This also has forensic reasons; for example if the serologic status of the donor is unknown. As ossicles become brittle under this procedure, the authors now use sculptured parts of teeth, in which the hydroxyl-apatite is much more compact. The preformed prostheses can be autoclaved without problem. More than 150 reconstructions have proved the acceptance of this cheap implant material. Furthermore, forensic problems with ossicles from corpses do not arise.
[Mh] Termos MeSH primário: Bioprótese
Cialit
Ossículos da Orelha/transplante
Compostos de Etilmercúrio
Prótese Ossicular
Infecção da Ferida Cirúrgica/microbiologia
Preservação de Tecido/métodos
[Mh] Termos MeSH secundário: Bactérias/isolamento & purificação
Técnicas Bacteriológicas
Cadáver
Ossículos da Orelha/microbiologia
Seres Humanos
Fatores de Risco
Infecção da Ferida Cirúrgica/prevenção & controle
[Pt] Tipo de publicação:COMPARATIVE STUDY; ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylmercury Compounds); 16509-11-8 (Cialit)
[Em] Mês de entrada:9009
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM; X
[Da] Data de entrada para processamento:900601
[St] Status:MEDLINE


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Fotocópia
[PMID]:2198064
[Au] Autor:Tange RA; Troost D; Limburg M
[Ad] Endereço:Department of Otolaryngology, University of Amsterdam, The Netherlands.
[Ti] Título:Progressive fatal dementia (Creutzfeldt-Jakob disease) in a patient who received homograft tissue for tympanic membrane closure.
[So] Source:Eur Arch Otorhinolaryngol;247(4):199-201, 1990.
[Is] ISSN:0937-4477
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:We report the case history of a 54-year-old man who developed a fatal neurological disorder 4 years after a successful tympanoplasty with homograft pericardium. The final diagnosis of this case was Creutzfeldt-Jakob disease. This infectious spongiform encephalopathy is probably caused by a slow virus that can be transmitted by transplantation materials. The possible accidental transmission of Creutzfeldt-Jacob disease by the use of homograft materials in otologic surgery is discussed.
[Mh] Termos MeSH primário: Síndrome de Creutzfeldt-Jakob/transmissão
Pericárdio/transplante
Timpanoplastia
[Mh] Termos MeSH secundário: Córtex Cerebral/patologia
Cialit
Síndrome de Creutzfeldt-Jakob/patologia
Seres Humanos
Masculino
Meia-Idade
Fatores de Tempo
Preservação de Tecido
Transplante Homólogo
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
16509-11-8 (Cialit)
[Em] Mês de entrada:9009
[Cu] Atualização por classe:170714
[Lr] Data última revisão:
170714
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:900101
[St] Status:MEDLINE


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Fotocópia
[PMID]:2605283
[Au] Autor:Visser CE; Boon ME; Visser PE; Kok LP
[Ad] Endereço:Department of Otorhinolaryngology, University of Amsterdam, The Netherlands.
[Ti] Título:Microwave treatment of xenogeneic cartilage transplants.
[So] Source:Biomaterials;10(8):507-10, 1989 Oct.
[Is] ISSN:0142-9612
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Human rib cartilage was irradiated with microwaves according to six different methods and transplanted into rabbits. Untreated rib cartilage preserved in Cialit served as a control. After 12 and 40 wk of implantation, the microscopic appearance of these xenogeneic cartilage transplants was given a score in comparison with the transplants preserved in Cialit. The microwave treatment of the cartilage appeared to improve the results. The Cialit-preserved transplants showed progressive resorption by macrophages with central necrosis and fragmentation, which was not present in the microwave-treated grafts. Microwaves seem to stabilize the cartilage matrix and enhance the diffusion of fixatives. Irradiation in ethanol as an immersion fluid appeared to be the best method. The results of transplantation can benefit from the use of microwaves in the preservation of the cartilage. It is argued that, in addition, microwave irradiation might be used for inactivation of human immunodeficiency virus in human cartilage used for transplantation.
[Mh] Termos MeSH primário: Cartilagem/transplante
Micro-Ondas
Preservação de Tecido/métodos
Transplante Heterólogo
[Mh] Termos MeSH secundário: Animais
Cartilagem/patologia
Cartilagem/efeitos da radiação
Cialit/administração & dosagem
Etanol/administração & dosagem
Feminino
Seres Humanos
Macrófagos/patologia
Necrose
Projetos Piloto
Coelhos
Cicatrização
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
16509-11-8 (Cialit); 3K9958V90M (Ethanol)
[Em] Mês de entrada:9002
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM; X
[Da] Data de entrada para processamento:891001
[St] Status:MEDLINE


  10 / 49 MEDLINE  
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Fotocópia
[PMID]:2504857
[Au] Autor:Adlington P; Anscombe AJ; Joshi JB
[Ti] Título:Influence of the mode of preparation on the distortion and consistency of homologous costal cartilage implants.
[So] Source:J Laryngol Otol;103(6):572-6, 1989 Jun.
[Is] ISSN:0022-2151
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nasal implants of autologous costal cartilage have an undesirable tendency to distortion. This tendency may be reduced by the process of devitalization, which occurs in the preparation of homologous costal cartilage for storage in a tissue bank. An in vitro study has been undertaken, utilizing five common methods of cartilage preparation (cialit, ethyl alcohol, formalin, glutaraldehyde and irradiation) to determine whether there is any significant variation in the degree of distortion which occurred and any variation in cartilage consistency. We conclude that all of the methods evaluated would produce banked homologous costal cartilage with an equally low incidence of distortion but implants are less readily carved from cartilage stored in cialit.
[Mh] Termos MeSH primário: Cartilagem
Preservação de Tecido/métodos
[Mh] Termos MeSH secundário: Cartilagem/efeitos da radiação
Cartilagem/transplante
Cialit
Etanol
Formaldeído
Glutaral
Seres Humanos
Costelas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
16509-11-8 (Cialit); 1HG84L3525 (Formaldehyde); 3K9958V90M (Ethanol); T3C89M417N (Glutaral)
[Em] Mês de entrada:8909
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:890601
[St] Status:MEDLINE



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