Base de dados : MEDLINE
Pesquisa : D02.691.750.740 [Categoria DeCS]
Referências encontradas : 362 [refinar]
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[PMID]:27565891
[Au] Autor:Wang S; Lv Q; Yang Y; Guo LH; Wan B; Ren X; Zhang H
[Ad] Endereço:State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, 18 Shuangqing Road, Beijing 100085, China; College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024
[Ti] Título:Arginine decarboxylase: A novel biological target of mercury compounds identified in PC12 cells.
[So] Source:Biochem Pharmacol;118:109-120, 2016 Oct 15.
[Is] ISSN:1873-2968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mercury compounds are well-known toxic environmental pollutants and potently induce severe neurotoxicological effects in human and experimental animals. Previous studies showed that one of the mechanisms of mercury compounds neurotoxicity arose from the over-activation of the N-methyl d-aspartate (NMDA)-type glutamate receptor induced by increased glutamate release. In this work, we aimed to investigate the molecular mechanisms of Hg compounds neurotoxicities by identifying their biological targets in cells. Firstly, the inhibitory effects of four Hg compounds, including three organic (methyl-, ethyl- and phenyl-mercury) and one inorganic (Hg ) Hg compounds, on the activity of arginine decarboxylase (ADC), a key enzyme in the central agmatinergic system, were evaluated. They were found to inhibit the ADC activity significantly with methylmercury (MeHg) being the strongest (IC =7.96nM). Furthermore, they showed remarkable inhibitory effects on ADC activity in PC12 cells (MeHg>EtHg>PhHg>HgCl ), and led to a marked loss in the level of agmatine, an endogenous neuromodulatory and neuroprotective agent that selectively blocks the activation of NMDA receptors. MeHg was detected in the immunoprecipitated ADC from the cells, providing unequivocal evidence for the direct binding of MeHg with ADC in the cell. Molecular dynamics simulation revealed that Hg compounds could form the coordination bond not only with cofactor PLP of ADC, but also with substrate arginine. Our finding indicated that MeHg could attenuate the neuroprotective effects of agmatine by the inhibition of ADC, a new cellular target of MeHg, which might be implicated in molecular mechanism of MeHg neurotoxicity.
[Mh] Termos MeSH primário: Carboxiliases/antagonistas & inibidores
Poluentes Ambientais/toxicidade
Inibidores Enzimáticos/toxicidade
Compostos de Metilmercúrio/toxicidade
Modelos Moleculares
Proteínas do Tecido Nervoso/antagonistas & inibidores
Neurônios/efeitos dos fármacos
[Mh] Termos MeSH secundário: Absorção Fisiológica
Agmatina/antagonistas & inibidores
Agmatina/metabolismo
Animais
Arginina/metabolismo
Sítios de Ligação
Biocatálise/efeitos dos fármacos
Carboxiliases/química
Carboxiliases/genética
Carboxiliases/metabolismo
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Complexos de Coordenação/antagonistas & inibidores
Complexos de Coordenação/química
Complexos de Coordenação/metabolismo
Descarboxilação/efeitos dos fármacos
Poluentes Ambientais/antagonistas & inibidores
Poluentes Ambientais/metabolismo
Inibidores Enzimáticos/química
Inibidores Enzimáticos/metabolismo
Cloreto Etilmercúrico/antagonistas & inibidores
Cloreto Etilmercúrico/metabolismo
Cloreto Etilmercúrico/toxicidade
Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos
Cloreto de Mercúrio/antagonistas & inibidores
Cloreto de Mercúrio/metabolismo
Cloreto de Mercúrio/toxicidade
Compostos de Metilmercúrio/antagonistas & inibidores
Compostos de Metilmercúrio/metabolismo
Simulação de Dinâmica Molecular
Proteínas do Tecido Nervoso/química
Proteínas do Tecido Nervoso/genética
Proteínas do Tecido Nervoso/metabolismo
Neurônios/enzimologia
Neurônios/metabolismo
Compostos de Fenilmercúrio/antagonistas & inibidores
Compostos de Fenilmercúrio/metabolismo
Compostos de Fenilmercúrio/toxicidade
Ratos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coordination Complexes); 0 (Environmental Pollutants); 0 (Enzyme Inhibitors); 0 (Methylmercury Compounds); 0 (Nerve Tissue Proteins); 0 (Phenylmercury Compounds); 53GH7MZT1R (Mercuric Chloride); 70J407ZL5Q (Agmatine); 94ZLA3W45F (Arginine); EC 4.1.1.- (Carboxy-Lyases); EC 4.1.1.19 (arginine decarboxylase); M04218TP6P (Ethylmercuric Chloride); RWZ4L3O1X0 (methylmercuric chloride); X0R4ES0U7Z (phenylmercuric chloride)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170501
[Lr] Data última revisão:
170501
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160828
[St] Status:MEDLINE


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[PMID]:24147942
[Au] Autor:Charoo N; Chiew M; Tay A; Lian L
[Ad] Endereço:Innovation and Development, Xepa Soul Pattinson , Melaka , Malaysia.
[Ti] Título:Temperature-mediated absorption of phenylmercuric nitrate on polyethylene and polypropylene containers in chloramphenicol eye drops.
[So] Source:Cutan Ocul Toxicol;33(3):242-6, 2014 Sep.
[Is] ISSN:1556-9535
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this work was to find the effect of temperature and manufacturing source of phenylmercuric nitrate (PMN) on PMN absorption on low-density polyethylene (LDPE) and polypropylene containers in chloramphenicol eye drops. Two factorial experiments were designed to study the effect of temperature on PMN assay in chloramphenicol eye drops stored in LDPE and prepared from two different PMN sources. PMN source had no effect on PMN assay at 2-8 °C, however at stress conditions (30 °C/75%RH) for 3 weeks, the effect of PMN source on PMN assay was found significant (p < 0.05) in formulations stored in LDPE bottles. Temperature was the major contributor to decreased PMN assay. In formulations stored in polypropylene containers, PMN source had significant effect on PMN assay at 2-8 °C and 30 °C/75%RH. Overall, new PMN and polypropylene bottles performed better. The eye drops complied with preservative efficacy test both initially and at the end of shelf life. The concentration exponent of PMN is very low and in spite of its high absorption by container/closure, PMN was still able to protect the eye drops at the end of shelf life. It can be inferred that preservative efficacy test is the better indicator of preservative activity.
[Mh] Termos MeSH primário: Anti-Infecciosos Locais/química
Anti-Infecciosos/química
Compostos de Fenilmercúrio/química
Polietileno/química
Polipropilenos/química
Conservantes Farmacêuticos/química
[Mh] Termos MeSH secundário: Absorção Fisico-Química
Antibacterianos
Cloranfenicol
Embalagem de Medicamentos
Estabilidade de Medicamentos
Soluções Oftálmicas
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Anti-Infective Agents); 0 (Anti-Infective Agents, Local); 0 (Ophthalmic Solutions); 0 (Phenylmercury Compounds); 0 (Polypropylenes); 0 (Preservatives, Pharmaceutical); 66974FR9Q1 (Chloramphenicol); 9002-88-4 (Polyethylene); W06K73CUGI (phenylmercuric nitrate, basic)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131024
[St] Status:MEDLINE
[do] DOI:10.3109/15569527.2013.837058


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[PMID]:24148384
[Au] Autor:Escudero LB; Olsina RA; Wuilloud RG
[Ad] Endereço:Laboratory of Analytical Chemistry for Research and Development (QUIANID), Instituto de Ciencias Básicas, Universidad Nacional de Cuyo, Padre J. Contreras 1300, Parque Gral. San Martín, M5502JMA Mendoza, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; Instituto de Química de San Luis, Universidad Nacional de San Luis (INQUISAL-UNSL), San Luis, Argentina.
[Ti] Título:Polymer-supported ionic liquid solid phase extraction for trace inorganic and organic mercury determination in water samples by flow injection-cold vapor atomic absorption spectrometry.
[So] Source:Talanta;116:133-40, 2013 Nov 15.
[Is] ISSN:1873-3573
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A simple and green technique named polymer-supported ionic liquid solid phase extraction (PSIL-SPE) was developed for mercury (Hg) species determination. Inorganic Hg (InHg) species was complexed with chloride ions followed by its introduction into a flow injection on-line system to quantitatively retain the anionic chlorocomplex (HgCl4(2-)) in a column packed with CYPHOS(®) IL 101-impregnated resin. The trapped InHg was then reduced with stannous chloride (SnCl2) and eluted with the same flow of reducing agent followed by cold vapor atomic absorption spectrometry (CV-AAS) detection. Organic mercury species (OrgHg) did not interact with the impregnated resin and were not retained into the column. Total concentration of OrgHg was evaluated by difference between total Hg and InHg concentration. A 95% extraction efficiency was achieved for InHg when the procedure was developed under optimal experimental conditions. The limit of detection obtained for preconcentration of 40 mL of sample was 2.4 ng L(-1) InHg. The relative standard deviation (RSD) was 2.7% (at 1 µg L(-1) InHg and n=10) calculated from the peak height of absorbance signals (Gaussian-shape and reproducible peaks). This work reports the first polymer-supported IL solid phase extraction approach implemented in a flow injection on-line system for determination of Hg species in mineral, tap and river water samples.
[Mh] Termos MeSH primário: Água Doce/química
Compostos de Metilmercúrio/isolamento & purificação
Compostos de Fenilmercúrio/isolamento & purificação
Extração em Fase Sólida/métodos
Poluentes Químicos da Água/isolamento & purificação
[Mh] Termos MeSH secundário: Concentração de Íons de Hidrogênio
Resinas de Troca Iônica/química
Limite de Detecção
Compostos Organofosforados/química
Oxirredução
Reprodutibilidade dos Testes
Espectrofotometria Atômica
Compostos de Estanho/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Ion Exchange Resins); 0 (Methylmercury Compounds); 0 (Organophosphorus Compounds); 0 (Phenylmercury Compounds); 0 (Tin Compounds); 0 (Water Pollutants, Chemical); 0 (trihexyltetradecylphosphonium chloride); 1BQV3749L5 (stannous chloride); RWZ4L3O1X0 (methylmercuric chloride); X0R4ES0U7Z (phenylmercuric chloride)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:131023
[Lr] Data última revisão:
131023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131024
[St] Status:MEDLINE


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[PMID]:23985830
[Au] Autor:Sone Y; Nakamura R; Pan-Hou H; Itoh T; Kiyono M
[Ad] Endereço:Department of Public Health and Molecular Toxicology, School of Pharmacy, Kitasato University.
[Ti] Título:Role of MerC, MerE, MerF, MerT, and/or MerP in resistance to mercurials and the transport of mercurials in Escherichia coli.
[So] Source:Biol Pharm Bull;36(11):1835-41, 2013.
[Is] ISSN:1347-5215
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:The characteristics of bacteria take up mercury into cells via membrane potential-dependent sequence-divergent members of the mercuric ion (Mer) superfamily, i.e., a periplasmic mercuric ion scavenging protein (MerP) and one or more inner membrane-spanning proteins (MerC, MerE, MerF, and MerT), which transport mercuric ions into the cytoplasm, have been applied in engineering of bioreactor used for mercurial bioremediation. We engineered bacteria to express MerC, MerE, MerF, or MerT with or without MerP to clarify their individual role and potential in transport of mercurial. By immunoblot analysis using specific polyclonal antibody, the proteins encoded by merC, merE, merF, merT or merP, were certainly expressed and identified in the membrane fraction. Bacteria expressing MerC, MerE, MerF or MerT in the absence of MerP transported significantly more C6H5Hg(I) and Hg(II) across bacterial membrane than their isogenic strain. In vivo expression of MerP in the presence of all the transporters did not cause apparent difference to the C6H5Hg(I) transport, but gives an apparently higher Hg(II) transport than that did by MerE, MerF or MerT but not by MerC. Among the four transporters studied, MerC showed more potential to transport Hg(II) across bacterial membrane than MerE, MerF and MerT. Together these findings, we demonstrated for the first time that in addition to MerE and MerT, MerF and MerC are broad-spectrum mercury transporters that mediate both Hg(II) and phenylmercury transport into cells. Our results suggested that MerC is the most efficient tool for designing mercurial bioremediation systems, because MerC is sufficient for mercurial transport into cells.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Proteínas de Transporte de Cátions/metabolismo
Escherichia coli/metabolismo
Mercúrio/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Biodegradação Ambiental
Transporte Biológico
Proteínas de Transporte de Cátions/genética
Escherichia coli/genética
Compostos de Fenilmercúrio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Cation Transport Proteins); 0 (Phenylmercury Compounds); FXS1BY2PGL (Mercury)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:131105
[Lr] Data última revisão:
131105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130830
[St] Status:MEDLINE


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[PMID]:23947701
[Au] Autor:Miranda C; Yáñez J; Contreras D; Zaror C; Mansilla HD
[Ad] Endereço:Faculty of Chemical Sciences, University of Concepción, Concepción, Chile.
[Ti] Título:Phenylmercury degradation by heterogeneous photocatalysis assisted by UV-A light.
[So] Source:J Environ Sci Health A Tox Hazard Subst Environ Eng;48(13):1642-8, 2013.
[Is] ISSN:1532-4117
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Photocatalytic degradation of phenylmercury was studied using TiO2 in aqueous suspension assisted by UV-A irradiation. Reaction conditions, such as pH and amount of TiO2 were set using a factorial design of experiments resulting in a greater influence of pH on phenylmercury degradation. Hg (II) reduction and simultaneous oxidation of aromatic group was observed. Optimum reaction conditions were obtained under nitrogen atmosphere at pH 10 and 0.35 g/L(-1) TiO2. Under these conditions almost 100% reduction of mercury was reached after 30 min UV irradiation. Total mercury reduction was achieved after 40 min reaction under saturated oxygen. Furthermore, phenol and diphenylmercury were identified as intermediate products of oxidation. It was observed that a major fraction of the reduced mercury was removed as metallic vapor by gas stripping, whereas a minor fraction was adsorbed on the catalyst surface, probably as Hg(OH)2. Under optimal conditions obtained by multivariable analysis, total mineralization of organic matter was achieved after about 60-min irradiation.
[Mh] Termos MeSH primário: Compostos de Fenilmercúrio/química
Compostos de Fenilmercúrio/efeitos da radiação
Titânio/química
Raios Ultravioleta
Poluentes Químicos da Água/química
Poluentes Químicos da Água/efeitos da radiação
[Mh] Termos MeSH secundário: Fungicidas Industriais/química
Fungicidas Industriais/efeitos da radiação
Cromatografia Gasosa-Espectrometria de Massas
Microscopia Eletrônica de Varredura
Modelos Teóricos
Fotólise
Espectrometria por Raios X
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fungicides, Industrial); 0 (Phenylmercury Compounds); 0 (Water Pollutants, Chemical); 15FIX9V2JP (titanium dioxide); D1JT611TNE (Titanium)
[Em] Mês de entrada:1403
[Cu] Atualização por classe:160526
[Lr] Data última revisão:
160526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130817
[St] Status:MEDLINE
[do] DOI:10.1080/10934529.2013.815453


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[PMID]:23773406
[Au] Autor:Brubacher JW; Owen TM; Vrahas MS
[Ad] Endereço:Department of Orthopaedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States. Electronic address: jbrubacher@partners.org.
[Ti] Título:Use of Surgilube to minimize metal debris in removal of jammed titanium locking screws.
[So] Source:Injury;44(11):1648-50, 2013 Nov.
[Is] ISSN:1879-0267
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Mh] Termos MeSH primário: Parafusos Ósseos/efeitos adversos
Fraturas do Fêmur/cirurgia
Fixação Interna de Fraturas/efeitos adversos
Compostos de Fenilmercúrio
Titânio
[Mh] Termos MeSH secundário: Placas Ósseas
Remoção de Dispositivo/métodos
Feminino
Fixação Interna de Fraturas/instrumentação
Fixação Interna de Fraturas/métodos
Seres Humanos
Lubrificantes
Masculino
Reoperação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lubricants); 0 (Phenylmercury Compounds); D1JT611TNE (Titanium); ZT1TTY3NGJ (phenylmercury borate)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130619
[St] Status:MEDLINE


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[PMID]:23748021
[Au] Autor:Doulias PT; Tenopoulou M; Raju K; Spruce LA; Seeholzer SH; Ischiropoulos H
[Ad] Endereço:Children's Hospital of Philadelphia Research Institute, Philadelphia, PA 19104, USA. Electronic address: doulias@chop.email.edu.
[Ti] Título:Site specific identification of endogenous S-nitrosocysteine proteomes.
[So] Source:J Proteomics;92:195-203, 2013 Oct 30.
[Is] ISSN:1876-7737
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Cysteine S-nitrosylation is a post-translational modification regulating protein function and nitric oxide signaling. Herein the selectivity, reproducibility, and sensitivity of a mass spectrometry-based proteomic method for the identification of endogenous S-nitrosylated proteins are outlined. The method enriches for either S-nitrosylated proteins or peptides through covalent binding of the cysteine sulfur with phenylmercury at pH=6.0. Phenylmercury reacts selectively and efficiently with S-nitrosocysteine since no reactivity can be documented for disulfides, sulfinic or sulfonic acids, S-glutathionylated, S-alkylated or S-sulfhydrylated cysteine residues. A specificity of 97±1% for the identification of S-nitrosocysteine peptides in mouse liver tissue is achieved by the inclusion of negative controls. The method enables the detection of 36 S-nitrosocysteine peptides starting with 5pmolS-nitrosocysteine/mg of total tissue protein. Both the percentage of protein molecules modified as well as the occupancy by S-nitrosylation can be determined. Overall, selective, sensitive and reproducible enrichment of S-nitrosylated proteins and peptides is achieved by the use of phenylmercury. The inclusion of appropriate negative controls secures the precise identification of endogenous S-nitrosylated sites and proteins in biological samples. BIOLOGICAL SIGNIFICANCE: The current study describes a selective, sensitive and reproducible method for the acquisition of endogenously S-nitrosylated proteins and peptides. The acquisition of endogenous S-nitrosoproteomes provides robust data that is necessary for investigating the mechanism(s) of S-nitrosylation in vivo, the factors that govern its selectivity, the dependency of the modification on different isoforms of nitric oxide synthases (NOS), as well as the physiological functions of this protein modification. This article is part of a Special Issue entitled: Posttranslational Protein modifications in biology and Medicine.
[Mh] Termos MeSH primário: Cisteína/análogos & derivados
Espectrometria de Massas/métodos
Óxido Nítrico Sintase/metabolismo
Processamento de Proteína Pós-Traducional
Proteoma/metabolismo
S-Nitrosotióis/metabolismo
[Mh] Termos MeSH secundário: Animais
Bovinos
Cisteína/química
Cisteína/metabolismo
Camundongos
Óxido Nítrico Sintase/química
Compostos de Fenilmercúrio/química
Proteoma/química
Coelhos
S-Nitrosotióis/química
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Phenylmercury Compounds); 0 (Proteome); 0 (S-Nitrosothiols); 926P2322P4 (S-nitrosocysteine); EC 1.14.13.39 (Nitric Oxide Synthase); K848JZ4886 (Cysteine); X0R4ES0U7Z (phenylmercuric chloride)
[Em] Mês de entrada:1406
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130611
[St] Status:MEDLINE


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[PMID]:23102555
[Au] Autor:Xu Y; He Y; Li X; Gao C; Zhou L; Sun S; Pang G
[Ad] Endereço:Laboratory of Ocular Pharmacology, Henan Eye Institute, Department of Ophthalmology, Henan Provincial People's Hospital, Zhengzhou, 450003, Henan, China. xuyan990301@yahoo.com.cn
[Ti] Título:Antifungal effect of ophthalmic preservatives phenylmercuric nitrate and benzalkonium chloride on ocular pathogenic filamentous fungi.
[So] Source:Diagn Microbiol Infect Dis;75(1):64-7, 2013 Jan.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In the present study, the antifungal effects of phenylmercuric nitrate and benzalkonium chloride versus those of natamycin and ketoconazole were assessed against 216 filamentous fungi isolates from cases of fungal keratitis. They included 112 Fusarium isolates, 94 Aspergillus isolates, and 10 Alternaria alternata isolates. The strains were tested by broth dilution antifungal susceptibility testing of filamentous fungi approved by the Clinical and Laboratory Standards Institute M38-A document. The results showed that the MIC(50) values of phenylmercuric nitrate were 0.0156, 0.0156, and 0.0313 µg/mL for Fusarium spp., Aspergillus spp., and A. alternata, respectively. The MIC(90) values of phenylmercuric nitrate were 0.0313, 0.0313, and 0.0313 µg/mL for Fusarium spp., Aspergillus spp., and A. alternata, respectively. The MIC(50) values of benzalkonium chloride were 16, 32, and 8 µg/mL for Fusarium spp., Aspergillus spp., and A. alternata, respectively. The MIC(90) values of benzalkonium chloride were 32, 32, and 16 µg/mL for Fusarium spp., Aspergillus spp., and A. alternata, respectively. The study indicates that phenylmercuric nitrate has considerable antifungal activity and its effect is significantly superior to those of benzalkonium chloride, natamycin, and ketoconazole against ocular pathogenic filamentous fungi in vitro, deserving further investigation for treating fungal keratitis as a main drug.
[Mh] Termos MeSH primário: Alternaria/efeitos dos fármacos
Aspergillus/efeitos dos fármacos
Compostos de Benzalcônio/farmacologia
Fusarium/efeitos dos fármacos
Soluções Oftálmicas/química
Compostos de Fenilmercúrio/farmacologia
Conservantes Farmacêuticos/farmacologia
[Mh] Termos MeSH secundário: Alternaria/isolamento & purificação
Antifúngicos/farmacologia
Aspergillus/isolamento & purificação
Fusarium/isolamento & purificação
Seres Humanos
Ceratite/microbiologia
Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Benzalkonium Compounds); 0 (Ophthalmic Solutions); 0 (Phenylmercury Compounds); 0 (Preservatives, Pharmaceutical); W06K73CUGI (phenylmercuric nitrate, basic)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121030
[St] Status:MEDLINE


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[PMID]:23057960
[Au] Autor:Patel AN; Vargas V; Revello P; Bull DA
[Ad] Endereço:Division of Cardiothoracic Surgery, University of Utah, Salt Lake City, UT, USA. Amit.patel@hsc.utah.edu
[Ti] Título:Mesenchymal stem cell population isolated from the subepithelial layer of umbilical cord tissue.
[So] Source:Cell Transplant;22(3):513-9, 2013.
[Is] ISSN:1555-3892
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The therapeutic use of stem cells to treat diseases and injuries is a promising tool in regenerative medicine. The umbilical cord provides a rich source of stem cells; we have previously reported a population of stem cells isolated from Wharton's jelly. In this report, we aimed to isolate a novel cell population that was different than those found in Wharton's jelly. We isolated stem cells from the subepithelial layer of the umbilical cord; the cells could be expanded for greater than 90 population doubling and had mesenchymal stem cell characteristics, expressing CD9, SSEA4, CD44, CD90, CD166, CD73, and CD146 but were negative for STRO-1. The cells can be directionally differentiated and undergo osteo-, chondro-, adipo-, and cardiogenesis. In addition, we have identified for the first time that mesenchymal stem cells isolated from umbilical cord can produce microvesicles, termed exosomes. This is the first report describing a stem cell population isolated from the subepithelial layer of the umbilical cord. Given the growth capacity, multilineage potential, and most importantly the low levels of HLA-ABC, we propose that this novel cell isolated from the subepithelial layer of umbilical cord is an ideal candidate for allogeneic cell-based therapy.
[Mh] Termos MeSH primário: Células Mesenquimais Estromais/citologia
Cordão Umbilical/citologia
[Mh] Termos MeSH secundário: Adipogenia
Antígenos CD/metabolismo
Antígenos de Superfície/metabolismo
Diferenciação Celular
Linhagem da Célula
Proliferação Celular
Separação Celular
Células Cultivadas
Condrogênese
Dexametasona/análogos & derivados
Dexametasona/metabolismo
Combinação de Medicamentos
Células Epiteliais/citologia
Seres Humanos
Cariotipagem
Células Mesenquimais Estromais/metabolismo
Osteogênese
Compostos de Fenilmercúrio/metabolismo
Antígenos Embrionários Estágio-Específicos/metabolismo
Cordão Umbilical/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, CD); 0 (Antigens, Surface); 0 (Drug Combinations); 0 (Phenylmercury Compounds); 0 (STRO-1 antigen, human); 0 (Stage-Specific Embryonic Antigens); 0 (stage-specific embryonic antigen-4); 59271-40-8 (exosterol); 7S5I7G3JQL (Dexamethasone)
[Em] Mês de entrada:1309
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121013
[St] Status:MEDLINE
[do] DOI:10.3727/096368912X655064


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[PMID]:23153091
[Au] Autor:Liu Z; Zhu Z; Zheng H; Hu S
[Ad] Endereço:State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, China.
[Ti] Título:Plasma jet desorption atomization-atomic fluorescence spectrometry and its application to mercury speciation by coupling with thin layer chromatography.
[So] Source:Anal Chem;84(23):10170-4, 2012 Dec 04.
[Is] ISSN:1520-6882
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A novel plasma jet desorption atomization (PJDA) source was developed for atomic fluorescence spectrometry (AFS) and coupled on line with thin layer chromatography (TLC) for mercury speciation. An argon dielectric barrier discharge plasma jet, which is generated inside a 300 µm quartz capillary, interacts directly with the sample being analyzed and is found to desorb and atomize surface mercury species rapidly. The effectiveness of this PJDA surface sampling technique was demonstrated by measuring AFS signals of inorganic Hg(2+), methylmercury (MeHg), and phenylmercury (PhHg) deposited directly on TLC plate. The detection limits of the proposed PJDA-AFS method for inorganic Hg(2+), MeHg, and PhHg were 0.51, 0.29, and 0.34 pg, respectively, and repeatability was 4.7%, 2.2%, and 4.3% for 10 pg Hg(2+), MeHg, and PhHg. The proposed PJDA-AFS was also successfully coupled to TLC for mercury speciation. Under optimized conditions, the measurements of mercury dithizonate (Hg-D), methylmercury dithizonate (MeHg-D), and phenylmercury dithizonate (PhHg-D) could be achieved within 3 min with detection limits as low as 8.7 pg. The combination of TLC with PJDA-AFS provides a simple, cost-effective, relatively high-throughput way for mercury speciation.
[Mh] Termos MeSH primário: Cromatografia em Camada Delgada/métodos
Ditizona/análise
Mercúrio/análise
Compostos de Metilmercúrio/análise
Compostos de Fenilmercúrio/análise
Espectrometria de Fluorescência/métodos
[Mh] Termos MeSH secundário: Análise de Injeção de Fluxo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Methylmercury Compounds); 0 (Phenylmercury Compounds); 60-10-6 (Dithizone); C60TQU15XY (dimethyl mercury); FXS1BY2PGL (Mercury)
[Em] Mês de entrada:1311
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121117
[St] Status:MEDLINE
[do] DOI:10.1021/ac3028504



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