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[PMID]:28456703
[Au] Autor:Bi W; Li B; Song J; Hong Y; Zhang X; Liu H; Lu H; Zhou T; Cao J
[Ad] Endereço:School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang Province, China.
[Ti] Título:Antimicrobial susceptibility and mechanisms of fosfomycin resistance in extended-spectrum ß-lactamase-producing Escherichia coli strains from urinary tract infections in Wenzhou, China.
[So] Source:Int J Antimicrob Agents;50(1):29-34, 2017 Jul.
[Is] ISSN:1872-7913
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Fosfomycin in combination with various antibiotics represents an excellent clinically efficacious regimen for the treatment of urinary tract infections (UTIs) caused by extended-spectrum ß-lactamase (ESBL)-producing Escherichia coli. Underlying mechanisms of fosfomycin resistance remain largely uncharacterised. To investigate the antibacterial efficacy of fosfomycin against ESBL-producing E. coli, 356 non-repetitive ESBL-producing E. coli clinical isolates were collected from urine specimens from patients with UTI in Wenzhou, China, from January 2011 to December 2015. Antimicrobial sensitivity testing indicated that 6.7% (24/356) of the ESBL-producing E. coli strains were resistant to fosfomycin. The fosA3 gene encoding a fosfomycin-modifying enzyme was detected in 20 isolates by PCR and sequencing, alone or in combination with other ESBL determinants. Conjugation experiments and Southern blotting demonstrated that 70% (14/20) of the fosA3-positive isolates possessed transferable plasmids (ca. 54.2 kb) co-harbouring the ESBL resistance gene bla and the fosfomycin resistance gene fosA3. Among the four fosfomycin-resistant fosA3-negative E. coli isolates, three contained amino acid substitutions (Ile28Asn and Phe30Leu in MurA and Leu297Phe in GlpT). The results indicate that presence of the fosA3 gene is the primary mechanism of fosfomycin resistance in ESBL-producing E. coli isolates in Wenzhou, China. In addition, a plasmid (ca. 54.2 kb) co-harbouring fosA3 and bla genes is horizontally transferable. Furthermore, a low degree of homology in the fosfomycin-resistant E. coli was confirmed using multilocus sequence typing (MLST), suggesting that there is no obvious phenomenon of clonal dissemination.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana
Infecções por Escherichia coli/microbiologia
Escherichia coli/efeitos dos fármacos
Fosfomicina/farmacologia
Infecções Urinárias/microbiologia
beta-Lactamases/secreção
[Mh] Termos MeSH secundário: Southern Blotting
China
Conjugação Genética
Escherichia coli/enzimologia
Escherichia coli/isolamento & purificação
Proteínas de Escherichia coli/genética
Transferência Genética Horizontal
Seres Humanos
Testes de Sensibilidade Microbiana
Plasmídeos/análise
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
beta-Lactamases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Escherichia coli Proteins); 0 (FosA(3) protein, E coli); 2N81MY12TE (Fosfomycin); EC 3.5.2.6 (beta-Lactamases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


  2 / 1725 MEDLINE  
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[PMID]:29198700
[Au] Autor:Citak F; Ghai I; Rosenkötter F; Benier L; Winterhalter M; Wagner R
[Ad] Endereço:Department of Life Sciences and Chemistry, key, 28719 Bremen, Germany.
[Ti] Título:Probing transport of fosfomycin through substrate specific OprO and OprP from Pseudomonas aeruginosa.
[So] Source:Biochem Biophys Res Commun;495(1):1454-1460, 2018 01 01.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Increasing antimicrobial drug resistance is a global threat especially with respect to Gram-negative bacteria. The low permeability of the bacterial outer cell wall has been identified as an important bottleneck that prevents a sufficient antibacterial effect to be achieved at low doses of the antibiotics. In particular, the outer membrane permeability for negatively charged molecules of the clinical important ESKAPE bacterium Pseudomonas aeruginosa is determined by the low conductance porins OprO and OprP Here we show that the alternative phosphonic-acid antibiotic fosfomycin is highly permeable through the OprO and OprP channels. For this, we applied an electrophysiological zero-current assay using concentration gradients of fosfomycin under tri-ionic conditions to quantify flux of fosfomycin through OprO and OprP. Our analyzes show that OprO, and to a lesser degree OprP, have unexpected very high permeability to fosfomycin, so the antibiotic should be a potentially excellent alternative choice for the control of Pseudomonas aeruginosa infections.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Permeabilidade da Membrana Celular/fisiologia
Fosfomicina/farmacocinética
Porinas/metabolismo
Pseudomonas aeruginosa/metabolismo
[Mh] Termos MeSH secundário: Análise do Fluxo Metabólico/métodos
Técnicas de Sonda Molecular
Especificidade por Substrato/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (OprO protein, Pseudomonas aeruginosa); 0 (Porins); 0 (oprP protein, Pseudomonas aeruginosa); 2N81MY12TE (Fosfomycin)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180105
[Lr] Data última revisão:
180105
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


  3 / 1725 MEDLINE  
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[PMID]:27773366
[Au] Autor:Adeyemi CM; Faridoon; Isaacs M; Mnkandhla D; Hoppe HC; Krause RW; Kaye PT
[Ad] Endereço:Department of Chemistry, Rhodes University, Grahamstown 6140, South Africa.
[Ti] Título:Synthesis and antimalarial activity of N-benzylated (N-arylcarbamoyl)alkylphosphonic acid derivatives.
[So] Source:Bioorg Med Chem;24(23):6131-6138, 2016 12 01.
[Is] ISSN:1464-3391
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A series of novel and readily accessible N-benzylated (N-arylcarbamoyl)alkylphosphonate esters and related compounds have been prepared as potential antimalarial agents. Bioassays reveal that some of these compounds exhibit promising activity against Plasmodium falciparum, and exhibit no significant growth inhibition of HeLa cells.
[Mh] Termos MeSH primário: Amidas/farmacologia
Antimaláricos/farmacologia
Organofosfonatos/farmacologia
[Mh] Termos MeSH secundário: Aldose-Cetose Isomerases/antagonistas & inibidores
Amidas/síntese química
Amidas/toxicidade
Antimaláricos/síntese química
Antimaláricos/toxicidade
Fosfomicina/análogos & derivados
Fosfomicina/farmacologia
Células HeLa
Seres Humanos
Organofosfonatos/síntese química
Organofosfonatos/toxicidade
Plasmodium falciparum/efeitos dos fármacos
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amides); 0 (Antimalarials); 0 (Organophosphonates); 2N81MY12TE (Fosfomycin); 66508-32-5 (3-(N-acetyl-N-hydroxy)aminopropylphosphonic acid); EC 1.1.1.267 (1-deoxy-D-xylulose 5-phosphate reductoisomerase); EC 5.3.1.- (Aldose-Ketose Isomerases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171207
[Lr] Data última revisão:
171207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  4 / 1725 MEDLINE  
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[PMID]:28952700
[Au] Autor:Zaitsev AV; Kolontarev KB
[Ad] Endereço:Department of Urology, A.I. Evdokimov Moscow State University of Medicine and Dentistry, Moscow, Russia.
[Ti] Título:[The role of fosfomycin in the management of urinary tract infections].
[So] Source:Urologiia;(4):91-96, 2017 Sep.
[Is] ISSN:1728-2985
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The article analyses major guidelines on the management of uncomplicated cystitis. The authors outline and substantiate the role of fosfomycin in the management of urinary tract infections. They provide current evidence on the resistance of uropathogens, including those producing extended-spectrum -lactamase. Combination therapy (fosfomycin + -lactams or aminoglycosides) is often used to treat infections caused by multiresistant microorganisms. The possibility of using fosfomycin for perioperative prophylaxis of UTI during endourologic operations and transrectal prostate biopsy is shown.
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Infecções Bacterianas/tratamento farmacológico
Fosfomicina/uso terapêutico
Infecções Urinárias/tratamento farmacológico
[Mh] Termos MeSH secundário: Sinergismo Farmacológico
Quimioterapia Combinada
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 2N81MY12TE (Fosfomycin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE


  5 / 1725 MEDLINE  
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[PMID]:28885139
[Au] Autor:Huang L; Hu YY; Zhang R
[Ad] Endereço:Department of Clinical Microbiology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, PR China.
[Ti] Título:Prevalence of fosfomycin resistance and plasmid-mediated fosfomycin-modifying enzymes among carbapenem-resistant Enterobacteriaceae in Zhejiang, China.
[So] Source:J Med Microbiol;66(9):1332-1334, 2017 Sep.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two hundred and thirty-three nonduplicated clinical isolates of carbapenem-resistant Enterobacteriaceae were collected from four hospitals in Zhejiang, China. 45.1 % (105/233) strains were resistant to fosfomycin, among which plasmid-mediated fosfomycin-modifying enzymes fosA, fosA2, fosA3 and fosA5 were positive, and the other fos genes were negative. 80 % (12/15) Enterobacter cloacae isolates were positive for fosA. 100 % (73/73) Klebsiella pneumoniae isolates were positive for fosA5. A conjugation experiment indicated that fosfomycin resistance could be transferred to an Escherichia coli recipient strain successfully. Fosfomycin still exhibits partial activity in carbapenem-resistant Enterobacteriaceae, especially carbapenem-resistant Escherichia coli. To our knowledge, plasmid-mediated fosfomycin-modifying enzymes account for the dominance in the carbapenem-resistant Enterobacteriaceae. Therefore, we need to pay attention to the plasmid-mediated fosfomycin-modifying enzymes fosA and fosA5 in Enterobacter cloacae and K. pneumoniae to prevent clonal dissemination in China.
[Mh] Termos MeSH primário: Carbapenêmicos/farmacologia
Farmacorresistência Bacteriana Múltipla/genética
Enterobacter cloacae/efeitos dos fármacos
Escherichia coli/efeitos dos fármacos
Fosfomicina/farmacologia
Klebsiella pneumoniae/efeitos dos fármacos
Plasmídeos/genética
[Mh] Termos MeSH secundário: China
Enterobacter cloacae/genética
Enterobacter cloacae/isolamento & purificação
Infecções por Enterobacteriaceae/tratamento farmacológico
Infecções por Enterobacteriaceae/microbiologia
Escherichia coli/genética
Escherichia coli/isolamento & purificação
Proteínas de Escherichia coli/genética
Transferência Genética Horizontal/genética
Seres Humanos
Klebsiella pneumoniae/genética
Klebsiella pneumoniae/isolamento & purificação
Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbapenems); 0 (Escherichia coli Proteins); 0 (FosA(3) protein, E coli); 0 (fosA5 protein, E coli); 2N81MY12TE (Fosfomycin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000578


  6 / 1725 MEDLINE  
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[PMID]:28759841
[Au] Autor:Wijma RA; Bahmany S; Wilms EB; van Gelder T; Mouton JW; Koch BCP
[Ad] Endereço:Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands. Electronic address: r.wijma@erasmusmc.nl.
[Ti] Título:A fast and sensitive LC-MS/MS method for the quantification of fosfomycin in human urine and plasma using one sample preparation method and HILIC chromatography.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1061-1062:263-269, 2017 Sep 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Fosfomycin is an old antibiotic that is increasingly prescribed because of emergence of the antibiotic resistance and the growing incidence of multi-drug resistant infections. Surprisingly, little is known about its pharmacokinetics (PK) and the pharmacodynamics (PD). Quantification of fosfomycin in both urine and plasma provides insight into the PK/PD characteristics of fosfomycin, which is crucial for the optimization of the therapy and the prevention of the emergence of resistance. An analytical method is therefore needed for the quantification of fosfomycin in both urine and plasma. A fast and sensitive tandem mass spectrometry method in combination with HILIC chromatography for the quantification of fosfomycin with a universal sample preparation method for urine and plasma was developed and validated according to FDA guidelines. The universal sample preparation method only requires 100µL of a sample, the addition of the internal standard fosfomycin-13C benzylamine and an ultrafiltration step. The method is applicable for the concentration range of 0.75-375mg/L (R of 0.9998 in both matrices) encompassing the clinically relevant concentration range based on the susceptibility of possible (uro)pathogens in the clinical setting. The validation results for urine and plasma for all QC levels, were <2.1% and <3.2% for accuracy, <1.5% and <1.7% for within day precision and <5.0% and <3.8% for between day precision, respectively. No matrix effects were encountered and the total recovery in urine and plasma was high (102.5% and 99.4%). Prepared samples were stable at 4°C and 15°C for at least 72h and stored samples at -80°C were stable for at least 6 months. Selectivity and sensitivity were confirmed and no carry-over was observed. The method was successfully applied in two pharmacokinetic studies in healthy volunteers and patients respectively.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Fosfomicina/sangue
Fosfomicina/urina
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Feminino
Fosfomicina/química
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Limite de Detecção
Modelos Lineares
Masculino
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
2N81MY12TE (Fosfomycin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170801
[St] Status:MEDLINE


  7 / 1725 MEDLINE  
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[PMID]:28709014
[Au] Autor:El-Najjar N; Jantsch J; Gessner A
[Ad] Endereço:Institute of Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg, Germany; Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Regensburg, Germany. Electronic address: Nahed.El-Najjar@klinik.uni-regensburg.de.
[Ti] Título:A rapid liquid chromatography-tandem mass spectrometry for the quantification of Fosfomycin in plasma, urine, and aqueous fluids.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1061-1062:57-64, 2017 Sep 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A simple and fast ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the analysis of Fosfomycin in different matrices (human plasma/urine, and aqueous fluid) has been established and validated. Sample cleanup consists, depending on the matrix used, on protein precipitation or dilution with methanol containing isotopically labeled Fosfomycin as internal standard. Compounds, separated on a Luna Omega PS C column, were detected in multiple reactions monitoring in negative ion mode using API4000 system. With a total run time of 2min and using low volume of samples (plasma: 10µl, urine: 2µl, and aqueous fluid: 5µl), the covered ranges were: plasma (12.5-800µg/mL), urine (62.5-4000µg/mL), and aqueous fluid (1-160µg/mL). The method proved to be precise and accurate. The inaccuracy and imprecision in each matrix at the four tested quality controls including the lower limit of quantification were:: plasma (≤ 6.5%, ≤ 8%), urine (≤ 5.8%, ≤ 6.3%), and aqueous fluid (≤ 10.6%, ≤12%). The method is fast and robust which makes it relevant for pharmacokinetic studies and therapeutic drug monitoring. The appropriateness of the developed method in clinical application is also confirmed.
[Mh] Termos MeSH primário: Antibacterianos/análise
Cromatografia Líquida de Alta Pressão/métodos
Fosfomicina/análise
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/farmacocinética
Monitoramento de Medicamentos
Estabilidade de Medicamentos
Fosfomicina/química
Fosfomicina/farmacocinética
Seres Humanos
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 2N81MY12TE (Fosfomycin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE


  8 / 1725 MEDLINE  
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[PMID]:28678474
[Au] Autor:Sato S; Kudo F; Kim SY; Kuzuyama T; Eguchi T
[Ad] Endereço:Department of Chemistry, Tokyo Institute of Technology , 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551, Japan.
[Ti] Título:Methylcobalamin-Dependent Radical SAM C-Methyltransferase Fom3 Recognizes Cytidylyl-2-hydroxyethylphosphonate and Catalyzes the Nonstereoselective C-Methylation in Fosfomycin Biosynthesis.
[So] Source:Biochemistry;56(28):3519-3522, 2017 Jul 18.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A methylcobalamin (MeCbl)-dependent radical S-adenosyl-l-methionine (SAM) methyltransferase Fom3 was found to catalyze the C-methylation of cytidylyl-2-hydroxyethylphosphonate (HEP-CMP) to give cytidylyl-2-hydroxypropylphosphonate (HPP-CMP), although it was originally proposed to catalyze the C-methylation of 2-hydroxyethylphosphonate to give 2-hydroxypropylphosphonate in the biosynthesis of a unique C-P bond containing antibiotic fosfomycin in Streptomyces. Unexpectedly, the Fom3 reaction product from HEP-CMP was almost a 1:1 diastereomeric mixture of HPP-CMP, indicating that the C-methylation is not stereoselective. Presumably, only the CMP moiety of HEP-CMP is critical for substrate recognition; on the other hand, the enzyme does not fix the 2-hydroxy group of the substrate and either of the prochiral hydrogen atoms at the C2 position can be abstracted by the 5'-deoxyadenosyl radical generated from SAM to form the substrate radical intermediates, which react with MeCbl to afford the corresponding products. This strict substrate recognition mechanism with no stereoselectivity of a MeCbl-dependent radical SAM methyltransferase is remarkable in natural product biosynthetic chemistry, because such a hidden clue for selective substrate recognition is likely to be found in the other biosynthetic pathways.
[Mh] Termos MeSH primário: Fosfomicina/metabolismo
Metiltransferases/metabolismo
Organofosfonatos/metabolismo
Streptomyces/enzimologia
Vitamina B 12/análogos & derivados
[Mh] Termos MeSH secundário: Vias Biossintéticas
Citidina Monofosfato/metabolismo
Metilação
S-Adenosilmetionina/metabolismo
Streptomyces/metabolismo
Especificidade por Substrato
Vitamina B 12/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2-hydroxyethyl phosphonate); 0 (Organophosphonates); 2N81MY12TE (Fosfomycin); 7LP2MPO46S (S-Adenosylmethionine); BR1SN1JS2W (mecobalamin); EC 2.1.1.- (Methyltransferases); EC 2.1.1.12 (methionine S-methyltransferase); F469818O25 (Cytidine Monophosphate); P6YC3EG204 (Vitamin B 12)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170706
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00472


  9 / 1725 MEDLINE  
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[PMID]:28408469
[Au] Autor:Errington J
[Ad] Endereço:Centre for Bacterial Cell Biology, Newcastle University, Newcastle-upon-Tyne NE1 7RU, U.K. jeff.errington@newcastle.ac.uk.
[Ti] Título:Cell wall-deficient, L-form bacteria in the 21st century: a personal perspective.
[So] Source:Biochem Soc Trans;45(2):287-295, 2017 Apr 15.
[Is] ISSN:1470-8752
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The peptidoglycan (PG) cell wall is a defining feature of the bacteria. It emerged very early in evolution and must have contributed significantly to the success of these organisms. The wall features prominently in our thinking about bacterial cell function, and its synthesis involves the action of several dozen proteins that are normally essential for viability. Surprisingly, it turns out to be relatively simple to generate bacterial genetic variants called L-forms that completely lack PG. They grow robustly provided that lack of the cell wall is compensated for by an osmoprotective growth medium. Although their existence has been noted and studied on and off for many decades, it is only recently that modern molecular and cellular methods have been applied to L-forms. We used as an experimental model to understand the molecular basis for the L-form switch. Key findings included the discovery that L-forms use an unusual blebbing, or tubulation and scission mechanism to proliferate. This mechanism is completely independent of the normal FtsZ-based division machinery and seems to require only an increased rate of membrane synthesis, leading to an increased surface area-to-volume ratio. Antibiotics that block cell wall precursor synthesis, such as phosphomycin, efficiently induce the L-form switch without the need for genetic change. The same antibiotics turned out to induce a similar L-form switch in a wide range of bacteria, including , in which we showed that proliferation was again FtsZ-independent. Aside from further basic science, future work on L-forms is likely to focus on their possible role in chronic or recurrent infections, their use as a model in studies of the origins of life, and possibly, biotechnological applications.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Proteínas do Citoesqueleto/metabolismo
Fosfomicina/farmacologia
Formas L/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Bacillus subtilis/efeitos dos fármacos
Bacillus subtilis/crescimento & desenvolvimento
Bacillus subtilis/metabolismo
Escherichia coli/efeitos dos fármacos
Escherichia coli/crescimento & desenvolvimento
Escherichia coli/metabolismo
Formas L/metabolismo
Peptidoglicano/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Cytoskeletal Proteins); 0 (FtsZ protein, Bacteria); 0 (Peptidoglycan); 2N81MY12TE (Fosfomycin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE
[do] DOI:10.1042/BST20160435


  10 / 1725 MEDLINE  
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[PMID]:28379732
[Au] Autor:Jiang W; Men S; Kong L; Ma S; Yang Y; Wang Y; Yuan Q; Cheng G; Zou W; Wang H
[Ad] Endereço:1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University , Chengdu, China .
[Ti] Título:Prevalence of Plasmid-Mediated Fosfomycin Resistance Gene fosA3 Among CTX-M-Producing Escherichia coli Isolates from Chickens in China.
[So] Source:Foodborne Pathog Dis;14(4):210-218, 2017 Apr.
[Is] ISSN:1556-7125
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to investigate the prevalence of fosfomycin resistance gene fosA3 and characterize plasmids harboring fosA3 among CTX-M-producing Escherichia coli from chickens in China. A total of 234 CTX-M-producing E. coli isolates collected from chickens from 2014 to 2016 were screened for the presence of plasmid-mediated fosfomycin resistance genes (fosA, fosA3, and fosC2). Clonal relatedness of fosA3-positive isolates was determined by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The genetic environment of fosA3 was analyzed by polymerase chain reaction (PCR) mapping. Plasmids were studied by using conjugation experiments, PCR-based replicon typing and plasmid MLST. Sixty-four (27.4%) fosA3-positive E. coli isolates were identified in this study. The gene bla (31/64) was predominant among these strains, followed by bla (18/64) and bla (14/64). Various PFGE patterns and sequence types (STs) indicated that these isolates were clonally unrelated. Seven different genetic environments of fosA3 were identified and two new combinations (ISEcp1-bla -ΔIS903D-IS26-fosA3-orf1-orf2-Δorf3-IS26 and IS26-ISEcp1-bla -orf477-bla -IS26-fosA3-orf1-orf2-Δorf3-IS26) were discovered for the first time. Conjugation experiments were successful for 47 isolates and 33 transconjugants harbored a single plasmid. Plasmids carrying fosA3 belonged to incompatibility group IncFII (17/33), IncI1 (2/33), IncHI2 (3/33), and IncB/O (1/33). F33:A-:B- plasmids carrying bla , IncHI2/ST3 plasmids carrying bla , and F2:A-:B-plasmids carrying bla were found in E. coli isolates from different provinces. Our results revealed a considerable prevalence of fosA3 gene among CTX-M-producing E. coli with clonal diversity from chickens in China. The transmission of different kinds of plasmids is responsible for the dissemination of fosA3 in chicken farms in China.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Galinhas/microbiologia
Farmacorresistência Bacteriana Múltipla/genética
Escherichia coli/genética
Fosfomicina/farmacologia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
China
DNA Bacteriano/isolamento & purificação
Eletroforese em Gel de Campo Pulsado
Escherichia coli/isolamento & purificação
Proteínas de Escherichia coli/genética
Proteínas de Escherichia coli/metabolismo
Testes de Sensibilidade Microbiana
Tipagem de Sequências Multilocus
Plasmídeos/genética
Replicon
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (DNA, Bacterial); 0 (Escherichia coli Proteins); 0 (FosA(3) protein, E coli); 2N81MY12TE (Fosfomycin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1089/fpd.2016.2230



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