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[PMID]: | 28456303 |
[Au] Autor: | Han Y; Song S; Wu H; Zhang J; Ma E |
[Ad] Endereço: | Institute of Applied Biology, Shanxi University, 92 Wucheng Road, Taiyuan, Shanxi 030006, China; School of Life Science, Shanxi University, 92 Wucheng Road, Taiyuan, Shanxi 030006, China; Shanxi Key Laboratory of Integrated Pest Management in Agriculture, 92 Wucheng Road, Taiyuan, Shanxi 030006, Chi |
[Ti] Título: | Antioxidant enzymes and their role in phoxim and carbaryl stress in Caenorhabditis elegans. |
[So] Source: | Pestic Biochem Physiol;138:43-50, 2017 May. | [Is] ISSN: | 1095-9939 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Pesticide exposure can induce oxidative stress and cause changes to antioxidant enzymes in living organisms. In the present study, the effects of phoxim (an organophosphorus insecticide) and carbaryl (a carbamate insecticide) on antioxidant enzyme activity and gene expression were investigated in the model organism Caenorhabditis elegans. The results show that phoxim exposure can induce superoxide dismutase (SOD) and catalase (CAT) activities and decrease glutathione peroxidase (GPx) activity at lower concentrations. The expression levels of sod-3, sod-5, ctl-1, gpx-6, and gpx-8 were up-regulated after treatment with phoxim. The mRNA expression levels of sod-5, ctl-1 and gpx-6 were increased approximately 70-, 170- and 130-fold, respectively, in the 0.25mM treatment group compared to the control group. Carbaryl exposure decreased SOD activity and induced CAT and GPx activities. The addition of carbaryl up-regulated the expression of sod-5, ctl-1, ctl-3 and gpx-8. Specifically, ctl-1 expression increased approximately 10-fold, and gpx-8 expression increased <30-fold in the 0.5mM treatment group relative to the control group. The transcript level of sod-5 increased >20-fold, and ctl-3 increased approximately 10-fold in the 1mM treatment group. The functions of the antioxidant enzymes during oxidative stress caused by the two insecticides were investigated using deletion mutants. The LC values phoxim for the of sod-3 (tm760), sod-5 (tm1146), ctl-1 (ok1242), ctl-3 (ok2042) and gpx-8 (tm2108) mutant strains were lower than those observed for the N2 strain. The LC values of carbaryl for the ctl-1 (ok1242), ctl-3 (ok2042) and gpx-6 (tm2535) deletion mutant strains decreased in comparison to the N2 strain. The results suggest that these two insecticides caused oxidative stress and changed altered the antioxidant enzyme activities and their gene expressions in C. elegans. The sod-3, sod-5, ctl-1, ctl-3, gpx-6, and gpx-8 encoding enzymes may play roles in defending cells from oxidative stress caused by these two insecticides. |
[Mh] Termos MeSH primário: |
Antioxidantes/metabolismo Caenorhabditis elegans/efeitos dos fármacos Carbaril/toxicidade Inseticidas/toxicidade Compostos Organotiofosforados/toxicidade Estresse Fisiológico/efeitos dos fármacos
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[Mh] Termos MeSH secundário: |
Animais Caenorhabditis elegans/enzimologia Regulação Enzimológica da Expressão Gênica
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Antioxidants); 0 (Insecticides); 0 (Organothiophosphorus Compounds); 6F5V775VPO (phoxim); R890C8J3N1 (Carbaryl) |
[Em] Mês de entrada: | 1711 |
[Cu] Atualização por classe: | 171128 |
[Lr] Data última revisão:
| 171128 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170501 |
[St] Status: | MEDLINE |
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