Base de dados : MEDLINE
Pesquisa : D02.806 [Categoria DeCS]
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[PMID]:28468657
[Au] Autor:Pei JP; Fan LH; Nan K; Li J; Dang XQ; Wang KZ
[Ad] Endereço:Department of Orthopaedics, the Second Affiliated Hospital of Xi'an Jiaotong University, No. 157 Xiwu Road, Xi'an, 710004, Shaanxi Province, People's Republic of China.
[Ti] Título:HSYA alleviates secondary neuronal death through attenuating oxidative stress, inflammatory response, and neural apoptosis in SD rat spinal cord compression injury.
[So] Source:J Neuroinflammation;14(1):97, 2017 May 03.
[Is] ISSN:1742-2094
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hydroxysafflor yellow A (HSYA) is a major active component of yellow pigment extracted from safflowers; this compound possesses potent neuroprotective effects both in vitro and in vivo. However, underlying mechanism of HSYA is not fully elucidated. The present study investigated the protective effects of HSYA in rat spinal cord compression injury model and related mechanisms involved. METHODS: Sprague-Dawley rats were divided as Sham, Control, and HSYA groups (n = 30 per group). Spinal cord injury (SCI) model was induced by application of vascular clips (force of 50 g, 1 min) to the dura at T9-T10 level of vertebra. Injured animals were administered with either HSYA (8 mg/kg at 1 and 6 h after injury, then 14 mg/kg, for a total of 7 days at 24-h time intervals) or equal volume of saline by intraperitoneal injection. RESULTS: From this experiment, we discovered that SCI in rats resulted in severe trauma, which is characterized by tissue damage, lipid peroxidation, neutrophil infiltration, inflammation mediator release, and neuronal apoptosis. However, HSYA treatment significantly reduced the following: (1) degree of tissue injury (histological score) and edema; (2) neutrophil infiltration (myeloperoxidase activity); (3) oxidative stress (superoxide dismutase, malondialdehyde, and nitric oxide); (4) pro-inflammatory cytokine expression (tumor necrosis factor-α, interleukin-6, inducible nitric oxide synthase, cyclooxygenase-2); (5) nuclear factor-κB activation; (6) apoptosis (terminal deoxynucleotidyl transferase dUTP nick end labeling staining and cysteine-aspartic protease-3 activity). Moreover, in a separate set of experiments, we clearly demonstrated that HSYA treatment significantly ameliorated recovery of limb function (as evaluated by Basso, Beattie, and Bresnahan behavioral recovery scores). CONCLUSIONS: Treatment with HSYA restrains development of oxidative stress, inflammation response, and apoptotic events associated with SCI of rats, demonstrating that HSYA is a potential neuroprotectant for human SCI therapy.
[Mh] Termos MeSH primário: Apoptose/fisiologia
Chalcona/análogos & derivados
Mediadores da Inflamação/metabolismo
Neurônios/metabolismo
Estresse Oxidativo/fisiologia
Quinonas/uso terapêutico
Compressão da Medula Espinal/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Morte Celular/efeitos dos fármacos
Morte Celular/fisiologia
Chalcona/farmacologia
Chalcona/uso terapêutico
Mediadores da Inflamação/antagonistas & inibidores
Masculino
Neurônios/efeitos dos fármacos
Neurônios/patologia
Estresse Oxidativo/efeitos dos fármacos
Pigmentos Biológicos/farmacologia
Pigmentos Biológicos/uso terapêutico
Quinonas/farmacologia
Ratos
Ratos Sprague-Dawley
Compressão da Medula Espinal/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Inflammation Mediators); 0 (Pigments, Biological); 0 (Quinones); 146087-19-6 (hydroxysafflor yellow A); 5S5A2Q39HX (Chalcone)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1186/s12974-017-0870-1


  2 / 8357 MEDLINE  
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[PMID]:29223152
[Au] Autor:Cherepanov DA; Milanovsky GE; Petrova AA; Tikhonov AN; Semenov AY
[Ad] Endereço:Lomonosov Moscow State University, Belozersky Institute of Physico-Chemical Biology, Moscow, 119992, Russia. tscherepanov@gmail.com.
[Ti] Título:Electron Transfer through the Acceptor Side of Photosystem I: Interaction with Exogenous Acceptors and Molecular Oxygen.
[So] Source:Biochemistry (Mosc);82(11):1249-1268, 2017 Nov.
[Is] ISSN:1608-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This review considers the state-of-the-art on mechanisms and alternative pathways of electron transfer in photosynthetic electron transport chains of chloroplasts and cyanobacteria. The mechanisms of electron transport control between photosystems (PS) I and II and the Calvin-Benson cycle are considered. The redistribution of electron fluxes between the noncyclic, cyclic, and pseudocyclic pathways plays an important role in the regulation of photosynthesis. Mathematical modeling of light-induced electron transport processes is considered. Particular attention is given to the electron transfer reactions on the acceptor side of PS I and to interactions of PS I with exogenous acceptors, including molecular oxygen. A kinetic model of PS I and its interaction with exogenous electron acceptors has been developed. This model is based on experimental kinetics of charge recombination in isolated PS I. Kinetic and thermodynamic parameters of the electron transfer reactions in PS I are scrutinized. The free energies of electron transfer between quinone acceptors A /A in the symmetric redox cofactor branches of PS I and iron-sulfur clusters F , F , and F have been estimated. The second-order rate constants of electron transfer from PS I to external acceptors have been determined. The data suggest that byproduct formation of superoxide radical in PS I due to the reduction of molecular oxygen in the A site (Mehler reaction) can exceed 0.3% of the total electron flux in PS I.
[Mh] Termos MeSH primário: Transporte de Elétrons
Complexo de Proteína do Fotossistema I/metabolismo
[Mh] Termos MeSH secundário: Cloroplastos/química
Cloroplastos/metabolismo
Cianobactérias/química
Cianobactérias/metabolismo
Proteínas com Ferro-Enxofre/metabolismo
Cinética
Modelos Químicos
Oxigênio/metabolismo
Quinonas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Iron-Sulfur Proteins); 0 (Photosystem I Protein Complex); 0 (Quinones); S88TT14065 (Oxygen)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1134/S0006297917110037


  3 / 8357 MEDLINE  
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[PMID]:28742971
[Au] Autor:Scharko NK; Martin ET; Losovyj Y; Peters DG; Raff JD
[Ad] Endereço:School of Public and Environmental Affairs, Indiana University , 1315 East 10th Street, Bloomington, Indiana 47405, United States.
[Ti] Título:Evidence for Quinone Redox Chemistry Mediating Daytime and Nighttime NO -to-HONO Conversion on Soil Surfaces.
[So] Source:Environ Sci Technol;51(17):9633-9643, 2017 Sep 05.
[Is] ISSN:1520-5851
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Humic acid (HA) is thought to promote NO conversion to nitrous acid (HONO) on soil surfaces during the day. However, it has proven difficult to identify the reactive sites in natural HA substrates. The mechanism of NO reduction on soil surrogates composed of HA and clay minerals was studied by use of a coated-wall flow reactor and cavity-enhanced spectroscopy. Conversion of NO to HONO in the dark was found to be significant and correlated to the abundance of C-O moieties in HA determined from the X-ray photoelectron spectra of the C 1s region. Twice as much HONO was formed when NO reacted with HA that was photoreduced by irradiation with UV-visible light compared to the dark reaction; photochemical reactivity was correlated to the abundance of C═O moieties rather than C-O groups. Bulk electrolysis was used to generate HA in a defined reduction state. Electrochemically reduced HA enhanced NO -to-HONO conversion by a factor of 2 relative to non-reduced HA. Our findings suggest that hydroquinones and benzoquinones, which are interchangeable via redox equilibria, contribute to both thermal and photochemical HONO formation. This conclusion is supported by experiments that studied NO reactivity on mineral surfaces coated with the model quinone, juglone. Results provide further evidence that redox-active sites on soil surfaces drive ground-level NO -to-nitrite conversion in the atmospheric boundary layer throughout the day, while amphoteric mineral surfaces promote the release of nitrite formed as gaseous HONO.
[Mh] Termos MeSH primário: Ácido Nitroso
Quinonas/química
[Mh] Termos MeSH secundário: Nitritos
Oxirredução
Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitrites); 0 (Quinones); 0 (Soil); T2I5UM75DN (Nitrous Acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171218
[Lr] Data última revisão:
171218
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1021/acs.est.7b01363


  4 / 8357 MEDLINE  
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[PMID]:28951607
[Au] Autor:Sahu AK; Quadri SR; Agasar D; Ruwaili JA; Jun-Li W; Dastager SG
[Ad] Endereço:Academy of Scientific and Innovative Research (AcSIR), and NCIM Resource Center, CSIR-National Chemical Laboratory, NCIM Resource Center, Pune, India.
[Ti] Título:Allostreptomyces indica sp. nov., isolated from India.
[So] Source:J Antibiot (Tokyo);70(10):1000-1003, 2017 Oct.
[Is] ISSN:0021-8820
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:A novel actinobacterium, designated strain YIM 75704 , was isolated from a limestone quarry located at Gulbarga, Karnataka, India. The novel strain has showed typical morphological and chemotaxonomic characteristics of the family Streptomycetaceae. Comparison of 16S rRNA gene sequences indicated that this strain represents a novel member of the family Streptomycetaceae and exhibited 99.0% 16S rRNA gene sequence similarities with the type species of the recently described novel genus Allostreptomyces, that is, Allostreptomyces psammosilenae, whereas other species of Streptomyces were below 95% sequence similarity. The cell hydrolysates contained the LL-isomer of diaminopimelic acid and the predominant quinones were MK-9 (H , H and H ). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylinositolmannosides and three unknown phospholipids. The DNA G+C content was 75.0 mol%. A polyphasic study of the strain with morphological, phenotypic, phylogenetic and with DNA-DNA hybridization evidence with related members showed that this strain represents novel species of Allostreptomyces for which the name Allostreptomyces indica sp. nov., is proposed. The type strain is YIM 75704 (= DSM 41985 =CCTCC AA 209051 = NCIM 5485 ).
[Mh] Termos MeSH primário: Microbiologia Ambiental
Streptomycetaceae/classificação
Streptomycetaceae/isolamento & purificação
[Mh] Termos MeSH secundário: Composição de Bases
Parede Celular/química
Análise por Conglomerados
Citosol/química
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Ácido Diaminopimélico/análise
Índia
Hibridização de Ácido Nucleico
Fosfolipídeos/análise
Filogenia
Quinonas/análise
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Streptomycetaceae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (Phospholipids); 0 (Quinones); 0 (RNA, Ribosomal, 16S); 583-93-7 (Diaminopimelic Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE
[do] DOI:10.1038/ja.2017.82


  5 / 8357 MEDLINE  
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[PMID]:28882484
[Au] Autor:Suto Y; Sato M; Fujimori K; Kitabatake S; Okayama M; Ichikawa D; Matsushita M; Yamagiwa N; Iwasaki G; Kiuchi F; Hattori Y
[Ad] Endereço:Faculty of Pharmacy, Takasaki University of Health and Welfare, Gunma 370-0033, Japan. Electronic address: ysuto@takasaki-u.ac.jp.
[Ti] Título:Synthesis and biological evaluation of the natural product komaroviquinone and related compounds aiming at a potential therapeutic lead compound for high-risk multiple myeloma.
[So] Source:Bioorg Med Chem Lett;27(19):4558-4563, 2017 10 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Alternatives of treatments for multiple myeloma (MM) have become increasingly available with the advent of new drugs such as proteasome inhibitors, thalidomide derivatives, histone deacetylase inhibitors, and antibody drugs. However, high-risk MM cases that are refractory to novel drugs remain, and further optimization of chemotherapeutics is urgently needed. We had achieved asymmetric total synthesis of komaroviquinone, which is a natural product from the plant Dracocephalum komarovi. Similar to several leading antitumor agents that have been developed from natural compounds, we describe the antitumor activity and cytotoxicity of komaroviquinone and related compounds in bone marrow cells. Our data suggested that komaroviquinone-related agents have potential as starting compounds for anticancer drug development.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Produtos Biológicos/farmacologia
Diterpenos/farmacologia
Lamiaceae/química
Mieloma Múltiplo/tratamento farmacológico
Quinonas/farmacologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos Fitogênicos/síntese química
Antineoplásicos Fitogênicos/química
Produtos Biológicos/síntese química
Produtos Biológicos/química
Células da Medula Óssea/efeitos dos fármacos
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Diterpenos/síntese química
Diterpenos/química
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Seres Humanos
Camundongos
Estrutura Molecular
Mieloma Múltiplo/patologia
Quinonas/síntese química
Quinonas/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Biological Products); 0 (Diterpenes); 0 (Quinones); 0 (komaroviquinone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE


  6 / 8357 MEDLINE  
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[PMID]:28847921
[Au] Autor:Jiang T; Guo X; Yan J; Zhang Y; Wang Y; Zhang M; Sheng B; Ma C; Xu P; Gao C
[Ad] Endereço:State Key Laboratory of Microbial Technology, Shandong University, Jinan, People's Republic of China.
[Ti] Título:A Bacterial Multidomain NAD-Independent d-Lactate Dehydrogenase Utilizes Flavin Adenine Dinucleotide and Fe-S Clusters as Cofactors and Quinone as an Electron Acceptor for d-Lactate Oxidization.
[So] Source:J Bacteriol;199(22), 2017 Nov 15.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bacterial membrane-associated NAD-independent d-lactate dehydrogenase (Fe-S d-iLDH) oxidizes d-lactate into pyruvate. A sequence analysis of the enzyme reveals that it contains an Fe-S oxidoreductase domain in addition to a flavin adenine dinucleotide (FAD)-containing dehydrogenase domain, which differs from other typical d-iLDHs. Fe-S d-iLDH from KT2440 was purified as a His-tagged protein and characterized in detail. This monomeric enzyme exhibited activities with l-lactate and several d-2-hydroxyacids. Quinone was shown to be the preferred electron acceptor of the enzyme. The two domains of the enzyme were then heterologously expressed and purified separately. The Fe-S cluster-binding motifs predicted by sequence alignment were preliminarily verified by site-directed mutagenesis of the Fe-S oxidoreductase domain. The FAD-containing dehydrogenase domain retained 2-hydroxyacid-oxidizing activity, although it decreased compared to the full Fe-S d-iLDH. Compared to the intact enzyme, the FAD-containing dehydrogenase domain showed increased catalytic efficiency with cytochrome as the electron acceptor, but it completely lost the ability to use coenzyme Q Additionally, the FAD-containing dehydrogenase domain was no longer associated with the cell membrane, and it could not support the utilization of d-lactate as a carbon source. Based on the results obtained, we conclude that the Fe-S oxidoreductase domain functions as an electron transfer component to facilitate the utilization of quinone as an electron acceptor by Fe-S d-iLDH, and it helps the enzyme associate with the cell membrane. These functions make the Fe-S oxidoreductase domain crucial for the d-lactate utilization function of Fe-S d-iLDH. Lactate metabolism plays versatile roles in most domains of life. Lactate utilization processes depend on certain enzymes to oxidize lactate to pyruvate. In recent years, novel bacterial lactate-oxidizing enzymes have been continually reported, including the unique NAD-independent d-lactate dehydrogenase that contains an Fe-S oxidoreductase domain besides the typical flavin-containing domain (Fe-S d-iLDH). Although Fe-S d-iLDH is widely distributed among bacterial species, the investigation of it is insufficient. Fe-S d-iLDH from KT2440, which is the major d-lactate-oxidizing enzyme for the strain, might be a representative of this type of enzyme. A study of it will be helpful in understanding the detailed mechanisms underlying the lactate utilization processes.
[Mh] Termos MeSH primário: Flavina-Adenina Dinucleotídeo/metabolismo
Proteínas com Ferro-Enxofre/metabolismo
Lactato Desidrogenases/genética
Lactato Desidrogenases/metabolismo
Ácido Láctico/metabolismo
Quinonas/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Coenzimas
Citocromos c/metabolismo
Elétrons
Lactato Desidrogenases/isolamento & purificação
Mutagênese Sítio-Dirigida
NAD/metabolismo
Oxirredução
Pseudomonas putida/enzimologia
Ubiquinona/análogos & derivados
Ubiquinona/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Coenzymes); 0 (Iron-Sulfur Proteins); 0 (Quinones); 0U46U6E8UK (NAD); 1339-63-5 (Ubiquinone); 146-14-5 (Flavin-Adenine Dinucleotide); 33X04XA5AT (Lactic Acid); 9007-43-6 (Cytochromes c); EC 1.1.- (Lactate Dehydrogenases); EC 1.1.1.28 (D-lactate dehydrogenase); EJ27X76M46 (coenzyme Q10)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE


  7 / 8357 MEDLINE  
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[PMID]:28829027
[Au] Autor:Liu Y; Lai Q; Xu XW; Wu YH; Cheng H; Zhang XH; Wang L; Shao Z
[Ad] Endereço:1​State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian
[Ti] Título:Reclassification of Xuhuaishuia manganoxidans Wang et al. 2015 as a later heterotypic synonym of Brevirhabdus pacifica Wu et al. 2015 and emendation of the species description.
[So] Source:Int J Syst Evol Microbiol;67(8):3095-3098, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A polyphasic taxonomic study was undertaken to clarify the exact position of type strain DY6-4T of Xuhuaishuia manganoxidans. A combination of physiological properties of X. manganoxidans DY6-4T was consistent with those of type strain 22DY15T of Brevirhabdus pacifica. The 16S rRNA gene sequence analyses indicated that X. manganoxidans DY6-4T and B. pacifica 22DY15T shared 100 % similarity and formed a monophyletic group. The close relationship between the two strains was underpinned by the results of chemotaxonomic characteristics, including the fatty acids, quinone and polar lipids. The digital DNA-DNA hybridization and average nucleotide identity values between the two strains were 99.90 and 99.98 %, respectively. Based on these results, we propose that Xuhuaishuia manganoxidans is a later heterotypic synonym of Brevirhabdus pacifica.
[Mh] Termos MeSH primário: Filogenia
Rhodobacteraceae/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Quinonas/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Quinones); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002097


  8 / 8357 MEDLINE  
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[PMID]:28820113
[Au] Autor:Meng YC; Liu HC; Kang YQ; Zhou YG; Cai M
[Ad] Endereço:1​China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
[Ti] Título:Blastomonas marina sp. nov., a bacteriochlorophyll-containing bacterium isolated from seawater.
[So] Source:Int J Syst Evol Microbiol;67(8):3015-3019, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-negative, facultatively anaerobic, dark-yellow-pigmented bacterium, named SSR2A-4-2T, was isolated from coastal water in the East China Sea. Cells were ovoid or short rods with peritrichous flagella and contained carotenoid in addition to bacteriochlorophyll a pigment. A phylogenetic dendrogram based on 16S rRNA gene sequences showed that strain SSR2A-4-2T formed a distinct clade with members of the genus Blastomonas, with Blastomonasnatatoria EY 4220T (=DSM 3183T) (similarity 95.6 %), Blastomonasursincola KR-99T (=DSM 9006T) (95.5 %) and Blastomonasaquatica PE4-5T (=JCM 30179T) (94.8 %) as its closest phylogenetic relatives. Q-10 was the predominant respiratory quinone. The major fatty acids were summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c), summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), C17 : 1ω6c and C18 : 1ω7c 11-methyl. The polar lipids contained diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, sphingoglycolipid, four unknown glycolipids and one unknown lipid. The DNA G+C content was 65.1 mol%. On the basis of the evidence presented in this study, strain SSR2A-4-2T represents a novel species of the genus Blastomonas, for which the name Blastomonas marina sp. nov. is proposed, with strain SSR2A-4-2T (=CGMCC 1.15297T=DSM 103453T) as the type strain.
[Mh] Termos MeSH primário: Bacterioclorofila A/química
Filogenia
Água do Mar/microbiologia
Sphingomonadaceae/classificação
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
China
DNA Bacteriano/genética
Ácidos Graxos/química
Fosfolipídeos/química
Pigmentação
Quinonas
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Sphingomonadaceae/genética
Sphingomonadaceae/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacteriochlorophyll A); 0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (Quinones); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002070


  9 / 8357 MEDLINE  
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[PMID]:28753517
[Au] Autor:Almansour AI; Arumugam N; Suresh Kumar R; Mahalingam SM; Sau S; Bianchini G; Menéndez JC; Altaf M; Ghabbour HA
[Ad] Endereço:Department of Chemistry, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
[Ti] Título:Design, synthesis and antiproliferative activity of decarbonyl luotonin analogues.
[So] Source:Eur J Med Chem;138:932-941, 2017 Sep 29.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:A small library of benzimidazole-fused pyrrolo[3,4-b]quinoline has been synthesized from readily available benzimidazole 2-carbaldehyde and various substituted arylamines in good to excellent yields utilizing an intramolecular Povarov reaction catalyzed by boron trifluoride diethyl etharate as the key final step. The compounds thus synthesized can be considered as decarbonyl analogues of the anticancer alkaloid luotonin A and were evaluated in a DNA relaxation assay for their ability to inhibit human topoisomerase I. Interestingly, two of the compounds showed a remarkable activity that is comparable to that of the standard drug camptothecin. The compounds were also evaluated for their cytotoxic effect in four highly aggressive human cancer cell lines, namely KB, MDA-MB231 (breast), LNCap (prostate), and HT1080 (fibrosarcoma). Some of the compounds obtained showed promising cytotoxicities for these four cell lines.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Desenho de Drogas
Pirróis/farmacologia
Quinonas/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos/síntese química
Antineoplásicos/química
Proliferação Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Ensaios de Seleção de Medicamentos Antitumorais
Seres Humanos
Estrutura Molecular
Pirróis/síntese química
Pirróis/química
Quinonas/síntese química
Quinonas/química
Relação Estrutura-Atividade
Células Tumorais Cultivadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Pyrroles); 0 (Quinones); 0 (luotonin A)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170729
[St] Status:MEDLINE


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Fotocópia
[PMID]:28739694
[Au] Autor:Bavelloni A; Focaccia E; Piazzi M; Errani C; Blalock W; Faenza I
[Ad] Endereço:Laboratory of Musculoskeletal Cell Biology, Rizzoli Orthopedic Institute, Bologna, Italy.
[Ti] Título:Cell Cycle Arrest and Apoptosis Induced by Kinamycin F in Human Osteosarcoma Cells.
[So] Source:Anticancer Res;37(8):4103-4109, 2017 08.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIM: Kinamycin F is a bacterial metabolite which contains an unusual and potentially reactive diazo group that is known for its ability to inhibit cell growth. In this study, the potential anti-tumor activity of kinamycin F was investigated in three human osteosarcoma cell lines, MG-63, U-2 OS and HOS as an antitumor agent with a potentially novel target. MATERIALS AND METHODS: Proliferation and cell viability were measured in three human osteosarcoma cell lines by commercially available kits. We also evaluated the effects of the drug on cell cycle progression using the Muse™ Cell Analyzer. Caspase-3 activity was determined by a fluorometric EnzChek assay kit. Finally, following treatment with kinamycin F the protein levels of cyclin D3, cyclin A and cdK-2 were examined. RESULTS: Kinamycin F induced a concentration-dependent cell death in all the three cell lines. Flow cytometry revealed that kinamycin F treatment at 1 µM concentration significantly increased the cell population in the G /M-phase (60-65%). Kinamycin F activated caspase 3 in all the three cell lines, clearly demonstrating that the growth inhibitory effect of kinamycin F can be attributed to apoptosis induction. Finally, kinamycin F suppressed osteosarcoma cell proliferation affecting cyclin A and D3 expression. CONCLUSION: Understanding the mechanism by which kinamycin F exerts its ability to inhibit cell growth may be a step forward in the development of new therapeutic strategies for the treatment of OS.
[Mh] Termos MeSH primário: Proliferação Celular/efeitos dos fármacos
Osteossarcoma/tratamento farmacológico
[Mh] Termos MeSH secundário: Antineoplásicos/administração & dosagem
Apoptose/efeitos dos fármacos
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Fase G2/efeitos dos fármacos
Seres Humanos
Osteossarcoma/patologia
Quinonas/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Quinones); 50556-18-8 (kinamycin F)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE



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