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Pesquisa : D02.886.030.215 [Categoria DeCS]
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[PMID]:28274460
[Au] Autor:Dorraji PS; Jalali F
[Ad] Endereço:Department of Chemistry, Razi University, 67149 Kermanshah, Iran.
[Ti] Título:Electrochemical fabrication of a novel ZnO/cysteic acid nanocomposite modified electrode and its application to simultaneous determination of sunset yellow and tartrazine.
[So] Source:Food Chem;227:73-77, 2017 Jul 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new nanocomposite (ZnO/Cysteic acid) was deposited on glassy carbon electrode by cyclic voltammetry. Uniform deposition of the nanocomposite was observed by scanning electron microscopy. The electron transfer characteristics of two food additives, sunset yellow and tartrazine, were greatly improved on the modified electrode. The prepared electrode was used in the sensitive simultaneous determination of sunset yellow and tartrazine by differential pulse voltammetry. Linear calibration curves were obtained in the concentration ranges of 0.1-3.0, and 0.07-1.86µM, and detection limits of 0.03 and 0.01µM for sunset yellow and tartrazine, respectively. The proposed method was evaluated by determination of the dyes in processed soft drinks with satisfactory results (recovery>95% and RSD%<5%).
[Mh] Termos MeSH primário: Compostos Azo/análise
Corantes/análise
Ácido Cisteico/química
Técnicas Eletroquímicas/métodos
Aditivos Alimentares/análise
Nanocompostos/química
Tartrazina/análise
[Mh] Termos MeSH secundário: Eletrodos
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azo Compounds); 0 (Coloring Agents); 0 (Food Additives); A3OGP4C37W (Cysteic Acid); H77VEI93A8 (FD & C Yellow No. 6); I753WB2F1M (Tartrazine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE


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[PMID]:26907334
[Au] Autor:Wolber FM; McGrath M; Jackson F; Wylie K; Broomfield A
[Ad] Endereço:School of Food and Nutrition, Massey University, Palmerston North 4442, New Zealand. f.m.wolber@massey.ac.nz.
[Ti] Título:Cysteic Acid in Dietary Keratin is Metabolized to Glutathione and Liver Taurine in a Rat Model of Human Digestion.
[So] Source:Nutrients;8(2):104, 2016 Feb 19.
[Is] ISSN:2072-6643
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Poultry feathers, consisting largely of keratin, are a low-value product of the poultry industry. The safety and digestibility of a dietary protein produced from keratin (KER) was compared to a cysteine-supplemented casein-based diet in a growing rat model for four weeks. KER proved to be an effective substitute for casein at 50% of the total dietary protein, with no changes in the rats' food intake, weight gain, organ weight, bone mineral density, white blood cell counts, liver glutathione, or blood glutathione. Inclusion of KER in the diet reduced total protein digestibility from 94% to 86% but significantly increased total dietary cysteine uptake and subsequent liver taurine levels. The KER diet also significantly increased caecum weight and significantly decreased fat digestibility, resulting in a lower proportion of body fat, and induced a significant increase in blood haemoglobin. KER is therefore a safe and suitable protein substitute for casein, and the cysteic acid in keratin is metabolised to maintain normal liver and blood glutathione levels.
[Mh] Termos MeSH primário: Ácido Cisteico/metabolismo
Proteínas na Dieta/metabolismo
Digestão
Glutationa/metabolismo
Queratinas/metabolismo
Fígado/metabolismo
Taurina/metabolismo
[Mh] Termos MeSH secundário: Tecido Adiposo/metabolismo
Animais
Composição Corporal
Caseínas
Ceco/efeitos dos fármacos
Ácido Cisteico/farmacologia
Cisteína/administração & dosagem
Cisteína/metabolismo
Dieta
Gorduras na Dieta/metabolismo
Proteínas na Dieta/química
Proteínas na Dieta/farmacologia
Hemoglobinas/metabolismo
Seres Humanos
Queratinas/química
Queratinas/farmacologia
Masculino
Modelos Animais
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Caseins); 0 (Dietary Fats); 0 (Dietary Proteins); 0 (Hemoglobins); 1EQV5MLY3D (Taurine); 68238-35-7 (Keratins); A3OGP4C37W (Cysteic Acid); GAN16C9B8O (Glutathione); K848JZ4886 (Cysteine)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE


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[PMID]:26051677
[Au] Autor:Kamkaew A; Burgess K
[Ad] Endereço:Department of Chemistry, Texas A&M University, Box 30012, College Station, TX 77842, USA. burgess@tamu.edu.
[Ti] Título:Aza-BODIPY dyes with enhanced hydrophilicity.
[So] Source:Chem Commun (Camb);51(53):10664-7, 2015 Jul 07.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Attempts to make a diamino disulfonic acid derivative of an aza-BODIPY showed it was difficult to add BF2 to a disulfonated azadipyrromethene, and sulfonation of an aza-BODIPY resulted in loss of the BF2 fragment. We conclude the electron-deficient character of aza-BODIPY dyes destabilizes them relative to BODIPY dyes. Consequently, sulfonation of the aza-BODIPY core is not a viable strategy to increase water solubility. This assertion was indirectly supported via stability studies of a BODIPY and an aza-BODIPY in aqueous media. To afford the desired compound type, an aza-BODIPY with two amino and two sulfonic acid groups was prepared via modification of the aryl substituents with cysteic acid.
[Mh] Termos MeSH primário: Compostos Aza/química
Compostos de Boro/química
Corantes Fluorescentes/química
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Ácido Cisteico/química
Corantes Fluorescentes/síntese química
Corantes Fluorescentes/metabolismo
Interações Hidrofóbicas e Hidrofílicas
Camundongos
Microscopia de Fluorescência
Ácidos Sulfônicos/química
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene); 0 (Aza Compounds); 0 (Boron Compounds); 0 (Fluorescent Dyes); 0 (Sulfonic Acids); 0 (boron difluoride); 059QF0KO0R (Water); A3OGP4C37W (Cysteic Acid)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150609
[St] Status:MEDLINE
[do] DOI:10.1039/c5cc03649f


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[PMID]:25514859
[Au] Autor:Rouanne M; Perreaud A; Letang N; Yonneau L; Neuzillet Y; Hervé JM; Botto H; Lebret T
[Ad] Endereço:Department of Urology, Hôpital Foch, Suresnes, France; UFR des Sciences de la Santé, Université Versailles-Saint-Quentin-en-Yvelines, France. Electronic address: m.rouanne@hopital-foch.org.
[Ti] Título:Trends in renal function after radical cystectomy and ileal conduit diversion: new insights regarding estimated glomerular filtration rate variations.
[So] Source:Clin Genitourin Cancer;13(3):e139-44, 2015 Jun.
[Is] ISSN:1938-0682
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Our objectives were to evaluate the long-term renal function after radical cystectomy (RC) and ileal conduit diversion (ICD) and to analyze year-by-year the estimated glomerular filtration rate (eGFR) and morphologic upper urinary tract changes. PATIENTS AND METHODS: We retrospectively identified 226 patients who had undergone RC and ICD from 1980 to 2008, with regular postoperative follow-up visits. The eGFR was calculated using the Modification of Diet in Renal Disease equation at baseline and during follow-up. A decrease in renal function was defined as > 1 mL/min/1.73 m(2) annually. RESULTS: The median follow-up period after RC was 91 months (range, 61-235 months). The median eGFR decreased from 66 mL/min/1.73 m(2) (range, 17-139 mL/min/1.73 m(2)) to 59 mL/min/1.73 m(2) (range, 33-102 mL/min/1.73 m(2)). A rapid decline in renal function occurred during the first 2 postoperative years (-9 mL/min/1.73 m(2) and -4 mL/min/1.73 m(2) in the first and second year, respectively), with a moderate to slight decrease in the subsequent years. Urinary obstruction was diagnosed in 51 patients (23%). Among the patients who underwent prompt surgical treatment, we did not find any association with the eGFR decline (P = .8). CONCLUSION: Patients with urinary ICD have a lifelong risk of chronic kidney disease. Regular monitoring of renal function and the morphologic upper urinary tract will permit early diagnosis and treatment of modifiable factors, avoiding irreversible kidney damage.
[Mh] Termos MeSH primário: Rim/fisiopatologia
Insuficiência Renal Crônica/diagnóstico
Neoplasias da Bexiga Urinária/cirurgia
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Ácido Cisteico/efeitos adversos
Diagnóstico Precoce
Feminino
Taxa de Filtração Glomerular
Seres Humanos
Masculino
Meia-Idade
Insuficiência Renal Crônica/fisiopatologia
Estudos Retrospectivos
Neoplasias da Bexiga Urinária/fisiopatologia
Derivação Urinária/efeitos adversos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
A3OGP4C37W (Cysteic Acid)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150513
[Lr] Data última revisão:
150513
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141218
[St] Status:MEDLINE


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[PMID]:25180828
[Au] Autor:Ammann D; Becker R; Kohl A; Hänisch J; Nehls I
[Ad] Endereço:Federal Institute for Materials Research and Testing (BAM), Berlin, Germany.
[Ti] Título:Degradation of the ethyl glucuronide content in hair by hydrogen peroxide and a non-destructive assay for oxidative hair treatment using infra-red spectroscopy.
[So] Source:Forensic Sci Int;244:30-5, 2014 Nov.
[Is] ISSN:1872-6283
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:The assessment of quantification results of the alcohol abuse marker ethyl glucuronide (EtG) in hair in comparison to the cut-off values for the drinking behavior may be complicated by cosmetic hair bleaching. Thus, the impact of increasing exposure to hydrogen peroxide on the EtG content of hair was investigated. Simultaneously, the change of absorbance in the range of 1000-1100 cm(-1) indicative for the oxidation of cystine was investigated non-destructively by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) using pulverized portions of the respective hair samples. Hair samples treated with hydrogen peroxide consistently displayed a significantly increased absorbance at 1040 cm(-1) associated with the formation of cysteic acid. The EtG content decreased significantly if the hair was treated with alkaline hydrogen peroxide as during cosmetic bleaching. It could be shown that ATR-FTIR is capable of detecting an exposure to hydrogen peroxide when still no brightening was visible and already before the EtG content deteriorated significantly. Thus, hair samples suspected of having been exposed to oxidative treatment may be checked non-destructively by a readily available technique. This assay is also possible retrospectively after EtG extraction and using archived samples.
[Mh] Termos MeSH primário: Glucuronatos/análise
Cabelo/química
Peróxido de Hidrogênio/química
Oxidantes/química
[Mh] Termos MeSH secundário: Biomarcadores/análise
Ácido Cisteico
Toxicologia Forense
Seres Humanos
Masculino
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (Glucuronates); 0 (Oxidants); 17685-04-0 (ethyl glucuronide); A3OGP4C37W (Cysteic Acid); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140903
[St] Status:MEDLINE


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[PMID]:24916385
[Au] Autor:Daly R; Rogers S; Wandy J; Jankevics A; Burgess KE; Breitling R
[Ad] Endereço:School of Computing Science, University of Glasgow, Glasgow, Manchester Institute of Biotechnology, Faculty of Life Sciences, University of Manchester, Manchester and Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, UK.
[Ti] Título:MetAssign: probabilistic annotation of metabolites from LC-MS data using a Bayesian clustering approach.
[So] Source:Bioinformatics;30(19):2764-71, 2014 Oct.
[Is] ISSN:1367-4811
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:MOTIVATION: The use of liquid chromatography coupled to mass spectrometry has enabled the high-throughput profiling of the metabolite composition of biological samples. However, the large amount of data obtained can be difficult to analyse and often requires computational processing to understand which metabolites are present in a sample. This article looks at the dual problem of annotating peaks in a sample with a metabolite, together with putatively annotating whether a metabolite is present in the sample. The starting point of the approach is a Bayesian clustering of peaks into groups, each corresponding to putative adducts and isotopes of a single metabolite. RESULTS: The Bayesian modelling introduced here combines information from the mass-to-charge ratio, retention time and intensity of each peak, together with a model of the inter-peak dependency structure, to increase the accuracy of peak annotation. The results inherently contain a quantitative estimate of confidence in the peak annotations and allow an accurate trade-off between precision and recall. Extensive validation experiments using authentic chemical standards show that this system is able to produce more accurate putative identifications than other state-of-the-art systems, while at the same time giving a probabilistic measure of confidence in the annotations. AVAILABILITY AND IMPLEMENTATION: The software has been implemented as part of the mzMatch metabolomics analysis pipeline, which is available for download at http://mzmatch.sourceforge.net/.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Espectrometria de Massas/métodos
Metabolômica
[Mh] Termos MeSH secundário: Algoritmos
Teorema de Bayes
Análise por Conglomerados
Ácido Cisteico/análise
Interpretação Estatística de Dados
Distribuição Normal
Probabilidade
Reprodutibilidade dos Testes
Software
Triazóis/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Triazoles); A3OGP4C37W (Cysteic Acid)
[Em] Mês de entrada:1411
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140612
[St] Status:MEDLINE
[do] DOI:10.1093/bioinformatics/btu370


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[PMID]:24760173
[Au] Autor:Upadhyay LS; Verma N
[Ad] Endereço:Department of Biotechnology, National Institute of Technology, Raipur, Raipur, 492001, Chhattisgarh, India, contactlataupadhyay@gmail.com.
[Ti] Título:Synthesis and characterization of cysteine functionalized silver nanoparticles for biomolecule immobilization.
[So] Source:Bioprocess Biosyst Eng;37(11):2139-48, 2014 Nov.
[Is] ISSN:1615-7605
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A facile method for the aqueous phase synthesis of cysteine-functionalized silver nanoparticles by potato extract has been reported in the present work. These functionalized nanoparticles were then used for the covalent immobilization of a biomolecule, alkaline phosphatase, on its surface through carbodiimide coupling. Different reaction parameters such as cysteine concentration, reducing agent concentration, temperature, pH and reaction time were varied during the nanoparticles' formation, and their effects on plasmon resonance were studied using Ultraviolet-visible spectroscopy. Fourier transform infrared spectroscopy was used to confirm the surface modification of silver nanoparticles by cysteine and the particle size analysis was done using particle size analyzer, which showed the average nanoparticles' size of 61 nm for bare silver nanoparticles and 201 nm for the enzyme-immobilized nanoparticles. The synthesized nanoparticles were found to be highly efficient for the covalent immobilization of alkaline phosphatase on its surface and retained 67% of its initial enzyme activity (9.44 U/mg), with 75% binding efficiency. The shelf life of the enzyme-nanoparticle bioconjugates was found to be 60 days, with a 12% loss in the initial enzyme activity. With a simple synthesis strategy, high immobilization efficiency and enhanced stability, these enzyme-coated nanoparticles have the potential for further integration into the biosensor technology.
[Mh] Termos MeSH primário: Ácido Cisteico/química
Enzimas Imobilizadas
Nanopartículas Metálicas/química
Prata/química
[Mh] Termos MeSH secundário: Fosfatase Alcalina
Técnicas Biossensoriais/métodos
Estabilidade Enzimática
Concentração de Íons de Hidrogênio
Nanopartículas Metálicas/ultraestrutura
Microscopia Eletrônica de Varredura
Nanotecnologia
Tamanho da Partícula
Espectrofotometria Ultravioleta
Espectroscopia de Infravermelho com Transformada de Fourier
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzymes, Immobilized); 3M4G523W1G (Silver); A3OGP4C37W (Cysteic Acid); EC 3.1.3.1 (Alkaline Phosphatase)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:141010
[Lr] Data última revisão:
141010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140425
[St] Status:MEDLINE
[do] DOI:10.1007/s00449-014-1191-8


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[PMID]:23597911
[Au] Autor:Liu W; Zhang J; Li C; Tang L; Zhang Z; Yang M
[Ad] Endereço:College of Chemistry, Jilin University, Changchun 130012, PR China.
[Ti] Título:A novel composite film derived from cysteic acid and PDDA-functionalized graphene: enhanced sensing material for electrochemical determination of metronidazole.
[So] Source:Talanta;104:204-11, 2013 Jan 30.
[Is] ISSN:1873-3573
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel composite film derived from cysteic acid and poly(diallydimethylammonium chloride)-functionalized graphene (PDDA-GN) was employed as an enhanced electrode material for ultrasensitive determination of metronidazole. The cysteic acid/PDDA-GN composite film was prepared by the electrochemical grafting of cysteic acid onto the PDDA-GN coated glassy carbon electrode (GCE). The cyclic voltammetry investigations reveal that the peak current of metronidazole reduction at the cysteic acid/PDDA-GN/GCE was remarkably enhanced compared to the bare GCE, the cysteic acid/GCE and the PDDA-GN/GCE. This result implies the synergistic electrocatalytic effect of cysteic acid and PDDA-GN. The fabricated sensor shows linear response to metronidazole in the ranges of 10 nM-1 µM and 70 µM-800 µM, with a detection limit of 2.3 nM (S/N=3). The heterogeneous electron transfer rate constant and the diffusion coefficient of metronidazole were further evaluated by rotating disk electrode experiments. Moreover, we applied the present method to the determination of metronidazole in urine and lake water with satisfactory results.
[Mh] Termos MeSH primário: Anti-Infecciosos/análise
Ácido Cisteico/química
Grafite/química
Metronidazol/análise
Polietilenos/química
Compostos de Amônio Quaternário/química
[Mh] Termos MeSH secundário: Anti-Infecciosos/química
Técnicas Eletroquímicas
Seres Humanos
Lagos/química
Metronidazol/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Polyethylenes); 0 (Quaternary Ammonium Compounds); 140QMO216E (Metronidazole); 26062-79-3 (poly-N,N-dimethyl-N,N-diallylammonium chloride); 7782-42-5 (Graphite); A3OGP4C37W (Cysteic Acid)
[Em] Mês de entrada:1311
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130420
[St] Status:MEDLINE


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[PMID]:23044173
[Au] Autor:Zhang F; Gu S; Ding Y; Li L; Liu X
[Ad] Endereço:School of Materials Science and Engineering, Shanghai University, Shanghai 200444, PR China.
[Ti] Título:Simultaneous determination of ofloxacin and gatifloxacin on cysteic acid modified electrode in the presence of sodium dodecyl benzene sulfonate.
[So] Source:Bioelectrochemistry;89:42-9, 2013 Feb.
[Is] ISSN:1878-562X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A novel cysteic acid modified carbon paste electrode (cysteic acid/CPE) based on electrochemical oxidation of L-cysteine was developed to simultaneously determine ofloxacin and gatifloxacin in the presence of sodium dodecyl benzene sulfonate (SDBS). Fourier transform infrared spectra (FTIR) indicated that L-cysteine was oxidated to cysteic acid. Electrochemical impedance spectroscopy (EIS) and cyclic voltammograms (CV) indicated that cysteic acid was successfully modified on electrode. The large peak separation (116 mV) between ofloxacin and gatifloxacin was obtained on cysteic acid/CPE while only one oxidation peak was found on bare electrode. And the peak currents increased 5 times compared to bare electrode. Moreover, the current could be further enhanced in the presence of an anionic surfactant, sodium dodecyl benzene sulfonate. The differential pulse voltammograms (DPV) exhibited that the oxidation peak currents were linearly proportional to their concentrations in the range of 0.06-10 µM for ofloxacin and 0.02-200 µM for gatifloxacin, and the detection limits of ofloxacin and gatifloxacin were 0.02 µM and 0.01 µM (S/N=3), respectively. This proposed method was successfully applied to determine ofloxacin and gatifloxacin in pharmaceutical formulations and human serum samples.
[Mh] Termos MeSH primário: Benzenossulfonatos/química
Ácido Cisteico/química
Eletroquímica/métodos
Fluoroquinolonas/análise
Fluoroquinolonas/química
Ofloxacino/análise
Ofloxacino/química
[Mh] Termos MeSH secundário: Carbono/química
Química Farmacêutica
Eletroquímica/instrumentação
Eletrodos
Fluoroquinolonas/sangue
Seres Humanos
Ofloxacino/sangue
Oxirredução
Propriedades de Superfície
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Benzenesulfonates); 0 (Fluoroquinolones); 60NSK897G9 (dodecylbenzenesulfonic acid); 7440-44-0 (Carbon); A3OGP4C37W (Cysteic Acid); A4P49JAZ9H (Ofloxacin); L4618BD7KJ (gatifloxacin)
[Em] Mês de entrada:1306
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121010
[St] Status:MEDLINE


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[PMID]:23038267
[Au] Autor:Liu P; Ge X; Ding H; Jiang H; Christensen BM; Li J
[Ad] Endereço:Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA.
[Ti] Título:Role of glutamate decarboxylase-like protein 1 (GADL1) in taurine biosynthesis.
[So] Source:J Biol Chem;287(49):40898-906, 2012 Nov 30.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This manuscript concerns the tissue-specific transcription of mouse and cattle glutamate decarboxylase-like protein 1 (GADL1) and the biochemical activities of human GADL1 recombinant protein. Bioinformatic analysis suggested that GADL1 appears late in evolution, only being found in reptiles, birds, and mammals. RT-PCR determined that GADL1 mRNA is transcribed at high levels in mouse and cattle skeletal muscles and also in mouse kidneys. Substrate screening determined that GADL1, unlike its name implies, has no detectable GAD activity, but it is able to efficiently catalyze decarboxylation of aspartate, cysteine sulfinic acid, and cysteic acid to ß-alanine, hypotaurine, and taurine, respectively. Western blot analysis verified the presence of GADL1 in mouse muscles, kidneys, C2C12 myoblasts, and C2C12 myotubes. Incubation of the supernatant of fresh muscle or kidney extracts with cysteine sulfinic acid resulted in the detection of hypotaurine or taurine in the reaction mixtures, suggesting the possible involvement of GADL1 in taurine biosynthesis. However, when the tissue samples were incubated with aspartate, no ß-alanine production was observed. We proposed several possibilities that might explain the inactivation of ADC activity of GADL1 in tissue protein extracts. Although ß-alanine-producing activity was not detected in the supernatant of tissue protein extracts, its potential role in ß-alanine synthesis cannot be excluded. There are several inhibitors of the ADC activity of GADL1 identified. The discovery of GADL1 biochemical activities, in conjunction with its expression and activities in muscles and kidneys, provides some tangible insight toward establishing its physiological function(s).
[Mh] Termos MeSH primário: Carboxiliases/fisiologia
Glutamato Descarboxilase/metabolismo
Taurina/biossíntese
[Mh] Termos MeSH secundário: Animais
Carboxiliases/genética
Carboxiliases/metabolismo
Linhagem Celular
Ácido Cisteico/metabolismo
Cisteína/análogos & derivados
Cisteína/metabolismo
Rim/metabolismo
Cinética
Camundongos
Modelos Biológicos
Músculos/metabolismo
Mioblastos/metabolismo
Proteínas Recombinantes/metabolismo
Especificidade por Substrato
Taurina/análogos & derivados
Taurina/metabolismo
Distribuição Tecidual
beta-Alanina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Recombinant Proteins); 11P2JDE17B (beta-Alanine); 1EQV5MLY3D (Taurine); 2381-08-0 (cysteine sulfinic acid); 5L08GE4332 (hypotaurine); A3OGP4C37W (Cysteic Acid); EC 4.1.1.- (Carboxy-Lyases); EC 4.1.1.- (GADL1 protein, mouse); EC 4.1.1.15 (Glutamate Decarboxylase); EC 4.1.1.15 (glutamate decarboxylase 1); K848JZ4886 (Cysteine)
[Em] Mês de entrada:1302
[Cu] Atualização por classe:161202
[Lr] Data última revisão:
161202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121006
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M112.393728



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