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[PMID]:28470871
[Au] Autor:Misan A; Chan WY; Trott D; Hill PB
[Ad] Endereço:School of Animal and Veterinary Sciences, University of Adelaide, Roseworthy, South Australia, 5371, Australia.
[Ti] Título:Survival of Staphylococcus pseudintermedius in modified Romanowsky staining solutions.
[So] Source:Vet Dermatol;28(4):333-e71, 2017 Aug.
[Is] ISSN:1365-3164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Stains that are used regularly for patient-side diagnosis to rapidly identify bacterial and fungal infections could become contaminated by common pathogens, such as Staphylococcus pseudintermedius, during slide immersion. HYPOTHESIS/OBJECTIVES: To determine whether the inoculation of S. pseudintermedius into modified Romanowsky type stains (Quick Dip ) results in viable bacterial contamination and whether this is influenced by the addition of organic debris (canine hair and skin). METHODS: A clinical isolate of S. pseudintermedius was inoculated into clean and organically contaminated Quick Dip solutions (methanol fixative, eosin, methylene blue), and positive (broth) and negative (bleach) controls. Each solution was tested for the presence of viable bacteria by counting the number of colony forming units (CFU/mL) at various time points. Solutions also were examined under high power microscopy to count the number of visible bacteria at each time point. RESULTS: Staphylococcus pseudintermedius was able to survive in the clean and contaminated Quick Dip stains for at least one hour, but by 24 h no viable bacteria remained. Survival of the bacteria was not supported in the fixative at any time point. Staphylococcus pseudintermedius remained visible under high power microscopy for up to 2 weeks in all organically contaminated solutions of the Quick Dip set. CONCLUSIONS AND CLINICAL IMPORTANCE: Staphylococcus pseudintermedius only remains viable in eosin and methylene blue for short periods of time, but the prolonged visibility of dead organisms could theoretically lead to the misdiagnosis of cytology samples.
[Mh] Termos MeSH primário: Corantes Azur/metabolismo
Amarelo de Eosina-(YS)/metabolismo
Contaminação de Equipamentos
Staphylococcus/fisiologia
[Mh] Termos MeSH secundário: Animais
Carga Bacteriana
Cães
Cabelo/microbiologia
Microscopia/veterinária
Pele/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azure Stains); 0 (Romanowsky-Giemsa stain); TDQ283MPCW (Eosine Yellowish-(YS))
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171221
[Lr] Data última revisão:
171221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1111/vde.12435


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[PMID]:28508922
[Au] Autor:Arias MH; Deharo E; Valentin A; Garavito G
[Ad] Endereço:Universidad Nacional de Colombia, Sede Bogotá, Facultad de Ciencias, Departamento de Farmacia (DFUNC), Grupo de Investigación FaMeTra (Farmacología de la Medicina Tradicional y Popular), Carrera 30 45-03, Bogotá D.C., 111311, Colombia.
[Ti] Título:Adaptation and optimization of a fluorescence-based assay for in vivo antimalarial drug screening.
[So] Source:Parasitol Res;116(7):1955-1962, 2017 Jul.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The in vivo efficacy of potential antimalarials is usually evaluated by direct microscopic determination of the parasitaemia of Plasmodium-infected mice on Giemsa-stained blood smears. This process is time-consuming, requires experienced technicians and is not automatable. Therefore, we optimized a SYBR Green I (SYBRG I) fluorescence-based assay to fluorometers commonly available in many research laboratories. This technique was originally developed to assess parasitaemia in humans by cytometry. We defined optimal conditions with Plasmodium berghei-infected mice, standard lysis buffer (Tris, EDTA, saponin and Triton), whole blood cells and 2 h staining incubation with SYBRG I 2X. The fluorescence background generated by uninfected whole blood cells was low (around 4.6%), and the linearity high (r  = 0.96), with parasitaemia ranging from 1.4 to 60%. The Bland-Altman plot showed a strong correlation between SYBRG I and Giemsa gold standard method; Z'-factor was >0.5. These findings suggest that our fluorescence-based assay is suitable for in vivo antimalarial drug assessment in a malaria murine model. It can help to overcome the human bias found with microscopic techniques.
[Mh] Termos MeSH primário: Antimaláricos/uso terapêutico
Malária/tratamento farmacológico
Testes de Sensibilidade Microbiana/métodos
Plasmodium berghei/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Corantes Azur
Fluorescência
Masculino
Camundongos
Compostos Orgânicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antimalarials); 0 (Azure Stains); 0 (Organic Chemicals); 163795-75-3 (SYBR Green I)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-017-5477-z


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[PMID]:28403153
[Au] Autor:Karakus M; Nasereddin A; Onay H; Karaca E; Özkeklikçi A; Jaffe CL; Kuhls K; Özbilgin A; Ertabaklar H; Demir S; Özbel Y; Töz S
[Ad] Endereço:Ege University, Faculty of Medicine, Department of Parasitology, Izmir, Turkey.
[Ti] Título:Epidemiological analysis of Leishmania tropica strains and giemsa-stained smears from Syrian and Turkish leishmaniasis patients using multilocus microsatellite typing (MLMT).
[So] Source:PLoS Negl Trop Dis;11(4):e0005538, 2017 Apr.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Turkey is located in an important geographical location, in terms of the epidemiology of vector-borne diseases, linking Asia and Europe. Cutaneous leishmaniasis (CL) is one of the endemic diseases in a Turkey and according to the Ministry Health of Turkey, 45% of CL patients originate from Sanliurfa province located in southeastern Turkey. Herein, the epidemiological status of CL, caused by L. tropica, in Turkey was examined using multilocus microsatellite typing (MLMT) of strains obtained from Turkish and Syrian patients. A total of 38 cryopreserved strains and 20 Giemsa-stained smears were included in the present study. MLMT was performed using 12 highly specific microsatellite markers. Delta K (ΔK) calculation and Bayesian statistics were used to determine the population structure. Three main populations (POP A, B and C) were identified and further examination revealed the presence of three subpopulations for POP B and C. Combined analysis was performed using the data of previously typed L. tropica strains and Mediterranean and Sanliurfa populations were identified. This finding suggests that the epidemiological status of L. tropica is more complicated than expected when compared to previous studies. A new population, comprised of Syrian L. tropica samples, was reported for the first time in Turkey, and the data presented here will provide new epidemiological information for further studies.
[Mh] Termos MeSH primário: Leishmania tropica/isolamento & purificação
Leishmaniose Cutânea/epidemiologia
Repetições de Microssatélites
[Mh] Termos MeSH secundário: Corantes Azur
Teorema de Bayes
DNA de Protozoário/isolamento & purificação
Variação Genética
Mapeamento Geográfico
Seres Humanos
Leishmania tropica/classificação
Tipagem de Sequências Multilocus
Filogenia
Síria/epidemiologia
Turquia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azure Stains); 0 (DNA, Protozoan)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005538


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[PMID]:28403073
[Au] Autor:Lilyquist J; White KA; Lee RJ; Philips GK; Hughes CR; Torres SM
[Ad] Endereço:aDepartment of Internal Medicine, Division of Epidemiology, University of New Mexico, Albuquerque, NM bDepartment of Health Sciences Research, Mayo Clinic, Rochester, MN cCancer Research and Treatment Center dCenter for HPV Prevention, Department of Pathology, University of New Mexico, Albuquerque, NM.
[Ti] Título:Quantitative Analysis of Immunohistochemistry in Melanoma Tumors.
[So] Source:Medicine (Baltimore);96(15):e6432, 2017 Apr.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Identification of positive staining is often qualitative and subjective. This is particularly troublesome in pigmented melanoma lesions, because melanin is difficult to distinguish from the brown stain resulting from immunohistochemistry (IHC) using horse radish peroxidase developed with 3,3'-Diaminobenzidine (HRP-DAB). We sought to identify and quantify positive staining, particularly in melanoma lesions. We visualized G-protein coupled estrogen receptor (GPER) expression developed with HRP-DAB and counterstained with Azure B (stains melanin) in melanoma tissue sections (n = 3). Matched sections (n = 3), along with 22 unmatched sections, were stained only with Azure B as a control. Breast tissue (n = 1) was used as a positive HRP-DAB control. Images of the stained tissues were generated using a Nuance Spectral Imaging Camera. Analysis of the images was performed using the Nuance Spectral Imaging software and SlideBook. Data was analyzed using a Kruskal-Wallis one way analysis of variance (ANOVA). We showed that a pigmented melanoma tissue doubly stained with anti-GPER HRP-DAB and Azure B can be unmixed using spectra derived from a matched, Azure B-only section, and an anti-GPER HRP-DAB control. We unmixed each of the melanoma lesions using each of the Azure B spectra, evaluated the mean intensity of positive staining, and examined the distribution of the mean intensities (P = .73; Kruskal-Wallis). These results suggest that this method does not require a matched Azure B-only stained control tissue for every melanoma lesion, allowing precious tissues to be conserved for other studies. Importantly, this quantification method reduces the subjectivity of protein expression analysis, and provides a valuable tool for accurate evaluation, particularly for pigmented tissues.
[Mh] Termos MeSH primário: Imuno-Histoquímica/métodos
Melanoma/patologia
Receptores Acoplados a Proteínas-G/metabolismo
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: 3,3'-Diaminobenzidina
Análise de Variância
Corantes Azur
Biomarcadores Tumorais/metabolismo
Peroxidase do Rábano Silvestre
Seres Humanos
Melaninas/metabolismo
Melanoma/metabolismo
Pigmentação
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azure Stains); 0 (Biomarkers, Tumor); 0 (Melanins); 0 (Receptors, G-Protein-Coupled); 2RV4T6KHQI (3,3'-Diaminobenzidine); 7C8BEL8WQV (Azure B); EC 1.11.1.- (Horseradish Peroxidase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000006432


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[PMID]:28372570
[Au] Autor:Parsel SM; Gustafson SA; Friedlander E; Shnyra AA; Adegbulu AJ; Liu Y; Parrish NM; Jamal SA; Lofthus E; Ayuk L; Awasom C; Henry CJ; McArthur CP
[Ad] Endereço:Department of Pathology, Kansas City University of Medicine and Biosciences, 1750 Independence Ave, Kansas, MO, 64106, USA. sparsel@me.com.
[Ti] Título:Malaria over-diagnosis in Cameroon: diagnostic accuracy of Fluorescence and Staining Technologies (FAST) Malaria Stain and LED microscopy versus Giemsa and bright field microscopy validated by polymerase chain reaction.
[So] Source:Infect Dis Poverty;6(1):32, 2017 Apr 04.
[Is] ISSN:2049-9957
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Malaria is a major world health issue and its continued burden is due, in part, to difficulties in the diagnosis of the illness. The World Health Organization recommends confirmatory testing using microscopy-based techniques or rapid diagnostic tests (RDT) for all cases of suspected malaria. In regions where Plasmodium species are indigenous, there are multiple etiologies of fever leading to misdiagnoses, especially in populations where HIV is prevalent and children. To determine the frequency of malaria infection in febrile patients over an 8-month period at the Regional Hospital in Bamenda, Cameroon, we evaluated the clinical efficacy of the Flourescence and Staining Technology (FAST) Malaria stain and ParaLens Advance microscopy system (FM) and compared it with conventional bright field microscopy and Giemsa stain (GS). METHODS: Peripheral blood samples from 522 patients with a clinical diagnosis of "suspected malaria" were evaluated using GS and FM methods. A nested PCR assay was the gold standard to compare the two methods. PCR positivity, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were determined. RESULTS: Four hundred ninety nine samples were included in the final analysis. Of these, 30 were positive via PCR (6.01%) with a mean PPV of 19.62% and 27.99% for GS and FM, respectively. The mean NPV was 95.01% and 95.28% for GS and FM, respectively. Sensitivity was 26.67% in both groups and specificity was 92.78% and 96.21% for GS and FM, respectively. An increased level of diagnostic discrepancy was observed between technicians based upon skill level using GS, which was not seen with FM. CONCLUSIONS: The frequency of malarial infections confirmed via PCR among patients presenting with fever and other symptoms of malaria was dramatically lower than that anticipated based upon physicians' clinical suspicions. A correlation between technician skill and accuracy of malaria diagnosis using GS was observed that was less pronounced using FM. Additionally, FM increased the specificity and improved the PPV, suggesting this relatively low cost approach could be useful in resource-limited environments. Anecdotally, physicians were reluctant to not treat all patients symptomatically before results were known and in spite of a negative microscopic diagnosis, highlighting the need for further physician education to avoid this practice of overtreatment. A larger study in an area with a known high prevalence is being planned to compare the two microscopy methods against available RDTs.
[Mh] Termos MeSH primário: Malária/diagnóstico
Microscopia/métodos
Reação em Cadeia da Polimerase/métodos
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Corantes Azur
Camarões
Criança
Pré-Escolar
Corantes
Feminino
Seres Humanos
Lactente
Malária/sangue
Malária/parasitologia
Masculino
Meia-Idade
Prevalência
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azure Stains); 0 (Coloring Agents)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE
[do] DOI:10.1186/s40249-017-0251-0


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[PMID]:28324880
[Au] Autor:Zhao Z; Paquette C; Shah AA; Atkins KA; Frierson HF
[Ad] Endereço:Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
[Ti] Título:Fine Needle Aspiration Cytology of Diffuse-Type Tenosynovial Giant Cell Tumors.
[So] Source:Acta Cytol;61(2):160-164, 2017.
[Is] ISSN:1938-2650
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Tenosynovial giant cell tumor (TSGCT), also known as giant cell tumor of tendon sheath or pigmented villonodular synovitis, is the most common benign tumor of the tendon and synovium. The intra-articular diffuse type can present as a large infiltrative mass involving adjacent soft tissue and sometimes causes secondary destruction of bone, which leads to radiographic and clinical concern for malignancy. The tumor may also be purely extra-articular. CASE: Here, we report the fine needle aspiration cytology findings of 2 cases of diffuse-type TSGCT with large mononuclear cells with eccentric nuclei, finely granular cytoplasm, and a peripheral well-defined cytoplasmic rim of hemosiderin ("ladybird cells"). CONCLUSION: Although the presence of ladybird cells has been described in tissue sections of TSGCT, their identification in cytological specimens has not been reported to our knowledge. When observed, their presence may aid in differentiating TSGCT from other lesions with multinucleated osteoclast-type giant cells occurring at or near joints.
[Mh] Termos MeSH primário: Biópsia por Agulha Fina
Tumor de Células Gigantes de Bainha Tendinosa/diagnóstico
Células Gigantes/patologia
[Mh] Termos MeSH secundário: Idoso
Corantes Azur
Biomarcadores Tumorais/análise
Núcleo Celular/patologia
Diagnóstico Diferencial
Feminino
Tumor de Células Gigantes de Bainha Tendinosa/química
Tumor de Células Gigantes de Bainha Tendinosa/patologia
Células Gigantes/química
Hemossiderina/análise
Seres Humanos
Imagem por Ressonância Magnética
Masculino
Azul de Metileno
Teste de Papanicolaou
Valor Preditivo dos Testes
Tomografia Computadorizada por Raios X
Xantenos
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azure Stains); 0 (Biomarkers, Tumor); 0 (Diff Quik); 0 (Xanthenes); 9011-92-1 (Hemosiderin); T42P99266K (Methylene Blue)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170530
[Lr] Data última revisão:
170530
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1159/000457828


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[PMID]:28301305
[Au] Autor:Beldi N; Mansouri R; Bettaieb J; Yaacoub A; Souguir Omrani H; Saadi Ben Aoun Y; Saadni F; Guizani I; Guerbouj S
[Ad] Endereço:1 Laboratoire d'Epidémiologie Moléculaire et Pathologie Expérimentale Appliquée aux Maladies Infectieuses (LR11IPT04), Institut Pasteur de Tunis, Université Tunis El Manar , Tunis, Tunisia .
[Ti] Título:Molecular Characterization of Leishmania Parasites in Giemsa-Stained Slides from Cases of Human Cutaneous and Visceral Leishmaniasis, Eastern Algeria.
[So] Source:Vector Borne Zoonotic Dis;17(6):416-424, 2017 Jun.
[Is] ISSN:1557-7759
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In Algeria, visceral leishmaniasis (VL) is due to Leishmania (L.) infantum, while three cutaneous forms (CL) are caused by Leishmania major, Leishmania tropica and Leishmania infantum. In this study, the use of Giemsa-stained slides was evaluated with two PCR techniques, in Eastern Algeria. MATERIALS AND METHODS: A total of 136 samples corresponding to 100 CL smears (skin scrapings) and 36 VL slides (bone marrow aspirates) collected from 2008 to 2014 were tested. Upon DNA extraction, two PCRs were used to amplify the ribosomal Internal Transcribed Spacer 1 (ITS1) and mini-exon genes. Amplified products were digested (PCR-RFLP) and profiles analyzed for Leishmania species identification. A statistical analysis was also performed. RESULTS: ITS1-PCR was found significantly more sensitive than mini-exon-PCR (77.95% positives vs. 67.65%; p = 0.001). Comparison of PCR positivity showed statistically significant differences between old and recently prepared slides suggesting a better use of recent slides in PCR analyses. For species identification, PCR-restriction fragment length polymorphism (RFLP) results of ITS1 and mini-exon were concordant. L. infantum was identified from VL cases and L. infantum, L. major, and L. tropica from CL ones. According to geographical origin, L. infantum was found in North-Eastern provinces, while L. major was distributed from the North to the Center-East of Algeria. Interestingly, two L. tropica samples were identified in Annaba, located far North-East Algeria. CONCLUSION: Distribution of leishmaniasis in Eastern parts of Algeria, besides finding of L. tropica in the far North, is in this study described for the first time using molecular tools, thus confirming the usefulness of slides for PCR identification of Leishmania parasites in retrospective epidemiological investigations.
[Mh] Termos MeSH primário: Leishmania/isolamento & purificação
Leishmaniose Cutânea/parasitologia
Leishmaniose Visceral/parasitologia
[Mh] Termos MeSH secundário: Argélia/epidemiologia
Animais
Corantes Azur
Medula Óssea/parasitologia
DNA Espaçador Ribossômico/genética
Seres Humanos
Leishmania/classificação
Leishmania/genética
Leishmaniose Cutânea/epidemiologia
Leishmaniose Visceral/epidemiologia
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Pele/parasitologia
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azure Stains); 0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1089/vbz.2016.2071


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[PMID]:28272845
[Au] Autor:Baum JE; Zhang P; Hoda RS; Geraghty B; Rennert H; Narula N; Fernandes HD
[Ad] Endereço:Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York.
[Ti] Título:Accuracy of next-generation sequencing for the identification of clinically relevant variants in cytology smears in lung adenocarcinoma.
[So] Source:Cancer;125(6):398-406, 2017 Jun.
[Is] ISSN:1097-0142
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Minimally invasive diagnostic procedures such as needle-core biopsy and fine-needle aspiration provide adequate material for molecular analyses. Advances in precision oncology are trending toward the interrogation of limited amounts of genomic material to guide clinical and therapeutic decisions. The aim of this study was to investigate the minimum cellularity needed on cytologic smears for the identification of clinically relevant variants with next-generation sequencing (NGS). METHODS: Thirty cases of cytologically diagnosed, resection-proven primary lung adenocarcinoma were identified. Nineteen of the 30 cases were known to harbor actionable variants. One Diff-Quik (DQ)-stained slide and 1 Papanicolaou (Pap)-stained slide were selected from each case. Cases were categorized as containing fewer than 100 tumor cells, 100 to 500 tumor cells, or more than 500 tumor cells. NGS was performed on the Ion Torrent platform. RESULTS: NGS was successfully performed on all cell blocks and on 90% of the smears. Paired DQ and Pap smears showed similar cellularity, and cases that differed in cellularity were within 1 category of each other. The cases with more than 100 tumor cells had a 93% success rate; this was significantly different from the situation for cases with fewer than 100 tumor cells, which were successfully sequenced only 67% of the time. Overall, NGS was able to provide clinically relevant information for 83% of DQ smears and for 90% of Pap smears tested. CONCLUSIONS: The data show a significantly higher likelihood of successful NGS with cytologic smears with more than 100 tumor cells. There was a trend for a higher NGS success rate with Pap smears versus DQ smears. Cancer Cytopathol 2017;125:398-406. © 2017 American Cancer Society.
[Mh] Termos MeSH primário: Adenocarcinoma/genética
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Neoplasias Pulmonares/genética
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Adenocarcinoma/patologia
Proteína da Polipose Adenomatosa do Colo/genética
Idoso
Idoso de 80 Anos ou mais
Corantes Azur
Biópsia por Agulha Fina
Inibidor de Quinase Dependente de Ciclina p18/genética
Técnicas Citológicas
Feminino
Seres Humanos
Neoplasias Pulmonares/patologia
Masculino
Azul de Metileno
Meia-Idade
Estadiamento de Neoplasias
Teste de Papanicolaou
Proteínas Serina-Treonina Quinases/genética
Proteínas Proto-Oncogênicas B-raf/genética
Proteínas Proto-Oncogênicas p21(ras)/genética
Receptor do Fator de Crescimento Epidérmico/genética
Proteína Supressora de Tumor p53/genética
Xantenos
beta Catenina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (APC protein, human); 0 (Adenomatous Polyposis Coli Protein); 0 (Azure Stains); 0 (CDKN2A protein, human); 0 (CTNNB1 protein, human); 0 (Cyclin-Dependent Kinase Inhibitor p18); 0 (Diff Quik); 0 (KRAS protein, human); 0 (TP53 protein, human); 0 (Tumor Suppressor Protein p53); 0 (Xanthenes); 0 (beta Catenin); EC 2.7.1.- (STK11 protein, human); EC 2.7.10.1 (EGFR protein, human); EC 2.7.10.1 (Receptor, Epidermal Growth Factor); EC 2.7.11.1 (BRAF protein, human); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins B-raf); EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras)); T42P99266K (Methylene Blue)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE
[do] DOI:10.1002/cncy.21844


  9 / 1457 MEDLINE  
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[PMID]:28222637
[Au] Autor:Eksi F; Özgöztasi O; Karsligil T; Saglam M
[Ad] Endereço:1 Department of Medical Microbiology, Gaziantep University, Gaziantep, Turkey.
[Ti] Título:Genotyping Leishmania promastigotes isolated from patients with cutaneous leishmaniasis in south-eastern Turkey.
[So] Source:J Int Med Res;45(1):114-122, 2017 Feb.
[Is] ISSN:1473-2300
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objective Cutaneous leishmaniasis (CL) is a significant disease in south-eastern Anatolia because it is prevalent among Syrian refugees. We identified the causative Leishmania species in CL patients using molecular methods. Methods Novy-MacNeal-Nicolle medium was inoculated with aspirated fluid from suspected CL lesions and tested for amastigotes with Giemsa staining. PCR amplified the internal transcribed spacer 1 (ITS1) of the Leishmania genome in cultures containing Leishmania promastigotes from 100 patients, which were genotyped with a restriction fragment length polymorphism (RFLP) analysis. A phylogenetic tree was constructed from ITS1 sequences of 95 culture fluid samples from these patients. Results Leishmania amastigotes were detected in 92% of cultures with growth. Leishmania promastigotes were typed as Leishmania tropica with both PCR-RFLP and sequencing. Conclusions Identification of L. tropica as the causative agent of CL in our region allows the clinical course to be predicted, and guides treatment decisions and preventive measures.
[Mh] Termos MeSH primário: DNA de Protozoário/genética
Leishmania tropica/genética
Leishmaniose Cutânea/diagnóstico
Estágios do Ciclo de Vida/genética
Filogenia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Corantes Azur
DNA Intergênico/genética
Feminino
Genótipo
Seres Humanos
Leishmania tropica/classificação
Leishmania tropica/crescimento & desenvolvimento
Leishmania tropica/isolamento & purificação
Leishmaniose Cutânea/epidemiologia
Leishmaniose Cutânea/parasitologia
Leishmaniose Cutânea/patologia
Masculino
Tipagem Molecular
Polimorfismo de Fragmento de Restrição
Refugiados
Análise de Sequência de DNA
Pele/parasitologia
Pele/patologia
Turquia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azure Stains); 0 (DNA, Intergenic); 0 (DNA, Protozoan)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE
[do] DOI:10.1177/0300060516677155


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[PMID]:28098484
[Au] Autor:Bezrukov AV
[Ad] Endereço:a Electromechanical Company EMCO LLC , Moscow , Russia.
[Ti] Título:Romanowsky staining, the Romanowsky effect and thoughts on the question of scientific priority.
[So] Source:Biotech Histochem;92(1):29-35, 2017.
[Is] ISSN:1473-7760
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:I give an historical account and analysis of the scientific priority of the discovery of the polychrome staining of microscopic biological preparations provided by mixtures of eosin plus methylene blue and its derivatives, especially azure B. I maintain that both the formal priority for the discovery of the polychrome staining phenomenon and credit for initiating the development of a technique of polychrome staining properly belong to D. L. Romanowsky. His scientific work demonstrated the possibility of using a simple technique to stain hematological preparations selectively to give good contrast, high resolution and the ability to identify malaria parasites. Romanowsky's approach constituted the starting point for the development of a family of polychrome stains for microscopic investigation of hematological preparations by a number of his contemporaries.
[Mh] Termos MeSH primário: Corantes Azur/história
Amarelo de Eosina-(YS)/química
Azul de Metileno/química
Coloração e Rotulagem/história
Coloração e Rotulagem/métodos
[Mh] Termos MeSH secundário: Corantes/química
Amarelo de Eosina-(YS)/história
História do Século XIX
Seres Humanos
Malária/diagnóstico
Malária/parasitologia
Plasmodium/citologia
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Azure Stains); 0 (Coloring Agents); 0 (Romanowsky-Giemsa stain); T42P99266K (Methylene Blue); TDQ283MPCW (Eosine Yellowish-(YS))
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170323
[Lr] Data última revisão:
170323
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170119
[St] Status:MEDLINE
[do] DOI:10.1080/10520295.2016.1250285



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