Base de dados : MEDLINE
Pesquisa : D02.886.369.869 [Categoria DeCS]
Referências encontradas : 1562 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 157 ir para página                         

  1 / 1562 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28649006
[Au] Autor:Liang H; Xu J; Liu Y; Zhang J; Peng W; Yan J; Li Z; Li Q
[Ad] Endereço:Collaborative Innovation Center of Yangtze River Region Green Pharmaceuticals, Zhejiang University of Technology, Hangzhou, China; College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, China.
[Ti] Título:Optimization of hydrogel containing toluidine blue O for photodynamic therapy by response surface methodology.
[So] Source:J Photochem Photobiol B;173:389-396, 2017 Aug.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Photodynamic therapy with toluidine blue O (TBO) hydrogel exhibits antibacterial activity against Staphylococcus aureus and Escherichia coli in this paper. The response surface methodology is employed to optimize formulations for antibacterial activity. The optimal formulations are carbomer concentration of 3% (w/v), TBO concentration of 0.1mg/mL and the quality ratio of NaOH and carbomer of 0.4 (w/w). Under the optimized formulations, the log-transformed of CFUmL on the Staphylococcus aureus and Escherichia coli are 0.84 and 1.26 (the log-transformed of CFUmL of negative control groups on the Staphylococcus aureus and Escherichia coli are 8.21 and 8.47), respectively. In comparison with photodynamic therapy with TBO aqueous solution, the proposed formulations provide a much stronger antibacterial activity against Staphylococcus aureus and Escherichia coli. TBO hydrogels are stable during 6weeks at three different temperatures (4, 25 and 40°C) with respect to no change of color, transparency, pH and viscosity. 50% and 68.26% of TBO are released from carbomer hydrogel after 4h and 24h, respectively. TBO hydrogel alone or light alone (630nm) treatment is incapable of showing antibacterial activity against Staphylococcus aureus and Escherichia coli. Therefore, photodynamic therapy with the novel optimized TBO hydrogel formulations is a promising treatment strategy for periodontitis.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Escherichia coli/efeitos dos fármacos
Hidrogel de Polietilenoglicol-Dimetacrilato/química
Fármacos Fotossensibilizantes/farmacologia
Staphylococcus aureus/efeitos dos fármacos
Cloreto de Tolônio/farmacologia
[Mh] Termos MeSH secundário: Resinas Acrílicas/química
Antibacterianos/química
Antibacterianos/uso terapêutico
Liberação Controlada de Fármacos/efeitos da radiação
Estabilidade de Medicamentos
Infecções por Escherichia coli/tratamento farmacológico
Seres Humanos
Luz
Fotoquimioterapia
Fármacos Fotossensibilizantes/química
Fármacos Fotossensibilizantes/uso terapêutico
Infecções Estafilocócicas/tratamento farmacológico
Propriedades de Superfície
Fatores de Tempo
Cloreto de Tolônio/química
Cloreto de Tolônio/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acrylic Resins); 0 (Anti-Bacterial Agents); 0 (Photosensitizing Agents); 0 (carbomer); 15XUH0X66N (Tolonium Chloride); 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE


  2 / 1562 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28549584
[Au] Autor:Lan T; Zhao H; Xiang B; Wang J; Liu Y
[Ad] Endereço:Department of Orthodontics, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
[Ti] Título:Suture compression induced midpalatal suture chondrocyte apoptosis with increased caspase-3, caspase-9, Bad, Bak, Bax and Bid expression.
[So] Source:Biochem Biophys Res Commun;489(2):179-186, 2017 Jul 22.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Previous studies found bone resorption and chondrocytes loss in mouse models of mid-palatal suture when given continuous compressive force, although chondrocytes response remained unknown. Herein, we design this study to determine how continuous compression force induces chondrocytes apoptosis. METHODS: Thirty C57BL/6 male mice (aged 6 weeks) were randomly assigned into controls (not ligated to a spring), blank controls (ligated with no compression) and the compression group (ligated with 20-g compression). After 4 d, palatal tissues were sampled and stained by TB and safranin-O. Tunel staining measured the percentage of apoptotic chondrocytes, and immunohistochemistry was performed to label apoptosis-associated proteins (e.g., Bcl-2, Bcl-xl, Bax, Bak, Bid, Bad, caspase-3, caspase-8 and caspase-9). Intergroup comparison was made by the rank sum test, and P < 0.05 was defined as statistical significance. RESULTS: After 7d of induction, TB and safranin-O staining revealed that the cartilage area in the compression group was significantly decreased, while the control group remained largely unaltered. Tunel staining showed that apoptotic cell numbers in the mid-palatal suture were significantly higher than the control group. Immunohistochemistry showed that mice in the compression group had significantly increased expression of caspase-3, caspase-9, Bad, Bak, Bax and Bid; However, caspase-8 remained unaltered. No expression of Bcl-2 and Bcl-xl was detected. CONCLUSIONS: Continuous compression force induces chondrocytes apoptosis in the mid-palatal suture. This process might be associated with the mitochondrial pathway.
[Mh] Termos MeSH primário: Apoptose
Condrócitos/metabolismo
Condrócitos/patologia
Pressão/efeitos adversos
Suturas/efeitos adversos
Regulação para Cima
[Mh] Termos MeSH secundário: Animais
Proteína Agonista de Morte Celular de Domínio Interatuante com BH3/biossíntese
Caspase 3/biossíntese
Caspase 9/biossíntese
Imuno-Histoquímica
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Fenazinas
Cloreto de Tolônio
Proteína Killer-Antagonista Homóloga a bcl-2/biossíntese
Proteína X Associada a bcl-2/biossíntese
Proteína de Morte Celular Associada a bcl/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BH3 Interacting Domain Death Agonist Protein); 0 (Bad protein, mouse); 0 (Bak1 protein, mouse); 0 (Bax protein, mouse); 0 (Bid protein, mouse); 0 (Phenazines); 0 (bcl-2 Homologous Antagonist-Killer Protein); 0 (bcl-2-Associated X Protein); 0 (bcl-Associated Death Protein); 15XUH0X66N (Tolonium Chloride); EC 3.4.22.- (Casp3 protein, mouse); EC 3.4.22.- (Casp9 protein, mouse); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 9); XTX0YXU2HV (safranine T)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170528
[St] Status:MEDLINE


  3 / 1562 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Burger, Eva
Texto completo
[PMID]:28349345
[Au] Autor:Dos Santos LFM; Melo NB; de Carli ML; Mendes ACSC; Bani GMAC; Verinaud LM; Burger E; de Oliveira I Moraes G; Pereira AAC; Brigagão MRL; Hanemann JAC; Sperandio FF
[Ad] Endereço:School of Dentistry, Federal University of Alfenas (UNIFAL-MG), Alfenas, MG, 37130-000, Brazil.
[Ti] Título:Photodynamic inactivation of Paracoccidioides brasiliensis helps the outcome of oral paracoccidiodomycosis.
[So] Source:Lasers Med Sci;32(4):921-930, 2017 May.
[Is] ISSN:1435-604X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The antifungal drug therapy often employed to treat paracoccidiodomycosis (PCM), an important neglected fungal systemic infection, leads to offensive adverse effects, besides being very long-lasting. In addition, PCM compromises the oral health of patients by leading to oral lesions that are very painful and disabling. In that way, photodynamic therapy (PDT) arises as a new promising adjuvant treatment for inactivating Paracoccidioides brasiliensis (Pb), the responsible fungus for PCM, and also for helping the patients to deal with such debilitating oral lesions. PDT has been linked to an improved microbial killing, also presenting the advantage of not inducing immediate microbial resistance such as drugs. For the present study, we investigated the generation of reactive oxygen species (ROS) by using the fluorescent probes hydroxyphenyl fluorescein (HPF) and aminophenyl fluorescein (APF) after toluidine blue (TBO-37.5 mg/L)-mediated PDT (660 nm, 40 mW, and 0.04 cm spot area) and the action of TBO-PDT upon Pb cultures grown for 7 or 15 days in semisolid Fava Netto's culture medium; we also targeted oral PCM manifestations by reporting the first clinical cases (three patients) to receive topic PDT for such purpose. We were able to show a significant generation of hydroxyl radicals and hypochlorite after TBO-PDT with doses around 90 J/cm ; such ROS generation was particularly useful to attack and inactivate Pb colonies at 7 and 15 days. All three patients reported herein related an immediate relief when it came to pain, mouth opening, and also the ability to chew and swallow. As extracted from our clinical results, which are in fact based on in vitro outcomes, TBO-PDT is a very safe, inexpensive, and promising therapy for the oral manifestations of PCM.
[Mh] Termos MeSH primário: Viabilidade Microbiana/efeitos dos fármacos
Doenças da Boca/tratamento farmacológico
Doenças da Boca/microbiologia
Paracoccidioides/efeitos da radiação
Paracoccidioidomicose/tratamento farmacológico
Paracoccidioidomicose/microbiologia
Fotoquimioterapia
Cloreto de Tolônio/uso terapêutico
[Mh] Termos MeSH secundário: Adulto
Antifúngicos/farmacologia
Corantes Fluorescentes/química
Seres Humanos
Cinética
Masculino
Meia-Idade
Doenças da Boca/patologia
Paracoccidioides/crescimento & desenvolvimento
Espécies Reativas de Oxigênio/metabolismo
Cloreto de Tolônio/farmacologia
[Pt] Tipo de publicação:CASE REPORTS; CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Fluorescent Dyes); 0 (Reactive Oxygen Species); 15XUH0X66N (Tolonium Chloride)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170329
[St] Status:MEDLINE
[do] DOI:10.1007/s10103-017-2193-y


  4 / 1562 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28272084
[Au] Autor:Donaldson MR; Weber LA
[Ad] Endereço:Mohs Micrographic Surgery, Mountain West Dermatology, Grand Junction, Colorado.
[Ti] Título:Toluidine Blue Supports Differentiation of Folliculocentric Basaloid Proliferation From Basal Cell Carcinoma on Frozen Sections in a Small Single-Practice Cohort.
[So] Source:Dermatol Surg;43(10):1303-1306, 2017 10.
[Is] ISSN:1524-4725
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Carcinoma Basocelular/patologia
Corantes
Secções Congeladas
Neoplasias Cutâneas/patologia
Cloreto de Tolônio
[Mh] Termos MeSH secundário: Carcinoma Basocelular/cirurgia
Estudos de Coortes
Seres Humanos
Cirurgia de Mohs
Neoplasias Cutâneas/cirurgia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coloring Agents); 15XUH0X66N (Tolonium Chloride)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE
[do] DOI:10.1097/DSS.0000000000001107


  5 / 1562 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28259957
[Au] Autor:Li X; Liu Z; Liu H; Chen X; Liu Y; Tan H
[Ad] Endereço:Department of Dermatology, The Fourth Hospital of Zhenjiang, The Fourth Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China.
[Ti] Título:Photodynamic inactivation of fibroblasts and inhibition of Staphylococcus epidermidis adhesion and biofilm formation by toluidine blue O.
[So] Source:Mol Med Rep;15(4):1816-1822, 2017 Apr.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Treating skin and soft tissue infections of severe limb traumas can be challenging. Crucial concerns focus on inhibiting biofilm formation by antibiotic­resistant bacteria, and preventing scar formation by fibroblastic hyperproliferation. The local use of toluidine blue O (TBO)­mediated photodynamic therapy (PDT) may be a promising strategy for treating such lesions. The present study used Staphylococcus epidermidis (strain ATCC 35984) to assess the effects of TBO­PDT on bacterial adherence and biofilm formation, using confocal laser scanning microscopy (CLSM), tissue culture plating (TCP) and scanning electron microscopy (SEM). Primary human fibroblast cells were used to evaluate the cytotoxicity of TBO­PDT using the 3­(4,5­dimethylthiazol­2­yl)­2,5­diphenyltetrazolium bromide (MTT) assay and CLSM. Six different treatment groups were investigated: Medium only [tryptone soy broth (TSB) or Dulbecco's modified Eagle's medium (DMEM)]; red light control (light dose, 30 J/cm2); TBO group (50 mM TBO); TBO­PDT1 (TBO irradiated with 10 J/cm2); TBO­PDT2 (TBO irradiated with 20 J/cm2); and TBO­PDT3 (TBO irradiated with 30 J/cm2). The results of the S. epidermidis adhesion assay indicated that the TSB, light and TBO groups exhibited significant bacterial adherence, compared with the TBO­PDT groups. Analysis of biofilm formation revealed significant light dose­dependent differences between the TBO­PDT groups and the TSB, light, and TBO groups. Furthermore, SEM indicated fewer colony masses in the TBO­PDT groups compared with the control groups. The MTT assay for fibroblastic cell toxicity demonstrated ~1.1, 4.6, 14.5, 29.7 and 43.4% reduction in optical density for the light, TBO, TBO­PDT1, TBO­PDT2 and TBO­PDT3 groups, respectively, compared with the DMEM control group. There was no difference in toxicity between the light and control groups, however, there were significant differences among the TBO­PDT groups. Finally, alterations in fibroblast morphology and cell spreading were revealed by CLSM, following TBO­PDT treatment. TBO­PDT inhibited bacterial adhesion and biofilm formation, and exhibited significant cytotoxic effects on human fibroblasts. These results indicate that the local use of TBO­PDT in limb lesions may be a useful treatment method for inhibiting bacterial biofilm formation and fibroblastic hyperproliferation, which may prevent infectious hypertrophic scar formation.
[Mh] Termos MeSH primário: Fibroblastos/efeitos dos fármacos
Fotoquimioterapia
Fármacos Fotossensibilizantes/farmacologia
Infecções Cutâneas Estafilocócicas/tratamento farmacológico
Staphylococcus epidermidis/efeitos dos fármacos
Cloreto de Tolônio/farmacologia
[Mh] Termos MeSH secundário: Animais
Aderência Bacteriana/efeitos dos fármacos
Biofilmes/efeitos dos fármacos
Linhagem Celular
Células Cultivadas
Seres Humanos
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosensitizing Agents); 15XUH0X66N (Tolonium Chloride)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170512
[Lr] Data última revisão:
170512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170306
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2017.6184


  6 / 1562 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28040502
[Au] Autor:Asnaashari M; Ashraf H; Rahmati A; Amini N
[Ad] Endereço:Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
[Ti] Título:A comparison between effect of photodynamic therapy by LED and calcium hydroxide therapy for root canal disinfection against Enterococcus faecalis: A randomized controlled trial.
[So] Source:Photodiagnosis Photodyn Ther;17:226-232, 2017 Mar.
[Is] ISSN:1873-1597
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Insufficient root canal disinfection is one of the main reasons for persistent periapical pathology. Photodynamic therapy (PDT) has been proven effective in disinfecting infected root canals. The aim of this study was to evaluate the antimicrobial effect of photo activated disinfection (PAD) when using toluidine blue as photosensitizer and a LED lamp after the conventional treatment, and comparing it with calcium hydroxide therapy in vivo. METHODS: This clinical trial includes 20 patients with molars requiring endodontic retreatment. After the conventional treatment, first microbiological samples were obtained using sterile rotary ProTaper F2 file and 3 paper points and transferred to a microbiology laboratory. Group 1 (n=10) specimens underwent PAD with photosensitizer (PS) solution (0.1mg/mL TB) and irradiation with Fotosan light emitting diode (LED) lamp (635nm, 200mW/cm2) for 60s. Creamy Ca(OH)2 paste was used in group 2 (n=10) for two weeks. A second sample was then obtained. The samples were cultured and then bacterial colonies were counted. Data included number of colony forming units (CFUs) before and after treatments, analyzed by t-test and analysis of covariance (ANCOVA) using SPSS vs.18. RESULTS: A significant difference between results of before and after treatment of both groups (calcium hydroxide therapy p=0.02<0.05, PAD p<0.0001) indicated the efficacy of both treatments. The mean numbers for log 10CFUs/mL before calcium hydroxide therapy and PAD with LED irradiation was 10.1968 and 11.3773. After treatment, the mean numbers were 9.4202 and 8.3772, respectively. The difference in results after treatment between groups was significant (p=0.01<0.05) and indicate that PAD was more effective. CONCLUSION: PAD and calcium hydroxide therapy, as auxiliary methods adjunct to conventional root canal therapy, are both effective in root canal disinfection. In comparison with calcium hydroxide therapy, PAD leads to a greater reduction in enterococcus faecalis number in the infected root canals.
[Mh] Termos MeSH primário: Hidróxido de Cálcio/uso terapêutico
Cavidade Pulpar/microbiologia
Enterococcus faecalis/efeitos dos fármacos
Fotoquimioterapia/métodos
Fármacos Fotossensibilizantes/uso terapêutico
Cloreto de Tolônio/uso terapêutico
[Mh] Termos MeSH secundário: Sobrevivência Celular
Desinfecção/métodos
Seres Humanos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Photosensitizing Agents); 15XUH0X66N (Tolonium Chloride); PF5DZW74VN (Calcium Hydroxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170102
[St] Status:MEDLINE


  7 / 1562 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28012807
[Au] Autor:Ribeiro CM; Caixeta CA; de Carli ML; Sperandio FF; de Sá Magalhães EM; Costa Pereira AA; Costa Hanemann JA
[Ad] Endereço:Department of Clinic and Surgery, School of Dentistry, Federal University of Alfenas, Rua Gabriel Monteiro da Silva, 700-Alfenas, MG, 37130-000, Brazil. Electronic address: cinthiamribeiro@hotmail.com.
[Ti] Título:Photodynamic inactivation of oral paracoccidioidomycosis affecting woman with systemic lupus erythematosus: An unusual case report.
[So] Source:Photodiagnosis Photodyn Ther;17:160-163, 2017 Mar.
[Is] ISSN:1873-1597
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Paracoccidioidomycosis (PCM) is a systemic disease caused by the dimorphic fungus Paracocdioides brasiliensis. The infection primarily reaches the lungs by the inhalation of fungi spores and later can disseminate to other organs causing secondary oral lesions. Systemic lupus erythematosus (SLE), on the other hand, is an autoimmune chronic inflammatory disease that affects various organ systems, including skin and oral cavity. Here we report a 39-year-old female patient bearing SLE and presenting an ulcerated lesion on the hard palate extending to the superior alveolar ridge, diagnosed as PCM. Itraconazole 200mg was prescribed and photodynamic therapy (PDT) was also instituted in a way to help dealing with the PCM infection while assisting such an immunocompromised patient to heal. PDT consisted of topically placing toluidine blue dye at 37.5mg/L for 5min, followed by low-level laser irradiation (660nm; 100J/cm ; 40mW of power; 100s per point). Forty days after beginning the treatment, the patient showed total regression of the oral lesion and absence of painful symptoms. The serologic test was performed again after six months of therapy and was negative; the patient continues to be followed periodically.
[Mh] Termos MeSH primário: Paracoccidioidomicose/tratamento farmacológico
Fotoquimioterapia/métodos
Fármacos Fotossensibilizantes/uso terapêutico
Cloreto de Tolônio/uso terapêutico
[Mh] Termos MeSH secundário: Adulto
Antifúngicos/uso terapêutico
Terapia Combinada
Feminino
Seres Humanos
Hospedeiro Imunocomprometido
Itraconazol/uso terapêutico
Lúpus Eritematoso Sistêmico/epidemiologia
Paracoccidioidomicose/epidemiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Photosensitizing Agents); 15XUH0X66N (Tolonium Chloride); 304NUG5GF4 (Itraconazole)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161226
[St] Status:MEDLINE


  8 / 1562 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28006679
[Au] Autor:Moon JM; Kim DM; Kim MH; Han JY; Jung DK; Shim YB
[Ad] Endereço:Department of Chemistry, Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan 46241, South Korea.
[Ti] Título:A disposable amperometric dual-sensor for the detection of hemoglobin and glycated hemoglobin in a finger prick blood sample.
[So] Source:Biosens Bioelectron;91:128-135, 2017 May 15.
[Is] ISSN:1873-4235
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A disposable microfluidic amperometric dual-sensor was developed for the detection of glycated hemoglobin (HbA ) and total hemoglobin (Hb), separately, in a finger prick blood sample. The accurate level of total Hb was determined through the measurements of the cathodic currents of total Hb catalyzed by a toluidine blue O (TBO)-modified working electrode. Subsequently, after washing unbound Hb in the fluidic channel of dual sensor with PBS, the cathodic current by only HbA captured on aptamer was monitored using another aptamer/TBO-modified working electrode in the channel. To modify the sensor probe, poly(2,2´:5´,5″-terthiophene-3´-p-benzoic acid) and a multi-wall carbon nanotube (MWCNT) composite layer (pTBA@MWCNT) was electropolymerized on a screen printed carbon electrode (SPCE), followed by immobilization of TBO for the total Hb probe and aptamer/TBO for the HbA probe, respectively. The characterization of each sensor surface was performed using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), X-ray photoelectron spectroscopy (XPS), quartz crystal microbalance (QCM), field-emission scanning electron microscopy (FE-SEM), and transmission electron microscopy (TEM). The experimental conditions affecting the analytical signal were optimized in terms of the amount of TBO, pH, temperature, binding time, applied potential, and the content ratio of monomer and MWCNT. The dynamic ranges of Hb and HbA were from 0.1 to 10µM and from 0.006 to 0.74µM, with detection limits of 82(±4.2)nM and 3.7(±0.8)nM, respectively. The reliability of the proposed microfluidic dual-sensor for a finger prick blood sample (1µL) was evaluated in parallel with a conventional method (HPLC) for point-of-care analysis.
[Mh] Termos MeSH primário: Aptâmeros de Nucleotídeos/química
Técnicas Biossensoriais/instrumentação
Técnicas Eletroquímicas/instrumentação
Hemoglobina A Glicada/análise
Hemoglobinas/análise
Técnicas Analíticas Microfluídicas/instrumentação
[Mh] Termos MeSH secundário: Técnicas Biossensoriais/economia
Corantes/química
Técnicas Eletroquímicas/economia
Eletrodos
Seres Humanos
Técnicas Analíticas Microfluídicas/economia
Modelos Moleculares
Sistemas Automatizados de Assistência Junto ao Leito/economia
Reprodutibilidade dos Testes
Cloreto de Tolônio/química
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aptamers, Nucleotide); 0 (Coloring Agents); 0 (Glycated Hemoglobin A); 0 (Hemoglobins); 15XUH0X66N (Tolonium Chloride)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161223
[St] Status:MEDLINE


  9 / 1562 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27890593
[Au] Autor:Pourhajibagher M; Bahador A
[Ad] Endereço:Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran; Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
[Ti] Título:Evaluation of the crystal structure of a fimbrillin (FimA) from Porphyromonas gingivalis as a therapeutic target for photo-activated disinfection with toluidine blue O.
[So] Source:Photodiagnosis Photodyn Ther;17:98-102, 2017 Mar.
[Is] ISSN:1873-1597
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Porphyromonas gingivalis is one of the etiological agents in the initiation of combined periodontal-endodontic (perio-endo) lesions. Successful treatment of perio-endo lesions with photo-activated disinfection (PAD) as a novel therapeutic approach depends on the selection of an appropriate target site, specific photosensitizer (PS), and wavelength of light. Since the fimbrillin (FimA) is the most important pathogenic agent in P. gingivalis it was evaluated as a target site against PAD with toluidine blue O (TBO) as a PS. MATERIALS AND METHODS: We evaluated the potential of FimA using a number of bioinformatic tools and computer simulation molecular modeling. In silico analysis was done to predict the hierarchical nature of structure, topology, backbone, physicochemical properties, and functional characterization of FimA. RESULTS: The predicted structure of FimA exhibited that it is an anionic protein with negative charge (-7.00) in pH7 and random coil which dominates other secondary structures located outside the cell. It has three motifs with seven ligand binding site residues and one active site residue which can be a site of interaction for cationic TBO. CONCLUSION: Based on the results of this study cationic TBO tends to interact with FimA during PAD as a major target to increase the efficiency of treatment of perio-endo lesions.
[Mh] Termos MeSH primário: Proteínas de Fímbrias/efeitos dos fármacos
Fotoquimioterapia/métodos
Fármacos Fotossensibilizantes/farmacologia
Porphyromonas gingivalis/efeitos dos fármacos
Cloreto de Tolônio/farmacologia
[Mh] Termos MeSH secundário: Simulação por Computador
Desinfecção/métodos
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosensitizing Agents); 0 (fimbrillin); 147680-16-8 (Fimbriae Proteins); 15XUH0X66N (Tolonium Chloride)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161129
[St] Status:MEDLINE


  10 / 1562 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27769914
[Au] Autor:Beytollahi L; Pourhajibagher M; Chiniforush N; Ghorbanzadeh R; Raoofian R; Pourakbari B; Bahador A
[Ad] Endereço:Department of Microbiology, School of Medicine, International Campus, Tehran University of Medical Sciences (IC-TUMS), Tehran, Iran.
[Ti] Título:The efficacy of photodynamic and photothermal therapy on biofilm formation of Streptococcus mutans: An in vitro study.
[So] Source:Photodiagnosis Photodyn Ther;17:56-60, 2017 Mar.
[Is] ISSN:1873-1597
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The alternative antibacterial treatments of photodynamic therapy (PDT) and photothermal therapy (PTT) significantly affect microbiota inactivation. The aim of the present research was the assessment of the antimicrobial and anti-biofilm effects of PDT with toluidine blue O (TBO) and PTT with indocyanine green (ICG) on Streptococcus mutans as a cariogenic bacterium. MATERIALS AND METHODS: The S. mutans ATCC 35668 strain was treated with final concentrations of 0.1mg/mL TBO and 1mg/mL ICG with energy densities of 17.18 and 15.62J/cm , respectively. Cell viability was evaluated after culturing and anti-biofilm potential was analyzed using crystal violet assay and scanning electron microscopy. RESULTS: The number of S. mutans colony forming unit (CFU)/mL was significantly lower in the groups submitted to PDT (12.5-100µg/mL TBO) and PTT (62.5-1000µg/mL) compared to the control (untreated group). 0.1mg/mL TBO-PDT and 1mg/mL ICG-PTT showed stronger inhibitory effects on biofilm formation in S. mutans than other concentration levels, with a reduction of 63.87% and 67.3%, respectively. CONCLUSION: Photo-elimination by high concentrations of TBO-PDT and ICG-PTT exhibited significantly stronger inhibitory effects on biofilm formation and cell viability in S. mutans.
[Mh] Termos MeSH primário: Biofilmes/efeitos dos fármacos
Fármacos Fotossensibilizantes/farmacologia
Fototerapia/métodos
Streptococcus mutans/efeitos dos fármacos
[Mh] Termos MeSH secundário: Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Seres Humanos
Verde de Indocianina/administração & dosagem
Verde de Indocianina/farmacologia
Lasers Semicondutores
Fotoquimioterapia/métodos
Fármacos Fotossensibilizantes/administração & dosagem
Cloreto de Tolônio/administração & dosagem
Cloreto de Tolônio/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Photosensitizing Agents); 15XUH0X66N (Tolonium Chloride); IX6J1063HV (Indocyanine Green)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE



página 1 de 157 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde