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Referências encontradas : 277 [refinar]
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[PMID]:29276826
[Au] Autor:Osawa R; Kamide T; Satoh Y; Kawano Y; Ohtsu I; Dairi T
[Ti] Título:Heterologous and High Production of Ergothioneine in Escherichia coli.
[So] Source:J Agric Food Chem;66(5):1191-1196, 2018 Feb 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ergothioneine (ERG) is a histidine-derived thiol compound suggested to function as an antioxidant and cytoprotectant in humans. Therefore, experimental trials have been conducted applying ERG from mushrooms in dietary supplements and as a cosmetic additive. However, this method of producing ERG is expensive; therefore, alternative methods for ERG supply are required. Five Mycobacterium smegmatis genes, egtABCDE, have been confirmed to be responsible for ERG biosynthesis. This enabled us to develop practical fermentative ERG production by microorganisms. In this study, we carried out heterologous and high-level production of ERG in Escherichia coli using the egt genes from M. smegmatis. By high production of each of the Egt enzymes and elimination of bottlenecks in the substrate supply, we succeeded in constructing a production system that yielded 24 mg/L (104 µM) secreted ERG.
[Mh] Termos MeSH primário: Ergotioneína/biossíntese
Escherichia coli/metabolismo
[Mh] Termos MeSH secundário: Antioxidantes
Citoproteção
Escherichia coli/genética
Fermentação
Técnicas de Transferência de Genes
Mycobacterium smegmatis/crescimento & desenvolvimento
Proteínas Recombinantes/biossíntese
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Recombinant Proteins); BDZ3DQM98W (Ergothioneine)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171226
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04924


  2 / 277 MEDLINE  
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[PMID]:28530594
[Au] Autor:Kalaras MD; Richie JP; Calcagnotto A; Beelman RB
[Ad] Endereço:Center for Plant and Mushroom Foods for Health, Department of Food Science, The Pennsylvania State University, 202 Rodney A. Erickson Food Science Building, University Park, PA 16802, United States. Electronic address: mdk219@psu.edu.
[Ti] Título:Mushrooms: A rich source of the antioxidants ergothioneine and glutathione.
[So] Source:Food Chem;233:429-433, 2017 Oct 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:While mushrooms are the highest dietary source for the unique sulfur-containing antioxidant ergothioneine, little is known regarding levels of the major biological antioxidant glutathione. Thus, our objectives were to determine and compare levels of glutathione, as well as ergothioneine, in different species of mushrooms. Glutathione levels varied >20-fold (0.11-2.41mg/gdw) with some varieties having higher levels than reported for other foods. Ergothioneine levels also varied widely (0.15-7.27mg/gdw) and were highly correlated with those of glutathione (r=0.62, P<0.001). Both antioxidants were more concentrated in pileus than stipe tissues in selected mushrooms species. Agaricus bisporus harvested during the third cropping flush contained higher levels of ergothioneine and glutathione compared to the first flush, possibly as a response to increased oxidative stress. This study demonstrated that certain mushroom species are high in glutathione and ergothioneine and should be considered an excellent dietary source of these important antioxidants.
[Mh] Termos MeSH primário: Agaricales/química
[Mh] Termos MeSH secundário: Antioxidantes
Dieta
Ergotioneína
Glutationa
Estresse Oxidativo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); BDZ3DQM98W (Ergothioneine); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE


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[PMID]:28530575
[Au] Autor:Pahila J; Kaneda H; Nagasaka R; Koyama T; Ohshima T
[Ad] Endereço:Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato-ku, Tokyo 108-8477, Japan.
[Ti] Título:Effects of ergothioneine-rich mushroom extracts on lipid oxidation and discoloration in salmon muscle stored at low temperatures.
[So] Source:Food Chem;233:273-281, 2017 Oct 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The effects of supplementing ergothioneine-rich mushroom extracts (ME) on discoloration and lipid oxidation in astaxanthin-pigmented salmon muscles were evaluated. ME (Flammulina velutipes) were added (in vitro) to minced Oncorhynchus mykiss muscles and stored at -10°C. During several weeks of storage, the ME-treated group showed lower lipid hydroperoxide (HPO) accumulation and higher retained astaxanthin levels than in the control group. The effects of adding concentrated ME (Pleurotus cornucopiae) to Oncorhynchus kisutch diets were also tested over a 2-month feeding trial. No adverse effects on fish growth or pigmentation were observed. Muscle samples were collected, stored (-2 or -18°C), and evaluated. At the end of the storage period, the ME-treated group had lower HPO and higher retained astaxanthin levels in the muscle samples than the levels in the control group. Thus, the addition of ergothioneine from MEs successfully controlled lipid oxidation and stabilized astaxanthin during post-harvest storage at low temperatures.
[Mh] Termos MeSH primário: Flammulina
[Mh] Termos MeSH secundário: Animais
Ergotioneína
Oxirredução
Salmão
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
BDZ3DQM98W (Ergothioneine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE


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[PMID]:28114402
[Au] Autor:Yoshida S; Shime H; Funami K; Takaki H; Matsumoto M; Kasahara M; Seya T
[Ad] Endereço:Department of Microbiology and Immunology, Graduate School of Medicine, Hokkaido University, Kita-ku, Sapporo City, Japan.
[Ti] Título:The Anti-Oxidant Ergothioneine Augments the Immunomodulatory Function of TLR Agonists by Direct Action on Macrophages.
[So] Source:PLoS One;12(1):e0169360, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:L-Ergothioneine (EGT) is a naturally-occurring amino acid which is characterized by its antioxidant property; yet, the physiological role of EGT has yet to be established. We investigated the immune-enhancing properties of EGT, and found that it acts as a potentiator of toll-like receptor (TLR) signaling. When mouse bone marrow-derived macrophages (BMDMs) were pretreated with EGT, TLR signal-mediated cytokine production was augmented in BMDMs. The results were reproducible with TLR2, 3, 4 and 7 agonists. In particular, IL-6 and IL-12p40 were elevated further by pretreatment with EGT in BMDMs, suggesting the induction of M1 polarization. In co-culture assay with OT-II CD4+ T cells and splenic F4/80+ macrophages, EGT significantly induced Th17 skewing in CD4+ T cells. Thus, EGT is an immune modifier as well as a redox controller under TLR stimulation that induces M1 macrophages and a Th17 shift in inflammation.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/farmacologia
Antioxidantes/farmacologia
Ergotioneína/farmacologia
Macrófagos/efeitos dos fármacos
Receptores Toll-Like/agonistas
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Citocinas/biossíntese
Feminino
Macrófagos/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antioxidants); 0 (Cytokines); 0 (Reactive Oxygen Species); 0 (Toll-Like Receptors); BDZ3DQM98W (Ergothioneine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0169360


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[PMID]:27488221
[Au] Autor:Cheah IK; Tang RM; Yew TS; Lim KH; Halliwell B
[Ad] Endereço:1 Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore , Singapore .
[Ti] Título:Administration of Pure Ergothioneine to Healthy Human Subjects: Uptake, Metabolism, and Effects on Biomarkers of Oxidative Damage and Inflammation.
[So] Source:Antioxid Redox Signal;26(5):193-206, 2017 Feb 10.
[Is] ISSN:1557-7716
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AIM: We investigated the uptake and pharmacokinetics of l-ergothioneine (ET), a dietary thione with free radical scavenging and cytoprotective capabilities, after oral administration to humans, and its effect on biomarkers of oxidative damage and inflammation. RESULTS: After oral administration, ET is avidly absorbed and retained by the body with significant elevations in plasma and whole blood concentrations, and relatively low urinary excretion (<4% of administered ET). ET levels in whole blood were highly correlated to levels of hercynine and S-methyl-ergothioneine, suggesting that they may be metabolites. After ET administration, some decreasing trends were seen in biomarkers of oxidative damage and inflammation, including allantoin (urate oxidation), 8-hydroxy-2'-deoxyguanosine (DNA damage), 8-iso-PGF2α (lipid peroxidation), protein carbonylation, and C-reactive protein. However, most of the changes were non-significant. INNOVATION: This is the first study investigating the administration of pure ET to healthy human volunteers and monitoring its uptake and pharmacokinetics. This compound is rapidly gaining attention due to its unique properties, and this study lays the foundation for future human studies. CONCLUSION: The uptake and retention of ET by the body suggests an important physiological function. The decreasing trend of oxidative damage biomarkers is consistent with animal studies suggesting that ET may function as a major antioxidant but perhaps only under conditions of oxidative stress. Antioxid. Redox Signal. 26, 193-206.
[Mh] Termos MeSH primário: Antioxidantes/administração & dosagem
Biomarcadores
Ergotioneína/administração & dosagem
Estresse Oxidativo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Alantoína/metabolismo
Antioxidantes/química
Antioxidantes/farmacocinética
Betaína/análogos & derivados
Betaína/metabolismo
Proteína C-Reativa/metabolismo
Desoxiguanosina/análogos & derivados
Desoxiguanosina/metabolismo
Monitoramento de Medicamentos
Ergotioneína/química
Ergotioneína/farmacocinética
Voluntários Saudáveis
Histidina/análogos & derivados
Histidina/metabolismo
Seres Humanos
Inflamação/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biomarkers); 344S277G0Z (Allantoin); 3SCV180C9W (Betaine); 4QD397987E (Histidine); 88847-89-6 (8-oxo-7-hydrodeoxyguanosine); 9007-41-4 (C-Reactive Protein); BDZ3DQM98W (Ergothioneine); G9481N71RO (Deoxyguanosine); M8MWM6K25V (hercynine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170810
[Lr] Data última revisão:
170810
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160805
[St] Status:MEDLINE
[do] DOI:10.1089/ars.2016.6778


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[PMID]:27444382
[Au] Autor:Cheah IK; Feng L; Tang RMY; Lim KHC; Halliwell B
[Ad] Endereço:Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 28 Medical Drive, Singapore 117456, Singapore.
[Ti] Título:Ergothioneine levels in an elderly population decrease with age and incidence of cognitive decline; a risk factor for neurodegeneration?
[So] Source:Biochem Biophys Res Commun;478(1):162-167, 2016 09 09.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ergothioneine (ET), a naturally occurring thione, can accumulate in the human body at high concentrations from diet. Following absorption via a specific transporter, OCTN1, ET may accumulate preferentially in tissues predisposed to higher levels of oxidative stress and inflammation. Given its potential cytoprotective effects, we examined how ET levels change with age. We found that whole blood ET levels in elderly individuals decline significantly beyond 60 years of age. Additionally, a subset of these subjects with mild cognitive impairment had significantly lower plasma ET levels compared with age-matched subjects. This decline suggests that deficiency in ET may be a risk factor, predisposing individuals to neurodegenerative diseases.
[Mh] Termos MeSH primário: Envelhecimento/metabolismo
Disfunção Cognitiva/sangue
Disfunção Cognitiva/epidemiologia
Ergotioneína/sangue
Doenças Neurodegenerativas/sangue
Doenças Neurodegenerativas/epidemiologia
[Mh] Termos MeSH secundário: Distribuição por Idade
Idoso
Idoso de 80 Anos ou mais
Biomarcadores/sangue
Disfunção Cognitiva/diagnóstico
Feminino
Seres Humanos
Incidência
Meia-Idade
Doenças Neurodegenerativas/diagnóstico
Reprodutibilidade dos Testes
Medição de Risco/métodos
Fatores de Risco
Sensibilidade e Especificidade
Singapura/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); BDZ3DQM98W (Ergothioneine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160723
[St] Status:MEDLINE


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[PMID]:27350110
[Au] Autor:Futatsugi A; Masuo Y; Kawabata S; Nakamichi N; Kato Y
[Ad] Endereço:Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Japan.
[Ti] Título:L503F variant of carnitine/organic cation transporter 1 efficiently transports metformin and other biguanides.
[So] Source:J Pharm Pharmacol;68(9):1160-9, 2016 Sep.
[Is] ISSN:2042-7158
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Carnitine/organic cation transporter 1 (OCTN1) is involved in gastrointestinal absorption and mitochondrial toxicity of biguanides in rodents, but its pharmacokinetic roles in humans are largely unknown. The purpose of this study was to clarify the transport activities of two major OCTN1 variants, L503F and I306T, for gabapentin and three biguanide drugs, metformin, buformin and phenformin. METHODS: HEK293 cells were transfected with OCTN1 gene, its variants, or vector alone, and the uptake and cytotoxicity of each drug were examined. KEY FINDINGS: Buformin was identified to be an OCTN1 substrate. Uptake of biguanides, especially metformin, mediated by OCTN1 variant L503F, which is commonly found in Caucasians, was much higher than that by the wild-type transporter (WT-OCTN1). Cytotoxicity of metformin was also greater in HEK293 cells expressing the L503F variant, compared with WT-OCTN1. Uptake of gabapentin mediated by OCTN1 variant I306T, which is commonly found in both Asians and Caucasians, was lower than that by WT-OCTN1, although uptake of the typical OCTN1 substrate ergothioneine was similar. CONCLUSION: Organic cation transporter 1 variant L503F transports biguanides, especially metformin, more efficiently than WT-OCTN1, whereas the I306T variant transports gabapentin less efficiently than WT-OCTN1, suggesting that the common OCTN1 variants may alter pharmacokinetics of these drugs.
[Mh] Termos MeSH primário: Absorção Intestinal
Metformina/farmacocinética
Transportador 1 de Cátions Orgânicos/genética
Polimorfismo de Nucleotídeo Único
[Mh] Termos MeSH secundário: Aminas/metabolismo
Aminas/farmacocinética
Grupo com Ancestrais do Continente Asiático
Biguanidas/metabolismo
Biguanidas/farmacocinética
Transporte Biológico Ativo
Buformina/metabolismo
Buformina/farmacocinética
Carnitina/metabolismo
Ácidos Cicloexanocarboxílicos/metabolismo
Ácidos Cicloexanocarboxílicos/farmacocinética
Ergotioneína/metabolismo
Ergotioneína/farmacocinética
Grupo com Ancestrais do Continente Europeu
Células HEK293
Seres Humanos
Metformina/metabolismo
Transportador 1 de Cátions Orgânicos/metabolismo
Ácido gama-Aminobutírico/metabolismo
Ácido gama-Aminobutírico/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amines); 0 (Biguanides); 0 (Cyclohexanecarboxylic Acids); 0 (Organic Cation Transporter 1); 56-12-2 (gamma-Aminobutyric Acid); 6CW7F3G59X (gabapentin); 9100L32L2N (Metformin); BDZ3DQM98W (Ergothioneine); S7UI8SM58A (Carnitine); W2115E9C7B (Buformin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160629
[St] Status:MEDLINE
[do] DOI:10.1111/jphp.12574


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[PMID]:27193019
[Au] Autor:Calvo MS; Mehrotra A; Beelman RB; Nadkarni G; Wang L; Cai W; Goh BC; Kalaras MD; Uribarri J
[Ad] Endereço:Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, MOD-1, HFS-025, 8301 Muirkirk Road, Laurel, MD, 20708, USA.
[Ti] Título:A Retrospective Study in Adults with Metabolic Syndrome: Diabetic Risk Factor Response to Daily Consumption of Agaricus bisporus (White Button Mushrooms).
[So] Source:Plant Foods Hum Nutr;71(3):245-51, 2016 Sep.
[Is] ISSN:1573-9104
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Adults with metabolic syndrome from different race/ethnicities are often predisposed to developing type 2 diabetes (T2D); however, growing evidence suggests that healthy diets and lifestyle choices can significantly slow or prevent progression to T2D. This poorly understood relationship to healthy dietary patterns and prevention of T2D motivated us to conduct a retrospective analysis to determine the potential impact of a minor dietary lifestyle change (daily mushroom consumption) on known T2D risk factors in racially diverse adults with confirmed features of the metabolic syndrome. Retrospectively, we studied 37 subjects who had participated in a dietary intervention focused on vitamin D bioavailability from white button mushrooms (WBM). All 37 had previously completed a 16-week study where they consumed 100 g of WBM daily and were then followed-up for one month during which no mushrooms were consumed. We analyzed differences in serum risk factors from baseline to 16-week, and from baseline to one-month follow-up. Measurement of serum diabetic risk factors included inflammatory and oxidative stress markers and the antioxidant component naturally rich in mushrooms, ergothioneine. Significant beneficial health effects were observed at 16-week with the doubling of ergothioneine from baseline, increases in the antioxidant marker ORAC (oxygen radical absorption capacity) and anti-inflammatory hormone, adiponectin and significant decreases in serum oxidative stress inducing factors, carboxymethyllysine (CML) and methylglyoxal (MG), but no change in the lipid oxidative stress marker 8-isoprostane, leptin or measures of insulin resistance or glucose metabolism. We conclude that WBM contain a variety of compounds with potential anti-inflammatory and antioxidant health benefits that can occur with frequent consumption over time in adults predisposed to T2D. Well-controlled studies are needed to confirm these findings and identify the specific mushroom components beneficial to health.
[Mh] Termos MeSH primário: Agaricus
Dieta
Síndrome Metabólica/dietoterapia
[Mh] Termos MeSH secundário: Adiponectina/sangue
Adulto
Antioxidantes/análise
Biomarcadores/sangue
Índice de Massa Corporal
Quitina/análise
HDL-Colesterol/sangue
LDL-Colesterol/sangue
Diabetes Mellitus Tipo 2/sangue
Diabetes Mellitus Tipo 2/dietoterapia
Dinoprosta/análogos & derivados
Dinoprosta/sangue
Ergotioneína/análise
Feminino
Seguimentos
Hemoglobina A Glicada/metabolismo
Seres Humanos
Resistência à Insulina
Leptina/sangue
Modelos Lineares
Lisina/análogos & derivados
Lisina/sangue
Masculino
Síndrome Metabólica/sangue
Meia-Idade
Análise Multivariada
Estresse Oxidativo
Polifenóis/análise
Aldeído Pirúvico/sangue
Estudos Retrospectivos
Fatores de Risco
Triglicerídeos/sangue
Vitamina D/sangue
Vitamina D/farmacocinética
beta-Glucanas/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adiponectin); 0 (Antioxidants); 0 (Biomarkers); 0 (Cholesterol, HDL); 0 (Cholesterol, LDL); 0 (Glycated Hemoglobin A); 0 (Leptin); 0 (Polyphenols); 0 (Triglycerides); 0 (beta-Glucans); 1398-61-4 (Chitin); 1406-16-2 (Vitamin D); 27415-26-5 (8-epi-prostaglandin F2alpha); 70YDX3Z2O7 (N(6)-carboxymethyllysine); 722KLD7415 (Pyruvaldehyde); B7IN85G1HY (Dinoprost); BDZ3DQM98W (Ergothioneine); K3Z4F929H6 (Lysine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160520
[St] Status:MEDLINE
[do] DOI:10.1007/s11130-016-0552-7


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[PMID]:27134772
[Au] Autor:Nakamichi N; Nakayama K; Ishimoto T; Masuo Y; Wakayama T; Sekiguchi H; Sutoh K; Usumi K; Iseki S; Kato Y
[Ad] Endereço:Faculty of Pharmacy Institute of Medical, Pharmaceutical and Health Sciences Kanazawa University Kanazawa 920-1192 Japan.
[Ti] Título:Food-derived hydrophilic antioxidant ergothioneine is distributed to the brain and exerts antidepressant effect in mice.
[So] Source:Brain Behav;6(6):e00477, 2016 Jun.
[Is] ISSN:2162-3279
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Clinically used antidepressants suffer from various side effects. Therefore, we searched for a safe antidepressant with minimal side effects among food ingredients that are distributed to the brain. Here, we focused on ERGO (ergothioneine), which is a hydrophilic antioxidant and contained at high levels in edible golden oyster mushrooms. ERGO is a typical substrate of carnitine/organic cation transporter OCTN1/SLC22A4, which is expressed in the brain and neuronal stem cells, although little is known about its permeation through the BBB (blood-brain barrier) or its neurological activity. METHODS: To clarify the exposure of ERGO to brain and the possible antidepressant-like effect after oral ingestion, ERGO or GOME (golden oyster mushroom extract) which contains 1.2% (w/w) ERGO was mixed with feed and provided to mice for 2 weeks, and then ERGO concentration and antidepressant-like effect were evaluated by LC-MS/MS and FST (forced swimming test) or TST (tail suspension test), respectively. RESULTS: Diet containing ERGO or GOME greatly increased the ERGO concentrations in plasma and brain, and significantly decreased the immobility time in both FST and TST. The required amount of GOME (~37 mg/day) to show the antidepressant-like effect corresponds to at most 8 g/day in humans. In mice receiving GOME-containing diet, doublecortin-positive cells showed a significant increase from the basal level, suggesting promotion of neuronal differentiation. CONCLUSION: Thus, orally ingested ERGO is transported across the BBB into the brain, where it may promote neuronal differentiation and alleviate symptoms of depression at plausibly achieved level of daily ingestion.
[Mh] Termos MeSH primário: Antidepressivos/farmacologia
Antioxidantes/farmacologia
Comportamento Animal/efeitos dos fármacos
Encéfalo/metabolismo
Ergotioneína/farmacologia
Extratos Vegetais/farmacologia
Pleurotus
[Mh] Termos MeSH secundário: Animais
Antidepressivos/administração & dosagem
Antidepressivos/sangue
Antioxidantes/administração & dosagem
Antioxidantes/metabolismo
Encéfalo/efeitos dos fármacos
Depressão/dietoterapia
Depressão/tratamento farmacológico
Ergotioneína/administração & dosagem
Ergotioneína/sangue
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Extratos Vegetais/administração & dosagem
Extratos Vegetais/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antidepressive Agents); 0 (Antioxidants); 0 (Plant Extracts); BDZ3DQM98W (Ergothioneine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160503
[St] Status:MEDLINE
[do] DOI:10.1002/brb3.477


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[PMID]:27067265
[Au] Autor:Sotgia S; Arru D; Sotgiu E; Mangoni AA; Forteschi M; Pintus G; Carru C; Zinellu A
[Ad] Endereço:Department of Biomedical Sciences, School of Medicine, University of Sassari, Sassari, Italy.
[Ti] Título:Simultaneous determination of the main amino thiol and thione in human whole blood by CE and LC.
[So] Source:Bioanalysis;8(9):945-51, 2016 May.
[Is] ISSN:1757-6199
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Two precolumn fluorescence derivatization procedures by two different sulfhydryl-reactive iodoacetyl reagents were established to measure simultaneously glutathione and l-ergothioneine in human whole blood by means of CE and LC. MATERIALS & METHODS: Separations were achieved in <5 min on a reverse-phase column (100 mm × 4.6 mm Zorbax Eclipse Plus C18 3.5 µm) for LC analysis, and on an uncoated fused-silica capillary (60 cm × 50 µm) for CE analysis, monitoring the fluorescence of derivatives. RESULTS: Performance of the assays was good in terms of linearity, recovery, intra- and inter-day precision and LOD and LOQ. CONCLUSION: This novel approach allows rapid assessment of circulating glutathione and l-ergothioneine concentrations for clinical and research purposes.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Eletroforese Capilar/métodos
Ergotioneína/sangue
Glutationa/sangue
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Antioxidantes/farmacocinética
Feminino
Seres Humanos
Limite de Detecção
Masculino
Meia-Idade
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); BDZ3DQM98W (Ergothioneine); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160413
[St] Status:MEDLINE
[do] DOI:10.4155/bio-2015-0002



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