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  1 / 14007 MEDLINE  
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[PMID]:29318318
[Au] Autor:Vajs J; Pevec A; Gazvoda M; Urankar D; Goreshnik E; Polanc S; Kosmrlj J
[Ti] Título:Synthesis and X-ray Structural Analysis of the Ruthenium(III) Complex Na[trans-RuCl4(DMSO) (PyrDiaz)], the Diazene Derivative of Antitumor NAMI-Pyr.
[So] Source:Acta Chim Slov;64(4):763-770, 2017 Dec.
[Is] ISSN:1318-0207
[Cp] País de publicação:Slovenia
[La] Idioma:eng
[Ab] Resumo:Novel tetrachloridoruthenium(III) complex Na[trans-RuCl4(DMSO)(PyrDiaz)] (3) with pyridine-tethered diazenedicarboxamide PyrDiaz ligand (PyrDiaz = N1-(4-isopropylphenyl)-N2-(pyridin-2-ylmethyl)diazene-1,2-dicarboxamide) was synthesized by direct coupling of PyrDiaz with sodium trans-bis(dimethyl sulfoxide)tetrachloridoruthenate(III) (Na-[trans-Ru(DMSO)2Cl4]). Compound 3 is the analogue of the antimetastatic Ru(III) complex NAMI-A and NAMI-Pyr. Single crystal X.
[Mh] Termos MeSH primário: Antineoplásicos/síntese química
Dimetil Sulfóxido/análogos & derivados
Compostos Organometálicos/química
Rutênio/química
[Mh] Termos MeSH secundário: Antineoplásicos/química
Cristalografia por Raios X
Dimetil Sulfóxido/química
Imidas/síntese química
Imidas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Imides); 0 (Organometallic Compounds); 7UI0TKC3U5 (Ruthenium); LM321PYV3Y (diazene); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE


  2 / 14007 MEDLINE  
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[PMID]:28453219
[Au] Autor:Worsham DN; Reems JA; Szczepiorkowski ZM; McKenna DH; Leemhuis T; Mathew AJ; Cancelas JA; Biomedical Excellence for Safer Transfusion (BEST) Collaborative Group
[Ad] Endereço:Hoxworth Blood Center, University of Cincinnati, Cincinnati, Ohio.
[Ti] Título:Clinical methods of cryopreservation for donor lymphocyte infusions vary in their ability to preserve functional T-cell subpopulations.
[So] Source:Transfusion;57(6):1555-1565, 2017 06.
[Is] ISSN:1537-2995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cryopreserved donor lymphocyte infusion (DLI) products are manufactured and administered to treat relapse after allogeneic hematopoietic stem cell transplantation. Reported clinical responses to DLIs vary broadly, even within the same group of patients. While there is an implicit recognition of the fact that different manufacturing protocols may have specific effects on different cell types, cryopreservation protocols are frequently derived from our experience in the cryopreservation of stem cell products and do not account for the heterogeneous functional nature of DLI T-cell populations. Here, we report the results of a prospective, multicenter trial on the effect of four different cryopreservation solutions that were used to freeze DLIs compared to control DLIs that were refrigerated overnight. STUDY DESIGN AND METHODS: Cryopreserved postthawed and refrigerated specimens were analyzed side by side for their T-cell subpopulation content and viability, as well as T-cell proliferation, cytokine secretion, and cytotoxic activities. RESULTS: This study indicates that "homemade" 10% dimethyl sulfoxide (DMSO) results in reduced viability of different CD4+ T-cell populations, including T-helper, T-cytotoxic, and T-regulatory populations, and a decrease in their proliferative and cytotoxic response to immunologically relevant stimuli, while the use of solutions containing 5% DMSO with intracellular-like cryoprotectant stabilizers maintains T-cell function at levels similar to refrigerated control samples. CONCLUSION: This study has important implications in determining the best cryoprotectant solution for specific clinical applications in allogeneic immunotherapy.
[Mh] Termos MeSH primário: Criopreservação/métodos
Transfusão de Linfócitos/métodos
[Mh] Termos MeSH secundário: Linfócitos T CD4-Positivos/citologia
Linfócitos T CD4-Positivos/imunologia
Ciclo Celular/fisiologia
Dimetil Sulfóxido/química
Transplante de Células-Tronco Hematopoéticas
Seres Humanos
Imunofenotipagem
Linfócitos T Citotóxicos/citologia
Linfócitos T Citotóxicos/imunologia
Linfócitos T Auxiliares-Indutores/citologia
Linfócitos T Auxiliares-Indutores/imunologia
Linfócitos T Reguladores/citologia
Linfócitos T Reguladores/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1111/trf.14112


  3 / 14007 MEDLINE  
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[PMID]:29442003
[Au] Autor:Li Z; Shu J; Yang B; Xu C; Zou Y; Sun W
[Ti] Título:Evaluating the relationship between cell viability and volatile organic compound production following DMSO treatment of cultured human cells.
[So] Source:Pharmazie;71(12):727-732, 2016 12 01.
[Is] ISSN:0031-7144
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Methylsulfinylmethane (dimethyl sulfoxide; DMSO) is widely used in clinical treatment and bioresearch. Moreover, there is bioconversion between methylsulfanylmethane (dimethyl sulfide; DMS), DMSO, and methylsulfonylmethane (DMSO2) in mammalian metabolism. Due to the real-time detection limits for volatile compounds, most research has focused on DMSO2 as a stable byproduct of DMSO. Therefore, details about the production of DMS as a byproduct of DMSO metabolism remain to be elucidated. Here, we report the characterization of trace-level volatile organic compounds (VOCs) produced following DMSO treatment of cultured human cells using an ultrasensitive vacuum ultraviolet photoionization mass spectrometer (VUV-PIMS). Using this approach, 24 h after DMSO treatment we detected 16.9 and 21 parts per billion by volume (ppbv) DMS in the atmosphere above the cells (headspace) within HeLa and 293T tissue culture flasks, respectively. When simultaneously exposed to 50 nM paclitaxel (PTX), 17.6 and 22.3 ppbv DMS were detected in the headspace of HeLa and 293T culture flasks, respectively. Nevertheless, at doses of PTX more or less than 50 nM, the detectable levels of DMS were reduced to as low as 8.4 ppbv. Our experimental results demonstrate that by co-administering 5 to 10 nM PTX with DMSO, it is possible to moderate the production of DMS considerably. However, at higher doses of PTX, increased apoptosis was observed that likely contributed to higher DMS production by cells.
[Mh] Termos MeSH primário: Sobrevivência Celular/efeitos dos fármacos
Dimetil Sulfóxido/farmacologia
Substâncias Protetoras/farmacologia
Compostos Orgânicos Voláteis/metabolismo
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/antagonistas & inibidores
Antineoplásicos Fitogênicos/toxicidade
Células Cultivadas
Células HEK293
Células HeLa
Seres Humanos
Paclitaxel/antagonistas & inibidores
Paclitaxel/toxicidade
Sulfonas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Protective Agents); 0 (Sulfones); 0 (Volatile Organic Compounds); 9H4PO4Z4FT (dimethyl sulfone); P88XT4IS4D (Paclitaxel); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180215
[St] Status:MEDLINE
[do] DOI:10.1691/ph.2016.6075


  4 / 14007 MEDLINE  
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[PMID]:29235601
[Au] Autor:Mandal P; Kundu BK; Vyas K; Sabu V; Helen A; Dhankhar SS; Nagaraja CM; Bhattacherjee D; Bhabak KP; Mukhopadhyay S
[Ad] Endereço:Department of Chemistry, School of Basic Sciences, Indian Institute of Technology Indore, Indore 453552, India. suman@iiti.ac.in.
[Ti] Título:Ruthenium(ii) arene NSAID complexes: inhibition of cyclooxygenase and antiproliferative activity against cancer cell lines.
[So] Source:Dalton Trans;47(2):517-527, 2018 Jan 02.
[Is] ISSN:1477-9234
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Non-steroidal anti-inflammatory drugs (NSAIDs) are a group of molecules which have been found to be active against cancer cells with chemopreventive properties by targeting cyclooxygenase (COX-1 and COX-2) and lipoxygenase (LOX), commonly upregulated (particularly COX-2) in malignant tumors. Arene ruthenium(ii) complexes with a pseudo-octahedral coordination environment containing different ancillary ligands have shown remarkable activity against primary and metastatic tumors as reported earlier. This work describes the synthesis of four novel ruthenium(ii)-arene complexes viz. [Ru(η -p-cymene)(nap)Cl] 1 [Hnap = naproxen or (S)-2-(6-methoxy-2-naphthyl)propionic acid], [Ru(η -p-cymene)(diclo)Cl] 2 [Hdiclo = diclofenac or 2-[(2,6-dichlorophenyl)amino] benzeneacetic acid, [Ru(η -p-cymene)(ibu)Cl] 3 [Hibu = ibuprofen or 2-(4-isobutylphenyl)propanoic acid] and [Ru(η -p-cymene)(asp)Cl] 4 [Hasp = aspirin or 2-acetoxy benzoic acid] using different NSAIDs as chelating ligands. Complexes 1-3 have shown promising antiproliferative activity against three different cell lines with GI (concentration of drug causing 50% inhibition of cell growth) values comparable to adriamycin. At the concentration of 50 µM, complex 3 is more effective in the inhibition of cyclooxygenase and lipooxygenase enzymes, followed by complex 2 and complex 1 in comparison to their respective free NSAID ligands indicating a possible correlation between the inhibition of COX and/or LOX and anticancer properties. Molecular docking studies with COX-2 reveal that complexes 1 and 2 having naproxen and diclofenac ligands exhibit stronger interactions with COX-2 than their respective free NSAIDs and these results are in good agreement with their relative experimentally observed COX inhibition as well as anti-proliferative activities.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/química
Benzeno/química
Compostos Organometálicos/química
Compostos Organometálicos/farmacologia
Prostaglandina-Endoperóxido Sintases/metabolismo
Rutênio/química
[Mh] Termos MeSH secundário: Animais
Bovinos
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Ciclo-Oxigenase 1/química
Ciclo-Oxigenase 1/metabolismo
Ciclo-Oxigenase 2/química
Ciclo-Oxigenase 2/metabolismo
Inibidores de Ciclo-Oxigenase/síntese química
Inibidores de Ciclo-Oxigenase/química
Inibidores de Ciclo-Oxigenase/metabolismo
Inibidores de Ciclo-Oxigenase/farmacologia
DNA/metabolismo
Dimetil Sulfóxido/química
Estabilidade de Medicamentos
Seres Humanos
Lipoxigenase/metabolismo
Inibidores de Lipoxigenase/síntese química
Inibidores de Lipoxigenase/química
Inibidores de Lipoxigenase/metabolismo
Inibidores de Lipoxigenase/farmacologia
Simulação de Acoplamento Molecular
Compostos Organometálicos/síntese química
Compostos Organometálicos/metabolismo
Prostaglandina-Endoperóxido Sintases/química
Conformação Proteica
Soroalbumina Bovina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Cyclooxygenase Inhibitors); 0 (Lipoxygenase Inhibitors); 0 (Organometallic Compounds); 27432CM55Q (Serum Albumin, Bovine); 7UI0TKC3U5 (Ruthenium); 9007-49-2 (DNA); EC 1.13.11.12 (Lipoxygenase); EC 1.14.99.1 (Cyclooxygenase 1); EC 1.14.99.1 (Cyclooxygenase 2); EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases); J64922108F (Benzene); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1039/c7dt03637j


  5 / 14007 MEDLINE  
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[PMID]:29304068
[Au] Autor:Drake AC; Lee Y; Burgess EM; Karlsson JOM; Eroglu A; Higgins AZ
[Ad] Endereço:School of Chemical, Biological and Environmental Engineering, Oregon State University, Corvallis, Oregon, United States of America.
[Ti] Título:Effect of water content on the glass transition temperature of mixtures of sugars, polymers, and penetrating cryoprotectants in physiological buffer.
[So] Source:PLoS One;13(1):e0190713, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Long-term storage of viable mammalian cells is important for applications ranging from in vitro fertilization to cell therapy. Cryopreservation is currently the most common approach, but storage in liquid nitrogen is relatively costly and the requirement for low temperatures during shipping is inconvenient. Desiccation is an alternative strategy with the potential to enable viable cell preservation at more convenient storage temperatures without the need for liquid nitrogen. To achieve stability during storage in the dried state it is necessary to remove enough water that the remaining matrix forms a non-crystalline glassy solid. Thus, the glass transition temperature is a key parameter for design of cell desiccation procedures. In this study, we have investigated the effects of moisture content on the glass transition temperature (Tg) of mixtures of sugars (trehalose or raffinose), polymers (polyvinylpyrrolidone or Ficoll), penetrating cryoprotectants (ethylene glycol, propylene glycol, or dimethyl sulfoxide), and phosphate buffered saline (PBS) solutes. Aqueous solutions were dried to different moisture contents by equilibration with saturated salt solutions, or by baking at 95°C. The glass transition temperatures of the dehydrated samples were then measured by differential scanning calorimetry. As expected, Tg increased with decreasing moisture content. For example, in a desiccation medium containing 0.1 M trehalose in PBS, Tg ranged from about 360 K for a completely dry sample to about 220 K at a water mass fraction of 0.4. Addition of polymers to the solutions increased Tg, while addition of penetrating cryoprotectants decreased Tg. Our results provide insight into the relationship between relative humidity, moisture content and glass transition temperature for cell desiccation solutions containing sugars, polymers and penetrating cryoprotectants.
[Mh] Termos MeSH primário: Crioprotetores/química
Polímeros/química
Açúcares/química
Temperatura de Transição
Água/química
[Mh] Termos MeSH secundário: Tampões (Química)
Varredura Diferencial de Calorimetria
Criopreservação/métodos
Dessecação/métodos
Dimetil Sulfóxido/química
Etilenoglicol/química
Ficoll/química
Vidro/química
Modelos Teóricos
Povidona/química
Propilenoglicol/química
Rafinose/química
Soluções/química
Trealose/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Buffers); 0 (Cryoprotective Agents); 0 (Polymers); 0 (Solutions); 0 (Sugars); 059QF0KO0R (Water); 25702-74-3 (Ficoll); 6DC9Q167V3 (Propylene Glycol); B8WCK70T7I (Trehalose); FC72KVT52F (Ethylene Glycol); FZ989GH94E (Povidone); N5O3QU595M (Raffinose); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190713


  6 / 14007 MEDLINE  
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[PMID]:28470628
[Au] Autor:Zhou Y; Liu K; Zhang J; Chu J; He B
[Ad] Endereço:College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, China.
[Ti] Título:Mg -induced stabilization of ß-galactosidase from Bacillus megaterium and its application in the galactosylation of natural products.
[So] Source:Biotechnol Lett;39(8):1175-1181, 2017 Aug.
[Is] ISSN:1573-6776
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To improve the stability of ß-galactosidase from Bacillus megaterium YZ08 (BMG) in aqueous hydrophilic solvents and promote its application in the galactosylation of natural products. RESULTS: The addition of 5 mM Mg significantly enhanced the stability of BMG in aqueous hydrophilic solvents, and the half-lives of BMG in these solutions reached 56 min to 208 h, while they were only 7 min to 5.9 h without addition of Mg . Studies on the kinetic parameters in buffer solution and 30% dimethyl sulfoxide (DMSO) indicated that the affinity of BMG to 2-nitrophenyl-ß-D-galactopyranoside and its catalytic efficiency (κ /K ) increased with the addition of Mg . Furthermore, the addition of Mg facilitated galactosylation reactions in 30% DMSO and increased product conversions by 24-41% due to the reversal of the thermodynamic equilibrium of hydrolysis. CONCLUSION: A convenient approach was established to improve the stability of BMG in aqueous hydrophilic solvents.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Produtos Biológicos/metabolismo
Magnésio/química
beta-Galactosidase/química
[Mh] Termos MeSH secundário: Bacillus megaterium
Proteínas de Bactérias/metabolismo
Produtos Biológicos/química
Dimetil Sulfóxido/química
Estabilidade Enzimática
Temperatura Alta
Fenômenos de Química Orgânica
beta-Galactosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Biological Products); EC 3.2.1.23 (beta-Galactosidase); I38ZP9992A (Magnesium); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180202
[Lr] Data última revisão:
180202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s10529-017-2344-z


  7 / 14007 MEDLINE  
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[PMID]:28470825
[Au] Autor:Börner T; Rämisch S; Bartsch S; Vogel A; Adlercreutz P; Grey C
[Ad] Endereço:Department of Chemistry and Biotechnology, Institute of Materials Science, Nestlé Research Center, Route du Jorat 57, 1000, Lausanne 26, Switzerland.
[Ti] Título:Three in One: Temperature, Solvent and Catalytic Stability by Engineering the Cofactor-Binding Element of Amine Transaminase.
[So] Source:Chembiochem;18(15):1482-1486, 2017 08 04.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Amine transaminase (ATA) catalyse enantioselectively the direct amination of ketones, but insufficient stability during catalysis limits their industrial applicability. Recently, we revealed that ATAs suffer from substrate-induced inactivation mechanism involving dissociation of the enzyme-cofactor intermediate. Here, we report on engineering the cofactor-ring-binding element, which also shapes the active-site entrance. Only two point mutations in this motif improved temperature and catalytic stability in both biphasic media and organic solvent. Thermodynamic analysis revealed a higher melting point for the enzyme-cofactor intermediate. The high cofactor affinity eliminates the need for pyridoxal 5'-phosphate supply, thus making large-scale reactions more cost effective. This is the first report on stabilising a tetrameric ATA by mutating a single structural element. As this structural "hotspot" is a common feature of other transaminases it could serve as a general engineering target.
[Mh] Termos MeSH primário: Transaminases/química
[Mh] Termos MeSH secundário: Sítios de Ligação
Dimetil Sulfóxido/química
Estabilidade Enzimática
Propilaminas/química
Engenharia de Proteínas
Estrutura Quaternária de Proteína
Fosfato de Piridoxal/química
Piridoxamina/análogos & derivados
Piridoxamina/química
Solventes/química
Temperatura Ambiente
Temperatura de Transição
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Propylamines); 0 (Solvents); 059QF0KO0R (Water); 5V5IOJ8338 (Pyridoxal Phosphate); 6466NM3W93 (Pyridoxamine); EC 2.6.1.- (Transaminases); P8W26T4MTD (2-propylamine); QWW7V29814 (pyridoxamine phosphate); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180122
[Lr] Data última revisão:
180122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201700236


  8 / 14007 MEDLINE  
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[PMID]:28465186
[Au] Autor:Marquez-Curtis LA; McGann LE; Elliott JAW
[Ad] Endereço:Department of Chemical and Materials Engineering, University of Alberta, Edmonton, Alberta, Canada; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada. Electronic address: marquezc@ualberta.ca.
[Ti] Título:Expansion and cryopreservation of porcine and human corneal endothelial cells.
[So] Source:Cryobiology;77:1-13, 2017 Aug.
[Is] ISSN:1090-2392
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Impairment of the corneal endothelium causes blindness that afflicts millions worldwide and constitutes the most often cited indication for corneal transplants. The scarcity of donor corneas has prompted the alternative use of tissue-engineered grafts which requires the ex vivo expansion and cryopreservation of corneal endothelial cells. The aims of this study are to culture and identify the conditions that will yield viable and functional corneal endothelial cells after cryopreservation. Previously, using human umbilical vein endothelial cells (HUVECs), we employed a systematic approach to optimize the post-thaw recovery of cells with high membrane integrity and functionality. Here, we investigated whether improved protocols for HUVECs translate to the cryopreservation of corneal endothelial cells, despite the differences in function and embryonic origin of these cell types. First, we isolated endothelial cells from pig corneas and then applied an interrupted slow cooling protocol in the presence of dimethyl sulfoxide (Me SO), with or without hydroxyethyl starch (HES). Next, we isolated and expanded endothelial cells from human corneas and applied the best protocol verified using porcine cells. We found that slow cooling at 1 °C/min in the presence of 5% Me SO and 6% HES, followed by rapid thawing after liquid nitrogen storage, yields membrane-intact cells that could form monolayers expressing the tight junction marker ZO-1 and cytoskeleton F-actin, and could form tubes in reconstituted basement membrane matrix. Thus, we show that a cryopreservation protocol optimized for HUVECs can be applied successfully to corneal endothelial cells, and this could provide a means to address the need for off-the-shelf cryopreserved cells for corneal tissue engineering and regenerative medicine.
[Mh] Termos MeSH primário: Criopreservação/métodos
Células Endoteliais
Epitélio Posterior/citologia
[Mh] Termos MeSH secundário: Actinas/metabolismo
Idoso
Animais
Crioprotetores/farmacologia
Dimetil Sulfóxido/farmacologia
Feminino
Seres Humanos
Derivados de Hidroxietil Amido/farmacologia
Masculino
Meia-Idade
Suínos
Proteína da Zônula de Oclusão-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Actins); 0 (Cryoprotective Agents); 0 (Hydroxyethyl Starch Derivatives); 0 (TJP1 protein, human); 0 (Zonula Occludens-1 Protein); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  9 / 14007 MEDLINE  
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[PMID]:28902853
[Au] Autor:Verbeurgt C; Veithen A; Carlot S; Tarabichi M; Dumont JE; Hassid S; Chatelain P
[Ad] Endereço:Department of Otorhinolaryngology, Erasme University Hospital, Free University of Brussels, Brussels, Belgium.
[Ti] Título:The human bitter taste receptor T2R38 is broadly tuned for bacterial compounds.
[So] Source:PLoS One;12(9):e0181302, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:T2R38 has been shown to be a specific bacterial detector implicated in innate immune defense mechanism of human upper airway. Several clinical studies have demonstrated that this receptor is associated with the development of chronic rhinosinusitis (CRS). T2R38 was previously reported to bind to homoserine lactones (HSL), quorum sensing molecules specific of Pseudomonas Aeruginosa and other gram negative species. Nevertheless, these bacteria are not the major pathogens found in CRS. Here we report on the identification of bacterial metabolites acting as new agonists of T2R38 based on a single cell calcium imaging study. Two quorum sensing molecules (Agr D1 thiolactone from Staphylococcus Aureus and CSP-1 from Streptococcus Pneumoniae) and a list of 32 bacterial metabolites from pathogens frequently implicated in CRS were tested. First, we observed that HSL failed to activate T2R38 in our experimental system, but that the dimethylsulfoxide (DMSO), used as a solvent for these lactones may, by itself, account for the agonistic effect previously described. Secondly, we showed that both Agr D1 thiolactone and CSP-1 are inactive but that at least 7 bacterial metabolites (acetone, 2-butanone, 2-pentanone, 2-methylpropanal, dimethyl disulfide, methylmercaptan, γ-butyrolactone) are able to specifically trigger this receptor. T2R38 is thus much more broadly tuned for bacterial compounds than previously thought.
[Mh] Termos MeSH primário: Antígenos de Bactérias/metabolismo
Imunidade Inata/genética
Receptores Acoplados a Proteínas-G/agonistas
Receptores Acoplados a Proteínas-G/fisiologia
[Mh] Termos MeSH secundário: 4-Butirolactona/análogos & derivados
4-Butirolactona/metabolismo
4-Butirolactona/farmacologia
Antígenos de Bactérias/imunologia
Doença Crônica
Dimetil Sulfóxido/metabolismo
Dimetil Sulfóxido/farmacologia
Células HEK293
Seres Humanos
Percepção de Quorum
Rinite/genética
Rinite/imunologia
Sinusite/genética
Sinusite/imunologia
Staphylococcus aureus/metabolismo
Streptococcus pneumoniae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Bacterial); 0 (N-butyrylhomoserine lactone); 0 (Receptors, G-Protein-Coupled); 0 (taste receptors, type 2); 76924-95-3 (N-(3-oxohexanoyl)-3-aminodihydro-2(3H)-furanone); OL659KIY4X (4-Butyrolactone); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181302


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[PMID]:28856935
[Au] Autor:Misuri L; Cappiello M; Balestri F; Moschini R; Barracco V; Mura U; Del-Corso A
[Ad] Endereço:a Department of Biology, Biochemistry Unit , University of Pisa , Pisa , Italy.
[Ti] Título:The use of dimethylsulfoxide as a solvent in enzyme inhibition studies: the case of aldose reductase.
[So] Source:J Enzyme Inhib Med Chem;32(1):1152-1158, 2017 Dec.
[Is] ISSN:1475-6374
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Aldose reductase (AR) is an enzyme devoted to cell detoxification and at the same time is strongly involved in the aetiology of secondary diabetic complications and the amplification of inflammatory phenomena. AR is subjected to intense inhibition studies and dimethyl sulfoxide (DMSO) is often present in the assay mixture to keep the inhibitors in solution. DMSO was revealed to act as a weak but well detectable AR differential inhibitor, acting as a competitive inhibitor of the L-idose reduction, as a mixed type of non-competitive inhibitor of HNE reduction and being inactive towards 3-glutathionyl-4-hydroxynonanal transformation. A kinetic model of DMSO action with respect to differently acting inhibitors was analysed. Three AR inhibitors, namely the flavonoids neohesperidin dihydrochalcone, rutin and phloretin, were used to evaluate the effects of DMSO on the inhibition studies on the reduction of L-idose and HNE.
[Mh] Termos MeSH primário: Aldeído Redutase/antagonistas & inibidores
Dimetil Sulfóxido/farmacologia
Inibidores Enzimáticos/farmacologia
[Mh] Termos MeSH secundário: Aldeído Redutase/isolamento & purificação
Aldeído Redutase/metabolismo
Dimetil Sulfóxido/síntese química
Dimetil Sulfóxido/química
Relação Dose-Resposta a Droga
Inibidores Enzimáticos/síntese química
Inibidores Enzimáticos/química
Seres Humanos
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/metabolismo
Solventes/síntese química
Solventes/química
Solventes/farmacologia
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Recombinant Proteins); 0 (Solvents); EC 1.1.1.21 (Aldehyde Reductase); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1080/14756366.2017.1363744



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