[PMID]: | 28506059 |
[Au] Autor: | Logue C; Dowey LRC; Strain JJ; Verhagen H; McClean S; Gallagher AM |
[Ad] Endereço: | Nutrition Innovation Centre for Food and Health (NICHE), Ulster University , Coleraine, Northern Ireland BT52 1SA. |
[Ti] Título: | Application of Liquid Chromatography-Tandem Mass Spectrometry To Determine Urinary Concentrations of Five Commonly Used Low-Calorie Sweeteners: A Novel Biomarker Approach for Assessing Recent Intakes? |
[So] Source: | J Agric Food Chem;65(22):4516-4525, 2017 Jun 07. |
[Is] ISSN: | 1520-5118 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | Although the use of low-calorie sweeteners (LCSs) is widespread, methods of assessing consumption within free-living populations have inherent limitations. Five commonly consumed LCSs, namely, acesulfame-K, saccharin, sucralose, cyclamate, and steviol glycosides, are excreted via the urine, and therefore a urinary biomarker approach may provide more objective LCS intake data. A LC-ESI-MS/MS method of simultaneously determining acesulfame-K, saccharin, sucralose, cyclamate, and the excretory metabolite of steviol glycosides, steviol glucuronide, in human urine was developed and validated. Linearity was observed over a concentration range of 10-1000 ng/mL with coefficients of determination ranging from 0.9969 to 0.9997. Accuracy ranged from 92 to 104%, and intrabatch and interday precisions were within acceptable limits with %CV below 8% for all compounds. A double-blind, randomized crossover dose-response study was conducted to assess the usefulness of urinary LCS excretions (from both fasting spot and a full 24-h urine collection) for investigating recent intakes. Both modes of sampling were useful for distinguishing between the three short-term intakes of acesulfame-K, saccharin, cyclamates, and steviol glycosides (p < 0.001), whereas for sucralose, urinary concentrations were useful for distinguishing between low (0.1% ADI) and high doses (10% ADI) only (p < 0.001). In summary, this biomarker approach may be useful for assessing intakes of five commonly consumed LCSs. |
[Mh] Termos MeSH primário: |
Biomarcadores/urina Cromatografia Líquida de Alta Pressão/métodos Edulcorantes/análise Espectrometria de Massas em Tandem/métodos Urina/química
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[Mh] Termos MeSH secundário: |
Biomarcadores/metabolismo Ciclamatos/análise Ciclamatos/metabolismo Diterpenos Caurânicos/metabolismo Diterpenos Caurânicos/urina Seres Humanos Sacarina/análise Sacarina/metabolismo Sacarose/análogos & derivados Sacarose/análise Sacarose/metabolismo Sacarose/urina Edulcorantes/metabolismo Tiazinas/metabolismo Tiazinas/urina
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[Pt] Tipo de publicação: | EVALUATION STUDIES; JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Biomarkers); 0 (Cyclamates); 0 (Diterpenes, Kaurane); 0 (Sweetening Agents); 0 (Thiazines); 4741LYX6RT (steviol); 57-50-1 (Sucrose); 96K6UQ3ZD4 (trichlorosucrose); FST467XS7D (Saccharin); MA3UYZ6K1H (acetosulfame) |
[Em] Mês de entrada: | 1706 |
[Cu] Atualização por classe: | 170619 |
[Lr] Data última revisão:
| 170619 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170517 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1021/acs.jafc.7b00404 |
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