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[PMID]:28177694
[Au] Autor:Demeter-Haludka V; Juhász L; Kovác M; Gardi J; Végh Á
[Ad] Endereço:a Department of Pharmacology and Pharmacotherapy, University of Szeged, Albert-Szent Györgyi Medical Centre, Szeged H-6720, Hungary.
[Ti] Título:Is there a role of inducible nitric oxide synthase activation in the delayed antiarrhythmic effect of sodium nitrite?
[So] Source:Can J Physiol Pharmacol;95(4):447-454, 2017 Apr.
[Is] ISSN:1205-7541
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:This study aimed to examine whether inducible nitric oxide synthase (iNOS) plays a role in the delayed antiarrhythmic effect of sodium nitrite. Twenty-one dogs were infused intravenously with sodium nitrite (0.2 µmol·kg ·min ) for 20 min, either in the absence (n = 12) or in the presence of the iNOS inhibitor S-(2-aminoethyl)-isothiourea (AEST) (total dose 2.0 mg·kg i.v., n = 9). Control dogs (n = 12) were given saline. Twenty-four hours later, all of the dogs were subjected to a 25 min period occlusion of the left anterior descending coronary artery followed by rapid reperfusion. Dogs treated with AEST and nitrite received again AEST prior to the occlusion. Compared with the controls, sodium nitrite markedly reduced the number of ectopic beats, the number and incidence of ventricular tachycardia, and the incidence of ventricular fibrillation during occlusion and increased survival (0% versus 50%) from the combined ischaemia and reperfusion insult. Although AEST completely inhibited iNOS activity, the nitrite-induced increase in NO bioavailability during occlusion was not substantially modified. Furthermore, AEST attenuated but did not completely abolish the antiarrhythmic effect of nitrite. The marked delayed antiarrhythmic effect of sodium nitrite is not entirely due to the activation of iNOS; other mechanisms may certainly play a role.
[Mh] Termos MeSH primário: Antiarrítmicos/farmacologia
Arritmias Cardíacas/prevenção & controle
Hemodinâmica/efeitos dos fármacos
Traumatismo por Reperfusão Miocárdica/complicações
Óxido Nítrico Sintase Tipo II/metabolismo
Nitrito de Sódio/farmacologia
[Mh] Termos MeSH secundário: Animais
Antiarrítmicos/administração & dosagem
Antiarrítmicos/uso terapêutico
Arritmias Cardíacas/etiologia
Cães
Precondicionamento Isquêmico Miocárdico/métodos
Traumatismo por Reperfusão Miocárdica/etiologia
Óxido Nítrico/metabolismo
Óxido Nítrico Sintase Tipo II/antagonistas & inibidores
Nitrito de Sódio/administração & dosagem
Nitrito de Sódio/uso terapêutico
Taquicardia Ventricular/etiologia
Taquicardia Ventricular/prevenção & controle
beta-Aminoetil Isotioureia/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Arrhythmia Agents); 151-16-6 (beta-Aminoethyl Isothiourea); 31C4KY9ESH (Nitric Oxide); EC 1.14.13.39 (Nitric Oxide Synthase Type II); M0KG633D4F (Sodium Nitrite)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.1139/cjpp-2016-0357


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[PMID]:26836511
[Au] Autor:Kanof ME
[Ad] Endereço:Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
[Ti] Título:Isolation of T Cells Using Rosetting Procedures.
[So] Source:Curr Protoc Immunol;112:7.2.1-5, 2016 Feb 02.
[Is] ISSN:1934-368X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This unit describes a procedure for separating T cells from other mononuclear cells by exploiting the unique ability of cells to bind to and form rosettes with sheep red blood cells (SRBC). This isolation method also allows recovery of the nonrosetting cell population (B lymphocytes, monocytes, and macrophages). Neuraminidase- and 2-aminoethylisothiouronium bromide (AET)-treated SRBC are used for rosetting because of enhanced binding to T cells. It should be noted that use of the rosetting technique to obtain purified T cells or purified non-T cells by negative selection has largely been superceded by other techniques such as panning and immunomagnetic beads.
[Mh] Termos MeSH primário: Eritrócitos/imunologia
Linfócitos T/imunologia
[Mh] Termos MeSH secundário: Animais
Separação Celular
Eritrócitos/efeitos dos fármacos
Seres Humanos
Leucócitos Mononucleares/citologia
Neuraminidase/farmacologia
Formação de Roseta
Ovinos
beta-Aminoetil Isotioureia/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
151-16-6 (beta-Aminoethyl Isothiourea); EC 3.2.1.18 (Neuraminidase)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160203
[St] Status:MEDLINE
[do] DOI:10.1002/0471142735.im0702s112


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[PMID]:23516890
[Au] Autor:Filimonova MV; Proskuriakov SIa; Shevchenko LI; Shevchuk AS; Lushnikova GA; Makarchuk VM; Arzamastsev EV; Laba VI; Malinovskaia KI; Levitskaia EL
[Ti] Título:[Radioprotective properties of isothiourea derivatives with NO-inhibitory mechanism of action].
[So] Source:Radiats Biol Radioecol;52(6):593-601, 2012 Nov-Dec.
[Is] ISSN:0869-8031
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The study of the radioprotective activity of S-[2-alkyl (aryl) sulfonyl]-S-ethyl derivatives of (vinyl)-isothiourea in (he model of the survival of mice exposed to gamma-radiation at a dose of 10 Gy has shown that the incorporation of additional sulfur-containing groups does not increase the radioprotective properties of compounds. In contrast to aminoalkil thiols, the effectiveness of the radiation protection action of the isothiourea (ITU) derivatives studied clearly correlates with the NO-inhibitory activity. This fact allowed us to assume that the radioprotective effect of S-substituted ITU caused inhibition of the endogenous synthesis of NO, which promotes the development of circulatory hypoxia, and that a further search for the radioprotective agents in this class of chemicals should be considered as the search for effective inhibitors of NO-synthase (NOS). The theoretical analysis of the conformity of molecular structures to the composition and topology of the active center of NOS-inhibitors allowed us to prognosticate a number of new ITU derivatives with the potential NOS-inhibiting ability. As a result of further theoretical and experimental studies, four S,N-disubstituted ITU derivatives - active non-selective NOS-inhibitors, were first identified and synthesized. These compounds exhibited a pronounced and prolonged vasopressive effects at doses of 0.01-0.05 LD50/15 in the models of severe hemorrhagic and endotoxic shock, and provided 65-100% 30-day survival at doses of 0.2-0.3 LD50/15 in the mice irradiated by gamma-rays at a dose of 10 Gy (LD98/30).The findings suggest the pronounced radioprotective effect of NOS-inhibitors among the ITU-derivatives.
[Mh] Termos MeSH primário: Óxido Nítrico Sintase
Protetores contra Radiação/administração & dosagem
beta-Aminoetil Isotioureia
[Mh] Termos MeSH secundário: Animais
Inibidores Enzimáticos/administração & dosagem
Raios gama
Dose Letal Mediana
Camundongos
Óxido Nítrico Sintase/antagonistas & inibidores
Óxido Nítrico Sintase/metabolismo
Protetores contra Radiação/síntese química
beta-Aminoetil Isotioureia/administração & dosagem
beta-Aminoetil Isotioureia/análogos & derivados
beta-Aminoetil Isotioureia/síntese química
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Radiation-Protective Agents); 151-16-6 (beta-Aminoethyl Isothiourea); EC 1.14.13.39 (Nitric Oxide Synthase)
[Em] Mês de entrada:1304
[Cu] Atualização por classe:161020
[Lr] Data última revisão:
161020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130323
[St] Status:MEDLINE


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[PMID]:21118267
[Au] Autor:des Roziers NB; Bodivit G; Chadebech P; Nzouakou R; Bierling P; Noizat-Pirenne F
[Ad] Endereço:Etablissement Français du Sang Ile de France, Cochin Hospital, 33 rue du Faubourg Saint-Jacques, Paris, France. nicolas.burin-des-roziers@efs.sante.fr
[Ti] Título:Anti-T haemolysins: the effects of sialic acid removal and 2-aminoethylisothiouronium bromide treatment of erythrocytes on immune lysis.
[So] Source:Vox Sang;100(4):401-8, 2011 May.
[Is] ISSN:1423-0410
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND OBJECTIVES: Intravascular haemolytic reactions are reported in red-cell T-activated patients after blood transfusion. The relationship between T antigen antibodies present in normal plasma and these reactions remains unclear. In this study, we assessed the haemolytic activity of T antibodies in vitro in comparison with anti-A/B antibodies. MATERIALS AND METHODS: We established a haemolysis assay based on treating target red-blood-cells (RBCs) with 2-aminoethylisothiouronium bromide (AET). Two hundred and seven blood donor sera were analysed for anti-T, anti-A/B haemolysins and anti-T agglutinins. RESULTS: Anti-T haemolysins were found in 4 (1·9%) blood donor sera using a standard haemolysis method and in 174 (84%) samples using AET-treated RBCs. Haemolysis correlated with agglutination titres (P<10(-7) ). With both methods, anti-T haemolysins were much weaker than anti-A and anti-B haemolysins. Gradual desialylation of RBCs showed a correlation between sialic acid level as indicated by agglutination with Sambucus nigra lectin and anti-T mediated haemolysis that was significantly increased (fold 2·4) independently of T antigen expression. CONCLUSION: These data indicate that, in vitro, anti-T-mediated haemolysis depends primarily on the degree of desialylation of target RBCs. They suggest that the haemolytic activity of T antibodies-containing human sera is usually weak and may only become significant in the very rare setting of a profound desialylation of RBCs.
[Mh] Termos MeSH primário: Antígenos Glicosídicos Associados a Tumores/imunologia
Incompatibilidade de Grupos Sanguíneos/imunologia
Eritrócitos/imunologia
Hemólise
Isoanticorpos/imunologia
Ácido N-Acetilneuramínico
Protetores contra Radiação/farmacologia
beta-Aminoetil Isotioureia/farmacologia
[Mh] Termos MeSH secundário: Antígenos Glicosídicos Associados a Tumores/sangue
Incompatibilidade de Grupos Sanguíneos/sangue
Eritrócitos/metabolismo
Feminino
Hemólise/efeitos dos fármacos
Hemólise/imunologia
Seres Humanos
Isoanticorpos/sangue
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Tumor-Associated, Carbohydrate); 0 (Isoantibodies); 0 (Radiation-Protective Agents); 151-16-6 (beta-Aminoethyl Isothiourea); 3554-90-3 (Thomsen-Friedenreich antigen); GZP2782OP0 (N-Acetylneuraminic Acid)
[Em] Mês de entrada:1110
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:101202
[St] Status:MEDLINE
[do] DOI:10.1111/j.1423-0410.2010.01450.x


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[PMID]:19763898
[Au] Autor:Li SB; Nie HL; Zhang HT; Xue Y; Zhu LM
[Ad] Endereço:College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
[Ti] Título:Kinetic evaluation of aminoethylisothiourea on mushroom tyrosinase activity.
[So] Source:Appl Biochem Biotechnol;162(3):641-53, 2010 Oct.
[Is] ISSN:1559-0291
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study demonstrates that aminoethylisothiourea (AET), a potent inhibitor of inducible nitric oxide synthase, is an irreversible competitive inhibitor of mushroom tyrosinase by chelation to the active site of tyrosinase when L-3,4-dihydroxyphenylalanine was assayed spectrophotometrically. The spectrophotometric recordings of the inhibition of tyrosinase by AET were characterized by the presence of a lag period prior to the attainment of an inhibited steady-state rate. The lag period corresponded to the time in which AET was reacting with the enzymatically generated o-quinone. Increasing AET concentrations provoked longer lag periods as well as a concomitant decrease in the tyrosinase activity. Both lag period and steady-state rate were dependent on AET, substrate, and tyrosinase concentrations. The inhibition of diphenolase activity of tyrosinase by AET showed positive kinetic cooperativity which arose from the protection of both substrate and o-quinone against inhibition by AET. The UV-visible spectrum of a mixture of tyrosinase and AET exhibited a characteristic shoulder peak ascribed to the chelation of AET to the active site of tyrosinase. Moreover, the presence of copper ions only partially prevented but not reverted mushroom tyrosinase inhibition when CuSO(4) was added to the assay medium on tyrosinase activity.
[Mh] Termos MeSH primário: Agaricales/enzimologia
Inibidores Enzimáticos/farmacologia
Monofenol Mono-Oxigenase/metabolismo
beta-Aminoetil Isotioureia/farmacologia
[Mh] Termos MeSH secundário: Ativação Enzimática/efeitos dos fármacos
Cinética
Modelos Químicos
Quinonas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Quinones); 151-16-6 (beta-Aminoethyl Isothiourea); EC 1.14.18.1 (Monophenol Monooxygenase)
[Em] Mês de entrada:1008
[Cu] Atualização por classe:100531
[Lr] Data última revisão:
100531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090919
[St] Status:MEDLINE
[do] DOI:10.1007/s12010-009-8760-3


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[PMID]:19581205
[Au] Autor:Benkovic V; Knezevic AH; Dikic D; Lisicic D; Orsolic N; Basic I; Kopjar N
[Ad] Endereço:Department of Animal Physiology, Faculty of Science, University of Zagreb, Zagreb, Croatia. vesna@pmf.biol.hr
[Ti] Título:Radioprotective effects of quercetin and ethanolic extract of propolis in gamma-irradiated mice.
[So] Source:Arh Hig Rada Toksikol;60(2):129-38, 2009 Jun.
[Is] ISSN:1848-6312
[Cp] País de publicação:Croatia
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to assess radioprotective effects of quercetin and the ethanolic extract of propolis (EEP) in CBA mice exposed to a single radiation dose 4 Gy (60Co). The mice were treated with 100 mg kg(-1) quercetin or EEP a day for three consecutive days either before (pre-treatment) or after gamma-irradiation (therapy). Leukocyte count was determined in blood drawn from the tail vein, and DNA damage in leukocytes was assessed using the alkaline comet assay. Genotoxic effects of the test compounds were also evaluated in non-irradiated mice. The levels of radioprotection provided by both test compounds were compared with those established in mice that were given chemical radioprotector S-(2-aminoethy1)isothiouronium bromide hydrobromide (AET). Mice that received pre-treatment were less sensitive to irradiation. Mice given the post-irradiation therapy showed a slight but not significant increase in total leukocyte count over irradiated negative control. Quercetin showed better protective properties than EEP in both pre-treatment and therapy, and activated a higher number of leukocytes in non-irradiated mice. The alkaline comet assay suggests that both natural compounds, especially when given as pre-treatment, protect against primary leukocyte DNA damage in mice. At tested concentrations, EEP and quercetin were not genotoxic to non-irradiated mice. AET, however, caused a slight but not significant increase in DNA damage. Although the results of this study show the radioprotective potential of the test compounds, further investigation is needed to clarify the underlying protection mechanisms.
[Mh] Termos MeSH primário: Própole/farmacologia
Quercetina/farmacologia
Lesões Experimentais por Radiação/prevenção & controle
Protetores contra Radiação/farmacologia
[Mh] Termos MeSH secundário: Animais
Ensaio Cometa
Dano ao DNA/efeitos da radiação
Raios gama
Contagem de Leucócitos
Masculino
Camundongos
Camundongos Endogâmicos CBA
beta-Aminoetil Isotioureia/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Radiation-Protective Agents); 151-16-6 (beta-Aminoethyl Isothiourea); 9009-62-5 (Propolis); 9IKM0I5T1E (Quercetin)
[Em] Mês de entrada:0912
[Cu] Atualização por classe:161205
[Lr] Data última revisão:
161205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090708
[St] Status:MEDLINE
[do] DOI:10.2478/10004-1254-60-2009-1908


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[PMID]:18239798
[Au] Autor:Malysheva EV; Kruglov SV; Nazarov VA; Manukhina EB; Malyshev IY
[Ad] Endereço:Moscow State Medical Stomatological University.
[Ti] Título:Intracellular and extracellular NO differentially modulates the stress response and apoptosis in macrophages exposed to the influence of biological or physical factors.
[So] Source:Bull Exp Biol Med;143(6):673-7, 2007 Jun.
[Is] ISSN:0007-4888
[Cp] País de publicação:United States
[La] Idioma:eng; rus
[Ab] Resumo:We studied the role of extracellular and intracellular NO in the regulation of the stress response and apoptosis in macrophages of proinflammatory and antiinflammatory phenotypes under the influence of S. aureus and heat shock. Blockade of extracellular nitric oxide synthesis in cells with antiinflammatory phenotype inhibited the stress response induced by S. aureus and heat shock. The decrease in extracellular nitric oxide concentration around antiinflammatory macrophages potentiated the stress response induced by S. aureus, but had no effect on the stress response induced by heat shock. Hence, intracellular NO mediates the stress response induced by S. aureus and heat shock, while extracellular NO inhibits the stress response induced by S. aureus, but has no effect on the stress response induced by heat shock. In cells with antiinflammatory phenotype, intracellular NO plays an antiapoptotic role. S. aureus and heat shock did not cause apoptosis in macrophages with proinflammatory phenotype, while intracellular NO did not play a role in antiapoptotic activity of the proinflammatory phenotype. Extracellular NO synthesized by macrophages protects these cells from apoptosis induced by S. aureus and heat shock.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Proteínas de Choque Térmico HSP70/biossíntese
Macrófagos/fisiologia
Óxido Nítrico/fisiologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Fragmentação do DNA
Inflamação/induzido quimicamente
Lipopolissacarídeos
Macrófagos/citologia
Macrófagos/efeitos dos fármacos
Camundongos
Staphylococcus aureus/imunologia
beta-Aminoetil Isotioureia/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (HSP70 Heat-Shock Proteins); 0 (Lipopolysaccharides); 151-16-6 (beta-Aminoethyl Isothiourea); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:0802
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080202
[St] Status:MEDLINE


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[PMID]:17541161
[Au] Autor:Ohta S; Matsuda S; Gunji M; Kamogawa A
[Ad] Endereço:Department of Biology, Faculty of Pharmaceutical Sciences, Hoshi University, Tokyo, Japan. ohta@hoshi.ac.jp
[Ti] Título:The role of nitric oxide in radiation damage.
[So] Source:Biol Pharm Bull;30(6):1102-7, 2007 Jun.
[Is] ISSN:0918-6158
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:This study investigated the role of nitric oxide in radiation-induced damage by examining changes in mouse serum nitrate concentrations after irradiation. In addition, the contribution of S-2-aminoethylisothiourea 2HBr (AET) to the mechanisms of radiation damage protection was also clarified. The serum nitrate concentration increased as soon as 1.5 h after irradiation, and after 2.5 to 3.0 h the concentrations were significantly higher compared with normal levels. Normal levels were re-established after 12 h. Post-irradiation serum nitrate concentrations increased dose-dependently with irradiation dose (19.6-31.5 Gy). AET suppressed increases in the serum nitrate concentration following irradiation while 2-mercaptoethylamine HCl (MEA) did not. AET has an inhibitory effect on inducible nitric oxide synthase (iNOS); therefore, the increase in nitric oxide after irradiation may be produced by iNOS. Combined administration of irradiation and lipopolysaccharide (LPS) induced a significant increase in serum nitrate concentration, and a significant decrease in survival rate, compared with irradiation alone. The administration of AET or aminoguanidine increased survival rate following irradiation. In contrast to findings after LPS administration, IL-1beta and IFN-gamma were not determined in serum following irradiation. Existing iNOS is activated by irradiation, and nitric oxide production appears to increase without iNOS induction. Thus, the irradiation-induced increase in nitric oxide may be related to lethal injury.
[Mh] Termos MeSH primário: Nitratos/efeitos da radiação
Óxido Nítrico/metabolismo
Irradiação Corporal Total
[Mh] Termos MeSH secundário: Animais
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta à Radiação
Guanidinas/farmacologia
Lipopolissacarídeos/toxicidade
Masculino
Camundongos
Camundongos Endogâmicos ICR
Nitratos/sangue
Óxido Nítrico Sintase Tipo II/antagonistas & inibidores
Óxido Nítrico Sintase Tipo II/genética
Óxido Nítrico Sintase Tipo II/efeitos da radiação
Dose de Radiação
Protetores contra Radiação/farmacologia
Fatores de Tempo
beta-Aminoetil Isotioureia/farmacologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Guanidines); 0 (Lipopolysaccharides); 0 (Nitrates); 0 (Radiation-Protective Agents); 151-16-6 (beta-Aminoethyl Isothiourea); 31C4KY9ESH (Nitric Oxide); EC 1.14.13.39 (Nitric Oxide Synthase Type II); SCQ4EZQ113 (pimagedine)
[Em] Mês de entrada:0707
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070602
[St] Status:MEDLINE


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[PMID]:16415068
[Au] Autor:Tun NN; Santa-Catarina C; Begum T; Silveira V; Handro W; Floh EI; Scherer GF
[Ad] Endereço:Universität Hannover, Institut für Zierpflanzenbau, AG Molekulare Ertragsphysiologie, Germany.
[Ti] Título:Polyamines induce rapid biosynthesis of nitric oxide (NO) in Arabidopsis thaliana seedlings.
[So] Source:Plant Cell Physiol;47(3):346-54, 2006 Mar.
[Is] ISSN:0032-0781
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:In this study, we examined the regulation by putrescine, spermidine and spermine of nitric oxide (NO) biosynthesis in Arabidopsis thaliana seedlings. Using a fluorimetric method employing the cell-impermeable NO-binding dye diaminorhodamine-4M (DAR-4M), we observed that the polyamines (PAs) spermidine and spermine greatly increased NO release in the seedlings, whereas arginine and putrescine had little or no effect. Spermine, the most active PA, stimulated NO release with no apparent lag phase. The response was quenched by addition of 2-aminoethyl-2-thiopseudourea (AET), an inhibitor of the animal nitric oxide synthase (NOS) and plant NO biosynthesis, and by 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-1-oxy-3-oxide (PTIO), an NO scavenger. By fluorescence microscopy, using the cell-permeable NO-binding dye diaminorhodamine-4M acetoxymethyl ester (DAR-4M AM), we observed that PAs induced NO biosynthesis in specific tissues in Arabidopsis seedlings. Spermine and spermidine increased NO biosynthesis in the elongation zone of the Arabidopsis root tip and in primary leaves, especially in the veins and trichomes, while in cotyledons little or no effect of PAs beyond the endogenous levels of NO-induced fluorescence was observed. We conclude that PAs induce NO biosynthesis in plants.
[Mh] Termos MeSH primário: Arabidopsis/efeitos dos fármacos
Arabidopsis/metabolismo
Óxido Nítrico/biossíntese
Poliaminas/farmacologia
Plântulas/efeitos dos fármacos
Plântulas/metabolismo
[Mh] Termos MeSH secundário: Arginina/farmacologia
Óxidos N-Cíclicos/farmacologia
Fluorescência
Imidazóis/farmacologia
Microscopia de Fluorescência
Óxido Nítrico/antagonistas & inibidores
Óxido Nítrico/secreção
Folhas de Planta/citologia
Folhas de Planta/efeitos dos fármacos
Putrescina/farmacologia
Rodaminas/farmacologia
Espermina/farmacologia
beta-Aminoetil Isotioureia/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cyclic N-Oxides); 0 (Imidazoles); 0 (Polyamines); 0 (Rhodamines); 0 (diaminorhodamine-4M); 151-16-6 (beta-Aminoethyl Isothiourea); 18390-00-6 (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide); 2FZ7Y3VOQX (Spermine); 31C4KY9ESH (Nitric Oxide); 94ZLA3W45F (Arginine); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:0607
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060118
[St] Status:MEDLINE


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[PMID]:15907332
[Au] Autor:Hajnal A; Nagy O; Litvai A; Papp J; Parratt JR; Végh A
[Ad] Endereço:Department of Pharmacology and Pharmacotherapy, University of Szeged, Albert Szent-Györgyi Faculty of Medicine, Dóm tér 12, P.O. Box 427, H-6701 Hungary.
[Ti] Título:Nitric oxide involvement in the delayed antiarrhythmic effect of treadmill exercise in dogs.
[So] Source:Life Sci;77(16):1960-71, 2005 Sep 02.
[Is] ISSN:0024-3205
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:We have shown previously that a single period of treadmill exercise in dogs protects the heart against the severe ventricular arrhythmias that arise when a major (anterior descending) branch of the left coronary artery is occluded following anaesthesia 24 h later. This protection is aminoguanidine sensitive, suggesting a role for nitric oxide (NO) in this exercise-induced delayed antiarrhythmic effect. The present study has further examined the possible role of NO as a mediator and/or as a trigger using the selective induced (iNOS) inhibitor S-(2-aminoethyl)-methyl-isothiourea (AEST) and the specific but not selective nitric oxide synthase inhibitor N(omega)-nitro-L-arginine-methyl-ester (L-NAME). Exercise markedly reduced the severity of ischaemia and reperfusion-induced ventricular arrhythmias 24 h later. Thus, only one of the dogs (8%) so exercised fibrillated on occlusion (contrast 46% in the control, non-exercised dogs; P<0.05) and the marked changes in the inhomogeneity of electrical activation that occur in the ischaemic region following occlusion were much reduced (P<0.05 compared to controls). This delayed exercise-induced cardioprotection was significantly attenuated by the nitric oxide synthase (NOS) inhibitors L-NAME, given prior to the exercise protocol and by AEST given prior to the coronary artery occlusion. For example, survival from the ischaemia-reperfusion insult was 54% in the exercise dogs, 0% in the controls and 14% in those dogs given a NOS inhibitor. We conclude that nitric oxide (NO) is both the trigger and the mediator of this delayed protection against ischaemia and reperfusion-induced arrhythmias.
[Mh] Termos MeSH primário: Doenças do Cão/metabolismo
Doenças do Cão/prevenção & controle
Óxido Nítrico/metabolismo
Condicionamento Físico Animal/fisiologia
Traumatismo por Reperfusão/veterinária
Fibrilação Ventricular/veterinária
[Mh] Termos MeSH secundário: Análise de Variância
Animais
Cães
Hemodinâmica/efeitos dos fármacos
NG-Nitroarginina Metil Éster/farmacologia
Óxido Nítrico Sintase/antagonistas & inibidores
Óxido Nítrico Sintase Tipo II
Traumatismo por Reperfusão/complicações
Traumatismo por Reperfusão/metabolismo
Fibrilação Ventricular/etiologia
Fibrilação Ventricular/metabolismo
Fibrilação Ventricular/prevenção & controle
beta-Aminoetil Isotioureia/farmacologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
151-16-6 (beta-Aminoethyl Isothiourea); 31C4KY9ESH (Nitric Oxide); EC 1.14.13.39 (Nitric Oxide Synthase); EC 1.14.13.39 (Nitric Oxide Synthase Type II); V55S2QJN2X (NG-Nitroarginine Methyl Ester)
[Em] Mês de entrada:0510
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:050524
[St] Status:MEDLINE



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