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[PMID]:28240785
[Au] Autor:Legendre KP; Leissinger M; Le Donne V; Grasperge BJ; Gaunt SD
[Ad] Endereço:Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, USA.
[Ti] Título:The effect of urea on refractometric total protein measurement in dogs and cats with azotemia.
[So] Source:Vet Clin Pathol;46(1):138-142, 2017 Mar.
[Is] ISSN:1939-165X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: While protein is the predominant solute measured in plasma or serum by a refractometer, nonprotein substances also contribute to the angle of refraction. There is debate in the current literature regarding which nonprotein substances cause factitiously high refractometric total protein measurements, as compared to the biuret assay. OBJECTIVES: The purpose of the study was to determine if the blood of azotemic animals, specifically with increased blood urea concentration, will have significantly higher refractometric total protein concentrations compared to the total protein concentrations measured by biuret assay. METHODS: A prospective case series was conducted by collecting data from azotemic (n = 26) and nonazotemic (n = 34) dogs and cats. In addition, an in vitro study was performed where urea was added to an enhanced electrolyte solution at increasing concentrations, and total protein was assessed by both the refractometer and spectrophotometer. Statistical analysis was performed to determine the effect of urea. RESULTS: The refractometric total protein measurement showed a positive bias when compared to the biuret protein measurement in both groups, but the bias was higher in the azotemic group vs the nonazotemic group. The mean difference in total protein measurements of the nonazotemic group (0.59 g/dL) was significantly less (P < .01) than the mean difference of the azotemic group (0.95 g/dL). The in vitro experiment revealed a positive bias with a proportional error. CONCLUSIONS: This study demonstrated that increasing concentrations of urea significantly increased the total protein concentration measured by the refractometer as compared to the biuret assay, both in vivo and in vitro.
[Mh] Termos MeSH primário: Azotemia/veterinária
Doenças do Gato/sangue
Doenças do Cão/sangue
Ureia/sangue
[Mh] Termos MeSH secundário: Animais
Azotemia/sangue
Bilirrubina/sangue
Biureto
Proteínas Sanguíneas/análise
Gatos
Cães
Estudos Prospectivos
Refratometria/veterinária
Espectrofotometria/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Proteins); 89LJ369D1H (Biuret); 8W8T17847W (Urea); RFM9X3LJ49 (Bilirubin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE
[do] DOI:10.1111/vcp.12464


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[PMID]:28159268
[Au] Autor:Liu Z; Pan J
[Ad] Endereço:COFCO Nutrition and Health Research Institute, Beijing 102209, China. Electronic address: liuzelong@cofco.com.
[Ti] Título:A practical method for extending the biuret assay to protein determination of corn-based products.
[So] Source:Food Chem;224:289-293, 2017 Jun 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A modified biuret method suitable for protein determination of corn-based products was developed by introducing a combination of an alkaline reagent with sodium dodecyl sulfate (reagent A) and heat treatments. The method was tested on seven corn-based samples. The results showed mostly good agreement (P>0.05) as compared to the Kjeldahl values. The proposed method was found to enhance the accuracy of prediction on zein content using bovine serum albumin as standard. Reagent A and sample treatment were proved to effectively improve protein solubilization for the thermally-dried corn-based products, e.g. corn gluten meal. The absorbance was stable for at least 1-h. Moreover, the whole measurement of protein content only needs 15-20min more than the traditional biuret assay, and can be performed in batches. The findings suggest that the proposed method could be a timesaving alternative for routine protein analyses in corn processing factories.
[Mh] Termos MeSH primário: Biureto
Colorimetria/métodos
Proteínas de Plantas/análise
Zea mays/química
[Mh] Termos MeSH secundário: Manipulação de Alimentos/métodos
Glutens
Temperatura Alta
Indicadores e Reagentes
Sementes/química
Soroalbumina Bovina
Solubilidade
Zeína/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indicators and Reagents); 0 (Plant Proteins); 27432CM55Q (Serum Albumin, Bovine); 8002-80-0 (Glutens); 89LJ369D1H (Biuret); 9010-66-6 (Zein)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE


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[PMID]:26479154
[Au] Autor:Dutta A; Vasudevan V; Nain L; Singh N
[Ad] Endereço:a Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute , New Delhi , India.
[Ti] Título:Characterization of bacterial diversity in an atrazine degrading enrichment culture and degradation of atrazine, cyanuric acid and biuret in industrial wastewater.
[So] Source:J Environ Sci Health B;51(1):24-34, 2016.
[Is] ISSN:1532-4109
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An enrichment culture was used to study atrazine degradation in mineral salt medium (MSM) (T1), MSM+soil extract (1:1, v/v) (T2) and soil extract (T3). Results suggested that enrichment culture required soil extract to degrade atrazine, as after second sequential transfer only partial atrazine degradation was observed in T1 treatment while atrazine was completely degraded in T2 and T3 treatments even after fourth transfer. Culture independent polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique confirmed selective enrichment of genus Bacillus along with Pseudomonas and Burkholderia. Degradation of atrazine/metabolites in the industrial wastewater was studied at different initial concentrations of the contaminants [wastewater-water (v/v) ratio: T1, 1:9; T2, 2:8; T3, 3:7; T4, 5:5 and T5, undiluted effluent]. The initial concentrations of atrazine, cyanuric acid and biuret ranged between 5.32 and 53.92 µg mL(-1), 265.6 and 1805.2 µg mL(-1) and 1.85 and 16.12 µg mL(-1), respectively. The enrichment culture was able to completely degrade atrazine, cyanuric acid and biuret up to T4 treatment, while no appreciable degradation of contaminants was observed in the undiluted effluent (T5). Inability of enrichment culture to degrade atrazine/metabolites might be due to high concentrations of cyanuric acid. Therefore, a separate study on cyanuric acid degradation suggested: (i) no appreciable cyanuric acid degradation with accumulation of an unidentified metabolite in the medium where cyanuric acid was supplemented as the sole source of carbon and nitrogen; (ii) partial cyanuric acid degradation with accumulation of unidentified metabolite in the medium containing additional nitrogen source; and (iii) complete cyanuric acid degradation in the medium supplemented with an additional carbon source. This unidentified metabolite observed during cyanuric acid degradation and also detected in the enrichment culture inoculated wastewater samples, however, was degraded up to T4 treatments and was persistent in the T5 treatment. Probably, accumulation of this metabolite inhibited atrazine/cyanuric acid degradation by the enrichment culture in undiluted wastewater.
[Mh] Termos MeSH primário: Atrazina/metabolismo
Bactérias/metabolismo
Biureto/metabolismo
Triazinas/metabolismo
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Bactérias/classificação
Cromatografia Líquida de Alta Pressão
Eletroforese em Gel de Gradiente Desnaturante
Microbiota
Reação em Cadeia da Polimerase
Águas Residuais/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Triazines); 0 (Waste Water); 0 (Water Pollutants, Chemical); 89LJ369D1H (Biuret); H497R4QKTZ (cyanuric acid); QJA9M5H4IM (Atrazine)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:151120
[Lr] Data última revisão:
151120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151020
[St] Status:MEDLINE
[do] DOI:10.1080/03601234.2015.1080487


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[PMID]:25980141
[Au] Autor:Chen N; Zheng S
[Ti] Título:[Application research of protein test by using biuret reagent].
[So] Source:Zhongguo Yi Liao Qi Xie Za Zhi;38(6):458-60, 2014 Nov.
[Is] ISSN:1671-7104
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To investigate the biuret reagent to detect proteins in the application, the impact of different test conditions for test results. METHODS: The biuret method to select three different instruments, reagents, calibrators are arranged in combination to form 27 sets of detection systems, each detection system is a combination of 5 serum samples for testing, 5 measured values obtained, the selection process normality good a serum for the study to determine the mean value of all AST after culling outliers obtained in order to calculate the various detection systems use a combination of biuret reagent to detect proteins bias. RESULTS: The use of different detection equipment to detect proteins biuret reagent bias, homogeneity of variance (P = 0.467), the difference was not statistically significant (F = 1.688, P = 0.421). different detection reagents using biuret reagent to detect proteins bias, homogeneity of variance (P = 0.574), a statistically significant difference (F = 5.784, P = 0.011). different calibrators use biuret reagent to detect proteins bias, homogeneity of variance (P = 0.467), the difference was statistically significant (F = 5.289, P = 0.000). CONCLUSION: Biuret reagent in the detection of protein applications, impact detection reagents and calibrators will test result, during the test than when it is necessary to detect deviation detection reagents and calibrators due to be considered.
[Mh] Termos MeSH primário: Biureto/química
Proteínas Sanguíneas/análise
Indicadores e Reagentes/química
[Mh] Termos MeSH secundário: Calibragem
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Proteins); 0 (Indicators and Reagents); 89LJ369D1H (Biuret)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:150518
[Lr] Data última revisão:
150518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150519
[St] Status:MEDLINE


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[PMID]:25051615
[Au] Autor:Hojjatie MM; Abrams D
[Ti] Título:Validation for the determination of biuret in water-soluble, urea-based commercial inorganic fertilizer materials, urea solutions, and sulfur-coated urea products by reversed-phase liquid chromatography: single-laboratory validation of an extension of AOAC official methods 2003.14.
[So] Source:J AOAC Int;97(3):712-20, 2014 May-Jun.
[Is] ISSN:1060-3271
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A single-laboratory validation (SLV) study for the LC determination of biuret in dry and liquid urea-based commercial fertilizers was conducted. A total of 23 samples were used: 11 commercial dry urea products, two urea ammonium nitrate products, eight liquid urea-based commercial fertilizers, and four sulfur-coated urea samples from different sources. In addition, one biuret standard from Aldrich and one sample from the Magruder check sample program were used as validation samples. The proposed method is an extension of AOAC Official Method 2003.14 and is based on dissolution of the test portion in the LC mobile phase and determination by HPLC. The system is linear over a concentration range of 1.00-4.50 mg/L biuret, with a correlation coefficient > or = 0.9999. The biuret was well- separated from urea in the commercial urea samples, and from other constituents in the commercial liquid fertilizer with no observed interferences. Recoveries were determined by spiking four of the validation materials with a known amount of biuret standard and measuring the biuret level according to the method. The averaged recovery was 97%. Method precision was determined by quadruplicate analyses of four of the liquid and six of the commercially available dry urea validation materials using three and four replicate analyses. For the liquid fertilizer analyses, the RSD ranged from 7.04 to 13.31%. For the dry urea analyses, the RSDs ranged from 5.68 to 14.34%. Instrument precision was evaluated at the test initiation by using seven injections of five biuret standard solutions. SD varied from 0.27 to 1.02%, with RSDs averaging 1.14%. The LOD was determined to be 0.009% biuret in material, while the LOQ was determined to be 0.031% biuret in material. In addition to the intralaboratory study, interlaboratory studies were performed by two other outside laboratories using this method. Over a concentration range of 0.2 to 0.9% biuret, the average SD was 0.11%, the average RSD was 21.16%, and the average HorRat value was 4.73%. Furthermore, comparative studies for biuret using AOAC Official Methods 960.04 and 976.01 with the proposed LC method were performed. The three methods produced very close results; however, the two AOAC methods generate hazardous wastes and are more tedious. On the basis of accuracy and precision of the results for this SLV study, it is recommended that this method be collaboratively studied for the determination of biuret in dry and liquid urea-based commercial fertilizer materials.
[Mh] Termos MeSH primário: Biureto/análise
Cromatografia de Fase Reversa/métodos
Fertilizantes/análise
Ureia/análise
[Mh] Termos MeSH secundário: Limite de Detecção
Soluções/química
Enxofre
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Fertilizers); 0 (Solutions); 70FD1KFU70 (Sulfur); 89LJ369D1H (Biuret); 8W8T17847W (Urea)
[Em] Mês de entrada:1408
[Cu] Atualização por classe:140723
[Lr] Data última revisão:
140723
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140724
[St] Status:MEDLINE


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[PMID]:24975994
[Au] Autor:Gupta A; Stockham SL
[Ad] Endereço:Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
[Ti] Título:Negative interference of icteric serum on a bichromatic biuret total protein assay.
[So] Source:Vet Clin Pathol;43(3):422-7, 2014 Sep.
[Is] ISSN:1939-165X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bilirubin is stated to be a negative interferent in some biuret assays and thus could contribute to pseudohypoproteinemia in icteric samples. OBJECTIVE: The purpose of the study was to evaluate the magnitude of and reason for a falsely low total protein concentration in icteric serum when the protein concentration is measured with a bichromatic spectrophotometric biuret assay. METHODS: Commercially available bilirubin was dissolved in 0.1 M NaOH and mixed with sera from 2 dogs to achieve various bilirubin concentrations of up to 40 mg/dL (first set of samples) and 35 mg/dL (second set of samples, for confirmation of first set of results and to explore the interference). Biuret total protein and bilirubin concentrations were determined with a chemistry analyzer (Cobas 6000 with c501 module). Line graphs were drawn to illustrate the effects of increasing bilirubin concentrations on the total protein concentrations. Specific spectrophotometric absorbance readings were examined to identify the reason for the negative interference. RESULTS: High bilirubin concentrations created a negative interference in the Cobas biuret assay. The detectable interference occurred with a spiked bilirubin concentration of 10.7 mg/dL in one set of samples, 20.8 mg/dL in a second set. The interference was due to a greater secondary-absorbance reading at the second measuring point in the samples spiked with bilirubin, which possibly had converted to biliverdin. CONCLUSION: Marked hyperbilirubinemia is associated with a falsely low serum total protein concentration when measured with a bichromatic spectrophotometric biuret assay. This can result in pseudohypoproteinemia and pseudohypoglobulinemia in icteric serum.
[Mh] Termos MeSH primário: Bilirrubina/análise
Biureto/análise
Proteínas Sanguíneas/análise
Doenças do Cão/sangue
Hipoproteinemia/veterinária
[Mh] Termos MeSH secundário: Animais
Cães
Hipoproteinemia/sangue
Refratometria/veterinária
Espectrofotometria/veterinária
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Proteins); 89LJ369D1H (Biuret); RFM9X3LJ49 (Bilirubin)
[Em] Mês de entrada:1506
[Cu] Atualização por classe:161020
[Lr] Data última revisão:
161020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140701
[St] Status:MEDLINE
[do] DOI:10.1111/vcp.12154


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[PMID]:23872452
[Au] Autor:Khademvatan S; Adibpour N; Eskandari A; Rezaee S; Hashemitabar M; Rahim F
[Ad] Endereço:Department of Medical Parasitology, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
[Ti] Título:In silico and in vitro comparative activity of novel experimental derivatives against Leishmania major and Leishmania infantum promastigotes.
[So] Source:Exp Parasitol;135(2):208-16, 2013 Oct.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This in silico and in vitro comparative study was designed to evaluate the effectiveness of some biurets (K1 to K8) and glucantime against Leishmania major and Leishmania infantum promastigotes. Overall, eight experimental ligands and glucantime were docked using AutoDock 4.3 program into the active sites of Leishmania major and Leishmania infantum pteridine reductase 1, which were modeled using homology modeling programs. The colorimetric MTT assay was used to find L. major and L. infantum promastigotes viability at different concentrations of biuret derivatives in a concentration and time-dependent manner and the obtained results were expressed as 50% and 90% of inhibitory concentration (IC50 and IC90). In silico method showed that out of eight experimental ligands, four compounds were more active on pteridine reductase 1. K3 was the most active against L. major promastigotes with an IC50 of 6.8 µM and an IC90 of 40.2 µM, whereas for L. infantum promastigotes was K8 with IC50 of 7.8 µM. The phenylethyl derivative (K7) showed less toxicity (IC50s>60 µM) in both Leishmania strains. Glucantime displayed less growth inhibition in concentration of about 20 µM. In silico and especially docking results in a recent study were in accordance with the in vitro activity of these compounds in presented study and compound K3, K2 and K8 showed reasonable levels of selectivity for the Leishmania pteridine reductase 1.
[Mh] Termos MeSH primário: Antiprotozoários/farmacologia
Biureto/análogos & derivados
Biureto/farmacologia
Leishmania infantum/efeitos dos fármacos
Leishmania major/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antiprotozoários/química
Biureto/química
Colorimetria
Leishmania infantum/citologia
Leishmania major/citologia
Meglumina/farmacologia
Compostos Organometálicos/farmacologia
Filogenia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiprotozoal Agents); 0 (Organometallic Compounds); 6HG8UB2MUY (Meglumine); 75G4TW236W (meglumine antimoniate); 89LJ369D1H (Biuret)
[Em] Mês de entrada:1312
[Cu] Atualização por classe:131014
[Lr] Data última revisão:
131014
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130723
[St] Status:MEDLINE


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[PMID]:23846141
[Au] Autor:Kos I; Jadrijevic-Mladar M; Butula I; Birus M; Maravic-Vlahovicek G; Dabelic S
[Ad] Endereço:Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.
[Ti] Título:Synthesis, antibacterial and cytotoxic activity evaluation of hydroxyurea derivatives.
[So] Source:Acta Pharm;63(2):175-91, 2013 Jun.
[Is] ISSN:1846-9558
[Cp] País de publicação:Croatia
[La] Idioma:eng
[Ab] Resumo:5 Synthesis and biological evaluation of a series (N = 16) of cyclic and acyclic hydroxyurea derivatives, including benzotriazole-, isocyanuric acid- and biuret-containing compounds, are disclosed. 1-N-(benzyloxycarbamoyl)benzotriazole was used as a benzyloxyisocyanate donor, a useful intermediate in the preparation of substituted hydroxyurea. Antibacterial activities of synthesized hydroxyurea derivatives were tested on three E. coli strains, i.e., a strain susceptible to antibiotics, a strain resistant to macrolide antibiotics and a strain resistant to aminoglycoside antibiotics. Six compounds (three acyclic and three cyclic hydroxyureas) showed growth inhibition of the tested E. coli strains, with different specificity toward each strain. Results of the cytotoxic activity evaluation revealed that twelve out of sixteen test compounds were cytotoxic to human acute monocytic leukemia THP-1 and/or human acute T cell leukemia Jurkat cell line. 1-(N-hydroxycarbamoyl) benzotriazole () increased the metabolic activity of both cell lines. Two compounds, 1-(N-hydroxycarbamoyl) benzotriazole (5) and N,N',N''-trihydroxybiuret (15), were identified as potential NO donors.
[Mh] Termos MeSH primário: Biureto
Escherichia coli/efeitos dos fármacos
Hidroxiureia/análogos & derivados
Triazinas
Triazóis
[Mh] Termos MeSH secundário: Antibióticos Antineoplásicos/química
Antibióticos Antineoplásicos/farmacologia
Antineoplásicos/química
Antineoplásicos/farmacologia
Biureto/síntese química
Biureto/farmacologia
Escherichia coli/classificação
Seres Humanos
Isomerismo
Células Jurkat/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Relação Estrutura-Atividade
Triazinas/síntese química
Triazinas/química
Triazinas/farmacologia
Triazóis/síntese química
Triazóis/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibiotics, Antineoplastic); 0 (Antineoplastic Agents); 0 (Triazines); 0 (Triazoles); 86110UXM5Y (benzotriazole); 89LJ369D1H (Biuret); H497R4QKTZ (cyanuric acid); X6Q56QN5QC (Hydroxyurea)
[Em] Mês de entrada:1312
[Cu] Atualização por classe:130712
[Lr] Data última revisão:
130712
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130713
[St] Status:MEDLINE


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[PMID]:23392230
[Au] Autor:Panda C; Dhar BB; Malvi B; Bhattacharjee Y; Gupta SS
[Ad] Endereço:Chemical Engineering Div., National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, India.
[Ti] Título:Catalytic signal amplification using [Fe(III)(biuret-amide)]-mesoporous silica nanoparticles: visual cyanide detection.
[So] Source:Chem Commun (Camb);49(22):2216-8, 2013 Mar 18.
[Is] ISSN:1364-548X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Catalytic signal amplification was used for the colorimetric detection of CN(-) in aqueous media by using the enzyme catalase in tandem with mesoporous silica nanoparticle based synthetic HRP enzyme mimic Fe-MSNs. Signal amplification up to a maximum of eight fold was observed for the reporter "oxidized TMB" with respect to the added CN(-) ion.
[Mh] Termos MeSH primário: Amidas/química
Biureto/química
Catalase/química
Cianetos/análise
Compostos Férricos/química
Nanopartículas/química
Dióxido de Silício/química
[Mh] Termos MeSH secundário: Catalase/metabolismo
Catálise
Colorimetria
Estrutura Molecular
Porosidade
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Amides); 0 (Cyanides); 0 (Ferric Compounds); 7631-86-9 (Silicon Dioxide); 89LJ369D1H (Biuret); EC 1.11.1.6 (Catalase)
[Em] Mês de entrada:1308
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130209
[St] Status:MEDLINE
[do] DOI:10.1039/c3cc38932d


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[PMID]:22730121
[Au] Autor:Seffernick JL; Erickson JS; Cameron SM; Cho S; Dodge AG; Richman JE; Sadowsky MJ; Wackett LP
[Ad] Endereço:Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, St. Paul, Minnesota, USA.
[Ti] Título:Defining sequence space and reaction products within the cyanuric acid hydrolase (AtzD)/barbiturase protein family.
[So] Source:J Bacteriol;194(17):4579-88, 2012 Sep.
[Is] ISSN:1098-5530
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cyanuric acid hydrolases (AtzD) and barbiturases are homologous, found almost exclusively in bacteria, and comprise a rare protein family with no discernible linkage to other protein families or an X-ray structural class. There has been confusion in the literature and in genome projects regarding the reaction products, the assignment of individual sequences as either cyanuric acid hydrolases or barbiturases, and spurious connection of this family to another protein family. The present study has addressed those issues. First, the published enzyme reaction products of cyanuric acid hydrolase are incorrectly identified as biuret and carbon dioxide. The current study employed (13)C nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry to show that cyanuric acid hydrolase releases carboxybiuret, which spontaneously decarboxylates to biuret. This is significant because it revealed that homologous cyanuric acid hydrolases and barbiturases catalyze completely analogous reactions. Second, enzymes that had been annotated incorrectly in genome projects have been reassigned here by bioinformatics, gene cloning, and protein characterization studies. Third, the AtzD/barbiturase family has previously been suggested to consist of members of the amidohydrolase superfamily, a large class of metallohydrolases. Bioinformatics and the lack of bound metals both argue against a connection to the amidohydrolase superfamily. Lastly, steady-state kinetic measurements and observations of protein stability suggested that the AtzD/barbiturase family might be an undistinguished protein family that has undergone some resurgence with the recent introduction of industrial s-triazine compounds such as atrazine and melamine into the environment.
[Mh] Termos MeSH primário: Amidoidrolases/química
Amidoidrolases/metabolismo
Bactérias/enzimologia
Triazinas/metabolismo
[Mh] Termos MeSH secundário: Amidoidrolases/genética
Sequência de Aminoácidos
Azorhizobium caulinodans/enzimologia
Proteínas de Bactérias/química
Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Biureto/metabolismo
Bradyrhizobium/enzimologia
Dados de Sequência Molecular
Moorella/enzimologia
Filogenia
Rhizobium leguminosarum/enzimologia
Alinhamento de Sequência
Análise de Sequência de Proteína
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Triazines); 89LJ369D1H (Biuret); EC 3.5.- (Amidohydrolases); EC 3.5.2.1 (barbiturase); EC 3.5.4.- (cyanuric acid amidohydrolase); H497R4QKTZ (cyanuric acid)
[Em] Mês de entrada:1210
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120626
[St] Status:MEDLINE
[do] DOI:10.1128/JB.00791-12



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