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[PMID]:29363343
[Au] Autor:White C; Wright A; Brightling C
[Ad] Endereço:a Institute for Lung Health, NIHR Leicester Biomedical Research Centre, Department of Infection, Immunity & Inflammation , University of Leicester and University Hospitals of Leicester NHS Trust , Leicester , UK.
[Ti] Título:Fevipiprant in the treatment of asthma.
[So] Source:Expert Opin Investig Drugs;27(2):199-207, 2018 Feb.
[Is] ISSN:1744-7658
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Asthma is common and in many, particularly those with more severe disease, there remains a substantial unmet need. Success with biologics targeting eosinophilic inflammation underscore the value of treating inflammation in asthma beyond corticosteroids. Fevipiprant (QAW039) is an oral treatment for asthma. It competitively and reversibly antagonises the prostaglandin D2 receptor 2 (DP2) expressed on inflammatory and structural cells. Areas covered: We reviewed fevipiprant's mode of action and efficacy against other current and emerging pharmacological interventions for moderate-to-severe asthma. We undertook a literature review using the PubMed/Medline database, the U.S. National Library of Medicine's Clinical Trials website and from manufacturers' press releases with the search terms: 'QAW039', 'Fevipiprant', 'CRTH2 antagonists', 'DP2', 'DP1', 'monoclonal antibody', 'eosinophil' with 'asthma' plus the names of individual drugs. Three Phase 2 trials have been conducted and three Phase 3 trials (NCT02563067, NCT03052517, NCT02555683) are in progress. To date Fevipiprant's greatest success has been in targeting severe eosinophilic asthma. Expert opinion: Fevipiprant presents the possibility of a new orally active therapy for asthma. If successful in phase 3 trials it will have an enormous impact on the treatment paradigm for asthma and will potentially widen access for pre-biologic treatment to a larger population.
[Mh] Termos MeSH primário: Antiasmáticos/uso terapêutico
Asma/tratamento farmacológico
Ácidos Indolacéticos/uso terapêutico
Piridinas/uso terapêutico
[Mh] Termos MeSH secundário: Administração Oral
Antiasmáticos/administração & dosagem
Antiasmáticos/farmacologia
Asma/fisiopatologia
Eosinófilos/metabolismo
Seres Humanos
Ácidos Indolacéticos/administração & dosagem
Ácidos Indolacéticos/farmacologia
Inflamação/tratamento farmacológico
Inflamação/fisiopatologia
Piridinas/administração & dosagem
Piridinas/farmacologia
Índice de Gravidade de Doença
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Asthmatic Agents); 0 (Indoleacetic Acids); 0 (Pyridines); 0 (fevipiprant)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE
[do] DOI:10.1080/13543784.2018.1432592


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[PMID]:28471373
[Au] Autor:Chen Z; Yuan Y; Fu D; Shen C; Yang Y
[Ad] Endereço:College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China. zhchen@hznu.edu.cn.
[Ti] Título:Identification and Expression Profiling of the Auxin Response Factors in Dendrobium officinale under Abiotic Stresses.
[So] Source:Int J Mol Sci;18(5), 2017 May 04.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Auxin response factor (ARF) proteins play roles in plant responses to diverse environmental stresses by binding specifically to the auxin response element in the promoters of target genes. Using our latest public transcriptomes, a comprehensive characterization and analysis of 14 genes were performed. Three selected , including , , and , were confirmed to be nuclear proteins according to their transient expression in epidermal cells of leaves. Furthermore, the transcription activation abilities of , , and were tested in a yeast system. Our data showed that is a transcriptional activator in . To uncover the basic information of gene responses to abiotic stresses, we analyzed their expression patterns under various hormones and abiotic treatments. Based on our data, several hormones and significant stress responsive genes have been identified. Since auxin and genes have been identified in many plant species, our data is imperative to reveal the function of mediated auxin signaling in the adaptation to the challenging environment.
[Mh] Termos MeSH primário: Dendrobium/genética
Regulação da Expressão Gênica de Plantas
Ácidos Indolacéticos/metabolismo
Proteínas de Plantas/genética
Estresse Fisiológico
Fatores de Transcrição/genética
Ativação Transcricional
[Mh] Termos MeSH secundário: Dendrobium/metabolismo
Proteínas de Plantas/metabolismo
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoleacetic Acids); 0 (Plant Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE


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[PMID]:29324818
[Au] Autor:Fujii N; Miyabayashi S; Sugita T; Kobayashi A; Yamazaki C; Miyazawa Y; Kamada M; Kasahara H; Osada I; Shimazu T; Fusejima Y; Higashibata A; Yamazaki T; Ishioka N; Takahashi H
[Ad] Endereço:Graduate School of Life Sciences, Tohoku University, Katahira, Aoba-ku, Sendai, Japan.
[Ti] Título:Root-tip-mediated inhibition of hydrotropism is accompanied with the suppression of asymmetric expression of auxin-inducible genes in response to moisture gradients in cucumber roots.
[So] Source:PLoS One;13(1):e0189827, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In cucumber seedlings, gravitropism interferes with hydrotropism, which results in the nearly complete inhibition of hydrotropism under stationary conditions. However, hydrotropic responses are induced when the gravitropic response in the root is nullified by clinorotation. Columella cells in the root cap sense gravity, which induces the gravitropic response. In this study, we found that removing the root tip induced hydrotropism in cucumber roots under stationary conditions. The application of auxin transport inhibitors to cucumber seedlings under stationary conditions suppressed the hydrotropic response induced by the removal of the root tip. To investigate the expression of genes related to hydrotropism in de-tipped cucumber roots, we conducted transcriptome analysis of gene expression by RNA-Seq using seedlings exhibiting hydrotropic and gravitropic responses. Of the 21 and 45 genes asymmetrically expressed during hydrotropic and gravitropic responses, respectively, five genes were identical. Gene ontology (GO) analysis indicated that the category auxin-inducible genes was significantly enriched among genes that were more highly expressed in the concave side of the root than the convex side during hydrotropic or gravitropic responses. Reverse transcription followed by quantitative polymerase chain reaction (RT-qPCR) analysis revealed that root hydrotropism induced under stationary conditions (by removing the root tip) was accompanied by the asymmetric expression of several auxin-inducible genes. However, intact roots did not exhibit the asymmetric expression patterns of auxin-inducible genes under stationary conditions, even in the presence of a moisture gradient. These results suggest that the root tip inhibits hydrotropism by suppressing the induction of asymmetric auxin distribution. Auxin transport and distribution not mediated by the root tip might play a role in hydrotropism in cucumber roots.
[Mh] Termos MeSH primário: Cucumis sativus/genética
Regulação da Expressão Gênica de Plantas/fisiologia
Gravitropismo/fisiologia
Ácidos Indolacéticos/metabolismo
Raízes de Plantas/fisiologia
[Mh] Termos MeSH secundário: Cucumis sativus/crescimento & desenvolvimento
Genes de Plantas
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Transcriptoma
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Indoleacetic Acids); 059QF0KO0R (Water)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189827


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[PMID]:28465425
[Au] Autor:Brown KM; Long S; Sibley LD
[Ad] Endereço:Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri, USA.
[Ti] Título:Plasma Membrane Association by N-Acylation Governs PKG Function in .
[So] Source:MBio;8(3), 2017 May 02.
[Is] ISSN:2150-7511
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cyclic GMP (cGMP)-dependent protein kinase (protein kinase G [PKG]) is essential for microneme secretion, motility, invasion, and egress in apicomplexan parasites, However, the separate roles of two isoforms of the kinase that are expressed by some apicomplexans remain uncertain. Despite having identical regulatory and catalytic domains, PKG is plasma membrane associated whereas PKG is cytosolic in To determine whether these isoforms are functionally distinct or redundant, we developed an auxin-inducible degron (AID) tagging system for conditional protein depletion in By combining AID regulation with genome editing strategies, we determined that PKG is necessary and fully sufficient for PKG-dependent cellular processes. Conversely, PKG is functionally insufficient and dispensable in the presence of PKG The difference in functionality mapped to the first 15 residues of PKG , containing a myristoylated Gly residue at position 2 that is critical for membrane association and PKG function. Collectively, we have identified a novel requirement for cGMP signaling at the plasma membrane and developed a new system for examining essential proteins in is an obligate intracellular apicomplexan parasite and important clinical and veterinary pathogen that causes toxoplasmosis. Since apicomplexans can only propagate within host cells, efficient invasion is critically important for their life cycles. Previous studies using chemical genetics demonstrated that cyclic GMP signaling through protein kinase G (PKG)-controlled invasion by apicomplexan parasites. However, these studies did not resolve functional differences between two compartmentalized isoforms of the kinase. Here we developed a conditional protein regulation tool to interrogate PKG isoforms in We found that the cytosolic PKG isoform was largely insufficient and dispensable. In contrast, the plasma membrane-associated isoform was necessary and fully sufficient for PKG function. Our studies identify the plasma membrane as a key location for PKG activity and provide a broadly applicable system for examining essential proteins in .
[Mh] Termos MeSH primário: Membrana Celular/metabolismo
Proteínas Quinases Dependentes de GMP Cíclico/metabolismo
Proteínas de Protozoários/metabolismo
Toxoplasma/metabolismo
[Mh] Termos MeSH secundário: Acilação
Animais
GMP Cíclico/metabolismo
Proteínas Quinases Dependentes de GMP Cíclico/química
Proteínas Quinases Dependentes de GMP Cíclico/classificação
Edição de Genes
Seres Humanos
Ácidos Indolacéticos
Fosforilação
Isoformas de Proteínas/metabolismo
Proteínas de Protozoários/química
Transdução de Sinais
Toxoplasma/citologia
Toxoplasma/genética
Toxoplasmose/metabolismo
Toxoplasmose/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoleacetic Acids); 0 (Protein Isoforms); 0 (Protozoan Proteins); EC 2.7.11.12 (Cyclic GMP-Dependent Protein Kinases); H2D2X058MU (Cyclic GMP)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180126
[Lr] Data última revisão:
180126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


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[PMID]:27773568
[Au] Autor:Rakusová H; Abbas M; Han H; Song S; Robert HS; Friml J
[Ad] Endereço:Institute of Science and Technology (IST) Austria, 3400 Klosterneuburg, Austria; Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.
[Ti] Título:Termination of Shoot Gravitropic Responses by Auxin Feedback on PIN3 Polarity.
[So] Source:Curr Biol;26(22):3026-3032, 2016 Nov 21.
[Is] ISSN:1879-0445
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plants adjust their growth according to gravity. Gravitropism involves gravity perception, signal transduction, and asymmetric growth response, with organ bending as a consequence [1]. Asymmetric growth results from the asymmetric distribution of the plant-specific signaling molecule auxin [2] that is generated by lateral transport, mediated in the hypocotyl predominantly by the auxin transporter PIN-FORMED3 (PIN3) [3-5]. Gravity stimulation polarizes PIN3 to the bottom sides of endodermal cells, correlating with increased auxin accumulation in adjacent tissues at the lower side of the stimulated organ, where auxin induces cell elongation and, hence, organ bending. A curvature response allows the hypocotyl to resume straight growth at a defined angle [6], implying that at some point auxin symmetry is restored to prevent overbending. Here, we present initial insights into cellular and molecular mechanisms that lead to the termination of the tropic response. We identified an auxin feedback on PIN3 polarization as underlying mechanism that restores symmetry of the PIN3-dependent auxin flow. Thus, two mechanistically distinct PIN3 polarization events redirect auxin fluxes at different time points of the gravity response: first, gravity-mediated redirection of PIN3-mediated auxin flow toward the lower hypocotyl side, where auxin gradually accumulates and promotes growth, and later PIN3 polarization to the opposite cell side, depleting this auxin maximum to end the bending. Accordingly, genetic or pharmacological interference with the late PIN3 polarization prevents termination of the response and leads to hypocotyl overbending. This observation reveals a role of auxin feedback on PIN polarity in the termination of the tropic response.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Arabidopsis/fisiologia
Gravitropismo
Sensação Gravitacional
Ácidos Indolacéticos/metabolismo
Reguladores de Crescimento de Planta/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/crescimento & desenvolvimento
Proteínas de Arabidopsis/metabolismo
Retroalimentação Fisiológica
Brotos de Planta/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Indoleacetic Acids); 0 (PIN3 protein, Arabidopsis); 0 (Plant Growth Regulators)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:29200506
[Au] Autor:Keller CP
[Ad] Endereço:Department of Biology, Minot State University, 500 University Avenue West, Minot, North Dakota 58707.
[Ti] Título:Leaf expansion in : transient auxin-induced growth increase.
[So] Source:Physiol Plant;130(4):580-589, 2017 Aug.
[Is] ISSN:1399-3054
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Control of leaf expansion by auxin is not well understood. Evidence from short term exogenous applications and from treatment of excised tissues suggests auxin positively influences growth. Manipulations of endogenous leaf auxin content, however, suggests that, long-term, auxin suppresses leaf expansion. This study attempts to clarify the growth effects of auxin on unifoliate (primary) leaves of the common bean ( ) by reexamining the response to auxin treatment of both excised leaf strips and attached leaves. Leaf strips, incubated in culture conditions that promoted steady elongation for up to 48 h, treated with 10 µM NAA responded with an initial surge of elongation growth complete within 10 hours followed by insensitivity. A range of NAA concentrations from 0.1 µM to 300 µM induced increased strip elongation after 24 hours and 48 hours. Increased elongation and epinastic curvature of leaf strips was found specific to active auxins. Expanding attached unifoliates treated once with aqueous auxin α-naphthalene acetic acid (NAA) at 1.0 mM showed both an initial surge in growth lasting 4-6 hours followed by growth inhibition sustained at least as long as 24 hours post treatment. Auxin-induced inhibition of leaf expansion was associated with smaller epidermal cell area. Together the results suggest increasing leaf auxin first increases growth then slows growth through inhibition of cell expansion. Excised leaf strips, retain only the initial increased growth response to auxin and not the subsequent growth inhibition, either as a consequence of wounding or of isolation from the plant.
[Mh] Termos MeSH primário: Ácidos Indolacéticos/farmacologia
Phaseolus/crescimento & desenvolvimento
Reguladores de Crescimento de Planta/farmacologia
Folhas de Planta/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Ácidos Naftalenoacéticos/farmacologia
Phaseolus/efeitos dos fármacos
Folhas de Planta/efeitos dos fármacos
Técnicas de Cultura de Tecidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indoleacetic Acids); 0 (Naphthaleneacetic Acids); 0 (Plant Growth Regulators); 33T7G7757C (1-naphthaleneacetic acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE
[do] DOI:10.1111/j.1399-3054.2007.00916.x


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[PMID]:29220348
[Au] Autor:Ligerot Y; de Saint Germain A; Waldie T; Troadec C; Citerne S; Kadakia N; Pillot JP; Prigge M; Aubert G; Bendahmane A; Leyser O; Estelle M; Debellé F; Rameau C
[Ad] Endereço:Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, Versailles, France.
[Ti] Título:The pea branching RMS2 gene encodes the PsAFB4/5 auxin receptor and is involved in an auxin-strigolactone regulation loop.
[So] Source:PLoS Genet;13(12):e1007089, 2017 Dec.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Strigolactones (SLs) are well known for their role in repressing shoot branching. In pea, increased transcript levels of SL biosynthesis genes are observed in stems of highly branched SL deficient (ramosus1 (rms1) and rms5) and SL response (rms3 and rms4) mutants indicative of negative feedback control. In contrast, the highly branched rms2 mutant has reduced transcript levels of SL biosynthesis genes. Grafting studies and hormone quantification led to a model where RMS2 mediates a shoot-to-root feedback signal that regulates both SL biosynthesis gene transcript levels and xylem sap levels of cytokinin exported from roots. Here we cloned RMS2 using synteny with Medicago truncatula and demonstrated that it encodes a putative auxin receptor of the AFB4/5 clade. Phenotypes similar to rms2 were found in Arabidopsis afb4/5 mutants, including increased shoot branching, low expression of SL biosynthesis genes and high auxin levels in stems. Moreover, afb4/5 and rms2 display a specific resistance to the herbicide picloram. Yeast-two-hybrid experiments supported the hypothesis that the RMS2 protein functions as an auxin receptor. SL root feeding using hydroponics repressed auxin levels in stems and down-regulated transcript levels of auxin biosynthesis genes within one hour. This auxin down-regulation was also observed in plants treated with the polar auxin transport inhibitor NPA. Together these data suggest a homeostatic feedback loop in which auxin up-regulates SL synthesis in an RMS2-dependent manner and SL down-regulates auxin synthesis in an RMS3 and RMS4-dependent manner.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/genética
Ervilhas/genética
Proteínas de Plantas/genética
Receptores de Superfície Celular/genética
[Mh] Termos MeSH secundário: Arabidopsis/genética
Proteínas de Arabidopsis/metabolismo
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/genética
Ácidos Indolacéticos/metabolismo
Medicago truncatula/genética
Ervilhas/crescimento & desenvolvimento
Picloram/farmacologia
Reguladores de Crescimento de Planta/genética
Reguladores de Crescimento de Planta/metabolismo
Proteínas de Plantas/metabolismo
Brotos de Planta/efeitos dos fármacos
Brotos de Planta/genética
Brotos de Planta/crescimento & desenvolvimento
Receptores de Superfície Celular/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (AFB4 protein, Arabidopsis); 0 (AFB5 protein, Arabidopsis); 0 (Arabidopsis Proteins); 0 (Indoleacetic Acids); 0 (Plant Growth Regulators); 0 (Plant Proteins); 0 (Receptors, Cell Surface); 0 (auxin receptor, plant); O7437X49DW (Picloram)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171231
[Lr] Data última revisão:
171231
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1007089


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[PMID]:28456120
[Au] Autor:Denisov Y; Glick S; Zviran T; Ish-Shalom M; Levin A; Faigenboim A; Cohen Y; Irihimovitch V
[Ad] Endereço:Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, Bet-Dagan 50250, Israel.
[Ti] Título:Distinct organ-specific and temporal expression profiles of auxin-related genes during mango fruitlet drop.
[So] Source:Plant Physiol Biochem;115:439-448, 2017 Jun.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:In mango, fruitlet abscission initiates with a decrease in polar auxin transport through the abscission zone (AZ), triggered by ethylene. To explore the molecular components affecting this process, we initially conducted experiments with developing fruitlet explants in which fruitlet drop was induced by ethephon, and monitored the expression patterns of distinct indole-3-acetic acid (IAA)-related genes, comparing control vs. ethephon-treated pericarp and AZ profiles. Over the examined time period (48 h), the accumulation of MiPIN1 and MiLAX2 IAA-efflux and influx genes decreased in both control and treated tissues. Nevertheless, ethephon-treated tissues displayed significantly lower levels of these transcripts within 18-24 h. An opposite pattern was observed for MiLAX3, which overall exhibited up-regulation in treated fruitlet tissues. Ethephon treatment also induced an early and pronounced down-regulation of five out of six IAA-responsive genes, and a substantial reduction in the accumulation of two IAA-synthesis related transcripts, contrasting with significant up-regulation of Gretchen Hagen3 transcript (MiGH3.1) encoding an IAA-amino synthetase. Furthermore, for both control and treated AZ, the decrease in IAA-carrier transcripts was associated with a decrease in IAA content and an increase in IAA-Asp:IAA ratio, suggesting that fruitlet drop is accompanied by formation of this non-hydrolyzed IAA-amino acid conjugate. Despite these similarities, ethephon-treated AZ displayed a sharper decrease in IAA content and higher IAA-Asp:IAA ratio within 18 h. Lastly, the response of IAA-related genes to exogenous IAA treatment was also examined. Our results are discussed, highlighting the roles that distinct IAA-related genes might assume during mango fruitlet drop.
[Mh] Termos MeSH primário: Mangifera/metabolismo
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Etilenos/metabolismo
Regulação da Expressão Gênica de Plantas/genética
Regulação da Expressão Gênica de Plantas/fisiologia
Ácidos Indolacéticos/metabolismo
Mangifera/genética
Proteínas de Plantas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenes); 0 (Indoleacetic Acids); 0 (Plant Proteins); 91GW059KN7 (ethylene)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:28448840
[Au] Autor:Bhattacharyya D; Lee YH
[Ad] Endereço:Division of Biotechnology, Chonbuk National University, 79 Gobong-ro, Iksan-si, Jeollabuk-do 54596, Republic of Korea.
[Ti] Título:A cocktail of volatile compounds emitted from Alcaligenes faecalis JBCS1294 induces salt tolerance in Arabidopsis thaliana by modulating hormonal pathways and ion transporters.
[So] Source:J Plant Physiol;214:64-73, 2017 Jul.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In our previous study we showed that volatile organic compounds (VOCs) from Alcaligenes faecalis JBCS1294 (JBCS1294) induced tolerance to salt stress in Arabidopsis thaliana by influencing the auxin and gibberellin pathways and upregulating the expression of key ion transporters. The aim of this study was to evaluate the contribution of each VOC and blends of the VOCs on the induction of salt tolerance and signaling pathways. The key VOCs emitted from JBCS1294 were dissolved in lanolin and applied to one side of bipartite I-plates that contained Arabidopsis seeds on Murashige and Skoog (MS) media supplemented with NaCl on the other side. Changes in plant growth were investigated using Arabidopsis mutant lines and hormone inhibitors, and gene expression was assessed by real-time PCR (qPCR). Among the VOCs, butyric acid conferred salt tolerance over a concentration range of 5.6µM (10ng)-56mM (100µg), whereas propionic and benzoic acid were effective at micromolar doses. Intriguingly, the optimized cocktail of the three VOCs increased fresh weight of Arabidopsis under salt stress compared to that achieved with each single compound. However, Arabidopsis growth was not promoted by the VOCs without salt stress. Exogenous indole-3-acetic acid (IAA) application arrested salt tolerance or growth promotion of Arabidopsis induced by volatiles from propionic acid, but not from butyric acid and an optimized volatile mixture of butyric acid, propionic acid, and benzoic acid (1PBB). High and intense auxin-responsive DR5:GUS activity was observed in the roots of Arabidopsis grown on media without salt via 1PBB, butyric acid, and benzoic acid. Growth promotion by the cocktail was inhibited in the eir1 mutant and in Col-0 plants treated with inhibitors of auxin and gibberellin. The present study clearly demonstrated the effects of individual VOCs and blends of VOCs from a rhizobacterial strain on the induction of salt stress. The results with the blend of VOCs, which mimics bacterial emissions in nature, may lead to a deeper understanding of the interaction between rhizobacteria and plants.
[Mh] Termos MeSH primário: Alcaligenes faecalis/química
Arabidopsis/efeitos dos fármacos
Arabidopsis/metabolismo
Compostos Orgânicos Voláteis/farmacologia
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Giberelinas/metabolismo
Ácidos Indolacéticos/metabolismo
Tolerância a Sal/efeitos dos fármacos
Compostos Orgânicos Voláteis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gibberellins); 0 (Indoleacetic Acids); 0 (Volatile Organic Compounds)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE


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[PMID]:27770560
[Au] Autor:Zhang Y; Yu Q; Jiang N; Yan X; Wang C; Wang Q; Liu J; Zhu M; Bednarek SY; Xu J; Pan J
[Ad] Endereço:College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004, China.
[Ti] Título:Clathrin regulates blue light-triggered lateral auxin distribution and hypocotyl phototropism in Arabidopsis.
[So] Source:Plant Cell Environ;40(1):165-176, 2017 Jan.
[Is] ISSN:1365-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Phototropism is the process by which plants grow towards light in order to maximize the capture of light for photosynthesis, which is particularly important for germinating seedlings. In Arabidopsis, hypocotyl phototropism is predominantly triggered by blue light (BL), which has a profound effect on the establishment of asymmetric auxin distribution, essential for hypocotyl phototropism. Two auxin efflux transporters ATP-binding cassette B19 (ABCB19) and PIN-formed 3 (PIN3) are known to mediate the effect of BL on auxin distribution in the hypocotyl, but the details for how BL triggers PIN3 lateralization remain poorly understood. Here, we report a critical role for clathrin in BL-triggered, PIN3-mediated asymmetric auxin distribution in hypocotyl phototropism. We show that unilateral BL induces relocalization of clathrin in the hypocotyl. Loss of clathrin light chain 2 (CLC2) and CLC3 affects endocytosis and lateral distribution of PIN3 thereby impairing BL-triggered establishment of asymmetric auxin distribution and consequently, phototropic bending. Conversely, auxin efflux inhibitors N-1-naphthylphthalamic acid and 2,3,5-triiodobenzoic acid affect BL-induced relocalization of clathrin, endocytosis and lateralization of PIN3 as well as asymmetric distribution of auxin. These results together demonstrate an important interplay between auxin and clathrin function that dynamically regulates BL-triggered hypocotyl phototropism in Arabidopsis.
[Mh] Termos MeSH primário: Arabidopsis/fisiologia
Arabidopsis/efeitos da radiação
Clatrina/metabolismo
Hipocótilo/fisiologia
Ácidos Indolacéticos/metabolismo
Luz
Fototropismo/efeitos da radiação
[Mh] Termos MeSH secundário: Proteínas de Arabidopsis/metabolismo
Endocitose/efeitos da radiação
Hipocótilo/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); 0 (Clathrin); 0 (Indoleacetic Acids)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171212
[Lr] Data última revisão:
171212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE
[do] DOI:10.1111/pce.12854



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