Base de dados : MEDLINE
Pesquisa : D03.132.098.038 [Categoria DeCS]
Referências encontradas : 913 [refinar]
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[PMID]:29045420
[Au] Autor:Zhang QS; Kurpad DS; Mahoney MG; Steinbeck MJ; Freeman TA
[Ad] Endereço:Department of Orthopaedic Surgery, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, United States of America.
[Ti] Título:Inhibition of apoptosis signal-regulating kinase 1 alters the wound epidermis and enhances auricular cartilage regeneration.
[So] Source:PLoS One;12(10):e0185803, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Why regeneration does not occur in mammals remains elusive. In lower vertebrates, epimorphic regeneration of the limb is directed by the wound epidermis, which controls blastema formation to promote regrowth of the appendage. Herein, we report that knockout (KO) or inhibition of Apoptosis Signal-regulated Kinase-1 (ASK1), also known as mitogen-activated protein kinase kinase kinase 5 (MAP3K5), after full thickness ear punch in mice prolongs keratinocyte activation within the wound epidermis and promotes regeneration of auricular cartilage. Histological analysis showed the ASK1 KO ears displayed enhanced protein markers associated with blastema formation, hole closure and regeneration of auricular cartilage. At seven days after punch, the wound epidermis morphology was markedly different in the KO, showing a thickened stratum corneum with rounded cell morphology and a reduction of both the granular cell layer and decreased expression of filament aggregating protein. In addition, cytokeratin 6 was expressed in the stratum spinosum and granulosum. Topical application of inhibitors of ASK1 (NQDI-1), the upstream ASK1 activator, calcium activated mitogen kinase 2 (KN93), or the downstream target, c-Jun N-terminal kinase (SP600125) also resulted in enhanced regeneration; whereas inhibition of the other downstream target, the p38 α/ß isoforms, (SB203580) had no effect. The results of this investigation indicate ASK1 inhibition prolongs keratinocyte and blastemal cell activation leading to ear regeneration.
[Mh] Termos MeSH primário: Cartilagem da Orelha/patologia
Epiderme/patologia
MAP Quinase Quinase Quinase 5/antagonistas & inibidores
Regeneração
Ferimentos e Lesões/patologia
[Mh] Termos MeSH secundário: Animais
Aporfinas/farmacologia
Membrana Basal/efeitos dos fármacos
Membrana Basal/metabolismo
Biomarcadores/metabolismo
Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina/metabolismo
Diferenciação Celular/efeitos dos fármacos
Cartilagem da Orelha/efeitos dos fármacos
Epiderme/efeitos dos fármacos
Epitélio/patologia
Isoenzimas/metabolismo
Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo
Queratinócitos/efeitos dos fármacos
Queratinócitos/patologia
MAP Quinase Quinase Quinase 5/metabolismo
Camundongos Endogâmicos C57BL
Camundongos Knockout
Quinolinas/farmacologia
Regeneração/efeitos dos fármacos
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (2,7-dioxo-2,7-dihydro-3H-naphtho(1,2,3-de)quinoline-1-carboxylate); 0 (Aporphines); 0 (Biomarkers); 0 (Isoenzymes); 0 (Quinolines); EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2); EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases); EC 2.7.11.25 (MAP Kinase Kinase Kinase 5); EC 2.7.11.25 (Map3k5 protein, mouse)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171019
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185803


  2 / 913 MEDLINE  
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[PMID]:28821607
[Au] Autor:Tripathi SK; Xu T; Feng Q; Avula B; Shi X; Pan X; Mask MM; Baerson SR; Jacob MR; Ravu RR; Khan SI; Li XC; Khan IA; Clark AM; Agarwal AK
[Ad] Endereço:From the National Center for Natural Products Research.
[Ti] Título:Two plant-derived aporphinoid alkaloids exert their antifungal activity by disrupting mitochondrial iron-sulfur cluster biosynthesis.
[So] Source:J Biol Chem;292(40):16578-16593, 2017 Oct 06.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Eupolauridine and liriodenine are plant-derived aporphinoid alkaloids that exhibit potent inhibitory activity against the opportunistic fungal pathogens and However, the molecular mechanism of this antifungal activity is unknown. In this study, we show that eupolauridine 9591 (E9591), a synthetic analog of eupolauridine, and liriodenine methiodide (LMT), a methiodide salt of liriodenine, mediate their antifungal activities by disrupting mitochondrial iron-sulfur (Fe-S) cluster synthesis. Several lines of evidence supported this conclusion. First, both E9591 and LMT elicited a transcriptional response indicative of iron imbalance, causing the induction of genes that are required for iron uptake and for the maintenance of cellular iron homeostasis. Second, a genome-wide fitness profile analysis showed that yeast mutants with deletions in iron homeostasis-related genes were hypersensitive to E9591 and LMT. Third, treatment of wild-type yeast cells with E9591 or LMT generated cellular defects that mimicked deficiencies in mitochondrial Fe-S cluster synthesis including an increase in mitochondrial iron levels, a decrease in the activities of Fe-S cluster enzymes, a decrease in respiratory function, and an increase in oxidative stress. Collectively, our results demonstrate that E9591 and LMT perturb mitochondrial Fe-S cluster biosynthesis; thus, these two compounds target a cellular pathway that is distinct from the pathways commonly targeted by clinically used antifungal drugs. Therefore, the identification of this pathway as a target for antifungal compounds has potential applications in the development of new antifungal therapies.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Aporfinas/farmacologia
Candida albicans
Proteínas Fúngicas
Indenos/farmacologia
Proteínas com Ferro-Enxofre
Proteínas Mitocondriais
Naftiridinas/farmacologia
[Mh] Termos MeSH secundário: Antifúngicos/química
Aporfinas/química
Candida albicans/genética
Candida albicans/crescimento & desenvolvimento
Cryptococcus neoformans/genética
Cryptococcus neoformans/crescimento & desenvolvimento
Proteínas Fúngicas/genética
Proteínas Fúngicas/metabolismo
Estudo de Associação Genômica Ampla
Indenos/química
Proteínas com Ferro-Enxofre/genética
Proteínas com Ferro-Enxofre/metabolismo
Proteínas Mitocondriais/genética
Proteínas Mitocondriais/metabolismo
Naftiridinas/química
Estresse Oxidativo/efeitos dos fármacos
Estresse Oxidativo/genética
Consumo de Oxigênio/efeitos dos fármacos
Consumo de Oxigênio/genética
Saccharomyces cerevisiae
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Aporphines); 0 (Fungal Proteins); 0 (Indenes); 0 (Iron-Sulfur Proteins); 0 (Mitochondrial Proteins); 0 (Naphthyridines); 58786-39-3 (eupolauridine); E134R7X4O9 (liriodenine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170820
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.781773


  3 / 913 MEDLINE  
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[PMID]:28703314
[Au] Autor:Le PM; Srivastava V; Nguyen TT; Pradines B; Madamet M; Mosnier J; Trinh TT; Lee H
[Ad] Endereço:Measurement Science and Standards, National Research Council Canada, 1200 Montreal Road, Ottawa, ON, K1A 0R6, Canada.
[Ti] Título:Stephanine from Stephania venosa (Blume) Spreng Showed Effective Antiplasmodial and Anticancer Activities, the Latter by Inducing Apoptosis through the Reverse of Mitotic Exit.
[So] Source:Phytother Res;31(9):1357-1368, 2017 Sep.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Extracts from the tubers of Stephania venosa (Blum) Spreng growing in Vietnam significantly inhibited cell proliferation against a number of cancer cells including HeLa, MDA-MB231 and MCF-7 cells. A bioassay-guided fractionation led to the isolation of four aporphine and one tetrahydroprotoberberine alkaloids: dehydrocrebanine 1, tetrahydropalmatine 2, stephanine 3, crebanine 4 and O-methylbulbocapnine 5. The characterization of these compounds was based on MS, NMR and published data. A study by structure-bioactivity relationship on these isolates showed that stephanine is the most active compound. Cell biological studies showed that stephanine induces the reverse of mitotic exit, eventually leading to cell death by apoptosis. This data suggests that stephanine has a unique mode of cell-killing activity against cancer cells, which is seldom observed with known synthetic compounds. In addition to its anticancer property, our data from an in vitro study showed that S. venosa also possesses effective antiplasmodial activity and stephanine was also the most interesting compound but is the most cytotoxic with the lowest selectivity index. Copyright © 2017 Her Majesty the Queen in Right of Canada Phytotherapy Research StartCopTextCopyright © 2017 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Antimaláricos/farmacologia
Antineoplásicos Fitogênicos/farmacologia
Apoptose/efeitos dos fármacos
Aporfinas/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Alcaloides/farmacologia
Alcaloides de Berberina/química
Alcaloides de Berberina/farmacologia
Seres Humanos
Células MCF-7
Mitose/efeitos dos fármacos
Estrutura Molecular
Fitoterapia
Tubérculos/química
Plasmodium falciparum/efeitos dos fármacos
Stephania/química
Vietnã
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Antimalarials); 0 (Antineoplastic Agents, Phytogenic); 0 (Aporphines); 0 (Berberine Alkaloids); 0 (Plant Extracts); 13NS2KTD6H (aporphine); 25127-29-1 (crebanine); 3X69CO5I79 (tetrahydropalmatine); 517-63-5 (stephanine); 728C74FB5Z (berbine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5861


  4 / 913 MEDLINE  
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[PMID]:28414230
[Au] Autor:Bakiri A; Hubert J; Reynaud R; Lanthony S; Harakat D; Renault JH; Nuzillard JM
[Ad] Endereço:Institut de Chimie Moléculaire de Reims, UMR CNRS 7312, SFR CAP'SANTE, Université de Reims Champagne-Ardenne , Reims 51097, France.
[Ti] Título:Computer-Aided C NMR Chemical Profiling of Crude Natural Extracts without Fractionation.
[So] Source:J Nat Prod;80(5):1387-1396, 2017 May 26.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A computer-aided, C NMR-based dereplication method is presented for the chemical profiling of natural extracts without any fractionation. An algorithm was developed in order to compare the C NMR chemical shifts obtained from a single routine spectrum with a set of predicted NMR data stored in a natural metabolite database. The algorithm evaluates the quality of the matching between experimental and predicted data by calculating a score function and returns the list of metabolites that are most likely to be present in the studied extract. The proof of principle of the method is demonstrated on a crude alkaloid extract obtained from the leaves of Peumus boldus, resulting in the identification of eight alkaloids, including isocorydine, rogersine, boldine, reticuline, coclaurine, laurotetanine, N-methylcoclaurine, and norisocorydine, as well as three monoterpenes, namely, p-cymene, eucalyptol, and α-terpinene. The results were compared to those obtained with other methods, either involving a fractionation step before the chemical profiling process or using mass spectrometry detection in the infusion mode or coupled to gas chromatography.
[Mh] Termos MeSH primário: Alcaloides/análise
Aporfinas/química
Espectroscopia de Ressonância Magnética Nuclear de Carbono-13/métodos
Monoterpenos/análise
Monoterpenos/química
Peumus/química
Folhas de Planta/química
[Mh] Termos MeSH secundário: Alcaloides/química
Espectrometria de Massas
Estrutura Molecular
Extratos Vegetais/análise
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Aporphines); 0 (Monoterpenes); 0 (Plant Extracts); 1G1C8T1N7Q (4-cymene); 3B5E87JEOX (isocorydine); 4YGF4PQP49 (gamma-terpinene); SDW3N623LN (laurotetanine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b01063


  5 / 913 MEDLINE  
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[PMID]:28264467
[Au] Autor:Liu X; Tian H; Li H; Ge C; Zhao F; Yao M; Li J
[Ad] Endereço:State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine, 25/Ln 2200, Xietu Road, Shanghai 200032, China. liuxiaoqin106484@163.com.
[Ti] Título:Derivate Isocorydine (d-ICD) Suppresses Migration and Invasion of Hepatocellular Carcinoma Cell by Downregulating ITGA1 Expression.
[So] Source:Int J Mol Sci;18(3), 2017 Feb 27.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:In our previous studies, we found that isocorydine (ICD) could be a potential antitumor agent in hepatocellular carcinoma (HCC). Derivate isocorydine (d-ICD), a more effective antitumor agent, has been demonstrated to inhibit proliferation and drug resistance in HCC. In order to investigate the potential role of d-ICD on HCC cell migration and its possible mechanism, wound healing assay, trans-well invasion assay, western blot analysis, and qRT-PCR were performed to study the migration and invasion ability of HCC cells as well as relevant molecular alteration following d-ICD treatment. Results indicated that the migration and invasion ability of HCC cells were suppressed when cultured with d-ICD. Meanwhile, the expression level of was markedly reduced. Furthermore, we found that promotes HCC cell migration and invasion in vitro, and that can partly reverse the effect of d-ICD-induced migration and invasion suppression in HCC cells. In addition, dual luciferase reporter assay and chromatin immunoprecipitation assay were used to study the expression regulation of , and found that directly upregulates expression and d-ICD inhibits expression. Taken together, these results reveal that d-ICD inhibits HCC cell migration and invasion may partly by downregulating / expression.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Aporfinas/farmacologia
Movimento Celular/efeitos dos fármacos
Expressão Gênica
Integrina alfa1/genética
[Mh] Termos MeSH secundário: Carcinoma Hepatocelular/metabolismo
Linhagem Celular Tumoral
Fator de Transcrição E2F1/metabolismo
Inativação Gênica
Seres Humanos
Neoplasias Hepáticas/metabolismo
MicroRNAs/genética
Regiões Promotoras Genéticas
Ativação Transcricional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Aporphines); 0 (E2F1 Transcription Factor); 0 (Integrin alpha1); 0 (MicroRNAs); 3B5E87JEOX (isocorydine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170420
[Lr] Data última revisão:
170420
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE


  6 / 913 MEDLINE  
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[PMID]:28259252
[Au] Autor:Lin CZ; Liu ZJ; Bairi ZD; Zhu CC
[Ad] Endereço:Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China.
[Ti] Título:A new diterpenoid alkaloid isolated from Delphinium caeruleum.
[So] Source:Chin J Nat Med;15(1):45-48, 2017 Jan.
[Is] ISSN:1875-5364
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:The present study was designed to determine the chemical constituents of Delphinium caeruleum Jacq. ex Camb.. The chemical constituents were isolated and purified by column chromatography with silica gel, ODS, and Sephadex LH-20. Their structures were elucidated by IR, MS, and NMR. Ten compounds were obtained and identified as caerudelphinine A (1), lycoctonine (2), talitine B (3), talitine A (4), talitine C (5), tatsienine-V (6), d-magnoflorine (7), 2-trimethyl-ammonio-3-(3-indolyl) propionate (8), vakhmatine (9), and delatisine (10). Compound 1 was a new lycoctonine-type C19-diterpenoid alkaloid, and compounds 4-10 were isolated from this plant for the first time.
[Mh] Termos MeSH primário: Delphinium/química
Diterpenos/isolamento & purificação
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Aconitina/análogos & derivados
Aconitina/química
Aconitina/isolamento & purificação
Alcaloides/química
Alcaloides/isolamento & purificação
Aporfinas/química
Aporfinas/isolamento & purificação
Diterpenos/química
Espectroscopia de Ressonância Magnética
Estrutura Molecular
Componentes Aéreos da Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Aporphines); 0 (Diterpenes); 0 (Plant Extracts); 0 (caerudelphinine A); 0GLX1UNC80 (lycoctonine); 86695-18-3 (tatsiensine); NI8K6962K4 (magnoflorine); X8YN71D5WC (Aconitine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170306
[St] Status:MEDLINE


  7 / 913 MEDLINE  
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[PMID]:28165858
[Au] Autor:Liu Y; Wu X; Mi Y; Zhang B; Gu S; Liu G; Li X
[Ad] Endereço:a Department of Clinical Pharmacy.
[Ti] Título:PLGA nanoparticles for the oral delivery of nuciferine: preparation, physicochemical characterization and in vitro/in vivo studies.
[So] Source:Drug Deliv;24(1):443-451, 2017 Nov.
[Is] ISSN:1521-0464
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This article reports a promising approach to enhance the oral delivery of nuciferine (NUC), improve its aqueous solubility and bioavailability, and allow its controlled release as well as inhibiting lipid accumulation. NUC-loaded poly lactic-co-glycolic acid nanoparticles (NUC-PLGA-NPs) were prepared according to a solid/oil/water (s/o/w) emulsion technique due to the water-insolubility of NUC. PLGA exhibited excellent loading capacity for NUC with adjustable dosing ratios. The drug loading and encapsulation efficiency of optimized formulation were 8.89 ± 0.71 and 88.54 ± 7.08%, respectively. NUC-PLGA-NPs exhibited a spherical morphology with average size of 150.83 ± 5.72 nm and negative charge of -22.73 ± 1.63 mV, which are suitable for oral administration. A sustained NUC released from NUC-PLGA-NPs with an initial exponential release owing to the surface associated drug followed by a slower release of NUC, which was entrapped in the core. In addition, ∼77 ± 6.67% was released in simulating intestinal juice, while only about 45.95 ± 5.2% in simulating gastric juice. NUC-PLGA-NPs are more efficient against oleic acid (OA)-induced hepatic steatosis in HepG cells when compared to naked NUC (n-NUC, *p < 0.05). The oral bioavailability of NUC-PLGA-NPs group was significantly higher (**p < 0.01) and a significantly decreased serum levels of total cholesterol (TC), triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C), as well as a higher concentration of high-density lipoprotein cholesterol (HDL-C) was observed, compared with that of n-NUC treated group. These findings suggest that NUC-PLGA-NPs hold great promise for sustained and controlled drug delivery with improved bioavailability to alleviating lipogenesis.
[Mh] Termos MeSH primário: Aporfinas/síntese química
Sistemas de Liberação de Medicamentos/métodos
Fígado Gorduroso/tratamento farmacológico
Ácido Láctico/síntese química
Nanopartículas/química
Ácido Poliglicólico/síntese química
[Mh] Termos MeSH secundário: Administração Oral
Animais
Aporfinas/administração & dosagem
Aporfinas/metabolismo
Fenômenos Químicos
Fígado Gorduroso/metabolismo
Células Hep G2
Seres Humanos
Ácido Láctico/administração & dosagem
Ácido Láctico/metabolismo
Masculino
Nanopartículas/administração & dosagem
Nanopartículas/metabolismo
Ácido Poliglicólico/administração & dosagem
Ácido Poliglicólico/metabolismo
Distribuição Aleatória
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aporphines); 0 (polylactic acid-polyglycolic acid copolymer); 26009-03-0 (Polyglycolic Acid); 33X04XA5AT (Lactic Acid); W26UEB90B7 (nuciferine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170207
[St] Status:MEDLINE
[do] DOI:10.1080/10717544.2016.1261381


  8 / 913 MEDLINE  
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[PMID]:27976592
[Au] Autor:Dary C; Bun SS; Herbette G; Mabrouki F; Bun H; Kim S; Jabbour F; Hul S; Baghdikian B; Ollivier E
[Ad] Endereço:a Laboratory of Pharmacognosy and Ethnopharmacology, Faculty of Pharmacy , Aix Marseille Univ, UMR-MD3 , Marseille , France.
[Ti] Título:Chemical profiling of the tuber of Stephania cambodica Gagnep. (Menispermaceae) and analytical control by UHPLC-DAD.
[So] Source:Nat Prod Res;31(7):802-809, 2017 Apr.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new aporphine glycoside (1), named 'angkorwatine', and eight known alkaloids: oblongine (2), stepharine (3), asimilobine-ß-d-glucopyranoside (4), isocorydine (5), tetrahydropalmatine (THP) (6), jatrorrhizine (7), palmatine (PAL) (8), and roemerine (ROE) (9) were simultaneously isolated from the tuber of Stephania cambodica. The development and validation of UHPLC-DAD method was carried out for the quantification of marker compounds (PAL, ROE, THP) of S. cambodica. In addition to good selectivity and linearity (r > 0.997), trueness, precision, and accuracy of the method did not exceed the acceptance limit of ±10% for ROE, THP and ±20% for PAL. Consequently, this method is able to provide accurate results between 1.39-4.18 µg/mL, 2.01-30.72 µg/mL, and 4.29-64.42 µg/mL for PAL, ROE, and THP, respectively. This study shows that the validated UHPLC method is a rapid, innovative and effective analytical approach to control quality of tubers of S. cambodica and to regulate the usage of this plant in traditional medicine.
[Mh] Termos MeSH primário: Menispermaceae/química
Tubérculos/química
Stephania/química
[Mh] Termos MeSH secundário: Alcaloides/química
Aporfinas
Alcaloides de Berberina
Cromatografia Líquida de Alta Pressão
Isoquinolinas
Espectroscopia de Ressonância Magnética
Reprodutibilidade dos Testes
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Aporphines); 0 (Berberine Alkaloids); 0 (Isoquinolines); 13NS2KTD6H (aporphine); 2810-21-1 (stepharine); 3B5E87JEOX (isocorydine); 3X69CO5I79 (tetrahydropalmatine); 548-08-3 (roemerine); 60008-01-7 (oblongine); 6871-21-2 (asimilobine); G50C034217 (palmatine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2016.1247077


  9 / 913 MEDLINE  
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[PMID]:27209587
[Au] Autor:Xue B; Zhao Y; Su J; Miao Q; Miao P; Chen N; Wang Z; Zhang Y; Ma S
[Ad] Endereço:School of Chinese Materia Medica, Beijing University of Chinese Medicine, 6 Zhonghuan South Road, Wangjing, Chaoyang District, Beijing, 100102, China.
[Ti] Título:In Vitro Intestinal Absorption and Metabolism of Magnoflorine and its Potential Interaction in Coptidis Rhizoma Decoction in Rat.
[So] Source:Eur J Drug Metab Pharmacokinet;42(2):281-293, 2017 Apr.
[Is] ISSN:2107-0180
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND OBJECTIVES: In our previous studies, it was found that there existed pharmacokinetic interactions between magnoflorine and the rest of the ingredients in Coptidis Rhizoma. In this study, the pharmacokinetic interaction mechanism of magnoflorine with the rest of the components in Coptidis Rhizoma was researched based on the intestinal absorption and metabolism characteristics. METHODS: The absorption characteristics of magnoflorine in each rat intestinal segments were evaluated by non-everted intestinal sac model. To identify the metabolites of magnoflorine, the acceptor solutions of each intestinal segment at 120 min were analyzed by HPLC-LTQ-Orbitrap MS. RESULTS: The accumulative absorption (Q), the absorption rate (J) and the apparent permeability coefficient (P ) of magnoflorine were increased in duodenum, jejunum, ileum and colon of the Coptidis Rhizoma group as compared to the magnoflorine group, but there was no statistical difference between the two groups (P > 0.05). Four phase I metabolites of magnoflorine were identified in intestinal acceptor solutions of pure compound, while eight metabolites were detected in that of Coptidis Rhizoma decoction including six phase I metabolites and two phase II metabolic products. CONCLUSIONS: It was shown that the rest of the ingredients in Coptidis Rhizoma accelerated the absorption of magnoflorine weakly and promoted the metabolism of magnoflorine in the gut. The effects of other processes in the pharmacokinetics should be further evaluated.
[Mh] Termos MeSH primário: Aporfinas/farmacocinética
Medicamentos de Ervas Chinesas/química
Absorção Intestinal
Intestinos/metabolismo
[Mh] Termos MeSH secundário: Animais
Aporfinas/isolamento & purificação
Cromatografia Líquida de Alta Pressão/métodos
Interações Medicamentosas
Masculino
Espectrometria de Massas/métodos
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Aporphines); 0 (Coptidis rhizoma extract); 0 (Drugs, Chinese Herbal); NI8K6962K4 (magnoflorine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160523
[St] Status:MEDLINE
[do] DOI:10.1007/s13318-016-0344-3


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Fotocópia
[PMID]:28006931
[Au] Autor:Cermanova J; Prasnicka A; Dolezelova E; Rozkydalova L; Hroch M; Chládek J; Tomsik P; Kloeting I; Micuda S
[Ad] Endereço:Department of Pharmacology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic. micuda@lfhk.cuni.cz.
[Ti] Título:Pharmacokinetics of boldine in control and Mrp2-deficient rats.
[So] Source:Physiol Res;65(Supplementum 4):S489-S497, 2016 Dec 21.
[Is] ISSN:1802-9973
[Cp] País de publicação:Czech Republic
[La] Idioma:eng
[Ab] Resumo:The aim of the present study was to describe the currently poorly understood pharmacokinetics (PK) of boldine in control rats (LW, Lewis rats), and Mrp2 transporter-deficient rats (TR(-)). Animals from the LW and TR(-) groups underwent a bolus dose study with 10 mg/kg of boldine applied either orally or intravenously in order to evaluate the major PK parameters. The TR(-) rats demonstrated significantly reduced total clearance with prolonged biological half-life (LW 12+/-4.6 versus TR(-) 20+/-4.4 min), decreased volume of distribution (LW 3.2+/-0.4 l/kg versus TR(-) 2.4+/-0.4 l/kg) and reduced bioavailability (LW 7 % versus TR(-) 4.5 %). Another set of LW and TR(-) rats were used for a clearance study with continuous intravenous administration of boldine. The LW rats showed that biliary and renal clearance formed less than 2 % of the total clearance of boldine. The treatment of samples with beta-glucuronidase showed at least a 38 % contribution of conjugation reactions to the overall clearance of boldine. The TR(-) rats demonstrated reduced biliary clearance of boldine and its conjugates, which was partly compensated by their increased renal clearance. In conclusion, this study presents the PK parameters of boldine and shows the importance of the Mrp2 transporter and conjugation reactions in the elimination of the compound.
[Mh] Termos MeSH primário: Transportadores de Cassetes de Ligação de ATP/deficiência
Transportadores de Cassetes de Ligação de ATP/metabolismo
Anti-Inflamatórios não Esteroides/farmacocinética
Aporfinas/farmacocinética
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios não Esteroides/sangue
Aporfinas/sangue
Ratos
Ratos Endogâmicos Lew
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Abcc2 protein, rat); 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Aporphines); 8I91GE2769 (boldine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170523
[Lr] Data última revisão:
170523
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161224
[St] Status:MEDLINE



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