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Pesquisa : D03.132.760.769 [Categoria DeCS]
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[PMID]:28576466
[Au] Autor:Glynn A; Igra AM; Sand S; Ilbäck NG; Hellenäs KE; Rosén J; Aspenström-Fagerlund B
[Ad] Endereço:Swedish National Food Agency, PO Box 622, SE-751 26 Uppsala, Sweden. Electronic address: anders.glynn@slv.se.
[Ti] Título:Are additive effects of dietary surfactants on intestinal tight junction integrity an overlooked human health risk? - A mixture study on Caco-2 monolayers.
[So] Source:Food Chem Toxicol;106(Pt A):314-323, 2017 Aug.
[Is] ISSN:1873-6351
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Surfactants may cause dysfunction of intestinal tight junctions (TJs), which is a common feature of intestinal autoimmune diseases. Effects of dietary surfactants on TJ integrity, measured as trans-epithelial resistance (TEER), were studied in Caco-2 cell monolayers. Cytotoxicity was assessed as apical LDH leakage. Monolayers were apically exposed for 60 min to the dietary surfactants solanine and chaconine (SC, potato glycoalkaloids, 0-0.25 mM), perfluorooctane sulfonic acid (PFOS, industrial contaminant, 0-0.8 mM), and sucrose monolaurate (SML, food emulsifier E 473, 0-2.0 mM) separately and as a mixture. Dose-response modelling of TEER EC showed that SC were 2.7- and 12-fold more potent than PFOS and SML, respectively. The mixture was composed of 1 molar unit SC, 2.7 units PFOS and 12 units SML ("SC TEER equivalent" proportions 1:1:1). Mixture exposure (0-0.05 mM SC equivalents) dose-response modelling suggested additive action on TJ integrity. Increasing SC and SML concentrations caused increased LDH leakage, but PFOS decreased LDH leakage at intermediate exposure concentrations. In the mixture PFOS appeared to protect from extensive SC- and SML-induced LDH leakage. Complex mixtures of surfactants in food may act additively on intestinal TJ integrity, which should be considered in risk assessment of emulsifier authorisation for use in food production.
[Mh] Termos MeSH primário: Aditivos Alimentares/efeitos adversos
Intestinos/efeitos dos fármacos
Extratos Vegetais/efeitos adversos
Tensoativos/efeitos adversos
Junções Íntimas/efeitos dos fármacos
[Mh] Termos MeSH secundário: Ácidos Alcanossulfônicos/efeitos adversos
Ácidos Alcanossulfônicos/farmacologia
Células CACO-2
Fluorcarbonetos/efeitos adversos
Fluorcarbonetos/farmacologia
Aditivos Alimentares/farmacologia
Seres Humanos
L-Lactato Desidrogenase/metabolismo
Extratos Vegetais/farmacologia
Solanina/efeitos adversos
Solanina/farmacologia
Solanum tuberosum/química
Sacarose/efeitos adversos
Sacarose/análogos & derivados
Sacarose/farmacologia
Tensoativos/farmacologia
Junções Íntimas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkanesulfonic Acids); 0 (Fluorocarbons); 0 (Food Additives); 0 (Plant Extracts); 0 (Surface-Active Agents); 05Q7CD0E49 (sucrose monolaurate); 20562-02-1 (Solanine); 57-50-1 (Sucrose); 9H2MAI21CL (perfluorooctane sulfonic acid); EC 1.1.1.27 (L-Lactate Dehydrogenase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170604
[St] Status:MEDLINE


  2 / 208 MEDLINE  
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[PMID]:27855898
[Au] Autor:Koffi GY; Remaud-Simeon M; Due AE; Combes D
[Ad] Endereço:Université de Toulouse; INSA, UPS, INP, LISBP, 135 Avenue de Rangueil, F-31077 Toulouse, France; CNRS, UMR 5504, F-31400 Toulouse, France; INRA, UMR 792 Ingénierie des Systèmes Biologiques et des Procédés, F-31400 Toulouse, France; Université Nangui Abrogoua Abidjan, Côte d'Ivoire.
[Ti] Título:Isolation and chemoenzymatic treatment of glycoalkaloids from green, sprouting and rotting Solanum tuberosum potatoes for solanidine recovery.
[So] Source:Food Chem;220:257-265, 2017 Apr 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The estimation of glycoalkaloids in the flesh of different types of decayed potatoes was evaluated. The results showed that turned green and also sprouting or rotting potato flesh contain high amounts of toxic solanine and chaconine, exceeding by 2-5-fold the recommended limit, and ranging from 2578±86mg/kg to 5063±230mg/kg of dry weight potato flesh. For safety consideration, these decayed potatoes should be systematically set aside. To avoid a net economic loss and encourage the removal of this hazardous food, a recycling process was investigated to generate added-value compounds from the toxic glycoalkaloids. A simple chemo-enzymatic protocol comprising a partial acidic hydrolysis followed by an enzymatic treatment with the ß-glycosidase from Periplaneta americana allowed the efficient conversion of α-chaconine to solanidine.
[Mh] Termos MeSH primário: Diosgenina/análise
Solanum tuberosum/química
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão
Concentração de Íons de Hidrogênio
Periplaneta/enzimologia
Solanina/análogos & derivados
Solanina/química
beta-Glucosidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
20562-02-1 (Solanine); 20562-03-2 (alpha-chaconine); EC 3.2.1.21 (beta-Glucosidase); K49P2K8WLX (Diosgenin); W7801OHM8B (solanidine)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170110
[Lr] Data última revisão:
170110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE


  3 / 208 MEDLINE  
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[PMID]:27536892
[Au] Autor:Wang Y; Wu J; Guo W; Sun Q; Chen X; Zang W; Dong Z; Zhao G
[Ad] Endereço:School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
[Ti] Título:α-Solanine Modulates the Radiosensitivity of Esophageal Cancer Cells by Inducing MicroRNA 138 Expression.
[So] Source:Cell Physiol Biochem;39(3):996-1010, 2016.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Esophageal cancer (EC) is one of the most common malignant tumors in the world. Due to difficulties with performing the operation, most patients choose to have palliative treatment instead. Radiotherapy is one of the main palliative treatments of EC. However, the clinical efficacy of radiotherapy is not satisfactory α-Solanine is a bioactive component of steroidal glycoalkaloids which has been demonstrated to exhibit anti-metastasis activity in different cancers. In the present study, we determined the effect of α-solanine on the radiosensitivity of EC cells and priliminarily explored the underlying molecular mechanisms. METHODS: Cell Counting Kit-8 (CCK-8) assay was conducted to found the cytotoxic effect of α-solanine on EC cells. CCK-8 assay and colony-forming survival assays were performed to explore the effect of α-solanine on cell viability and proliferation of EC cells after irradiation. Immunofluorescence and comet assays were used to detect the effect of α-solanine on DNA repair capacity of EC cells after irradiation. The flow cytometry (FCM) and Hoechst/PI staining were conductd to study the effect of α-solanine on apoptosis of EC cells after irradiation. RESULTS: The cytotoxic effect of α-solanine to EC cells was dose-dependent. The results of CCK-8, colony-forming survival assay, immunofluorescence, comet assay, FCM and Hoechst/PI staining showed that α-solanine could enhance the radiosensitivity of EC cells. α-Solanine could downregulate Survivin expression level by upregulating miR-138 expression in EC cells. Upregulation of miR-138 and knock down Survivin both enhanced the radiosensitivity of EC cells. Moreover, Survivin could restore the effect of α-solanine and miR-138 on radiosensitivity of EC cells. CONCLUSIONS: α-solanine could enhance the radiosensitivity of esophageal cancer cells by inducing microRNA-138 expression, and probably be an effective radiosensitizer in treating EC.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Células Epiteliais/efeitos dos fármacos
Regulação Neoplásica da Expressão Gênica
MicroRNAs/agonistas
Radiossensibilizantes/farmacologia
Solanina/farmacologia
[Mh] Termos MeSH secundário: Apoptose/efeitos dos fármacos
Apoptose/efeitos da radiação
Sequência de Bases
Sítios de Ligação
Contagem de Células
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Proliferação Celular/efeitos da radiação
Sobrevivência Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos da radiação
Ensaio Cometa
Relação Dose-Resposta a Droga
Células Epiteliais/metabolismo
Células Epiteliais/patologia
Células Epiteliais/efeitos da radiação
Esôfago/efeitos dos fármacos
Esôfago/metabolismo
Esôfago/patologia
Esôfago/efeitos da radiação
Raios gama
Seres Humanos
Proteínas Inibidoras de Apoptose/antagonistas & inibidores
Proteínas Inibidoras de Apoptose/genética
Proteínas Inibidoras de Apoptose/metabolismo
MicroRNAs/genética
MicroRNAs/metabolismo
Tolerância a Radiação/efeitos dos fármacos
Tolerância a Radiação/genética
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (BIRC5 protein, human); 0 (Inhibitor of Apoptosis Proteins); 0 (MIRN138 microRNA, human); 0 (MicroRNAs); 0 (Radiation-Sensitizing Agents); 0 (alpha-solanine); 20562-02-1 (Solanine)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170202
[Lr] Data última revisão:
170202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160819
[St] Status:MEDLINE
[do] DOI:10.1159/000447807


  4 / 208 MEDLINE  
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[PMID]:27307258
[Au] Autor:Umemoto N; Nakayasu M; Ohyama K; Yotsu-Yamashita M; Mizutani M; Seki H; Saito K; Muranaka T
[Ad] Endereço:Central Laboratories for Key Technologies, Kirin Co., Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan (N.U.); RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan (N.U., K.O., K.S.); Graduate School of Agricultural Science, Kobe University, Nada-ku, Kobe, Hyo
[Ti] Título:Two Cytochrome P450 Monooxygenases Catalyze Early Hydroxylation Steps in the Potato Steroid Glycoalkaloid Biosynthetic Pathway.
[So] Source:Plant Physiol;171(4):2458-67, 2016 08.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:α-Solanine and α-chaconine, steroidal glycoalkaloids (SGAs) found in potato (Solanum tuberosum), are among the best-known secondary metabolites in food crops. At low concentrations in potato tubers, SGAs are distasteful; however, at high concentrations, SGAs are harmful to humans and animals. Here, we show that POTATO GLYCOALKALOID BIOSYNTHESIS1 (PGA1) and PGA2, two genes that encode cytochrome P450 monooxygenases (CYP72A208 and CYP72A188), are involved in the SGA biosynthetic pathway, respectively. The knockdown plants of either PGA1 or PGA2 contained very little SGA, yet vegetative growth and tuber production were not affected. Analyzing metabolites that accumulated in the plants and produced by in vitro enzyme assays revealed that PGA1 and PGA2 catalyzed the 26- and 22-hydroxylation steps, respectively, in the SGA biosynthetic pathway. The PGA-knockdown plants had two unique phenotypic characteristics: The plants were sterile and tubers of these knockdown plants did not sprout during storage. Functional analyses of PGA1 and PGA2 have provided clues for controlling both potato glycoalkaloid biosynthesis and tuber sprouting, two traits that can significantly impact potato breeding and the industry.
[Mh] Termos MeSH primário: Sistema Enzimático do Citocromo P-450/metabolismo
Solanina/análogos & derivados
Solanum tuberosum/enzimologia
[Mh] Termos MeSH secundário: Vias Biossintéticas
Cruzamento
Produtos Agrícolas
Sistema Enzimático do Citocromo P-450/genética
Inativação Gênica
Hidroxilação
Fenótipo
Fitosteróis/química
Fitosteróis/metabolismo
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Tubérculos/enzimologia
Tubérculos/genética
Tubérculos/crescimento & desenvolvimento
Solanina/química
Solanina/metabolismo
Solanum tuberosum/genética
Solanum tuberosum/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Phytosterols); 0 (Plant Proteins); 0 (alpha-solanine); 20562-02-1 (Solanine); 20562-03-2 (alpha-chaconine); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160617
[St] Status:MEDLINE
[do] DOI:10.1104/pp.16.00137


  5 / 208 MEDLINE  
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[PMID]:27222182
[Au] Autor:Zhong WF; Liu SP; Pan B; Tang ZF; Zhong JG; Zhou FJ
[Ad] Endereço:Department of Urology, Sun Yat-sen Cancer Center, Guangzhou 510060, China.E-mail: Zhongweifengzwf@126.com.
[Ti] Título:[Solanine inhibits prostate cancer Du145 xenograft growth in nude mice by inducing cell cycle arrest in G1/S phase].
[So] Source:Nan Fang Yi Ke Da Xue Xue Bao;36(5):665-70, 2016 May.
[Is] ISSN:1673-4254
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To investigate the effect of solanine on the growth of human prostate cancer cell xenograft in nude mice. METHODS: Human prostate cancer Du145 cells were injected into the subcutaneous layers on the back of nude mice. After a week, the mice bearing subcutaneous tumor graft were randomly divided into solanine treatment group and saline control group for treatment for 3 weeks. The tumor grafts were then harvested to evaluate the inhibition rate. The mRNA and protein expressions of cell cycle-related genes in the tumors were detected by qRT-PCR and Western blotting, respectively, and tumor cell apoptosis was detected using TUNEL method. RESULTS: The tumor growth rate in solanine-treated group was significantly slower than that in the control group (P<0.01). The mRNA and protein expressions of C-myc, cyclin D1, cyclin E1, CDK2, CDK4 and CDK6 were significantly inhibited by solanine. Solanine significantly up-regulated p21 mRNA and protein expression in the tumors and induced a higher apoptosis rate of the tumor cells than saline (P<0.01). CONCLUSION: The tumor-inhibition effect of solanine is probably mediated by regulating the expressions of genes related with G1/S cell cycle arrest and cell apoptosis.
[Mh] Termos MeSH primário: Pontos de Checagem da Fase G1 do Ciclo Celular
Transplante de Neoplasias/patologia
Neoplasias da Próstata/patologia
Solanina/farmacologia
[Mh] Termos MeSH secundário: Animais
Apoptose
Quinases Ciclina-Dependentes/metabolismo
Ciclinas/metabolismo
Seres Humanos
Masculino
Camundongos
Camundongos Nus
Neoplasias da Próstata/tratamento farmacológico
Fase S
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclins); 20562-02-1 (Solanine); EC 2.7.11.22 (Cyclin-Dependent Kinases)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:160525
[Lr] Data última revisão:
160525
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160526
[St] Status:MEDLINE


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[PMID]:27213896
[Au] Autor:Ventrella E; Adamski Z; Chudzinska E; Miadowicz-Kobielska M; Marciniak P; Büyükgüzel E; Büyükgüzel K; Erdem M; Falabella P; Scrano L; Bufo SA
[Ad] Endereço:Department of Sciences, University of Basilicata, Potenza, Italy.
[Ti] Título:Solanum tuberosum and Lycopersicon esculentum Leaf Extracts and Single Metabolites Affect Development and Reproduction of Drosophila melanogaster.
[So] Source:PLoS One;11(5):e0155958, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glycoalkaloids are secondary metabolites commonly found in Solanaceae plants. They have anti-bacterial, anti-fungal and insecticidal activities. In the present study we examine the effects of potato and tomato leaf extracts and their main components, the glycoalkaloids α-solanine, α-chaconine and α-tomatine, on development and reproduction of Drosophila melanogaster wild-type flies at different stages. Parental generation was exposed to five different concentrations of tested substances. The effects were examined also on the next, non-exposed generation. In the first (exposed) generation, addition of each extract reduced the number of organisms reaching the pupal and imaginal stages. Parent insects exposed to extracts and metabolites individually applied showed faster development. However, the effect was weaker in case of single metabolites than in case of exposure to extracts. An increase of developmental rate was also observed in the next, non-exposed generation. The imagoes of both generations exposed to extracts and pure metabolites showed some anomalies in body size and malformations, such as deformed wings and abdomens, smaller black abdominal zone. Our results further support the current idea that Solanaceae can be an impressive source of molecules, which could efficaciously be used in crop protection, as natural extract or in formulation of single pure metabolites in sustainable agriculture.
[Mh] Termos MeSH primário: Drosophila melanogaster/fisiologia
Lycopersicon esculentum/química
Extratos Vegetais/farmacologia
Reprodução/efeitos dos fármacos
Solanum tuberosum/química
[Mh] Termos MeSH secundário: Animais
Tamanho Corporal/efeitos dos fármacos
Drosophila melanogaster/efeitos dos fármacos
Drosophila melanogaster/crescimento & desenvolvimento
Feminino
Masculino
Controle Biológico de Vetores
Extratos Vegetais/química
Folhas de Planta/química
Solanina/análogos & derivados
Solanina/farmacologia
Tomatina/análogos & derivados
Tomatina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (alpha-solanine); 0 (alpha-tomatine); 20562-02-1 (Solanine); 20562-03-2 (alpha-chaconine); 31U6547O08 (Tomatine)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160524
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0155958


  7 / 208 MEDLINE  
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[PMID]:27023503
[Au] Autor:Hossain MB; Brunton NP; Rai DK
[Ad] Endereço:Department of Food Biosciences, Teagasc Food Research Centre, Ashtown, D15 DY05 Dublin, Ireland. mohammad.hossain@teagasc.ie.
[Ti] Título:Effect of Drying Methods on the Steroidal Alkaloid Content of Potato Peels, Shoots and Berries.
[So] Source:Molecules;21(4):403, 2016 Mar 25.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The present study has found that dried potato samples yielded significantly higher levels of steroidal alkaloids such as α-solanine and α-chaconine than the corresponding fresh samples, as determined by the UPLC-MS/MS technique. Among the drying techniques used, air drying had the highest effect on steroidal alkaloid contents, followed by freeze drying and vacuum oven drying. There was no significant difference between the freeze dried and vacuum oven dried samples in their α-chaconine contents. However, freeze dried potato shoots and berries had significantly higher α-solanine contents (825 µg/g dry weight (DW) in shoots and 2453 µg/g DW in berries) than the vacuum oven dried ones (325 µg/g dry weight (DW) in shoots and 2080 µg/g DW in berries). The kinetics of steroidal alkaloid contents of potato shoots during air drying were monitored over a period of 21 days. Both α-solanine and α-chaconine content increased to their maximum values, 875 µg/g DW and 3385 µg/g DW, respectively, after 7 days of drying. The steroidal alkaloid contents of the shoots decreased significantly at day 9, and then remained unchanged until day 21. In line with the potato shoots, air dried potato tuber peels also had higher steroidal alkaloid content than the freeze dried and vacuum oven dried samples. However, a significant decrease of steroidal alkaloid content was observed in air dried potato berries, possibly due to degradation during slicing of the whole berries prior to air drying. Remarkable variation in steroidal alkaloid contents among different tissue types of potato plants was observed with the potato flowers having the highest content.
[Mh] Termos MeSH primário: Alcaloides/isolamento & purificação
Fitosteróis/isolamento & purificação
Solanina/análogos & derivados
[Mh] Termos MeSH secundário: Alcaloides/química
Cromatografia Líquida de Alta Pressão
Liofilização
Frutas/química
Fitosteróis/química
Brotos de Planta/química
Solanina/química
Solanina/isolamento & purificação
Solanum tuberosum/química
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Phytosterols); 0 (alpha-solanine); 20562-02-1 (Solanine); 20562-03-2 (alpha-chaconine)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160330
[St] Status:MEDLINE


  8 / 208 MEDLINE  
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[PMID]:26931732
[Au] Autor:Shin JS; Lee KG; Lee HH; Lee HJ; An HJ; Nam JH; Jang DS; Lee KT
[Ad] Endereço:Department of Pharmaceutical Biochemistry, Kyung Hee University, Seoul, Republic of Korea.
[Ti] Título:α-Solanine Isolated From Solanum Tuberosum L. cv Jayoung Abrogates LPS-Induced Inflammatory Responses Via NF-κB Inactivation in RAW 264.7 Macrophages and Endotoxin-Induced Shock Model in Mice.
[So] Source:J Cell Biochem;117(10):2327-39, 2016 Oct.
[Is] ISSN:1097-4644
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:α-Solanine, a trisaccharide glycoalkaloid, has been reported to possess anti-cancer effects. In this study, we investigated the anti-inflammatory effects of α-solanine isolated from "Jayoung" a dark purple-fleshed potato by examining its in vitro inhibitory effects on inducible nitric-oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokines in LPS-induced RAW 264.7 macrophages and its in vivo effects on LPS-induced septic shock in a mouse model. α-Solanine suppressed the expression of iNOS and COX-2 both at protein and mRNA levels and consequently inhibited nitric oxide (NO) and prostaglandin E2 (PGE2 ) production in LPS-induced RAW 264.7 macrophages. α-Solanine also reduced the production and mRNA expression of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interleukin-1ß (IL-1ß) induced by LPS. Furthermore, molecular mechanism studies indicated that α-solanine inhibited LPS-induced activation of nuclear factor-κB (NF-κB) by reducing nuclear translocation of p65, degradation of inhibitory κBα (IκBα), and phosphorylation of IκB kinaseα/ß (IKKα/ß). In an in vivo experiment of LPS-induced endotoxemia, treatment with α-solanine suppressed mRNA expressions of iNOS, COX-2, IL-6, TNF-α, and IL-1ß, and the activation of NF-κB in liver. Importantly, α-solanine increased the survival rate of mice in LPS-induced endotoxemia and polymicrobial sepsis models. Taken together, our data suggest that the α-solanine may be a promising therapeutic against inflammatory diseases by inhibiting the NF-κB signaling pathway. J. Cell. Biochem. 117: 2327-2339, 2016. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Colite/prevenção & controle
Inflamação/prevenção & controle
Macrófagos/efeitos dos fármacos
NF-kappa B/metabolismo
Choque Séptico/prevenção & controle
Solanina/farmacologia
Solanum tuberosum/química
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Western Blotting
Proliferação Celular/efeitos dos fármacos
Células Cultivadas
Colite/induzido quimicamente
Colite/metabolismo
Citocinas/genética
Citocinas/metabolismo
Modelos Animais de Doenças
Inflamação/induzido quimicamente
Inflamação/metabolismo
Mediadores da Inflamação/metabolismo
Lipopolissacarídeos/toxicidade
Macrófagos/citologia
Macrófagos/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
NF-kappa B/genética
Óxido Nítrico/metabolismo
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Choque Séptico/induzido quimicamente
Choque Séptico/metabolismo
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Inflammation Mediators); 0 (Lipopolysaccharides); 0 (NF-kappa B); 0 (RNA, Messenger); 0 (alpha-solanine); 20562-02-1 (Solanine); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160303
[St] Status:MEDLINE
[do] DOI:10.1002/jcb.25530


  9 / 208 MEDLINE  
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[PMID]:26854999
[Au] Autor:Mohsenikia M; Farhangi B; Alizadeh AM; Khodayari H; Khodayari S; Khori V; Arjmand Abbassi Y; Vesovic M; Soleymani A; Najafi F
[Ad] Endereço:Dezful University of Medical Sciences, Dezful, Iran.
[Ti] Título:Therapeutic effects of dendrosomal solanine on a metastatic breast tumor.
[So] Source:Life Sci;148:260-7, 2016 Mar 01.
[Is] ISSN:1879-0631
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:AIMS: Our previous studies showed that alpha-solanine can inhibit tumor growth in cell culture and animal models of breast cancer. However, solanine is insoluble in common solvents; therefore, we developed a special nanoparticle with high-capacity solubility. The present study is aimed to deliberate the therapeutic effects of dendrosomal solanine (DNS) on a metastatic breast tumor in vitro and in vivo. MAIN METHODS: After DNS preparation and dosing procedures, forty-five mice were equally divided into five groups to investigate the anti-metastatic effects of DNS on mammary tumor-bearing mice. KEY FINDINGS: Compared to solanine, DNS significantly suppressed the proliferation of 4 T1 cells in a dose- and time-dependent manner. DNS showed a remarkable safety rate of up to 10mg/kg. A significant decrease in white blood-cell count was seen at 20mg/kg DNS in comparison with control animals. Mice treated with DNS had smaller tumor volume (mm(3)) in comparison with control and solanine groups. Moreover, the incidence of the breast tumor metastases was about 67% in the control animals, where as solanine and DNS 1mg/kg were about 22% and 0%, respectively. Furthermore, the number of metastases per mouse varied from one to three. The tissues of tumor, brain, liver, spleen, and lung showed higher expression levels of Bcl-2 but lower expression levels of Bax, MMP-2, MMP-9, mTOR, and Akt in DNS-treated mice than control and solanine groups. SIGNIFICANCE: The findings suggest that DNS has a more impactful therapeutic effect than solanine on 4 T1-induced breast tumorigenesis via influencing the tissue microenvironment.
[Mh] Termos MeSH primário: Neoplasias da Mama/tratamento farmacológico
Neoplasias da Mama/patologia
Portadores de Fármacos/administração & dosagem
Nanopartículas/administração & dosagem
Solanina/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Feminino
Camundongos
Camundongos Endogâmicos BALB C
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Drug Carriers); 20562-02-1 (Solanine)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:160314
[Lr] Data última revisão:
160314
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160209
[St] Status:MEDLINE


  10 / 208 MEDLINE  
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[PMID]:26830588
[Au] Autor:Shepherd LV; Hackett CA; Alexander CJ; McNicol JW; Sungurtas JA; McRae D; McCue KF; Belknap WR; Davies HV
[Ad] Endereço:Environmental and Biochemical Sciences Group, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, United Kingdom. Electronic address: louise.shepherd@hutton.ac.uk.
[Ti] Título:Impact of light-exposure on the metabolite balance of transgenic potato tubers with modified glycoalkaloid biosynthesis.
[So] Source:Food Chem;200:263-73, 2016 Jun 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Metabolite profiling (liquid chromatography-mass spectrometry (LC-MS) and gas chromatography (GC-MS)) was used to assess the impact of light on the composition of transgenic potato (Solanum tuberosum L. cv. Desirée) with reduced glycoalkaloid content via the down-regulation of the SGT1 gene. Transgenic tubers exhibited an almost complete knock-out of α-solanine production and light had little impact on its accumulation. Levels of α-chaconine increased significantly in the peel of both the control and transgenic lines when exposed to light, particularly in the transgenic line. Major differences in metabolite profiles existed between outer and inner tuber tissues, and between light and dark-treated tubers. Many of the light-induced changes are explicable in terms of pathways known to be affected by stress responses. The impact of transgenesis on profiles was much less than that of tissue type or light and most differences were explicable in terms of the modification to the glycoalkaloid pathway.
[Mh] Termos MeSH primário: Tubérculos/metabolismo
Plantas Geneticamente Modificadas/metabolismo
Alcaloides de Solanáceas/biossíntese
Solanum tuberosum/metabolismo
[Mh] Termos MeSH secundário: Clorofila/análise
Cromatografia Líquida
Cromatografia Gasosa-Espectrometria de Massas
Luz
Espectrometria de Massas
Solanina/análogos & derivados
Solanina/análise
Solanum tuberosum/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Solanaceous Alkaloids); 0 (alpha-solanine); 1406-65-1 (Chlorophyll); 20562-02-1 (Solanine); 20562-03-2 (alpha-chaconine)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160202
[Lr] Data última revisão:
160202
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160203
[St] Status:MEDLINE



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