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Pesquisa : D03.132.920.256 [Categoria DeCS]
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[PMID]:28415020
[Au] Autor:Li J; Li D; Pan Y; Hu JH; Huang W; Wang ZZ; Xiao W; Wang Y
[Ad] Endereço:School of pharmacy, China Pharmaceutical University, NO.24 Tongjia Rd, Nanjing 210009, China; State Key Lab of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China.
[Ti] Título:Simultaneous determination of ten bioactive constituents of Sanjie Zhentong Capsule in rat plasma by ultra-high-performance liquid chromatography tandem mass spectrometry and its application to a pharmacokinetic study.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1054:20-26, 2017 Jun 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Sanjie Zhentong capsule, a well-known traditional Chinese medicine prescription, are used for the treatment of endometriosis-related diseases. In this study, a simple, rapid and sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous determination of ten bioactive constituents, including peimine, peiminine, peimisine, loureirin A, loureirin B, 7,4'-dihydroxyflavone, pterostilbene, ginsenoside Rg1, ginsenoside Rb1, and notoginsenoside R1 in rat plasma after oral administration of Sanjie Zhentong capsule. The sample preparations for protein removal was accomplished using a simple methanol precipitation method. The analytes were completely separated from the endogenous compounds on an Agilent Poroshell 120 SB-C18 column (4.6mm×150mm, 2.7µm) using an isocratic elution with methanol - 0.1% formic acid aqueous (4/1, v/v) as a mobile phase. The single-run analysis time was as short as 14.0min. The inter-day and intra-day precision of the quality control samples exhibited relative standard deviations (RSD) <9.5% and the accuracy values ranged from -8.6% to 15.0%. The lower limits of quantification (LLOQ) were 10, 10, 10, 10, 10, 10, 5, 10, 10 and 20ng/mL for peimine, peiminine, peimisine, loureirin A, loureirin B, 7,4'-dihydroxyflavone, pterostilbene, ginsenoside Rg1, ginsenoside Rb1, notoginsenoside R1, respectively. The analytical method was successfully applied to a pharmacokinetic study of the multi-components after oral administration of Sanjie Zhentong Capsule in rats.
[Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/farmacocinética
[Mh] Termos MeSH secundário: Administração Oral
Alcaloides/sangue
Animais
Cevanas/sangue
Chalconas/sangue
Cromatografia Líquida de Alta Pressão/métodos
Medicamentos de Ervas Chinesas/administração & dosagem
Feminino
Flavonas/sangue
Ginsenosídeos/sangue
Limite de Detecção
Ratos Sprague-Dawley
Resinas Vegetais/farmacocinética
Estilbenos/sangue
Espectrometria de Massas em Tandem/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (7,4'-dihydroxyflavone); 0 (Alkaloids); 0 (Cevanes); 0 (Chalcones); 0 (Drugs, Chinese Herbal); 0 (Flavones); 0 (Ginsenosides); 0 (Resins, Plant); 0 (Stilbenes); 0 (loureirin A); 19773-24-1 (peimisine); 26R60S6A5I (pterostilbene); 34QDF8UFSY (verticine); 7413S0WMH6 (ginsenoside Rb1); JKN43410XZ (peiminine); P47L69798O (loureirin B); PJ788634QY (ginsenoside Rg1); Z62692362Z (notoginsenoside R1)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE


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[PMID]:27524867
[Au] Autor:Wang D; Du Q; Li H; Wang S
[Ad] Endereço:Department of Pharmacognosy, West China College of Pharmacy, Sichuan University, No. 17, Duan 3, Renmin Nan Road, Chengdu 610041, China; Department of Pharmacognosy, Faculty of Life Sciences, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria.
[Ti] Título:The Isosteroid Alkaloid Imperialine from Bulbs of Fritillaria cirrhosa Mitigates Pulmonary Functional and Structural Impairment and Suppresses Inflammatory Response in a COPD-Like Rat Model.
[So] Source:Mediators Inflamm;2016:4192483, 2016.
[Is] ISSN:1466-1861
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chronic obstructive pulmonary disease (COPD) is the third leading cause of death in the world. Present therapies for COPD have limited effect on reducing the progression of COPD and suppressing the inflammatory response in the lung. Bulbs of Fritillaria cirrhosa D. Don (BFC) have been used in many Asian countries for a long time to treat pulmonary diseases, such as cough, expectoration, and asthma. Steroidal alkaloids are the major biological active constituents in BFC, whereby imperialine is one of the important steroidal alkaloids. So far, there are no studies reporting the effect of imperialine on COPD. In this study, we investigated the effect of imperialine on pulmonary function and structure and inflammation in a COPD-like rat model which was induced by the combination of exposure to CS and intratracheal administration of LPS. Our data show that imperialine mitigates pulmonary functional and structural impairment and suppressed inflammatory response in a COPD-like rat model by mediating expression of related cytokines in lung tissues of the COPD-like rats, such as IL-1ß, IL-6, IL-8, TNF-α, NF-κB, TGF-ß1, MMP-9, and TIMP-1.
[Mh] Termos MeSH primário: Alcaloides/farmacologia
Fritillaria/química
Inflamação/tratamento farmacológico
Pulmão/efeitos dos fármacos
Extratos Vegetais/farmacologia
Doença Pulmonar Obstrutiva Crônica/fisiopatologia
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/farmacologia
Peso Corporal
Cevanas/metabolismo
Citocinas/metabolismo
Modelos Animais de Doenças
Imuno-Histoquímica
Pulmão/metabolismo
Masculino
Raízes de Plantas/química
Doença Pulmonar Obstrutiva Crônica/tratamento farmacológico
Ratos
Ratos Wistar
Testes de Função Respiratória
Esteroides/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Anti-Inflammatory Agents); 0 (Cevanes); 0 (Cytokines); 0 (Plant Extracts); 0 (Steroids); JKN43410XZ (peiminine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160816
[St] Status:MEDLINE
[do] DOI:10.1155/2016/4192483


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[PMID]:27033404
[Au] Autor:Xu J; Zhao W; Pan L; Zhang A; Chen Q; Xu K; Lu H; Chen Y
[Ad] Endereço:Chinese Herb Medicine Division, Zhejiang Agriculture and Forestry University, 88 North Circle Road, Lin'an 311300, PR China; The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, 88 North Circle Road, Lin'an 311300, PR China.
[Ti] Título:Peimine, a main active ingredient of Fritillaria, exhibits anti-inflammatory and pain suppression properties at the cellular level.
[So] Source:Fitoterapia;111:1-6, 2016 Jun.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Fritillaria is one of the most important herbs in Chinese traditional medicine and represents an annual ï¿¥700 million industry. It is often used as an anti-inflammatory, pain relieving and antitussive medicine. However, the mechanisms of these effects are still unclear. Peimine is one of active ingredients of Fritillaria. Using the patch-clamp technique, we profiled the action of Peimine against selected ion channels stably expressed in HEK 293 cell lines. Our data indicated that Peimine was not only able to block the Nav1.7 ion channel but also preferably inhibited the Kv1.3 ion channel. Thus, the study suggested potential mechanisms of Fritillaria as a pain relieving and anti-inflammatory herb.
[Mh] Termos MeSH primário: Analgésicos/farmacologia
Anti-Inflamatórios/farmacologia
Cevanas/farmacologia
Fritillaria/química
Canal de Sódio Disparado por Voltagem NAV1.7/metabolismo
[Mh] Termos MeSH secundário: Analgésicos/química
Medicamentos de Ervas Chinesas/farmacologia
Células HEK293
Seres Humanos
Técnicas de Patch-Clamp
Plantas Medicinais/química
Bloqueadores do Canal de Sódio Disparado por Voltagem/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Anti-Inflammatory Agents); 0 (Cevanes); 0 (Drugs, Chinese Herbal); 0 (NAV1.7 Voltage-Gated Sodium Channel); 0 (SCN9A protein, human); 0 (Voltage-Gated Sodium Channel Blockers); 34QDF8UFSY (verticine)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170117
[Lr] Data última revisão:
170117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160402
[St] Status:MEDLINE


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[PMID]:26946033
[Au] Autor:Long Z; Guo Z; Acworth IN; Liu X; Jin Y; Liu X; Liu L; Liang L
[Ad] Endereço:Thermofisher Scientific Corporation, Beijing 100080, China.
[Ti] Título:A non-derivative method for the quantitative analysis of isosteroidal alkaloids from Fritillaria by high performance liquid chromatography combined with charged aerosol detection.
[So] Source:Talanta;151:239-44, 2016 May 01.
[Is] ISSN:1873-3573
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A non-derivative method was developed for the qualitative and quantitative analysis of isosteroidal alkaloids from Fritillaria thunbergii. During method development the performance of two universal detectors, the charged aerosol detector (CAD) and evaporative light scattering detector (ELSD), were evaluated. The CAD was found to be 30 to 55 times more sensitive than ELSD enabling the measurement of low levels of reference compound impurities that could not be detected by ELSD. The peak area percent of the reference compound, peimisine, obtained by CAD was 50.10%, but 91.66% by ELSD showing that CAD is suitable to estimate the presence of impurities. The CAD showed good reproducibility with overall intra- and inter-day peak area RSD values of less than 1.8% and 2.7%, respectively and had a linear dynamic range of up to 4 orders of magnitude (0.06-44mg/L) for peimine and peiminine. The optimized method was used for the quantitative analysis of peimine and peiminine from F. thunbergii.
[Mh] Termos MeSH primário: Aerossóis/análise
Alcaloides/análise
Cromatografia Líquida de Alta Pressão/métodos
Fritillaria/química
[Mh] Termos MeSH secundário: Cevanas/análise
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aerosols); 0 (Alkaloids); 0 (Cevanes); 0 (hupehenine); 19773-24-1 (peimisine); 34QDF8UFSY (verticine); JKN43410XZ (peiminine)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160307
[St] Status:MEDLINE


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[PMID]:26926170
[Au] Autor:Kim EJ; Yoon YP; Woo KW; Kim JH; Min SY; Lee HJ; Lee SK; Hong JH; Lee KR; Lee CJ
[Ad] Endereço:Department of Pediatrics, College of Korean Medicine, Dongguk University, Gyeongju, South Korea.
[Ti] Título:Verticine, ebeiedine and suchengbeisine isolated from the bulbs of Fritillaria thunbergii Miq. inhibited the gene expression and production of MUC5AC mucin from human airway epithelial cells.
[So] Source:Phytomedicine;23(2):95-104, 2016 Feb 15.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The bulb of Fritillaria thunbergii has been utilised as mucoregulators and expectorants for controlling the airway inflammatory diseases in folk medicine. HYPOTHESIS/PURPOSE: We investigated whether verticine, ebeiedine and suchengbeisine isolated from the bulbs of Fritillaria thunbergii inhibit the gene expression and production of MUC5AC mucin from human airway epithelial cells. STUDY DESIGN: Confluent NCI-H292 cells were pretreated with verticine, ebeiedine or suchengbeisine for 30 min and then stimulated with EGF, PMA or TNF-α for 24h. The MUC5AC mucin gene expression was measured by RT-PCR. Production of MUC5AC mucin protein was measured by ELISA. RESULTS: (1) Verticine, ebeiedine or suchengbeisine inhibited the expression of MUC5AC mucin gene induced by EGF, PMA or TNF-α; (2) The production of MUC5AC mucin protein induced by EGF, PMA or TNF-α were also inhibited by treatment of verticine, ebeiedine or suchengbeisine. CONCLUSION: These results suggest that verticine, ebeiedine and suchengbeisine isolated from the bulbs of Fritillaria thunbergii inhibit the gene expression and production of MUC5AC mucin, by directly acting on airway epithelial cells, and the results are consistent with the traditional use of Fritillaria thunbergii as remedy for diverse inflammatory pulmonary diseases.
[Mh] Termos MeSH primário: Alcaloides/química
Cevanas/química
Células Epiteliais/efeitos dos fármacos
Fritillaria/química
Mucina-5AC/metabolismo
Esteroides/química
[Mh] Termos MeSH secundário: Alcaloides/isolamento & purificação
Linhagem Celular Tumoral
Cevanas/isolamento & purificação
Fator de Crescimento Epidérmico/farmacologia
Células Epiteliais/metabolismo
Expressão Gênica/efeitos dos fármacos
Seres Humanos
Estrutura Molecular
Mucina-5AC/genética
Extratos Vegetais/química
Raízes de Plantas/química
Esteroides/isolamento & purificação
Fator de Necrose Tumoral alfa/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Cevanes); 0 (MUC5AC protein, human); 0 (Mucin 5AC); 0 (Plant Extracts); 0 (Steroids); 0 (Tumor Necrosis Factor-alpha); 0 (ebeiedine); 0 (suchengbeisine); 34QDF8UFSY (verticine); 62229-50-9 (Epidermal Growth Factor)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE


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[PMID]:27254925
[Au] Autor:Niu HY; Jin SS; Jia YP; Zhang XX; Yu HS; Song XB
[Ti] Título:[Comparative Analysis of Content of Four Alkaloids in Fritillaria hupehensis and Fritillaria ebeiensis var. purpurea].
[So] Source:Zhong Yao Cai;38(10):2105-8, 2015 Oct.
[Is] ISSN:1001-4454
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To establish an assay method for simultaneous determination of peimine, peiminine, peimissine and hupehenine and to make a comparative analysis of the content of four alkaloids in Fritillaria hupehensis and Fritillaria ebeiensis var. purpurea for the first time. METHODS: A Unitary C18 column(250 mm x 4.6 mm, 5 µm) was chosen with acetonitrile-water (containing 0.05% diethylamine) as mobile phase in a gradient program. The column temperature was 35 degrees C and the flow-rate was 1.0 mL/min. RESULTS: There was high content of peiminine and the content of peimissine was inferior to peiminine in Fritillaria hupehensis. Relatively speaking, peimine and hupehenine were much lower than the other two ingredients. Fritillaria ebeiensis var. purpurea also contained high levels of peiminine, the minimum content of peimine and equivalent content of peimissine comparing with Fritillaria hupehensis. In addition, it didn't contain hupehenine in Fritillaria ebeiensis var. purpurea. CONCLUSION: This method is simple and fast, and it has good separation, reproducibility and reliable results. Also, it can be used as basis for the quality evaluation of Fritillaria hupehensis and Fritillaria ebeiensis var. purpurea.
[Mh] Termos MeSH primário: Alcaloides/isolamento & purificação
Cevanas/isolamento & purificação
Fritillaria/química
Reprodutibilidade dos Testes
[Mh] Termos MeSH secundário: Fritillaria/classificação
Plantas Medicinais/química
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Cevanes); 0 (hupehenine); 19773-24-1 (peimisine); 34QDF8UFSY (verticine); JKN43410XZ (peiminine)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160604
[St] Status:MEDLINE


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[PMID]:26882613
[Au] Autor:Tang XY; Tang YX; Xu P; Zhou HY; Han L
[Ti] Título:[Effect of Peimine on ERCC1 mRNA and LRP Expressions of A549/DDP Multidrug Resistance Cell Line].
[So] Source:Zhongguo Zhong Xi Yi Jie He Za Zhi;35(12):1490-4, 2015 Dec.
[Is] ISSN:1003-5370
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:OBJECTIVE: To explore the effect of peimine on excision repair cross-complementation 1 (ERCC1) mRNA and lung resistant protein (LRP) expressions in A549/cisplatin (DDP) multidrug resistance (MDR) cell line. METHODS: Lung cancer A549/DDP cells were cultured in vitro.Cells at logarithmic growth phase were divided into 4 groups, i.e., the blank control group, the DDP group, the ligustrazine group (DDP+ligustrazine), the peimine group (DDP + peimine). After 48-h drug action, ERCC1 mRNA expression was detected by RT-PCR and LRP expression detected by cell immunofluorescence. RESULTS: There was no statistical difference in expression levels of ERCC1 mRNA and LRP between the DDP group and the blank control group (P > 0.05). Compared with the DDP group, expression levels of ERCC1 mRNA and LRP obviously decreased in the ligustrazine group and the peimine group (P < 0.05). They were obviously lower in the peimine group than in the ligustrazine group (P < 0.05). CONCLUSIONS: Peimine could reverse MDR of A549/DDP cell line. Its mechanism might be associated with down-regulating ERCC1 mRNA and LRP expression levels.
[Mh] Termos MeSH primário: Cevanas/farmacologia
Proteínas de Ligação a DNA/genética
Resistência a Medicamentos Antineoplásicos/efeitos dos fármacos
Endonucleases/genética
Proteína-1 Relacionada a Receptor de Lipoproteína de Baixa Densidade/genética
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Cisplatino
Regulação para Baixo
Resistência a Múltiplos Medicamentos
Seres Humanos
Neoplasias Pulmonares
RNA Mensageiro/metabolismo
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cevanes); 0 (DNA-Binding Proteins); 0 (LRP1 protein, human); 0 (Low Density Lipoprotein Receptor-Related Protein-1); 0 (RNA, Messenger); 34QDF8UFSY (verticine); EC 3.1.- (ERCC1 protein, human); EC 3.1.- (Endonucleases); Q20Q21Q62J (Cisplatin)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:161018
[Lr] Data última revisão:
161018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160218
[St] Status:MEDLINE


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[PMID]:26349052
[Au] Autor:Lin Q; Fu Y; Li J; Qu M; Deng L; Gong T; Zhang Z
[Ad] Endereço:Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, PR China.
[Ti] Título:A (polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer)-dispersed sustained-release tablet for imperialine to simultaneously prolong the drug release and improve the oral bioavailability.
[So] Source:Eur J Pharm Sci;79:44-52, 2015 Nov 15.
[Is] ISSN:1879-0720
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Imperialine, extracted from Bulbus Fritillariae Cirrhosae, is an efficient antitussive and expectorant medicine. However, its short half-life and stomach degradation limited imperialine from further clinical use. The current study was conducted to develop a sustained-release tablet for imperialine both to prolong absorption time and to improve the oral bioavailability of the drug. The tablets were prepared by a direct compression method formulated on optimized solid dispersion (SD) for imperialine based on polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer (Soluplus(®)) with imperialine/Soluplus(®) ratio of 1:8 (w/w). In order to obtain the optimized formulation, factors that affected the drug release were investigated by in vitro dissolution studies in the media of pH1.2, 5.8, 7.0 and 7.4. Powder X-ray diffraction and scanning electron microscope confirmed that the imperialine in SD was amorphous instead of crystalline, and still stayed amorphous even after the direct compression. And besides, pharmacokinetic study in Beagle dogs was performed to inspect the in vivo sustained release. Plasma concentration-time curves and pharmacokinetic parameters were gained. As a result, the Cmax of imperialine was one-fold reduced and Tmax was two-fold prolonged, and the mean AUC0-24 was expressed as 89.581±21.243µgh/L, which showed that the oral bioavailability of imperialine was 2.46-fold improved. Moreover, the in vitro-in vivo correlation was recommended to carry out, demonstrating the percentages of drug release in vitro were well-correlated with the absorptive fraction in vivo with the correlation coefficients above 0.9900. By mathematically modeling and moment imaging of the drug release, Peppas equation was selected as the most fitted model for the sustained-release tablets with the diffusional coefficient in the range of 0.59-0.62, indicating the release of imperialine from the sustained-release tablets was an anomalous process involving polymer swelling, drug diffusion and matrix erosion.
[Mh] Termos MeSH primário: Antitussígenos/administração & dosagem
Cevanas/administração & dosagem
Polietilenoglicóis/uso terapêutico
Polivinil/uso terapêutico
[Mh] Termos MeSH secundário: Administração Oral
Animais
Antitussígenos/farmacocinética
Disponibilidade Biológica
Cevanas/farmacocinética
Preparações de Ação Retardada
Cães
Feminino
Masculino
Comprimidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antitussive Agents); 0 (Cevanes); 0 (Delayed-Action Preparations); 0 (Polyvinyls); 0 (Tablets); 0 (polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer); 30IQX730WE (Polyethylene Glycols); JKN43410XZ (peiminine)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150909
[St] Status:MEDLINE


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[PMID]:26132855
[Au] Autor:Wu K; Mo C; Xiao H; Jiang Y; Ye B; Wang S
[Ad] Endereço:Department of Medicinal Natural Products, West China School of Pharmacy, Sichuan University, Chengdu, China.
[Ti] Título:Imperialine and Verticinone from Bulbs of Fritillaria wabuensis Inhibit Pro-inflammatory Mediators in LPS-stimulated RAW 264.7 Macrophages.
[So] Source:Planta Med;81(10):821-9, 2015 Jul.
[Is] ISSN:1439-0221
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The bulbs of plants belonging to the Fritillaria cirrhosa-group have been used as antitussive and expectorant herbs in traditional Chinese medicine for thousands of years. In this study, we isolated two isomers of verticinone and imperialine, steroidal alkaloids belonging to the cevanine group, from bulbs of Fritillaria wabuensis, which is a part of the Fritillaria cirrhosa group, and investigated their anti-inflammatory effects and relative mechanisms on lipopolysaccharide-stimulated RAW 264.7 macrophages. Our results clearly demonstrate that verticinone or imperialine could dose-dependently inhibit nitric oxide production and also suppress inducible nitric oxide synthase and cyclooxygenase-2 expressions. In addition, verticinone or imperialine suppress the production of pro-inflammatory cytokines in a dose dependent manner, such as tumor necrosis factor-α and interleukin-1ß. The effect of verticinone and imperialine on the activation of nuclear factor-kappaB was also evaluated. The phosphorylation of nuclear factor-kappaB stimulated with LPS is also down-regulated by verticinone or imperialine in a concentration dependent manner, which coincided with the inhibition of phosphorylation forms of inhibitory kappaB-α, a crucial inhibitory factor of nuclear factor-kappaB. Generally, the anti-inflammatory effects and mechanisms of verticinone and imperialine are mediated by the inhibition of the nuclear factor-kappaB activation signaling pathway. According to the results of our researches, verticinone and imperialine may present great potentials to be developed as therapeutics for inflammatory diseases.
[Mh] Termos MeSH primário: Cevanas/farmacologia
Fritillaria/química
Mediadores da Inflamação/antagonistas & inibidores
Macrófagos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios não Esteroides/química
Anti-Inflamatórios não Esteroides/farmacologia
Linhagem Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Ciclo-Oxigenase 2/metabolismo
Relação Dose-Resposta a Droga
Mediadores da Inflamação/metabolismo
Interleucina-1beta/metabolismo
Lipopolissacarídeos/farmacologia
Macrófagos/metabolismo
Camundongos
Óxido Nítrico/metabolismo
Óxido Nítrico Sintase Tipo II/metabolismo
Tubérculos/química
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Cevanes); 0 (IL1B protein, mouse); 0 (Inflammation Mediators); 0 (Interleukin-1beta); 0 (Lipopolysaccharides); 0 (Tumor Necrosis Factor-alpha); 31C4KY9ESH (Nitric Oxide); 34QDF8UFSY (verticine); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.13.39 (Nos2 protein, mouse); EC 1.14.99.- (Ptgs2 protein, mouse); EC 1.14.99.1 (Cyclooxygenase 2); JKN43410XZ (peiminine)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150702
[St] Status:MEDLINE
[do] DOI:10.1055/s-0035-1546170


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[PMID]:26121924
[Au] Autor:Lee B; Kim EY; Kim JH; Min JH; Jeong DW; Jun JY; Cho CY; Sohn Y; Jung HS
[Ad] Endereço:Department of Anatomy, College of Korean Medicine, Kyung Hee University Seoul , Republic of Korea.
[Ti] Título:Antiallergic effects of peiminine through the regulation of inflammatory mediators in HMC-1 cells.
[So] Source:Immunopharmacol Immunotoxicol;37(4):351-8, 2015.
[Is] ISSN:1532-2513
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Peiminine is the main biologically active component derived from Fritillaria ussuriensis. Peiminine was investigated in various pulmonary diseases, but its antiallergic effect and the related mechanism have not been reported yet. The present study aimed to evaluate the effect of peiminine on mast cell-mediated allergic inflammation in HMC-1 cells. The pro-inflammatory cytokine production was measured using ELISA, reverse transcription-polymerase chain reaction and nuclear factor-kappaB (NF-κB), mitogen-activated protein kinases (MAPKs) pathway activation, as determined by Western blot analysis. Peiminine inhibits the production of the pro-inflammatory cytokine, such as interleukin (IL)-6, IL-8, tumor necrosis factor-alpha (TNF-α) and IL-1beta (IL-1ß). It was shown to have inhibitory effects on MAPKs phosphorylation and NF-B expression in human mast cells (HMC)-1 using Western blot. HMC-1 cells were observed for confirmation of histamine release. Passive cutaneous anaphylaxis (PCA) reactions were evaluated using an animal model and peiminine demonstrated inhibitory effects on IgE-dependent anaphylaxis. These results suggest that peiminine has regulatory potential for allergic inflammatory reactions mediated by HMC-1 cells.
[Mh] Termos MeSH primário: Antialérgicos/farmacologia
Cevanas/farmacologia
Liberação de Histamina/efeitos dos fármacos
Mastócitos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antialérgicos/administração & dosagem
Antialérgicos/química
Western Blotting
Técnicas de Cultura de Células
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Cevanas/administração & dosagem
Cevanas/química
Citocinas/imunologia
Ensaio de Imunoadsorção Enzimática
Liberação de Histamina/imunologia
Seres Humanos
Imunoglobulina E/imunologia
Mastócitos/imunologia
Anafilaxia Cutânea Passiva/imunologia
Ratos Sprague-Dawley
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Allergic Agents); 0 (Cevanes); 0 (Cytokines); 37341-29-0 (Immunoglobulin E); JKN43410XZ (peiminine)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150701
[St] Status:MEDLINE
[do] DOI:10.3109/08923973.2015.1059441



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