Base de dados : MEDLINE
Pesquisa : D03.132.960 [Categoria DeCS]
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[PMID]:29183753
[Au] Autor:Lee CH; Chu CS; Tsai HJ; Ke LY; Lee HC; Yeh JL; Chen CH; Wu BN
[Ad] Endereço:Department of Pharmacology, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
[Ti] Título:Xanthine-derived KMUP-1 reverses glucotoxicity-activated Kv channels through the cAMP/PKA signaling pathway in rat pancreatic ß cells.
[So] Source:Chem Biol Interact;279:171-176, 2018 Jan 05.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Hyperglycemia-associated glucotoxicity induces ß-cell dysfunction and a reduction in insulin secretion. Voltage-dependent K (Kv) channels in pancreatic ß-cells play a key role in glucose-dependent insulin secretion. KMUP-1, a xanthine derivative, has been demonstrated to modulate Kv channel activity in smooth muscles; however, the role of KMUP-1 in glucotoxicity-activated Kv channels in pancreatic ß-cells remains unclear. In this study we examined the mechanisms by which KMUP-1 could inhibit high glucose (25 mM) activated Kv currents (IKv) in pancreatic ß-cells. Pancreatic ß-cells were isolated from Wistar rats and IKv was monitored by perforated patch-clamp recording. The peak IKv in high glucose-treated ß-cells was ∼1.4-fold greater than for normal glucose (5.6 mM). KMUP-1 (1, 10, 30 µM) prevented high glucose-stimulated IKv in a concentration-dependent manner. Reduction of high glucose-activated IKv was also found for protein kinase A (PKA) activator 8-Br-cAMP (100 µM). Additionally, KMUP-1 (30 µM) current inhibition was reversed by the PKA inhibitor H-89 (1 µM). Otherwise, pretreatment with the PKC activator or inhibitor had no effect on IKv in high glucose exposure. In conclusion, glucotoxicity-diminished insulin secretion was due to IKv activation. KMUP-1 attenuated high glucose-stimulated IKv via the PKA but not the PKC signaling pathway. This finding provides evidence that KMUP-1 might be a promising agent for treating hyperglycemia-induced insulin resistance.
[Mh] Termos MeSH primário: Proteínas Quinases Dependentes de AMP Cíclico/metabolismo
AMP Cíclico/metabolismo
Glucose/toxicidade
Células Secretoras de Insulina/efeitos dos fármacos
Piperidinas/farmacologia
Canais de Potássio/metabolismo
Xantinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Insulina/secreção
Células Secretoras de Insulina/metabolismo
Ratos
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insulin); 0 (Piperidines); 0 (Potassium Channels); 0 (Xanthines); 1X0H7WEC5D (KMUP 1); E0399OZS9N (Cyclic AMP); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE


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[PMID]:28873557
[Au] Autor:Machado KN; Freitas AA; Cunha LH; Faraco AAG; Pádua RM; Braga FC; Vianna-Soares CD; Castilho RO
[Ad] Endereço:Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais (UFMG), Av. Pres. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil. Electronic address: kamilla.ufop@gmail.com.
[Ti] Título:A rapid simultaneous determination of methylxanthines and proanthocyanidins in Brazilian guaraná (Paullinia cupana Kunth.).
[So] Source:Food Chem;239:180-188, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Paullinia cupana is a plant native to Brazil that is widely used in traditional medicine as a physical and mental stimulant. It is also used worldwide to produce soft drinks. A method for the simultaneous quantitation of seven markers in guaraná by HPLC-PDA was developed, and extraction methods for the determination of methylxanthines and tannins were investigated. Quantified substances were theobromine, theophylline, caffeine, catechin, epicatechin, procyanidins A2 and B2. Results confirmed the satisfactory selectivity and linearity (r ≥0.99) within the mass ranges. Repeatability (RSD≤2.80%), intermediate precision (RSD≤4.47%), accuracy (recoveries from 90.59%-104.67%), and robustness were demonstrated. Extract 1 presented the contents: 0.0177% (±1.02%) for theobromine, 0.0131% (±1.14%) for theophylline, 2.9429% (±1.27%) for caffeine, 0.4563% (±1.02%) for catechin, 0.5515% (±1.05%) for epicatechin, 0.0607% (±2.80%) for A2 and 0.1035% (±1.39%) for B2. The method for simultaneous quantitation of seven chemical markers in guaraná proved to be reliable using a simple and convenient HPLC setup.
[Mh] Termos MeSH primário: Paullinia
[Mh] Termos MeSH secundário: Brasil
Cafeína
Extratos Vegetais
Proantocianidinas
Xantinas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Proanthocyanidins); 0 (Xanthines); 28109-92-4 (methylxanthine); 3G6A5W338E (Caffeine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


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[PMID]:28992566
[Au] Autor:Rodriguez A; Gomila RM; Martorell G; Costa-Bauza A; Grases F
[Ad] Endereço:Laboratory of Renal Lithiasis Research, University Institute of Health Sciences Research (IUNICS-IdISBa), University of Balearic Islands, Ctra Valldemossa, km 7.5. 07122 Palma de Mallorca, Spain. Electronic address: adrian.rodriguez@uib.es.
[Ti] Título:Quantification of xanthine- and uric acid-related compounds in urine using a "dilute-and-shoot" technique coupling ultra-high-performance liquid chromatography and high-resolution Orbitrap mass spectrometry.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1067:53-60, 2017 Nov 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Quantitative analysis of relevant metabolites in biofluids such as urine is often a tedious procedure, since it usually requires extraction, purification or preconcentration. For instance, in the analysis of methylxanthines in urine, a solid-phase extraction is often required. In the current work, a rapid and highly sensitive "dilute-and-shoot" method combining ultra-high-performance liquid chromatography and high-resolution mass spectrometry (UHPLC/HRMS) was validated for urinary determination of twelve analytes: uric acid, hypoxanthine, xanthine, 1-methyluric acid, 1,3-dimethyluric acid, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, theophylline, theobromine, paraxanthine and caffeine. These analytes are the major physiological metabolites of caffeine, theobromine or theophylline, or final products of purine catabolism. The separation was carried out on a core-shell Kinetek EVO C column coupled to a Q Exactive Orbitrap high-resolution mass spectrometer equipped with a heated electrospray ionization (HESI) probe, that operated both in positive and negative ionization modes. The twelve analytes eluted from between 1.5 and 10.5min. The lower limit of quantification (LLOQ) values ranged from 0.25 to 2.5ng/mL, and the calibration curves were linear from the LLOQ to 100ng/mL. The only pretreatment needed was to dilute each urine sample (typically to 1/500) with 0.1% formic acid solution, and then filter the diluted sample before injecting it into the UHPLC system. With this high dilution, there were no significant matrix effects, and the intra- and inter-day precision and accuracy values were acceptable (coefficients of variance and relative errors below 15%, except for the LLOQ, for which they were below 20%). Furthermore, the analysis of spiked urine samples with 25ng/mL of the target analytes showed excellent recoveries and precision levels for the twelve analytes. To our knowledge, there is no other published method that allows for the simultaneous determination of the concentrations of these twelve compounds, nor has a previously reported method been indicated to show such low LLOQ values as we have for the majority of the analytes. We expect our protocol to be useful for nutritional assessments, interventional studies, kidney stone research, and purine metabolism studies.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Espectrometria de Massas/métodos
Ácido Úrico/urina
Xantinas/urina
[Mh] Termos MeSH secundário: Seres Humanos
Limite de Detecção
Modelos Lineares
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Xanthines); 268B43MJ25 (Uric Acid)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171010
[St] Status:MEDLINE


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[PMID]:28950257
[Au] Autor:Zhou Y; Tong L; Chu X; Deng F; Tang J; Tang Y; Dai Y
[Ti] Título:The Adenosine A1 Receptor Antagonist DPCPX Inhibits Tumor Progression via the ERK/JNK Pathway in Renal Cell Carcinoma.
[So] Source:Cell Physiol Biochem;43(2):733-742, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: The adenosine A1 receptor (A1R) has been reported to be involved in the pathogenesis of various cancers, and the effects of A1R on different cancers are pleiotropic. However, the role of A1R in renal cell carcinoma (RCC) remains not well-known. METHODS: The expression of A1R in RCC cells was detected by quantitative real-time PCR and Western blotting analysis. Cell proliferation was detected using an MTT assay and a colony formation assay. Tumor growth was also evaluated in nude mice. Cell invasion and migration were evaluated using a wound healing assay and a transwell assay. Cell cycle distribution and apoptosis rates were analyzed by flow cytometry. RESULTS: A1R was the main subtype of ARs and was up-regulated in 786-O and ACHN cells. Functionally, 1,3-dipropyl-8-cyclopentylxanthine (DPCPX), an A1R antagonist, inhibited RCC cell proliferation in vitro and tumor growth in vivo. Furthermore, DPCPX inhibited RCC cell migration, while N6-Cyclopentyladenosine (CPA), a selective A1 agonist, was able to rescue RCC cell migration. In addition, DPCPX promoted 786-O and ACHN cell apoptosis and induced an S phase cell cycle arrest. Finally, we demonstrated that DPCPX inhibited tumor progression in part via the ERK/JNK pathway. CONCLUSION: These findings suggest the potentially important role of DPCPX in the control of RCC cell proliferation and migration by regulating the ERK/JNK signaling pathway.
[Mh] Termos MeSH primário: Antagonistas do Receptor A1 de Adenosina/uso terapêutico
Carcinoma de Células Renais/tratamento farmacológico
Neoplasias Renais/tratamento farmacológico
Rim/efeitos dos fármacos
MAP Quinase Quinase 4/metabolismo
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Xantinas/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Carcinoma de Células Renais/metabolismo
Carcinoma de Células Renais/patologia
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Seres Humanos
Rim/metabolismo
Rim/patologia
Neoplasias Renais/metabolismo
Neoplasias Renais/patologia
Camundongos Endogâmicos BALB C
Camundongos Nus
Receptor A1 de Adenosina/metabolismo
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adenosine A1 Receptor Antagonists); 0 (Receptor, Adenosine A1); 0 (Xanthines); 9PTP4FOI9E (1,3-dipropyl-8-cyclopentylxanthine); EC 2.7.12.2 (MAP Kinase Kinase 4)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170927
[St] Status:MEDLINE
[do] DOI:10.1159/000481557


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[PMID]:28945220
[Au] Autor:Schwarz Y; Zhao N; Kirchhoff F; Bruns D
[Ad] Endereço:Molecular Neurophysiology, Center for Integrative Physiology and Molecular Medicine, Saarland University, Homburg, Germany.
[Ti] Título:Astrocytes control synaptic strength by two distinct v-SNARE-dependent release pathways.
[So] Source:Nat Neurosci;20(11):1529-1539, 2017 Nov.
[Is] ISSN:1546-1726
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Communication between glia cells and neurons is crucial for brain functions, but the molecular mechanisms and functional consequences of gliotransmission remain enigmatic. Here we report that astrocytes express synaptobrevin II and cellubrevin as functionally non-overlapping vesicular SNARE proteins on glutamatergic vesicles and neuropeptide Y-containing large dense-core vesicles, respectively. Using individual null-mutants for Vamp2 (synaptobrevin II) and Vamp3 (cellubrevin), as well as the corresponding compound null-mutant for genes encoding both v-SNARE proteins, we delineate previously unrecognized individual v-SNARE dependencies of astrocytic release processes and their functional impact on neuronal signaling. Specifically, we show that astroglial cellubrevin-dependent neuropeptide Y secretion diminishes synaptic signaling, while synaptobrevin II-dependent glutamate release from astrocytes enhances synaptic signaling. Our experiments thereby uncover the molecular mechanisms of two distinct v-SNARE-dependent astrocytic release pathways that oppositely control synaptic strength at presynaptic sites, elucidating new avenues of communication between astrocytes and neurons.
[Mh] Termos MeSH primário: Astrócitos/secreção
Proteínas SNARE/secreção
Transdução de Sinais/fisiologia
Sinapses/secreção
[Mh] Termos MeSH secundário: Difosfato de Adenosina/farmacologia
Animais
Astrócitos/efeitos dos fármacos
Astrócitos/fisiologia
Células Cultivadas
Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos
Potenciais Pós-Sinápticos Excitadores/fisiologia
Feminino
Masculino
Camundongos
Camundongos Knockout
Camundongos Transgênicos
Técnicas de Cultura de Órgãos
Transdução de Sinais/efeitos dos fármacos
Sinapses/efeitos dos fármacos
Sinapses/fisiologia
Xantinas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (SNARE Proteins); 0 (Xanthines); 61D2G4IYVH (Adenosine Diphosphate); 9PTP4FOI9E (1,3-dipropyl-8-cyclopentylxanthine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.1038/nn.4647


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[PMID]:28895389
[Au] Autor:Czernicka M; Zagula G; Bajcar M; Saletnik B; Puchalski C
[Ad] Endereço:University of Rzeszow, Faculty of Biology and Agriculture, Department of Bioenergetics and Food Analysis, Rzeszow, Poland
[Ti] Título:Study of nutritional value of dried tea leaves and infusions of black, green and white teas from Chinese plantations
[So] Source:Rocz Panstw Zakl Hig;68(3):237-245, 2017.
[Is] ISSN:0035-7715
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Background: The processing of tea leaves determines the contents of bioactive ingredients, hence it should be expected that each variety of tea, black, red or green, will represent a different package of compounds of physiological importance. Taste and aroma, as well as price and brand are the main factors impacting consumers' preferences with regard to tea of their choice; on the other hand consumers less frequently pay attention to the chemical composition and nutritional value of tea. Objective: The purpose of the study was assessment of the nutritional value of black, green and white high-quality tea leaf from Chinese plantations based on the chemical composition of the dried leaves as well as minerals and caffeine content in tea infusions. Material and methods: The research material included 18 high-quality loose-leaf teas produced at Chinese plantations, imported to Poland, and purchased in an online store. The analyses included examination of the dried tea leaves for their chemical composition (contents of water, protein, volatile substances and ash) and assessment of selected minerals and caffeine contents in the tea infusions. Results: High-quality Chinese green teas were found with the most valuable composition of minerals, i.e. the highest contents of Zn, Mn, Mg, K, Ca and Al and the highest contents of protein in comparison to the other products. Chinese black teas had the highest contents of total ash and caffeine and white teas were characterized with high content of volatile substances, similar to the black teas, and the highest content of water and the lowest content of total ash. Conclusions: The three types of tea brews examined in the present study, in particular green tea beverages, significantly enhance the organism's mineral balance by providing valuable elements
[Mh] Termos MeSH primário: Antioxidantes/análise
Camellia sinensis/química
Flavonoides/análise
Minerais/análise
Fenóis/análise
Chá/química
[Mh] Termos MeSH secundário: Fibras na Dieta/análise
Seres Humanos
Valor Nutritivo
Extratos Vegetais/química
Folhas de Planta/química
Xantinas/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Dietary Fiber); 0 (Flavonoids); 0 (Minerals); 0 (Phenols); 0 (Plant Extracts); 0 (Tea); 0 (Xanthines)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE


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[PMID]:28871831
[Au] Autor:Mohamed AR; Georgey HH; George RF; El-Eraky WI; Saleh DO; Abdel Gawad NM
[Ad] Endereço:Pharmaceutical Chemistry Department, Faculty of Pharmacy, Egyptian Russian University, Badr City, Cairo 11829, Egypt.
[Ti] Título:Identification of some novel xanthine-based derivatives with bronchodilator activity.
[So] Source:Future Med Chem;9(15):1731-1747, 2017 Oct.
[Is] ISSN:1756-8927
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIM: The discovery of new bronchodilators with higher efficacy than theophylline is an important issue for asthmatic patients. MATERIALS & METHODS: Theophylline 2, 8-bromotheophylline 4 and theobromine 6 were reacted with different 2/3-chloro-N-phenylacetamides 1a-d or their propanamide analogs 1e-g to obtain 3a-g, 5a-g and 7a-g, respectively. The target compounds were screened for their in vitro bronchodilator activity using isolated guinea pig tracheal rings precontracted with histamine and compared with their precursors. RESULTS: Many compounds exhibited promising activity especially 3d, 3f, 5d, 7d and 7e. 2D-QSAR study resulted in a significant model (N = 24, n = 5, R  = 0.848, R cvOO = 0.748, R cvMO = 0.745, F = 21.215, s  = 0.0002) using CODESSA-Pro software. CONCLUSION:  These compounds can be considered as promising hits for potent bronchodilators that may be useful for further investigations. [Formula: see text].
[Mh] Termos MeSH primário: Broncodilatadores/química
Broncodilatadores/farmacologia
Xantinas/química
[Mh] Termos MeSH secundário: Animais
Broncoconstrição/efeitos dos fármacos
Broncodilatadores/síntese química
Cobaias
Histamina/toxicidade
Concentração Inibidora 50
Relação Quantitativa Estrutura-Atividade
Traqueia/efeitos dos fármacos
Traqueia/fisiologia
Xantinas/síntese química
Xantinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bronchodilator Agents); 0 (Xanthines); 820484N8I3 (Histamine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.4155/fmc-2017-0092


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[PMID]:28689812
[Au] Autor:Hasan AU; Kittikulsuth W; Yamaguchi F; Musarrat Ansary T; Rahman A; Shibayama Y; Nakano D; Hitomi H; Tokuda M; Nishiyama A
[Ad] Endereço:Department of Pharmacology, Faculty of Medicine, Kagawa University, 1750-1, Ikenobe, Kita-gun, Miki-cho, Kagawa 761-0793, Japan; Department of Pharmacology, Faculty of Medicine, International University of Health and Welfare, 4-2, Kojunomori, Narita-shi, Chiba 286-8686, Japan. Electronic address: ha
[Ti] Título:IBMX protects human proximal tubular epithelial cells from hypoxic stress through suppressing hypoxia-inducible factor-1α expression.
[So] Source:Exp Cell Res;358(2):343-351, 2017 Sep 15.
[Is] ISSN:1090-2422
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hypoxia predisposes renal fibrosis. This study was conducted to identify novel approaches to ameliorate the pathogenic effect of hypoxia. Using human proximal tubular epithelial cells we showed that a pan-phosphodiesterase (PDE) inhibitor, 3-isobutyl-1-methylxanthine (IBMX) dose and time dependently downregulated hypoxia-inducible factor 1α (HIF-1α) mRNA expression, which was further augmented by addition of a transcriptional inhibitor, actinomycin D. IBMX also increased the cellular cyclic adenosine monophosphate (cAMP) level. Luciferase assay showed that blocking of protein kinase A (PKA) using H89 reduced, while 8-Br-cAMP agonized the repression of HIF-1α promoter activity in hypoxic condition. Deletion of cAMP response element binding sites from the HIF-1α promoter abrogated the effect of IBMX. Western blot and immunofluorescent study confirmed that the CoCl induced increased HIF-1α protein in whole cell lysate and in nucleus was reduced by the IBMX. Through this process, IBMX attenuated both CoCl and hypoxia induced mRNA expressions of two pro-fibrogenic factors, platelet-derived growth factor B and lysyl oxidase. Moreover, IBMX reduced production of a mesenchymal transformation factor, ß-catenin; as well as protected against hypoxia induced cell-death. Taken together, our study showed novel evidence that the PDE inhibitor IBMX can downregulate the transcription of HIF-1α, and thus may attenuate hypoxia induced renal fibrosis.
[Mh] Termos MeSH primário: 1-Metil-3-Isobutilxantina/farmacologia
Células Epiteliais/efeitos dos fármacos
Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo
[Mh] Termos MeSH secundário: Hipóxia Celular/efeitos dos fármacos
Células Cultivadas
Proteínas Quinases Dependentes de AMP Cíclico/metabolismo
Células Epiteliais/metabolismo
Seres Humanos
Proteínas Proto-Oncogênicas c-sis/metabolismo
Transdução de Sinais/efeitos dos fármacos
Xantinas/farmacologia
beta Catenina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (CTNNB1 protein, human); 0 (Hypoxia-Inducible Factor 1, alpha Subunit); 0 (Proto-Oncogene Proteins c-sis); 0 (Xanthines); 0 (beta Catenin); 28109-92-4 (methylxanthine); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases); TBT296U68M (1-Methyl-3-isobutylxanthine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE


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[PMID]:28412808
[Au] Autor:Lin X; Gao X; Chen Z; Zhang Y; Luo W; Li X; Li B
[Ad] Endereço:College of Food Science, South China Agricultural University , 483 Wushan Street, Tianhe District, Guangzhou 510642, People's Republic of China.
[Ti] Título:Spontaneously Assembled Nano-aggregates in Clear Green Tea Infusions from Camellia ptilophylla and Camellia sinensis.
[So] Source:J Agric Food Chem;65(18):3757-3766, 2017 May 10.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tea nano-aggregates spontaneously assembled in clear tea infusions are considered as the precursors of tea cream, although their molecular basis remains obscure. Here, we characterized nano-aggregates in green tea infusions from Camellia ptilophylla, a peculiar tea variety with 6.0% of theobromine, and Camellia sinensis as the control for comparative purpose. Numerous negatively charged spherical colloidal particles of 50-100 nm in diameter were primarily found in both green tea infusions. Catechins, proteins, and carbohydrates were confirmed as the dominant components in green tea nano-aggregates. In addition, iron, copper, nickel, proteins, and gallated catechins exhibited higher aggregating affinity than other components, whereas methylxanthines and calcium contributed to the transformation of nano-aggregates into tea cream. Green tea nano-aggregates were partly destroyed by simulated gastrointestinal digestion, and removing theses peculiar particles dramatically attenuated the bioaccessibility of methylxanthines, theanine, and some catechin monomers in green tea infusions. This study enhanced our knowledge of molecular interactions in the formation of green tea cream and provided insight into physicochemical profiles, phytochemical nature, and functional effects of green tea nano-aggregates.
[Mh] Termos MeSH primário: Camellia sinensis/química
Camellia/química
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Catequina/química
Nanopartículas/química
Folhas de Planta/química
Xantinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Xanthines); 28109-92-4 (methylxanthine); 8R1V1STN48 (Catechin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b00068


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[PMID]:28282918
[Au] Autor:Basnet RM; Guarienti M; Memo M
[Ad] Endereço:Department of Molecular and Translational Medicine, University of Brescia, Viale Europa, 11, 25123 Brescia, Italy. r.basnet@unibs.it.
[Ti] Título:Zebrafish Embryo as an In Vivo Model for Behavioral and Pharmacological Characterization of Methylxanthine Drugs.
[So] Source:Int J Mol Sci;18(3), 2017 Mar 09.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Zebrafish embryo is emerging as an important tool for behavior analysis as well as toxicity testing. In this study, we compared the effect of nine different methylxanthine drugs using zebrafish embryo as a model. We performed behavioral analysis, biochemical assay and Fish Embryo Toxicity (FET) test in zebrafish embryos after treatment with methylxanthines. Each drug appeared to behave in different ways and showed a distinct pattern of results. Embryos treated with seven out of nine methylxanthines exhibited epileptic-like pattern of movements, the severity of which varied with drugs and doses used. Cyclic AMP measurement showed that, despite of a significant increase in cAMP with some compounds, it was unrelated to the observed movement behavior changes. FET test showed a different pattern of toxicity with different methylxanthines. Each drug could be distinguished from the other based on its effect on mortality, morphological defects and teratogenic effects. In addition, there was a strong positive correlation between the toxic doses (TC ) calculated in zebrafish embryos and lethal doses (LD ) in rodents obtained from TOXNET database. Taken together, all these findings elucidate the potentiality of zebrafish embryos as an in vivo model for behavioral and toxicity testing of methylxanthines and other related compounds.
[Mh] Termos MeSH primário: Embrião não Mamífero/efeitos dos fármacos
Teratogênios/toxicidade
Testes de Toxicidade/métodos
Xantinas/toxicidade
[Mh] Termos MeSH secundário: Animais
AMP Cíclico/metabolismo
Locomoção
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Teratogens); 0 (Xanthines); 28109-92-4 (methylxanthine); E0399OZS9N (Cyclic AMP)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170420
[Lr] Data última revisão:
170420
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170312
[St] Status:MEDLINE



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